Pharmaceutical compositions of WNT pathway inhibitors for wound treatment

Abstract

A pharmaceutical composition comprising a WNT inhibitor, a glycol, one or more solubilizers, and saline or water. The pharmaceutical composition optionally comprises a graphene oxide-hyaluronic acid conjugate, one or more phospholipids, one or more carriers, stabilizer, buffer, and / or preservative. The pharmaceutical composition is suitable for topical administration.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] [RESERVED] FIELD

[0002] One or more embodiments of the present disclosure described herein relates to pharmaceutical compositions and methods for treatment of disease using the same. BACKGROUND

[0003] All publications, patents and patent applications cited within this application are herein incorporated by reference in their entirety to the same extent as if the disclosure of each individual publication, patent application or patent was specifically and individually indicated to be incorporated by reference in its entirety.

[0004] The Wnt pathway has been shown to play a role in dermal fibrosis and scarring. The Wnt pathway is an evolutionary conserved pathway that regulates crucial aspects of cell fate determination, cell polarity, cell migration, neural patterning, and organogenesis during embryonic development. This pathway plays a role in ensuring proper tissue development in embryos and tissue maintenance in adults. Wnt signaling is involved at the beginning stages of skin development, where following gastrulation, embryonic cells of the ectoderm and the mesoderm differentiate to form the epidermis and dermis, respectively.

[0005] Although there are at least three distinct Wnt signaling pathways involved in the signal transduction process, the canonical (or P-catenin dependent) Wnt pathway is well understood. P-Catenin is an effector molecule resulting from the signaling of the canonical Wnt pathway, and its protein levels are regulated through a "destruction complex". In the absence of a Wnt signal, the transcriptional activator P-catenin is actively degraded in the cell by the actions of a protein complex, designated the "destruction complex". Within this complex, Axin-1 and -2 with adenomatous polypsis coli form a scaffold that facilitates P-catenin phosphorylation by casein-kinase 19a and glycogen synthase kinase 3p. Phosphorylated P-catenin is recognized and ubiquitinylated, resulting in its proteosomal degradation. Tankryase I and II (TK1 and TK2) are poly(ADP-ribose) polymerases (PARPs) that function to parsylate and destabilize Axin-1 and -2 proteins, thus destabilizing the P-catenin destruction complex. Once the destruction complex is destabilized, this allows P-catenin to be dephosphorylated, and subsequently stabilized and allowed to accumulate in the cytoplasm and enter the cell nucleus, where it interacts with members of the Tcf / Lef family. P-catenin converts the Tcf proteins into potent transcriptional activators by recruiting co-activator proteins, thus ensuring efficient activation of Wnt target genes. The Wnt pathway, once activated by the Wnt family of natural ligands, upregulates TNK1 and TNK2 to help destabilize the destruction complex. Studies have shown that TNK1 and TNK2 are regulators of canonical Wnt signaling.

[0006] XAV939 is a small molecule that selectively inhibits Wnt / p-catenin-mediated transcription through TK 1 and TK2 inhibition with an IC50 of 11 nM / 4nM in cell-free assays, regulates axin levels, and does not affect CRE, F-kB, or TGF-p. Recently, topical application of XAV939 in a mouse ear punch assay demonstrated that XAV939 significantly increased rate of wound closure with reduced fibrosis (scarring). However, XAV939 was dissolved in dimethyl sulfoxide (DMSO) and used as a “research tool” compound due to its low aqueous solubility (<1 pg / mL). The problem with this approach is that humans cannot tolerate the use of DMSO.

[0007] The prior art has shown the utility of a matrix component comprising graphene oxide (GO) and hyaluronic acid (HA) as being effective in both providing a supportive matrix for XAV939 and allowing the use of XAV939 as a therapeutic for wound healing in humans and animals, see for example US20210000959, where XAV939 in a GO-HA matrix provides substantial improvement to the quality of wound healing; specifically causing the tissues to limit scarring following a fibrotic wound healing pathway. Further, the prior art has reported the observation of increased cartilage regeneration and healing following acute injury, for example by way of a 2mm biopsy punch wound made in the center of the cartilaginous region of a C57B1 / CJ mouse, by way of administration of XAV939 dissolved in DMSO (Bastakoty, D. et al. 2015, 29(12): 4881-4892).

[0008] While the utility of XAV939 to improve the quality of regeneration of tissue following injury, including the regeneration of cartilage, and while reducing the healing by way of fibrotic mechanisms which results in scarring has been demonstrated, XAV939 has poor solubility. While XAV939 inhibits the Wnt pathway, there is a need for improved Wnt inhibitors with improved characteristics such as increased solubility in water, increased Wnt inhibition, increased bioavailability or modified biological stability once administered to a patient in need. BRIEF DESCRIPTION OF DRAWINGS

[0009] FIG. 1 is a bar graph showing results (scores) of a pharmaceutical composition according to one or more embodiments, Formulation 1, vs vehicle in re-epithelization, neovascularization, rete ridge, scabbing, and granulation (Biological Example 1 A).

[0010] FIG. 2 is a plot showing the average area size of the wounds of the vehicle group and the Formulation 1 group during the course of a 21-day treatment. The results indicate that topical administration of Formulation 1 increased the rate of healing of a diabetic rat impaired healing animal model compared to vehicle (Biological Example 1 A).

[0011] FIG. 3 shows the distance between opposing cartilage endpoints in mice from a 30-day treatment of 2 millimeter (mm) biopsy punch wounds with: dimethylsulfoxide (DMSO) control; saline and XAV939 in DMSO (XAV939); and a 15-day treatment with GO-HA, and a 30-day treatment of pharmaceutical composition according to one or more embodiments, Formulation 1 (Biological Example IB).

[0012] FIG. 4 shows the distance between opposing cartilage endpoints in mice as between 30-day treatment of 2 mm biopsy punch wounds with XAV939 in DMSO and 15-day treatment with Formulation 1 (Biological Example IB).

[0013] FIG. 5 shows the distance between opposing cartilage endpoints in mice from a 15-day treatment of 2 mm biopsy punch wounds with GO-HA and a 15-day treatment with Formulation 1 (Biological Example IB).

[0014] FIG. 6 shows the distance between opposing cartilage endpoints in rabbits from a 21-day treatment of 6 mm biopsy punch wounds with saline and a 21-day treatment with Formulation 2 (Biological Example 2).

[0015] FIG. 7 shows inhibition of the Wnt transcription signaling pathway activity by a Wnt inhibitor of Formula (I) (Compound 1 in DMSO) compared to a pharmaceutical composition (Formulation 3, comprising GO-HA + Compound 1) (Biological Example 3).

[0016] FIG. 8 shows inhibition of the Wnt transcription signaling pathway activity by a Wnt inhibitor of Formula (I) (Compound 7 in DMSO) compared to a pharmaceutical composition (Formulation 4, comprising GO-HA + Compound 7) (Biological Example 3).

[0017] FIG. 9 shows inhibition of the Wnt transcription signaling pathway activity by a Wnt inhibitor of Formula (I) (Compound 8 in DMSO) compared to a pharmaceutical composition (Formulation 5, comprising GO-HA + Compound 8) (Biological Example 3).

[0018] FIG. 10 illustrates the results of a rabbit ear study (Biological Example 5), comparing eight injury sites (LI, L2, L3, L4, R5, R6, R7, and R8) on three specimens at day 0 (top) and at day 23 (bottom). The specimens were treated with saline as a control, and a pharmaceutical composition (Formulation 14 with Compound 8 or Formulation 15 with Compound 18). Saline was applied via a spray, Formulation 14 (with Compound 8, 1 mg / mL) was applied via a phospholipid spray, and Formulation 15 (with Compound 18, 1 mg / mL) was applied via a phospholipid spray (Biological Example 5).

[0019] FIG. 11A shows rate of ear closure from days 1-21 (test 1). Saline control is compared to pharmaceutical compositions (Formulation 14 with Compound 8 and Formulation 15 with Compound 18) (Biological Example 5).

[0020] FIG. 11B shows rate of ear closure from days 14-39 (test 1). Saline control is compared to pharmaceutical compositions (Formulation 14 with Compound 8 and Formulation 15 with Compound 18) (Biological Example 5).

[0021] FIG. 12 shows rate of ear closure from days 14-39 (test 2). Saline control is compared to pharmaceutical compositions (Formulation 14 with Compound 8 and Formulation 15 with Compound 18) (Biological Example 5).

[0022] FIG. 13 (top) shows the location of an embed cut across the center point of a healed wound for purposes of processing tissue after cartilage regeneration tests (rabbit ear study, Biological Example 5). FIG. 13 (bottom) shows a slide with the cross section of sectioned tissue at the center of the wound. Resultant tissue sections placed on the slide are shown in images in FIGS. 15-17.

[0023] FIG. 14 shows the average distance between opposing cartilage endplates (mm) after 45 days in cartilage regeneration tests (rabbit ear studies). The measurements were taken from Safranin O stained cross sections of healed 8 mm ear punch wounds 45 days after treating with saline (control) or a pharmaceutical composition (Formulation 14 with Compound 8 or Formulation 15 with Compound 18).

[0024] FIG. 15 shows a representative sample of a cross section from the cartilage regeneration test (Biological Example 5) after treatment with saline (control), collected at day 45. Analysis is shown 9-10 mm from the wound margin. The top image includes the wound margin. Top image is at 0.3X magnification, middle image is at 2.5X magnification, and bottom image is at 10X magnification. The gray square at the middle image represents the image shown in the bottom image. Gray arrows in the bottom image show an area with regenerating cartilage.

[0025] FIG. 16 shows a representative sample of a cross section from the cartilage regeneration test (Biological Example 5) after treatment with a pharmaceutical composition (Formulation 14 with Compound 8), collected at day 45. Analysis is shown 9-10 mm from the wound margin. The top image includes the wound margin. Top image is at 0.3X magnification, middle image is at 2.5X magnification, and bottom image is at 10X magnification. The gray square at the middle image represents the image shown in the bottom image. Gray arrows in the bottom image show an area with regenerating cartilage.

[0026] FIG. 17 shows a representative sample of a cross section from the cartilage regeneration test (Biological Example 5) after treatment with a pharmaceutical composition (Formulation 15 with Compound 18), collected at day 45. Analysis is shown 9-10 mm from the wound margin. The top image includes the wound margin. Top image is at 0.3X magnification, middle image is at 2.5X magnification, and bottom image is at 10X magnification. The gray square at the middle image represents the image shown in the bottom image. Gray arrows in the bottom image show an area with regenerating cartilage.

[0027] FIG. 18 shows a schematic diagram of wound site locations on a mammal (swine) in a full thickness excisional wound healing study.

[0028] FIG. 19 (top) illustrates suture formation with a simple interrupted closure used in the full thickness excisional wound healing study (Biological Example 6), with the first throw 3401, the second throw 3403, and a portion of the wound closed by an interrupted suture 3405 shown. FIG. 19 (bottom) shows a cross section view of the portion of the wound closed by the interrupted suture 3405.

[0029] FIG. 20A shows results of the full thickness open excisional wound healing, rete ridge formation, with a pharmaceutical composition (Formulation 19 with Compound 8 + GO-HA), a saline control, and uninjured skin.

[0030] FIG. 20B shows results of the full thickness open excisional wound healing rete ridge formation (outliers removed), with pharmaceutical compositions (Formulation 16 with Compound 7, Formulation 17 with Compound 8, Formulation 18 with Compound 7 + GOHA, and Formulation 19 with Compound 8 + GO-HA), a saline control, and GO-HA alone (p*>0.05; p**>0.01; and p***>0.001).

[0031] FIG. 21 depicts results of the 3rd degree bum wound healing study (Biological Example 6) comparing a pharmaceutical composition (Formulation 22 with Compound 8) to a saline control. Wound area (cm2) was measured at 2-6 day intervals. Results from day 16 to day 30 are shown.

[0032] FIG. 22 depicts results of the 3rd degree bum wound healing study (Biological Example 6) comparing a pharmaceutical composition (Formulation 21 with Compound 7) to a saline control. Wound area (cm2) was measured at 2-6 day intervals. Results from day 16 to day 30 are shown.

[0033] FIG. 23 depicts results of the 3rd degree bum wound healing study (Biological Example 6) comparing a pharmaceutical composition (Formulation 26 with Compound 8 + GO-HA) to a saline control. Wound area (cm2) was measured at 2-6 day intervals. Results from day 16 to day 30 are shown.

[0034] FIG. 24 depicts results of the 3rd degree bum wound healing study (Biological Example 6) comparing a pharmaceutical composition (Formulation 25 with Compound 7 + GO-HA) to a saline control. Wound area (cm2) was measured at 2-6 day intervals. Results from day 16 to day 30 are shown.

[0035] FIG. 25 shows results of the closed excisional wound study (Biological Example 6): saline control, serum formulation (also called serum formulation control or serum control) as a vehicle, and GO-HA vehicle, from day 1 to day 21. The sutures were removed at day 13.

[0036] FIG. 26 shows results of the closed excisional wound study (Biological Example 6): pharmaceutical compositions (Formulation 20 with Compound 1, Formulation 22 with Compound 8, and Formulation 23 with Compound 10), from day 1 to day 21. The sutures were removed at day 13.

[0037] FIG. 27 shows results of the closed excisional wound study (Biological Example 6): pharmaceutical compositions (Formulation 24 with Compound 1 + GO-HA, Formulation 26 with Compound 8 + GO-HA, and Formulation 27 with Compound 10 + GOHA), from day 1 to day 21. The sutures were removed at day 13.

[0038] FIG. 28 shows results of the closed excisional wound study (Biological Example 6): cross sectional tissue samples stained with trichrome blue. Wound sites treated with saline control, serum formulation control, GO-HA vehicle, Formulations 20, 22, and 23 (with Compound 1, Compound 8, Compound 10, respectively), and Formulations 24, 26, and 27 (with Compound 1 + GO-HA, Compound 8 + GO-HA, and Compound 10 + GO-HA, respectively) are compared.

[0039] FIG. 29 shows results of the closed excisional wound study (Biological Example 6): cross sectional tissue samples under polarized microscopy. Wound sites treated with saline control, serum formulation control, GO-HA vehicle, Formulations 20, 22, and 23 (with Compound 1, Compound 8, Compound 10, respectively), and Formulations 24, 26, and 27 (with Compound 1 + GO-HA, Compound 8 + GO-HA, and Compound 10 + GO-HA, respectively) are compared.

[0040] FIG. 30 shows results of closed excisional wound polarized image collagen infiltration (Biological Example 6). The number of wounds indicating collagen infiltration in the scar are shown for A-saline (also called saline control); B-serum (also called serum formulation control); C-GO-HA vehicle (also called GO-HA control or GO-HA); D-(Formulation 20 with Compound 1); E-(Formulation 24 with Compound 1 + GO-HA); F-(Formulation 22 with Compound 8); G-(Formulation 26 with Compound 8 + GO-HA); H-(Formulation 23 with Compound 10); and J-(Formulation 27 with Compound 10 + GO-HA).

[0041] FIG. 31 shows results of the 3rd degree burn wound healing study (Biological Example 6) comparing a pharmaceutical composition (Formulation 21 with Compound 7, Formulation 25 with Compound 7 + GO-HA, Formulation 22 with Compound 8, and Formulation 26 with Compound 8 + GO-HA) to a saline control and GO-HA vehicle. Histology demonstrated that the pharmaceutical compositions promoted regeneration of tissue (improved organized reticular collagen and rete ridge formation) and reduced scar formation as compared to saline control and GO-HA vehicle.

[0042] FIG. 32 shows inhibition of the Wnt transcription signaling pathway activity by a compound of Formula (I) (Compound 1) compared to a compound of Formula (I) and GO-HA (GO-HA + Compound 1) (Biological Example 3).

[0043] FIG. 33 shows inhibition of the Wnt transcription signaling pathway activity by a compound of Formula (I) (Compound 7) compared to a compound of Formula (I) and GO-HA (GO-HA + Compound 7) (Biological Example 3).

[0044] FIG. 34 shows inhibition of the Wnt transcription signaling pathway activity by a compound of Formula (I) (Compound 8) compared to a compound of Formula (I) and GO-HA (GO-HA + Compound 8) (Biological Example 3). SUMMARY

[0045] Disclosed herein are pharmaceutical compositions comprising a WNT inhibitor, a glycol, one or more solubilizers, and saline or water. The pharmaceutical composition optionally comprises a graphene oxide-hyaluronic acid conjugate, one or more phospholipids, one or more carriers, stabilizer, buffer, and / or preservative. The pharmaceutical composition is suitable for topical administration.

[0046] In a first aspect, provided is a pharmaceutical composition comprising a WNT inhibitor which is 2-(4-(trifluoromethyl)phenyl)-l,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound A) and a graphene oxide-hyaluronic acid conjugate (GO-HA), wherein the pharmaceutical composition is a liposomal composition (in some embodiments a water-based liposomal composition), and preferably wherein the pharmaceutical composition comprises one or more phospholipids. Alternatively, the WNT inhibitor in the first aspect is according to the Compound of Formula (I) as defined in the fourth aspect or any embodiment thereof.

[0047] In a second aspect, provided is a pharmaceutical composition comprising a WNT inhibitor (in one or more embodiments, Compound A or a Compound of Formula (I) or any embodiments thereof), a graphene oxide-hyaluronic acid conjugate (GO-HA), a glycol, one or more solubilizers, one or more optional thickeners, one or more phospholipids, one or more carriers, an optional stabilizer, an optional buffer, an optional preservative, and saline or water. In one or more embodiments, provided is a pharmaceutical composition, comprising: a Wnt inhibitor which is 2-(4-(trifluoromethyl)phenyl)-l,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound A) preferably in an amount from about 0.01 wt% to about 2 wt%; a graphene oxide-hyaluronic acid conjugate (GO-HA), preferably in an amount from about 0.01 wt% to about 2 wt%; a glycol, preferably in an amount from about 2 wt% to about 10 wt%, or in an amount from about 3 wt% to about 6 wt%; one or more solubilizers, preferably in an amount from about 0.5 wt% to about 10 wt%, preferably about 1 wt% to about 6 wt%; one or more optional thickeners, and when present, preferably in an amount from about 0.5 wt% to about 2.5 wt%; one or more phospholipids, preferably in an amount from about 0.1 wt% to about 4 wt%; one or more carriers, preferably in an amount from about 2 wt% to about 10 wt%, preferably about 3 wt% to about 6 wt% when the one or more carrier is ethanol; an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%; an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%; an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; and saline or water; and wherein the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more optional thickeners (when present), one or more phospholipids, one or more carriers, optional stabilizer, (when present) optional buffer (when present), optional preservative (when present), and saline or water is 100 wt%; or wherein the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more phospholipids, one or more carriers, optional stabilizer, (when present) optional buffer (when present), optional preservative (when present), and saline or water is 100 wt%; wherein the wt% ratio of the WNT inhibitor to GO-HA is about 0.1:1 to about 12:1.

[0048] In a third aspect, provided is a pharmaceutical composition, comprising a Wnt inhibitor which is 2-(4-(trifluoromethyl)phenyl)-l,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound A) preferably in an amount from about 0.01 wt% to about 2 wt%; a graphene oxide-hyaluronic acid conjugate (GO-HA), preferably in an amount from about 0.01 wt% to about 2 wt%; a glycol, preferably in an amount from about 2 wt% to about 10 wt%, or in an amount from about 3 wt% to about 6 wt%; one or more solubilizers, preferably in an amount from about 0.5 wt% to about 10 wt%, preferably about 1 wt% to about 6 wt%; one or more optional thickeners, and when present, preferably in an amount from about 0.5 wt% to about 2.5 wt%; one or more phospholipids, preferably in an amount from about 0.1 wt% to about 4 wt%; one or more carriers, preferably in an amount from about 2 wt% to about 10 wt%, preferably about 3 wt% to about 6 wt% when the one or more carrier is ethanol; an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%; an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%; an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; and saline or water; and wherein the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more optional thickeners (when present), one or more phospholipids, one or more carriers, optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and saline or water is 100 wt%; or wherein the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more phospholipids, one or more carriers, optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and saline or water is 100 wt%; wherein the wt% ratio of the WNT inhibitor to GO-HA is about 0.1:1 to about 12:1; and wherein at least one of the stabilizer, buffer, and preservative is present; or wherein each of the stabilizer, buffer, and preservative is present. Alternatively, the WNT inhibitor in the third aspect is according to the Compound of Formula (I) as defined in the fourth aspect or any embodiment thereof.

[0049] In a fourth aspect, provided is provided herein is a pharmaceutical composition comprising: a WNT inhibitor of Formula (I), preferably in an amount from about 0.01 wt% to about 2 wt%; an optional graphene oxide-hyaluronic acid conjugate (GO-HA), and when present, preferably in an amount from about 0.01 wt% to about 2 wt%; a glycol, preferably in an amount from about 2 wt% to about 10 wt%, preferably about 3 wt% to about 6 wt%; one or more solubilizers, preferably in an amount from about 0.5 wt% to about 10 wt%, preferably about 1 wt% to about 6 wt%; one or more optional thickeners, and when present, preferably in an amount from about 0.5 wt% to about 2.5 wt%; one or more optional phospholipids, and when present, preferably in an amount from about 0.1 wt% to about 4 wt%; one or more optional carriers, and when present, preferably in an amount from about 2 wt% to about 10 wt% or in an amount from about 3 wt% to about 6 wt% when the one or more carrier is ethanol; an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%; an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%; an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; and saline or water; and wherein the wt% of the WNT inhibitor, optional GO-HA (when present), glycol, one or more solubilizers, one or more optional thickeners (when present), one or more optional phospholipids (when present), one or more optional carriers (when present), optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and water or saline is 100 wt%; wherein the wt% of the WNT inhibitor, optional GO-HA (when present), glycol, one or more solubilizers, one or more optional phospholipids (when present), one or more optional carriers (when present), optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and water or saline is 100 wt%; wherein the wt% ratio of the WNT inhibitor to GO-HA, when present, is about 0.1:1 to about 12:1; and wherein the WNT inhibitor of Formula (I) is according to: O I A l| J N R2 Ri Rla (I), or a single stereoisomer or mixture of stereoisomers thereof; a single tautomer or mixture of tautomers thereof; and / or a pharmaceutically acceptable salt thereof; wherein: R1 is hydrogen, deuterium, Ci-Csalkyl, -OH, -O-Ci-Csalkyl, -CH2OH, or -B(OH)2; Rla is hydrogen, deuterium, or Ci-Csalkyl; R2 is: (a) phenyl substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups; (b) phenyl substituted with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups and where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups; (c) phenyl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl-R3 where the phenyl in -phenyl-R3 is optionally substituted with 1, 2, or 3 R3a groups; (d) 5- or 6-membered heteroaryl substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups; (e) 5- or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl-R3 where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups; (f) 5- or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -(5- or 6-membered heteroaryl)-R3 where the 5-or 6-membered heteroaryl in -(5- or 6-membered heteroaryl)-R3 is optionally substituted with 1, 2, or 3 R3a groups; (g) Cs-Cecycloalkyl substituted with R3 and additionally optionally substituted with 1 or 2 R3a groups; (h) Cs-Cecycloalkyl substituted with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups, and where the Cs-Cecycloalkyl is optionally substituted with 1 or 2 R3a groups; (j) Cs-Cecycloalkyl substituted with phenyl where the phenyl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups, and where the cycloalkyl is optionally substituted with 1 or 2 R3a groups; (k) 3- to 8-membered heterocycloalkyl substituted with phenyl or substituted with 5- or 6-membered heteroaryl, where the phenyl and the 5- or 6-membered heteroaryl are substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups; or (m) -CH=CH-R5 where R5 is phenyl or 5- or 6- membered heteroaryl, where the phenyl and the 5- or 6-membered heteroaryl are substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups; R3 is independently selected from -B(0H)2, cyano, halo, halo-Ci-Cealkyl, -(Co-C6alkylene)-0-R4, or 5- to 10-membered heterocyclic wherein the 5- to 10-membered heterocyclic is optionally substituted with cyano; or when R2 is (a), then R3 and one R3a, when on adjacent carbons, together with the carbons to which they are attached form: OH / ■Q-, R?a R7a (a-1), where the * indicate the carbons shared with the phenyl ring and where the remaining optional R3a on the phenyl ring portion are as defined below, and each R7a is independently hydrogen or Ci-Cealkyl; each R3a is independently selected from cyano, halo, -OH, Ci-Cealkyl, halo-Ci-Cealkyl, and Ci-Cealkoxy; and R4 is hydroxy-Ci-Cealkyl, Ci-Cealkoxy-Ci-Cealkyl, or Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl.

[0050] In a fifth aspect, provided is a method of treating a disease, disorder, or condition associated with Wnt signaling pathway activity, comprising administering topically a pharmaceutical composition according to any one of the first, second, third, and fourth aspects, or any of one or more embodiments herein to a mammal in need thereof. DETAILED DESCRIPTION

[0051] The present disclosure provides for pharmaceutical compositions and methods of administration thereof to: induce healing of a wound (including a chronic wound, an acute wound, an alkali-burned corneal wound, and an incisional wound—open or closed), healing of a bum (including first, second, and third degree bums), or healing of a lesion (including lesions caused by HPV and / or a vims selected from the Poxviridae family of viruses); treat an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), a cartilage disease, a bone disease, organ fibrosis, or cancer (including melanoma and breast cancer); induce tissue regeneration (including but not limited to regeneration of damaged elastic cartilage); induce bacteriostasis; induce bacterial growth inhibition; maintain bacteriostasis; induce antifungal activity; induce neovascularization (in tissues in need thereof); induce reinnervation (of a de-nerved body part); inhibit osteoclast differentiation; enhance osteoblast differentiation; and / or inhibit bone destruction associate with breast cancer.

[0052] The wound may include but is not limited to one or more selected from the group consisting of a chronic wound, an acute wound, and alkali-burned corneal wound. The inflammatory dermatitis disease may include but is not limited to one or more selected from the group consisting of acne, psoriasis, rosacea, and scleroderma. The cartilage disease may include but is not limited to one or more selected from the group consisting of osteoarthritis, rheumatoid arthritis, internal derangement of the joints, and degenerative cartilage disease. The bone disease may include but is not limited to a bone disease with impaired bone formation, e.g., osteoporosis.

[0053] The organ fibrosis may include but is not limited to one or more selected from the group consisting of lung fibrosis, heart fibrosis, liver fibrosis, and kidney fibrosis.

[0054] The cancer may include but is not limited to melanoma, breast cancer, and / or prostate cancer.

[0055] Further provided herein are Wnt inhibitors, pharmaceutical compositions comprising the Wnt inhibitors, methods of preparing the Wnt inhibitors, and methods of using the Wnt inhibitors and pharmaceutical compositions in treating wounds, in particular, the enhanced tissue regeneration following treatment of a wound. Also provided herein are methods of treating conditions associated with Wnt transcription products or Wnt signaling pathway activity in a mammal comprising administering a therapeutically effective amount of a Wnt inhibitor or pharmaceutical composition to a mammal. In one or more embodiments, the mammal is a human. Definitions

[0056] When referring to the compounds provided herein, the following terms have the following meanings unless indicated otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art. In the event that there is a plurality of definitions for a term herein, those in this section prevail unless stated otherwise. Unless specified otherwise, where a term is defined as being substituted, the groups in the list of substituents are themselves unsubstituted. For example, a substituted alkyl group can be substituted, for example, with a cycloalkyl group, and the cycloalkyl group is not further substituted unless specified otherwise.

[0057] Reference to “about” a value or parameter herein includes (and describes) variations that are directed to that value or parameter per se. For example, description referring to “about X” includes description of “X”. As used herein, and unless otherwise specified, the terms “about” and “approximately,” when used in connection with temperatures, doses, amounts, or weight percent of ingredients of a composition or a dosage form, mean a dose, amount, or weight percent that is recognized by those of ordinary skill in the art to provide a pharmacological effect equivalent to that obtained from the specified dose, amount, or weight percent. Specifically, the terms “about” and “approximately,” when used in this context, contemplate a dose, amount, or weight percent within 15%, within 10%, within 5%, within 4%, within 3%, within 2%, within 1%, or within 0.5% of the specified dose, amount, or weight percent.

[0058] The terms “a” or “an,” as used in herein means one or more, unless context clearly dictates otherwise. For example, “pharmaceutically acceptable carrier” includes one or more ingredients as provided herein.

[0059] It is to be understood that the description of compounds, compositions, formulations, and methods of treatment described herein include “comprising,” “consisting of,” and “consisting essentially of’ some or any embodiments. In one or more embodiments, for all compositions described herein, and all methods using a composition described herein, the compositions can either comprise the listed components or steps or can “consist essentially of’ the listed components or steps. When a composition is described as “consisting essentially of’ the listed components, the composition contains the components listed, and may contain other components which do not substantially affect the basic and novel properties (in one or more embodiments, the condition being treated), but do not contain any other components which substantially affect the basic and novel properties (in one or more embodiments, condition being treated) other than those components expressly listed; or, if the composition does contain extra components other than those listed which substantially affect the basic and novel properties (in one or more embodiments, the condition being treated), the composition does not contain a sufficient concentration or amount of the extra components to substantially affect the basic and novel properties (in one or more embodiments, the condition being treated). When a method is described as “consisting essentially of’ the listed steps, the method contains the steps listed, and may contain other steps that do not substantially affect the basic and novel properties (in one or more embodiments, the condition being treated), but the method does not contain any other steps which substantially affect the basic and novel properties (in one or more embodiments, the condition being treated) other than those steps expressly listed. As a nonlimiting specific example, when a composition is described as “consisting essentially of’ a component, the composition may additionally contain any amount of pharmaceutically acceptable carriers, vehicles, or diluents and other such components which do not substantially affect the basic and novel properties (in one or more embodiments, the condition being treated).

[0060] As used herein for GO-HA linker “alkyl” refers to straight or branched hydrocarbon. An alkyl may be linear, branched, cyclic, or a combination thereof, and may contain, for example, from one to sixty carbon atoms, in one or more embodiments, 2-25 carbons. Examples of alkyl groups include but are not limited to ethyl, ethyl, propyl, isopropyl, cyclopropyl, butyl isomers (e.g. n-butyl, iso-butyl, tert-butyl, etc.) cyclobutyl isomers (e.g. cyclobutyl, methylcyclopropyl, etc.), pentyl isomers, cyclopentyl isomers, hexyl isomers, cyclohexyl isomers, and the like.

[0061] The term “alkyl,” as used herein for a Wnt inhibitor of Formula (I), unless otherwise specified, refers to a saturated straight or branched, monovalent hydrocarbon. In one or more embodiments, the alkyl group is a primary, secondary, or tertiary hydrocarbon. An alkyl may be linear or branched, and may contain, for example, from one to eight carbon atoms. In one or more embodiments, the alkyl group has one to six carbon atoms, i.e., Ci to Ce alkyl (Ci-Cealkyl). In one or more embodiments, the alkyl is a Ci-Csalkyl. In one or more embodiments, the alkyl group is selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, isobutyl, secbutyl, / -butyl, pentyl, isopentyl, neopentyl, hexyl, isohexyl, 3-methylpentyl, 2,2-dimethylbutyl, and 2,3-dimethylbutyl. Examples of alkyl groups include but are not limited to methyl, ethyl, propyl, isopropyl, cyclopropyl, butyl isomers (e.g. n-butyl, iso-butyl, tert-butyl, etc.) pentyl isomers, hexyl isomers, and the like.

[0062] As used herein for the GO-HA linker, the term “linear alkyl” refers to a chain of carbon and hydrogen atoms (e.g., ethane, propane, butane, pentane, hexane, etc.).

[0063] As used herein for the GO-HA linker, the term “branched alkyl” refers to a chain of carbon and hydrogen atoms, without double or triple bonds that contains a fork, branch, and / or split in the chain. “Branching” refers to the divergence of a carbon chain, whereas “substitution” refers to the presence of non-carbon / non-hydrogen atoms in a moiety.

[0064] The term “alkylene,” as used herein, unless otherwise specified, refers to a divalent alkyl group, as defined herein for either the GO-HA linker or as defined for a Wnt inhibitor of formula (I), as applicable.

[0065] As used herein for the GO-HA linker, the term “cycloalkyl” refers to a completely saturated mono- or multi-cyclic hydrocarbon ring system. When composed of two or more rings, the rings may be joined together in a fused, bridged or spiro-connected fashion. A cycloalkyl group may be unsubstituted, substituted, branched, and / or unbranched. Typical cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like. If substituted, the substituent(s) may be an alkyl (but not substituted alkyl) or selected from those indicated above with regard to substitution of an alkyl group unless otherwise indicated. Unless specified otherwise (e.g., substituted cycloalkyl group, heterocyclyl, cycloalkoxy group, halocycloalkyl, cycloalkylamine, thiocycloalkyl, etc.), an alkyl group contains carbon and hydrogen atoms exclusively. In some or any embodiments, the cycloalkyl group includes three to six carbon atoms, i.e., C3 to Ce cycloalkyl. In some or any embodiments, the cycloalkyl has 3, 4, 5, or 6 (C3-6) 3, 4, or 5 (C3-5); 3 or 4 (C3-4); 3 (C3); 4 (C4); or 5 (C5) carbon atoms. In some or any embodiments, the cycloalkyl group is cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. In some or any embodiments, the cycloalkyl group is cyclopropyl, cyclobutyl, or cyclopentyl. In one or more embodiments, the cycloalkyl group is cyclobutyl.

[0066] The term “Cs-Cio-cycloalkyl,” as used herein for the Wnt inhibitor of Formula (I), refers to a monovalent, saturated, monocyclic hydrocarbon or bicyclic (fused, bridged, or spirocyclic) ring. In some or any embodiments, the terms “fused cycloalkyl” and “spirocycloalkyl” are embodiments of the cycloalkyl group. In some or any embodiments, the cycloalkyl group includes three to six carbon atoms, i.e., C3 to Ce cycloalkyl (“Cs-Ce-cycloalkyl”). In some or any embodiments, the cycloalkyl has 3, 4, or 5 (C3-5); 3 or 4 (C3-4); 3 (C3); 4 (C4); or 5 (C5) carbon atoms. In some or any embodiments, the cycloalkyl group is cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. In some or any embodiments, the cycloalkyl group is cyclopropyl, cyclobutyl, or cyclopentyl. In some or any embodiments, the cycloalkyl group is cyclopropyl. In some or any embodiments, the cycloalkyl group is cyclobutyl. In some or any embodiments, the cycloalkyl group is cyclopentyl. In some or any embodiments, the cycloalkyl group is bicyclo[3.2.1]octyl, bicyclo[2.2.2]octyl, bicyclo[3.3.1]nonyl, bicyclo[3.3.2.]decyl, bicyclo[2.2.2]octyl, or adamantyl. In one or more embodiments, the cycloalkyl group is cyclobutyl.

[0067] The term “alkoxy” and “alkyloxy,” as used herein, and unless otherwise specified, refer to the group -OR' where R' is alkyl. In one or more embodiments, alkoxy is Ci-Cealkoxy. In one or more embodiments, alkoxy is Ci-Csalkoxy, such as -O-Ci-Csalkyl. Alkoxy and alkyloxy groups include, in one or more embodiments, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, tert-butoxy, sec-butoxy, n-pentoxy, n-hexyloxy, 1,2-dimethylbutoxy, and the like.

[0068] The term “alkoxyalkyl,” as used herein for a Wnt inhibitor of Formula (I), and unless otherwise specified, refers to an alkyl group, as defined herein, substituted with one or two -OR' groups where each R' is alkyl, as defined herein, and is independently selected. In some or any embodiments, alkoxyalkyl is Ci-Cealkoxy-Ci-Cealkyl.

[0069] The term “ alkoxy carbonyl-NH-alkyl,” as used herein for a Wnt inhibitor of Formula (I), and unless otherwise specified, refers to an alkyl group substituted with -NH-C(O)O(alkyl), wherein alkyl is as defined herein. In some or any embodiments, alkoxycarbonyl-NH-alkyl is Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl.

[0070] As used herein “alkenyl,” as used herein for the GO-HA linker, means a straight or branched chain hydrocarbon having at least 2 carbon atoms, which contains at least one carboncarbon double bond.

[0071] As used herein “alkynyl,” as used herein for the GO-HA linker, means a straight or branched chain hydrocarbon having at least 2 carbon atoms, which contains at least one carboncarbon triple bond.

[0072] As used herein “amine” or “amino” as used herein for the GO-HA linker are represented by a formula -NA1A2, where Ai and A2 are, independently, hydrogen or alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, cycloalkynyl, aryl, or heteroaryl group where each of these groups are as described herein for the GO-HA linker. In one or more embodiments amine (or amino) refers to any of NH2, NH(alkyl), NH(aryl), N(alkyl)2, N(alkyl)(aryl), and N(aryl)2.

[0073] The term “aryl,” as used herein, and unless otherwise specified, refers to a monovalent Ce- C15 carbocyclic ring system which comprises at least one aromatic ring wherein the aryl ring system is mono, di, or tricyclic. The aryl may be attached to the main structure through any of its rings, i.e. any aromatic or nonaromatic ring. In some or any embodiments, the aryl group may be a bridged (where chemically feasible) or non-bridged, spirocyclic (where chemically feasible) or not spirocyclic, and / or fused or not fused multicyclic group. In some or any embodiments, aryl is Ce-Cio aryl. In some or any embodiments, aryl is Ce aryl, i.e. phenyl. In some or any embodiments, aryl is phenyl, naphthyl, indanyl, fluorenyl, 6,7,8,9-tetrahydro-5J / -benzo[7]annulenyl, or tetrahydronaphthyl. When aryl is substituted, it can be substituted on any ring, i.e. on any aromatic or nonaromatic ring comprised by aryl.

