Methods and compositions for preventing hermaphroditism in cannabis plants and for making male sterile cannabis plants

Abstract

This document describes methods and compositions for preventing hermaphroditism in Cannabis plants, as well as methods and compositions for making male sterile Cannabis plants.

Description

In one embodiment, the composition is an aqueous solution. In one embodiment, the composition is formulated as a spray. In one embodiment, the composition is dried / lyophilized. In one embodiment, the RNA nucleic acid molecules are double-stranded (ds) RNA nucleic acid molecules. In one embodiment, the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs:33-43, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the AMS gene. In one embodiment, the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs:44-54, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the bHLH91 gene. In one embodiment, the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs: 55-65, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the at TM6 gene. In one embodiment, the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs :66-80, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the STP6 gene. In one embodiment, the composition further comprises a delivery vehicle. In one embodiment, the delivery vehicle is a nanoparticle. In another aspect, articles of manufacture are provided. Such articles of manufacture typically include RNA nucleic acid molecules, wherein the RNA nucleic acid molecules are at least 10 nucleotides in length and have at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to at least one of the genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80, wherein the RNA nucleic acid molecules effectively silence expression of the at least one gene, wherein the AMS gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLH91 gene comprises any of SEQ ID NOs: 2 or 89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYT1 gene comprises any of SEQ ID NOs: 5 or 109-115; wherein theMYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein theMYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124; wherein the MYB80 gene comprises any of SEQ ID NOs: 8 or 125-127; and a delivery vehicle. In one embodiment, the delivery vehicle is a nanoparticle. In one embodiment, the nanoparticle is selected from chitosan salts (e.g., CQAS), carbon dots, liposomes, clay nanosheets, and gold nanoparticles. In one embodiment, the delivery vehicle is conjugated to the RNA nucleic acid molecules. In one embodiment, the delivery vehicle is not conjugated to the RNA nucleic acid molecules. In one embodiment, the articles of manufacture further include a linker. In one embodiment, the article of manufacture is formulated as a spray. In still another aspect, methods of preventing hermaphroditism (e.g., in females) or the viability in male flowers (e.g., in male hermaphrodites or biological males) in Cannabis plants is provided. Such methods typically include contacting Cannabis plants with any of the compositions described herein. In one embodiment, the contacting is spraying. In one embodiment, the contacting is performed for about 10 seconds to about 60 seconds (per plant) at least one time a day. In one embodiment, the contacting is performed for about 10 seconds to about 60 seconds (per plant) a plurality of times each day. In yet another aspect, methods of producing male sterile Cannabis plants (e.g., hermaphroditic female or a biological male) are provided. Such methods typically include introducing a nucleic acid molecule into Cannabis cells to produce genetically-engineered Cannabis cells, wherein the nucleic acid molecule comprises a sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to one or more genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80 under direction of a promoter sequence, wherein the AMS gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLH91 gene comprises any of SEQ ID NOs: 2 or 89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYT1 gene comprises any of SEQ ID NOs: 5 or 109-115; wherein theMYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein theMYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124; wherein the MYB80 gene comprises any of SEQ ID NOs: 8 or 125-127; and regenerating a genetically engineered Cannabis plant from the genetically engineered Cannabis cells, wherein the genetically engineered Cannabis plant exhibits reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein. In one embodiment, expression of the nucleic acid molecule triggers RNAi, thereby silencing expression of the at least one gene. In one embodiment, pollen produced by the genetically engineered Cannabis plants is non-viable. In one embodiment, the methods further include selecting for genetically engineered Cannabis plants. In one aspect, methods of producing male sterile Cannabis plants (e.g., hermaphroditic female or a biological male) is provided. Such methods typically include introducing a nucleic acid molecule into Cannabis cells under conditions in which the genome of the Cannabis cells is edited to produce edited Cannabis cells, wherein the nucleic acid molecule has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to one or more genes selected from AMS, bHLH91, TM6, STP6, DYTl, MYB35, MYB35, and MYB80, wherein the AMS gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLH91 gene comprises any of SEQ ID NOs: 2 or 89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYTl gene comprises any of SEQ ID NOs: 5 or 109-115; wherein the MYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein the MYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124; wherein the MYB80 gene comprises any of SEQ ID NOs: 8 or 125-127; and regenerating an edited Cannabis plant from the edited Cannabis cells, wherein the edited Cannabis plant exhibits reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein. In one embodiment, the nucleic acid molecule comprises one or more constructs comprising gene editing components for editing at least one of the genes selected from AMS, bHLH91, 1M6, STP6, DYTl, MYB35, MYB35, and MYB80, wherein the gene editing reduces or knocks-out expression from the one or more genes or the expressed protein exhibits reduced, altered, or lack of function. In one embodiment, the gene editing components are selected from CRISPR / Cas components, TALEN components, or the like. In one embodiment, the pollen produced by the edited Cannabis plants is non-viable. In still another aspect, methods of making a Cannabis plant (e.g., hermaphroditic female or a biological male) male sterile are provided. Such methods typically include contacting Cannabis seeds with a mutagen to create mutant Cannabis seeds; producing mutant Cannabis plants from the mutant Cannabis seeds; and screening the mutant Cannabis plants for those having a mutation in one or more genes selected from AMS, bHLH91, TM6, STP6, DYTl, MYB35, MYB35, and MYB80, wherein the mutant Cannabis plants exhibit reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein. In still another aspect, methods of evaluating a Cannabis plant for the propensity to trigger male flower formation (e.g., hermaphroditism) are provided. Such methods typically include determining the presence or absence of a variation in sequence within one or more genes selected from AMS, bHLH91, 1M6, STP6, DYTl, MYB35, MYB35, and MYB80, wherein the presence or absence of sequence variation within one or more of the genes is indicative of the propensity to trigger male flower formation (e.g., hermaphroditism) in the Cannabis plant. In another aspect, methods of obtaining a male sterile or hermaphroditic-resistant Cannabis plant are provided. Such methods typically include a) crossing a first Cannabis plant and a second Cannabis plant, wherein the first Cannabis plant exhibits male sterility or hermaphroditic-resistance; b) hybridizing genomic DNAfrom cells obtained from the cross or from cells of later filial generations of the cross with one or more markers within one or more genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80 and; c) selecting a plant in which the genomic DNA hybridizes with one or more of the markers within one or more of the genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80 to obtain a male sterile or hermaphroditic-resistant plant. In one aspect, compositions are provided that include an RNA nucleic acid molecule, where the RNA nucleic acid molecule has at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to at least one of the genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), andMYB80 (SEQ ID NO: 8), where the RNA nucleic acid molecule effectively silences expression of the at