POLYPEPTIDES THAT BIND TO MIRO1

Claims

This disclosure describes anti-Miro1 polypeptides or their binding fragments, and nucleic acids, compositions, methods, kits, and systems related to the polypeptides or the binding fragments. In one aspect, the anti-Miro1 polypeptides or their binding fragments can be used as capture and detection reagents in a Miro1 ELISA. The Miro1 ELISA, as described herein, is capable of selectively detecting the Miro1 protein over the Miro2 protein.

1. An anti-Miro1 polypeptide or its binding fragment comprising (i) a VH chain comprising three CDR regions; and (ii) a VL chain comprising three CDR regions, wherein: VH CDR #1 is: SYAVR (SEQ ID No. 21) or SNAIS (SEQ ID No. 41); VH CDR #2 is: IISDSGTTYYANWAIG (SEQ ID No. 22) or FIGYTGNTYYATWAKG (SEQ ID No. 42); VH CDR #3 is: YDDYGDSILHI (SEQ ID No. 23) or GSAWLDP (SEQ ID No. 43); VL CDR #1 is: QASQNIDSNLV (SEQ ID No. 24) or QSSQSVYTNTRLS (SEQ ID No. 44); VL CDR #2 is: AASNLAS (SEQ ID NO: 25) or KASTLAS (SEQ ID NO: 45); and VL CDR #3 is: QCTYYSSSYVEV (SEQ ID NO: 26) or LGVYSGNIAV (SEQ ID NO: 46).

14. An anti-Miro1 polypeptide or its binding fragment comprising (i) a VH chain comprising three CDR regions; and (ii) a VL chain comprising three CDR regions; which is at least 3x, at least 10x, at least 100x, or at least 1000x selective with respect to Miro2.

15. A composition comprising the anti-Miro1 polypeptide or its binding fragment, according to any of claims 1 to 14, and an acceptable excipient.

16. A nucleic acid comprising a nucleotide sequence encoding an anti-Miro1 polypeptide or its binding fragment according to any of claims 1 to 14.

17. A vector comprising the nucleic acid of claim 16.

18. A host cell engineered to express the nucleic acid according to claim 16.

19. A host cell transformed with the vector according to claim 17.