[0074] The term “haloalkyl,” as used herein, and unless otherwise specified, refers to an alkyl group substituted with 1, 2, 3, 4, or 5 halo groups. In some or any embodiments, the haloalkyl is a halo-Ci-Cealkyl. In some or any embodiments, the haloalkyl is -CF3, -CH2F, -CHF2, or -CH2CF3.

[0075] The terms “halogen” and “halo,” as used herein, and unless otherwise specified, are synonymous and refer to chloro, bromo, fluoro, or iodo.

[0076] The term “heteroaryl,” as used herein, and unless otherwise specified, refers to a monocyclic aromatic ring system or multicyclic aromatic ring system wherein one or more (in some or any embodiments, 1, 2, 3, or 4) of the ring atoms is a heteroatom independently selected from O, S(0)o-2, NH, and N, and the remaining ring atoms are carbon atoms, and where the ring may be optionally substituted as described herein. The heteroaryl group is bonded to the rest of the molecule through any atom in the ring system, valency rules permitting. In some or any embodiments, each ring of a heteroaryl group can contain one or two O atoms, one or two S atoms, and / or one to four N atoms, or a combination thereof, provided that the total number of heteroatoms in each ring is four or less and each ring contains at least one carbon atom. In some or any embodiments, the heteroaryl has from 5 to 20, from 5 to 15, from 5 to 6 ring atoms, or from 5 to 10 ring atoms. When heteroaryl is substituted, it can be substituted on any ring. In one or more embodiments, heteroaryl is a 5- to 10-membered heteroaryl. In one or more embodiments, heteroaryl is a 5- or 6-membered heteroaryl. In one or more embodiments, / =N heteroaryl is a 6-membered heteroaryl. In one or more embodiments, heteroaryl is hO   K ”> hO x—y ,     v—7 , N , or N , wherein indicates the point of attachment of the heteroaryl to the rest of the molecule.

[0077] In some or any embodiments, monocyclic heteroaryl groups include, but are not limited to, furanyl, imidazolyl, isothiazolyl, isoxazolyl, oxadiazolyl, oxadiazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridyl, pyrimidinyl, pyrrolyl, thiadiazolyl, thiazolyl, thienyl, tetrazolyl, triazinyl and triazolyl. In some or any embodiments, bicyclic heteroaryl groups include, but are not limited to, benzofuranyl, benzimidazolyl, benzoxazolyl, benzoisoxazolyl, benzopyranyl, benzothiadiazolyl, benzothiazolyl, benzoisothiazolyl, benzothienyl, benzotriazolyl, furopyridyl, thienopyridyl, imidazopyridinyl, imidazothiazolyl, indolizinyl, indolyl, indazolyl, isobenzofuranyl, isobenzothienyl, isoindolyl, isoquinolinyl, isothiazolyl, naphthyridinyl, oxazolopyridinyl, phthalazinyl, pteridinyl, purinyl, pyridopyridyl, pyrrolopyridyl, quinolinyl, quinoxalinyl, or quinazolinyl. In some or any embodiments, tricyclic heteroaryl groups include, but are not limited to, acridinyl, benzindolyl, carbazolyl, dibenzofuranyl, perimidinyl, phenanthrolinyl, phenanthridinyl, and phenazinyl. In some or any embodiments, heteroaryl is indolyl, furanyl, pyridinyl, pyrimidinyl, imidazolyl, or pyrazolyl; each of which is optionally substituted with 1, 2, 3, or 4 groups as defined throughout the specification, including in one or more embodiments with group(s) independently selected from Ci-Cealkyl, hydroxy, halo, halo-Ci-Cealkyl, Ci-Cealkoxy, cyano, or phenyl.

[0078] The term “heterocyclic,” as used herein, and unless otherwise specified, refers to a monovalent monocyclic non-aromatic ring system or a monovalent multicyclic ring system that contains at least one non-aromatic ring; wherein one or more (in some or any embodiments, 1, 2, 3, or 4) of the monocyclic non-aromatic ring atoms is a heteroatom independently selected from O, S(0)o-2, and N, and the remaining ring atoms are carbon atoms; and wherein one or more (in some or any embodiments, 1, 2, 3, or 4) of any of the ring atoms in the multicyclic ring system is a heteroatom(s) independently selected from O, S(0)o-2, and N, and the remaining ring atoms are carbon. The term “heterocyclic” does not include fully aromatic ring(s), i.e. does not include imidazole, pyrimidine, pyridine, and the like. In some or any embodiments, the heterocyclic ring comprises one or two heteroatom(s) which are independently selected from nitrogen and oxygen. In some or any embodiments, the heterocyclic ring comprises one or two heteroatom(s) which are oxygen. In some or any embodiments, the heterocyclic ring comprises one or two heteroatom(s) which are nitrogen (where the nitrogen is substituted as described in any aspect or embodiment described herein). In some or any embodiments, heterocyclic is multicyclic and comprises one heteroatom in a non-aromatic ring, or comprises one heteroatom in an aromatic ring, or comprises two heteroatoms in an aromatic ring, or comprises two heteroatoms where one is in an aromatic ring and the other is in a non-aromatic ring. In some or any embodiments, the heterocyclic group has from 3 to 20, 3 to 15, 3 to 10, 3 to 8, 4 to 7, or 5 to 6 ring atoms. In one or more embodiments, the heterocyclic is a 4- to 10-membered heterocyclic. In one or more embodiments, the heterocyclic is a 5- to 10-membered heterocyclic. In some or any embodiments, the heterocyclic is a monocyclic, bicyclic, tricyclic, or tetracyclic ring system. In some or any embodiments, the heterocyclic group may be a bridged or non-bridged, spirocyclic or not spirocyclic, and / or fused or not fused multicyclic group. One or more of the nitrogen and sulfur atoms may be optionally oxidized, one or more of the nitrogen atoms may be optionally quaternized, one or more of the carbon atoms may be optionally replaced with 0 . Some rings may be partially or fully saturated, or aromatic provided that heterocyclic is not fully aromatic. The monocyclic and multicyclic heterocyclic rings may be attached to the main structure at any heteroatom or carbon atom which results in a stable compound. The multicyclic heterocyclic may be attached to the main structure through any of its rings, including any aromatic or nonaromatic ring, regardless of whether the ring contains a heteroatom. In some or any embodiments, heterocyclic is “heterocycloalkyl” which is 1) a saturated monovalent monocyclic group which contains at least one ring heteroatom, as described herein, or 2) a saturated monovalent bi- or tri-cyclic group in which at least one ring contains at least one heteroatom as described herein. In some or any embodiments, heterocyclic is 3- to 6-membered heterocycloalkyl. In some or any embodiments, heterocyclic is 3- to 8-membered heterocycloalkyl. In some or any embodiments, heterocyclic is 3- to 9- membered heterocycloalkyl. When heterocyclic and heterocycloalkyl are substituted, they can be substituted on any ring, i.e. on any aromatic or nonaromatic ring comprised by heterocyclic and heterocycloalkyl. In some or any embodiments, such heterocyclic includes, but are not limited to, azepinyl, benzodioxanyl, benzodioxolyl, 3,4-dihydro-2H-benzo[b][l,4]oxazinyl, 3,4-dihydro-2H-benzo[b][l,4]dioxepinyl, 1,3-dihydroisobenzofuranyl, benzofuranonyl, benzopyranonyl, benzopyranyl, dihydrobenzofuranyl, benzotetrahydrothienyl, 2,2-dioxo-l,3-dihydrobenzo[c]thienyl, benzothiopyranyl, benzoxazinyl, P-carbolinyl, chromanyl, chromonyl, cinnolinyl, coumarinyl, decahydroquinolinyl, decahydroisoquinolinyl, dihydro-benzimidazolonyl (including but not limited to 2-oxo-l,3-dihydro-2H-benzo[d]imidazol-l-yl), dihydrobenzisothiazinyl, dihydrobenzisoxazinyl, dihydrofuryl, dihydroisoindolyl, dihydropyranyl, dihydropyrazolyl, dihydropyrazinyl, dihydropyridinyl, dihydropyrimidinyl, dihydropyrrolyl, dioxolanyl, 1,4-dithianyl, furanonyl, imidazolidinyl, 2,4-dioxo-imidazolidinyl, imidazolinyl, indolinyl, 2-oxo-indolinyl, isobenzotetrahydrofuranyl, isobenzotetrahydrothienyl, isochromanyl, isocoumarinyl, isoindolinyl, 1-oxo-isoindolinyl, 1,3-dioxo-isoindolinyl, isothiazolidinyl, isoxazolidinyl, 3-oxo-isoxazolidinyl, morpholinyl, 3,5-dioxo-morpholinyl, octahydroindolyl, octahydroisoindolyl, 1-oxo-octahydroisoindolyl, 1,3-dioxo-hexahydroisoindolyl, oxo-oxadiazolyl (including but not limited to 5-oxo-l,2,4-oxadiazol-3-yl), oxazolidinonyl, oxazolidinyl, oxiranyl, piperazinyl, 2,6-dioxo-piperazinyl, piperidinyl, 2,6-dioxo-piperidinyl, 4-piperidonyl, pyrazolidinyl, pyrazolinyl, pyrrolidinyl, pyrrolinyl, 2-oxopyrrolidinyl, 2,5-dioxopyrrolidinyl, quinuclidinyl, tetrahydrofuryl, tetrahydroisoquinolinyl, tetrahydropyranyl, tetrahydrothienyl, thiamorpholinyl, thiomorpholinyl, 3,5-dioxo-thiomorpholinyl, thiazolidinyl, 2,4-dioxo-thiazolidinyl, tetrahydroquinolinyl, phenothiazinyl, phenoxazinyl, xanthenyl, 1,3,5-trithianyl, or l,3-dihydro-imidazopyridin-2-onyl. In some or any embodiments, heterocyclic is benzo-1,4-dioxanyl, benzodi oxolyl, indolinyl, 2-oxo-indolinyl, pyrrolidinyl, piperidinyl, 2,3-dihydrobenzofuranyl,          decahydroquinolinyl,          dihydrocyclopentapyridyl, dihydropyranopyridyl, tetrahydronaphthyridyl, 2,2-dioxo-3,4-dihydro-thiopyrano-pyridyl, dihydrofuropyridyl, dihydropyrrolopyridyl, 2,2-di oxo-1,3-dihydro-thi eno-pyridyl, or tetrahydrocyclopropacyclopentapyridyl, ; each of which is optionally substituted with 1, 2, 3, or 4 groups as defined throughout the specification, including in some or any embodiments with group(s) independently selected from halo, alkyl, and phenyl. In some or any embodiments, heterocycloalkyl is pyrrolidinyl. In some or any embodiments, heterocycloalkyl is an N-linked heterocycloalkyl.

[0079] The term “hydroxyalkyl” as used herein, unless otherwise specified, refers to an alkyl, as defined herein, substituted by 1, 2, or 3 hydroxy groups. In one or more embodiments, the hydroxy group is a primary, secondary, or tertiary alcohol. In one or more embodiments, the hydroxyalkyl group includes one to ten carbons, ie., Ci to Cio hydroxyalkyl. In one or more embodiments, the hydroxyalkyl group includes one or two alcohol (hydroxy) groups, provided that they are not on the same carbon. In one or more embodiments, the hydroxyalkyl group is hydroxyCi-Cealkyl. In one or more embodiments, the hydroxyalkyl group is hydroxyCi-Csalkyl. In one or more embodiments, the hydroxyalkyl group is selected from the group consisting of hydroxymethyl, hydroxy ethyl, hydroxypropyl, hydroxybutyl, hydroxypentyl, and hydroxyhexyl. In one or more embodiments, the hydroxyalkyl group is a Ci-Cehydroxyalkyl. In one or more embodiments, the hydroxyalkyl group is selected from the group consisting of hydroxymethyl (-CH2OH), 1-hydroxyethyl, 2-hydroxy ethyl, l-hydroxypropan-2-yl, and 2-hy droxypropan-2-yl.

[0080] The term “oxo” as used herein and unless otherwise specified, refers to a keto group (C=O). An oxo group that is a substituent of a nonaromatic carbon results in a conversion of a -CH2- to -C=O. An oxo group that is a substituent of an aromatic carbon results in a conversion of -CH- to -C=O. When a substituent is oxo, then two hydrogens on the atom are replaced. When an oxo group substitutes aromatic moieties, the corresponding partially unsaturated ring replaces the aromatic ring. For example, a pyridyl group substituted by an oxo group is a pyridone. The person of ordinary skill in the art will appreciate that in one or more embodiments that such a group, e.g. pyridone and 2,4(U / ,3J7)-dioxo-pyrimidinyl, can exist in its tautomeric form, e.g. hydroxypyridine and 2,4-dihydroxypyrimidinyl, respectively.

[0081] The term “oxo” as used herein and unless otherwise specified, refers to a keto group (C=O). An oxo group that is a substituent of a nonaromatic carbon results in a conversion of a -CH2- to -C=O. An oxo group that is a substituent of an aromatic carbon results in a conversion of -CH- to -C=O. When a substituent is oxo, then two hydrogens on the atom are replaced. When an oxo group substitutes aromatic moieties, the corresponding partially unsaturated ring replaces the aromatic ring. For example, a pyridyl group substituted by an oxo group is a pyridone. The person of ordinary skill in the art will appreciate that in some embodiments that such a group, e.g. pyridone and 2,4(U / ,3J7)-dioxo-pyrimidinyl, can exist in its tautomeric form, e.g. hydroxypyridine and 2,4-dihydroxypyrimidinyl, respectively.

[0082] As used herein “regeneration” means the renewal or growth of destroyed or devitalized tissue from the remnant tissue. It is a reparative attempt of the body, and in the context of wound represents the migration, differentiation, or replication of cells or transformation of progenitor cells into the appropriate cell types for the respective tissue which may include sebaceous cells, hair follicles, nerve cells, chondrocytes.

[0083] As used herein “wound” means an injury to tissue or skin caused by scrapes, cuts, abrasion, surgical procedures (e.g., caused by minimally invasive surgery, laparoscopic surgery, robotic surgery, incisional biopsies, general surgery, and cosmetic surgery), denuded skin, bums, ulcers (e.g., diabetic ulcers, ulcers from vascular insufficiency, pressure sores, and burns), or other skin problems (e.g., allergies). Wound may range from superficial (e.g., affecting merely the epidermis) to more traumatic (e.g., lesions which affect layers of skin or tissue at depths which are beneath the epidermis). Wounds may be of any length or shape, e.g., in one or more embodiments, wounds are straight, jagged or curved.

[0084] In one or more embodiments, the term “pharmaceutically acceptable carrier” includes any and all and / or one or more solvents, co-solvents, complexing agents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, excipients, diluents, disintegrants, lubricants, adjuvants, and the like which are not biologically or otherwise undesirable. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incorporated into the compositions. In addition, various adjuvants such as are commonly used in the art may be included. These and other such compounds are described in the literature, e.g., in the Merck Index (Merck & Company, Rahway, N.J.), considerations for the inclusion of various components in pharmaceutical compositions are described, (e.g., Gilman et al. (Eds.), 2010, Goodman and Gilman’s: The Pharmacological Basis of Therapeutics, 12th Ed., The McGraw-Hill Companies).

[0085] In one or more embodiments, the term “pharmaceutically acceptable salt” refers to salts that retain the biological effectiveness and properties of the compounds provided herein and which are not biologically or otherwise undesirable. In many cases, the compounds provided herein are capable of forming acid and / or base salts by virtue of the presence of amino and / or carboxyl groups or groups similar thereto. Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and organic acids. Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like. Organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like. Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases. Inorganic bases from which salts can be derived include, for example, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum, and the like. Of these, the inorganic bases from which salts can be derived can include ammonium, potassium, sodium, calcium and magnesium salts. Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, basic ion exchange resins, and the like, specifically such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine. Many such salts are known in the art, as described in WO 87 / 05297.

[0086] In one or more embodiments, the term “pharmaceutically acceptable salt,” as used herein, and unless otherwise specified, refers to any salt of a compound provided herein which retains its biological properties and which is not toxic or otherwise desirable for pharmaceutical use. Such salts may be derived from a variety of organic and inorganic counter-ions well known in the art. Such salts include, but are not limited to: (1) acid addition salts formed with organic or inorganic acids such as hydrochloric, hydrobromic, sulfuric, nitric, phosphoric, sulfamic, acetic, trifluoroacetic, trichloroacetic, propionic, hexanoic, cyclopentylpropionic, glycolic, glutaric, pyruvic, lactic, malonic, succinic, sorbic, ascorbic, malic, maleic, fumaric, tartaric, citric, benzoic, 3-(4-hydroxybenzoyl)benzoic, picric, cinnamic, mandelic, phthalic, lauric, methanesulfonic, ethanesulfonic, 1,2-ethane-disulfonic, 2-hydroxy ethanesulfonic, benzenesulfonic, 4-chlorobenzenesulfonic, 2-naphthalenesulfonic, 4-toluenesulfonic, camphoric, camphorsulfonic, 4-methylbicyclo[2.2.2]-oct-2-ene-l-carboxylic, glucoheptonic, 3-phenylpropionic, trimethylacetic, tert-butyl acetic, lauryl sulfuric, gluconic, benzoic, glutamic, hydroxynaphthoic, salicylic, stearic, cyclohexylsulfamic, quinic, muconic acid and the like acids; and (2) base addition salts formed when an acidic proton present in the parent compound either (a) is replaced by a metal ion, e.g., an alkali metal ion, an alkaline earth ion or an aluminum ion, or alkali metal or alkaline earth metal hydroxides, such as sodium, potassium, calcium, magnesium, aluminum, lithium, zinc, and barium hydroxide, ammonia or (b) coordinates with an organic base, such as aliphatic, alicyclic, or aromatic organic amines, such as ammonia, methylamine, dimethylamine, diethylamine, picoline, ethanolamine, diethanolamine, triethanolamine, ethylenediamine, lysine, arginine, ornithine, choline, N,N'-dibenzylethylene-diamine, chloroprocaine, diethanolamine, procaine, N-benzylphenethylamine, 7V-methylglucamine tris(hydroxymethyl)-aminomethane, piperazine, tetramethylammonium hydroxide, and the like.

[0087] In one or more embodiments, pharmaceutically acceptable salts further include, in some or any embodiments, and without limitation, sodium, potassium, calcium, magnesium, ammonium, tetraalkylammonium salts and the like. When the compound contains a basic functionality, salts of non-toxic organic or inorganic acids, such as hydrohalides, e.g. hydrochloride and hydrobromide, sulfate, phosphate, sulfamate, nitrate, acetate, trifluoroacetate, tri chloroacetate, propionate, hexanoate, cyclopentylpropionate, glycolate, glutarate, pyruvate, lactate, malonate, succinate, sorbate, ascorbate, malate, maleate, fumarate, tartarate, citrate, benzoate, 3-(4-hydroxybenzoyl)benzoate, picrate, cinnamate, mandelate, phthalate, laurate, methanesulfonate (mesylate), ethanesulfonate, 1,2-ethane-disulfonate, 2-hydroxyethanesulfonate, benzenesulfonate (besylate), 4-chlorobenzenesulfonate, 2-naphthalenesulfonate, 4-toluenesulfonate, camphorate, camphorsulfonate, 4-methylbicyclo[2.2.2]-oct-2-ene-l-carboxylate, glucoheptonate, 3-phenylpropionate, trimethylacetate, tert-butyl acetate, lauryl sulfate, gluconate, benzoate, glutamate, hydroxynaphthoate, salicylate, stearate, cyclohexylsulfamate, quinate, muconate and the like.

[0088] In one or more embodiments, a “therapeutically effective amount” or “pharmaceutically effective amount” of a compound as provided herein, is one which is sufficient to achieve the desired effect and may vary according to the nature and severity of the disease condition, and the potency of the compound. “Therapeutically effective amount” is also intended to include one or more of the compositions of the present disclosure so as to result in the increased regeneration of tissue subject to a wound. The combination of compounds is preferably a synergistic combination. Synergy, as described in the art (for example, Chou, 2010, Cane. Res. 70(2):440-446), occurs when the effect of the compounds when administered in combination is greater than the additive effect of the compounds when administered alone as a single agent. In general, a synergistic effect is most clearly demonstrated at sub-optimal concentrations of the compounds. This amount can further depend upon the patient's height, weight, sex, age and medical history.

[0089] The term “mammal” specifically includes humans, cattle, horses, dogs, and cats, but also includes many other mammalian species like pigs, rats, mice, primates (e.g., a monkey such as a cynomolgous monkey, a chimpanzee and a human). In one or more embodiments, the mammal is a human.

[0090] The term “subject” refers to a mammal, as provided herein, as well as to a cell or biological sample.

[0091] The term “substantially free of’ stereoisomers with respect to a composition refers to a composition that includes at least 85% or 90% by weight, in some or any embodiments 95%, 98%, 99% or 100% by weight, of a designated stereoisomer of a compound in the composition. In some or any embodiments, in the methods and compounds provided herein, the compounds are substantially free of stereoisomers.

[0092] Similarly, the term “isolated” with respect to a composition refers to a composition that includes at least 85%, 90%, 95%, 98%, 99% to 100% by weight, of a specified compound, the remainder comprising other chemical species or stereoisomers.

[0093] The term “isotopic composition,” as used herein, and unless otherwise specified, refers to the amount of each isotope present for a given atom, and “natural isotopic composition” refers to the naturally occurring isotopic composition or abundance for a given atom. Atoms containing their natural isotopic composition may also be referred to herein as “non-enriched” atoms. Unless otherwise designated, the atoms of the compounds recited herein are meant to represent any stable isotope of that atom. For example, unless otherwise stated, when a position is designated specifically as “H” or “hydrogen,” the position is understood to have hydrogen at its natural isotopic composition. An isotopic composition may be included in a pharmaceutical composition disclosed herein.

[0094] The term “isotopic enrichment,” as used herein, and unless otherwise specified, refers to the percentage of incorporation of an amount of a specific isotope at a given atom in a molecule in the place of that atom’s natural isotopic abundance. In some or any embodiments, deuterium enrichment of 1% at a given position means that 1% of the molecules in a given sample contain deuterium at the specified position. Because the naturally occurring distribution of deuterium is about 0.0156%, deuterium enrichment at any position in a compound synthesized using non-enriched starting materials is about 0.0156%. The isotopic enrichment of the compounds provided herein can be determined using conventional analytical methods known to one of ordinary skill in the art, including mass spectrometry and nuclear magnetic resonance spectroscopy.

[0095] The term “isotopically enriched,” as used herein, and unless otherwise specified, refers to an atom having an isotopic composition other than the natural isotopic composition of that atom. “Isotopically enriched” may also refer to a compound containing at least one atom having an isotopic composition other than the natural isotopic composition of that atom.

[0096] As used herein, and unless otherwise specified, the term “IC50” refers to an amount, concentration or dosage of a particular test compound that achieves a 50% inhibition of a maximal response in an assay that measures such response.

[0097] In one or more embodiments, the terms “therapeutic agent” and “therapeutic agents” refer to any agent(s) which can be used in the treatment or prevention of a disorder or one or more symptoms thereof. In some or any embodiments, the term “therapeutic agent” includes a compound provided herein. In some or any embodiments, a therapeutic agent is an agent which is known to be useful for, or has been or is currently being used for the treatment or prevention of a disorder or one or more symptoms thereof.

[0098] In one or more embodiments, “treating” or “treatment” of any condition or disorder refers, in some or any embodiments, to ameliorating a condition or disorder that exists in a subject. In another embodiment, “treating” or “treatment” includes ameliorating one or more physical parameter, which may be indiscernible by the subject. In yet another embodiment, “treating” or “treatment” includes modulating the condition or disorder, either physically (e.g., stabilization of a discernible symptom) or physiologically (e.g., stabilization of a physical parameter) or both. In yet another embodiment, “treating” or “treatment” includes delaying the onset of the condition or disorder. In yet another embodiment, “treating” or “treatment” includes the reduction or elimination of either the condition or one or more symptoms of the condition, or to retard the progression of the condition or of one or more symptoms of the condition, or to reduce the severity of the condition or of one or more symptoms of the condition. Pharmaceutical Composition

[0099] Provided herein are compositions that can induce improved wound healing and tissue regeneration. Provided herein are compositions that can treat wounds and / or conditions associated with Wnt transcription products or Wnt signaling pathway activity, and in particular enhance tissue regeneration following treatment of a wound. In one or more embodiments, the composition is a pharmaceutical composition.

[00100] Further provided herein are compositions that can modulate the activity of the Wnt signaling pathway or Wnt transcription (products). The compositions can be formulated as described herein and used for the treatment of conditions associated with Wnt transcription products or Wnt signaling pathway activity.

[00101] In one or more embodiments, the condition associated with Wnt transcription products or Wnt signaling pathway activity is a chronic wound, an acute wound, an alkali-burned corneal wound, a bum, a lesion (including lesions caused by HPV and / or a vims selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), a cartilage disease (including osteoarthritis, rheumatoid arthritis, internal derangement of the joints, and degenerative cartilage disease), a bone disease (including osteoporosis), organ fibrosis (including lung fibrosis, heart fibrosis, liver fibrosis, and kidney fibrosis), cancer (including melanoma, breast cancer, and prostate cancer), a de-nerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, tissue in need of neovascularization, osteoclast differentiation in need of inhibition, osteoblast differentiation disorders (where inhibition of osteoblast differentiation is needed), and / or bone destruction associated with breast cancer.

[00102] In one or more embodiments, the condition associated with Wnt transcription products or Wnt signaling pathway activity is a chronic wound, an acute wound, an alkali-burned corneal wound, an incisional wound (open or closed), a bum, a lesion (including lesions caused by HPV and / or a virus selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), a cartilage disease (including osteoarthritis, rheumatoid arthritis, internal derangement of the joints, and degenerative cartilage disease), a bone disease (including osteoporosis), organ fibrosis (including lung fibrosis, heart fibrosis, liver fibrosis, and kidney fibrosis), cancer (including melanoma, breast cancer, and prostate cancer), a de-nerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, tissue in need of neovascularization, osteoclast differentiation in need of inhibition, osteoblast differentiation disorders (where inhibition of osteoblast differentiation is needed), and / or bone destruction associated with breast cancer.

[00103] In one or more embodiments, the condition associated with Wnt transcription products or Wnt signaling pathway activity selected from a chronic wound, an acute wound, an alkali-burned comeal wound, an incisional wound (open or closed), a bum, a lesion (including lesions caused by HPV and / or a vims selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), cancer (including melanoma and breast cancer), a de-nerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, and tissue in need of neovasculari zati on.

[00104] In one or more embodiments, a pharmaceutical composition for inhibiting Wnt transcription or Wnt signaling pathway activity is provided, comprising: a WNT inhibitor; a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein GO and HA are covalently linked via a linker, where the GO-HA is optional; a glycol; one or more solubilizers; one or more phospholipids, where the one or more phospholipids are optional; one or more carriers, where the one or more carriers are optional; a buffer, where the buffer is optional; a preservative, where the preservative is optional; and saline or water, wherein the wt% of the WNT inhibitor, optional GO-HA (when present), glycol, one or more solubilizers, one or more optional phospholipids (when present), one or more optional carriers (when present), optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and water or saline is 100 wt%; wherein the wt% ratio of the WNT inhibitor to the GO-HA, when present, is about 0.1:1 to about 12:1; wherein the one or more phospholipid and the GOHA are not optional when the WNT inhibitor is (4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound A); and wherein the pharmaceutical composition is suitable for topical administration.

[00105] In one or more embodiments, the pharmaceutical composition comprises the WNT inhibitor in an amount from about 0.01 wt% to about 2 wt%; the optional GO-HA, when present, in an amount from about 0.01 wt% to about 2 wt%; the glycol in amount from about 2 wt% to about 10 wt%; the one or more solubilizers in an amount from about 0.5 wt% to about 10 wt%; the one or more optional phospholipids, when present, in an amount from about 0.1 wt% to about 4 wt%; the one or more optional carriers, when present, in an amount from about 2 wt% to about 10 wt%; the optional stabilizer, when present, in an amount from about 0.1 wt% to about 0.5 wt%; the optional buffer, when present, in an amount from about 0.2 wt% to about 2.0 wt%; the optional preservative, when present, in an amount from about 0.025 wt% to about 1.5 wt%; saline or water; wherein the wt% of the WNT inhibitor, optional GO-HA (when present), glycol, one or more solubilizers, one or more optional phospholipids (when present), one or more optional carriers (when present), optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and water or saline is 100 wt%. Embodiment A

[00106] Embodiment Al. Provided is a pharmaceutical composition comprising a WNT inhibitor where the pharmaceutical composition is a liposomal composition (in some embodiments a water-based liposomal composition).

[00107] Embodiment A2. In an embodiment of Embodiment Al, provided is a pharmaceutical composition comprising a WNT inhibitor which is 2-(4-(trifluoromethyl)phenyl)-l,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound A) and a graphene oxide-hyaluronic acid conjugate (GO-HA), wherein the pharmaceutical composition is a liposomal composition (in some embodiments a water-based liposomal composition), and preferably wherein the pharmaceutical composition comprises one or more phospholipids.

[00108] Embodiment A3. In an embodiment of Embodiment Al or A2, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and a glycol. In an embodiment of Embodiment Al or A2, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and butylene glycol.

[00109] Embodiment A4. In an embodiment of any one of Embodiments Al to A3, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and one or more solubilizers. In an embodiment of any one of Embodiments Al to A3, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and a polysorbate 20.

[00110] Embodiment A5. In an embodiment of any one of Embodiments Al to A4, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and one or more phospholipids. In an embodiment of any one of Embodiments Al to A4, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and phosphatidylcholine.

[00111] Embodiment A6. In an embodiment of any one of Embodiments Al to A5, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and one or more carriers. In an embodiment of any one of Embodiments Al to A5, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and ethanol.

[00112] Embodiment A7. In an embodiment of any one of Embodiments Al to A6, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and one or more optional thickeners.

[00113] Embodiment A7a. In an embodiment of any one of Embodiments Al to A7, one or more thickeners are present.

[00114] Embodiment A7b. In an embodiment of any one of Embodiments Al to A7a, the pharmaceutical composition comprises a matrix component comprising a graphene oxide (GO) and hyaluronic acid (HA) conjugate (GO-HA), wherein the GO and HA are covalently linked via a linker; XAV939; water or saline; and hydroxypropylcellulose.

[00115] Embodiment A7c. In an embodiment of any one of Embodiments Al to A7, the one or more thickeners are not present.

[00116] Embodiment A8. In an embodiment of any one of Embodiments Al to A7, the pharmaceutical composition comprises XAV939, a glycol, one or more solubilizers, one or more phospholipids, one or more carriers, GO-HA, one or more optional thickeners, and water or saline.

[00117] Embodiment A8a. In an embodiment of any one of Embodiments Al to A7b and A8, the pharmaceutical composition comprises XAV939, butylene glycol, a polysorbate 20, phosphatidylcholine, ethanol, GO-HA, and water or saline.

[00118] Embodiment A8b. In an embodiment of any one of Embodiments Al to A7b and A8, the pharmaceutical composition comprises XAV939, butylene glycol, a polysorbate 20, phosphatidylcholine, ethanol, GO-HA, water or saline, and hydroxypropylcellulose. Wnt Inhibitor

[00119] The aspects and embodiments described herein include pharmaceutical compositions comprising the recited Wnt inhibitor compounds as well as a pharmaceutically acceptable salt thereof and / or an isomer thereof. For instance, aspects and embodiments described herein include pharmaceutical compositions with a single stereoisomer of mixture of stereoisomers thereof, and / or a pharmaceutically acceptable salt thereof.

[00120] Included herein, if chemically possible, are all stereoisomers of the compounds, including diastereomers and enantiomers. Also included are mixtures of possible stereoisomers in any ratio, including, but not limited to, racemic mixtures. Unless stereochemistry is explicitly indicated in a structure at a particular atom, the structure is intended to embrace all possible stereoisomers of the compound depicted. If stereochemistry is explicitly indicated for one portion or portions of a molecule, but not for another portion or portions of a molecule, the structure is intended to embrace all possible stereoisomers for the portion or portions where stereochemistry is not explicitly indicated. It will be apparent that certain structures recite specific stereochemistry at particular atoms. Wnt Inhibitor Embodiment B

[00121] Embodiment Bl: In one or more embodiments, the pharmaceutical composition O I A II J R1 Rla comprises a WNT inhibitor according to a Wnt inhibitor of Formula (I):                 (I), or a single stereoisomer or mixture of stereoisomers thereof; a single tautomer or mixture of tautomers thereof; and / or a pharmaceutically acceptable salt thereof; wherein Formula (I) is as defined in the fourth aspect or any embodiments thereof.

[00122] Embodiment B2: In one or more embodiments, a pharmaceutical composition herein includes a Wnt inhibitor that is an inhibitor of the Wnt pathway.

[00123] Embodiment B3: In one or more embodiments of the pharmaceutical composition described herein, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is an inhibitor of the Wnt pathway, with a chemical name (4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 1). O S^Nj^NH ^^B-OH i The structure of Compound 1 is:                  0H . In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is Compound 1 or any prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or a single tautomer or mixture of tautomers thereof.

[00124] Embodiment B4: In one or more embodiments of the pharmaceutical composition described herein, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is an inhibitor of the Wnt pathway, with a chemical name 2-(4-(6-bromopyri din-3-yl) phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one (Compound 7). The structure of Compound 7 is: Rr . In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is Compound 7 or any prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or a single tautomer or mixture of tautomers thereof.

[00125] Embodiment B5: In one or more embodiments of the pharmaceutical composition herein, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is an inhibitor of the Wnt pathway, with a chemical name 2-(4-(2-(2- hydroxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 8). The structure of Compound 8 is: . In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is Compound 8 or any prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or a single tautomer or mixture of tautomers thereof.

[00126] Embodiment B6: In one or more embodiments of the pharmaceutical composition described herein, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is an inhibitor of the Wnt pathway, with a chemical name (4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid O ^iQl. (Compound 10). The structure of Compound 10 is:                        B(OH)2 jn one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is Compound 10 or any prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or a single tautomer or mixture of tautomers thereof.

[00127] Embodiment B7: In one or more embodiments of the pharmaceutical composition described herein, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is an inhibitor of the Wnt pathway, with a chemical name methyl (2-((2-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)propan-2- yl)oxy)ethyl)carbamate (Compound 18). The structure of Compound 18 is: . In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is Compound 18 or any prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or a single tautomer or mixture of tautomers thereof.

[00128] Embodiment B8: In one or more embodiments of the pharmaceutical composition described herein, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is an inhibitor of the Wnt pathway, with a chemical name 2-(4-(trifluoromethyl)phenyl)-l,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one O (Compound A). The structure of Compound A is:                     3 . In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor of Formula (I) is Compound A or any prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or a single tautomer or mixture of tautomers thereof. Wnt Inhibitor Embodiment C

[00129] Embodiment Cl: In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R1 and Rla are independently selected from is hydrogen and deuterium.

[00130] Embodiment C2: In one or more embodiments, including any one of the first, second, third, fourth, and fifth aspects and Embodiment Cl, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein ring A is or o. D D , and wherein < designates attachment to the remainder of the Wnt inhibitor of Formula (I). Wnt Inhibitor Embodiments where R2 is (a)

[00131] Embodiment C3: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is phenyl substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups.

[00132] Embodiment C4: In one or more embodiments, including of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C3, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is attached to the para-position of the phenyl ring; or R3 and one R3a, when on adjacent carbons, together with the carbons to which they are attached form ring (a-1) and where the phenyl portion is optionally substituted with the remaining R3a groups.

[00133] Embodiment C5: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C4, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein when R2 is (a), R3 is not halo or haloalkyl.

[00134] Embodiment C6: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C4, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein when R2 is (a), R3 is B(O)2 or -(Co-Cealkylene)-O-R4.

[00135] Embodiment C7: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C6, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is R3a R3a R3 R3a . In one or more embodiments, R3 is not halo or haloalkyl. In one or more embodiments, R3 is B(O)2 or -(Co-C6alkylene)-0-R4.

[00136] Embodiment C8: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C4, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is

[00137] Embodiment C9: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C4, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is selected from the group consisting of Wnt Inhibitor Embodiments where R2 is (b)

[00138] Embodiment CIO: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is phenyl substituted with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups and where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups.

[00139] Embodiment Cll: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2 and CIO, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is phenyl substituted at its para-position with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups and where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups; and when the 5- or 6-membered heteroaryl is a 6-membered heteroaryl then R3 is substituted on the para-position of the 6-membered heteroaryl.

[00140] Embodiment C12: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, CIO, and Cll, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I),

[00141] Embodiment C13: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and CIO to C12, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), Wnt Inhibitor Embodiments where R2 is (c)

[00142] Embodiment C14: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is phenyl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl-R3 where the phenyl in -phenyl-R3 is optionally substituted with 1, 2, or 3 R3a groups.

[00143] Embodiment C15: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and Cl 4, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is phenyl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted at its para-position with -phenyl-R3 where the phenyl in -phenyl-R3 is optionally substituted with 1, 2, or 3 R3a groups and where the R3 is in the para-position of the phenyl in -phenyl-R3.