least one gene. In some embodiments, the composition is an aqueous solution. In some embodiments, the composition is formulated as a spray. In some embodiments, the composition is dried / lyophilized. In some embodiments, the RNA is a double-stranded (ds) RNA. In some embodiments, the composition comprises RNA nucleic acid molecules having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to at least two of the genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), andMYB80 (SEQ ID NO:8), wherein the RNA nucleic acid molecules effectively silence expression of the at least two genes. In some embodiments, the RNA nucleic acid molecules having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to the AMS gene are selected from any of SEQ ID NOs:33-43, wherein each of the RNA nucleic acid molecules effectively silence expression of the AMS gene. In some embodiments, the RNA nucleic acid molecules having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to the bHLH91 gene are selected from any of SEQ ID NOs :44-54, wherein each of the RNA nucleic acid molecules effectively silence expression of the bHLH91 gene. In some embodiments, the RNA nucleic acid molecules having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to the TM6 gene are selected from any of SEQ ID NOs:55-65, wherein each of the RNA nucleic acid molecules effectively silence expression of the at TM6 gene. In some embodiments, the RNA nucleic acid molecules having at least 80% sequence identity (eg., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to the STP6 gene are selected from any of SEQ ID NOs:66-80, wherein each of the RNA nucleic acid molecules effectively silence expression of the STP6 gene. In some embodiments, the composition further comprises a delivery vehicle. In some embodiments, the delivery vehicle is a nanoparticle. In another aspect, articles of manufacture are provided that include RNA nucleic acid molecules, wherein the RNA nucleic acid molecules have at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to at least one of the genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), and MYB80 (SEQ ID NO:8), wherein the RNA nucleic acid molecules effectively silence expression of the at least one gene; and a delivery vehicle. In some embodiments, the delivery vehicle is a nanoparticle. Representative nanoparticles include, without limitation, chitosan salts (e.g., CQAS), carbon dots, liposomes, clay nanosheets, and gold nanoparticles. In some embodiments, the delivery vehicle is conjugated to the RNA nucleic acid molecules. In some embodiments, the delivery vehicle is not conjugated to the RNA nucleic acid molecules. In some embodiments, the article of manufacture further includes a linker. In some embodiments, the article of manufacture is formulated as a spray. In yet another aspect, methods of preventing hermaphroditism (e.g., in females) or the viability in male flowers (e.g., in male hermaphrodites or biological males) in Cannabis plants is provided. Such methods typically include contacting Cannabis plants with a composition as described herein. In some embodiments, the contacting is spraying. In some embodiments, the contacting is performed for about 10 seconds to about 60 seconds (per plant) at least one time a day. In some embodiments, the contacting is performed for about 10 seconds to about 60 seconds (per plant) a plurality of times each day. In still another aspect, methods of producing male sterile Cannabis plants (e.g., hermaphroditic female or a biological male) are provided. Such methods typically include introducing a nucleic acid molecule into Cannabis cells to produce genetically-engineered Cannabis cells, wherein the nucleic acid molecule comprises a sequence having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to one or more genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NON), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), and MYB80 (SEQ ID NO:8) under direction of a promoter sequence; and regenerating a genetically engineered Cannabis plant from the genetically engineered Cannabis cells, where the genetically engineered Cannabis plant exhibits reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein. In some embodiments, expression of the nucleic acid molecule triggers RNAi, thereby silencing expression of the at least one gene. In some embodiments, pollen produced by the genetically engineered Cannabis plants is non-viable. In some embodiments, the methos further include selecting for genetically engineered Cannabis plants. In another aspect, methods of producing male sterile Cannabis plants (e.g., hermaphroditic female or a biological male) are provided. Such methods typically include introducing a nucleic acid molecule into Cannabis cells under conditions in which the genome of the Cannabis cells is edited to produce edited Cannabis cells, wherein the nucleic acid molecule has at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to one or more genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NON), STP6 (SEQ ID NON), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), and MYB80 (SEQ ID N0:8); and regenerating an edited Cannabis plant from the edited Cannabis cells, where the edited Cannabis plant exhibits reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein. In some embodiments, the nucleic acid molecule comprises one or more constructs comprising gene editing components for editing at least one of the genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), andMYB80 (SEQ ID NO:8), wherein the gene editing reduces or knocks-out expression from the one or more genes or the expressed protein exhibits reduced, altered, or lack of function. In some embodiments, the gene editing components are selected from CRISPR / Cas components, TALEN components, or the like. In some embodiments, the pollen produced by the edited Cannabis plants is non-viable. In yet another aspect, methods of making a Cannabis plant (e.g., hermaphroditic female or a biological male) male sterile are provided. Such methods typically include contacting Cannabis seeds with a mutagen to create mutant Cannabis seeds; producing mutant Cannabis plants from the mutant Cannabis seeds; and screening the mutant Cannabis plants for those having a mutation in one or more genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), and MYB80 (SEQ ID NO:8), where the mutant Cannabis plants exhibit reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein. In still another aspect, methods of evaluating a Cannabis plant for the propensity to trigger male flower formation (e.g., hermaphroditism) are provided. Such methods typically include determining the presence or absence of a variation in sequence (e.g., SNP, insertion, deletion, etc.) within one or more genes selected from AMS (SEQ ID NO: 1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35 (SEQ ID NO:6), MYB35 (SEQ ID NO:7), and MYB80 (SEQ ID NO:8), where the presence or absence in sequence variation within one or more of the genes is indicative of the propensity to trigger male flower formation (e.g., hermaphroditism) in the Cannabis plant. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the methods and compositions of matter belong. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the methods and compositions of matter, suitable methods and materials are described below. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. DESCRIPTION OF DRAWINGS FIGs. 1A-1D are schematics showing the dsRNA constructs for AMS (1A), bHLH91 (IB), TM6 (IC), and STP6 (ID). FIG. 2 are schematics showing the experimental design for each cocktail. FIG. 3 is an image of stained viable pollen from the Control Treatment group in the Cocktail 1 Experimental. The image shows almost 100% viable pollen from an ‘Original Bacio’ plant after being sprayed with 0.02% CQAS solution with no dsRNA (i.e., control). This pollen stain slide shows the male pollen produced by the female plant after STS application were fully viable as non-shriveled pollen grains with dark contents inside the grain indicate complete pollen viability (i.e., non-aborted). Pollen grains that look fully or largely empty without dark contents inside are not viable (i.e., aborted pollen). FIG. 4 is an image of stained pollen from the Cocktail 1 Experimental Treatment group. The image shows nearly 100% non-viable pollen from ‘Original Bacio’ plant after application of the dsRNA—0.02% CQAS solution. Effect of dsRNA Cocktail 1 on an ‘Original Bacio’ plant resulted in shriveled pollen grains without dark contents inside the grain, which indicates complete pollen inviability (i.e., aborted pollen). This image shows almost complete pollen abortion after four weeks of