FORMULATION COMPRISING A PROTEIN WITH INSECTICIDAL AND / OR NEMATICIDAL ACTIVITY AND RELATED METHODS

Claims

A formulation comprising a protein with pesticidal activity, useful for a pesticide formulation, and a method for controlling pests comprising the use of said formulation are disclosed, wherein the pesticide protein is a Txp40 protein or a variant thereof.

1. A pesticide formulation comprising: i) a polypeptide comprising a sequence as indicated in SEQ ID No. 1 or a variant thereof having at least 90% identity with the sequence as indicated in SEQ ID No. 1, ii) a pH buffer selected from HEPES, MOPS, PIPES, Tricine, Bicine, TES, Tris-HCl and Phosphate buffer, wherein the buffer has a pH value between 7 and 9; iii) a chelating agent selected from citric acid, ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), hydroxyethylenediaminetetraacetic acid (HEEDTA), ethylenediamine-[o-hydroxyphenylacetic] acid (EDDHA), ethylene glycol-bis-(2-aminoethyl ether)-tetraacetic acid (EGTA), diethylenetriaminepentacetic acid (DTPA) and 1,2-diaminocyclohexanetetraacetic acid (DCTA), iv) an emulsifier or a non-ionic surfactant selected from polysorbate, octoxinol, glycerol, sorbitan esters, polyethylene glycol (PEG) or polyvinyl alcohol, or combinations thereof;(v) a protease inhibitor selected from phenylmethylsulfonyl fluoride (PMSF), 4-(2-aminoethyl)benzene sulfonyl fluoride hydrochloride (AEBSF), E-64 and Pepstatin; and (vi) a preservative with biocidal activity selected from sodium azide, benzoisothiazolinone (BIT) and bronopol.