[00144] Embodiment C16: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, Cl 4, and C15, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), 3 wherein R2 is selected from the group consisting of                    R , R3a Ai A. A" \^R3          \^R3    and R3a Ax

[00145] Embodiment C17: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, Cl 4, and C15, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), aQa. wherein R2 is selected from the group consisting of                    B(OH)2, Wnt Inhibitor Embodiments where R2 is (d)

[00146] Embodiment C18: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is 5- to 6- membered heteroaryl substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups; optionally wherein the R3 is at the para-position of the 6-membered heteroaryl.

[00147] Embodiment C19: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and Cl 8, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein when R2 is (d), R3 is not halo or haloalkyl.

[00148] Embodiment C20: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and Cl 8, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein when R2 is (d), R3 is B(O)2 or -(Co-C6alkylene)-0-R4. Wnt Inhibitor Embodiments where R2 is (e)

[00149] Embodiment C22: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is 5-or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl-R3 where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups; optionally wherein the -phenyl-R3 is at the para-position of the 6-membered heteroaryl; and optionally wherein the R3 is at the para-position of the phenyl. Wnt Inhibitor Embodiments where R2 is (f)

[00150] Embodiment C23: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is 5-or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -(5- or 6-membered heteroaryl)-R3 where the 5- or 6-membered heteroaryl in -(5- or 6-membered heteroaryl)-R3 is optionally substituted with 1, 2, or 3 R3a groups; optionally wherein the -(6-membered heteroaryl)-R3 is at the para-position of the first 6-membered heteroaryl; and optionally wherein the R3 is at the para-position of the 6membered heteroaryl to which it is attached.

[00151] Embodiment C24: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and C23, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein Wnt Inhibitor Embodiments where R2 is (g)

[00152] Embodiment C25: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is Cs-Cecycloalkyl substituted with R3 and additionally optionally substituted with 1 or 2 R3a groups.

[00153] Embodiment C26: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and C25, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is           R3, optionally wherein R3 is 5- to 10-membered heterocyclic optionally substituted with cyano. Wnt Inhibitor Embodiments where R2 is (h)

[00154] Embodiment C27: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is Cs-Cecycloalkyl substituted with 5- or 6-membered heteroaryl where the 5- or 6membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups, and where the cycloalkyl is optionally substituted with 1 or 2 R3a groups. Wnt Inhibitor Embodiments where R2 is (i)

[00155] Embodiment C28: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is Cs-Cecycloalkyl substituted with phenyl where the phenyl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups, and where the cycloalkyl is optionally substituted with 1 or 2 R3a groups. Wnt Inhibitor Embodiments where R2 is (j)

[00156] Embodiment C29: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is 3- to 8-membered heterocycloalkyl substituted with phenyl or 5- or 6- membered heteroaryl, where the phenyl and the 5- to 6- membered heteroaryl are substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups.

[00157] Embodiment C30: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and C29, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein Wnt Inhibitor Embodiments where R2 is (k)

[00158] Embodiment C31: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl and C2, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is -CH=CH-R5 where R5 is phenyl or 5- or 6- membered heteroaryl, where the phenyl and the 5- or 6- membered heteroaryl are substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups.

[00159] Embodiment C32: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, and C31, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R2 is selected from the group consisting of               R3 and               R . Wnt Inhibitor Additional Embodiments

[00160] Embodiment C33: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C5, C7, CIO to C12, C14 to C16, C18 to C19, and C22 to C32 and any embodiments provided above when R2 is (a)-(k), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is cyano, -B(OH)2, or -(Co-C6alkylene)-0-R4. In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is hydroxy-Ci-Cealkyl (in one or more embodiments, hydroxy ethyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxy-Ci-Cealkyl (in one or more embodiments, Ci-Cealkoxy-C2alkyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl (in one or more embodiments, Ci-C6alkoxycarbonyl-NH-C2alkyl).

[00161] Embodiment C34: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C5, C7, CIO to C12, C14 to C16, C18 to C19, and C22 to C32 and any embodiments provided above when R2 is (a)-(k), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is cyano.

[00162] Embodiment C35: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C5, C7, CIO to C12, C14 to C16, C18 to C19, and C22 to C32 and any embodiments provided above when R2 is (a)-(k), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is -B(OH)2.

[00163] Embodiment C36: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C5, C7, CIO to C12, C14 to C16, C18 to C19, and C22 to C32 and any embodiments provided above when R2 is (a)-(k), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is -(Co-C6alkylene)-0-R4. In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is hydroxy-Ci-Cealkyl (in one or more embodiments, hydroxy ethyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxy-Ci-Cealkyl (in one or more embodiments, Ci-Cealkoxy-C2alkyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl (in one or more embodiments, Ci-C6alkoxycarbonyl-NH-C2alkyl).

[00164] Embodiment C37: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C5, C7, CIO to C12, C14 to C16, C18 to C19, and C22 to C32 and any embodiments provided above when R2 is (a)-(k), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is -(Ci-C6alkylene)-O-R4. In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is hydroxy-Ci-Cealkyl (in one or more embodiments, hydroxy ethyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxy-Ci-Cealkyl (in one or more embodiments, Ci-Cealkoxy-C2alkyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl (in one or more embodiments, Ci-C6alkoxycarbonyl-NH-C2alkyl).

[00165] Embodiment C38: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, CIO to Cl2, C14 to C16, and C22 to C24 and any embodiments provided above when R2 is (b), (c), (e), or (f) provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is halo, cyano, -B(OH)2, or -(Co-C6alkylene)-0-R4. In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is hydroxy-Ci-Cealkyl (in one or more embodiments, hydroxy ethyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxy-Ci-Cealkyl (in one or more embodiments, Ci-Cealkoxy-C2alkyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl (in one or more embodiments, Ci-C6alkoxycarbonyl-NH-C2alkyl).

[00166] Embodiment C39: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl, C2, CIO to C12, C14 to C16, and C22 to C24 and any embodiments provided above, when R2 is (b), (c), (e), or (f), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is halo, cyano, -B(OH)2, or -(Co-C6alkylene)-0-R4; or when R2 is (b), (c), (e), or (f), provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R3 is cyano, -B(OH)2, or -(Co-Cealkylene)-O-R4.

[00167] Embodiment C40: In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects and Embodiments Cl to C5, C7, CIO to C12, C14 to C16, C18 to C19, and C22 to C32 wherein R4 is hydroxy-Ci-Cealkyl (in one or more embodiments, hydroxy ethyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxy-Ci-Cealkyl (in one or more embodiments, Ci-Cealkoxy-C2alkyl). In one or more embodiments, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), wherein R4 is Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl (in one or more embodiments, Ci-Cealkoxycarbonyl-NH-C2alkyl).

[00168] Provided herein are any one of claims 1-3 and 5-49 wherein the WNT inhibitor is according to any one of Embodiments Cl to C40.

[00169] Embodiment C41: In one or more embodiments, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects, provided is a pharmaceutical composition comprising a Wnt inhibitor of Formula (I), selected from the group consisting of the compounds provided in Table 1.

[00170] Embodiment C42: In one or more embodiments of the pharmaceutical composition, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor is according to a Wnt inhibitor of Formula (I), the compound is selected from any of Compounds 1-44 or a pharmaceutically acceptable salt thereof, from Table 1. In one or more embodiments of the pharmaceutical composition, the Wnt inhibitor is according to a Wnt inhibitor of Formula (I), the compound is selected from any of Compounds 1-44, or an isomer thereof, from Table 1. In one or more embodiments of the pharmaceutical composition, the Wnt inhibitor is according to a Wnt inhibitor of Formula (I), the compound is selected from any of Compounds 1-44, or a single stereoisomer of mixture of stereoisomers thereof, and / or a pharmaceutically acceptable salt thereof, from Table 1. In one or more embodiments of the pharmaceutical composition, the Wnt inhibitor is according to a Wnt inhibitor of Formula (I), the compound is selected from any of Compounds 1-44, or a prodrug, pharmaceutically acceptable salt, metabolite, polymorph, solvate, hydrate, stereoisomer, or tautomer thereof, from Table 1. Table 1. Wnt inhibitor compounds Compound Number Structure A (XAV939) Wnt Inhibitor - Amounts

[00171] In one or more embodiments of the pharmaceutical composition, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor, such as a Wnt inhibitor of Formula (I) (for example, Compound 1, Compound 7, Compound 8, Compound 10, Compound 18) can constitute from about 0.001 wt% to about 5 wt% of the total composition (including water). For example, the WNT inhibitor can be about 0.005 wt% to about 5 wt%, from about 0.005 wt% to about 4.5 wt%, from about 0.005 wt% to about 4 wt%, from about 0.005 wt% to about 3.5 wt%, from about 0.005 wt% to about 3 wt%, from about 0.005 wt% to about 2.5 wt%, from about 0.005 wt% to about 2 wt%, from about 0.005 wt% to about 1.5 wt%, from about 0.005 wt% to about 1 wt%, from about 0.005 wt% to about 0.1 wt% of the total composition wt%.

[00172] In one or more embodiments of the pharmaceutical composition, including the Wnt Inhibitor of any one of the first, second, third, fourth, and fifth aspects, the Wnt inhibitor, such as a Wnt inhibitor of Formula (I) (for example, Compound 1, Compound 7, Compound 8, Compound 10, Compound 18) can constitute from about 0.01 wt% to about 5 wt% of the total composition (including water). For example, the WNT inhibitor can be from about 0.01 wt% to about 5 wt%, 0.01 wt% to about 4.5 wt%, from about 0.01 wt% to about 4 wt%, from about 0.01 wt% to about 3.5 wt%, from about 0.01 wt% to about 3 wt%, from about 0.01 wt% to about 2.5 wt%, from about 0.01 wt% to about 2 wt%, from about 0.01 wt% to about 1.5 wt%, from about 0.01 wt% to about 1 wt%, or from about 0.01wt% to about 0.1 wt%, from about 0.02 wt% to about 1 wt%, or from about 0.05 wt% to about 0.5 wt% of the total composition wt%.

[00173] The concentration of WNT inhibitor in the pharmaceutical composition can be from about 0.05 to about 20 mg / mL, from 0.05 to 20 mg / mL, from about 0.05 to about 15 mg / mL, from 0.05 to 15 mg / mL, from about 0.05 to about 10 mg / mL, from 0.05 to 10 mg / mL, from about 0.1 to about 20 mg / mL, from 0.1 to 20 mg / mL, from about 0.1 to about 15 mg / mL, from 0.1 to 15 mg / mL, from about 0.1 to about 10 mg / mL, from 0.1 to 10 mg / mL, from about 0.1 to about 5 mg / mL, or from 0.1 to 5 mg / mL of the total composition wt%.

[00174] In one or more embodiments, the concentration of WNT inhibitor in the pharmaceutical composition is from about 0.1 to about 10 mg / mL, or from 0.1 to 10 mg / mL.

[00175] In one or more embodiments, the concentration of XAV939 when present in the pharmaceutical composition is from about 0.1 mg / mL to about 10 mg / mL, or from 0.1 mg / mL to 10 mg / mL of the total composition wt%. Wnt Inhibitor - Optically Active Compounds

[00176] It is appreciated that compounds provided herein have several chiral centers and may exist in and be isolated in optically active and racemic forms. It is to be understood that any racemic, optically-active, diastereomeric, tautomeric, or stereoisomeric form, mixture, or combination thereof, of a compound provided herein, which possess the useful properties described herein is within the scope of the present disclosure. It being well known in the art how to prepare optically active forms (in some or any embodiments, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase).

[00177] In some or any embodiments, methods to obtain optically active materials are known in the art and include at least the following. i) physical separation of crystals - a technique whereby macroscopic crystals of the individual stereoisomers are manually separated. This technique can be used if crystals of the separate stereoisomers exist, i.e., the material is a conglomerate, and the crystals are visually distinct; ii) simultaneous crystallization - a technique whereby the individual stereoisomers are separately crystallized from a solution of the racemate, possible if the latter is a conglomerate in the solid state; iii) enzymatic resolutions - a technique whereby partial or complete separation of a racemate by virtue of differing rates of reaction for the stereoisomers with an enzyme; iv) enzymatic asymmetric synthesis - a synthetic technique whereby one or more step(s) of the synthesis uses an enzymatic reaction to obtain a stereoisomerically pure or enriched synthetic precursor of the desired stereoisomer; v) chemical asymmetric synthesis - a synthetic technique whereby the desired stereoisomer is synthesized from an achiral precursor under conditions that produce asymmetry (i.e., chirality) in the product, which may be achieved using chiral catalysts or chiral auxiliaries; vi) diastereomer separations - a technique whereby a racemic compound is reacted with an enantiomerically pure reagent (the chiral auxiliary) that converts the individual enantiomers to diastereomers. The resulting diastereomers are then separated by chromatography or crystallization by virtue of their now more distinct structural differences and the chiral auxiliary later removed to obtain the desired enantiomer; vii) first- and second-order asymmetric transformations - a technique whereby diastereomers from the racemate equilibrate to yield a preponderance in solution of the diastereomer from the desired enantiomer or where preferential crystallization of the diastereomer from the desired enantiomer perturbs the equilibrium such that eventually in principle all the material is converted to the crystalline diastereomer from the desired enantiomer. The desired enantiomer is then released from the diastereomer; viii) kinetic resolutions - this technique refers to the achievement of partial or complete resolution of a racemate (or of a further resolution of a partially resolved compound) by virtue of unequal reaction rates of the stereoisomers with a chiral, non-racemic reagent or catalyst under kinetic conditions; ix) stereospecific synthesis from non-racemic precursors - a synthetic technique whereby the desired stereoisomer is obtained from non-chiral starting materials and where the stereochemical integrity is not or is minimally compromised over the course of the synthesis; x) chiral liquid chromatography - a technique whereby the stereoisomers of a racemate are separated in a liquid mobile phase by virtue of their differing interactions with a stationary phase. The stationary phase can be made of chiral material or the mobile phase can contain an additional chiral material to provoke the differing interactions; xi) chiral gas chromatography - a technique whereby the racemate is volatilized and stereoisomers are separated by virtue of their differing interactions in the gaseous mobile phase with a column containing a fixed non-racemic chiral adsorbent phase; xii) extraction with chiral solvents - a technique whereby the stereoisomers are separated by virtue of preferential dissolution of one stereoisomer into a particular chiral solvent; xiii) transport across chiral membranes - a technique whereby a racemate is placed in contact with a thin membrane barrier. The barrier typically separates two miscible fluids, one containing the racemate, and a driving force such as concentration or pressure differential causes preferential transport across the membrane barrier. Separation occurs as a result of the non-racemic chiral nature of the membrane which allows one stereoisomer of the racemate to pass through.

[00178] In some or any embodiments, provided is a pharmaceutical composition with a compound that comprises a substantially pure designated stereoisomer of the compound. In some or any embodiments, in the methods and compounds, the compounds are substantially free of other stereoisomer. In some or any embodiments, a pharmaceutical composition includes a compound that is at least 85%, 90%, 95%, 98%, 99% or 100% by weight, of the designated stereoisomer, the remainder comprising other chemical species or stereoisomers. Wnt Inhibitor - Isotopically Enriched Compounds

[00179] Also provided herein are isotopically enriched compounds.

[00180] Isotopic enrichment (in some or any embodiments, deuteration) of pharmaceuticals to improve pharmacokinetics (“PK”), pharmacodynamics (“PD”), and toxicity profiles, has been demonstrated previously with some classes of drugs. See, for example, Lijinsky et. al., Food Cosmet. Toxicol., 20: 393 (1982); Lijinsky et. al., J. Nat. Cancer Inst., 69: 1127 (1982); Mangold et. al., Mutation Res. 308: 33 (1994); Gordon et. al., Drug Metab. Dispos., 15: 589 (1987); Zello et. al., Metabolism, 43: 487 (1994); Gately et. al., J. Nucl. Med., 27: 388 (1986); WadeD, Chern. Biol. Interact. 117: 191 (1999).

[00181] Isotopic enrichment of a drug can be used, in some or any embodiments, to (1) reduce or eliminate unwanted metabolites, (2) increase the half-life of the parent drug, (3) decrease the number of doses to achieve a desired effect, (4) decrease the amount of a dose that achieves a desired effect, (5) increase the formation of active metabolites, if any are formed, and / or (6) decrees the production of deleterious metabolites in specific tissues and / or create a more effective drug and / or a safer drug for combination therapy, whether the combination therapy is intentional or not.

[00182] Replacement of an atom for one of its isotopes often will result in a change in the reaction rate of a chemical reaction. This phenomenon is known as the Kinetic Isotope Effect (“KIE”). For example, if a C-H bond is broken during a rate-determining step in a chemical reaction (i.e. the step with the highest transition state energy), substitution of a deuterium for that hydrogen will cause a decrease in the reaction rate and the process will slow down. This phenomenon is known as the Deuterium Kinetic Isotope Effect (“DKIE”). See, e.g., Foster et aL, Adv. Drug Res., vol. 14, pp. 1-36 (1985); Kushner et al.. Can. J. Physiol. Pharmacol., vol. 77, pp. 79-88 (1999). The highest transition state energy may refer to the largest Gibbs free energy or largest Gibbs free energy difference relative to starting material or intermediate of interest in a reaction pathway depicted by a single energy axis.

[00183] The magnitude of the DKIE can be expressed as the ratio between the rates of a given reaction in which a C-H bond is broken, and the same reaction where deuterium is substituted for hydrogen. The DKIE can range from about 1 (no isotope effect) to large integers, such as 50 or more, meaning that the reaction can be fifty, or more, times slower when deuterium is substituted for hydrogen. High DKIE values may be due in part to a phenomenon known as tunneling, which is a consequence of the uncertainty principle. Tunneling is ascribed to the small mass of a hydrogen atom and occurs because transition states involving a proton can sometimes form in the absence of the requisite activation energy. Because deuterium has more mass than hydrogen, it statistically has a much lower probability of undergoing this phenomenon.

[00184] Tritium (“T”) is a radioactive isotope of hydrogen, used in research, fusion reactors, neutron generators and radiopharmaceuticals. Tritium is a hydrogen atom that has 2 neutrons in the nucleus and has an atomic weight close to 3. It occurs naturally in the environment in low concentrations, commonly found as T2O. Tritium decays slowly (half-life = 12.3 years) and emits a low energy beta particle that cannot penetrate the outer layer of human skin. Internal exposure is the main hazard associated with this isotope, yet ingesting in large amounts can pose a significant health risk. As compared with deuterium, a lesser amount of tritium must be consumed before it reaches a hazardous level. Substitution of tritium (“T”) for hydrogen results in yet a stronger bond than deuterium and gives numerically larger isotope effects. Similarly, substitution of isotopes for other elements, including, but not limited to, 13C or 14C for carbon,33S, 34S, or 36S for sulfur, 15N for nitrogen, and 17O or 18O for oxygen, may lead to a similar kinetic isotope effect.

[00185] For example, the DKIE was used to decrease the hepatotoxicity of halothane by presumably limiting the production of reactive species such as trifluoroacetyl chloride. However, this method may not be applicable to all drug classes. For example, deuterium incorporation can lead to metabolic switching. The concept of metabolic switching asserts that xenogens, when sequestered by Phase I enzymes, may bind transiently and re-bind in a variety of conformations prior to the chemical reaction (e.g., oxidation). This hypothesis is supported by the relatively vast size of binding pockets in many Phase I enzymes and the promiscuous nature of many metabolic reactions. Metabolic switching can potentially lead to different proportions of known metabolites as well as altogether new metabolites. This new metabolic profile may impart more or less toxicity.

[00186] In one or more embodiments, the compounds described herein may be used as radiopharmaceuticals such as, for example, imaging agents. In one instance, radiopharmaceuticals are positron emission tomography (PET) imaging agents. In such embodiments, substitution of radionuclides (e.g., positron emitting isotopes) for atoms in the compounds allows for the syntheses of radiopharmaceuticals that can function as imaging agents. In one or more embodiments, radionuclides which can be substituted in the compounds described herein include, and are not limited to, 18F, nC, 13N, 15O, 76Br, and 124I. In one or more embodiments, the compound is isotopically enriched at one or more atoms, one atom, two atoms, or three atoms. In one or more embodiments, the compound is administered as an isotopic composition.

[00187] The animal body expresses a variety of enzymes for the purpose of eliminating foreign substances, such as therapeutic agents, from its circulation system. In some or any embodiments, such enzymes include the cytochrome P450 enzymes (“CYPs”), esterases, proteases, reductases, dehydrogenases, and monoamine oxidases, to react with and convert these foreign substances to more polar intermediates or metabolites for renal excretion. Some common metabolic reactions of pharmaceutical compounds involve the oxidation of a carbonhydrogen (C-H) bond to either a carbon-oxygen (C-O) or carbon-carbon (C-C) pi-bond. The resultant metabolites may be stable or unstable under physiological conditions, and can have substantially different pharmacokinetic, pharmacodynamic, and acute and long-term toxicity profiles relative to the parent compounds. For many drugs, such oxidations are rapid. These drugs therefore often require the administration of multiple or high daily doses.

[00188] Therefore, isotopic enrichment at certain positions of a compound provided herein will produce a detectable KIE that will affect the pharmacokinetic, pharmacologic, and / or toxicological profiles of a compound provided herein in comparison with a similar compound having a natural isotopic composition. Preparation of Wnt Inhibitor Compounds

[00189] The Wnt inhibitors provided herein can be prepared, isolated, or obtained by any method apparent to those of skill in the art. Wnt inhibitors provided herein can be prepared according to the Preparation Schemes provided below. Reaction conditions, steps and reactants not provided in the Preparation Schemes described herein would be apparent to, and known by, those skilled in the art.

[00190] Additional steps and reagents not provided in the Preparation Schemes described herein would be known to those of skill in the art. For example, intermediates and compounds could be prepared using the procedures known by one of ordinary skill in the art or as disclosed in U.S. Provisional Application Numbers: 63 / 417,257; 63 / 418,947; and 63 / 418,956 (wherein the synthetic methods disclosed therein are herein incorporated by reference in their entirety). Methods of preparation are described in detail in the Examples herein.

[00191] In one or more embodiments, provided is a method of preparing a Wnt inhibitor of R2 -H, wherein LG1 is fluoro, chloro, bromo, iodo, tritiate, mesylate, a triazole, a pyrazole, boronic acid, boronic ester, or aryl trifluoroborate; and optionally isolating the Wnt inhibitor of Formula (I); wherein R2' is (bl) phenyl substituted with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with LG1 and additionally optionally substituted with 1, 2, or 3 R3a groups and where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups, (cl) phenyl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl- LG1 where the phenyl in -phenyl- LG1 is optionally substituted with 1, 2, or 3 R3a groups; (el) 5- or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl- LG1 where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups; (fl) 5- or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -(5- or 6-membered heteroaryl)- LG1 where the 5- or 6-membered heteroaryl in -(5- or 6-membered heteroaryl)-LG1 is optionally substituted with 1, 2, or 3 R3a groups; (hl) Cs-Cecycloalkyl substituted with NH2 or OH and additionally optionally substituted with 1 or 2 R3a groups; or (il) Cs-Cecycloalkyl substituted with phenyl where the phenyl is substituted with LG1 and the phenyl is additionally optionally substituted with 1,2, or 3 R3a groups, and where the cycloalkyl is optionally substituted with 1 or 2 R3a groups; and provided that the compound is not: methyl O s^^^oMe 4-oxotetrahydro-2H-thiopyran-3-carboxylate 0     ; or a salt thereof and / or a stereoisomer or mixture of stereoisomers thereof. In one or more embodiments, methods of preparing a Wnt inhibitor of Formula (I) include contacting under basic conditions; and all other groups are as defined herein in any embodiments; and optionally wherein R1 and Rla are independently hydrogen or alkyl.

[00192] One of skill will understand that the order of steps for any process described herein may be changed. Other variations will be apparent to one of skill in the art and all such variations are contemplated within the scope of embodiments presented herein. Active pharmaceutical agent

[00193] While the WNT inhibitors disclosed herein can be administered as the sole active pharmaceutical agent, the sole active pharmaceutical agent used to treat a particular disorder, or the sole active pharmaceutical agent in a therapeutically effective amount in a composition, they can also be used in combination with one or more other agents used in the treatment or suppression of certain disorders.

[00194] In one or more embodiments, the pharmaceutical compositions provided herein do not comprise an FGF agonist, a BMP inhibitor, a TGF-P agonist, and / or a DMP4 agonist.

[00195] In some or any embodiments, the WNT inhibitor is not a protein or peptide. GO-HA

[00196] Embodiment DI: The covalently-linked GO and HA is also referred to herein as GO-HA conjugate or simply GO-HA. The GO-HA conjugate can be made according to procedures known to a person of ordinary skill in the art, including those disclosed in US-2019-01053 98-Al.

[00197] Graphene oxide (GO) as used herein refers to an oxidized form of graphene, which is a single layer form of graphite. GO can be obtained by treating graphite with strong oxidizers. GO contains carbon, oxygen, and hydrogen in various amounts, depending on how it is made. It can be of length of several hundreds of nanometers up to several micrometers, its planar direction, and about 0.7-1.2 nm in thickness. GO can include various oxygen containing moieties, such as oxygen epoxide groups, carboxylic acid (-COOH), phenol, etc., when prepared using sulfuric acid (e.g. Hummer’s method). An example GO structure is shown below.

[00198] In one or more embodiments, graphene oxide is modified by reacting with chloroacetic acid, herein referred to as “Modified GO” in some embodiments. In one or more embodiments, hydroxyl groups of the graphene oxide react with the chloroacetic acid, i.e. replacing -OH groups with -OCH2C(O)OH groups.

[00199] Hyaluronic acid (HA) is an anionic, highly hydrophilic, non-sulfated glycosaminoglycan, occurring naturally throughout the human body. It can be several thousands of carbohydrate units long and can bind to water giving it a gel of stiff viscous quality. An example structure of HA is provided below:

[00200] In a composition of present disclosure, the GO or Modified GO and HA are covalently linked to form a matrix component (or a carrier), which can serve to form a stable suspension of the Wnt inhibitor, including of Formula (I) (such as XAV939, Compound 1, Compound 7, Compound 8, Compound 10, and Compound 18) as well as providing other simultaneous benefits to wound healing.

[00201] Embodiment D2: The covalent linking can be accomplished by using a linker or linker moiety (“GO-HA linker”). In one or more embodiments, the GO-HA linker can include 2-25 carbons. In one or more embodiments, the GO-HA linker is linear. In one or more embodiments, the GO-HA linker is branched. The GO-HA linker can be saturated or unsaturated.

[00202] Embodiment D3: In one or more embodiments, the GO-HA linker can comprise a C2-C25 alkylene group, where the carbons and hydrogens in the alkylene group can be substituted by oxygen or other atoms or groups such as hydroxy, carboxy, amino, alkyl, alkoxy, alkenyl, alkynyl, nitro, etc. In one or more embodiments, the GO-HA linker can comprise one or more -CH2CH2O- units. In one or more embodiments, the GO-HA linker does not comprise dopamine. In one or more embodiments, the GO-HA linker does not comprise an aryl or heteroaryl group.

[00203] Embodiment D4: In one or more embodiments, the GO-HA linker comprises -Rx-Rs-Ry-, wherein Rx and Ry are each independently selected from the group consisting of -CO-, -COO-, - H-, - H- H-, - H- H- CO-, -CS-, -S-, -O-, and wherein Rs (which is also referred to as the spacer group in this application) can be an unsubstituted or substituted, saturated or unsaturated linear alkylene group having 2-20 backbone carbons. In one or more embodiments, both Rx and Ry are *- H- H-CO- (* denoting the ends of the linker distal to Rs).

[00204] Embodiment D5: In one or more embodiments, the spacer group in the GO-HA linker can be an unsubstituted or substituted, saturated or unsaturated linear alkylene group having 2-20 backbone carbons. For illustration and not limitation, the HA can be derivatized with one of the following spacer groups: 1 of                  R102 where R101 and R102 can be independently -CONHNH-, -S-, -NH-, -0-, or other nucleophiles, and n is an integer and can be for example, 1-20, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. In one or more embodiments, the HA is derivatized with a spacer group comprising a dihydrazide (e.g. -NHNHC(O)-alkylene-CONHNH-), such as adipic acid dihydrazide (-NHNHC(O)(CH2)4CONHNH-). The derivatized HA can then be linked to GO or Modified GO.

[00205] Embodiment D6a: In some or any embodiments, the GO-HA conjugate is a conjugate of GO linked to HA via a linker comprising -CH2-C(O)-NH-NH-C(O)-(CH2)4-C(O)NH-NH-.

[00206] Embodiment D6: In some or any embodiments, the GO-HA conjugate is a conjugate of GO linked to HA via the linker -CH2-C(O)-NH-NH-C(O)-(CH2)4-C(O)NH-NH-where the HA is linked via an amide bond comprising the terminal NH of the linker and the GO is linked to the CH2 end of the linker (in some embodiments through an ether bond with GO phenol). Wnt Inhibitor to GO-HA weight ratio (wt% ratio)

[00207] In one or more embodiments of the pharmaceutical composition comprising GOHA (for example, GO-HA of Embodiments D6 and D6a), the wt% ratio of the Wnt inhibitor, such as a Wnt inhibitor of Formula (I) (for example, XAV939, Compound 1, Compound 7, Compound 8, Compound 10, and Compound 18) to GO-HA can be from about 1:100 to 100:1, e.g., from about 1:2 to about 2:1 in the total composition. For example, the wt% ratio of the Wnt inhibitor to GO-HA can be 1:100, about 1:90, about 1:80, about 1:70, about 1:60, about 1:50, about 1:40, about 1:30, about 1:20, about 1:10, about 1:5, about 1:1, about 5:1, about 10:1, about 20:1, about 30:1, about 40:1, about 50:1, about 60:1, about 70:1, about 80:1, about 90:1, or 100:1 in the total composition. In one or more embodiments, the wt% ratio of the Wnt inhibitor to GO-HA can be from about 0.1:1 to about 12:1, such as from 0.1:1, about 0.2:1, about0.3:l, about0.4:l, about0.5:l, about 1:1, about2:l, about3:l, about4:l, about5:l, about 6:1, about 7:1, about 8:1, about 9:1, about 10:1, about 11:1, or 12:1 in the total composition.

[00208] In one or more embodiments, the wt% ratio of WNT inhibitor:GO-HA in the pharmaceutical composition is from about 1:0.1 to about 1:1. For example, the wt% ratio of WNT inhibitor:GO-HA in the pharmaceutical composition is from about 1:0.2 to about 1:1, from about 1:0.3 to about 1:1, from about 1:0.4 to about 1:1, from about 1:0.5 to about 1:1, from about 0.6 to about 1:1, from about 0.7 to about 1:1, from about 0.8 to about 1:1, or from about 0.9 to about 1:1. In some or any embodiments, the WNT inhibitor is XAV939, Compound 1, Compound 7, Compound 8, Compound 10, or Compound 18. In some or any embodiments, the WNT inhibitor is XAV939. GO-HA Amounts

[00209] In one or more embodiments, in the GO-HA conjugate (for example, GO-HA of Embodiments D6 and D6a), the weight ratio of GO:HA can be from about 1:1 to about 1:20, from about 1:6 to about 1:10. For example, the weight ratio of GO:HA in the GO-HA conjugate can be about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:11, about 1:12, about 1:13, about 1:14, about 1:15, about 1:16, about 1:17, about 1:18, about 1:19, or about 1:20.

[00210] In one or more embodiments, GO-HA (for example, GO-HA of Embodiments D6 and D6a) constitutes from about 0.001 wt % to about 5 wt% of the total composition. For example, the GO-HA can constitute from about 0.005 wt% to about 5 wt%, from about 0.005 wt% to about 4 wt%, from about 0.005 wt% to about 3 wt%, from about 0.005 wt% to about 2 wt%, from about 0.005 wt% to about 1 wt%, from about 0.005 wt% to about 0.5 wt%, from about 0.01 wt% to about 5 wt%, from about 0.01 wt% to about 4 wt%, from about 0.01 wt% to about 3 wt%, from about 0.01 wt% to about 2 wt%, from about 0.01 wt% to about 1 wt%, from about 0.01 wt% to about 0.5 wt%, from about 0.02 wt% to about 5 wt%, from about 0.02 wt% to about 4 wt%, from about 0.02 wt% to about 3 wt%, from about 0.02 wt% to about 2 wt%, from about 0.02 wt% to about 1 wt%, from about 0.02 wt% to about 0.5 wt%, from about 0.05 wt% to about 5 wt%, from about 0.05 wt% to about 4 wt%, from about 0.05 wt% to about 3 wt%, from about 0.05 wt% to about 2 wt%, from about 0.05 wt% to about 1 wt%, or from about 0.05 wt% to about 0.5 wt% of the total composition wt%.

[00211] The concentration of GO-HA in the pharmaceutical composition can be from 0.005 to 25 mg / mL, about 0.005 to about 25 mg / mL, from about 0.005 to about 20 mg / mL, from 0.005 to 20 mg / mL, from about 0.005 to about 15 mg / mL, from 0.005 to 15 mg / mL, from about 0.005 to about 10 mg / mL, from 0.005 to 10 mg / mL, from about 0.005 to about 5 mg / mL, from 0.005 to 5 mg / mL, from about 0.01 to about 25 mg / mL, from 0.01 to 25 mg / mL, from about 0.01 to about 20 mg / mL, from 0.01 to 20 mg / mL, from about 0.01 to about 15 mg / mL, from 0.01 to 15 mg / mL, from about 0.01 to about 10 mg / mL, or from 0.01 to 10 mg / mL of the total composition wt%.

[00212] In one or more embodiments, the concentration of GO-HA in the pharmaceutical composition is from about 0.01 to about 10 mg / mL, or from 0.01 to 10 mg / mL of the total composition wt%. Additional Components of the Composition

[00213] In the pharmaceutical compositions as described herein, other pharmaceutical or therapeutic compounds may be included in addition to a Wnt inhibitor, such as a Wnt inhibitor of Formula (I) (for example, XAV939, Compound 1, Compound 7, Compound 8, Compound 10, Compound 18). In other words, the pharmaceutical compositions, with Wnt inhibitors such as compound(s) of Formula (I) (for example, XAV939, Compound 1, Compound 7, Compound 8, Compound 10, Compound 18) present can also serve as a base dispersion medium in which other pharmaceutical or therapeutic agents, especially those which are hydrophobic, may be dispersed, e.g., for topical administration to a wound. These agents may include antifibrotic compounds such as pirfenidone, halofuginone, nintedanib, tocilizumab, rilonacept, etc., anti-cancer agents, anti-inflammatory agents, analgesics, antibiotics, Wnt inhibitors, Hedgehog pathway inhibitors, TGF-B inhibitors, LOX inhibitors, etc.

[00214] In one or more embodiments, the pharmaceutical composition further comprises pharmaceutical carriers, excipients, compounds, or materials which enables the composition to be presented in topically administrable semi-solid aqueous gel forms. For example, carboxymethylcellulose can be used as a gel-forming agent. However, other cellulose derivatives such as microcrystalline cellulose as well as polysaccharides such as alginate, agarose, tragacanth, guar gum, and xanthum gum; are also suitable as gel-forming agents. The gel may be made thicker and / or stiffer by addition of a relatively resilient gel-forming material such as a cross-linked fibrous protein, e.g. gelatin or collagen cross-linked with formaldehyde. In one or more embodiments, the pharmaceutical composition can be in a form of a cream, which can include those excipients suitable for a cream formulation, such as paraffin oil, vaseline, wax, organic esters such as cetyl palmitate, etc.

[00215] In one or more embodiments, the compositions can include a second medication or therapeutic agent to the wound, comprising one or more of corticosteroid, a cytotoxic drug, an antibiotic, an antiseptic, nicotine, an anti-platelet drug, an NSAID, colchicine, an anticoagulant, a vasoconstricting drug or an immunosuppressive, a growth factor, an antibody, a protease, a protease inhibitor, an antibacterial peptide, an adhesive peptide, a hemostatic agent, living cells, honey, or nitric oxide. These therapeutic agents can be delivered as separate dosage forms from the compositions described herein or may be included as additional components of the pharmaceutical compositions described herein, hence delivered together with the Wnt inhibitor of Formula (I) (or any embodiments thereof).

[00216] In some or any embodiments, the content of the pharmaceutical composition is described as being wherein: the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more phospholipids, one or more carriers, optional stabilizer, (when present), optional buffer (when present), optional preservative (when present), and saline or water is 100 wt%. Even though the total wt% of the recited components is described as being 100, it will be understood by the skilled person that the pharmaceutical composition can comprise additional ingredients, components, and / or agents in the composition, including any of those described in this section “Additional Components of the Composition”

[00217] In some or any embodiments, the second agent can be formulated or packaged with the compound provided herein. Of course, the second agent will be formulated with the compound provided herein when, according to the judgment of those of skill in the art, such co-formulation should not interfere with the activity of either agent or the method of administration. In some or any embodiments, the compound provided herein and the second agent are formulated separately. They can be packaged together, or packaged separately, for the convenience of the practitioner of skill in the art.

[00218] In clinical practice the active agents provided herein may be administered by any conventional route, in particular parenterally, rectally, orally, by inhalation (e.g. in the form of aerosols), or topically.

