SYNTHESIS PROCESS OF A POLY(LACTIC-CO-GLYCOLIC ACID) (PLGA) BIODEGRADABLE NANOSYSTEM CARRYING A MIXTURE OF DIOSMIN AND HESPERIDIN, COATED WITH CHITOSAN FOR ORAL TREATMENT OF VARICOSE VEINS

Abstract

The invention relates to a process for synthesizing a biodegradable nanoparticle, specifically poly(lactic-co-glycolic acid) (PLGA) carrying a diosmin-hesperidin drug mixture, by solvent evaporation and coated with chitosan for the oral treatment of varicose veins. The synthesis process consists of the following steps: creating a dispersed phase consisting of 0.5-7.5 ml of DMSO solvent dissolving the diosmin-hesperidin drug system (in a 9:1 mass ratio), mixed with 5-10 ml of ethyl acetate solution containing 30-75 mg of PLGA; and a 20 ml continuous phase consisting of 0-0.15% (weight / volume) chitosan mixed with 0.25-2% (weight / weight) polyvinylic alcohol to form a clear and homogeneous mixture. Next, slowly add the dispersed phase to the continuous phase, stirring vigorously at 300-500 rpm for 3-5 minutes, then homogenize for 1-5 minutes, stirring overnight at room temperature; centrifuge at 10000-15000 rpm for 30-60 minutes, rinse 3-5 times with distilled water and cool to a dry state (24 to 48 hours) to recover the solid powder.