ANTIBODIES AGAINST CD25, ANTIBODY-DRUG CONJUGATES AND THEIR USES

Claims

Antigen-binding proteins, such as antibodies and antibody fragments, that bind to CD25. Also provided are nucleic acids encoding such antigen-binding proteins, and vectors and cells useful for preparing such antigen-binding proteins. Antibody-drug conjugates comprising such antigen-binding proteins are also provided. Antigen-binding proteins and antibody-drug conjugates are useful in a variety of approaches, including the treatment of CD25-expressing and CD25-nonexpressing tumors.

1. An antigen binding protein that binds CD25 characterized in that it comprises: (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1; (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 2; (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 21; (d) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 4; (e) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 5; and (f) a CDR-L3 comprising the amino acid sequence of SEQ ID NO:

6.

12. An antibody-drug conjugate comprising the antigen-binding protein according to claim 1, characterized in that the antigen-binding protein is conjugated to a cytotoxic or cytostatic agent.

21. An antibody-drug conjugate characterized in that it comprises: formula (1), wherein Ab is an antigen-binding protein that binds to CD25 and comprises: (a) a CDR-H1 comprising the amino acid sequence of SEQ ID No. 1; (b) a CDR-H2 comprising the amino acid sequence of SEQ ID No. 2; (c) a CDR-H3 comprising the amino acid sequence of SEQ ID No. 21; (d) a CDR-L1 comprising the amino acid sequence of SEQ ID No. 4; (e) a CDR-L2 comprising the amino acid sequence of SEQ ID No. 5; and (f) a CDR-L3 comprising the amino acid sequence of SEQ ID No. 6, and wherein p is from 1 to 8.

26. An isolated nucleic acid characterized in that it encodes the antigen-binding protein according to claim 1.

27. A vector characterized in that it comprises the nucleic acid according to claim 26.

28. A host cell characterized in that it comprises the vector according to claim 27.

29. A method for producing an antigen-binding protein that binds to CD25, characterized in that the method comprises: a) culturing the host cell according to claim 28 under conditions suitable for the expression of the polynucleotide encoding the antigen-binding protein; and b) isolating the antigen-binding protein.

30. A method for producing an antibody-drug conjugate characterized in that it comprises an antigen-binding protein that binds to CD25, wherein the method comprises: a) culturing the host cell according to any of claim 28 under conditions suitable for the expression of the polynucleotide encoding the antigen-binding protein; b) isolating the antigen-binding protein; and c) conjugating the antigen-binding protein with a cytotoxic or cytostatic agent, wherein each unit of the cytotoxic or cytostatic agent is conjugated by means of a ligand.