[00219] The composition(s) of the present disclosure described herein can be administered by applying the composition(s) topically on the wound. If the composition is included in a medical device described herein which includes a substrate such as a patch or a pad, the medical device can be secured to the wound such that the composition contacts the wound.

[00220] Use may be made, as solid compositions for oral administration, of tablets, pills, hard gelatin capsules, powders, or granules. In these compositions, the active product is mixed with one or more inert diluents or adjuvants, such as sucrose, lactose, or starch.

[00221] These compositions can comprise substances other than diluents, for example a lubricant, such as magnesium stearate, or a coating intended for controlled release.

[00222] Use may be made, as liquid compositions for oral administration, of solutions which are pharmaceutically acceptable, suspensions, emulsions, syrups, and elixirs containing inert diluents, such as water or liquid paraffin. These compositions can also comprise substances other than diluents, in some or any embodiments, wetting, sweetening or flavoring products.

[00223] The compositions for parenteral administration can be emulsions or sterile solutions. Use may be made, as solvent or vehicle, of propylene glycol, a polyethylene glycol, vegetable oils, in particular olive oil, or injectable organic esters, in some or any embodiments, ethyl oleate. These compositions can also contain adjuvants, in particular wetting, isotonizing, emulsifying, dispersing and stabilizing agents. Sterilization can be carried out in several ways, in some or any embodiments, using a bacteriological filter, by radiation or by heating. They can also be prepared in the form of sterile solid compositions which can be dissolved at the time of use in sterile water or any other injectable sterile medium.

[00224] The compositions for rectal administration are suppositories or rectal capsules which contain, in addition to the active principle, excipients such as cocoa butter, semisynthetic glycerides or polyethylene glycols.

[00225] The compositions can also be aerosols. For use in the form of liquid aerosols or sprays, the compositions can be stable sterile solutions or solid compositions dissolved at the time of use in apyrogenic sterile water, in saline or any other pharmaceutically acceptable vehicle. For use in the form of dry aerosols intended to be directly inhaled, the active principle is finely divided and combined with a water-soluble solid diluent or vehicle, in some or any embodiments, dextran, mannitol or lactose. In one or more embodiments, a pharmaceutical composition provided herein is a spray.

[00226] In some or any embodiments, a composition provided herein is a pharmaceutical composition or a single unit dosage form. Pharmaceutical compositions and single unit dosage forms provided herein comprise a therapeutically effective amount of one or more therapeutic agents (e.g., a compound provided herein, or other therapeutic agent), and a typically one or more pharmaceutically acceptable carriers (e.g. excipients). In a specific embodiment and in this context, the term “pharmaceutically acceptable” means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans. In one or more embodiments, the term “carrier” includes a diluent, disintegrant, lubricant, adjuvant (e.g., Freund’s adjuvant (complete and incomplete)), excipient, or vehicle with which the therapeutic is administered. Such pharmaceutical carriers can be sterile liquids, such as water and oils, including those of petroleum, animal, vegetable, or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. Water can be used as a carrier when the pharmaceutical composition is administered intravenously. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers, particularly for injectable solutions. Examples of suitable pharmaceutical carriers are described in Remington: The Science and Practice of Pharmacy; Pharmaceutical Press; 22 edition (September 15, 2012).

[00227] Typical pharmaceutical compositions and dosage forms comprise one or more excipients. Suitable excipients are well-known to those skilled in the art of pharmacy, and in some or any embodiments, suitable excipients include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol, and the like. Whether a particular excipient is suitable for incorporation into a pharmaceutical composition or dosage form depends on a variety of factors well known in the art including, but not limited to, the way in which the dosage form will be administered to a mammal and the specific active ingredients in the dosage form. The composition or single unit dosage form, if desired, can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents.

[00228] Lactose free compositions provided herein can comprise excipients that are well known in the art and are listed, in some or any embodiments, in the U.S. Pharmacopeia (USP 36-NF 31 S2). In general, lactose free compositions comprise an active ingredient, a binder / filler, and a lubricant in pharmaceutically compatible and pharmaceutically acceptable amounts. Examples of lactose free dosage forms comprise an active ingredient, microcrystalline cellulose, pre gelatinized starch, and magnesium stearate.

[00229] Further provided are pharmaceutical compositions and dosage forms that comprise one or more compounds that reduce the rate by which an active ingredient will decompose. Such compounds, which are referred to herein as stabilizers, include, but are not limited to, antioxidants.

[00230] Further provided are pharmaceutical compositions and dosage forms that comprise one or more chemical permeation enhancer. In some or any embodiments, chemical permeation enhancers include but are not limited to one or more of ethanol, amides (such as Ozone, Laurocapram), alkyl and benzoate esters, fatty acid esters (such as isopropyl myristate, propylene glycol monocaprylate and propyleneglycomonolaurate), Transcutol (Registered name), fatty acids (oleic acid), glycols, pyrrolidone (N-methyl-2-pyrrolidone and 2-pyrrolidone, dimethylsulfoxide (DMSO), terpenes (such as essential oils comprising terpenes), phospholipids, and / or cyclodeextrines.

[00231] The pharmaceutical compositions and single unit dosage forms can take the form of solutions, suspensions, emulsion, sustained-release formulations, and the like. In one or more embodiments, the formulation suits the mode of administration. In a some or any embodiment, the pharmaceutical compositions or single unit dosage forms are sterile and in suitable form for administration to a mammal, in some or any embodiments, a human.

[00232] A pharmaceutical composition is formulated to be compatible with its intended route of administration. In some or any embodiments, routes of administration include, but are not limited to, parenteral, e.g., intrathecal, epidural, local or regional for peripheral nerve block, intravenous, intradermal, subcutaneous, intramuscular, subcutaneous, oral, buccal, sublingual, inhalation, intranasal, transdermal, topical (including administration to the eye, and in one or more embodiments to the cornea), transmucosal, intra-tumoral, intra-synovial, and rectal administration. In a specific embodiment, the composition is formulated in accordance with routine procedures as a pharmaceutical composition adapted for intravenous, subcutaneous, intramuscular, oral, intranasal, or topical (including administration to the eye, and in one or more embodiments to the cornea) administration to human beings. In a specific embodiment, a pharmaceutical composition is formulated in accordance with routine procedures for subcutaneous administration to human beings. Typically, compositions for intravenous administration are solutions in sterile isotonic aqueous buffer. The composition may also include one or more solubilizers and a local anesthetic such as lignocamne to ease pain at the site of the injection.

[00233] In some or any embodiments, dosage forms include, but are not limited to: sprays, tablets; caplets; capsules, such as soft elastic gelatin capsules; cachets; troches; lozenges; dispersions; suppositories; ointments; cataplasms (poultices); pastes; powders; dressings; creams; plasters; solutions; patches; aerosols (e.g., nasal sprays or inhalers); gels; liquid dosage forms suitable for oral or mucosal administration to a mammal, including suspensions (e.g., aqueous or non-aqueous liquid suspensions, oil in water emulsions, or a water in oil liquid emulsions), solutions, and elixirs; liquid dosage forms suitable for parenteral administration to a mammal; and sterile solids (e.g., crystalline or amorphous solids) that can be reconstituted to provide liquid dosage forms suitable for parenteral administration to a mammal.

[00234] The composition, shape, and type of dosage forms provided herein will typically vary depending on their use. In some or any embodiments, a dosage form used in the initial treatment of the disease, disorder, or condition may contain larger amounts of one or more of the active ingredients it comprises than a dosage form used in the maintenance treatment of the same disease, disorder, or condition. These and other ways in which specific dosage forms encompassed herein will vary from one another will be readily apparent to those skilled in the art. See, e.g.. Remington: The Science and Practice of Pharmacy; Pharmaceutical Press; 22 edition (September 15, 2012). Glycol

[00235] In one or more embodiments, the pharmaceutical composition comprises a glycol. In one or more embodiments, the glycol is a non-geminal diol or a geminal diol. In one or more embodiments, the glycol is a 3- or 4-carbon glycol. In one or more embodiments, the glycol is a propanediol, such as propylene glycol (for example, 1,2-propanediol). In one or more embodiments, the glycol is a butanediol, such as butylene glycol (for example, 1,3-butanediol). In one or more embodiments, the glycol is a polyethylene glycol. The glycol can be PEG-400 (polyethylene glycol 400), but is not limited thereto.

[00236] In one or more embodiments, the glycol is unconjugated to another molecule. For example, the glycol may be a 3- or 4-carbon glycol or a PEG, without further conjugation to another molecule.

[00237] Examples of conjugated glycols include, but are not limited to, glycol stearate and glycol laurate. As described in the following section, a conjugated glycol such as PEG-400 monostearate may be a solubilizer.

[00238] In one or more embodiments, the glycol is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 20 wt% of that of the total composition. For example, the glycol can be from about 0.2 wt% to about 10 wt%, from about 0.5 wt% to about 10 wt%, from about 1 wt% to about 10 wt%, from about 2 wt% to about 10 wt%, from about 3 wt% to about 10 wt%, from about 3 wt% to about 9 wt%, from about 3 wt% to about 8 wt%, from about 3 wt% to about 7 wt%, from about 3 wt% to about 6 wt%, or from about 4 wt% to about 6 wt% of the total composition. In one or more embodiments, the glycol is present in the pharmaceutical composition in an amount from about 2 wt% to about 10 wt%, preferably from about 3 wt% to about 5 wt% or from about 3 wt% to about 6 wt% or from about 4 wt% to about 5 wt% or about 4.6 wt%.

[00239] In some or any embodiments, the glycol is present in the pharmaceutical composition in an amount from about 2 wt% to less than 8 wt%; in some embodiments when the wound is a surface wound.

[00240] In some or any embodiments, the glycol is present in the pharmaceutical composition in an amount from about 2 wt% to about 10 wt%, in some embodiments, 8 wt% to about 10 wt%; in some embodiments when the wound is a bum wound where higher hydration may be desired.

[00241] The glycol can be present in the pharmaceutical composition in a concentration from about 0.05 mg / mL to about 100 mg / mL, from about 1 mg / mL to about 100 mg / mL, from about 10 mg / mL to about 100 mg / mL, from about 20 mg / mL to about 100 mg / mL, from about 30 mg / mL to about 100 mg / mL, from about 40 mg / mL to about 100 mg / mL, from about 1 mg / mL to about 90 mg / mL, from about 10 mg / mL to about 90 mg / mL, from about 20 mg / mL to about 90 mg / mL, from about 30 mg / mL to about 90 mg / mL, from about 40 mg / mL to about 90 mg / mL, from about 1 mg / mL to about 80 mg / mL, from about 10 mg / mL to about 80 mg / mL, from about 20 mg / mL to about 80 mg / mL, from about 30 mg / mL to about 80 mg / mL, from about 40 mg / mL to about 80 mg / mL, from about 1 mg / mL to about 70 mg / mL, from about 10 mg / mL to about 70 mg / mL, from about 20 mg / mL to about 70 mg / mL, from about 30 mg / mL to about 70 mg / mL, from about 40 mg / mL to about 70 mg / mL, or from about 41 mg / mL to about 66 mg / mL. Solubilizer

[00242] In one or more embodiments, the one or more solubilizers comprise: PPG-26 Buteth-26, PEG-40 hydrogenated castor oil (INCI name), and water; or the one or more solubilizers are one or more selected from the group consisting of hydroxypropyl cellulose, polysorbate 20, polysorbate 40, polysorbate 60, and polysorbate 80. In one or more embodiments, the one or more solubilizers comprise Solubilisant LRI® (Sensient Cosmetic Technologies, Saint Ouen L’ Aumone, France) or the one or more solubilizers are one or more selected from the group consisting of hydroxypropyl cellulose, polysorbate 20, polysorbate 40, polysorbate 60, and polysorbate 80.

[00243] When the one or more solubilizer comprises PPG-26 Buteth-26, PEG-40 hydrogenated castor oil, and water, the one or more solubilizer allows water to be a substitute for alcohol. A suitable example includes, but is not limited to, Solubilisant LRI® (Sensient Cosmetic Technologies, Saint Ouen L’ Aumone, France).

[00244] PPG-26-Buteth-26 is a polyoxypropylene, polyoxyethylene ether of butyl alcohol, where PPG stands for polypropylene glycol. Suitable examples include, but are not limited to, Chemical Abstracts Service (“CAS”) Registry Number 9065-63-8. In the CAS registry, PPG-26 Buteth-26 may also be called polyoxyethylene (26) polyoxypropylene (26) monobutyl ether, and polyoxypropylene (26) polyoxyethylene (26) monobutyl ether, and has the following structure:                  m          , where m is an integer representing a number of polyoxypropylene subunit, n is an integer representing a number of polyoxyethylene subunit, where n and m are included in the molecular weight. The number average molecular weight (Mn) may be about 3,900 g / mol (3,900 Daltons).

[00245] PEG-40 hydrogenated castor oil is a polyethylene derivative of castor oil. A suitable example of PEG-40 hydrogenated castor oil includes, but is not limited to, CAS Reg. No. 61788-85-0. PEG-40 hydrogenated castor oil may include polyethyleneglycol 400 (PEG-400) monostearate (a conjugated PEG).

[00246] In one or more embodiments, the one or more solubilizers can be one or two solubilizers, or two solubilizers.

[00247] In one or more embodiments, the one or more solubilizers is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 20 wt % of that of the total composition. For example, the one or more solubilizers can be from about 0.2 wt% to about 10 wt%, or from about 0.5 wt% to about 10 wt%, or from about 1 wt% to about 10 wt%, or from about 1 wt% to about 9 wt%, or from about 1 wt% to about 8 wt%, or from about 1 wt% to about 7 wt%, or from about 1 wt% to about 6 wt%, or from about 2 wt% to about 10 wt%, or from about 2 wt% to about 8 wt%, or from about 2 wt% to about 6 wt% of the total composition. In one or more embodiments, the pharmaceutical composition comprise 3 wt% to 5 wt% or 2 wt% to 6 wt% of the one or more solubilizer (ex: 4.6% polysorbate 20, polysorbate 60, polysorbate 80, or solubilisant™).

[00248] The one or more solubilizer can be present in the pharmaceutical composition in a concentration from about 0.5 mg / mL to about 100 mg / mL, from about 1 mg / mL to about lOOmg / mL, from about 1 mg / mL to about 90 mg / mL, from about 1 mg / mL to about 80 mg / mL, from about 1 mg / mL to about 70 mg / mL, from about 5 mg / mL to about lOOmg / mL, from about 5 mg / mL to about 90 mg / mL, from about 5 mg / mL to about 80 mg / mL, from about 5 mg / mL to about 70 mg / mL, from about 6 mg / mL to about 70 mg / mL, or from about 6 mg / mL to about 66 mg / mL. Phospholipid

[00249] In one or more embodiments, the one or more phospholipid can be phosphatidylcholine or lecithin. In one or more embodiments, the one or more phospholipid is phosphatidylcholine. In one or more embodiments, the one or more phospholipid is lecithin.

[00250] In one or more embodiments, the phosphatidylcholine is derived from soy (CAS Reg. No. 97281-47-5). In one or more embodiments, the phosphatidylcholine is a phosphatidylcholine mixture containing phosphatidylcholine in an amount from 90 wt% or greater, 91 wt% or greater, 92 wt% or greater, 93 wt% or greater, 94 wt% or greater, or greater than or equal to 94 wt%. A suitable example of the phosphatidylcholine mixture includes, but is not limited to, Phospholipon® 90G (Lipoid, Ludwigshafen / Rhine, Germany). Phospholipon® 90G is a mixture of >94 wt% phosphatidylcholine, with the remaining amount of the phosphatidylcholine mixture containing ascorbyl palmitate (CAS Reg. No. 137-66-6) and tocopherol (CAS Reg. No. 10191-41-0, (±)-a-Tocopherol, DL-all-rac-a-Tocopherol, Vitamin E).

[00251] In one or more embodiments, the one or more phospholipid is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 20 wt% of that of the total composition. For example, the one or more phospholipid can be from about 0.1 wt% to about 15 wt%, or from about 0.1 wt% to about 10 wt%, or from about 0.1 wt% to about 9 wt%, or from about 0.1 wt% to about 8 wt%, or from about 0.1 wt% to about 7 wt%, or from about 0.1 wt% to about 6 wt%, or from about 0.1 wt% to about 5 wt%, or from about 0.1 wt% to about 4 wt%, or from about 0.1 wt% to about 3 wt%, or from about 0.1 wt% to about 2 wt%, or from about 0.1 wt% to about 1 wt% of the total composition. In one or more embodiments, the one or more phospholipid is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 1.2 wt%. In one or more embodiments, the pharmaceutical composition comprises 0.1% to 2wt% of the one or more phospholipid. In one or more embodiments, the pharmaceutical composition comprises 1.2% of the one or more phospholipid. Carrier

[00252] In one or more embodiments, the one or more carrier is a C2-C4 alcohol. In one or more embodiments, the carrier is a C2-C3 alcohol. In one or more embodiments, the one or more carrier is ethanol.

[00253] In one or more embodiments, the one or more carrier is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 10 wt% of that of the total composition. For example, the one or more carrier can be from 1 wt% to 10 wt%, or from about 1 wt% to about 9 wt%, or from about 1 wt% to about 8 wt%, or from about 1 wt% to about 7 wt%, or from about 1 wt% to about 6 wt%, or from about 2 wt% to about 10 wt%, or from about 2 wt% to about 9 wt%, or from about 2 wt% to about 8 wt%, or from about 2 wt% to about 7 wt%, or from about 2 wt% to about 6 wt%, or from about 3 wt% to about 10 wt%, or from about 3 wt% to about 9 wt%, or from about 3 wt% to about 8 wt%, or from about 3 wt% to about 7 wt%, or from about 3 wt% to about 6 wt%, or from about 4 wt% to about 5 wt% of the total composition. Stabilizer

[00254] In a pharmaceutical composition that includes GO-HA and a Wnt inhibitor, the inventors surprisingly discovered that GO-HA can oxidize a WNT inhibitor in some instances. For example, when GO-HA and XAV939 present in a pharmaceutical composition, such as a liposomal composition (in some embodiments a water-based liposomal composition), the XAV939 was oxidized by the GO-HA. The skilled person could not have predicted this based on the state of the art. Advantageously, the addition of a stabilizer in a pharmaceutical composition according to one or more embodiments prevents oxidation of a Wnt inhibitor, such as XAV939.

[00255] In one or more embodiments, the stabilizer can be an antioxidant. In one or more embodiments, the stabilizer is an amino acid. In one or more embodiments, the stabilizer is or comprises a sulfur-containing amino acid. Suitable stabilizers include, but are not limited to, methionine, cysteine, cystine, taurine, and glutathione. In one or more embodiments, the stabilizer is methionine.

[00256] In one or more embodiments, the stabilizer is present in the pharmaceutical composition in an amount from about 0.05 wt% to about 3 wt% of that of the total composition. For example, the stabilizer can be from about 0.05 wt% to about 2 wt%, or from about 0.05 wt% to about 1 wt%, or from about 0.05 wt% to about 0.5 wt%, or from about 0.1 wt% to about 3 wt%, or from about 0.1 wt% to about 2 wt%, or from about 0.1 wt% to about 1 wt%, or from about 0.1 wt% to about 0.5 wt%, or from about 0.1 wt% to about 0.4 wt%, or from about 0.1 wt% to about 3 wt%, or from about 0.1 wt% to about 0.2 wt%, or from about 0.2 wt% to about 0.5 wt%, or from about 0.3 wt% to about 0.5 wt%, or from about 0.4 wt% to about 0.5 wt%, or from about 0.3 wt% to about 0.4 wt%, or from about 0.3 wt% to about 0.5 wt% of the total composition. In one or more embodiments, the stabilizer is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 0.5 wt%. Buffer

[00257] In one or more embodiments, the buffer is or comprises a C4-C8 organic acid. In one or more embodiments, the buffer is or comprises a Ce organic acid. The buffer can be a weak acid. A suitable example of the buffer is or comprises citric acid. In one or more embodiments, the buffer is a C4-C8 organic acid. In one or more embodiments, the buffer is a Ce organic acid. The buffer is a weak acid. A suitable example of the buffer is citric acid. In one or more embodiments, the buffer comprises a C4-C8 organic acid. In one or more embodiments, the buffer comprises a Ce organic acid. The buffer comprises a weak acid. A suitable example of the buffer comprises citric acid.

[00258] A buffer can be added to maintain the pH of the pharmaceutical composition. The buffer can be sufficient to maintain an acidic or neutral pH. The buffer can be sufficient to maintain a pH of the pharmaceutical composition in a range from about 3 to about 7, such as from about 3.5 to about 7, from about 4 to about 7, from about 4.5 to about 7, from about 5 to about 7, from about 5.5 to about 7, from about 6 to about 7, from about 6.5 to about 7, from about 3 to about 6.5, from about 3.5 to about 6.5, from about 4 to about 6.5, from about 4.5 to about 6.5, from about 5 to about 6.5, from about 5.5 to about 6.5, from about 6 to about 6.5, from about 3 to about 6, from about 3.5 to about 6, from about 4 to about 6, from about 4.5 to about 6, from about 5 to about 6, from about 5.5 to about 6, from about 3 to about 5.5, from about 3.5 to about 5.5, from about 4 to about 5.5, from about 4.5 to about 5.5, from about 5 to about 5.5, from about 3 to about 5, from about 3.5 to about 5, from about 4 to about 5, from about 4.5 to about 5, from about 3 to about 4.5, from about 3.5 to about 4.5, from about 4 to about 4.5, from about 3 to about 4, from about 3.5 to about 4, or from about 3 to about 3.5. In one or more embodiments, the pharmaceutical composition pH is about 3, about 3.5, about 4, about 4.5, about 5, about 5.5, about 6, about 6.5, or about 7.

[00259] In one or more embodiments, the buffer is present in the pharmaceutical composition in an amount from about 0.1 wt% to about 20 wt% of that of the total composition. For example, the buffer can be from about 0.1 wt% to about 10 wt%, or from about 0.1 wt% to about 8 wt%, or from about 0.1 wt% to about 6 wt%, or from about 0.1 wt% to about 4 wt%, or from about 0.1 wt% to about 3 wt%, or from about 0.1 wt% to about 2 wt%, or from about 0.1 wt% to about 1 wt%, or from about 0.2 wt% to about 10 wt%, or from about 0.2 wt% to about 8 wt%, or from about 0.2 wt% to about 6 wt%, or from about 0.2 wt% to about 4 wt%, or from about 0.2 wt% to about 3 wt%, or from about 0.2 wt% to about 2 wt%, or from about 0.2 wt% to about 1 wt% of the total composition. In one or more embodiments, the stabilizer is present in the pharmaceutical composition in an amount from about 0.2 wt% to about 2.0 wt%. Preservative

[00260] A preservative can be included in the pharmaceutical composition to prevent growth of bacteria, mold, yeast, and / or fungi. In one or more embodiments, the preservative comprises phenoxyethanol and ethylhexylglycerin, or is potassium sorbate.

[00261] When the preservative comprises phenoxyethanol and ethylhexylglycerin, the phenoxyethanol may be in an amount from about 85 wt% to 90 wt% and the phenoxyethanol may be in an amount from about 7 wt% to 13 wt% of the total preservative wt %. A suitable example of the preservative comprising phenoxyethanol and ethylhexylglycerin is Euxyl® PE 9010 (Ashland Global, Wilmington, Del eware, USA). The phenoxyethanol may be 2-phenoxyethanol. The ethylhexylglycerin may be 3-[(2-ethylhexyl)oxy]-l,2-propanediol.

[00262] In one or more embodiments, the preservative is potassium sorbate. In one or more embodiments, the preservative is potassium sorbate in an amount from 0.5 wt% to 1.5 wt% of the total composition wt%.

[00263] In one or more embodiments, the preservative is Euxyl® PE 9010. In one or more embodiments, the preservative is Euxyl® PE 9010 in an amount from 0.5 wt% to 1.0 wt% of the total composition wt%.

[00264] In one or more embodiments, the preservative is present in the pharmaceutical composition in an amount from about 0.01 wt% to about 5 wt% of that of the total composition. For example, the preservative can be from about 0.01 wt% to about 4 wt%, or from about 0.01 wt% to about 3 wt%, or from about 0.01 wt% to about 2 wt%, or from about 0.01 wt% to about 1.5 wt%, or from about 0.01 wt% to about 1 wt%, or from about 0.01 wt% to about 0.5 wt%, or from about 0.025 wt% to about 4 wt%, or from about 0.025 wt% to about 3 wt%, or from about 0.025 wt% to about 2 wt%, or from about 0.025 wt% to about 1.5 wt%, or from about 0.025 wt% to about 1 wt%, or from about 0.025 wt% to about 0.5 wt%, or from about 0.5 wt% to about 5 wt%, or from about 0.5 wt% to about 4 wt%, or from about 0.5 wt% to about 3 wt%, or from about 0.5 wt% to about 2.5 wt%, or from about 0.5 wt% to about 2 wt%, or from about 0.5 wt% to about 1.5 wt%, or from about 0.5 wt% to about 1 wt%, of the total composition wt%. In one or more embodiments, the preservative can be from 0.5 wt% to 1.5 wt%, or from 0.5 wt% to 1.0 wt% of the total composition wt%. Thickener

[00265] In one or more embodiments, the pharmaceutical composition further comprises one or more thickener. The one or more thickener contributes a congealing effect on the composition based on its concentration in the total composition and its critical temperature, among other things. The one or more thickener provides desired viscosity of the composition for certain skin delivery. For example, the one or more thickener can make the composition into a smooth film for easy application. It also reduces evaporation and allows the wound to stay moist longer, a factor that has been shown to improve healing and result in decreased scarring. The one or more thickener can be an aqueous based thickener. A suitable example of an aqueous based thickener is hydroxypropyl methyl cellulose (HPMC or hypromellose): OR p, where R is -H, -CH3, or -CH2CH(OH)CH3, and p ranges from about 50,000 to 200,000 Mw. Hydroxypropyl methyl cellulose can also be called hydroxypropyl cellulose (or HPC). Another example of a thickener is xanthum gum.

[00266] For example, the one or more thickener can have a molecular weight range from 50,000 to 200,000 MW, or from about 60,000 MW to about 200,000 MW, or from about 80,000 to about 200,000 MW, or from about 90,000 to about 200,000 MW, or from about 50,000 to about 150,000 MW, or from about 60,000 to about 150,000 MW, or from about 70,000 to about 150,000 MW, or from about 80,000 to about 150,000 MW, or from about 50,000 to about 150,000 MW, or from about 70,000 to about 150,000 MW, or from about 90,000 to about 100,000 MW, or from about 60,000 to about 100,000 MW, or from about 80,000 to about 150,000 MW, or from about 90,000 to about 150,000 MW, or from about 60,000 to about 150,000 MW, or from about 80,000 to about 150,000 MW, or from about 50,000 to about 100,000 MW, or from about 70,000 to about 100,000 MW, or from about 90,000 to about 100,000 MW, or is about 90,000 MW, or is 90,000 MW. There are different grades of thickener available according to molecular weights of said thickener, or viscosity of thickener water solution(s) depending on thickener concentration.

[00267] In one or more embodiments, the one or more thickeners can be one or two thickeners, or two thickeners.

[00268] In one or more embodiments, the pharmaceutical composition does not comprise the one or more thickener. In some or more embodiments, the pharmaceutical composition does not comprise the one or more thickener and the pharmaceutical composition is a spray formulation. Saline or water

[00269] In one or more embodiments, the pharmaceutical composition comprises saline or water. The saline or water may be USP sterile grade, containing no antimicrobial agents. In one or more embodiments, the saline is from about 0.1 wt% to 1.0 wt% sodium chloride (NaCl) in water. For example, the saline can be from about 0.15 wt% to about 0.95 wt%, from about 0.20 wt% to about 0.90 wt%, from about 0.22 wt% to about 0.9 wt%, from about 0.3 wt% to about 0.9 wt%, from about 0.4 wt% to about 0.9 wt%, from about 0.5 wt% to about 0.9 wt%, from about 0.6 wt% to about 0.9 wt%, from about 0.7 wt% to about 0.9 wt%, or from about 0.8 wt% to about 0.9 wt% NaCl in water (Normal saline, USP sterile grade). In one or more embodiments, the saline is from about 0.22 wt% to about 0.9 wt% NaCl in water. In one or more embodiments, the saline is about 0.9 wt% NaCl in water, or 0.9 wt% NaCl in water. When all components, including additional active agents and unspecified additional components, of the pharmaceutical composition are accounted for in wt% of the composition except saline or water, the remaining wt% amount of the composition is saline or water. Additional weight ratios (wt%)

[00270] In one or more embodiments, the wt% ratio of WNT inhibitor:one or more phospholipid in the pharmaceutical composition is from about 1:1 to about 1:50. For example, the wt% ratio of WNT inhibitor:one or more phospholipid in the pharmaceutical composition can be from about 1:5 to about 1:50, from about 1:10 to about 1:50, from about 1:15 to about 1:50, from about 1:20 to about 1:50, from about 1:25 to about 1:50, from about 1:30 to about 1:50, from about 1:35 to about 1:50, from about 1:40 to about 1:50, or from about 1:45 to about 1:50. In some embodiments, the WNT inhibitor is XAV939, Compound 1, Compound 7, Compound 8, Compound 10, or Compound 18. In some embodiments, the WNT inhibitor is XAV939.

[00271] In one or more embodiments, the wt% ratio of WNT inhibitor:one or more phospholipid in the pharmaceutical composition is from about 1:10 to about 1:30. For example, the wt% ratio of WNT inhibitor:one or more phospholipid in the pharmaceutical composition is from about 1:1 to about 1:50, from about 1:1 to about 1:25, from about 1:10 to about 1:25, from about 1:10 to about 1:24, from about 1:10 to about 1:23, from about 1:10 to about 1:22, from about 1:10 to about 1:21, from about 1:10 to about 1:20, from about 1:10 to about 1:19, from about 1:10 to about 1:18, from about 1:10 to about 1:17, from about 1:10 to about 1:16, from about 1:10 to about 1:15, from about 1:10 to about 1:14, from about 1:10 to about 1:13, from about 1:10 to about 1:12, or from about 1:10 to about 1:11. In some or any embodiments, the WNT inhibitor is XAV939, Compound 1, Compound 7, Compound 8, Compound 10, or Compound 18. In some or any embodiments, the WNT inhibitor is XAV939.

[00272] In one or more embodiments of the pharmaceutical composition comprising one or more phospholipid, the wt% ratio of the Wnt inhibitor, such as a Wnt inhibitor of Formula (I) (for example, XAV939, Compound 1, Compound 7, Compound 8, Compound 10, and Compound 18) to the one or more phospholipid or the one or more phospholipid to the WNT inhibitor can be about 1:1, about 1:1.1, about 1:1.2, about 1:1.3, about 1:1.4, about 1:1.5, about 1:1.6, about 1:1.7, about 1:1.8, about 1:1.9, or about 1:2.

[00273] In one or more embodiments, the wt% of the one or more carrier(s):one or more phospholipid in the pharmaceutical composition is from about 1:1 to about 10:1. For example, the wt% ratio of the one or more carrier(s):one or more phospholipid in the pharmaceutical composition is from about 2:1 to about 10:1, from about 2:1 to about 9:1, from about 2:1 to about 9:1, from about 2:1 to about 8:1, from about 2:1 to about 7:1, from about 2:1 to about 6:1, from about 2:1 to about 5:1, from about 2:1 to about 4:1, from about 2:1 to about 3:1, from about 3:1 to about 10:1, from about 3:1 to about 9:1, from about 3:1 to about 8:1, from about 3:1 to about 7:1, from about 3:1 to about 6:1, from about 3:1 to about 5:1, or from about 3:1 to about 4:1.

[00274] In one or more embodiments, the wt% of the glycol:one or more solubilizer (solubilizers) are present in a wt% ratio in the pharmaceutical composition of about 1:0.1 to about 1:5. For example, the wt% of the glycokone or more solubilizer in the pharmaceutical composition is from about 1:0.1 to about 1:4, from about 1:0.1 to about 1:3, from about 1:0.1 to about 1:2, from about 1:0.1 to about 1:1, from about 1:0.25 to about 1:5, from about 1:0.25 to about 1:4, from about 1:0.25 to about 1:3, from about 1:0.25 to about 1:2, from about 1:0.25 to about 1:1, from about 1:0.5 to about 1:5, from about 1:0.5 to about 1:4, from about 1:0.5 to about 1:3, from about 1:0.5 to about 1:2, from about 1:0.5 to about 1:1.5, or from about 1:0.5 to about 1:1.

[00275] In one or more embodiments, the wt% of the glycol: carrier (or carriers) are present in a wt% ratio in the pharmaceutical composition of about 1:0.1 to about 1:5. For example, the wt% of the glycol:carrier (or carriers) is from about 1:0.1 to about 1:4, from about 1:0.1 to about 1:3.5, from about 1:0.1 to about 1:3, from about 1:0.1 to about 1:2.5, from about 1:0.1 to about 1:2, from about 1:0.1 to about 1:1.5, from about 1:0.1 to about 1:1, from about 1:0.25 to about 1:4, from about 1:0.25 to about 1:3.5, from about 1:0.25 to about 1:3, from about 1:0.25 to about 1:2.5, from about 1:0.25 to about 1:2, from about 1:0.25 to about 1:1.5, from about 1:0.25 to about 1:1, from about 1:0.5 to about 1:4, from about 1:0.5 to about 1:3.5, from about 1:0.5 to about 1:3, from about 1:0.5 to about 1:2.5, from about 1:0.5 to about 1:2, from about 1:0.5 to about 1:1.5, or from about 1:0.5 to about 1:1.

[00276] In one or more embodiments, the phrase “wherein the weight% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more optional phospholipids, one or more optional carriers, an optional stabilizer, optional buffer, optional preservative, and water or saline is 100 wt%” does not exclude additional components being present in the composition. Topical Administration

[00277] The pharmaceutical composition can be formulated for application to surfaces of the body, such as to the skin or mucous membranes. Thus, the pharmaceutical composition can be a topical, formulated for topical administration. Pharmaceutical compositions according to one or more embodiments herein can be applied directly to the skin, epicutaneously.

[00278] In one or more embodiments, the pharmaceutical composition is a topical solution. Suitable topical solutions include, but are not limited to, a drop, a rinse, and a spray. Examples include a serum (such as a skin serum), an eye drop, an ear drop, and an inhalant. The topical solution may be of lower viscosity compared with other topicals, including but not limited to creams and gels.

[00279] When the pharmaceutical composition is a spray, it may be applied (sprayed) directly to the target site. The spray may be configured to pass through a spray nozzle, atomizer, or orifice, which fragments the liquid into smaller liquid structures such a sheets, ligaments, streams, droplets, or combinations thereof. The spray may be configured for atomization, where the spray includes atomizing air passing through the stream of the spray resulting in fine liquid droplets, which is applied to the target site. The spray may be configured for vaporization, where the spray is a vapor, which is applied to the target site. The spray may be configured as an aerosol, where liquid droplets are suspended in air or another suitable gas, which is applied to the target site.

[00280] When topically administered, the pharmaceutical composition can provide a faster onset and more concentrated local effect on the target site than with other forms of administration. As a topical, the pharmaceutical composition bypasses the first-pass effect (first-pass metabolism), thereby preventing loss of individual components, such as an active ingredient or drug, through sites of elimination during absorption. In other words, the pharmaceutical composition can bypass first-pass metabolism of the liver, intestinal lumen, or gut wall enzymes. Advantageously, the pharmaceutical composition as a topical retains the components of the composition during and after application to the extent that the effects of the pharmaceutical composition are realized, without being metabolized or separated.

[00281] Although permeability of skin varies with condition, anatomic site, age, and gender, benefits of the pharmaceutical composition as a topical (or as topically administered) include slow absorption through the skin, so the pharmaceutical composition remains at the target site. In the event of misapplication, the pharmaceutical composition can be washed off, thereby preventing unwanted side effects. By application to the target site, proprioception of the subject can be improved compared to other forms of administration.

[00282] Advantageously, the pharmaceutical composition according to one or more embodiments herein can prevent infection, prevent sunburn, reduce inflammation, and induce insensitivity to pain (anesthetic), each without causing rashes or itchiness, compared to without the pharmaceutical composition according to one or more embodiments.

[00283] Additional benefits of the topical composition and topical administration of embodiments of the present disclosure is low toxicity and high bioavailability of the beneficial components to the site of injury / wound. Methods

[00284] The methods provided herein encompass administering pharmaceutical compositions containing one or more compound as described herein, including a Wnt Inhibitor of Formula (I) if appropriate in a salt form, either used alone or in the form of a combination with one or more compatible and pharmaceutically acceptable carriers, such as diluents or adjuvants, or with another agent for the treatment of wounds and / or conditions modulated by Wnt transcription products or Wnt signaling pathway activity.

[00285] In one or more embodiments, provided is a method for inhibiting Wnt transcription products or Wnt signaling pathway activity in a mammal comprising contacting the subject with an effective amount of a pharmaceutical composition according to one or more embodiments herein.

[00286] In one or more embodiments, provided is a method for treating a disease, disorder, or condition associated with Wnt transcription products or Wnt signaling pathway activity in a mammal in need thereof, comprising administering a pharmaceutical composition according to one or more embodiments herein.

[00287] In one or more embodiments, provided is a method for stimulating regeneration of tissue at a wound in a mammal in need thereof and wherein the wound is contacted with a pharmaceutical composition according to one or more embodiments herein.

