Verfahren zur kompensation der variation eines fluoreszenzfarbstoffs durch fluoreszenzmessung im freien zustand

Abstract

The present invention relates to a method of determining a compensation factor to compensate for variations of fluorescence radiant intensity of a reagent containing a fluorochrome, the compensation factor being intended to be applied during a bound state fluorescence radiant intensity measurement when a cell is labeled with the fluorochrome, the method comprising at least the steps of: - measuring a free state fluorescence radiant intensity emitted by the reagent containing the fluorochrome when not labeled to a cell prior to the bound state fluorescence radiant intensity measurement, - determining the compensation factor from the measured free state fluorescence radiant intensity.