Collagen enzymatic hydrolysis system

Abstract

A collagen enzymatic hydrolysis system includes: a gelling device configured to gelatinize collagen material to obtain crude collagen solution; a separation device connected to the gelling device configured to separate the crude collagen solution to remove macromolecular proteins and impurities, thereby obtaining a refined collagen solution; an enzymatic hydrolysis device connected to the separation device configured to mix the refined collagen solution with a protease solution for enzymatic hydrolysis to obtain a polypeptide product; a purification device connected to the enzymatic hydrolysis device configured to purify the polypeptide product, thereby obtaining a purified polypeptide product; and a drying device connected to the purification device configured to dry the purified polypeptide product, thereby obtaining a dried purified polypeptide product.

Description

Collagen enzymatic hydrolysis system Technical Field

[0001] This work relates to an enzymatic hydrolysis system, and more particularly to a collagen hydrolysis system. Prior Technology

[0002] Deer antler glue, rich in collagen and functional peptides, has wide applications in the pharmaceutical, health product, and cosmetic fields. However, current technologies still suffer from insufficient purification specificity in the preparation and application of peptides.

[0003] Therefore, there is a need for an extraction system for deer antler glue that can extract high-efficiency peptide products with high specificity. Summary of the Invention

[0004] Therefore, the purpose of this invention is to provide a collagenase hydrolysis system that can prepare peptide products with antioxidant effects and high stability.

[0005] This invention provides a collagen enzymatic hydrolysis system to address the problems of prior art. The system comprises: a gelation device configured to gelatinize collagen material to obtain crude collagen solution; a separation device connected to the gelation device configured to separate the crude collagen solution, removing large protein molecules and impurities to obtain a refined collagen solution; an enzymatic hydrolysis device connected to the separation device configured to mix the refined collagen solution with a protease solution for enzymatic hydrolysis to obtain a polypeptide product; a purification device connected to the enzymatic hydrolysis device configured to purify the polypeptide product to obtain a purified polypeptide product; and a drying device connected to the purification device configured to dry the purified polypeptide product to obtain a dried purified polypeptide product.

[0006] In one embodiment of this invention, a collagen enzymatic hydrolysis system is provided, wherein the separation device includes a centrifugation mechanism connected to the gelling device and configured to perform centrifugation processing.

[0007] In one embodiment of this invention, a collagen enzymatic hydrolysis system is provided, wherein the separation device includes a salting-out mechanism connected to the centrifugation mechanism and configured to perform salting-out treatment.

[0008] In one embodiment of this invention, a collagen enzymatic hydrolysis system is provided, wherein the purification device includes an ultrafiltration mechanism connected to the enzymatic hydrolysis device. The ultrafiltration mechanism includes a first filter membrane with a pore size range of 0.01 to 0.05 micrometers and a second filter membrane with a molecular weight cutoff range of 0.5 to 2 kDa, and is configured to perform ultrafiltration treatment.

[0009] In one embodiment of this invention, a collagen enzymatic hydrolysis system is provided, wherein the purification device includes a chromatography mechanism connected to the ultrafiltration mechanism and configured to perform chromatography processing.

[0010] In one embodiment of this invention, a collagen enzymatic hydrolysis system is provided, which further includes a pulverizing device connected to the gelling device, configured to pulverize the collagen material before feeding it into the gelling device.

[0011] In one embodiment of this invention, a collagenase hydrolysis system is provided, wherein the protease solution contains modified pepsin and trypsin, wherein the modified pepsin has D212 and E240 sites replaced with T212 and Q240, respectively.

[0012] The collagen enzymatic hydrolysis system developed in this invention enables the preparation of peptide products with antioxidant effects and high stability. Simple Explanation of the Diagram

[0013] Figure 1 is a schematic diagram showing a collagenase hydrolysis system according to a first embodiment of the present invention. Figure 2 is a schematic diagram showing a collagenase hydrolysis system according to another embodiment of the present invention. Figure 3 is a bar graph showing the free radical scavenging results of the products obtained from the collagenase hydrolysis system according to the first embodiment of the present invention. Figure 4 is a line graph showing the stability test results of the product obtained by the collagen hydrolysis system according to the first embodiment of the present invention. Implementation

[0014] The following description, based on Figures 1 to 4, illustrates an embodiment of the invention. This description is not intended to limit the implementation of the invention, but rather to provide one possible embodiment.

[0015] As shown in Figure 1, a collagen enzymatic hydrolysis system 100 according to an embodiment of the present invention includes: a gelling device 1, configured to gelleave collagen material to obtain crude collagen liquid; a separation device 2, connected to the gelling device 1, configured to separate the crude collagen liquid to remove macromolecular proteins and impurities, thereby obtaining a refined collagen solution; an enzymatic hydrolysis device 3, connected to the separation device 2, configured to mix the refined collagen solution with a protease solution for enzymatic hydrolysis to obtain a polypeptide product; a purification device 4, connected to the enzymatic hydrolysis device 3, configured to purify the polypeptide product, thereby obtaining a purified polypeptide product; and a drying device 5, connected to the purification device 4, configured to dry the purified polypeptide product, thereby obtaining a dried purified polypeptide product.

[0016] As shown in Figure 1, a collagen hydrolysis system 100 according to an embodiment of the present invention includes a separation device 2 comprising a centrifugation mechanism 21 connected to the gelling device 1 and configured to perform centrifugation processing.