[00288] In one or more embodiments, provided is a method for administering topically a pharmaceutical composition according to one or more embodiments herein, to a mammal in need thereof.

[00289] In one or more embodiments, a disease, disorder, or condition (to be treated in one or more embodiments of a method herein) is a chronic wound, an acute wound, an alkali-burned corneal wound, a burn, a lesion (including lesions caused by HPV and / or a virus selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), a cartilage disease (including osteoarthritis, rheumatoid arthritis, internal derangement of the joints, and degenerative cartilage disease), a bone disease (including osteoporosis), organ fibrosis (including lung fibrosis, heart fibrosis, liver fibrosis, and kidney fibrosis), cancer (including melanoma, breast cancer, and prostate cancer), a denerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, tissue in need of neovascularization, osteoclast differentiation in need of inhibition, osteoblast differentiation disorders (where inhibition of osteoblast differentiation is needed), and / or bone destruction associated with breast cancer.

[00290] In one or more embodiments, a disease, disorder, or condition (to be treated in one or more embodiments of a method herein) is a chronic wound, an acute wound, an alkali-burned corneal wound, an incisional wound (open or closed), a burn, a lesion (including lesions caused by HPV and / or a virus selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), a cartilage disease (including osteoarthritis, rheumatoid arthritis, internal derangement of the joints, and degenerative cartilage disease), a bone disease (including osteoporosis), organ fibrosis (including lung fibrosis, heart fibrosis, liver fibrosis, and kidney fibrosis), cancer (including melanoma, breast cancer, and prostate cancer), a de-nerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, tissue in need of neovascularization, osteoclast differentiation in need of inhibition, osteoblast differentiation disorders (where inhibition of osteoblast differentiation is needed), and / or bone destruction associated with breast cancer.

[00291] In one or more embodiments, a disease, disorder, or condition (to be treated in one or more embodiments of a method herein) is selected from a chronic wound, an acute wound, an alkali-burned corneal wound, an incisional wound (open or closed), a bum, a lesion (including lesions caused by HPV and / or a vims selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), cancer (including melanoma and breast cancer), a de-nerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, and tissue in need of neovasculari zati on.

[00292] In one or more embodiments, provided is a method of inducing bacteriostasis associated with Wnt transcription products or Wnt signaling pathway activity, comprising administering a pharmaceutical composition according to one or more embodiments herein to a mammal in need thereof.

[00293] In one or more embodiments, provided is a method for treating an open excisional wound in a mammal in need thereof, comprising administering a pharmaceutical composition according to one or more embodiments herein.

[00294] In one or more embodiments, provided is a method for treating a closed excisional wound in a mammal in need thereof, comprising administering a pharmaceutical composition according to one or more embodiments herein.

[00295] In one or more embodiments, provided is a method for treating a bum in a mammal in need thereof, comprising administering a pharmaceutical composition according to one or more embodiments herein. Synthetic Examples Compound 1 Synthesis of (4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 1) O OH Synthesis of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one

[00297] A mixture of 4-bromobenzimidamide hydrochloride (1) (22.0 g, 93.41 mmol, 1.0 eq), methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1.1 g, 102.76 mmol, 1.1 eq), and K2CO3 (38.73 g, 280.23 mmol, 3.0 eq) in MeOH (660 mL) was stirred at 80°C for 16 h. The mixture was cooled and concentrated in vacuo. The solid was triturated with water (500 mL), and then filtered and dried to afford 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (26.4 g, 81.7 mmol). LC-MS: calculated for Ci3HnBrN2OS: 323.2; found: 325.0. Synthesis of 2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one

[00299] The degassed mixture of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (20 g, 61.88 mmol, 1.0 eq), (BPin)2 (31.43 g, 123.76 mmol, 2.0 eq), Pd(dppf)C12 (2.26 g, 3.09 mmol, 0.05 eq), and KOAc (30.36 g, 309.40 mmol, 5.0 eq) in dioxane (200 mL) was stirred at 100°C for 16 h under Argon. The mixture was concentrated in vacuo. The crude product was purified on a silica gel column (PE:EtOAc = 5:1 to 1:1) to afford 2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (21.73 g, 58.72 mmol). LC-MS: calculated for C19H23BN2O3S: 370.1; found: 371.1.

[00300] Scheme IC

[00301] A solution of 2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (10.0 g, 370.27 mmol, 1.0 eq) in 3M HCl / MeOH (100 mL) was stirred at 25°C for 16 h. The mixture was concentrated under vacuum to afford a crude product which was triturated with THF (150 mL) under reflux and filtered to afford (4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 1). LC-MS: calculated for C13H13BN2O3S: 288.1; found: 289.1. 'H NMR (400 MHz, MeOD): 5 7.95 (s, 4H), 3.67 (s, 2H), 3.10 (m, 2H), 3.02 (m, 2H). Compound 2 Synthesis of 2-oxo-l-((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl) cyclobutyl)-2,3-dihydro-lH-benzo[d]imidazole-5-carbonitrile (Compound 2) O CN Synthesis of (Z)-tert-butyl (3-hydroxycyclobutyl)carbamate

[00302] Scheme 2A H 1 L-Selectride THF, -78°C, 2 h 2

[00303] A solution of tert-butyl (3-oxocyclobutyl)carbamate (1) (2.5 g, 13.5 mmol, 1.0 eq) in 80 mL THF was cooled to -78°C and treated with a 1 N solution of L-Selectride (16.2 mL, 16.2 mmol, 1.2 eq) in THF. After stirring for 1 h the reaction was quenched with 5 mL water and warmed to rt. The reaction mixture was concentrated and purified by silica gel chromatography (EA:PE =     0-50%) provided the (Z)-tert-butyl (3-hydroxycyclobutyl)carbamate (2) (1.8 g). ^NMR (400 MHz, CDCh): 5 (ppm): 4.68 (brs, 1H), 4.05-3.98 (m, 1H), 3.67-3.65 (m, 1H), 2.78-2.75 (m, 2H), 2.08 (brs, 1H), 1.81-1.78 (m, 2H), 1.44 (s, 9H). Synthesis of (Z)-3-((tert-butoxycarbonyl)amino)cyclobutyl me thane sulfonate

[00304] Scheme 2B MsCI, TEA Ms° Boc              - ^N'Boc H          DCM, -70°C, 2 h                 H cis                                                     cis 2                                           3

[00305] Methanesulfonyl chloride (1.3 g, 11.6 mmol, 1.2 eq) was added dropwise to a -70°C solution of (Z)-tert-butyl (3-hydroxycyclobutyl)carbamate (2) (1.8 g, 9.6 mmol, 1.0 eq) and TEA (1.5 g, 14.8 mmol, 1.5 eq) in dichloromethane (60 mL). The resulting solution was stirred for 2 hours at -70°C, the mixture was diluted with 100 mL of water. The resulting solution was extracted with dichloromethane (3x60 mL) and the organic layers combined. The resulting mixture was washed with brine (100 mL), dried over anhydrous sodium sulfate and concentrated to give (Z)-3-((tert-butoxycarbonyl)amino)cyclobutyl methanesulfonate (3) (2.5 g). 'HNMR (400 MHz, CDC13): 5 (ppm): 4.75-4.67 (m, 2H), 3.85-3.82 (m, 1H), 2.98 (s, 3H), 2.93-2.88 (m, 2H), 2.22-2.14 (m, 2H), 1.44 (s, 9H). Synthesis of (E)-tert-butyl (3-cyanocyclobutyl)carbamate trans

[00306] Scheme 2C 3 NaCN, DMF 120°C, 15 h

[00307] (Z)-3-((tert-butoxycarbonyl)amino)cyclobutyl methanesulfonate (3) (1.2 g, 4.5 mmol, 1.0 eq) in DMF (30 mL) was treated with NaCN (665.7 mg, 13.6 mmol, 3.0 eq) and the reaction was heated to 120°C for 15 h, the mixture was diluted with 50 mL of water. The resulting solution was extracted with EA (2x60 mL) and the organic layers combined. The resulting mixture was washed with brine (60 mL), dried over anhydrous sodium sulfate and concentrated to give (E)-tert-butyl (3-cyanocyclobutyl)carbamate (4) (730.0 mg). 'H NMR (400 MHz, CDCI3): 5 (ppm): 4.77 (br s, 1H), 4.41-4.39 (m, 1H), 3.06-3.01 (m, 1H), 2.74-2.41 (m, 2H), 2.27-2.24 (m, 2H), 1.45 (s, 9H). Synthesis of (E)-tert-butyl (3-(N-hydroxycarbamimidoyl)cyclobutyl)carbamate

[00308] Scheme 2D 4 NH2OH, EtOH 80°C,15 h

[00309] To a solution of (E)-tert-butyl (3-cyanocyclobutyl)carbamate (4) (730.0 mg, 3.7 mmol, 1.0 eq) in EtOH (25 mL) was added aqueous hydroxylamine (2.2 g / 2.0 mL, 33.3 mmol, 9.0 eq) under N2. The mixture was heated to 80°C and stirred for 15 h. The reaction mixture was concentrated and purified by Prep-HPLC to give (E)-tert-butyl (3-(N-hydroxycarbamimidoyl)cyclobutyl)carbamate (5) (520.0 mg). LCMS (ESI): m / z 230.2 [M+H]+. Synthesis of (E)-tert-butyl (3-carbamimidoylcyclobutyl)carbamate

[00310] Scheme 2E NH HO^                        Rany Ni, H2 "N                --------------- N'Boc MeOH, 0°C, 8 h trans h 5

[00311] To a solution of (E)-tert-butyl (3-(N-hydroxycarbamimidoyl)cyclobutyl)carbamate (5) (520.0 mg, 2.3 mmol, 1.0 eq) in MeOH (100 mL) was added Raney-Ni (200 mg, 2.3 mmol, 1.0 eq) under N2. The suspension was degassed under vacuum and purged with H2 several times. The mixture was stirred under H2 at 0°C for 8 h. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to give (E)-tert-butyl (3-carbamimidoylcyclobutyl)carbamate (6) (480.0 mg). LCMS (ESI): m / z 214.2 [M+H]+. Synthesis of (E)-tert-butyl ((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3- d]pyrimidin-2-yl)cyclobutyl)carbamate

[00312] Scheme 2F ,Boc O O 7 t-BuOH, TEA 100°C, 15 h

[00313] To a solution of (E)-tert-butyl (3-carbamimidoylcyclobutyl)carbamate (6) (480.0 mg, 2.3 mmol, 1.0 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (7) (431.3 mg, 2.5 mmol, 1.1 eq) in t-BuOH (30 mL) was added TEA (1.3 g, 12.8 mmol, 5.6 eq) in one portion at 15°C under N2. The mixture was heated to 100°C and stirred for 15 h. The reaction was concentrated in vacuo. The residue was purified by silica gel column chromatography (DCM / MeOH = 100%~95%) to give (E)-tert-butyl ((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)cyclobutyl)carbamate (8) (630.1 mg). LCMS (ESI): m / z 338.1 [M+H]+. Synthesis of (E)- 2-((lr,3r)-3-aminocyclobutyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one O trans NH2

[00314] Scheme 2G O                               O

[00315] To a stirred solution of (8) (630 mg, 1.9 mmol, 1.0 eq) in DCM (30.0 mL) was added TFA (3.0 mL, 39.5 mmol, 20.8 eq) at 25°C. The reaction mixture was stirred at 25°C under N2 atmosphere for 5 h. The reaction mixture was concentrated in vacuum to give (E)-2-((lr,3r)-3-aminocyclobutyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (9) (656.5 mg). LCMS (ESI): m / z 238.0 [M+H] +. 'H-NMR (400MHz, DMSO-d6): 5 (ppm): 8.41 (brs, 3H), 3.75-3.71 (m, 1H), 3.47 (s, 2H), 3.43-3.80 (m, 1H), 2.90-2.87 (m, 4H), 2.66-2.53 (m, 4H). Synthesis of (E)-3-nitro-4-(((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3- d]pyrimidin-2-yl)cyclobutyl)amino)benzonitrile

[00316] Scheme 2H O

[00317] To a solution of (9) (443.1 mg, 1.9 mmol, 1.0 eq) and 4-fluoro-3-nitrobenzonitrile (10) (248.3 mg, 1.5 mmol, 0.8 eq) in DMF (30 mL) was added CS2CO3 (1.5 g, 4.6 mmol, 2.4 eq) in one portion at 15°C under N2. The reaction mixture was stirred at 15°C under N2 atmosphere for 15 h, the mixture was diluted with 50 mL of water. The resulting solution was extracted with EA (2x60 mL) and the organic layers combined. The resulting mixture was washed with brine (60 mL), dried over anhydrous sodium sulfate and concentrated to give (E)-3-nitro-4-(((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)cyclobutyl)amino)benzonitrile (11) (500.9 mg). LCMS (ESI): m / z 384.0 [M+H]+. Synthesis of (E)-3-amino-4-(((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)cyclobutyl)amino)benzonitrile

[00318] Scheme 2J CN trans NO2 MeOH, 15°C, 5h 11 NH2 CN trans H2, TEA, Pd / C nh2 CN trans 12

[00319] To a solution of (11) (200.0 mg, 0.52 mmol, 1.0 eq) and TEA (1.0 mL, 7.2 mmol, 13.8 eq) in MeOH (200 mL) was added Pd / C (200 mg) under N2. The suspension was degassed under vacuum and purged with H2 several times. The mixture was stirred under H2 at 15°C for 5 h. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to give (E)-3-amino-4-(((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)cyclobutyl)amino)benzonitrile (12) (180.0 mg). LCMS (ESI): m / z 354.2 [M+H]+.

[00320] Scheme 2K 12                                            Compound 2

[00321] (E)-3-amino-4-(((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3- d]pyrimidin-2-yl)cyclobutyl)amino)benzonitrile (12) (180.0 mg, 0.51 mmol, 1.0 eq) and TEA (3 mL, 21.7 mmol, 42.6 eq) in dichloromethane (DCM) (30 mL) was treated with 1,1’-carbonyldiimidazole (CDI) (918.3 mg, 5.7 mmol, 11.2 eq) and the reaction was heated to 50°C for 15 h, the mixture was diluted with 50 mL of water. The resulting solution was extracted with EA (2x60 mL) and the organic layers combined. The resulting mixture was washed with brine (60 mL), dried over anhydrous sodium sulfate, the organic layers were concentrated and purified by prep-HPLC to give (E)- 2-oxo-l-((lr,3r)-3-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)cyclobutyl)-2,3-dihydro-lH-benzo[d]imidazole-5-carbonitrile (Compound 2) (102.0 mg). LCMS (ESI): m / z 380.0 [M+H]+. 'H-NMR (400MHz, DMSO-d6): 5 (ppm): 11.40 (s, 1H), 8.05 (d, J=8.4 Hz, 1H), 7.58 (dd, J=8.0 Hz, J=1.2 Hz, 1H), 7.40 (d, J=1.2Hz, 1H), 5.02-4.92 (m, 1H), 3.47 (s, 2H), 3.31-3.24 (m, 1H), 3.10-3.02 (m, 2H), 2.93-2.85 (m, 4H), 2.71-2.63 (m, 2H).

[00322] Scheme 2L o Compound 2 Compound 2-P1 0 Compound 2-P2

[00323] (Compound 2) (150 mg) was purified by chiral-HPLC to give (Compound 2-P1) and (Compound 2-P2). LCMS (ESI): m / z 380.0 [M+H] +. 'H-NMR (Compound 2-P1, 400MHz, DMSO-d6): 5 (ppm): 7.49 (s, 2H), 7.38 (s, 1H), 5.10-5.04 (m, 1H), 3.58-3.50 (m, 1H), 3.47 (s, 2H), 3.17-3.09 (m, 2H), 2.93-2.85 (m, 4H), 2.71-2.63 (m, 2H). 'H-NMR (Compound 2-P2, 400MHz, DMSO-d6): 5 (ppm): 12.49 (s, 1H), 11.39 (s, 1H), 8.09 (d, J=8.4 Hz, 1H), 7.58 (dd, J=8.4 Hz, J=1.6 Hz, 1H), 7.40 (d, J=1.6 Hz, 1H), 5.02-4.92 (m, 1H), 3.47 (s, 2H), 3.31-3.24 (m, 1H), 3.10-3.01 (m, 2H), 2.93-2.90 (m, 4H), 2.71-2.63 (m, 2H). Compound 3 Synthesis of 2-(1 -hydr oxy-1,3-dihydrobenzo [c][1,2]oxaborol-5-yl)~ 7,8-dihydro-3H-thiopyrano [4,3-d]pyrimidin-4(5H)-one (Compound 3) Synthesis of methyl 2-bromo-5-cyanobenzoate NC COOMe Br

[00324] Scheme 3A 1 tBuONO, CuBr2~ ACN, R.T., 16h 2 COOMe Br

[00325] A mixture of CuBr2 (3.04 g, 13.6 mmol, 1.2 eq) in CAN (50 mL) was added to tBuONO (1.64 g, 15.9 mmol, 1.4 eq) at 0°C. The mixture was stirred for 5 min. Methyl 2-amino-5-cyanobenzoate (1) (2.0 g, 11.4 mmol, 1 eq) was added in portions. The mixture was stirred for 16 h at room temperature and made acidic (pH = 2) by the addition of 1 M HC1. The mixture was extracted with EtOAc (3 x 80 mL) and the combined organic extract was dried over Na2SO4, filtered, and evaporated to give methyl 2-bromo-5-cyanobenzoate (2) (2.7 g, crude). Synthesis of methyl 2-bromo-5-carbamimidoylbenzoate NH2 HNAp^COOMe Br

[00326] Scheme 3B NC 2 COOMe Br NaOMe, NH4CI, MeOH, 40°C

[00327] A mixture of methyl 2-bromo-5-cyanobenzoate (2) (2.50 g, 10.4 mmol, 1.0 eq), ammonium chloride (0.38 g, 5.4 mmol, 2.5 eq), and sodium methoxide (0.22 g, 5.4 mmol, 2.5 eq) in MeOH (50 mL) was stirred at 40°C for 16 h. The mixture was concentrated to afford methyl 2-bromo-5-carbamimidoylbenzoate (3) (crude). LC-MS (ESI) m / z calculated for C9H9BrN2O2+H+: 257.1; found: 256.9. Synthesis of methyl 2-bromo-5-(4-oxo-4,5,7,8-tetrahydro-3H-thiopyrano[4,3-d]pyrimidin-2- yl) benzoate

[00329] A mixture of methyl 2-bromo-5-carbamimidoylbenzoate (3) (2.5 g, 9.7 mmol, 1.0 eq), methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1.7 g, 9.7 mmol, 1.0 eq), and K2CO3 (4.0 g, 29.2 mmol, 3.0 eq) in MeOH (50 mL) was stirred at 70°C for 16 h. The mixture was cooled and filtered. The filtrate was added into water (300 mL). The solid was filtered and dried to afford methyl 2-bromo-5-(4-oxo-4,5,7,8-tetrahydro-3H-thiopyrano[4,3-d]pyrimidin-2-yl) benzoate (4) (1.5 g, 3.9 mmol). LC-MS (ESI) m / z calculated for CisHi3BrN2O3S+H+: 382.2; found: 383.0. Synthesis of methyl 5-(4-oxo-4,5,7f-tetrahydro-3H-thiopyrano[4,3-d]pyrimidin-2-yl)-2-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl) benzoate

[00331] The mixture of methyl 2-bromo-5-(4-oxo-4,5,7,8-tetrahydro-3H-thiopyrano[4,3-d]pyrimidin-2-yl) benzoate (4) (0.60 g, 1.57 mmol, 1.0 eq), (Bpin)2 (0.80 g, 3.15 mmol, 2.0 eq), Pd(dppf)C12 (115 mg, 0.16 mmol, 0.1 eq), and KO Ac (462 mg, 4.72 mmol, 3.0 eq) in dioxane (15 mL) was stirred at 110°C for 16 h. The mixture was diluted with EtOAc (30 mL), washed with water (15 mL), dried over Na2SO4, and concentrated. The crude product was purified on a silica gel column (PE:EtOAc = 5:1 to 1:1) to afford methyl 5-(4-oxo-4,5,7,8-tetrahydro-3H-thiopyrano[4,3-d]pyrimidin-2-yl)-2-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)benzoate (5) (340 mg, 0.79 mmol). LC-MS (ESI) m / z calculated for C21H25BN2O5S+EE: 429.3; found: 429.1.

[00332] Scheme 3E

[00333] To a solution of methyl 5-(4-oxo-4,5,7,8-tetrahydro-3H-thiopyrano[4,3-d]pyrimidin-2-yl)-2-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)benzoate (5) (0.34 g, 0.79 mmol, 1.0 eq) in THF (15 mL) was added LAH (60 mg, 1.60 mmol, 2.0 eq). The mixture was stirred at 0°C for 1 h. The mixture was added to H2O, filtered, and concentrated under vacuum to afford a crude product which was purified by flash chromatography (H2O:CH3CN = 90:10 to 50:50) to provide 2-(l-hydroxy-l,3-dihydrobenzo[c][l,2]oxaborol-5-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 3). LC-MS (ESI) m / z calculated for C14H13BN2O3S +H+: 301.1; found: 301.0. 'HNMR (400 MHz, MeOD): 5 8.09 (s, 1H), 8.03 (d, J= 7.6 Hz, 1H), 7.84 (d, J= 7.6 Hz, 1H), 3.54 (s, 2H), 5.07 (s, 2H), 2.95-2.85 (m, 4H). Compound 4 and Compound 43 Synthesis of 3-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)~ 1,2,4-oxadiazol-5(4H)-one (Compound 4) O Synthesis of 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzonitrile (Compound 43) O S^>| NH ^^'CN Synthesis of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one O S^>| NH Br

[00334] Scheme 4A 1                                                                            2

[00335] To a solution of methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1) (5.00g, 28.7 mmol, 1.00 eq) and 4-bromobenzamidine hydrochloride (6.76g, 28.7 mmol, 1.00 eq) in EtOH (50 mL) was added K2CO3 (7.93g, 57.4 mmol, 2 eq) at 20°C. The mixture was stirred at 80°C for 16h. LC-MS showed that (1) was consumed completely. The reaction mixture was filtered and the filtrate concentrated under reduced pressure to give a residue that was suspended in water (100 mL), then stirred at 20°C for 4h. The mixture was filtered and the filter cake was dried under reduced pressure to give 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (6.60 g, crude) which was used directly in the next step without further purification. *HNMR (400 MHz, DMSO-t / 6): 3 8.08 - 8.02 (m, 2H), 7.72 - 7.67 (m, 2H), 3.51 (s, 2H), 2.87 (qd, J= 4.4, 8.4 Hz, 4H). LC-MS: 324.3 + bromo isomers (M+1). Synthesis of 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-yl)benzonitrile (Compound 43)

[00336] Scheme 4B

[00337] To a solution of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (4.50g, 13.9 mmol, 1 eq) in NMP (45 mL) were added, portion-wise, Zn(CN)2 (981mg, 8.35 mmol, 530 pL, 0.6 eq) and Pd(PPh3)4 (L61g, 1.39 mmol, 0.1 eq) at 20°C under N2. The mixture was stirred at 100°C for 2h. LC-MS showed that (2) was consumed completely. The resulting mixture was cooled to 20°C and diluted with saturated Na2COs (120 mL) at 20°C. Then the mixture was extracted with EtOAc (40 mL x 3). The combined organic layers were washed with brine, dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue that was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 100 / 1 to 0 / 1). 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzonitrile (Compound 43) (1.80 g, 6.68 mmol) was obtained. 'H NMR (400 MHz, DMSO-de): d 13.07 - 12.77 (m, 1H), 8.25 (br d, J = 7.5 Hz, 2H), 8.00 (d, J = 8.4 Hz, 2H), 3.55 (s, 2H), 2.91 (s, 4H). LC-MS: 270.1 (M+l). Synthesis of (Z)-N ’-hydroxy-4-(4-oxo-3,5,7f-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-

[00339] To a solution of 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzonitrile (Compound 43) (500mg, 1.86 mmol, 1 eq) in pyridine (5 mL) was added NH2OH.HCI (258mg, 3.71 mmol, 2 eq) and K2CO3 (641mg, 4.64 mmol, 2.50 eq) at 20°C. The mixture was stirred at 100°C for 13h. LC-MS showed that (Compound 43) was consumed. After cooling to 20°C, the reaction mixture was filtered and the filter cake dried and concentrated under reduced pressure to give a residue that was suspended in water (5.00 mL) and was stirred at 20°C for 4 h. The mixture was filtered and the filter cake dried and concentrated under reduced pressure to give (Z)-N’-hydroxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzimidamide (4) (333mg, crude), which was used in the next step without further purification. LC-MS: 302.9 (M+l).

[00340] Scheme 4D

[00341] To a solution of (Z)-N’-hydroxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzimidamide (4) (333mg, 1.10 mmol, 1 eq) in pyridine (3.30 mL) was added CDI (268mg, 1.65 mmol, 1.50 eq) at 20°C. The mixture was stirred at 110°C for 3h. LC-MS showed that (4) was consumed completely. After cooling to 20°C, the mixture was filtered and the filter cake dried and concentrated under reduced pressure to give a residue that was stirred in DCM (2 mL) at RT for 6h. The mixture was filtered and the filter cake dried and concentrated under reduced pressure to give 3-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)-l,2,4-oxadiazol-5(4H)-one (Compound 4). 'HNMR (400 MHz, DMSO-t / 6): b 13.26 - 12.46 (m, 1H), 8.23 (d, J= 8.5 Hz, 2H), 7.94 (d, J = 8.5 Hz, 2H), 7.18 (s, 1H), 3.55 (s, 2H), 2.91 (s, 4H). LC-MS: 329.1 (M+l). Compound 5 Synthesis of (E)-(5-(2-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-yl)vinyl)~ 2-(trijluoromethyl)phenyl)boronic acid (Compound 5) Synthesis of 3-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)benzaldehyde 0

[00342] Scheme 5A Pd(dppf)CI2 CH2CI2 (0.1 eq)                   9 P'n2B2 (1 5 eq), KOAc (7.0 eq) DMSO, 25-80°C, 2 hrs 1                                                               2

[00343] To a solution of 3-bromo-4-(trifluoromethyl)benzaldehyde (1) (0.50 g, 1.98 mmol, 1.00 eq) and Pin2B2 (753 mg, 2.96 mmol, 1.50 eq) in DMSO (10.0 mL) was added KO Ac (1.36 g, 13.8 mmol, 7.00 eq) and Pd(dppf)C12.CH2C12 (161 mg, 198 pmol, 0.10 eq) at 25°C under N2. The suspension was degassed in vacuo and purged with N2 several times. The mixture was then warmed to 80°C and stirred at 80°C for 2 h. Thin-layer chromatography (TLC; petroleum ether / ethyl acetate = 20 / 1) showed that 3-bromo-4-(trifluoromethyl)benzaldehyde (1) was consumed completely. The mixture was cooled to 25°C and then diluted with EtOAc (5.00 mL). The mixture was filtered, and the filtrate was washed with water (5.00 mL x 2) and brine (5.00 mL). The organic layer was dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure to give a residue. The residue was purified by Prep-TLC (SiO2, Petroleum ether / Ethyl acetate = 10 / 1). 3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)benzaldehyde (2) (0.20 g, crude) was obtained and used in the next step without further purification. Synthesis of 2-methyl-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one

[00344] Scheme 5B nh2hci HN^ 4 (1.5 eq) K2CO3 (2.5 eq) MeOH 20°C, 12 hrs 3                                     5

[00345] To a solution of acetamidine:hydrochloride (4) (814 mg, 8.61 mmol, 1.50 eq) in MeOH (10.0 mL) was added K2CO3 (1.98 g, 14.4 mmol, 2.50 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3) (1.00 g, 5.74 mmol, 1.00 eq). The mixture was stirred at 20°C for 12 h. TLC (petroleum ether / ethyl acetate = 8 / 1) indicated methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3) was consumed completely. The mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. 2-methyl-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (1.00 g, crude) was obtained and used in the next step without further purification. The obtained compound was checked by 1H NMR. Synthesis of (E)-2-(3-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-4- (trifluoromethyl)styry 1)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00347] To a solution of 2-methyl-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (0.20 g, 666 pmol, 1.21 eq) in AC2O (1.00 mL) was added 3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)benzaldehyde (2) (0.10 g, 549 pmol, 1.00 eq), ZnCh (150 mg, 1.10 mmol, 51.4 uL, 2.00 eq), and AcOH (65.9 mg, 1.10 mmol, 62.8 uL, 2.00 eq) at 25°C under N2. The mixture was stirred at 120°C for 4 h. LC-MS (ET48116-9-P1A) showed 3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)-benzaldehyde (2) was consumed completely and one main peak with the desired m / z was detected. The mixture was cooled to 25°C and then concentrated under reduced pressure to remove most of the AC2O. The resulting residue was dissolved in EtOAc (5.00 mL) and then washed with saturated NaHCOs (2.00 mL x 2). The organic layer was separated and concentrated under reduced pressure to give a residue. The residue was purified by prep-TLC (SiO2, petroleum ether / ethyl acetate = 1 / 1).        (E)-2-(3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)styryl)- 3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (6) (0.25 g, crude) was obtained.

[00348] Scheme 5D

[00349] The mixture of   (E)-2-(3-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-4- (trifluoromethyl)styryl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (6) (0.25 g, 538.44 pmol, 1 eq) in HCI / H2O (4 M, 2.50 mL) was stirred at 80°C for 2 h. LC-MS showed starting material (6) was consumed completely. The reaction mixture was concentrated under reduced pressure to give a residue that was purified by prep-HPLC (column: Phenomenex luna C18 80 * 40 mm * 3 pm; mobile phase: [water (HC1) - ACN]; B%: 18%-48%, 7 min). (E)-(5-(2-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)vinyl)-2-(trifluoromethyl)phenyl)boronic acid (Compound 5) was obtained. LC-MS: 383.0 (M+l). 'HNMR (400MHz, DMSO-d6): 8 = 7.89 (br d, . / =16.4 Hz, 1H), 7.80 - 7.66 (m, 3H), 7.04 (br d, / =16.3 Hz, 1H), 3.51 (br s, 2H), 2.93 - 2.81 (m, 4H). Compound 6 Synthesis of (E)-2-(4-(trifluoromethyl)styryl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 6) O S^ / p'NH ^^cf3 Synthesis of 2-methyl-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one O

[00350] Scheme 6A nh2 HN^ (1.5 eq)          0 I K2CO3 (2.5 eq) _   11 ► s NH I 1^          MeOH 0          20°C, 12 hrs              N 1                                             2

[00351] To a solution of acetamidine / hydrochloride (814 mg, 8.61 mmol, 1.50 eq) inMeOH (10.0 mL) was added K2CO3 (1.98 g, 14.4 mmol, 2.50 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1) (1.00 g, 5.74 mmol, 1.00 eq). The mixture was stirred at 20°C for 12 h. TLC (petroleum ether / ethyl acetate = 8 / 1) indicated starting material (1) was consumed completely. The mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. 2-methyl-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (1.00 g, crude) was obtained and used in the next step without further purification.

[00352] Scheme 6B Compound 6

[00353] To a solution of 2-methyl-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (0.25 g, 1.37 mmol, 1.00 eq) in AC2O (2.50 mL) was added 4-(trifluoromethyl) benzaldehyde (244 mg, 1.40 mmol, 187 pL, 1.02 eq)at20°C. The mixture was stirred at 110°C for 12 h. The mixture was cooled to 20°C and concentrated under reduced pressure to give a residue. The mixture was diluted with H2O (2.00 mL) and extracted with EtOAc (2.00 mL x 3). The organic layer was dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure to give a residue. Part of the residue was purified by prep-HPLC (column: Waters Xbridge BEH C18 100 * 30 mm * 10 pm; mobile phase: [water (lOmM NH4HCO3)-ACN]; B%: 40%-65%, 10 min). (E)-2-(4-(trifluoromethyl)styryl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 6) was obtained. LCMS: 339.1 (M+l). ^NMR (400MHz, DMSO-d6): 8 = 12.62 - 12.51 (m, 1H), 7.91 - 7.73 (m, 5H), 7.04 - 6.92 (m, 1H), 3.52 - 3.45 (m, 2H), 2.89 - 2.79 (m, 4H). Compound 7 Synthesis of 2-(4-(6-bromopyridin-3-yl) phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d] pyrimidin-4-one (Compound 7) O Br Synthesis of 4-(6-bromopyridin-3-yl)benzonitrile Br^hf

[00354] Scheme 7A

[00355] A mixture of (4-cyanophenyl)boronic acid (1) (50.0 g, 340.0 mmol, 1.0 eq), 2-bromo-5-iodopyridine (96.6 g, 340.2 mmol,1.0 eq), and K2CO3 (141.0 g,1.02 mol, 3.0 eq) in dioxane (3.2 L) and H2O (800 mL) was stirred at 60°C for 16 h. The mixture was filtered and concentrated. The crude residue was purified through a silica gel column (PE:EtOAc = 10:1 to 1:1) to afford 4-(6-bromopyridin-3-yl)benzonitrile (2) (63.0 g). LC-MS (ESI) m / z calculated for Ci2H7BrN2+H+: 261.0; found: 261.1 (isotope of 81Br). This reaction is presented as Scheme 7A. Synthesis of 4-(6-bromopyridin-3-yl)-N-hydroxybenzimidamide

[00357] A mixture of 4-(6-bromopyridin-3-yl)benzonitrile (2) (60 g, 231.6 mmol, 1.0 eq), NH2OHHCI (40.2 g, 579 mmol, 2.5 eq), and NaOH (23.16 g, 579 mmol, 2.5 eq) in EtOH (500 mL) was stirred at 80°C for 3 h. The mixture was diluted with EtOH (100 mL) and filtered. The filtrate was concentrated to afford 4-(6-bromopyridin-3-yl)-N-hydroxybenzimidamide (3) (40 g, crude). LC-MS (ESI) m / z calculated for CnHioBrNsO+HL 294.0; found: 294.1 (isotope of 81Br). This reaction is presented as Scheme 7B. Synthesis of 4-(6-bromopyridin-3-yl) benzimidamide 3                                            4

[00359] A mixture of 4-(6-bromopyridin-3-yl)-N-hydroxybenzimidamide (3) (40.0 g, 136.8 mmol, 1.0 eq), NH4C1 (146.48 g, 3.460 mol, 20 eq), and Fe (230.04 g, 2.74 mol, 20 eq) in EtOH (600 mL) was stirred at 80°C for 48 h. The mixture was cooled and filtered. The solvent was removed in vacuo to afford 4-(6-bromopyridin-3-yl) benzimidamide (4) (30. 0 g, crude). LC-MS (ESI) m / z calculated for CnHwBrNs+H4: 276.0; found: 276.1 (isotope of 79Br). This reaction is presented as Scheme 7C.

[00360] Scheme 7D 4                                     Compound 7

[00361] A mixture of 4-(6-bromopyridin-3-yl) benzimidamide (4) (30.0 g, 108.6 mmol, 1.0 eq), methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (37.8 g, 217.2 mmol, 2 eq), and K2CO3 (45.0 g, 325.8 mmol, 3.0 eq) in MeOH (500 mL) was stirred at 70°C for 4 h. The mixture was concentrated under reduced pressure. The residue was washed with H2O (200 mL) and filtered. The solid was triturated in MeOH (150 mL), EtOAc (150 mL), and acetone (100 mL), respectively, to remove most of the impurities. The mixture was filtered off. The crude solid was triturated in aqueous NaOH (1% wt, 20 mL) and filtered. The solid was washed with water (50 mL) and acetone (100 mL) and dried under vacuum to afford 2-(4-(6-bromopyri din-3-yl) phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one (Compound 7). LC-MS (ESI) m / z calculated for CisHmBfNsOS+HL 402.0; found: 401.9 (isotope of 81Br). 'H NMR (400 MHz, DMSO): 5 12.82 (brs, 1H), 8.81 (d, J = 2.4 Hz, 1H), 8.22 (d, J = 8.3 Hz, 2H), 8.14 (dd, J = 8.4, 2.4 Hz, 1H), 7.91 (d, J = 8.3 Hz, 2H), 7.77 (d, J = 8.4 Hz, 1H), 3.54 (s, 2H), 2.91 (brs, 4H). This reaction is presented as Scheme 7D. Compound 8 Synthesis of 2-(4-(2-(2-hydroxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 8) O Synthesis of methyl 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-ylfbenzoate O

[00362] Scheme 8A NHHCI O 1                                                                  2

[00363] To the solution of methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1) (2 g, 11.48 mmol, 1 eq) in MeOH (20 mL) was added methyl 4-carbamimidoylbenzoate hydrochloride (la) (3.20 g, 14.91 mmol, 1.30 eq) and K2CO3 (4.00 g, 28.94 mmol, 2.52 eq) at 25°C. The mixture was stirred at 25°C for 12 h. The mixture was filtered, and the filter cake was concentrated under reduced pressure to give a residue. The residue was suspended in H2O (40 mL) and stirred at 25°C for 12 h. The mixture was filtered, and the filter cake was dried in vacuo to give methyl 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzoate (2) (1.5 g, crude). The crude product was used in the next step directly without further purification. This reaction is presented as Scheme 8A. Synthesis of 2-(4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one

[00364] Scheme 8B 2                                                       3

[00365] To a solution of methyl 4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzoate (2) (0.3 g, 992.24 pmol, 1 eq) in THF (3 mL), MeMgBr (3 M, 992.24 pL, 3 eq) was added dropwise at 0°C under N2. The mixture was stirred at 25°C for 3 h. The reaction mixture was quenched by the addition of NH4CI (5 mL), and the aqueous phase was extracted with 10 mL of DCM (5 mL x 2). The combined organic layers were concentrated under reduced pressure to give a residue. 2-(4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (2 g, crude) was obtained, which was used in the next step directly without further purification. 'H NMR: ET47629-1-P1A (400 MHz, CDCI3): 3 12.54 - 12.27 (m, 1H), 8.07 (d, J = 8.5 Hz, 2H), 7.56 (d, J = 8.5 Hz, 2H), 3.62 (br s, 2H), 3.03 - 2.94 (m, 2H), 2.91 - 2.82 (m, 2H), 1.55 (s, 6H). This reaction is presented as Scheme 8B.