biweekly spraying. The pollen in this image was harvested three days after the final dsRNA spray. FIG. 5 is an image of stained pollen from the Cocktail 2 Experimental Design Grow Tent prior to dsRNA—0.02% CQAS solution application, representing the before application (i.e., control) conditions. Image is of 100% viable pollen from an ‘Original Bacio’ plant. Nonshriveled pollen grains with dark contents inside the grain indicate complete pollen viability (non-aborted). Pollen grains that look fully or largely empty without dark contents inside are not viable. This pollen stain slide shows the male pollen produced by the female plant after STS application but prior to Cocktail 2 dsRNA application were fully viable. FIG. 6 is an image of stained pollen from the Cocktail 2 Experimental Design Grow Tent after dsRNA—0.02% CQAS solution application. Effect of dsRNA—0.02% CQAS Cocktail 2 solution on the same ‘Original Bacio’ plant as FIG. 5 showcasing a typical plant response posttreatment. This pollen sample was taken six days after a single application of Cocktail 2 dsRNA—0.02% CQAS spray. Non-shriveled pollen grains with dark contents inside the grain indicate complete pollen viability (non-abortion), while pollen grains that look fully or largely empty without dark contents inside are not viable. The pollen grains in this image are all non-viable. FIG. 7 is a graph showing pollen abortion rates in the presence of Cocktail 1 between the Control Treatment Grow Tent and the Experimental Treatment Grow Tent. The plants in the Experimental Treatment Grow Tent received a spray containing bHLH91 dsRNA and AMS dsRNA. Pollen abortion significantly increased in the Experimental Tent compared to the Control Tent. *, P<0.05. FIG. 8 is a graph showing pollen abortion rates before and after application of Cocktail 2, which contains STP6 dsRNA and TM6 dsRNA. Pollen abortion significantly increased after spraying. *, P<0.05. FIG. 9 is a graph showing the effect of Cocktail 1 (bHLH91 and AMS dsRNA) on AMS gene expression. AMS showed significantly reduced gene expression when sprayed with AMS dsRNA relative to control plants. Gene expression values are log-transformed. Statistical significance of** indicates P<0.01. FIG. 10 is a graph showing the effect of Cocktail 1 (bHLH91 and AMS dsRNA) on bHLH91 gene expression. bHLH91 showed significantly reduced gene expression when sprayed with bHLH91 dsRNA relative to control plants. Gene expression values are log-transformed. Statistical significance of** indicates P<0.01. FIG. 11 is a graph showing the effect of Cocktail 2 (TM6 and STP6 dsRNA) on STP6 gene expression. STP6 showed significantly reduced gene expression after being sprayed with STP6 dsRNA relative to expression before being sprayed. Gene expression values are log-transformed and analysis was paired. Statistical significance of ** indicates P<0.01 FIG. 12 is a graph showing the effect of Cocktail 2 (TM6 and STP6 dsRNA) on TM6 gene expression. TM6 showed significantly reduced gene expression after being sprayed with TM6 dsRNA in ‘Original Bacio’ relative to expression before being sprayed. Gene expression values are log-transformed and analysis was paired. Statistical significance of * indicates P<0.05., n.s. means not statistically significant. FIG. 13-1 and 13-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for AMS. FIG. 14-1 and 14-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for bHLH9I. FIG. 15-1 and 15-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for TM6. FIG. 16-1 and 16-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for STP6. FIG. 17-1 and 17-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for DYT1. FIG. 18-1 and 18-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for MYB35-15. FIG. 19-1 and 19-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for MYB35-16. FIG. 20-1 and 20-2 is a schematic pile-up showing the conserved and variable regions within the 8 common genotypes for MYB80. DETAILED DESCRIPTION Plant male sterility refers to the failure of a plant to produce dehiscent anthers, functional pollen, and viable male gametes. In this disclosure, we identify key male reproductive genes in the genus Cannabis and demonstrate how silencing these genes creates male sterility by inhibiting functional pollen formation and the formation of viable male gametes in a Cannabis plant. Additionally, this document describes an anti-hermaphroditism spray for Cannabis plant cultivation and breeding management, and establishes methods to create male sterile, hermaphroditic resistant, and, or hermaphroditical stress-resistant Cannabis plants, plant lines or cultivars through the inactivation of these genes. Further, through the manipulation of these genes, this document describes a male-sterility-fertility restoration system in Cannabis, and also the use of one or more of these genes in marker-assisted breeding programs. Genes Involved in Male Flower Production Using methods described in this disclosure, eight genes were identified as having a role in the hermaphrodite phenotype and male reproductive development in Cannabis. The sequence of each gene from cslO are provided as follows: AMS (SEQ ID NO:1), bHLH91 (SEQ ID NO:2), TM6 (SEQ ID NO:3), STP6 (SEQ ID NO:4), DYT1 (SEQ ID NO:5), MYB35-15 (SEQ ID NO:6), MYB35-16 (SEQ ID NO:7) and A / IW (SEQ ID NO:8) (see Table 1). The eight genes are predicted to encode the proteins having the amino acid sequences shown in SEQ ID NO:9 (AMS), SEQ ID NO: 10 (bHLH91), SEQ ID NO: 11 (TM6), SEQ ID NO: 12 (STP6), SEQ ID NO:13 and 14 (DYT1), SEQ IDN0:15 (MYB35-15), SEQ IDN0:16 (MYB35-16), and SEQ ID NO: 17 (MYB80). Brief summaries of each are provided below, with a focus on homologs in other plant species (i.e., orthologs) and / or paralogs, since, prior to this disclosure, these genes have not been identified or characterized in Cannabis. Gene is used herein to refer to the nucleic acid sequences that, upon expression, produce a protein (i.e., the coding sequence), and also to non-expressed nucleic acid sequences (e.g., introns, 3’ and / or 5’ non-translated regions, etc.) and nucleic acid sequences that act as regulatory regions (e.g., promoters, enhancers, etc.). It would be appreciated that regulatory regions can act on a coding sequence from afar (e.g., up to 5 kb upstream and / or downstream of a coding sequence; see, for example, Chen et al., 2020, Nat. Commun., 11:2472). Each of the genes identified as being involved in male flower production was evaluated across almost 200 different publicly-available Cannabis accessions to examine the variation found within each gene in Cannabis. The gene sequence, including up to 5 kb upstream and downstream of the coding sequence for each individual were clustered into groups. A reference sequence representative of each cluster was chosen to represent the common genotype. Pairwise blast similarities were calculated between the reference and the other individuals in the cluster. Consensus sequences were identified for each gene that, based on sequence identity, represent the primary categories of variation. AMS (A borted Microspore) The ABORTED MICROSPORE (AMS) gene in Arabidopsis is a transcription factor involved in the gene cascade related to anther and microspore development. AMS is a regulator of pollen wall formation and sporopollenin biosynthesis and is a homolog to TDR1 (TAPETUM DEGENERATION RETARDATIONl) in rice. The AMS gene sequence from Cannabis is shown in SEQ ID NO: 1, and the putative encoded amino acid sequence is shown in SEQ ID NO:9. SEQ ID NOs: 81-88 represent the eight most common genotype sequences for AMS (see Table 2), and FIG. 13 is a schematic alignment of the eight most common genotype sequences for AMS, depicting conserved regions and variable regions. bHLH91 (Basic Helix-Loop-Helix 91) bHLH91 is a transcription factor involved in developing pollen and tapetum in Arabidopsis. bHLH91 is a homolog to bHLHIO, bHLH89, and bHLH90 in Arabidopsis and to EAT1 in rice. The fact that Arabidopsis has multiple homologs to the Cannabis bHLH91 indicates that sex determination in Cannabis is different than in species like Arabidopsis. The bHLH91 gene sequence from Cannabis is shown in SEQ ID NO:2, and the putative encoded amino acid sequence is shown in SEQ ID NO: 10. SEQ ID NOs: 89-92 represent the four most common genotype sequences for bHLH91 (see Table 2), and FIG. 14 is a schematic alignment of the four most common genotype sequences for bHLH91, depicting conserved and variable regions. TM6 (agamous-like MADS-box protein TM6) TOMATO MADS BOX GENE6 (TM6) is paralogous to APETALA3 (AP3) in Arabidopsis. Different plant species express one or the other for petal or stamen development, where