Cell culture bag assembly suitable for automated medium exchange and transportation

Abstract

A cell culture bag assembly suitable for automatic medium replacement and transportation includes: a frame comprising a base, a plurality of columns extending upward from the base, and a frame connected to the upper end of the columns; the frame defines an accommodating space; an inclined plate is provided in the accommodating space, the inclined plate having a high end portion at a relatively high position and a low end portion at a relatively low position; a first tube-holding groove is recessed at one of the columns adjacent to the low end portion; a cell bag is disposed in the accommodating space, the upper end of the cell bag is suspended from the upper frame, and the lower end of the cell bag forms an inclined bottom surface that adheres to the inclined plate; a suction tube is connected to the cell bag near the inclined bottom surface and engages with the first tube-holding groove, so that the cultured cells in the bag can concentrate towards the lower position after standing, which is beneficial for rapid extraction during cell harvesting.

Claims

1. A cell culture bag assembly suitable for automated medium change and transport, comprising: A frame includes a base, a plurality of columns extending upward from the base, and an upper frame connected to the upper end of the columns. The base, the columns, and the upper frame together define an accommodating space. The base is provided with an inclined plate within the accommodating space, and the inclined plate has a high end portion at a relatively high position and a low end portion at a relatively low position. A first tube-holding groove is recessed at one of the columns near the low end portion. A cell bag is disposed within the accommodating space. The upper end of the cell bag is suspended from the upper frame, and the lower end of the cell bag forms an inclined bottom surface. The inclined bottom surface is stretched and placed on the inclined plate by the suspension. A liquid extraction tube is connected to the cell bag at the lowest point near the inclined bottom surface, and the liquid extraction tube is engaged with the first tube-holding groove and fixed to the frame.

2. The cell culture bag assembly for automatic fluid exchange and transport as described in claim 1, wherein the upper frame is provided with a plurality of hooks and the upper end of the cell bag is provided with a plurality of straps, the straps being hooked onto the hooks respectively, so that the cell bag body, except for the inclined bottom surface being placed on the inclined plate, does not directly contact the base, the column and the upper frame.

3. The cell culture bag assembly for automatic fluid exchange and transport as described in claim 1, wherein the column is recessed with a second tube-holding groove between the first tube-holding groove and the upper frame, and a detection tube is connected to the cell bag above the position of the extraction tube, and the detection tube is engaged with the second tube-holding groove.

4. The cell culture bag assembly for automatic fluid exchange and transport as described in claim 3, wherein a slot is recessed on the inner edge of the upper frame and a cover plate is attached to the slot so that the cover plate covers the upper part of the accommodating space.

5. The cell culture bag assembly for automatic fluid exchange and transport as described in claim 4, wherein the cover plate is recessed with a third tube clamping groove, a feed tube is connected to the upper end of the cell bag, one end of the feed tube is clamped in the third tube clamping groove, and the other end extends into the cell bag and is adjacent to the sloping bottom surface.

6. The cell culture bag assembly for automated fluid exchange and transport as described in claim 5, wherein the aspiration tube, the detection tube, and the feed tube are each connected to a Luer taper.

7. The cell culture bag assembly for automatic fluid exchange and transport as described in claim 5, wherein the cover plate is recessed with a fourth tube clamping groove, the upper end of the cell bag is connected to a venting tube, one end of the venting tube is clamped in the fourth tube clamping groove, and the outer end of the venting tube is fitted with a cap having a waterproof and breathable membrane.

8. The cell culture bag assembly for automatic fluid exchange and transport as described in claim 1, wherein the lower end of the inclined plate is pivotally connected to the base, the column forms a stop for the upper end of the inclined plate to abut and be positioned, and the inclined plate can be pushed upward by an external force and swing around the lower end as a pivot point to push against the inclined bottom surface placed on the inclined plate, thereby reducing the amount of residual cells and improving the stability of fluid extraction.

9. The cell culture bag assembly for automated medium change and transport as described in claim 1, wherein the frame is placed on a magnetic levitation trolley, the magnetic levitation trolley is equipped with a plurality of magnetically conductive blocks, and the magnetic levitation trolley is suspended above at least one stator platform, the stator platform is equipped with a plurality of electromagnetic coils, the electromagnetic coils generating a controllable magnetic field to drive the magnetic levitation trolley to perform at least six degrees of freedom of motion, so that the magnetic levitation trolley carries the frame and the cell bag in a non-contact support state relative to the stator platform for transport, shaking and medium change positioning.

10. The cell culture bag assembly for automated fluid exchange and transport as described in claim 9, wherein two clamping portions are symmetrically protruding on opposite sides of the frame, the two clamping portions being used by at least one robotic arm to clamp and pick up the frame to perform transfer or positioning operations.

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