[00366] Scheme 8C O                                   O 3                                           Compound 8

[00367] To a solution of 2-(4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (0.12 g, 396.84 pmol, 1 eq) in ethylene glycol (5 mL) was added TosOH (82.00 mg, 476.21 pmol, 1.2 eq) at 20°C. The mixture was stirred at 20°C for 1 h, then warmed to 80°C and stirred at 80°C for 12 h. The mixture was purified directly by prep-HPLC (neutral condition, column: Waters Xbridge BEH C18 100 * 30 mm * 10 pm; mobile phase: [water (NH4HCO3)-ACN]; B%: 10%-50%, 10 min). 2-(4-(2-(2-hydroxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 8) was obtained. LC-MS: 347.2 (M +1). 'HNMR: ET47430-4-P1A1 (400 MHz, CDC13): b 8.14 (d, J = 8.2 Hz, 2H), 7.60 (d, J = 8.3 Hz, 2H), 3.80 - 3.69 (m, 4H), 3.34 (t, J = 4.6 Hz, 2H), 3.14-3.06 (m, 2H), 3.01 - 2.93 (m, 2H), 1.62 (s, 6H). This reaction is presented as Scheme 8C. Compound 9 Synthesis of2-(4'-bromo-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 9) O NH Br Synthesis of 4'-bromo-[l, 1 '-biphenyl]-4-carboximidamide

[00369] To a mixture of 4'-bromo-[l,T-biphenyl]-4-carbonitrile (1) (4 g, 15.5 mmol, 1 eq) in THF (40 mL) was added NaHMDS (1 M, 18.60 mL, 1.2 eq) dropwise at 25°C under N2. The mixture was stirred at 25°C for 1 h, and a liquid was obtained. The reaction mixture was quenched by the addition of water (30 mL) at 25°C. The pH of the mixture was adjusted to 2~3 by addition of 1 M HC1. The aqueous layer was separated and evaporated to about 15% of its original volume, resulting in crystallization of the desired HCl-salts. The mixture was filtered, and the filter cake was dried in vacuo. 4'-bromo-[l,T-biphenyl]-4-carboximidamide (2) (4.53 g, 14.5 mmol, HC1) was obtained. The crude product was used in the next step without further purification. ^NMR: (400 MHz, DMSO-d6): b 9.44 (s, 2H), 9.19 (s, 2H), 7.94 (s, 4H), 7.81-7.65 (m, 4H).

[00370] Scheme 9B 2                                              Compound 9

[00371] To a solution of 4'-bromo-[l,T-biphenyl]-4-carboximidamide (2) (0.066 g, 378.8 pmol, 1.1 eq) in MeOH (1.8 mL) was added methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3) (107.3 mg, 344.4 pmol, 1 eq, HC1) and K2CO3 (95.2 mg, 688.8 pmol, 2 eq) at 25°C. The mixture was stirred at 25°C for 16 h. The reaction mixture was filtered, and the filter cake was dried in vacuo to give a residue. The crude product was purified by precipitation from DMSO (3 mL). 2-(4'-bromo-[l,r-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 9) was obtained. LC-MS: 399.19 (M+l). 'HNMR: ET48394-6-P1A (400 MHz, DMSO-d6): 3 12.86 - 12.62 (m, 1H), 8.21 (d, J = 8.3 Hz, 2H), 7.86 - 7.81 (m, 2H), 7.75 - 7.68 (m, 4H), 3.55 (s, 2H), 2.91 (s, 4H). Compound 10 Synthesis of (4 '-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[1,1 -biphenyl]-4-yl)boronic acid (Compound 10) Synthesis of 2-(4'-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-[1,1 '-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00372] Scheme 10A o Compound 9                                                5

[00373] To a mixture of 2-(4'-bromo-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 9) (0.31 g, 776.4 pmol, 1 eq) and B2Pin2 (394.3 mg, 1.55 mmol, 2 eq) in dioxane (6 mL) was added KOAc (152.4 mg, 1.55 mmol, 2 eq) and Pd(dppf)C12.CH2C12 (63.4 mg, 77.6 pmol, 0.1 eq) in portions at 25°C under N2. The mixture was degassed under vacuum and purged three times with N2. Then the reaction mixture was heated to 100 °C and stirred for 16 h. A suspension liquid was obtained. The crude product was triturated with H2O (10 mL) at 25°C for 2 h. The mixture was filtered, and the filter cake was washed with DCM (5 mL) and MeOH (5 mL). The filter cake was dried under reduced pressure to give 2-(4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (0.18 g) which was used in the next step without further purification.

[00374] Scheme 10B Compound 10

[00375] To a round-bottom flask was added 2-(4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (0.18 g, 403.2 pmol, 1 eq) and HC1 (4 M, 4 mL, 39.7 eq) at 25°C. The reaction mixture was stirred at 80°C for 4 h. The mixture was filtered, and the filter cake was washed with DCM (5 mL) and MeOH (5 mL). The filter cake was dried in vacuo. (4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid (Compound 10) was obtained. LC-MS: 365.1 (M+l). 'HNMR: ET48394-20 (400 MHz, DMSO-d6): 3 8.20 (d, J= 8.5 Hz, 2H), 7.89 (dd, J = 8.3, 19.4 Hz, 4H), 7.74 (d, J = 8.2 Hz, 2H), 3.55 (s, 2H), 2.92 (s, 4H). Compound 11 Synthesis of 2-(4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one (Compound 11)

[00376] Scheme HA OH HO^Y^N ^N^CI 2 (1.1 eq) NC Pd(dppf)CI2 (0.1 eq), K2CO3 (2 eq) -----------------► Dioxane, H2O (2 vol) 25-100°C, 16 hrs 1

[00377] To a mixture of (2-chloropyrimidin-5-yl)boronic acid (2) (9.57 g, 60.43 mmol, 1.10 eq) and 4-bromobenzonitrile (1) (10.0 g, 54.9 mmol, 1.00 eq) in dioxane (200 mL) and H2O (20.0 mL) was added K2CO3 (15.2 g, 110 mmol, 2.00 eq) and Pd(dppf)C12.CH2C12 (4.49 g, 5.49 mmol, 0.10 eq) in one portion at 25°C under N2. The mixture was degassed with N2 and then stirred at 100°C for 16 h. A liquid was obtained. The reaction mixture was diluted with water (200 mL) and extracted with EtOAc 600 mL (200 mL x 3). The combined organic layers were concentrated under reduced pressure to give a residue that was purified by flash silica gel chromatography (ISCO®; 200 g SepaFlash® Silica Flash Column, eluent of 0 ~ 20% ethyl acetate / petroleum ether gradient @ 100 mL / min, Rf = 0.25). 4-(2-chloropyrimidin-5-yl)benzonitrile (3) (1.1 g) was obtained and used as a crude in the next step. Synthesis of 4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)benzonitrile NC

[00378] Scheme 1 IB

[00379] To a mixture of 4-(2-chloropyrimidin-5-yl)benzonitrile (3) (0.70 g, 3.25 mmol, 1.00 eq) in THF (4.00 mL) and ethylene glycol (4.00 mL) was added K2CO3 (897 mg, 6.49 mmol, 2.00 eq) in one portion at 25°C under N2. The mixture was stirred at 70°C for 16 h. A suspension liquid was obtained. The reaction mixture was diluted with water (20 mL) and extracted with EtOAc 30 mL (10 mL x 3). The combined organic layers were concentrated under reduced pressure to give a residue that was purified by flash silica gel chromatography (ISCO®; 40 g SepaFlash® Silica Flash Column, eluent of 0 ~ 50% ethyl acetate / petroleum ether gradient @ 40 mL / min, Rf = 0.27). 4-(2-(2-hydroxyethoxy )pyrimidin-5-yl)benzonitrile (4) (540 mg, 2.24 mmol) was obtained and used crude in the next step. Synthesis of (Z)-N'-hydroxy-4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)benzimidamide

[00381] To a mixture of 4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)benzonitrile (4) (0.54 g, 2.24 mmol, 1.00 eq) inMeOH (5.00 mL) was added NH2OH.HCI (171 mg, 2.46 mmol, 1.10 eq) and NaHCOs (207 mg, 2.46 mmol, 95.8 pL, 1.10 eq) in one portion at 25°C under N2. The mixture was stirred at 65°C for 16 h. A liquid was obtained. The reaction mixture was filtered and concentrated under reduced pressure to give a (Z)-N'-hydroxy-4-(2-(2- hydroxyethoxy)pyrimidin-5-yl)benzimidamide (5) (500 mg, crude) and used in the next step without further purification. Synthesis of 4-(2-(2-hydroxyethoxy)pyrimidin-5-yl) benzimidamide NH

[00382] Scheme 1 ID Ac2O (4 eq) H2 (15 psi), Pd / C(10%) MeOH, AcOH (5 vol) 25°C, 16.5 hrs NH 6 5

[00383] To a mixture of   (Z)-N'-hydroxy-4-(2-(2-hydroxy ethoxy)pyrimidin-5- yl)benzimidamide (5) (0.50 g, 1.82 mmol, 1.00 eq) in AcOH (2.62 g, 43.7 mmol, 2.50 mL) was added AC2O (744 mg, 7.29 mmol, 683 pL, 4.00 eq) in one portion at 25°C under N2. The mixture was stirred at 25°C for 30 min. MeOH (20 mL) and Pd / C (0.2 g, 218.76 pmol) were then added. The resulting mixture was degassed three times with H2 and stirred under H2 (15 psi) at 25°C for 16 h. The reaction mixture was filtered and concentrated under reduced pressure to give 4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)benzimidamide (6) (500 mg, crude) and used in the next step without further purification.

[00384] Scheme HE Compound 11

[00385] To a mixture of 4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)benzimidamide (6) (500 mg, 1.94 mmol, 1.00 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (7) (438 mg, 2.52 mmol, 1.30 eq) in MeOH (4.00 mL) was added K2CO3 (535 mg, 3.87 mmol, 2.00 eq) in one portion at 25°C under N2. The mixture was stirred at 25°C for 16 h. The reaction mixture was filtered and concentrated under reduced pressure to give a residue that was purified by prep-HPLC (column: Waters Xbridge Prep OBD Cl8 150 * 40 mm * 10 pm; mobile phase: [water (NH4HCO3) - ACN]; B%: 10% - 40%, 8 min). 2-(4-(2-(2-hydroxyethoxy)pyrimidin-5-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 11) was obtained. 'H NMR (400 MHz, DMSO-d6) 5 = 13.15 - 12.49 (m, 1H), 9.03 (s, 2H), 8.21 (br d, J = 8.2 Hz, 2H), 7.91 (d, J = 8.6 Hz, 2H), 4.93 (t, J = 5.5 Hz, 1H), 4.42 - 4.36 (m, 2H), 3.76 (q, J = 5.4 Hz, 2H), 3.54 (s, 2H), 2.95 - 2.86 (m, 4H). LCMS: 383 (M+1). Compound 12 Synthesis of 2-(4'-(2-(2-hydroxyethoxy)propan-2-yl)-[ 1, l'-biphenyl] -4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 12) O NH Synthesis of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one 0

[00386] Scheme 12A 1                                                               2

[00387] To a mixture of methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1) (8.00 g, 45.9 mmol, 1.00 eq) and 4-bromobenzimidamide (3) (11.9 g, 50.5 mmol, 1.10 eq, HC1) in EtOH (100 mL) was added K2CO3 (12.7 g, 91.8 mmol, 2.00 eq) in one portion at 25°C under N2. The mixture was stirred at 80°C for 16 h. The reaction mixture was filtered and concentrated under reduced pressure to give 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (15.6 g, crude) that was used in the next step without further purification. 'H NMR: (400 MHz, DMSO-d6): 5 8.19 - 8.13 (m, 2H), 7.53 - 7.46 (m, 2H), 3.42 (s, 2H), 2.81 - 2.75 (m, 2H), 2.74 - 2.67 (m, 2H). Synthesis of 2-(4'-(2-hydroxypropan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00388] Scheme 12B OH 2                                                                      5

[00389] To a mixture of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (4.00 g, 12.4 mmol, 1.00 eq) and (4-(2-hydroxypropan-2-yl)phenyl)boronic acid (4) (2.67 g, 14.9 mmol, 1.20 eq) in dioxane (40.0 mL) and H2O (5.00 mL) was added K2CO3 (3.42 g, 24.8 mmol, 2.00 eq) and Pd(dppf)C12.CH2C12 (1.01 g, 1.24 mmol, 0.10 eq) in one portion at 25°C under N2. The mixture was degassed under vacuum, purged three times with N2, and then heated to 100°C and stirred for 16 h. The reaction mixture was diluted with EtOAc (20.0 mL) and then filtered. The filter cake was washed with water (10 mL) and MeOH (10 mL) to give 2-(4'-(2-hydroxypropan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (2.10 g, 5.55 mmol, crude) and used in the next step without further purification. 'H NMR: (400 MHz, DMSO-d6): 5 = 13.13 - 12.31 (m, 1H), 8.51 - 7.75 (m, 4H), 7.75 - 7.15 (m, 4H), 5.07 (br d, J = 3.2 Hz, 1H), 3.54 (br s, 2H), 2.90 (br s, 4H), 1.78 - 0.88 (m, 6H).

[00390] Scheme 12C Compound 12

[00391] To a mixture of 2-(4'-(2-hydroxypropan-2-yl)-[l, l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (1.50 g, 3.96 mmol, 1.00 eq) in DMF (10.0 mL) was added TosOH (819 mg, 4.76 mmol, 1.20 eq) and ethylene glycol (5.55 g, 89.4 mmol, 5.00 mL, 22.6 eq) in one portion at 25°C under N2. The mixture was stirred at 85°C for 16 h. The mixture was cooled to RT and filtered. The filter cake was dissolved in DMSO and then purified directly by prep-HPLC (column: Phenomenex Luna 80 * 30 mm * 3 pm; mobile phase: [water (HC1) - MEOH]; B%: 40% - 65%, 8 min) to give 2-(4'-(2-(2-hydroxyethoxy)propan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3- d]pyrimidin-4-one (Compound 12). LCMS: 423.2 (M+l). 'H NMR: (400 MHz, CHLOROFORM-d): 5 11.47 - 10.94 (m, 1H), 8.14 (d, J = 8.4 Hz, 2H), 7.76 (d, J = 8.5 Hz, 2H), 7.67 - 7.61 (m, 2H), 7.54 (d, J = 8.4 Hz, 2H), 3.80 - 3.69 (m, 4H), 3.41 - 3.31 (m, 2H), 3.10-3.03 (m, 2H), 2.99-2.91 (m, 2H), 1.66- 1.62 (m, 6H). Compound 13 Synthesis of 2-(4-(trifluoromethyl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimi-din-4-one-8,8-d2 (Compound 13) O S'^rf'NH D D II .1 ^^CF3 Synthesis of methyl-d3 4-oxotetrahydro-2H-thiopyran-3-carboxylate-3,5,5-d3

[00392] Scheme 13A NaH (0.01 eq) MeOD, 65°C, 2 h

[00393] A mixture of methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (1) (0.9 g, 5.16 mmol, 1.0 eq) and NaH (2.1 mg, 0.051 mmol, 0.01 eq) in MeOD (13.5 mL) was stirred at 65°C for 2 h. Methyl-d3 4-oxotetrahydro-2H-thiopyran-3-carboxylate-3,5,5-d3 (2) was obtained as a crude product in MeOD solution. 'H NMR (400 MHz, MeOD): 5 3.23-3.07 (m, 1H), 2.95 (s, 1H), 2.76 (s, 1H), 2.73 (s, 2H).

[00394] Scheme 13B O S               K2CO3, MeOD, 2                    65°C,1 h 5.07 mmol. 1.11 ea\ and

[00395] A mixture of (2)   (0.90 g, 4-(trifluoromethyl)benzimidamide (0.861 g, 4.56 mmol, 1.0 eq) in MeOD (12 mL) was stirred at 65°C for 1 h. The mixture was concentrated to a half volume, then added to H2O (50 mL). The precipitate was filtered and washed with H2O (30 mL) and MeOH / H2O (20 mL, 1:1, v / v), and dried in vacuo to afford 2-(4-(trifluoromethyl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimi-din-4-one-8,8-d2 (Compound 13). LC-MS (ESI) m / z calculated for Ci4H9D2F3N2OS+H+: 315.1; found: 315.1. XH NMR (400 MHz, DMSO-d6): 8 8.29 (d, J = 8 Hz, 2H), 7.88 (d, J= 8 Hz, 2H), 3.54 (s, 2H), 2.89 (s, 2H). 19F NMR (375 MHz, DMSO-d6): 6 61.33 (s, 3F). Compound 14 Synthesis of (2,6-difluoro-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2- yl)phenyl)boronic acid (Compound 14) Synthesis of 3,5-difluorobenzimidamide

[00397] To a solution of 3,5-difluorobenzonitrile (1) (5.0 g, 36.0 mmol, 1.0 eq) in MeOH (50 mL) was added MeONa (3.9 g, 71.9 mmol, 2.0 eq); the mixture was stirred at 20°C for 4 h. To the mixture was added NH4CI (3.9 g, 71.9 mmol, 2.0 eq), and then the mixture was stirred at 40°C for 16 h. It was cooled to RT, filtered, and then the filtrate was concentrated to give 3,5-difluorobenzimidamide (2) (7 g, crude). LC-MS 157.4 [M+H]+. Synthesis of 2-(3,5-difluorophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one 0 S^>| NH F

[00398] Scheme 14B

[00399] To a mixture of 3,5-difluorobenzimidamide (2) (7 g, 44.9 mmol, 1.0 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3) (6.2 g, 35.9 mmol, 0.8 eq) in MeOH (50 mL) was added K2CO3 (12.4 g, 89.7 mmol, 2.0 eq) under N2. The mixture was stirred at 75 °C for 2 h. The reaction mixture was concentrated to give 2-(3,5-difluorophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4), which was used directly for the next step. LC-MS 281.1 [M+H]+. Synthesis of 2-(3,5-difluorophenyl)-4-(2-methoxyethoxy)methoxy)-7,8-dihydro-5H- thiopyrano[4,3-d]pyrimidine ^nAQf F

[00400] Scheme 14C O I J JL ___ F MEMCI, K2CO3, DMA * U J            rt,3h MEM O ^nAQf

[00401] To a mixture of 2-(3,5-difluorophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (crude, 1.0 eq) in dimethylacetamide (DMA) (50 mL) was added l-(chloromethoxy)-2-methoxyethane (11.2 g, 89.7 mmol, 2.0 eq). The reaction mixture was stirred at RT for 3 h and then poured into H2O (300 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was washed with brine (300 mL), dried over Na2SO4, filtered, and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-30% EtOAc / hexane to afford 2-(3,5-difluorophenyl)-4-((2-methoxyethoxy)methoxy)-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine (5) (6 g). LC-MS: 369.2 [M+H]+. Synthesis of (2,6-dijluoro-4-(4-((2-methoxyethoxy)methoxy)-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl) boronic acid MEM.

[00403] To a mixture of 2-(3,5-difluorophenyl)-4-((2-methoxyethoxy)methoxy)-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine (5) (400 mg, 1.4 mmol, 1.0 eq) in dry THF (10 mL) cooled at -78°C was added lithium diisopropylamide (LDA) (2 M, 1.1 mL, 1.5 eq) dropwise; the mixture was stirred at -78°C for 1 h. Trimethyl borate was added (191 mg, 1.9 mmol, 1.3 eq) and then the mixture was stirred at -78°C for 3 h. The mixture was quenched with NH4CI solution (30 mL) and extracted with EtOAc (2x20 mL). The combined organic layer was washed with brine (50 mL), dried over Na2SO4, filtered, and concentrated. The residue was purified by reverse-phase chromatography (0-50% acetonitrile / 0.05% formic acid) to afford (2,6-difluoro-4-(4-((2-methoxyethoxy)methoxy)-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (6) (200 mg). LC-MS: 413.4 [M+H]+.

[00404] Scheme 14E

[00405] A mixture of (2,6-difluoro-4-(4-((2-methoxy ethoxy )methoxy)-7,8-dihydro-5H- thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (6) (200 mg, 0.48 mmol, 1.0 eq) in formaldehyde (FA) (3 mL) was stirred at room temperature (RT) for 2 h. It was then concentrated and purified by Prep-HPLC to afford (2,6-difluoro-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 14). LC-MS 325.1 [M+H]+. 'H NMR (400MHz, DMSO4): 5 12.85 (s, 1H), 8.86 (s, 2H), 7.72 (d, J = 7.2 Hz, 2H), 3.54 (s, 2H), 2.90 (s, 4H). Compound 15 Synthesis of 2-(3,5-difluoro-4-(2-(2-hydroxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro- 4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 15) Synthesis of methyl 4-cyano-2,6-difluorobenzoate

[00406] Scheme 15A TMSCH2N2 (1.5 eq) THF / MeOH (10:1) 25°C, 13 hrs

[00407] To a solution of 4-cyano-2,6-difluorobenzoic acid (1) (5.00 g, 27.31 mmol, 1.0 eq) in THF, (50 mL) / MeOH (50 mL) TMSCHN2 (2 M, 20.48 mL, 1.5 eq) was added dropwise at 25°C. The mixture was stirred at 25°C for 13 h. TLC indicated (petroleum ether / ethyl acetate = 5 / 1, Rf(Cpd.l) = 0.33) (1) was consumed completely. The reaction mixture was concentrated under reduced pressure to give a residue. Methyl 4-cyano-2,6-difluorobenzoate (2) (4.1 g, crude) was obtained. Synthesis of methyl 4-carbamimidoyl-2,6-dijluorobenzoate

[00409] To a solution of methyl 4-cyano-2,6-difluorobenzoate (2) (2.00 g, 10.15 mmol, 1.0 eq) in THF (20 mL) was added LiHMDS (1 M, 15.22 mL, 1.5 eq) at 0°C. The mixture was stirred at 20°C for 16 h. LC-MS (ET68120-10-P1A1) showed that (2) was consumed completely, and the desired mass was detected. The reaction mixture was quenched by addition of HCl / dioxane (4 mol / L, 8 mL) at 0°C, and then concentrated under reduced pressure to give a residue. The residue was triturated with MeOH (40 mL) and the mixture was stirred at 20°C for 1 h. Then the mixture was filtered, and the filter cake was dried under reduced pressure. Methyl 4-carbamimidoyl-2,6-difluorobenzoate (3) (2.6 g, crude, HC1) was used directly in the next step without further purification. Synthesis of methyl 2,6-difluoro-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin- 2-yl)benzoate F O

[00410] Scheme 15C F O 3                                                         4

[00411] To a solution of methyl 4-carbamimidoyl-2,6-difluorobenzoate (3) (470 mg, 1.88 mmol, 1.0 eq, HC1) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3a) (327 mg, 1.88 mmol, 1.0 eq) inMeOH (4.7 mL) was added K2CO3 (778 mg, 5.63 mmol, 3.0 eq) at 25°C. The mixture was stirred at 25°C for 16 h. LC-MS showed that (3) was consumed completely, and one peak with the desired mass was detected. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (neutral condition; column: Waters Xbridge Prep OBD Cl8 150 x 40 mm x 10 pm; mobile phase: [water (NH4HCO3) - ACN]; B%: 10% - 40%, 8 mins). Methyl 2,6-difluoro-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzoate (4) (180 mg) was obtained. Synthesis of 2-(3,5-dijluoro-4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00413] To a solution of methyl 2,6-difluoro-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)benzoate (4) (300 mg, 887 pmol, 1.0 eq) in THF (5.0 mL) was added MeMgBr (3 M, 1.03 mL, 3.5 eq) at 0°C. The mixture was stirred at 25°C for 1 h. TLC (petroleum ether / ethyl acetate =1 / 1, Rf (Cpd.4) = 0.60) indicated that (4) was consumed completely, and two new spots formed. LC-MS (ET65158-9-P1A1) showed that (4) was consumed completely and one main peak with the desired MS was detected. The reaction mixture was quenched by the addition of NH4CI (15 ml) at 0°C, and then extracted with 45 mL of EtOAc (15 mL x 3). The combined organic layers were dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue. The residue was purified by Prep-TLC (petroleum ether / ethyl acetate = 1 / 1). 2-(3,5-difluoro-4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (150 mg, crude) was obtained and used immediately in the next step. LC-MS (ET65158-9-P1A1, product: Rt = 0.575 mins).

[00414] Scheme 15E O                                     O 5                                             Compound 15

[00415] To a solution of 2-(3,5-difluoro-4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (20.0 mg, 59.11 pmol, 1.0 eq) in ethylene glycol (1.59 g, 25.62 mmol, 1.43 mL, 433.41 eq) was added TosOH (12.7 mg, 73.88 pmol, 1.25 eq) at 25°C. The mixture was stirred at 80°C for 12 h. TLC (petroleum ether / ethyl acetate = 0 / 1, Rf(Cpd.5) = 0.53) indicated that (5) was consumed completely, and two new spots formed. After cooling to 25°C, the mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-TLC (SiO2, petroleum ether / ethyl acetate = 0 / 1) to provide 2-(3,5-difluoro-4-(2-(2-hydroxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 15) was obtained. LC-MS: 383.1 (M+l). 'HNMR (400 MHz, DMSO-t / e): 3 = 1.18 - 7.70 (m, 2H), 4.49 - 4.45 (m, 1H) 3.47 - 3.43 (m, 4H), 3.21 - 3.17 (m, 2H), 2.85-2.75 (m, 4H), 1.637 (s, 6H). Compound 16 Synthesis of 2-(3,5-dijluoro-4-(2-(2-methoxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro- 4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 16) Compound 16

[00417] Synthesis of 2-(3,5-difluoro-4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) is shown in Schemes 15A-15D.

[00418] To a solution of 2-(3,5-difluoro-4-(2-hydroxypropan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (50.0 mg, 147 pmol, 1.0 eq) in 2-methoxyethanol (0.5 mL) was added TosOH (31.6 mg, 183 pmol, 1.24 eq) at 25°C and then heated to 85°C and stirred for 13 h. LC-MS showed -17% of (5) remained. Several new peaks were shown on LC-MS, and the desired compound was detected. After cooling to 25°C, the reaction mixture was purified by prep-HPLC (neutral condition; column: Waters Xbridge BEH C18 100 x 30mm x lOum; mobile phase: [water (NH4HCO3) - can]; B%: 25%-55%, 8 mins) to provide 2-(3,5-difluoro-4-(2-(2-methoxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 16). LC-MS: 397.1 (M+1). 1HNMR(400 MHz, DMSO-^): 3 = 7.81-7.75 (m, 2H), 3.549 (s, 2H) 3.53-3.38 (m, 2H), 3.35-3.25 (m, 2H), 3.22 (s, 3H), 2.906 (m, 4H), 1.653 (s, 6H). Compound 17 Synthesis of 2-(4-(2-(2-methoxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one (Compound 17) 0

[00419] Scheme 17A

[00420] To solution of 2-(4-bromophenyl)propan-2-ol (1) (2.6 g, 12.2 mmol, 1.0 eq) in 2-methoxyethan-l-ol (20 mL) was added TSOH H2O (2.3 g, 12.2 mmol, 1 eq). The mixture was stirred at room temperature (RT) for 2 days, then quenched with water (100 mL) and extracted with EtOAc (3 x 60 mL). The organic layers were concentrated and the residue was purified by column chromatography on silica gel eluted with 0-30% EtOAc / hexane to afford 1-bromo-4-(2-(2-methoxyethoxy)propan-2-yl)benzene (2) (2.7 g). 'H NMR (400MHz, DMSO-t / e): 5 7.54-7.51 (m, 2H), 7.38-7.35 (m, 2H), 3.42-3.40 (m, 2H), 3.24-3.22 (m, 5H), 1.44 (s, 6H). Synthesis of 4-(2-(2-methoxyethoxy)propan-2-yl)benzonitrile

[00421] Scheme 17B NC ([   1                   Zn(CN)2, Pd(PPh3)4 |\             NMP, 140°C, 2h 2                                                 3

[00422] To a solution of l-bromo-4-(2-(2-methoxyethoxy )propan-2-yl)benzene (2) (1.5 g, 3.7 mmol, 1 eq) inNMP (10 mL) was added Zn(CN)2 (260 mg, 2.2 mmol, 0.6 eq) andPd(PPh3)4 (430 mg, 0.37 mmol, 0.1 eq). The resulting solution was stirred for 2 h at 140°C under Ar. The resulting mixture was cooled to RT, diluted with water (50 mL), and extracted with EtOAc (3x60 mL). The organic layers were combined, washed with brine (100 mL), dried over anhydrous sodium sulfate, and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-40% EtOAc / hexane to give 4-(2-(2-methoxyethoxy)propan-2-yl)benzonitrile (3) (600 mg). 'H NMR (400MHz, DMSO-^): 5 7.81 (d, J= 8.4 Hz, 2H), 7.62 (d, J = 8.4 Hz, 2H), 3.44 (t, J = 5.2 Hz, 2H), 3.28-3.25 (m, 5H), 1.47 (s, 6H). Synthesis of 4-(2-(2-methoxyethoxy)propan-2-yl)benzimidamide

[00424] To a solution of 4-(2-(2-methoxyethoxy)propan-2-yl)benzonitrile (3) (0.6 g, 2.7 mmol, 1.0 eq) in MeOH (5 mL) was added MeONa (295 mg, 5.5 mmol, 2.0 eq); the mixture was stirred at RT for 4 h. NH4CI (287 mg, 5.5 mmol, 2.0 eq) was added and then the mixture was stirred at 40°C for 16 h. The mixture was cooled to RT, filtered, and then the filtrate was concentrated to give 4-(2-(2-methoxyethoxy)propan-2-yl)benzimidamide (4) (285 mg, crude). LC-MS: 237.2 [M+H]+.

[00425] Scheme 17D

[00426] To a solution of 4-(2-(2-methoxyethoxy)propan-2-yl)benzimidamide (4) (150 mg, 0.7 mmol, 1.0 eq) in MeOH (5 mL) was added K2CO3 (180 mg, 1.3 mmol, 2.0 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (80 mg, 0.5 mmol, 0.7 eq) under N2. The mixture was stirred at 70°C for 2 h. It was then concentrated and purified by Prep-HPLC to give 2-(4-(2-(2-methoxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 17). LC-MS: 361.1 [M+H]+. 'HNMR (400MHz, DMSO4): 5 12.70 (s, 1H), 8.06 (d, J= 8.6 Hz, 2H), 7.54 (d, J = 8.6 Hz, 2H), 3.52 (s, 2H), 3.46-3.41 (m, 2H), 3.25 (d, J= 5.0 Hz, 5H), 2.88 (dd, J= 7.6, 4.0 Hz, 4H), 1.48 (s, 6H). Compound 18 Synthesis of methyl (2-((2-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-yl)phenyl)propan-2-yl)oxy)ethyl)carbamate (Compound 18) O Synthesis of l-bromo-4-(2-(2-nitroethoxy)propan-2-yl)benzene

[00428] To a solution of 2-(4-bromophenyl)propan-2-ol (1) (2.6 g, 12.2 mmol, 1.0 eq) in DCM (20 mL) was added TFA (2.3 g, 12.2 mmol, 1.0 eq) and 2-nitroethan-l-ol (2) (10 mL). The mixture was stirred at RT for 3 days. The reaction was quenched with water (100 mL) and extracted with EtOAc (3 x 60 mL). The organic layers were concentrated, and the residue was purified by column chromatography on silica gel eluted with 0-30% EtOAc / hexane to afford l-bromo-4-(2-(2-nitroethoxy)propan-2-yl)benzene (3) (2.7 g). 'HNMR (400 MHz, CDC13): 5 7.80 (d, J = 8.8 Hz, 2H), 7.31 (d, J= 8.4 Hz, 2H), 4.55 (t, J = 4.8 Hz, 2H), 3.69 (t, J = 4.8 Hz, 2H), 1.51 (s, 6H). Synthesis of 2-((2-(4-bromophenyl)propan-2-yl)oxy)ethan-l-amine

[00429] Scheme 18B 3 Zn / NH4CI EtOH, H2O 4

[00430] To a solution of l-bromo-4-(2-(2-nitroethoxy )propan-2-yl)benzene (3) (1.5 g, 3.7 mmol, 1.0 eq) inEtOH / H2O (10 mL / 2 mL) was added Zn (1.2 g, 18.5 mmol, 5.0 eq) andNH4Cl (1.0 g, 18.5 mmol, 5.0 eq). The resulting mixture was stirred for 12 h at 80°C under Ar. The reaction mixture was cooled to RT, diluted with water (50 mL), and extracted with EtOAc (3 x 60 mL). The organic layers were combined, washed with brine (100 mL), dried over anhydrous sodium sulfate, and concentrated to give 2-((2-(4-bromophenyl)propan-2-yl)oxy)ethan-l-amine (4) (1.2 g, crude) LC-MS: 258.0, 260.0 [M+H]+. Synthesis of methyl (2-((2-(4-bromophenyl)propan-2-yl)oxy)ethyl)carbamate

[00431] Scheme 18C O 5

[00432] To a solution of 2-((2-(4-bromophenyl)propan-2-yl)oxy)ethan-l-amine (4) (1.2 g, 4.7 mmol, 1.0 eq) in DCM (10 mL) was added TEA (707 mg, 7.0 mmol, 1.5 eq), followed by the addition of methyl carbonochloridate (5) (395 mg, 4.2 mmol, 0.9 eq). The mixture was stirred at RT for one day. The reaction was concentrated and the residue was purified by column chromatography on silica gel eluted with 0-30% EtOAc / hexane to afford methyl (2-((2-(4-bromophenyl)propan-2-yl)oxy)ethyl)carbamate (6) (1.0 g). LC-MS: 357.0, 359.0 [M+H+MeCN]+. Synthesis of methyl (2-((2-(4-cyanophenyl)propan-2-yl)oxy)ethyl)carbamate

[00433] Scheme 18D

[00434] To a solution of methyl (2-((2-(4-bromophenyl)propan-2-yl)oxy)ethyl)carbamate (6) (1.0 g, 3.7 mmol, 1 eq) in NMP (10 mL) was added Zn(CN)2 (260 mg, 2.2 mmol, 0.6 eq), followed by Pd(PPh3)4 (430 mg, 0.37 mmol, 0.1 eq). The resulting solution was stirred for 2 h at 140°C under Ar. The resulting mixture was cooled to RT, diluted with water (50 mL), and extracted with EtOAc (3 x 20 mL). The organic layers were combined, washed with brine (100mL), dried over anhydrous sodium sulfate, and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-40% EtOAc / hexane to give methyl (2-((2-(4-cyanophenyl)propan-2-yl)oxy)ethyl)carbamate (7) (600 mg). ^NMR (400 MHz, DMSO): 5 7.86-7.76 (m, 2H), 7.64-7.58 (m, 2H), 7.25-7.11 (m, 1H), 3.33 (s, 3H), 3.15-3.04 (m, 4H), 1.46 (s, 6H). Synthesis of methyl (2-((2-(4-carbamimidoylphenyl)propan-2-yl)oxy)ethyl)carbamate

[00435] Scheme 18E 7 8

[00436] To a solution of methyl (2-((2-(4-cyanophenyl)propan-2-yl)oxy)ethyl)carbamate (7) (0.6 g, 2.7 mmol, 1.0 eq) in MeOH (5 mL) was added MeONa (295 mg, 5.5 mmol, 2.0 eq); the mixture was stirred at RT for 4 h. NH4CI (287 mg, 5.5 mmol, 2.0 eq) was added and the mixture was stirred at 40°C for 16 h. The mixture was cooled to RT, filtered, and the filtrate was concentrated to give methyl (2-((2-(4-carbamimidoylphenyl)propan-2-yl)oxy)ethyl)carbamate (8) (285 mg, crude). LC-MS: 280.2 [M+H]+.

