SIDE-FLOW NUCLEIC ACID EXTRACTION DEVICE AND ITS MANUFACTURING PROCESS

Abstract

The invention relates to a side-flow nucleic acid extraction device and a process for manufacturing such a device, applied to the rapid extraction of nucleic acids (DNA / RNA) by integrating functional regions on a nitrocellulose substrate, including a lysis region and a capture region using a glass fiber membrane modified by cold plasma technology. The device according to the invention allows for the sequential execution of steps from cell / virus disruption to genetic material recovery in a relatively short time, approximately 15 to 20 minutes. A key feature of the invention is its automatic operation thanks to capillary action, completely eliminating the need for centrifuges or complex peripheral equipment, resulting in the recovery of highly pure nucleic acids that meet the criteria for replacing existing commercial kits. The side-flow nucleic acid extraction device is suitable for applications in genetic analysis, in-situ diagnosis, and field pathogen surveillance at low cost and with high stability.

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