TM6 informs stamen development. The TM6 gene sequence from Cannabis is shown in SEQ ID NO:3, and the putative encoded amino acid sequence is shown in SEQ ID NO: 11. SEQ ID NOs: 93-99 represent the seven most common genotype sequences for TM6 (see Table 2), and FIG. 15 is a schematic alignment of the seven most common genotype sequences for TM6, depicting the conserved and variable regions. STP6 (Sugar Transport Protein 6) Sugar Transport Protein6 (STP6) as well as other sugar transport proteins are mainly responsible for glucose uptake into the pollen grain in different plant species. In many species, STP6 is involved in the sugar supply of the germinating pollen or the growing pollen tube. The STP6 gene sequence from Cannabis is shown in SEQ ID NO:4, and the putative encoded amino acid sequence is shown in SEQ ID NO: 12. SEQ ID NOs: 100-108 represent the nine most common genotype sequences for STP6 (see Table 2), and FIG. 16 is a schematic alignment of the nine most common genotype sequences for STP6, depicting the conserved and variable regions. DYT1 (DYSFUNCTIONAL TAPETUM 1) Arabidopsis DYT 1 (DYSFUNCTIONAL TAPETUM 1) is homologous with UNDEVELOPED TAPETUM1 (UDT1) in rice and Male Sterility32 (Ms32) in maize and is involved in anther development and male fertility. The DYT1 gene sequence from Cannabis is shown in SEQ ID NO:5, and the putative encoded amino acid sequence includes two isoforms shown in SEQ ID NOs: 13 and 14. SEQ ID NOs: 109-115 represent the seven most common genotype sequences for DYT1 (see Table 2), and FIG. 17 is a schematic alignment of the seven most common genotype sequences for DYT1, depicting the conserved and variable regions. TDF1 (Defective in Meristem Development and Function 1) TDF1 (DEFECTIVE IN MERISTEM DEVELOPMENT AND FUNCTION 1) in Arabidopsis encodes a putative R2R3 MYB transcription factor that plays a role in tapetai differentiation and function and has homology to three different genes in Cannabis (2 MYB35 genes and 1 MYB80 gene). The fact that Cannabis has multiple homologs to the Arabidopsis TDF1 indicates that sex determination in Cannabis is more complex than in species like Arabidopsis. One of the MYB35 gene sequences from Cannabis (referred to herein as MYBISIS') is shown in SEQ ID NO:6, and the putative encoded amino acid sequence is shown in SEQ ID NO: 15; another MYB35 gene sequence from Cannabis (referred to herein asMYB35-16) is shown in SEQ ID NO:7, and the putative encoded amino acid sequence is shown in SEQ ID NO: 16; and theMYB80 gene sequence from Cannabis is shown in SEQ ID NO:8, and the putative encoded amino acid sequence is shown in SEQ ID NO: 17. SEQ ID NOs: 116-119 represent the four most common genotype sequences for MYB 3 515 (see Table 2), and FIG. 18 is a schematic alignment of the four most common genotype sequences for MYB35-15, depicting the conserved and variable regions. SEQ ID NOs: 120-124 represent the five most common genotype sequences for MYB35-16 (see Table 2), and FIG. 19 is a schematic alignment of the five most common genotype sequences for MYB35-16, depicting the conserved and variable regions. SEQ ID NOs: 125-127 represent the three most common genotype sequences for MYB80 5 (see Table 2), and FIG. 20 is a schematic alignment of the three most common genotype sequences for MYB80, depicting the conserved and variable regions. Table 1. Gene Information Gene Name Gene ID Chromosome Type (Autosomal, X, Y) Chromosome (NCBI Reference Sequence Identifier) Chromosomal location- start0 Chromosomal location- endd SEQ ID NO for nucleic acid SEQ ID NO for amino acid MIS LOC115706375 X Chromosome NC 044370.1 7644547 7657154 1 9 bHLH91 LOCI 15713 370 X Chromosome NC 044370.1 65758028 65770653 2 10 TM6 LOC115714657 Chromosome 4 NC 044373.1 85333862 85350865 3 11 STP6 LOC115714521 Chromosome 4 NC 044373.1 824664 837322 4 12 DYT1 LOCI 15704570 Chromosome 1 NC_044371.1 93242539 93253137 5 13, 14 MYB35-15 LOCI 15700367 Chromosome 8a NC 044379.1 60555650 60566877 6 15 MYB35-16 LOC115711316 Chromosome 3 NC_044372.1 83249697 83261426 7 16 MYB80 LOCI 15725196 Chromosome 6b NC_044377.1 372128 384260 8 17 a on Scaffold 9 in Sonr Pear Male, which corresponds to Chromosome X in cslO 10 b on Scaffold 4 in Sour Pear Male, which corresponds to Chromosome 8 in cslO c includes 5 kbp upstream of the transcription start site d includes 5 kbp downstream of tire transcription stop site 15               Table 2. Common Genotype Sequences Among Cannabis Accessions Gene Ref Seq (Genotype) Cluster # of Accessions a Accession # b SEQ ID NO AMSQ 1 1 13 122 81 2 2 13 88 82 3 3 38 136 83 4 4 65 118 84 5 6 22 176 85 6 9 9 3 86 7 11 19 87 87 8 12 9 120 88 bHLH91 d 1 2 121 1 89 2 4 32 3 90 3 8 15 146 91 4 9 10 157 92 TM6e 1 1 7 3 93 2 2 25 87 94 3 3 34 104 95 4 4 52 146 96 5 7 30 60 97 6 12 6 176 98 7 14 9 182 99 STP6i 1 2 22 36 100 2 3 25 134 101 3 4 18 122 102 4 5 46 3 103 5 6 13 173 104 6 8 18 60 105 7 9 21 88 106 8 15 5 1 107 9 16 6 87 108 DYT1 § 1 2 24 3 109 2 3 9 87 110 3 6 50 15 111 4 7 25 36 112 5 10 23 122 113 6 12 37 60 114 7 13 8 146 115 MYB35-1511 1 2 6 1 116 2 5 80 146 117 3 7 21 87 118 4 8 68 3 119 MYB35-16' 1 1 60 122 120 2 2 86 104 121 3 5 7 3 122 4 14 11 17 123 5 15 15 22 124 MYB8(P 1 1 4 122 125 2 2 108 3 126 3 3 65 60 127 a, the number of Accessions that fall within 95% sequence identity to the corresponding reference sequence, also referred to as a genotype sequence. b, see Table 8 for the Accession name corresponding to the indicated Accession number. e, see APPENDIX A for the sequences of the most common genotypes for AMS (i.e., reference sequences). d, see APPENDIX B for the sequences of the most common genotypes for bHLH91 (i.e., reference sequences). e, see APPENDIX C for the sequences of the most common genotypes for TM6 (i.e., reference sequences). see APPENDIX D for the sequences of the most common genotypes for STP6 (i.e., reference sequences). g, see APPENDIX E for the sequences of the most common genotypes for DYT1 (i.e., reference sequences). h, see APPENDIX F for the sequences of the most common genotypes for MYB35-15 (i.e., reference sequences). ', see APPENDIX G for the sequences of the most common genotypes for MYB35-16 (i.e., reference sequences). j, see APPENDIX H for the sequences of the most common genotypes for MYB80 (i.e., reference sequences). The starting and ending positions indicated in Table 1 above for each gene are relative to the cslO Cannabis genome, as are the gene structure schematics shown in FIG. 1A-1D. Intraspecific variation is seen in the chromosomal placement of these genes, depending on the genomic assembly of a specific Cannabis accession. For example, chromosome X in cslO corresponds to scaffold 9 in the Sour Pear genome in the LeafWorks genomic database and chromosome 8 in cslO corresponds to scaffold 4 in the Sour Pear genome. Also as described herein, there is variation within each gene across different Cannabis accession in introns as well as upstream and downstream regions, some characterized by large insertions. There are frequent small variations in gene start and stop locations and, occasionally, there is variation in the total number of exons. Internal gene regions are relatively conserved. See, for example, FIGs. 13-20. In addition to different chromosomal locations, it would be understood by a skilled artisan that the genes described herein (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35-15, MYB35-16, MYB80) can have a different sequence from those provided herein (e.g., the nucleic acids shown in SEQ ID NOs: 1-8 and the proteins shown in SEQ ID NOs: 9-16). For example, representative AMS sequences that differ from SEQ ID NO: 1 are shown in SEQ ID NOs: 81-88 and correspond to the eight most common genotype sequences for AMS in Cannabis; representative bHLH91 sequences that differ from SEQ ID NO:2 are shown in SEQ ID NOs: 8992 and correspond to the four most common genotype sequences for bHLH91 in Cannabis; representative TM6 sequences that differ from SEQ ID NO:3 are shown in SEQ ID NOs: 93-99 and correspond to the seven most common genotype sequences for TM6 in Cannabis; representative STP6 sequences that differ from SEQ ID NO:4 are shown in SEQ ID NOs: 100108 and correspond to the nine most common genotype sequences for STP6 in Cannabis; representative DYT1 sequences that differ from SEQ ID NO:5 are shown in SEQ ID NOs: 109115 and correspond to the seven most common genotype sequences for DYT1 in Cannabis; representative MYB35-15 sequences that differ from SEQ ID NO:6 are shown in SEQ ID NOs: 116-119 and correspond to the four most common genotype sequences for MYB35-15 in Cannabis; representativeMYB35-16 sequences that differ from SEQ ID NO:7 are shown in SEQ ID NOs: 120-124 and correspond to the five most common genotype sequences for MYB35-16 in Cannabis; and representativeMYB80 sequences that differ from SEQ ID NO:8 are shown in SEQ ID NOs: 125-127 and correspond to the three most common genotype sequences for MYB80 in Cannabis. As used herein, an AMS nucleic acid sequence refers to any of SEQ ID NOs: 1 or 81-88, or a sequence having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 1 or 81-88. As used herein, a bHLH91 nucleic acid sequence refers to any of SEQ ID NOs: 2 or 89-92, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 2 or 89-92. As used herein, an TM6 nucleic acid sequence refers to any of SEQ ID NOs: 3 or 93-99, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 3 or 93-99. As used herein, an STP6 nucleic acid sequence refers to any of SEQ ID NOs: 4 or 100-108, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 4 or 100108. As used herein, an DYT1 nucleic acid sequence refers to any of SEQ ID NOs: 5 or 109-115, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 5 or 109-115. As used herein, an MYB35-15 nucleic acid sequence refers to any of SEQ ID NOs: 6 or 116-119, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 6 or 116-119. As used herein, an MYB35-16 nucleic acid sequence refers to any of SEQ ID NOs: 7 or 120-124, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 7 or 120-124. As used herein, an MYB80 nucleic acid sequence refers to any of SEQ ID NOs: 8 or 125-127, or sequences having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to any of SEQ ID NOs: 8 or 125-127. It would be understood that the differences in sequences can be described by percent sequence identity. In calculating percent sequence identity, two sequences are aligned and the number of identical matches of nucleotides or amino acid residues between the two sequences is determined. The number of identical matches is divided by the length of the aligned region (i.e., the number of aligned nucleotides or amino acid residues) and multiplied by 100 to arrive at a percent sequence identity value. It will be appreciated that the length of the aligned region can be a portion of one or both sequences up to the full-length size of the shortest sequence. It also will be appreciated that a single sequence can align with more than one other sequence and hence, can have different percent sequence identity values over different aligned regions. It is noted that the percent identity value is usually rounded to the nearest integer. For example, 78.1%, 78.2%, 78.3%, and 78.4% are rounded down to 78%, while 78.5%, 78.6%, 78.7%, 78.8%, and 78.9% are rounded up to 79%. It is also noted that the length of the aligned region is always an integer. The alignment of two or more sequences to determine percent sequence identity can be performed using the algorithm described by Altschul et al. (1997, Nucleic Acids Res., 25:3389-3402) as incorporated into BLAST (basic local alignment search tool) programs, available at ncbi.nlm.nih.gov on the World Wide Web. BLAST searches can be performed to determine percent sequence identity between a nucleic acid and any other sequence or portion thereof aligned using the Altschul et al. algorithm. BLASTN is the program used to align and compare the identity between nucleic acid sequences, while BLASTP is the program used to align and compare the identity between amino acid sequences. When utilizing BLAST programs to calculate the percent identity between a sequence and another sequence, the default parameters of the respective programs generally are used. It would be appreciated that conserved regions within genes remain similar or relatively unchanged across different species or within a group of related sequences, whereas variable regions exhibit differences or divergence between different species or within a group or population. Conserved regions often correspond to functionally important domains and can be used in phylogenetic analysis to understand relationships between species or organisms, while variable regions can be used for distinguishing or differentiating individuals. Methods of Altering Gene Expression in Plants Altering the expression of genes involved in male flower reproduction via a variety of gene silencing approaches allows one to alter and control male flower production in Cannabis during breeding and cultivation. Controlling expression or function of one or more of the genes involved in male reproductive development enables sterilization or restoration of male fertility in Cannabis plants, which, in turn, allows for the prevention of hermaphroditism in Cannabis plants and the creation of male sterile lines for development of hybrid Cannabis lines. Altering gene expression of one or more of the Cannabis genes to result in non-viable pollen formation can eliminate the negative repercussions of male flower formation in the female Cannabis plant. In Cannabis, non-viable pollen cannot fertilize female flowers, and non-viable pollen protects flower harvests since flowers do not set seed in the absence of viable pollen. Non-viable pollen also avoids a loss in desirable metabolite production (e.g., cannabinoids and terpenes) that occurs upon pollen fertilization. Hence, the negative effects of Cannabis hermaphrodites are eliminated when pollen is non-viable (i.e., aborted). Further, altering gene expression of one or more of the Cannabis genes involved in pollen formation can provide both abiotic and biotic stress tolerance. Since hermaphroditism in Cannabis can occur due to biological and environmental plant stress, silencing of male flower reproduction genes involved in hermaphroditism can provide resistance to the formation of hermaphroditic male flowers that occurs as a stress response. Thus, methods of preventing hermaphroditism in Cannabis plants and methods of making a male Cannabis plant or a hermaphroditic Cannabis plant sterile are described herein. Such methods typically involve silencing expression of one or more Cannabis genes involved in pollen formation (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80). Types of gene silencing in plants include but are not limited to transcriptional gene silencing (e.g., RNA-directed DNA methylation, genomic imprinting, paramutation, transposon silencing, transgene silencing, position effect) as well as post-transcriptional gene silencing (e.g., inhibitory or interfering RNA (RNAi), clustered regularly interspaced short palindromic repeats / Cas9 (CRISPR / Cas system) and the like, and nonsense-mediated decay). RNAi is a gene silencing mechanism that relies on the homology between transcribed RNA and the target genes to cause post-transcriptional gene silencing. RNAi can be achieved with multiple types of small RNA (sRNA) such as microRNAs (miRNAs) and short interfering RNAs (siRNA) including, but not limited to, natural-antisense siRNA (nat-siRNA), trans-acting siRNA (ta-siRNA), heterochromatic siRNA (hc-siRNA), repeated-associated siRNAs (ra-siRNAs). In addition, double-stranded RNA (dsRNA) (e.g., short dsRNAs, long dsRNAs) can be used to achieve effective gene silencing. See, for example, Bennett et al., 2020, Front Plant Sci. 2020 Jun 12;11:816. The methods of preventing hermaphroditism in Cannabis plants and of making a male Cannabis plant or a hermaphroditic Cannabis plant sterile that are described herein are made possible due to the identification of the relevant genes involved in male flower formation (e.g., AMS, bHLH91, 1M6, STP6, DYT1, MYB35, MYB35, and MYB80) and are not limited by the particular manner in which a gene is silenced. In other words, the transgenic and non-transgenic methods described herein, the gene editing methods described herein, and the mutagenesis described herein are intended to be representative and are not the only ways in which a gene can be silenced. Spray-Induced Gene Silencing (SIGS) The use of RNAi, via the use of double stranded RNA (dsRNA) foliar spray, to control gene expression creates a commercial product capable of giving breeders and cultivators the tools needed to alter gene expression without the need to develop transgenic plants. SIGS has shown up to 100% gene silencing. In this disclosure, we employed SIGS via dsRNA spraying as a representative method to demonstrate the silencing effect of specific genes involved in male flower formation and hermaphroditism in Cannabis. With dsRNA spraying, foliar uptake and then cellular uptake occurs whereby double stranded RNA (dsRNA) of a targeted gene crosses the plant cell membrane and is taken up by plant cells. Although not intended to be bound by theory, once dsRNA enters the cytoplasm, plant RNAi machinery processes dsRNA into small interfering RNAs (siRNAs) that target and degrade the specific gene transcripts. These siRNAs target transcripts locally and are diffused via plant vasculature across the plant for comprehensive gene silencing. Many barriers exist to achieve efficient foliar and cellular uptake including, but not limited to, the traversing of cell walls and membranes, dsRNA degradation on surfaces prior to uptake, leaf wettability and surface tension, environmental factors such as UV exposure, humidity, pH, dose, application method, stability of the molecule, wash-off, and the trichome barrier. These barriers can be overcome from spraying of “naked” dsRNA at high pressure, or with the addition of a delivery vehicle such as, without limitation, surfactants, chemical modification and / or nanoparticles including but not limited to chitosan salts like CQAS, carbon dots, liposomes, clay nanosheets, and gold nanoparticles. While application of naked dsRNA can result in effective gene silencing, the use of surfactants, chemical modification and / or nanoparticles can achieve longer-term gene silencing effects. Genes can be targeted separately or as mixtures with simultaneous application of different dsRNA molecules in a cocktail targeting different genes or genetic regions. dsRNA application can silence genes as early as 2 hours post-spray, with signal lasting for weeks or months. In some cases, gene silencing effects can last across generations. Doses can be effective with even a single, brief application, but multiple applications over time (e.g., intermittent spraying) or steady exposure (e.g., constant spraying, soaking) can ensure steady or constant gene silencing. Plants can be sprayed with a composition containing dsRNA for any length of time (e.g., about 10 seconds to about 60 seconds) once per day or more than once per day (e.g., twice a day; five times per day; ten times per day; a plurality of times; etc.). Thus, a composition (e.g., an article of manufacture) is provided that includes one or more double-stranded RNA (dsRNA) molecules that are at least 10 nucleotides in length (e.g., at least 12, 15, 16, 17, 18, 19, 20, 25,30, 50, or 100 nucleotides in length; e.g., at least 250, 500, 1000, 1500, 2000, 2500, or 3000 nucleotides or more in length) that are complementary or have at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to at least one of the genes described herein involved in male flower development (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80) (e.g., at least two of the genes, at least three of the genes, at least four of the genes, etc.). Upon uptake by the plant, the dsRNA molecules effectively silence expression of at least one of the target genes. It would be appreciated that, the dsRNA molecules can be essentially any length (e.g., from about 10 nucleotides in length up to the full length of the gene (e.g., the full length of the mRNA)) and can be directed to different portions of a gene (e.g., the promoter, the coding sequence with or without 5’ and / or 3’ untranslated regions, introns / splice sites, etc.). As described herein, a composition including dsRNA molecules can be provided in an aqueous solution and formulated as a spray. Alternatively, a composition including dsRNA molecules can be provided in any number of other forms including, without limitation, dried or lyophilized, to be reconstituted by the ultimate or penultimate user. As an alternative to a spray, a composition including dsRNA molecules as described herein can be formulated for dipping, injecting, painting, and / or soaking. Representative dsRNA nucleic acid molecules that are complementary to the AMS gene include any of SEQ ID NO:33-43, where each of the dsRNA nucleic acid molecules effectively silence expression of the AMS gene. Representative dsRNA nucleic acid molecules that are complementary to the bHLH91 gene include any of SEQ ID NOs:44-54, where each of the dsRNA nucleic acid molecules effectively silence expression of the bHLH91 gene. Representative dsRNA nucleic acid molecules that are complementary to the TM6 gene include any of SEQ ID NOs:55-65, where each of the dsRNA nucleic acid molecules effectively silence expression of the at TM6 gene. Representative dsRNA nucleic acid molecules that are complementary to the STP6 gene include any of SEQ ID NOs:66-80, where each of the dsRNA nucleic acid molecules effectively silence expression of the STP6 gene. See Table 3 below for the sequences of these representative dsRNA nucleic acid molecules. When using SIGS, uptake of nucleic acids into plant cells can be facilitated or improved in the presence of a delivery vehicle. Without limitation, representative delivery vehicles include one or more surfactants, many different types of nanoparticles (e.g., chitosan salts (e.g., CQAS), carbon dots, liposomes, clay nanosheets, gold nanoparticles, etc.), biological vectors (e.g., viruses, bacteria (e.g., Agrobacterium spp.)), or can include buffers and / or chemical modifications. Thus, in addition to one or more dsRNAs, a composition (e.g., an article of manufacture) as described herein also can include one or more delivery vehicles (e.g., one or more surfactants, many different types of nanoparticles (e.g., chitosan salts (e.g., CQAS), carbon dots, liposomes, clay nanosheets, gold nanoparticles, etc.), biological vectors (e.g., viruses, 5 bacteria (e.g., Agrobacterium spp.)), or can include buffers and / or chemical modifications). Depending upon the delivery vehicle, a composition (e.g., an article of manufacture) as described herein further can include one or more reagents or linkers (e.g., chemical, synthetic) for attaching or conjugating the dsRNA nucleic acid molecules to a delivery vehicle. As described in more detail below, silencing of genes involved in male flower production 10 was achieved with the creation and application of foliar sprays containing dsRNA directed to different combinations of four of the genes described herein. Since it is known that dsRNA exogenous sprays are effective individually but are more effective when applied as a mixture of dsRNA from different genes, two genes were grouped into different dsRNA sprays, bound to a nanoparticle that encourages plant uptake, and sprayed onto chemically-induced hermaphroditic 15 females. For each spray, silencing of each individual gene was confirmed as was a significant reduction of pollen viability in treated hermaphroditic females, with instances of 100% pollen abortion and complete sterility. Table 3. Representative Sequences RNA ID Sequence SEQ ID NO AMS_dsRNA10* UUCAACUGUCACAUGCUCACAUGCUCUCGAGGAAUCUAUUGGGACCAGGG UUUUGAUUCCAUUGCCAGUUGCAUUGGUAGAGCUUUUUGUGAAUAAACAG AUAU CAGAAGAU CAGAAUGU GGUAGACUU CAU CACAACU CAAU 33 AMS_dsRNA9 UGCAGCAAUUUGGUUACAAUUCUGAAAGUAGGACCAGGAACGAUGCUGCC UUUCCUGAAUGUUCUCAUGAUCCAUCGGUACAUCCAGAUAAGCAUUUGGA UUCUUCAAUGGAUGCCAUGCAGCCAUCCAUGAUGAAUGACACAACCAAUU CACAUAUGCAAUUUAUGGAACAAGUAACAGAAAACAAGGAGCAGCA 34 AMS_dsRNA8 AAAG C AGAAC C AAGACU C AGAU ACAACU AAU GAG AAGG C AC C AC AGAU G G AGCCGCAAGUGGAAGUGGCUCAAAUAGAUGGAAAUGAGUUCUUUGUGAAG GUAUUCUGUGAGCACAAGCCUGGAAGAUUUGUGAGAUUGAUGGAGGCUUU GAAUUCUCUAGCUCUUGAAGUAACUAAUGCCAACGUUACUACAUUUCGAA 35 AMS_dsRNA7 AAGCAGAACCAAGACUCAGAUACAACUAAUGACAAGGCACCACAGAUGGA GCCGCAAGUGGAAGUGGCUCAAAUAGAUGGAAAUGAGUUCUUUGUGAAGG UAUUCUGUGAGCACAAGCCUGGAAGAUUUGUGAGAUUGAUGGAGGCUUUG AAUUCUCUAGCUCUUGAAGUAACUAAUGCCAACGUUACUACAUUUCGAAA 36 AMS_dsRNA6 GUAGACUUCAUCACAACUCAAUACAACAUUUUGAUGGAGCAGGAAGCCCU GAUUAGCACAAACAAUAUGGACACAAGCUUUUGUGUCAACAUUAAUGUAA UGAAUGAAAGCCAAUCAAGGCCAUUUUUGGGCAAUGAAACUGACAAAAUG GAUU CCAACAAUAAU CU GC C GGCAC CUAAUU C CACAGCAUUU GA 37 AMS_dsRNA5 AACUGACAAAAUGGAUUCCAACAAUAAUCUGCCGGCACCUAAUUCCACAG CAUUUGAGAAC CU GAACAU GUCUUAU GAC GUAU CU GUU GAU C GAACU C GU CUAUGCAAUUCCCCAAUGAGCUUGCAGCAAUUUGGUUACAAUU 38 AMS_dsRNA4 AACU CCAACAUUAAU GGUAAAAGAAACAAU GUU CU GUCU GAGACUUU GAG UCGCGACGACACUAAUUUCAGGUCUAGGCCUGAGCAUGAAAAAGUUCAAA AUGAAUUUCAUUUGGAAACAUCAAGGAGUGGAAGCAUCUCAAAGCAGAAC CAAGACUCAGAUACAACUAAUGACAA 39 AMS_dsRNA3 C CCAAGAGUUAAUU C CU GU GAACUU CUU GC C CAAUU GC CAACUU CAAU GC CUU CAGAUU CAGGAAUUUAU GCACAAAC CUU GGU CU CCAAU CAAC C CAGC U GGUUAAACUUUU CAACUGU CACAU GCU CACAU 40 AMS_dsRNA2 AACCAAUUCACAUAUGCAAUUUAUGGAACAAGUAACAGAAAACAAGGAGC AGCAUGGUAAUGAAAAGGACUCGAACAAACAGGAAGAAGGAAGAUCAAAU 41 AMS_dsRNAl CAGCAC CAU CUU CACAACCAGC C CU CUUAUU GAUUU CAUAAUUAAGUUAC AGUU CUU CAAAU GAGAUAAGAACUCAAUU CACUU CAAGCUUAAAAACAUA AUGUUUUAAAUGGAAGCAAGAUUUCUACUGAAUAAUCUAUUAAUUUUCAA UAU GUCAUAUAUAUUUAGUACU CUA 42 AMS_Full mRNA AUGGACCUGAUAAUGCAGUAUCUGAUGGAGAGGGUUAGAGCUCUAGUGGG CUCGAAAGGAUGGGAUUAUUGUGUUCUCUGGAAACUAAGUGAAGAUCAAA GGUUUAUUGAGUGGAUGAGAUGCUGUUGUGCUGGGACUGAAAGCACACAA AAUGGUGGAGAAGAAGAGCUGAUUUUUCCAGUUUCUCCAGUCCUUCCAUG CAGGGAUAC CAUAU GU CAACAC C