[00437] Scheme 18F O O Compound 18

[00438] To a solution of methyl (2-((2-(4-carbamimidoylphenyl)propan-2-yl)oxy)ethyl)carbamate (8) (285 mg, 0.7 mmol, 1.0 eq) in MeOH (5 mL) was added K2CO3 (180 mg, 1.3 mmol, 2.0 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (80 mg, 0.5 mmol, 0.7 eq) under N2. The mixture was stirred at 70°C for 2 h, and then concentrated and purified by Prep-HPLC to give methyl (2-((2-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)propan-2-yl)oxy)ethyl)carbamate (Compound 18). LC-MS: 404.2 [M+H]+. 'H NMR (400 MHz, DMSO): 5 12.71 (s, 1H), 8.05 (d, J = 8.2 Hz, 2H), 7.56 (d, J = 8.4 Hz, 2H), 7.16 (s, 1H), 3.51 (m, 5H), 3.12 (dd, J = 12.5, 5.0 Hz, 4H), 2.89 (d, J = 4.0 Hz, 4H), 1.47 (s, 6H). Compound 19 Synthesis of 2-(6'-bromo-[2,3'-bipyridin]-5-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 19) O Synthesis of 6'-bromo-[2,3'-bipyridine]-5-carbonitrile

[00439] Scheme 19A

[00440] To a solution of 6-bromonicotinonitrile (1) (2.00 g, 10.93 mmol, 1.0 eq) and (6-brom opyri din-3-yl)boronic acid (la) (2.00 g, 9.91 mmol, 9.07e-l eq) in dioxane (20 mL) / H2O (4 mL) was added K2CO3 (3.02 g, 21.86 mmol, 2.0 eq) and Pd(dppf)C12 (799.65 mg, 1.09 mmol, 0.1 eq) at 20°C under N2. The mixture was stirred at 100°C for 16 h under N2. After cooling to 25°C, the mixture was filtered and then the filtrate was concentrated under reduced pressure to give a residue that was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 50 / 1 to 0 / 1). 6'-bromo-[2,3'-bipyridine]-5-carbonitrile (2) (730 mg) was obtained. ’HNMR: (400 MHz, CDCI3): 3 = 8.92 (dd, J = 1.8, 12.3 Hz, 2H), 8.19 (dd, J = 2.5, 8.3 Hz, 1H), 8.00 (dd, J = 2.0, 8.3 Hz, 1H), 7.81 (d, J = 8.3 Hz, 1H), 7.58 (d, J = 8.3 Hz, 1H). Synthesis of 6'-bromo-[2,3'-bipyridine]-5-carboximidamide

[00441] Scheme 19B 2                                                     3

[00442] To a solution of 6'-bromo-[2,3'-bipyridine]-5-carbonitrile (2) (280 mg, 1.08 mmol, 1.0 eq) in THF (3 mL) was added lithium bis(trimethylsilyl)amide (LiHMDS) (1 M, 2.69 mL, 2.5 eq) at 0°C under N2. The mixture was warmed to 20°C and stirred at 20°C for 12 h. The reaction mixture was quenched by addition of HCl / dioxane (4 mol / L, 1 mL) at 0°C and then concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (7 mL), and the mixture stirred at 20°C for 1 hr. Then the mixture was filtered, and the filter cake was dried under reduced pressure to give a 6'-bromo-[2,3'-bipyridine]-5-carboximidamide (3) (350 mg, crude, HC1) and used immediately in the next step.

[00443] Scheme 19C 3                                                   Compound 19

[00444] To a solution of 6'-bromo-[2,3'-bipyridine]-5-carboximidamide (3) (350 mg, 1.12 mmol, 1.0 eq, HC1) in MeOH (3.5 mL) was added methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3a) (194.45 mg, 1.12 mmol, 1.0 eq) and K2CO3 (462.79 mg, 3.35 mmol, 3.0 eq) at 20°C. The mixture was stirred at 60°C for 16 h. The mixture was filtered, and concentrated under reduced pressure to give a residue that was purified by prep-HPLC (neutral condition; column: Phenomenex C18 75 * 30mm * 3 pm; mobile phase: [water (NH4HCO3)-ACN]; B%: 25%-40%, 10 mins). 2-(6'-bromo-[2,3'-bipyridin]-5-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 19) was obtained. LC-MS: 401 (M+l). ’HNMR: (400 MHz, DMSO-d6): 3 = 9.27 (d, J = 1.3 Hz, 1H), 8.94 (d, J = 2.2 Hz, 1H), 8.43 (dd, J = 1.9, 8.3 Hz, 1H), 8.27 (dd, J= 2.3, 8.4 Hz, 1H), 7.90 (d, J = 8.3 Hz, 1H), 7.61 (d, J= 8.4 Hz, 1H), 3.28 (s, 2H), 2.61 (br dd, J = 5.0, 15.4 Hz, 4H). Compound 20 Synthesis of 2-(4-(6-bromopyridin-3-yl)-3-jluorophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 20) O

[00445] Scheme 20A OH HO'® Dioxane / H2O (5:1) 20-100°C, 16 hrs Br 1a (1.0 eq) Pd(dppf)CI2 (0.1 eq), K2CO3 (2.0 eq)

[00446] To a solution of (6-bromopyridin-3-yl)boronic acid (la) (408.52 mg, 2.02 mmol, 1.0 eq) in dioxane (5 mL) / H20 (1 mL) was added 3-fluoro-4-iodobenzonitrile (1) (0.5 g, 2.02 mmol, 1.0 eq), K2CO3 (559.52 mg, 4.05 mmol, 2.0 eq), and Pd(dppf)C12 (148.11 mg, 202.42 pmol, 0.1 eq) at 20 °C under N2. The mixture was stirred at 100 °C for 16 h under N2. After cooling to 20 °C, the mixture was filtered, and concentrated under reduced pressure to give a residue that was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 20 / 1 to 0 / 1). 4-(6-bromopyridin-3-yl)-3-fluorobenzonitrile (2) (200 mg, crude) was obtained and used as is in the next step. 'HNMR: (400 MHz, CDCI3): b = 8.48 (s, 1H), 7.68 (td, J= 1.9, 8.3 Hz, 1H), 7.56 (s, 1H), 7.50 (br d, J= 7.5 Hz, 2H), 7.47 - 7.45 (m, 1H). Synthesis of 4-(6-bromopyridin-3-yl)-3-jluorobenzimidamide 2                                                     3

[00448] To a solution of 4-(6-bromopyridin-3-yl)-3-fluorobenzonitrile (2) (200 mg, 721.78 pmol, 1.0 eq) in THF (2 mL) was added LiHMDS (1 M, 1.80 mL, 2.5 eq) at 0 °C under N2. The mixture was warmed to 20 °C and stirred at 20 °C for 16 h. The reaction mixture was quenched by addition of HCl / dioxane (4 mol / L, 0.6 mL) at 0 °C and then concentrated under reduced pressure to give a residue that was suspended in MeOH (3 ml), and the mixture stirred at 20°C for 1 h. Then the mixture was filtered, and the filter cake was dried under reduced pressure to give 4-(6-bromopyridin-3-yl)-3-fluorobenzimidamide (3) (0.11 g, crude) that was used in the next step without further purification.

[00449] Scheme 20C O'" 3                                              Compound 20

[00450] To a solution of methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3a) (68.75 mg, 394.62 pmol, 1.06 eq) and 4-(6-bromopyridin-3-yl)-3-fluorobenzimidamide (3) (0.11 g, 373.99 pmol, 1.0 eq) in MeOH (2 mL) was added K2CO3 (183.33 mg, 1.33 mmol, 3.55 eq) at 20°C. The mixture was stirred at 60°C for 16 h. After cooling to 20°C, the mixture was filtered, and then the filtrate was concentrated under reduced pressure to give a residue that was purified by prep-HPLC (neutral condition; column: Waters Xbridge Prep OBD C18 150 * 40 mm * 10 pm; mobile phase: [water (NH3H2O+NH4HCO3)-ACN]; B%: 30%-60%, 8 mins). 2-(4-(6-bromopyridin-3-yl)-3-fluorophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 20) was obtained. LC-MS: 417 + bromo isomers (M+l). ^NMR: (400 MHz, DMSO-d6): 3 8.66 (s, 1H), 8.13 - 8.06 (m, 2H), 8.06 - 8.00 (m, 1H), 7.86 - 7.77 (m, 2H), 3.55 (s, 2H), 2.91 (s, 4H). Compound 21 Synthesis of 2-(4-(6-bromopyridin-3-yl)-3,5-difluorophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 21) O Synthesis of 3,5-difluoro-4-iodobenzonitrile

[00451] Scheme 21A F LDA(1.12eq), l2 (1.05 eq) THF, -70~ 15°C, 1 hr

[00452] To a solution of 3,5-difluorobenzonitrile (1) (2.00 g, 14.07 mmol, 1.0 eq) in THF (20 mL), lithium diisopropylamide (LDA) (2 M, 7.86 mL, 1.12 eq) was added dropwise at -70°C. Then b (3.76 g, 14.80 mmol, 2.98 mL, 1.05 eq) in THF (10 mL) at -70°C was added at -70°C. The reaction mixture was slowly warmed to 15°C then stirred at 15°C for 1 hr. The mixture was quenched with 10% sodium thiosulfite solution (15 ml). The reaction mixture was extracted with a 1 / 1 mixture of ethyl acetate / hexanes (7 mL x 3). The combined organic phase was dried over Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC (neutral condition; column: Welch Xtimate C18 250 * 70 mm # 10 pm; mobile phase: [water (NH4HCO3)-ACN]; B%: 40%-70%, 20 mins). 3,5-difluoro-4-iodobenzonitrile (2) (2.00 g) was obtained. 'HNMR: (400 MHz, CDCI3): 3 = 7.23 - 7.18 (m, 2H). Synthesis of 4-(6-bromopyridin-3-yl)-3,5-dijluorobenzonitrile NC^^^F iGe Br

[00453] Scheme 2IB 15-100°C, 16 hrs 2                                                           3

[00454] To a solution of (6-bromopyridin-3-yl)boronic acid (2a) (1.60 g, 7.93 mmol, 1.05 eq) and 3,5-difluoro-4-iodobenzonitrile (2) (2.00 g, 11.32 mmol, 1.0 eq) in dioxane (20 mL) / H20 (4 mL) was added K2CO3 (2.09 g, 15.09 mmol, 2.0 eq) and Pd(dppf)C12 (552.23 mg, 0.75 mmol, 0.1 eq) at 15°C under N2. The mixture heated to 100°C for 16 h under N2. After cooling to 15°C, the mixture was filtered, and then the filtrate was concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 50 / 1 to 0 / 1). 4-(6-bromopyridin-3-yl)-3,5-difluorobenzonitrile (3) (620 mg, 210 mmol) was obtained. 'H NMR: (400 MHz, CDC13): ¢5 = 8.43 (s, 1H), 7.63 -7.55 (m, 2H), 7.34 - 7.26 (m, 2H). Synthesis of 4-(6-bromopyridin-3-yl)-3,5-dijluorobenzimidamide NH2

[00456] To a solution of 4-(6-bromopyridin-3-yl)-3,5-difluorobenzonitrile (3) (500 mg, 1.69 mmol, 1.0 eq) in THF (5 mL) was added LiHMDS (1 M, 4.24 mL, 2.5 eq) at 0°C under N2. The mixture was warmed to 20°C and stirred for 16 h. The reaction mixture was quenched by addition of HCl / dioxane (4 mol / L, 2.0 mL) at 0°C, and then concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (8 ml), and the mixture was stirred at 20°C for 1 hr. Then the mixture was filtered, and the filtrate was concentrated under reduced pressure to give 4-(6-bromopyridin-3-yl)-3,5-difluorobenzimidamide (4) (540 mg, crude, HC1). The compound was used immediately in the next step.

[00458] To a solution of 4-(6-bromopyridin-3-yl)-3,5-difluorobenzimidamide (4) (440 mg, 1.41 mmol, 1.0 eq) in MeOH (4.4 mL) was added methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (4a) (245.60 mg, 1.41 mmol, 1.0 eq) and K2CO3 (584.52 mg, 4.23 mmol, 3.0 eq) at 20°C. The mixture was stirred at 20°C for 16 h. The mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (neutral condition; column: NP-1; mobile phase: [Heptane-EtOH]; B%: 10%-70%, 10      mins).       2-(4-(6-bromopyri din-3 -y 1) - 3,5 -difluorophenyl)-3,5,7,8-tetrahy dro-4H- thiopyrano[4,3-d]pyrimidin-4-one (Compound 21) was obtained. LC-MS: 438 + bromo isomers (M+l). 'HNMR: (400 MHz, DMSO-d6): b = 13.06 - 12.89 (m, 1H), 8.64 (s, 1H), 8.10 - 8.00 (m, 3H), 7.93 (d, J= 8.4 Hz, 1H), 3.62 (s, 2H), 2.98 (s, 4H). Compound 22 Synthesis of (6-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)pyridin-3-yl)boronic acid (Compound 22) O OH Synthesis of 4-(5-bromopyridin-2-yl)benzonitrile XXn. Br

[00459] Scheme 22A OH HO ^^CN1a (1 5 eq) NC Pd(dppf)CI2 (0.1 eq), K2CO3 (2.0 eq) -----------------► Dioxane (10 vol), H2O 20-100°C, 13 hrs 1                                                                         2

[00460] To a solution of 5-bromo-2-iodopyridine (1) (0.5 g, 1.76 mmol, 1.0 eq) and (4-cyanophenyl)boronic acid (la) (388.19 mg, 2.64 mmol, 1.5 eq) in dioxane (5 mL) / H20 (1 mL) was added Pd(dppf)C12 (128.87 mg, 176.12 pmol, 0.1 eq) andK2COs (486.83 mg, 3.52 mmol, 2.0 eq) at 20°C under N2. The mixture was stirred at 100°C for 13 h. After cooling to 20°C, the mixture was filtered, and then the filtrate was concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 50 / 1 to 0 / 1). 4-(5-bromopyridin-2-yl)benzonitrile (2) (400 mg, crude) was obtained and used immediately in the next step. 'H NMR: (400 MHz, CDCI3): 3 = 8.71 (d, J = 2.1 Hz, 1H), 8.06 - 8.00 (m, 2H), 7.87. Synthesis of 4-(5-bromopyridin-2-yl)benzimidamide

[00462] To a solution of 4-(5-bromopyridin-2-yl)benzonitrile (2) (100 mg, 385.95 pmol, 1.0 eq) in THF (1 mL) was added LiHMDS (1 M, 964.87 uL, 2.5 eq) at 0°C under N2. The mixture was warmed to 20°C and stirred for 16 h. The reaction mixture was quenched by addition of HCl / dioxane (4 mol / L, 0.3 mL) at 0°C, and then concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (2 ml), and the mixture was stirred at 20°C for 1 h. Then the mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. 4-(5-bromopyridin-2-yl)benzimidamide (3) (110 mg, crude, HC1 salt) was obtained and used in the next step without further purification. Synthesis of 2-(4-(5-bromopyridin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3- d]pyrimidin-4-one

[00463] Scheme 22C 3                                                             4

[00464] To a solution of 4-(5-bromopyridin-2-yl)benzimidamide (3) (100 mg, 362.15 pmol, 1.0 eq) in MeOH (1 mL) was added methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3a) (126.18 mg, 724.29 pmol, 2.0 eq) and K2CO3 (100.10 mg, 724.29 pmol, 2.0 eq) at 20°C. The mixture was stirred at 60°C for 16 h. The mixture was filtered, and the filter cake was dried under reduced pressure to give 2-(4-(5-bromopyridin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (238 mg, crude) that was used immediately in the next step. 'H NMR: (400 MHz, CDCI3): 3 = 8.75 - 8.69 (m, 1H), 8.14 - 8.04 (m, 4H), 7.90 - 7.83 (m, 1H), 7.68 - 7.61 (m, 1H), 3.65 - 3.62 (m, 2H), 3.04 - 2.97 (m, 2H), 2.92 - 2.86 (m, 2H).

[00465] Scheme 22D 0 4                                                      Compound 22

[00466] To a solution of 2-(4-(5-bromopyridin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (238 mg, 594.57 pmol, 1.0 eq) in dioxane (3 mL) was added B2Pin2 (181.18 mg, 713.48 pmol, 1.2 eq), KOAc (175.06 mg, 1.78 mmol, 3.0 eq), and Pd(dppf)C12.DCM (48.55 mg, 59.46 pmol, 0.1 eq) under N2. The mixture was stirred at 80°C for 13 h. After cooling to 25°C, the mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (neutral condition; column: Waters Xbridge Prep OBD Cl8 150 * 40mm * 10pm; mobile phase: [water (NH4HCO3)-ACN]; B%:   15%-45%, 8 mins). (6-(4-(4-oxo-3,5,7,8-tetrahydro-4H- thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)pyridin-3-yl)boronic acid (Compound 22) was obtained. LC-MS: 366.1 (M+l). ^NMR: (400 MHz, DMSO-d6): 3 = 12.83 (br s, 1H), 9.01 (s, 1H), 8.41 (s, 2H), 8.30 - 8.20 (m, 5H), 8.06 (d, J= 8.0 Hz, 1H), 3.55 (s, 2H), 2.92 (s, 4H). Compound 23 Synthesis of 2-(4-(2-bromopyridin-4-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 23) 0

[00467] Scheme 23A OH HO'By^ kACN 1a (1.1 eq) Pd(dppf)CI2 (0.1 eq), K2CO3 (2.0 eq) || J ii nr ----------------------------------► \->N             dioxane / H2O (5:1)                           Il I 20-100°C, 12 hrs 1                                                                    2

[00468] To a solution of 2-bromo-4-iodopyridine (1) (2.50 g, 8.81 mmol, 1.00 eq) and (4-cyanophenyl)boronic acid (la) (1.42 g, 9.69 mmol, 1.10 eq) in dioxane (25.0 mL) and H2O (5.00 mL) was added K2CO3 (2.43 g, 17.6 mmol, 2.00 eq) at 20°C. The suspension was degassed and purged three times with N2. Then to the mixture was added Pd(dppf)C12 (644 mg, 881 pmol, 0.10 eq) at 20°C under N2. The suspension was degassed and purged three times with N2. The mixture was stirred under N2 at 100°C for 12 h. The reaction mixture was diluted with H2O (10.0 mL) and extracted with EtOAc 60.0 mL (20.0 mL x 3). The combined organic layers were dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 10 / 1 to 1 / 1). 4-(2-bromopyridin-4-yl)benzonitrile (2) (950 mg) was obtained. 'H NMR: (400 MHz, DMSO-d6): 3 = 8.57 (d, J = 5.3 Hz, 1H), 8.17 - 8.03 (m, 5H), 7.91 (dd, J = 1.7, 5.2 Hz, 1H). Synthesis of 4-(2-bromopyridin-4-yl)benzimidamide NH2 2                                                     3

[00470] To a solution of 4-(2-bromopyridin-4-yl)benzonitrile (2) (400 mg, 1.54 mmol, 1.00 eq) in THF (4.00 mL), LiHMDS (1 M, 3.09 mL, 2.00 eq) was added dropwise at 0°C. The mixture was stirred at 20°C for 16 h. The reaction mixture was diluted with 4 N HC1 (4.00 mL). The combined organic layers were concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (20.0 mL) and stirred at 20°C for 1 hr. Then the mixture was filtered. The filtrate was concentrated under reduced pressure to give a residue. 4-(2-bromopyridin-4-yl)benzimidamide (3) (500 mg, crude) was obtained and used in the next step without further purification.

[00471] Scheme 23C 3                                             Compound 23

[00472] To a solution of 4-(2-bromopyridin-4-yl)benzimidamide (3) (250 mg, 905 pmol, 1.00 eq) and methyl 4-oxotetrahydro-2H-thiopyran-3-carboxylate (3a) (166 mg, 951 pmol, 1.05 eq) in MeOH (2.50 mL) was added K2CO3 (313 mg, 2.26 mmol, 2.50 eq) at 20°C. The mixture was stirred at 60°C for 16 h. The reaction mixture was filtered. The filter cake was slurried by MeOH (0.50 mL) and H2O (1.00 mL) and then filtered. The filter cake was dried in vacuo to give a residue. The residue was purified by prep-HPLC (column: Phenomenex luna C18 80 * 40 mm * 3 pm; mobile phase: [water (HCl)-ACN]; B%: 35%-65%, 7 min). 2-(4-(2- bromopyridin-4-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 23) was obtained. 'HNMR (400 MHz, DMSO-d6): 3 = 8.48 (d, J = 5.3 Hz, 1H), 8.27 (d, J = 8.4 Hz, 2H), 8.07 (s, 1H), 7.99 (d, J = 8.4 Hz, 2H), 7.87 (dd, J = 1.2, 5.2 Hz, 1H), 3.54 (s, 2H), 2.89 (s, 4H). LC-MS: 404.1 (M+l). Compound 24 Synthesis of (2-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)pyrimidin-5-yl)boronic acid (Compound 24) 1                                                                      2

[00474] To a solution of 5-bromo-2-iodopyrimidine (1) (2.50 g, 8.78 mmol, 1.00 eq) and (4-cyanophenyl)boronic acid (la) (1.42 g, 9.65 mmol, 1.10 eq) in dioxane (25.0 mL) and H2O (5.00 mL) was added K2CO3 (2.43 g, 17.6 mmol, 2.00 eq) at 15°C. The suspension was degassed and purged three times with N2. Then to the mixture was added Pd(dppf)C12 (642 mg, 878 pmol, 0.10 eq) at 15°C under N2. The suspension was degassed and purged three times with N2. The mixture was stirred under N2 at 100°C for 16 h. The reaction mixture was diluted with H2O (10.0 mL) and filtered. The filtrate was extracted with EtOAc (10 mL x 2). The combined organic layers were dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue. The residue was suspended in MTBE (10.0 mL), stirred at 15°C for 16 h, then filtered. The filter cake was dried under reduced pressure to give a residue. 4-(5-bromopyrimidin-2-yl)benzonitrile (2) (2.00 g, crude) was obtained and used immediately in the next step. 'H NMR (400 MHz, DMSO-d6): 3 = 9.16 (s, 2H), 8.49 (br d, J = 8.3 Hz, 2H), 8.01 (br d, J = 8.3 Hz, 2H). Synthesis of 4-(5-bromopyrimidin-2-yl)benzimidamide NH2

[00476] To a solution of 4-(5-bromopyrimidin-2-yl)benzonitrile (2) (0.80 g, 3.08 mmol, 1.00 eq) in THF (8.00 mL) was added LiHMDS (1 M, 6.15 mL, 2.00 eq) at 0 °C. The mixture was stirred at 15°C for 16 h. The reaction mixture was diluted with 4 N HCl / dioxane (6.00 mL). The combined organic layers were concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (8.00 mL) and stirred at 15°C for 2 h, then filtered. The filtrate was concentrated under reduced pressure to give a residue. 4-(5-bromopyrimidin-2-yl)benzimidamide (3) (0.8 g, crude) was obtained which was used in the next step without further purification. Synthesis of 2-(4-(5-bromopyrimidin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00477] Scheme 24C 6(1.05 eq) K2CO3 (3.0 eq), MeOH 25-60°C, 16 hrs 3                                                              4

[00478] To a solution of 4-(5-bromopyrimidin-2-yl)benzimidamide (3) (0.80 g, 2.89 mmol, 1.00 eq) and methyl 4-oxotetrahydrothiopyran-3-carboxylate (6) (528 mg, 3.03 mmol, 1.05 eq) in MeOH (16.0 mL) was added K2CO3 (1.20 g, 8.66 mmol, 3.00 eq) at 25°C. The mixture was stirred at 60°C for 16 h. The reaction mixture was filtered. The filtrate was concentrated under reduced pressure. The resulting solid was suspended in water (5 mL) and stirred at 20°C for 3 h. Then the mixture was filtered, and the filter cake was dried in vacuo to give 2-(4-(5-bromopyrimidin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (300 mg, crude). The crude product was used in the next step directly without further purification.

[00479] Scheme 24D o Compound 24

[00480] To a solution of 2-(4-(5-bromopyrimidin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (0.1 g, 249.20 pmol, 1 eq) in dioxane (2 mL) was added 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-l,3,2-dioxaborolane (69.61 mg, 274.12 pmol, 1.1 eq), KO Ac (48.91 mg, 498.41 pmol, 2 eq), and Pd(dppf)C12.CH2C12 (20.35 mg, 24.92 pmol, 0.1 eq) at 20°C under N2. The mixture was stirred at 80°C for 12 h under N2. The mixture was concentrated under reduced pressure to give a residue. The residue was suspended in MeCN / H2O (1:1, 3 mL) and stirred at 20°C for 2 h. Then the mixture was filtered, and the filter cake was dried in vacuo to give 2-(4-(5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyrimidin-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (50 mg, crude). The crude product was suspended in HCI / H2O (4 N, 3 mL) and stirred at 20°C for 12 h. Then the mixture was diluted with DMSO (5 mL) and purified by prep-HPLC (column: Phenomenex luna C18 250 * 50 mm * 10 pm; mobile phase: [water (HCl)-ACN]; B%: 15%-45%, 10 min). (2-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)pyrimidin-5-yl)boronic acid (Compound 24) was obtained. LCMS: 367 (M+l). 'H NMR: (400 MHz, DMSO-d6): 5 = 9.16 (s, 2H), 8.54 (d, J = 8.5 Hz, 2H), 8.25 (d, J = 8.5 Hz, 2H), 3.55 (s, 2H), 2.92 (s, 4H). Compound 25 Synthesis of (3',5'-difluoro-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-yl)-[l,l'-biphenyl]-4-yl)boronic acid (Compound25) O OH Synthesis of 4 '-bromo-3,5-difluoro-[1,1 '-biphenyl]-4-carbonitrile

[00481] Scheme 25A 1                                                                      2

[00482] To a mixture of 2,6-difluoro-4-iodobenzonitrile (1) (5 g, 22.94 mmol, 1 eq) and 2-(4-bromophenyl)-4,4,5,5-tetramethyl-l,3,2-dioxaborolane (la) (6.49 g, 22.94 mmol, 1 eq) in dioxane (100 mL) and H2O (10 mL) was added K2CO3 (6.34 g, 45.87 mmol, 2 eq) and Pd(PPh3)2Cl2 (1.61 g, 2.29 mmol, 0.1 eq) at 20°C under N2. The mixture was degassed and purged three times with N2, and then the mixture was stirred at 110°C for 16 h under an N2 atmosphere. The mixture was cooled to 20°C and then diluted with water (100 mL). Then the mixture was extracted with EtOAc 300 mL (100 mL x 3). The combined organic layers were washed with brine (100 mL), dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue that was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 50 / 1 to 0 / 1). 4'-bromo-3,5-difluoro-[l,r-biphenyl]-4-carbonitrile (2) (1.8g) was obtained. 'H NMR: (400 MHz, CDC13): 8 = 7.57 (d, J = 8.6 Hz, 2H), 7.39 - 7.33 (m, 2H), 7.20-7.17 (m, 2H). Synthesis of 4'-bromo-3,5-difluoro-[1,1 '-biphenyl]-4-carboximidamide NH F "A.

[00483] Scheme 25B 2                                                          3

[00484] To a solution of 4'-bromo-3,5-difluoro-[l,r-biphenyl]-4-carbonitrile (2) (0.5 g, 1.70 mmol, 1 eq) in THF (5 mL), LiHMDS (1 M, 4.25 mL, 2.5 eq) was added dropwise at 0°C. The mixture was stirred at 15°C for 16 h. The reaction mixture was quenched by dropwise addition of 4 N HCl / dioxane (10 mL). The mixture was concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (10 mL) and stirred at 20°C for 1 hr. Then the mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. 4'-bromo-3,5-difluoro-[l,r-biphenyl]-4-carboximidamide (3) (528 mg, crude) was obtained which was used in the next step without further purification. Synthesis of 2-(4'-bromo-3,5-difluoro-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00485] Scheme 25C NH F OMe (1.5 eq) K2CO3 (2.0 eq), MeOH 25°C, 16 hrs

[00486] To a mixture of 4'-bromo-3,5-difluoro-[l,T-biphenyl]-4-carboximidamide-HCl (3) (528 mg, 1.52 mmol, 1 eq) in MeOH (10 mL) was added K2CO3 (419.88 mg, 3.04 mmol, 2 eq) and methyl 4-oxotetrahydrothiopyran-3-carboxylate (396.97 mg, 2.28 mmol, 1.5 eq) at 25°C under N2. Then the mixture was stirred at 25°C for 16 h under an N2 atmosphere. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. The residue was triturated with water (10 mL) at 25°C for 1 hr and then filtered. The filter cake was dried in vacuo to give 2-(4'-bromo-3,5-difluoro-[l,r-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (0.9 g, crude) which was used directly in the next step. Synthesis of 2-(3,5-difluoro-4'-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-[1,1 '-biphenyl / -4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00487] Scheme 25D o

[00488] To a mixture of 2-(4'-bromo-3,5-difluoro-[l,r-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (150 mg, 344.60 pmol, 1 eq) in dioxane (3 mL) was added B2Pin2 (131.26 mg, 516.90 pmol, 1.5 eq) and KOAc (101.46 mg, 1.03 mmol, 3 eq) at 25°C. The mixture was degassed and purged three times with N2 and then Pd(dppf)C12 (28.14 mg, 34.46 pmol, 0.1 eq) was added at 25°C under N2. The mixture was stirred at 100°C for 16 h under a N2 atmosphere. After cooling to 20°C, the reaction mixture was filtered and concentrated under reduced pressure to give a residue. The crude product was triturated with water (2 mL) and acetonitrile (2 ml) at 25°C for 1 hr. The mixture was filtered, and the filter cake was dried in vacuo. 2-(3,5-difluoro-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (120 mg) was obtained.

[00489] Scheme 25E 5                                                  Compound 25

[00490] To a stirred solution of HCI / H2O (4 M, 2 mL), 2-(3,5-difluoro-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,r-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (120 mg, 248.78 pmol, 1 eq) was added portion-wise at 20°C under N2. Then the mixture was stirred at 50°C for 2 h under an N2 atmosphere. The mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Waters XbridgeBEH Cl8 100*30mm* 10 pm; mobile phase: [water (NH4HCO3)-ACN]; B%: 25%-55%, 8 min). (3',5'-difluoro-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid (Compound 25) was obtained. LCMS: 401 (M+l). 'H NMR: (400 MHz, DMSO-d6): 5 = 13.30 - 12.89 (m, 1H), 8.19 (s, 2H), 7.98 - 7.86 (m, 2H), 7.84 - 7.77 (m, 2H), 7.73 - 7.65 (m, 2H), 3.55 (br s, 2H), 2.97 -2.81 (m, 4H). Compound 26 Synthesis of (3'-hydroxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)~ [ 1,l'-biphenyl]-4-yl)boronic acid (Compound26) Synthesis of 4 '-bromo-3-methoxy-[1,1 '-biphenyl]-4-carbonitrile

[00491] Scheme 26A dioxane / H2O (5:1), 20-75°C, 16 hrs OH Br(11 ecl) K2CO3 (2.0 eq), Pd(dppf)CI2 (0.1 eq) 1                                                                    2

[00492] To a solution of 4-bromo-2-methoxybenzonitrile (1) (10.0 g, 47.2 mmol, 1.00 eq) and (4-bromophenyl)boronic acid (10.4 g, 51.9 mmol, 1.10 eq) in dioxane (50.0 mL) and H2O (10.0 mL) was added K2CO3 (13.0 g, 94.3 mmol, 2.00 eq) and Pd(dppf)C12 (3.45 g, 4.72 mmol, 0.10 eq) at 20°C. The mixture was heated to 75°C for 16 h. TLC (petroleum ether / ethyl acetate = 5 / 1, Rf = 0.53) indicated that (1) was consumed completely. The reaction mixture was diluted with EtOAc (50.0 mL) and H2O (20.0 mL). The organic layer was separated from the mixture and the aqueous phase was extracted with EtOAc (50.0 mL x 3). The combined organic layers were dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue that was purified by column chromatography (SiO2, petroleum ether / ethylacetate = 10 / 1 to 0 / 1) to provide 4'-bromo-3-methoxy-[l,l'-biphenyl]-4-carbonitrile (2) (3.30 g, 8.02 mmol). The compound was used without further purification. 1H NMR (400 MHz, DMSO-t / e): d = 7.86 (d, J= 8.1 Hz, 1H), 7.84 - 7.72 (m, 4H), 7.52 (d, J = 1.1 Hz, 1H), 7.45 (dd, J = 1.4, 8.0 Hz, 1H), 4.08 (s, 3H). Synthesis of 4 '-bromo-3-methoxy-[1,1 '-biphenyl]-4-carboximidamide NH, OMe

[00494] To a solution of 4'-bromo-3-methoxy-[l,T-biphenyl]-4-carbonitrile (2) (2.00 g, 6.94 mmol, 1.00 eq) in THF (20.0 mL), LiHMDS (1 M, 13.9 mL, 2.00 eq) was added dropwise at 0°C and then allowed to warm to 20°C and stirred for 12 h. The reaction mixture was quenched with HCl / dioxane (13.0 mL) at 0 °C, and then concentrated under reduced pressure to give a residue. The residue was triturated with MeOH (10 mL) and filtered. The filter cake was dried in vacuo to give a residue. 4'-bromo-3-methoxy-[l,l'-biphenyl]-4-carboximidamide (3) (3.00 g, crude) was obtained. The crude product was used in the next step without further purification. LC-MS: 306 (M+l). Synthesis of 2-(4'-bromo-3-methoxy-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one O OMe

[00495] Scheme 26C o                       o 3                                                                     4

[00496] To a solution of 4'-bromo-3-methoxy-[l,T-biphenyl]-4-carboximidamide (3) (3.00 g, 9.83 mmol, 1.00 eq) and methyl 4-oxotetrahydrothiopyran-3-carboxylate (3.43 g, 19.6 mmol, 2.00 eq) in MeOH (45.0 mL) was added K2CO3 (5.43 g, 39.3 mmol, 4.00 eq) at 25°C and then heated to 50°C and stirred for 16 h. LC-MS showed that (3) was consumed. The reaction mixture was filtered, and the filter cake was dried under reduced pressure to give a residue. The residue was triturated with H2O (30.0 mL) and stirred at 20°C for 16 h. Then the mixture was filtered, and the filter cake was dried under reduced pressure to give 2-(4'-bromo-3-methoxy-[l,T-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (1.5 g, crude), which was used in the next step without further purification. LC-MS: 430.2 (and bromo isomers) (M+l). Synthesis of 2-(3-methoxy-4'-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-[1,1 '-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00497] Scheme 26D 0

[00498] To a solution of 2-(4'-bromo-3-methoxy-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (1.00 g, 2.33 mmol, 1.00 eq) and Pin2B2 (650 mg, 2.56 mmol, 1.10 eq) in dioxane (50.0 mL) was added KO Ac (457 mg, 4.66 mmol, 2.00 eq) and Pd(dppf)C12.CH2C12 (190 mg, 233 pmol, 0.10 eq) at 20°C. The mixture was heated to 80°C and stirred for 16 h. LC-MS showed (4) was consumed completely and one main peak with the desired mass was detected. The mixture was filtered, and the filtrate was concentrated under reduced pressure to provide 2-(3-methoxy-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,T-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (1.60 g, crude). The crude product was used in the next step without further purification. LC-MS: 477.2 (M+l). Synthesis of (3'-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)~ [1,1 '-biphenyl]-4-yl) boronic acid

[00500] A mixture of 2-(3-methoxy-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5)   (1.60 g, 3.36 mmol, 1.00 eq) in HC1 (8 M, 32.0 mL, 76.2 eq) was heated to 80°C and stirred for 16 h. HC1 (12 M, 8.00 mL, 28.6 eq) was added to the mixture at 20°C, and then heated to 80°C for 4 h. LC-MS showed (5) was consumed completely and one main peak with the desired mass was detected. The reaction mixture was filtered, then the filter cake was dried under reduced pressure to give (3'-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,l'-biphenyl]-4-yl)boronic acid (6) (710 mg, crude). The crude product was used in the next step without further purification. LC-MS: 395.2 (M+l).