[0017] As shown in Figure 1, a collagen hydrolysis system 100 according to an embodiment of the present invention includes a separation device 2 comprising a salting-out mechanism 22 connected to the centrifugation mechanism 21 and configured to perform salting-out treatment.

[0018] As shown in Figure 1, a collagen enzymatic hydrolysis system 100 according to an embodiment of the present invention includes a purification device 4 comprising an ultrafiltration mechanism 41 connected to the enzymatic hydrolysis device 3. The ultrafiltration mechanism 41 comprises a first filter membrane with a pore size range of 0.01 to 0.05 micrometers and a second filter membrane with a molecular weight cutoff range of 0.5 to 2 kDa, and is configured to perform ultrafiltration treatment.

[0019] As shown in Figure 1, a collagen enzymatic hydrolysis system 100 according to an embodiment of the present invention includes a purification device 4 comprising a chromatography mechanism 42 connected to an ultrafiltration mechanism 41 and configured to perform chromatography processing.

[0020] As shown in Figure 2, the collagen hydrolysis system 101 according to another embodiment of the present invention further includes a pulverizing device 6 connected to the gelling device 1, which is configured to pulverize the collagen material and feed it into the gelling device 1.

[0021] According to an embodiment of the present invention, a collagenase hydrolysis system 100 is provided, wherein the protease solution contains modified pepsin and trypsin, wherein the modified pepsin is wherein the D212 and E240 sites are replaced with T212 and Q240, respectively.

[0022] According to one embodiment of the present invention, a collagen enzymatic hydrolysis system 100 is provided to perform a 1,1-diphenyl-2-trinitrophenylhydrazine radical (2,2-Diphenyl-1-picrylhydrazy, DPPH) scavenging experiment on the produced polypeptide product. In this experiment, deer antler is used as the collagen material, and deer antler glue extracted by conventional technology but not enzymatically hydrolyzed is used as the control group. The experimental results are shown in Figure 3.

[0023] The results in Figure 3 show that, according to one embodiment of the present invention, the half-maximal inhibitory concentration (IC50) of the polypeptide product produced by the collagen enzymatic hydrolysis system 100 is less than 0.5 mg / mL, which is significantly better than the unhydrolyzed deer antler glue control group, indicating that it has excellent free radical scavenging ability.

[0024] According to one embodiment of the present invention, a collagen enzymatic hydrolysis system 100 is provided. The durability of the produced polypeptide products is tested, and the degradation results at room temperature are analyzed. The results are shown in Figure 4.

[0025] The results in Figure 4 show that, according to one embodiment of the present invention, when the peptide product produced by the collagen hydrolysis system 100 is stored at room temperature for 24 months, the degradation level gradually increases from 0% to 3%, which is significantly lower than the acceptable degradation level standard shown by the red dashed line.

[0026] Although the above embodiments use deer antler as the source material for collagen, this invention is not limited to this, and other collagen materials can also be applied to the system of this invention.

[0027] The above description and explanation are merely illustrative of preferred embodiments of this invention. Those skilled in the art may make other modifications based on the following defined scope of the patent application and the above description, but such modifications shall still be within the spirit of this invention and the scope of its rights.

[0028] 100, 101: Collagen enzymatic hydrolysis system 1: Gelatinizing device 2: Separation device 21: Centrifuge mechanism 22: Salting-out institution 3: Enzymatic hydrolysis device 4: Purification device 41: Ultrafiltration Mechanism 42: Chromatography mechanism 5: Drying device 6: Crushing device

Claims

1. A collagen enzymatic hydrolysis system, comprising: a gelling device configured to gelleave collagen material to obtain crude collagen solution; a separation device connected to the gelling device configured to separate the crude collagen solution to remove macromolecular proteins and impurities, thereby obtaining a refined collagen solution; an enzymatic hydrolysis device connected to the separation device configured to mix the refined collagen solution with a protease solution for enzymatic hydrolysis to obtain a polypeptide product; a purification device connected to the enzymatic hydrolysis device configured to purify the polypeptide product, thereby obtaining a purified polypeptide product; and a drying device connected to the purification device configured to dry the purified polypeptide product, thereby obtaining a dried purified polypeptide product.

2. The collagen enzymatic hydrolysis system as claimed in claim 1, wherein the separation device includes a centrifugation mechanism connected to the gelling device and configured to perform centrifugation.

3. The collagenase hydrolysis system as claimed in claim 2, wherein the separation device includes a salting-out mechanism connected to the centrifugation mechanism and configured to perform salting-out treatment.

4. The collagen enzymatic hydrolysis system as claimed in claim 3, wherein the purification device includes an ultrafiltration mechanism connected to the enzymatic hydrolysis device, the ultrafiltration mechanism including a first filter membrane with a pore size range of 0.01 to 0.05 micrometers and a second filter membrane with a molecular weight cutoff range of 0.5 to 2 kDa, and configured to perform ultrafiltration treatment.

5. The collagenase hydrolysis system as claimed in claim 4, wherein the purification apparatus includes a chromatography unit connected to the ultrafiltration unit and configured to perform chromatography.

6. The collagen enzymatic hydrolysis system as claimed in claim 1, further comprising a pulverizing device connected to the gelling device, configured to pulverize the collagen material for feeding into the gelling device.

7. The collagenase hydrolysis system of claim 1, wherein the protease solution contains modified pepsin and trypsin, wherein the modified pepsin is wherein the D212 and E240 sites are replaced with T212 and Q240, respectively.

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