CAAGAGUUAAUU C CU GUGAACUU CUU G CCCAAUUGCCAACUUCAAUGCCUUCAGAUUCAGGAAUUUAUGCACAAACC UUGGUCUCCAAUCAACCCAGCUGGUUAAACUUUUCAACUGUCACAUGCUC ACAUGCUCUCGAGGAAUCUAUUGGGACCAGGGUUUUGAUUCCAUUGCCAG UUGCAUU GGUAGAGCUUUUU GU GAAUAAACAGAUAU CAGAAGAU CAGAAU GUGGUAGACUUCAUCACAACUCAAUACAACAUUUUGAUGGAGCAGGAAGC CCUGAUUAGCACAAACAAUAUGGACACAAGCUUUUGUGUCAACAUUAAUG UAAUGAAUGAAAGCCAAUCAAGGCCAUUUUUGGGCAAUGAAACUGACAAA AUGGAUUCCAACAAUAAUCUGCCGGCACCUAAUUCCACAGCAUUUGAGAA C CU GAACAU GU CUUAU GAC GUAU CU GUU GAU C GAACUC GUCUAU GCAAUU CCCCAAUGAGCUUGCAGCAAUUUGGUUACAAUUCUGAAAGUAGGACCAGG AACGAUGCUGCCUUUCCUGAAUGUUCUCAUGAUCCAUCGGUACAUCCAGA UAAGCAUUUGGAUUCUUCAAUGGAUGCCAUGCAGCCAUCCAUGAUGAAUG ACACAAC CAAUU CACAUAU G CAAUUUAU G GAACAAGUAACAGAAAACAAG GAGCAGCAUGGUAAUGAAAAGGACUCGAACAAACAGGAAGAAGGAAGAUC AAAUUCAGU CU C GGAUU GUAGU GAU CAGAUU GAU GAUGAGGAUAAU GGAA AGUAUAGGAGAAAAU CAGGAAAAUCAGAU CAGU GCAAGAAC CUUUUUGCU GAGAGAAGAAGAAGGAAGAAGCUUAAU GAAAGGCU CUAUACU CUU C GGU C UUUGGUUCCCAAAAUUUCUAAGUUGGACAGGGCCUCCAUACUUGGGGAUG CAAUUGAGUAUGUAAAAGACUUACAGAGUCAGGCAAAAGAGCUUCAAGAU GAGCUAGAGGAGCAAUCAGACAAUGAUGGCCUAAACUCCAACAUUAAUGG UAAAAGAAACAAU GUU CUGU CU GAGACUUU GAGU C GCGACGACACUAAUU UCAGGUCUAGGCCUGAGCAUGAAAAAGUUCAAAAUGAAUUUCAUUUGGAA ACAUCAAGGAGUGGAAGCAUCUCAAAGCAGAACCAAGACUCAGAUACAAC UAAUGACAAGGCACCACAGAUGGAGCCGCAAGUGGAAGUGGCUCAAAUAG AUGGAAAUGAGUUCUUUGUGAAGGUAUUCUGUGAGCACAAGCCUGGAAGA UUUGUGAGAUUGAUGGAGGCUUUGAAUUCUCUAGCUCUUGAAGUAACUAA U GC CAAC GUUACUACAUUU C GAAAC CUU GUUU C CAAUGUUCUUAAAGUAG AGAAGAAGGACAGUAAAAUAGUAGU C CAAGCU GAU CACUUGAGAGACUU C UUGCUGGAGCUGACAAGGAAUCCUUCUAGAGGUUUGUCUGAGAUGUCUAA AGUUUCAGAUAAUAGCAGCAGCAUUGACUAUCAGCACCAUCUUCACAACC AGC C CU CUUAUU GAUUU CAUAAUUAAGUUACAGUU CUU CAAAU GAGAUAA GAACUCAAUUCACUUCAAGCUUAAAAACAUAAUGUUUUAAAUGGAAGCAA GAUUUCUACU GAAUAAU CUAUUAAUUUU CAAUAU GU CAUAUAUAUUUAGU ACU CUAGU GUAACU CAGCAAAGU GUAU CAGAUUU GAUGCUGAGAAU CUGU UUUUGUUUGGUC CACAGAAAUAGAAAAAGCACACUAUAUGUUUAAA 43 bHLH91_dsRNA10 GACAGAGGAAGAGAUGGCAGAGGAACCAAACACUUUGCUUCAGAGCGCCA UAGGAGAGU GCAGAU GAGU GACAAGUAC CAC GU GUUAAAGAGCUUAGUU C CUAACCCAACUAAGAAUGAUAGAGCAUCAGUAGUGGGAGAUGCGAUUGAG UACAUCAGAGAGCUGAGGAGGACAGUGAAUGAGCUAAAGAUA 44 bHLH91_dsRNA9 U CACUUUAUAAUAUAUAUGUUACAGUUUAAUAU GAAGUU GGUAGACU GAG AGACUUAUUAAUAGAUAUAAUAAAC CUU C GGUU GUAGGAAU GAU GGUUUU AGAC CU CAUU CU GACAAUU GGGGGAACAUACAUAUUAUAAGUAGAGGUUA U GGUUAUUAGUAUACUU GU GUU GUGAU CU CUUAAU 45 bHLH91_dsRNA8 CAGCAUCACAAUCAUCAUCAUCAUCAGCAGCAGCCUCAAUGGGAUAAUGA AGACAU GU CUUACAACAACAAUAAU CAU C G C CAUAAU CAACAU CAU CAG G AGCCUGCUGAUCUCCUCAGCCUCUUCCACUUGCCCAGAUGCUCGGCCACC UCCUCCUCAUCCAUCACCUUCACCAACCCGACCCCACCGAA 46 bHLH91_dsRNA7* CACAAUCAUCAUCAUCAUCAGCAGCAGCCUCAAUGGGAUAAUGAAGACAU GUCUUACAACAACAAUAAUCAUCGCCAUAAUCAACAUCAUCAGGAGCCUG CUGAUCUCCUCAGCCUCUUCCACUUGCCCAGAUGCUCGGCCACCUCCUCC U CAU CCAU CAC CUU CAC CAACC CGAC C C CAC C GAAAACAGC CAACUU 47 bHLH91_dsRNA6 AUAAUGAAGACAU GU CUUACAACAACAAUAAU CAU C GC CAUAAU CAACAU CAUCAGGAGCCUGCUGAUCUCCUCAGCCUCUUCCACUUGCCCAGAUGCUC GGCCACCUCCUCCUCAUCCAUCACCUUCACCAACCCGACCCCACCGAAAA CAGC CAACUU C C CAAACACUU 48 bHLH91_dsRNA5 UAUAUGUACAAACCUACCUAAUCACCUCCUGCCUCCCCCAGUAAGGCCAU C CAU CCAAU C C CU GCUUUAGCAGUAUACU CUUUUAUUU C CU CUACAACAG AAAGUAGAAACCUUUUACUUGAGCAGACUAGACUAGACUAUACAACA 49 bHLH91_dsRNA4 AGAGUUUGCUUACCAC CAACAU CAACAC CAG CAC CAGCAC CAG CAC CAU C AUCUUCUUCCUAAUUCUCAAGAAGAAGCAGCCAUUAAUAUGGAGCUUCAG CAACAACU C GCAUU CAACAU GGACAACU CUUACAACACU GAUAU CAA 50 bHLH91_dsRNA3 UUUGCUUACCACCAACAUCAACACCAGCACCAGCACCAGCACCAUCAUCU UCUUCCUAAUUCUCAAGAAGAAGCAGCCAUUAAUAUGGAGCUUCAGCAAC AACUCGCAUUCAACAUGGACAACUCUUACAACACUGAUAUCAACACCACU GCUCAGUUGGUGUCAUUUGAUCAUCUUCAGCAUCACAAUCAUCAUCAU 51 bHLH91_dsRNA2 CACCAGCACCAGCACCAUCAUCUUCUUCCUAAUUCUCAAGAAGAAGCAGC CAUUAAUAU GGAGCUU CAGCAACAACU C GCAUU CAACAU GGACAACU CUU ACAACACU GAUAU CAACAC CACU GCU CAGUU GGU GU CAUUU GAU CAU CUU CAGCAUCACAAUCAUCAUCAUCAUCAG 52 bHLH91_dsRNAl UUCAGCAACAACU C GCAUU CAACAU GGACAACU CUUACAACACU GAUAU C AACACCACU GCU CAGUU GGU GU CAUUU GAU CAU CUU CAGCAU CACAAU CA UCAUCAUCAUCAGCAGCAGCCUCAAUGGGAUAAUGAAGACAUGUCUUACA ACAACAAUAAUCAU C GC CAUAAU 53 bHLH91_Full mRNA UAACUCAUUUUUAUAU GUACAAACCUAC CUAAU CAC CUCCUGCCUCCCCC AGUAAGGCCAUCCAUCCAAUCCCUGCUUUAGCAGUAUACUCUUUUAUUUC CUCUACAACAGAAAGUAGAAACCUUUUACUUGAGCAGACUAGACUAGACU AUACAACACUACAAGAGAAAGGU GGGGUUUU GU GAGUCAUU GAAAGU CUU ACCAGAAAAUACAAACAAACAUGUAUAUGGAUAAUGGAAGCUGCAGCUUU GAUCCCAUUGACACAGCAGAAGCCACUGCCGUUGGUCCUGAAGAUGCCUU CAACUCCCAGCUGCCUCCUCCACCGCCACCACUACCAUCCAUGACGGGCU UUUCAGCUGCUGAUGAGAUUACUUCAGCUGCAGAAGAGCUUGCCUCAGCU GUAGAAGAGUUUGCUUACCACCAACAUCAACACCAGCACCAGCACCAGCA C CAU CAU CUU CUU C CUAAUU CU CAAGAAGAAGCAGC CAUUAAUAU GGAGC UUCAGCAACAACU C GCAUU CAACAU GGACAACU CUUACAACACU GAUAU C AACACCACU GCU CAGUU GGU GU CAUUU GAU CAU CUU CAGCAU CACAAU CA UCAUCAUCAUCAGCAGCAGCCUCAAUGGGAUAAUGAAGACAUGUCUUACA ACAACAAUAAU CAU C G C CAUAAU CAACAU CAU CAG GAG C CU G CU GAU CU C CUCAGCCUCUUCCACUUGCCCAGAUGCUCGGCCACCUCCUCCUCAUCCAU CAC CUU CAC CAAC C C GACC C CAC CGAAAACAGC CAACUU CC CAAACACUU CCCUGGCCUUUCUGGGAGAUGUUCCAAUGGGAACUGACAGCCCUCCUUCC 54 UCGUCUAUUCUGUACGACCCGCUGCUUCAUCUGAACUUACCUCCGCAGCC UCCUCUGUUUAAGGACUUGUUUCAGCCUCUCCCAAAUGGCUAUGCCUUUC C CU C CU CAU ecu CAAGAAAU ggauc cuu guu caguaau ggu guaau ggau AUGGAUGGAGAGGGGAGUGGUGGGGUUUAUCAGGAUGGACUUGAAGGAAU GCAGUUUGAAAGUGGUGUGUUGGAGUUUGCAAGGGACAGAUCUUAUAUUG ACAGAGGAAGAGAUGGCAGAGGAACCAAACACUUUGCUUCAGAGCGCCAU AGGAGAGU GCAGAU GAGUGACAAGUAC CAC GU GUUAAAGAGCUUAGUU C C UAACCCAACUAAGAAUGAUAGAGCAUCAGUAGUGGGAGAUGCGAUUGAGU ACAUCAGAGAGCUGAGGAGGACAGUGAAUGAGCUAAAGAUACUGGUGGAG AAGAAGAGGAGAGGCAUAGAGGCUAACAAGAGGCGUAAAUCAGAGGAAGA UACUGCUGGAGAGGUAGAGAAUUGCAACAUCAAGCCUGAACCUGAUCACU CCUACAUAAGGAGCUCUUGGCUGCAGAGGAAAUCUAAGGACACUGAAGUU GAUGUUCGCAUAAUCGACGAUGAGGUAACCAUCAAACUCGUCCAGAGGAA GAAGAUCAACCUCUUGCUCACUGCCUCAAGAGUUCUUGAUGAGCUUCAGC UUGAUCUUCGCCAUGUCGCUGGUGGACAUGUGGGAGACACUUACAGCUUC UUGUUCAACACCAAGAUAUAUGAAGGCUCUUCUUUGUAUGCAAGUGCCAU UGCCAACAAACUCAUGGAUGUUCUGGAUAGGCAAUUUGGAGUCAUUCUUC CACUUC CAC CUACAAAUAGCUAUUAGCUAUUU GACAAU CUAAGAU GUUAU CACUUUAUAAUAUAUAU GUUACAGUUUAAUAU GAAGUU GGUAGACU GAGA GACUUAUUAAUAGAUAUAAUAAACCUUCGGUUGUAGGAAUGAUGGUUUUA GACCUCAUUCUGACAAUUGGGGGAACAUACAUAUUAUAAGUAGAGGUUAU GGUUAUUAGUAUACUU GUGUUGU GAU CU CUUAAU GCUACUAACACUU GAG GAACACUUGAAGUUUGGCUUAUUUUCUAUGAGAAUAAUUUUCAUCUCUAU GCUAAGGCUUCCAAAGAGAGCUGGCCUAGACUUGAAGCUCAAUCUAUAUC UUUCUUUUCUUUUC TM6_dsRNA10* CGCGACCUUCGCCUGGCUUGAUGAAUUAAUCUGCACCCUAAUCAUAUGUU AGUACUACUAAUUAUAUAUAUAAUUAAUAU GU GAU CAGAGCUU GGAGU GC AUGCAUUUAUAACUCUAUCAUAAGAAUAUUAUGUUUAGUA 55 TM6_dsRNA9 CUUCUUCAUCUUGAAACCCUAGAUCUAUCUAUCUCUAUCUAUCUGUAACC AUGAUGAGUGGUUAUAAAUCUGAGCCAAACAACAAUAUUGAUCAAGACAC U CAU CAU CAAUAU CAUUAU CAU CAU CAU CAUAAU CAU CAU C GU GAU CAU G AGGAGGAAGAAGAAGAAGAU GAU CAU CAU GAU GAAAACAGCAGCAAUA 56 TM6_dsRNA8 ACCAUGAUGAGUGGUUAUAAAUCUGAGCCAAACAACAAUAUUGAUCAAGA CACU CAU CAU CAAUAU CAUUAU CAU CAU CAU CAUAAU CAU CAU C GU GAU C AU GAGGAG GAAGAAGAAGAAGAU GAU CAU CAU GAU GAAAACAG CAG CAAU AAGCAACAAAAAAUAGGAAGGGGAAAGACUCAGAUAAAGUUCAUAGAGAA 57 TM6_dsRNA7 CACU CAU CAU CAAUAU CAUUAU CAU CAU CAU CAUAAU CAU CAU C GU GAU C AU GAGGAG GAAGAAGAAGAAGAU GAU CAU CAU GAU GAAAACAG CAG CAAU AAGCAACAAAAAAUAGGAAGGGGAAAGACUCAGAUAAAGUUCAUAGAGAA CCAAACAAACAGACAAGUAACCUUUUCAAAACGAAGAAAUGGGAUUUUCA 58 TM6_dsRNA6 ACU CAU CAU CAAUAU CAUUAUCAUCAU CAU CAUAAU CAU CAU C GU GAU CA U GAG GAG GAAGAAGAAGAAGAU GAU CAU CAU GAU GAAAACAG CAG CAAUA AGCAACAAAAAAUAGGAAGGGGAAAGACUCAGAUAAAGUUCAUAGAGAAC CAAACAAACAGACAAGUAAC CUUUU CAAAAC GAAGAAAU GGGAUUUU CAA 59 TM6_dsRNA5 AUGAUGAAAACAGCAGCAAUAAGCAACAAAAAAUAGGAAGGGGAAAGACU CAGAUAAAGUU CAUAGAGAACCAAACAAACAGACAAGUAAC CUUUU CAAA ACGAAGAAAUGGGAUUUUCAAA 60 TM6_dsRNA4 ACAACAAUAUU GAU CAAGACACU CAU CAU CAAUAU CAUUAU CAU CAU CAU CAUAAU CAU CAU C GU GAU CAU GAG GAG GAAGAAGAAGAAGAU GAU CAU CA UGAUGAAAACAGCAGCAAUAAGCAACAAAAAAUAGGAAGGGGAAAGACUC AGAUAAAGUU CAUAGAGAAC CAAACAAACAGACAAGUAACCUUUU CAAAA 61 TM6_dsRNA3 CAAUAUU GAU CAAGACACU CAU CAU CAAUAU CAUUAUCAUCAU CAU CAUA AU CAU CAU C GU GAU CAU GAG GAG GAAGAAGAAGAAGAU GAU CAU CAU GAU GAAAACAGCAGCAAUAAGCAACAAAAAAUAGGAAGGGGAAAGACUCAGAU AAAGUU CAUAGAGAAC CAAACAAACAGACAAGUAAC CUUUU CAAAAC GAA 62 TM6_dsRNA2 C CAAACAACAAUAUU GAUCAAGACACU CAU GAU CAAUAU CAUUAU CAU CA U CAU CAUAAU CAU CAU C GU GAU CAU GAG GAG GAAGAAGAAGAAGAU GAU C AUCAUGAUGAAAACAGCAGCAAUAAGCAACAAAAAAUAGGAAGGGGAAAG ACU CAGAUAAAGUU CAUAGAGAACCAAACAAACAGACAAGUAAC CUUUU 63 TM6_dsRNAl GAU CAAGACACU CAU CAU CAAUAU CAUUAU CAU CAU CAU CAUAAU CAU CA UCGUGAUCAUGAGGAGGAAGAAGAAGAAGAUGAUCAUCAUGAUGAAAACA GCAGCAAUAAGCAACAAAAAAUAGGAAGGGGAAAGACUCAGAUAAAGUUC AUAGAGAAC CAAACAAACAGACAAGUAAC CUUUU CAAAACGAAGAAAU 64 TM6_Full mRNA AUAAUCUU CUU CAU CUU GAAAC C CUAGAU CUAU CUAUCU CUAU CUAU CU G UAAC CAU GAU GAGU G GUUAUAAAU CU GAG C CAAACAACAAUAUU GAU CAA GACACU CAU CAU CAAUAU CAUUAU CAU CAU CAU CAUAAU CAU CAU C GU GA UCAUGAGGAGGAAGAAGAAGAAGAUGAUCAUCAUGAUGAAAACAGCAGCA AUAAGCAACAAAAAAUAGGAAGGGGAAAGACUCAGAUAAAGUUCAUAGAG