[00501] Scheme 26F o                                                 o 6         OH                                  Compound 26       OH

[00502] To a solution of (3'-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,T-biphenyl]-4-yl)boronic acid (6) (710 mg, 1.80 mmol, 1.00 eq) in DCM (21.6 mL), BBra (1 M in DCM, 9.00 mL, 5.00 eq) was added dropwise at -20°C. The mixture was heated to 20°C and stirred for Ih. LC-MS showed that (6) was consumed completely and one peak with the desired mass was detected. The reaction mixture was quenched by the addition of ice water (20.0 mL) at 0°C. The mixture was filtered, and the filter cake was dried under reduced pressure to give a residue that was purified by prep-HPLC (column: Phenomenex Gemini NXC18 (75 x 30 mm x 3 pm); mobile phase: [H2O (0.05% NH3H2O + 10 mM NH4HCO3)-ACN]; gradient: 15%-65% B over 8.0 min) to give (3'-hydroxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,T-biphenyl]-4-yl)boronic acid (Compound 26). LC-MS: 381.2 (M+l). 'H NMR (400 MHz, DMSO4): b = 8.27 (d, J= 8.1 Hz, IH), 8.13 (s, 2H), 7.90 (d, J = 8.0 Hz, 2H), 7.71 (d, J= 8.0 Hz, 2H), 7.32 - 7.19 (m, 2H), 3.55 (s, 2H), 2.93 (s, 4H). Compound 27 Synthesis of (3-hydroxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[ 1,l'-biphenyl]-4-yl)boronic acid (Compound27) Synthesis of 4 '-bromo-3'-methoxy-[1,1 '-biphenyl]-4-carbonitrile Y Y Br

[00503] Scheme 27A 1                                                                             2

[00504] To a solution of l-bromo-4-iodo-2-methoxybenzene (1) (8 g, 25.56 mmol, 1 eq) and (4-cyanophenyl)boronic acid (la) (4.51 g, 30.7 mmol, 1.20 eq) in dimethylformamide (DMF) (160 mL) was added Cs2CO3 (16.7 g, 51.1 mmol, 2.00 eq) and Pd(PPh3)4 (1.48 g, 1.28 mmol, 0.05 eq) at 25°C. The mixture was heated to 80°C and stirred for 16 h under N2. LC-MS showed that (1) was consumed completely. The reaction mixture was diluted with EtOAc and H2O. The organic layer was separated from the mixture and the aqueous phase was extracted with EtOAc. The combined organic layers were dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 10 / 1 to 0 / 1) to provide 4'-bromo-3'-methoxy-[l,l'-biphenyl]-4-carbonitrile (2) (4.50 g, 15.6 mmol). 'HNMR (400 MHz, DMSO-d6): 3 = 8.00 (s, 4H), 7.74 (d, J = 8.2 Hz, 1H), 7.46 (d, J = 2.0 Hz, 1H), 7.31 (dd, J = 2.1, 8.2 Hz, 1H), 4.02 (s, 3H). Synthesis of 4 '-bromo-3 '-methoxy-[1,1 '-biphenyl]-4-carboximidamide

[00506] To a solution of 4'-bromo-3'-methoxy-[l,r-biphenyl]-4-carbonitrile (2) (2.00 g, 6.94 mmol, 1.00 eq) in THF (20.0 mL), was added dropwise LiHMDS (1 M, 13.9 mL, 2.00 eq) at 0°C. The mixture was warmed to 25°C and stirred for 12h. LC-MS showed that (2) was consumed completely. The reaction mixture was quenched by dropwise addition of HCl / dioxane (4M, 8.00 mL) at 0°C, then concentrated under reduced pressure to give a residue. The residue was suspended in MeOH (20.0 mL), and the mixture stirred at 25°C for 1 h. Then the mixture was filtered, and the filtrate was concentrated under reduced pressure to give 4'-bromo-3'-methoxy-[l,T-biphenyl]-4-carboximidamide (3) (3.00 g, crude) and used directly in the next step without further purification. LC-MS: 306.1 + bromo isomers (M+l). Synthesis of 2-(4'-bromo-3'-methoxy-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00508] To a solution of 4'-bromo-3'-methoxy-[l,T-biphenyl]-4-carboximidamide (3) (1.00 g, 3.28 mmol, 1.00 eq) and methyl 4-oxotetrahydrothiopyran-3-carboxylate (1.14 g, 6.55 mmol, 2 eq) in MeOH (15.0 mL) was added K2CO3 (1.81 g, 13.1 mmol, 4.00 eq) at 25°C. The mixture was heated to 50°C and stirred for 16 h. LC-MS showed (3) was consumed completely. The reaction mixture was filtered, and the filter cake was dried under reduced pressure to give a residue. H2O (10.0 mL) was added to the residue to form a slurry that was stirred at 20°C for 16h. Then the mixture was filtered, and the filter cake was dried under reduced pressure to give 2-(4'-bromo-3'-methoxy-[l,r-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (1.50 g, crude). LC-MS: 430.2 and bromo isomers (M+l). 'H NMR (400 MHz, DMSO-d6): b = 12.76 (br s, 1H), 8.26 - 8.20 (m, J = 8.4 Hz, 2H), 7.90 - 7.82 (m, J = 8.4 Hz, 2H), 7.68 (d, J = 8.3 Hz, 1H), 7.42 (d, J = 1.9 Hz, 1H), 7.27 (dd, J = 2.0, 8.3 Hz, 1H), 3.98 (s, 3H), 3.53 (s, 2H), 2.89 (s, 4H). Synthesis of 2-(3 '-methoxy-4'-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-[I,1 '-biphenyl / -4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00510] To a solution of 2-(4'-brorno-3'-rnethoxy-[l,T-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (1.00 g, 2.33 mmol, 1.00 eq) and B2Pin2 (651 mg, 2.56 mmol, 1.10 eq) in dioxane (30.0 mL) was added KO Ac (457 mg, 4.66 mmol, 2.00 eq) and Pd(dppf)C12.DCM (190 mg, 233 pmol, 0.10 eq) at 25°C. The mixture was heated to 80°C and stirred under N2 for 16h. LC-MS showed that (4) was consumed completely. The mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. 2-(3'-methoxy-4'-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (1.00 g, crude) was obtained, which was used directly in the next step without further purification. LC-MS: 477.1 (M+l). Synthesis of (3-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[1,1 '-biphenyl]-4-yl) boronic acid

[00511] Scheme 27E 5                                                           6

[00512] 2-(3'-methoxy-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (1.00 g, 2.10 mmol, 1.00 eq) was added portion-wise to a stirred solution of HCI / H2O (4 M, 20.0 mL, 38.1 eq) at 20°C. The mixture was stirred at 25°C for 5h. LC-MS showed that (5) was consumed. The reaction mixture was filtered and the filter cake dried under reduced pressure to give (3-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,T-biphenyl]-4-yl)boronic acid (6) (250 mg, crude), which was used as is in the next step. LC-MS: 395.2 (M+l).

[00513] Scheme 27F o                                             0 6         OH                                Compound 27       OH

[00514] To a solution of (3-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,T-biphenyl]-4-yl)boronic acid (6) (250 mg, 634 pmol, 1.00 eq) in DCM (7.50 mL), was added BBrs (1 M, 3.17 mL, 5.00 eq) dropwise at -20°C. The mixture was stirred at 20°C for 2h. LC-MS showed that (6) was consumed completely. The reaction mixture was cooled to 0°C and quenched with ice water (10.0 mL) and stirred at 0 °C for lOmin. The mixture was filtered and the filter cake dried under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex luna C18 100 x 40 mm x 5 pm; mobile phase: [H2O (0.04% HC1)-ACN]; gradient: 10%-50% B over 8.0 min) to provide (3-hydroxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid (Compound 27). LC-MS: 381.2 (M+l). ^NMR (400 MHz, DMSO-d6): d = 8.24 - 8.07 (m, 2H), 7.94 - 7.84 (m, 1H), 7.83 - 7.74 (m, 2H), 7.37 - 7.04 (m, 2H), 3.55 (s, 2H), 2.92 (s, 4H). Compound 28 Synthesis of (2-hydroxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[1,1'-biphenyl]-4-yl)boronic acid (Compound 28) Synthesis of 4 '-bromo-2'-methoxy-[1,1 '-biphenyl]-4-carbonitrile

[00515] Scheme 28A 1                                                                      2

[00516] To a solution of 4-bromo-l-iodo-2-m ethoxybenzene (1) (8.00g, 25.6 mmol, 1.00 eq) and (4-cyanophenyl)boronic acid (4.51g, 30.7 mmol, 1.20 eq) in DMF (80.0 mL) was added CS2CO3 (16.7g, 51.1 mmol, 2.00 eq) andPd(PPhs)4 (1.48g, 1.28 mmol, 0.05 eq) at20°C under N2. The mixture was stirred at 80°C for 14 h under N2. TLC (petroleum ether / ethyl acetate = 8 / 1, Rf = 0.54) indicated that (1) was consumed completely. The reaction mixture was diluted with EtOAc (50.0 mL) and H2O (150 mL). The layers were separated and the aqueous phase was extracted with EtOAc (50.0 mL x 3). The combined organic layers were dried over Na2SO4, filtered, and the filtrate was concentrated under reduced pressure to give a residue that was purified by column chromatography (SiO2, petroleum ether / ethyl acetate = 10 / 1 to 0 / 1) to give 4'-bromo-2'-methoxy-[l,l'-biphenyl]-4-carbonitrile (2) (6.10 g, 21.2 mmol). 'H NMR (400 MHz, DMSO-d6): b = 7.93 (d, J = 8.4 Hz, 2H), 7.73 (d, J = 8.4 Hz, 2H), 7.42 (d, J = 1.4 Hz, 1H), 7.38 - 7.30 (m, 2H), 3.92 - 3.83 (m, 3H). Synthesis of 4 '-bromo-2 '-methoxy-[1,1 '-biphenyl]-4-carboximidamide

[00518] To a solution of 4'-bromo-2'-methoxy-[l,T-biphenyl]-4-carbonitrile (2) (1.00g, 3.47 mmol, 1.00 eq) in THF (10.0 mL), LiHMDS (1 M, 6.94 mL, 2.00 eq) was added dropwise at 0°C. The mixture was stirred at 25°C for 12 h. LC-MS showed that (2) was consumed completely. The reaction mixture was quenched by dropwise addition of HCl / dioxane (6 mL) at 0°C, then concentrated under reduced pressure to give a residue that was suspended in MeOH (20 mL) and the mixture stirred at 25°C for 1 h. The mixture was filtered and the filtrate was concentrated under reduced pressure to give 4'-bromo-2'-methoxy-[l,l'-biphenyl]-4-carboximidamide (3) (2.00g, crude), which was used in the next step without further purification. LC-MS: 306.2 + bromo isomers (M+l). Synthesis of 2-(4'-bromo-2'-methoxy-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00519] Scheme 28C 3                                                               4

[00520] To a solution of 4'-bromo-2'-methoxy-[l,T-biphenyl]-4-carboximidamide (3) (1.00g, 3.28 mmol, 1.00 eq) and methyl 4-oxotetrahydrothiopyran-3-carboxylate (1.14g, 6.55 mmol, 2.00 eq) in MeOH (10 mL) was added K2CO3 (1.81g, 13.1 mmol, 4.00 eq) at 25°C. The mixture was stirred at 50°C for 16 h. LC-MS showed that (3) was consumed completely. The reaction mixture was filtered and filter cake dried under reduced pressure to give a residue. H2O (15 mL) was added to make a slurry and the mixture stirred at 20°C for 16 h. The mixture was filtered and the filter cake dried under reduced pressure to give 2-(4'-bromo-2'-methoxy-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (2.00 g, crude). The crude product was used in the next step without further purification. LC-MS: 430.3 + bromo isomers (M+l). Synthesis of 2-(2 '-methoxy-4'-(4,4,5,5-tetramethyl-l, 3,2-dioxaborolan-2-yl)-[1,1 '-biphenyl / -4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00522] To a solution of 2-(4'-bromo-2'-methoxy-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (400mg, 932 pmol, 1 eq) and B2Pin2 (260mg, 1.02 mmol, 1.10 eq) in dioxane (20 mL) was added KOAc (183mg, 1.86 mmol, 2.00 eq) and Pd(dppf)C12.DCM (76.1mg, 93.17 pmol, 0.1 eq) at 25°C. The mixture was stirred under N2 at 80°C for 16h. LC-MS showed that (4) was consumed completely. The mixture was filtered and the filtrate was concentrated under reduced pressure to give 2-(2'-methoxy-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,r-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H- thiopyrano[4,3-d]pyrimidin-4-one (5) (700 mg, crude). The crude product was used in the next step without further purification. LC-MS: 477.2 (M+l). Synthesis of (2-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)~ [1,1 '-biphenyl]-4-yl) boronic acid

[00524] 2-(2'-methoxy-4'-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-[l,l'-biphenyl]-4-yl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (5) (400mg, 840 pmol, 1.0 eq) was added portion-wise to a stirred solution of HC1 (4M, 20 mL, 95.3 eq) at 20°C. The mixture was stirred at 70°C for 16h. LC-MS showed that (5) was consumed completely. The reaction mixture was filtered, and the filtrate concentrated under reduced pressure to give (2-methoxy -4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid (6) (490 mg, crude). The crude product was used in the next step without further purification. LC-MS: 395.1 (M+l).

[00525] Scheme 28F o                                                o OH                                               OH 6                                                                 7

[00526] To a solution of (2-methoxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid (6) (200mg, 507 pmol, 1 eq) in DCM (3 mL), tribromoborane (1 M, 2.49 mL, 4.9 eq) was added dropwise at -10°C. The mixture was warmed to 20°C and stirred for 3 h. LC-MS showed that (6) was consumed completely. The reaction mixture was filtered and the filter cake dried under reduced pressure to give a residue that was purified by prep-HPLC (column: Phenomenex luna C18 100 x 40 mm x 5 pm; mobile phase: [H2O (0.04% HC1)-ACN]; gradient: 5%-45% B over 8.0 min). (2-hydroxy-4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,r-biphenyl]-4-yl)boronic acid (Compound 28) was obtained. *HNMR (400 MHz, DMSO-d6): 3 = 9.69 - 9.32 (m, 1H), 8.12 (d, J = 8.5 Hz, 2H), 7.73 (d, J = 8.4 Hz, 2H), 7.39 - 7.37 (m, 1H), 7.36 - 7.28 (m, 2H), 3.64 -3.59 (m, 2H), 2.91 (s, 4H). LC-MS: 381.1 (M+1). Compound 29 Synthesis of methyl (2-((2-(4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-2-yl)-[1,1 '-biphenyl]-4-yl)propan-2-yl)oxy)ethyl)carbamate (Compound 29) 0 Synthesis of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one O NH —^Br

[00527] Scheme 29A 1                                                                    2

[00528] To a solution of 4-bromobenzimidamide (1) (5 g, 21.2 mmol, 1.00 eq) in EtOH (50 mL) was added methyl 4-oxotetrahydrothiopyran-3-carboxylate (3.70 g, 21.2 mmol, 1.00 eq) and K2CO3 (5.87 g, 42.5 mmol, 2.00 eq) at 25°C. The mixture was stirred at 80°C for 16 h. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue. 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (7 g, crude) was obtained and used without further purification. 'H NMR: (400 MHz, DMSO-d6): 5 = 8.21 - 8.11 (m, 2H), 7.53 - 7.46 (m, 2H), 3.42 (s, 2H), 2.83 - 2.64 (m, 4H). Synthesis of 2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano [4,3-d]pyrimidin-4-one

[00529] Scheme 29B 0

[00530] To a solution of 2-(4-bromophenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (2) (7 g, 21.7 mmol, 1.00 eq) in dioxane (70 mL) was added Pin2B2 (11.0 g, 43.3 mmol, 2.00 eq), KOAc (10.6 g, 108 mmol, 5.00 eq), and Pd(dppf)C12 (1.58 g, 2.17 mmol, 0.10 eq) at 25°C under N2. The mixture was degassed three times with N2. The mixture was stirred at 100°C for 16 h. The reaction mixture was concentrated under reduced pressure to give a crude product. The crude product was triturated with H2O (150 mL) at 20°C for 2 h. The mixture was filtered, and the filter cake was concentrated under reduced pressure to give a residue. 2-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (9 g, crude) was obtained and used immediately in the next step. Synthesis of methyl (2-((2-(4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[1,1 '-biphenyl]-4-yl)propan-2-yl)oxy)ethyl)carbamate

[00531] To a solution of 2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (0.8 g, 2.16 mmol, 1.00 eq) in dioxane (16 mL) was added methyl (2-((2-(4-bromophenyl)propan-2-yl)oxy)ethyl)carbamate (4) (820 mg, 2.59 mmol, 1.20 eq), Pd(dppf)C12 (158 mg, 216 pmol, 0.10 eq), and K2CO3(1.49 g, 10.8 mmol, 5eq) at 25°C under N2. The mixture was degassed three times with N2. The mixture was stirred at 85°C for 16 h. The reaction mixture was concentrated under reduced pressure and the resultant residue diluted with H2O (20 mL) and extracted with ethyl acetate 60 mL (20 mL x 3). The combined organic layers were washed with brine (30 mL x 1), dried over Na2SO4, filtered, and concentrated under reduced pressure. The residue was purified by prep-HPLC (column: Phenomenex Luna C18 75 * 30 mm * 3 pm; mobile phase: [NaHCCL-ACN]; gradient: 40%-70% B over 16.0 min). Methyl (2-((2-(4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,l'-biphenyl]-4-yl)propan-2-yl)oxy)ethyl)carbamate (Compound 29) was obtained. LCMS: 480.1 (M+l). 'H NMR: (400 MHz, DMSO-d6): 5 = 8.19 (d, J = 8.4 Hz, 2H), 7.82 (d, J = 8.5 Hz, 2H), 7.72 (d, J = 8.4 Hz, 2H), 7.57 - 7.47 (m, 2H), 7.17 - 7.11 (m, 1H), 3.53 (s, 2H), 3.49 (s, 3H), 3.18 - 3.08 (m, 4H), 2.90 (br s, 4H), 1.49 (s, 6H). Compound 30 Synthesis of (2-hydroxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 30) Compound 32 BBr3 (5.0 eq) DCM, 0-25°C, 16 hrs 0 OH Compound 30

[00533] The synthesis of (2-methoxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3- d]pyrimidin-2-yl)phenyl)boronic acid (Compound 32) is shown in Schemes 32A-D.

[00534] To a solution of (2-methoxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 32) (0.30 g, 943 pmol, 1.00 eq) in DCM (3 mL), was added BBn (1.18 g, 4.71 mmol, 454 pL, 5.00 eq) dropwise at 0°C and then stirred at 25°C for 16h. LC-MS showed that (Compound 32) was consumed. The reaction mixture was quenched by dropwise addition of H2O (5 mL at 25°C) and extracted with ethyl acetate (3 mL x 3). The combined organic layers were washed and concentrated under reduced pressure to give a residue that was purified by prep-HPLC (column: Phenomenex Luna C8 250 x 50 mm x 10 pm; mobile phase: [H2O (0.04 % HC1)-ACN]; gradient: 5%-40% B over 10.0 min). (2-hydroxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 30) was obtained.

[00535] 'H NMR (400 MHz, DMSO-d6): 5 = 10.30 - 9.99 (m, 1H), 7.73 (d, J = 7.8 Hz, 1H), 7.55 - 7.45 (m, 2H), 3.52 (s, 2H), 2.92 - 2.88 (m, 4H). Compound 31 Synthesis of (3-hydroxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid (Compound 31) O HO / ^x^B(OH)2 Synthesis of 4-bromo-2-methoxybenzimidamide

[00536] Scheme 31A LiHMDS (2.5 eq) THF, 0-25°C, 16 hrs 1                                                          2

[00537] To a solution of 4-bromo-2-methoxybenzonitrile (1) (3.00 g, 14.2 mmol, 1.00 eq) in THF (30 mL), was added LiHMDS (1 M, 35.37 mL, 2.50 eq) dropwise at 0°C and then stirred at 25°C for 16 h. LC-MS showed that (1) was consumed. The reaction mixture was quenched by addition of 4 M HCl / dioxane (40 mL). The combined organic layers were concentrated under reduced pressure to give a residue which was suspended in MeOH (30 mL) and stirred at 15°C for 2h, then filtered. The filtrate was concentrated under reduced pressure to give 4-bromo-2-methoxybenzimidamide (2) (3.50 g, crude) was obtained. The crude product was used in the next step without further purification. 'H NMR (400 MHz, DMSO-d6): 5 = 7.65 - 7.55 (m, 1H), 7.52 - 7.44 (m, 1H), 7.38 - 7.32 (m, 1H), 3.56 (s, 3H). Synthesis of 2-(4-bromo-2-methoxyphenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00539] To a solution of 4-bromo-2-methoxybenzimidamide (2) (3.50 g, 15.3 mmol, 1.00 eq) in MeOH (35 mL) was added K2CO3 (6.34 g, 45.8 mmol, 3.00 eq) and methyl 4-oxotetrahydrothiopyran-3-carboxylate (2.79 g, 16.0 mmol, 1.05 eq) at 25°C and then stirred at 60°C for 16 h. LC-MS showed (2) was consumed. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to give a residue that was purified by re-crystallization from MTBE (30 mL) at 25°C. 2-(4-bromo-2-methoxyphenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (3.00 g, crude) was obtained. 'H NMR (400 MHz, DMSO-d6): 5 = 7.24 (d, J = 8.1Hz, lH),7.16(s, 1H),7.11 -7.07 (m, 1H), 3.72 (s, 3H), 3.45 (s, 2H), 2.74 - 2.58 (m, 4H). Synthesis of 2-(2-methoxy-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one

[00540] Scheme 3IC o 3                                                              4

[00541] To a solution of 2-(4-bromo-2-methoxyphenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (3) (3.00 g, 8.49 mmol, 1.00 eq) in dioxane (30 mL) was added Pin2B2 (4.31 g, 17.0 mmol, 2.00 eq), K2CO3 (3.29 g, 23.8 mmol, 2.80 eq), and Pd(dppf)C12 (621 mg, 849 pmol, 0.10 eq) at 20°C under N2. The suspension was degassed and purged three times with N2. The mixture was stirred under N2 at 100°C for 16 h. LC-MS showed that (3) was consumed. The reaction mixture was concentrated under reduced pressure to remove solvent and then diluted with H2O (30 mL) and extracted with ethyl acetate (20 mL x 3). The combined organic layers were washed with brine (30 mL), dried over Na2SO4, filtered, and concentrated under reduced pressure to give a residue. The crude product was triturated with MTBE at 25°C for 120 min. 2-(2-methoxy-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (4) (2.5 g, crude) was obtained. The crude product was used in the next step without further purification. LC-MS: 401.2 (M+l). Synthesis of (3-methoxy-4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid 0 B(OH)2

[00543] ...

Claims

1. A pharmaceutical composition, comprising:a Wnt inhibitor which is 2-(4-(trifluoromethyl)phenyl)-l,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound A) preferably in an amount from about 0.01 wt% to about 2 wt%;a graphene oxide-hyaluronic acid conjugate (GO-HA), preferably wherein the GO is linked to the HA via -CH2-C(O)-NH-NH-C(O)-(CH2)4-C(O)NH-NH- where the HA is attached via an amide bond comprising the terminal NH of the linker and the GO is attached to the CH2 end of the linker (in some embodiments through an ether bond with GO phenol), and preferably in an amount from about 0.01 wt% to about 2 wt%;a glycol, preferably in an amount from about 2 wt% to about 10 wt%, or in an amount from about 3 wt% to about 6 wt%;one or more solubilizers, preferably in an amount from about 0.5 wt% to about 10 wt%, preferably about 1 wt% to about 6 wt%;one or more optional thickeners, and when present, preferably in an amount from about 0.5 wt% to about 2.5 wt%;one or more phospholipids, preferably in an amount from about 0.1 wt% to about 4 wt%;one or more carriers, preferably in an amount from about 2 wt% to about 10wt%, preferably about 3 wt% to about 6 wt% when the carrier is ethanol;an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%;an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%;an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; andsaline or water; andwherein the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more optional thickeners (when present), one or more phospholipids, one or more carriers, optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and saline or water is 100 wt%; or wherein the wt% of the WNT inhibitor, GO-HA, glycol, one or more solubilizers, one or more phospholipids, one or more carriers, optional stabilizer, (when present) optionalbuffer (when present), optional preservative (when present), and saline or water is 100 wt%;wherein the wt% ratio of the WNT inhibitor to GO-HA is about 0.1:1 to about 12:1.

2. The pharmaceutical composition of claim 1, wherein at least one of the stabilizer, buffer, and preservative is present; or wherein each of the stabilizer, buffer, and preservative is present.

3. A pharmaceutical composition, comprising:a WNT inhibitor of Formula (I), preferably in an amount from about 0.01 wt% to about 2 wt%;an optional graphene oxide-hyaluronic acid conjugate (GO-HA), and when present, preferably in an amount from about 0.01 wt% to about 2 wt%, and preferably wherein the GO is linked to the HA via -CH2-C(O)-NH-NH-C(O)-(CH2)4-C(O)NH-NH- where the HA is attached via an amide bond comprising the terminal NH of the linker and the GO is attached to the CH2 end of the linker (in some embodiments through an ether bond with GO phenol);a glycol, preferably in an amount from about 2 wt% to about 10 wt%, preferably about 3 wt% to about 6 wt%;one or more solubilizers, preferably in an amount from about 0.5 wt% to about 10 wt%, preferably about 1 wt% to about 6 wt%;one or more optional thickeners, and when present, preferably in an amount from about 0.5 wt% to about 2.5 wt%;one or more optional phospholipids, and when present, preferably in an amount from about 0.1 wt% to about 4 wt%;one or more optional carriers, and when present, preferably in an amount from about 2 wt% to about 10 wt% or in an amount from about 3 wt% to about 6 wt% when the carrier is ethanol;an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%;an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%;an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; andsaline or water; andwherein the wt% of the WNT inhibitor, optional GO-HA (when present), glycol, one or more solubilizers, one or more optional thickeners (when present), one or more optional phospholipids (when present), one or more optional carriers (when present), optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and water or saline is 100 wt%; or wherein the wt% of the WNT inhibitor, optional GO-HA (when present), glycol, one or more solubilizers, one or more optional phospholipids (when present), one or more optional carriers (when present), optional stabilizer (when present), optional buffer (when present), optional preservative (when present), and water or saline is 100 wt%;wherein the wt% ratio of the WNT inhibitor to GO-HA, when present, is about 0.1:1 to about 12:1; andwherein the WNT inhibitor of Formula (I) is according to:N R2R1 Ri’(I), or a single stereoisomer or mixture of stereoisomers thereof; a single tautomer or mixture of tautomers thereof; and / or a pharmaceutically acceptable salt thereof;wherein:R1 is hydrogen, deuterium, Ci-Csalkyl, -OH, -O-Ci-Csalkyl, -CH2OH, or -B(OH)2;Rla is hydrogen, deuterium, or Ci-Csalkyl;R2is(a) phenyl substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups;(b) phenyl substituted with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups and where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups;(c) phenyl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl-R3 where the phenyl in -phenyl-R3 is optionally substituted with 1, 2, or 3 R3a groups;(d) 5- or 6-membered heteroaryl substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups;(e) 5- or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -phenyl-R3 where the phenyl is additionally optionally substituted with 1, 2, or 3 R3a groups;(f) 5- or 6-membered heteroaryl optionally substituted with 1, 2, or 3 R3a groups and additionally substituted with -(5- or 6-membered heteroaryl)-R3 where the 5-or 6-membered heteroaryl in -(5- or 6-membered heteroaryl)-R3 is optionally substituted with 1, 2, or 3 R3a groups;(g) Cs-Cecycloalkyl substituted with R3 and additionally optionally substituted with 1 or 2 R3a groups;(h) Cs-Cecycloalkyl substituted with 5- or 6-membered heteroaryl where the 5- or 6-membered heteroaryl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups, and where the Cs-Cecycloalkyl is optionally substituted with 1 or 2 R3a groups;(j) Cs-Cecycloalkyl substituted with phenyl where the phenyl is substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups, and where the Cs-Cecycloalkyl is optionally substituted with 1 or 2 R3a groups;(k) 3- to 8-membered heterocycloalkyl substituted with phenyl or substituted with 5- or 6-membered heteroaryl, where the phenyl and the 5- or 6-membered heteroaryl are substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups; or(m) -CH=CH-R5 where R5 is phenyl or 5- or 6- membered heteroaryl, where the phenyl and the 5- or 6-membered heteroaryl are substituted with R3 and additionally optionally substituted with 1, 2, or 3 R3a groups;R3 is independently selected from -B(0H)2, cyano, halo, halo-Ci-Cealkyl, -(Co-C6alkylene)-0-R4, or 5- to 10-membered heterocyclic wherein the 5- to 10-membered heterocyclic is optionally substituted with cyano; or when R2 is (a), then R3 and one R3a, when on adjacent carbons, together with the carbons to which they are attached form:OH i(a-1),where the * indicate the carbons shared with a phenyl ring and where the remaining optional R3a on the phenyl ring portion are as defined below, and each R7a is independently hydrogen or Ci-Cealkyl;each R3a is independently selected from cyano, halo, -OH, Ci-Cealkyl, halo-Ci-Cealkyl, and Ci-Cealkoxy; andR4 is hydroxy-Ci-Cealkyl, Ci-Cealkoxy-Ci-Cealkyl, or Ci-Cealkoxycarbonyl-NH-Ci-Cealkyl.

4. The pharmaceutical composition of claim 3, wherein the WNT inhibitor is selected fromor a single stereoisomer or mixture of stereoisomers thereof; a single tautomer or mixture of tautomers thereof; and / or a pharmaceutically acceptable salt thereof.

5. The pharmaceutical composition of claim 3 or 4, wherein the WNT inhibitor is (4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)boronic acid(Compound 1); a single tautomer or mixture of tautomers thereof and / or a pharmaceutically acceptable salt thereof.

6. The pharmaceutical composition of claim 3 or 4, wherein the WNT inhibitor is 2-(4-(6-bromopyridin-3-yl) phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 7); a single tautomer or mixture of tautomers thereof and / or a pharmaceutically acceptable salt thereof.

7. The pharmaceutical composition of claim 3 or 4, wherein the WNT inhibitor is 2-(4-(2-(2-hydroxyethoxy)propan-2-yl)phenyl)-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-4-one (Compound 8); a single tautomer or mixture of tautomers thereof and / or a pharmaceutically acceptable salt thereof.

8. The pharmaceutical composition of claim 3 or 4, wherein the WNT inhibitor is (4'-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)-[l,T-biphenyl]-4-yl)boronic acid (Compound 10); a single tautomer or mixture of tautomers thereof and / or a pharmaceutically acceptable salt thereof.

9. The pharmaceutical composition of claim 3 or 4, wherein the WNT inhibitor is methyl (2-((2-(4-(4-oxo-3,5,7,8-tetrahydro-4H-thiopyrano[4,3-d]pyrimidin-2-yl)phenyl)propan-2-yl)oxy)ethyl)carbamate (Compound 18); a single tautomer or mixture of tautomers thereof and / or a pharmaceutically acceptable salt thereof.

10. The pharmaceutical composition of any one of claims 1 to 9, comprising: the WNT inhibitor in an amount from about 0.01 wt% to about 2 wt%;optional GO-HA, and when present, in an amount from about 0.01 wt% to about 2 wt%;a glycol in an amount from about 2 wt% to about 10 wt%;one or more solubilizers in an amount from about 2 wt% to about 10 wt%;one or more thickeners in an amount from about 0.5 wt% to about 2.5 wt%;a phospholipid in an amount from about 0.1 wt% to about 4 wt%;one or more carriers in an amount from about 2 wt% to about 10 wt%;an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%;an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%;an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; andsaline or water; andwherein the GO-HA is not optional when the WNT inhibitor is Compound A; and wherein one or more embodiments, the stabilizer, buffer, and preservative are not present.

11. The pharmaceutical composition of any one of claims 1 to 10, wherein a stabilizer is present.

12. The pharmaceutical composition of claim 11, wherein the stabilizer is an antioxidant.

13. The pharmaceutical composition of claim 11 or 12, wherein the stabilizer is methionine.

14. The pharmaceutical composition of any one of claims 1 to 13, wherein a buffer is present.

15. The pharmaceutical composition of any one of claims 1 to 14, wherein the pH is in a range from about 5.5 to about 7.

16. The pharmaceutical composition of claim 14 or 15, wherein the buffer is or comprises citric acid.

17. The pharmaceutical composition of any one of claims 1 to 16, wherein a preservative is present.

18. The pharmaceutical composition of any one of claims 1 to 17, wherein the preservative is present and comprises phenoxyethanol and ethylhexylglycerin, or is potassium sorbate.

19. The pharmaceutical composition of any one of claims 1 to 18, wherein the glycol is one or more selected from the group consisting of butylene glycol and propanediol (including propylene glycol).

20. The pharmaceutical composition of any one of claims 1 to 19, wherein the one or more solubilizers comprise PPG-26 Buteth-26 and PEG-40 hydrogenated castor oil; or the one or more solubilizers are one or more selected from the group consisting of hydroxypropyl cellulose, polysorbate 20, polysorbate 40, polysorbate 60, and polysorbate 80.

21. The pharmaceutical composition of any one of claims 3 to 9, and 11 to 20, wherein a phospholipid is present.

22. The pharmaceutical composition of any one of claims 1 to 21, wherein the phospholipid is phosphatidylcholine.

23. The pharmaceutical composition of any one of claims 1 to 22, wherein one or more carriers is present.

24. The pharmaceutical composition of claim 23, wherein the one or more carriers comprises or is ethanol.

25. The pharmaceutical composition of any one of claims 1 to 24, wherein the saline is from about 0.22% to about 0.9% NaCl in water.

26. The pharmaceutical composition of any one of claims 1 to 25, wherein the pharmaceutical composition comprises the WNT inhibitor, a glycol, one or two solubilizers, one or two thickeners, a phospholipid, ethanol, and water or saline; and optionally contains GO-HA; wherein the GO-HA is not optional when the WNT inhibitor is Compound A; and wherein the ratio of WNT inhibitor:GO-HA, when GO-HA is present, is about 1:1.

27. The pharmaceutical composition of any one of claims 1, 2, and 10 to 26 wherein the wt% ratio of WNT inhibitor:phospholipid is from about 1:1 to about 1:50, or about 1:10 to about 1:25, or about 1:10 to about 1:13.

28. The pharmaceutical composition of any one of claims 3 to 27, wherein GO-HA is present.

29. The pharmaceutical composition of any one of claims 1, 2, and 10 to 28, wherein the wt% ratio of WNT inhibitor:GO-HA is from about 1:0.1 to about 1:1.

30. The pharmaceutical composition of any one of claims 1 to 29, wherein one or more carriers and phospholipid are present and are in a wt% ratio of carrier(s):phospholipid is from about 2:1 to about 4:1.

31. The pharmaceutical composition of any one of claims 1 to 30, glycol and one or more solubilizer(s) are present in a wt% ratio of about 1:0.5 to about 1:1.5.

32. The pharmaceutical composition of any one of claims 1 to 31, wherein glycol and one or more carrier(s) are present in a wt% ratio of about 1:0.5 to about 1:1.5, wherein one or more embodiments, the one or more carrier is ethanol.

33. The pharmaceutical composition of any one of claims 3 to 9, 19 to 25, and 27 to 32, comprising:the WNT inhibitor in an amount from about 0.01 wt% to about 2 wt%;optional GO-HA, and when present, in an amount from about 0.01 wt% to about 2 wt%;a glycol in an amount from about 2 wt% to about 10 wt%;one or more thickeners in an amount from about 0.5 wt% to about 2.5 wt%;an optional stabilizer, and when present, preferably in an amount from about 0.1 wt% to about 0.5 wt%;an optional buffer, and when present, preferably in an amount from about 0.2 wt% to about 2.0 wt%;an optional preservative, and when present, preferably in an amount from about 0.025 wt% to about 1.5 wt%; andsaline or water; andwherein the GO-HA is not optional when the WNT inhibitor is Compound A;wherein the one or more optional phospholipids and the one or more carriers are not present; andwherein one or more embodiments, the stabilizer, buffer, and preservative are not present.

34. The pharmaceutical composition of any one of claims 1 to 33, wherein the pharmaceutical composition comprises the WNT inhibitor, a glycol, one or two thickeners, and water or saline; and optionally contains GO-HA; and wherein the ratio of WNT inhibitor:GO-HA, when GO-HA is present, is about 1:1.

35. The pharmaceutical composition of claim 33 or 34, wherein glycol and one or more thickener(s) are present in a wt% ratio of about 1:0.25 to about 1:1.5.

36. The pharmaceutical composition of any one of claims 1-9, 11 to 25, and 27-32, wherein the pharmaceutical composition does not comprise a thickener.

37. The pharmaceutical composition of any one of claims 1 to 35, wherein the pharmaceutical composition comprises a thickener.

38. The pharmaceutical composition of any one of claims 1 to 37, wherein the pharmaceutical composition does not comprise a thickener or comprises a thickener in an amount suitable for a spray.

39. The pharmaceutical composition of any one of claims 1 to 35 and 37, wherein the pharmaceutical composition comprises a thickener and the pharmaceutical composition is a serum.

40. The pharmaceutical composition of any one of claims 1 to 39, wherein when the GO-HA is present, the GO is linked to the HA via -CH2-C(O)-NH-NH-C(O)-(CH2)4-C(O)NH-NH-where the HA is attached via an amide bond comprising the terminal NH of the linker and the GO is attached to the CH2 end of the linker (in some embodiments through an ether bond with GO phenol).

41. A method of treating a disease, disorder, or condition associated with Wnt signaling pathway activity, comprising administering topically a pharmaceutical composition of any one of claims 1 to 40 to a mammal in need thereof.

42. The method of claim 41, wherein the method is for stimulating regeneration of tissue at a wound in the mammal in need thereof and wherein the wound is contacted with a therapeutically effective amount of the pharmaceutical composition.

43. The method of claim 41, wherein the disease, disorder, or condition is selected from a chronic wound, an acute wound, an alkali-burned corneal wound, an incisional wound (open or closed), a bum, a lesion (including lesions caused by HPV and / or a vims selected from the Poxviridae family of viruses), an inflammatory dermatitis disease (including acne, psoriasis, rosacea, and scleroderma), cancer (including melanoma and breast cancer), a denerved body part in need of reinnervation, tissue in need of regeneration (including damaged elastic cartilage), bacterial growth in need of inhibition, fungal growth in need of inhibition, and tissue in need of neovascularization.

44. The method of claim 41, wherein the method is for inducing bacteriostasis associated with Wnt signaling pathway activity.

45. The method of claim 41, wherein the method is for treating an open excisional wound, and the pharmaceutical composition is according to any one of claims 1 to 35.

46. The method of claim 45, wherein the WNT inhibitor is Compound A.

47. The method of claim 41, wherein the method is for treating a closed excisional wound the pharmaceutical composition is according to any one of claims 1 to 25 and 33 to 37.

48. The method of claim 41, wherein the method is for treating a bum and the pharmaceutical composition is according to any one of claims 1 to 25 and 33 to 37.

49. The method of claim 41 or 42, wherein the method is for regenerating elastic cartilage and the pharmaceutical composition is according to any one of claims 1 to 37.

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