AAC CAAACAAACAGACAAGUAAC CUUUU CAAAAC GAAGAAAU GGGAUUUU CAAAAAGGCUGGUGAACUCUCUGUUCUUUGUGAUGCUAAGGUCUCUCUUA UUAUUGU C C CUAACAAUAAUAAACU C CAU GAAUU CAUUACU C CU GGAGC C AAUACUAAGGAAAU GAUUGAUCU GUAU CAAAGAAC CAAAGGU GUU GAU CU GUGGAAGUCACAGUACGAGGCAAUGCAACAAACCUUGCGCCAACUCAAAG CUAAAAACUUUGAACUGAGGACACAGAUCAGACAGAGAUUGGGUUAUGAU UUGAAUGAUCUGAGUUUUCAUGAGCUAAGUGAUCUUGAGAAUAGUAUGCU UUCUUCU GU GGAAGC CAUAC GC CACAGAAAGAAU CACAAGAU CAAAACU C AGGCUGAAACCACCAAGAAAAGGAACAAGAGCUGGGAGGAAAGAAACUCA GUCCUCAUGCAUGAAAUUCAUGCAAGGUGUGAAGAUCCACCAUAUGGUUU AGUGGAGGAUAAUGUAGAAGGAGGAGGAGGAGGAGGGUAUGAAUCAUCAG CAGUUGCAUUAGCUAAUGGAGCUUCCAAUCUCUAUGCCUUCCGCCACCAC CACAACGGCGGAGGAAGCUUAAUCACCACCAGUCACUCCGCUCUUCACAG UGGCACCGGCGGUGGUGGUAGUGGCGGUGCCUAUGAAUCCUUUGAUCUCC GCGACCUUCGCCUGGCUUGAUGAAUUAAUCUGCACCCUAAUCAUAUGUUA GUACUACUAAUUAUAUAUAUAAUUAAUAU GU GAU CAGAGCUU GGAGU GCA U GCAUUUAUAACU CUAU CAUAAGAAUAUUAU GUUUAGUAUGUACUACUUU UGGU 65 STP6_dsRNA14 AUCAUGUUUUAUGCUCCUGUACUAUUUCAAACCAUCGGUUUCGGGAGUGA UGCAUCCCUUUUGUCAUCGGCAGUCACAGGUAGUGUGAAUGUAGCAUCAA CUUUUGUUGCAAAUAUUAUUGUUGAUAAGGCCGGAAGAAAGAGUUUACUG AUU GAAGCAGCU GUU CAAAU GUU CAUU GCACAGU GAAAA 66 STP6_dsRNA13 AGGAAUUAAC GUAAU CAUGUUUUAU GCU C CU GUACUAUUUCAAAC CAU C G GUUUCGGGAGUGAUGCAUCCCUUUUGUCAUCGGCAGUCACAGGUAGUGUG AAUGUAGCAUCAACUUUUGUUGCAAAUAUUAUUGUUGAUAAGGCCGGAAG AAAGAGUUUACU GAUU GAAGCAGCU GUU CAAAU GUU CAUUGCACAGU GAA 67 STP6_dsRNA12 CAGGAAUUAAC GUAAU CAU GUUUUAU GCU C CU GUACUAUUU CAAAC CAU C GGUUUCGGGAGUGAUGCAUCCCUUUUGUCAUCGGCAGUCACAGGUAGUGU GAAUGUAGCAUCAACUUUUGUUGCAAAUAUUAUUGUUGAUAAGGCCGGAA GAAAGAGUUUACU GAUU GAAGCAGCU GUU CAAAU GUUCAUU GCACAGU GA 68 STP6_dsRNAll CACAGGAAUUAAC GUAAUCAUGUUUUAU GCU C CU GUACUAUUU CAAAC CA UCGGUUUCGGGAGUGAUGCAUCCCUUUUGUCAUCGGCAGUCACAGGUAGU GUGAAUGUAGCAUCAACUUUUGUUGCAAAUAUUAUUGUUGAUAAGGCCGG AAGAAAGAGUUUACU GAUU GAAGCAGCU GUU CAAAU GUU CAUU G 69 STP6_dsRNA10 U CAACAAUU CACAGGAAUUAAC GUAAU CAU GUUUUAUGCUC CU GUACUAU UUCAAACCAUCGGUUUCGGGAGUGAUGCAUCCCUUUUGUCAUCGGCAGUC ACAGGUAGU GU GAAU GUAGCAU CAACUUUU GUU GCAAAUAUUAUU GUU GA UAAGGCCGGAAGAAAGAGUUUACUGAUUGAAGCAGCUGUUCAAAUGUUCA 70 STP6_dsRNA9 AGAAUAUGAAAUGAUCACACAUGCCACUGAAUUAGCUUCUCAAGAGAAGC AUU CUUAUAGAAAACUAAU GCAU CGUU C CAACAGAC CU CAACUUUU GGUA ACCGUGGCCCUGCAAAUUUUUCAACAAUUCACAGGAAUUAACGUAAUCAU GUUUUAU GCU C CU GUACUAUUU CAAAC CAU 71 STP6_dsRNA8 AGGAGU GGAAGAU GUU CAAAAAGAAUAU GAAAU GAU CACACAU GC CACU G AAUUAGCUUCUCAAGAGAAGCAUUCUUAUAGAAAACUAAUGCAUCGUUCC AACAGAC CU CAACUUUU GGUAAC CGU GGC C CU GCAAAUUUUU CAACAAUU CACAGGAAUUAAC GUAAUCAUGUUUUAU GCU C CU GUACUAUUU CAAAC CA 72 STP6_dsRNA7 U C GAGGAGU G GAAGAU GUU CAAAAAGAAUAU GAAAU GAU CACACAU G C CA CUGAAUUAGCUU CU CAAGAGAAGCAUU CUUAUAGAAAACUAAU GCAUC GU U CCAACAGAC CU CAACUUUU GGUAAC C GU GGC C CU GCAAAUUUUU CAACA AUUCACAGGAAUUAACGUAAUCAUGUUUUAUGCUCCUGUACUAUUUCAAA 73 STP6_dsRNA6 UACAAU GAU GAACAAGC GGCAACAAAAUAAUUUAUUUAGUUUUUUU GUAG UUAACUUAUUUAGUUUUUAGUUU GGU GUUUUUU CU GGAUUGAAUUUU GAA UUUU 74 STP6_dsRNA5 UGCCAUAUGCAAUCGGGAAUAUUCUUCUUUUUUGCCGCUUGGGUUCUCGU UAUGUUCUUGUUUGCCAUUUUUCUUCUGCCGGAAACGAAAGGAAUUCCAA UUGAUGAAAUGGUUGACAGGGUAUGGAAGAAGCAUUGGUAUUGGAAGAAA UAUUACAAU GAU GAACAAGC GGCAACAAAAUAAUUUAUUU 75 STP6_dsRNA4 AAGCUUUCUUAACUAUGUUGUGCCAUAUGCAAUCGGGAAUAUUCUUCUUU UUUGCCGCUUGGGUUCUCGUUAUGUUCUUGUUUGCCAUUUUUCUUCUGCC GGAAACGAAAGGAAUUCCAAUUGAUGAAAUGGUUGACAGGGUAUGGAAGA AGCAUUGGUAUUGGAAGAAAUAUUACAAUGAUGAACAAGCGGCAACAAA 76 STP6_dsRNA3 UCAAGCUUUCUUAACUAUGUUGUGCCAUAUGCAAUCGGGAAUAUUCUUCU UUUUUGCCGCUUGGGUUCUCGUUAUGUUCUUGUUUGCCAUUUUUCUUCUG CCGGAAACGAAAGGAAUUCCAAUUGAUGAAAUGGUUGACAGGGUAUGGAA GAAGCAUUGGUAUUGGAAGAAAUAUUACAAUGAUGAACAAGCGGCAACAA 77 STP6_dsRNA2 CUCAAGCUUUCUUAACUAUGUUGUGCCAUAUGCAAUCGGGAAUAUUCUUC UUUUUUGCCGCUUGGGUUCUCGUUAUGUUCUUGUUUGCCAUUUUUCUUCU GCCGGAAACGAAAGGAAUUCCAAUUGAUGAAAUGGUUGACAGGGUAUGGA AGAAGCAUUGGUAUUGGAAGAAAUAUUACAAUGAUGAACAAGCGGCAA 78 STP6_dsRNAl* UCGCUCAAGCUUUCUUAACUAUGUUGUGCCAUAUGCAAUCGGGAAUAUUC UUCUUUUUUGCCGCUUGGGUUCUCGUUAUGUUCUUGUUUGCCAUUUUUCU UCUGCCGGAAACGAAAGGAAUUCCAAUUGAUGAAAUGGUUGACAGGGUAU GGAAGAAGCAUUGGUAUUGGAAGAAAUAUUACAAUGAUGAACAAGCGGCA 79 STP6_Full mRNA UUAGUUUCAUUUUUUUUUUUACUGAGUUAAUAAUCGAAACCUUUCAUUCAU UUUUUUUCUUACUGUAAGUAUGACGGGGGCAGUGAUGGUUGAGUCCGGCGG UAGCAGCGGCGGUGACUUUCCCGCAAAACUUACGAAACAAGUGAUCGUGUG CAGCAUAAUCGCUGCAUGCGGCGGCCUGAUGUUCGGCUAUGACAUCGGCAU UUCGGGUGGGGUGACAUCGAUGGACGAUUUUUUAAUUAAAUUUUUUCCAAU CGU GUAU G CUAAAAAG CAC GAAGU GAAAGAAAAUAACUAUU G CAAAUAC GA UAAUCAGUAUUUGCAAUUAUUCACUUCGUCGCUUUAUUUAGCGGCCAUUUU GGCUUCUUUUAUAGCUUCGAUAACUUGUAAGGCUUUCGGGCGAAAGGUGAC CAUUCAAGCUGCGUCGGUGUUCUUCGUCACCGGUUCGGUUUUGAAUGCGGC UGCCAAAGAUCUCGGAAUGUUGAUUGCUGGAAGGAUUUGUCUUGGAAUCGG UGUUGGAUUUGGCAAUCAGGCAGUUCCACUAUUUAUAUCUGAAAUUGCACC AACAAGAUAU C GAGGAGCU CUCAACAU CU GUUUU CAAUUAUUAAUUACAGU U GGAAUUUU GUU GGC CAAUUUC GUAAAUUAU GGUACAU CAC GAAU C CAU C C AUACGGAUGGAGAAUCUCAUUGGGAGGAGCUGGAGUCCCCGCUCUAAUAUU ACUGGUGGGAUCUUUACUGAUUGUAGAGACCCCAACCAGUCUUAUCGAGCG AGGUCACAUUGAGGAAGGUUUAAAAAUUUUAAGAAAAAUUCGAGGAGUGGA AGAU GUU CAAAAAGAAUAU GAAAUGAU CACACAU GC CACUGAAUUAGCUU C U CAAGAGAAGCAUU CUUAUAGAAAACUAAU GCAU C GUU C CAACAGAC CU CA ACUUUUGGUAACCGUGGCCCUGCAAAUUUUUCAACAAUUCACAGGAAUUAA CGUAAUCAUGUUUUAUGCUCCUGUACUAUUUCAAACCAUCGGUUUCGGGAG UGAUGCAUCCCUUUUGUCAUCGGCAGUCACAGGUAGUGUGAAUGUAGCAUC AACUUUU GUU GCAAAUAUUAUU GUU GAUAAGGC C GGAAGAAAGAGUUUACU GAUUGAAGCAGCUGUUCAAAUGUUCAUUGCACAGUCUAUAAUGGGUGGUAU ACU C GCAAAAUAU CU CACAGCUACAAGUACAAU GC C GGAUAAAUACGCAUG GAUAGUAUUAAU CUUAAUUU GUAUUUUU GUAU CU GGAUUUGCAU GGU CAU G 80 GGGUCCUCUUGGAUGGUUGAUUCCAAGUGAAAUAUUCCCAUUGGAGGUUCG AAAUGCAGGAUUCUUCUUUGCGGUGUCCACGAACAUGUUGUUUACUUUUCU UAUCGCUCAAGCUUUCUUAACUAUGUUGUGCCAUAUGCAAUCGGGAAUAUU CUUCUUUUUUGCCGCUUGGGUUCUCGUUAUGUUCUUGUUUGCCAUUUUUCU UCUGCCGGAAACGAAAGGAAUUCCAAUUGAUGAAAUGGUUGACAGGGUAUG GAAGAAGCAUUGGUAUUGGAAGAAAUAUUACAAUGAUGAACAAGCGGCAAC AAAAUAAUUUAUUUAGUUUUUUUGUAGUUAACUUAUUUAGUUUUUAGUUUG GUGUUUUUUCUGGAUUGAAUUUUGAAUUUUGAAUUUUUGGAUU * data presented in Examples As described below in more detail, Cocktail 1 and Cocktail 2 demonstrated a 63% and 65% rate of pollen abortion, respectively, on average. No other observed phenotypic changes were observed in treatment plants; plants in all treatment groups remained healthy. Transgenic Plants Methods of genetically engineered plants to effectuate gene silencing are known in the art. As described herein, such methods can include genetically engineering plants to silence one or more of the genes involved in male flower production (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80). Thus, methods of introducing nucleic acids into Cannabis cells are provided. Typically, nucleic acids having at least 80% sequence homology to any of the sequences shown in SEQ ID NOs: 1 or 81-88 (AMSy SEQ ID NOs: 2 or 89-92 (bHLH9Pg SEQ ID NOs: 3 or 93-99 (TM6y SEQ ID NOs: 4 or 100-108 (STP6y SEQ ID NOs: 5 or 109-115 (DYTiy SEQ ID NOs: 6 or 116119 (MYB35-15)', SEQ ID NOs: 7 or 120-124 (MYB35-16)', or SEQ ID NOs: 8 or 125-127 (MYB80), or a portion thereof, or nucleic acids having a sequence that is complementary to any of the sequences shown in SEQ ID NOs: 1 or 81-88 (AMS)', SEQ ID NOs: 2 or 89-92 (bHLH91), SEQ ID NOs: 3 or 93-99 (TM6)-, SEQ ID NOs: 4 or 100-108 (STP6)', SEQ ID NOs: 5 or 109-115 (DYTiy SEQ ID NOs: 6 or 116-119 (MYB35-15)' SEQ ID NOs: 7 or 120-124 (MYB35-J6y or SEQ ID NOs: 8 or 125-127 (MYB80), or a portion thereof, can be introduced into Cannabis cells. Introducing such a nucleic acid into Cannabis cells can effectively silence expression of the respective gene(s) in those cells or in a subsequently produced plant (e.g., a transgenic plant), and it would be understood that the methods described herein are not limited to any particular mechanism by which the gene silencing takes place. Simply by way of example, transgenic plants are provided that express at least one nucleic acid (e.g., a transgene) at least 10 nucleotides in length (e.g., at least 12, 15, 16, 17, 18, 19, 20, 25, 30, 50, or 100 nucleotides in length; e.g., at least 250, 500, 1000, 1500, 2000, 2500, or 3000 nucleotides or more in length) having at least 80% sequence identity (e.g., at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity) to at least one of the nucleic acids described herein (e.g., any of SEQ ID NOs :1 or 81-88 (AMS); SEQ ID NOs: 2 or 89-92 (bHLH91); SEQ ID NOs: 3 or 93-99 (TM6); SEQ ID NOs: 4 or 100-108 (STP6); SEQ ID NOs: 5 or 109-115 (DYT1); SEQ ID NOs: 6 or 116-119 (MYB35-15); SEQ ID NOs: 7 or 120-124 (MYB35-16); or SEQ ID NOs: 8 or 125-127 (MYB80)). Upon transient or stable expression of the nucleic acid, the inhibitory or interfering RNA (RNAi) process is initiated, as a consequence of the expression leading to dsRNA, which ultimately results in the silencing of the corresponding endogenous nucleic acid. As described herein, such transgenic plants exhibit a greater amount of non-viable pollen relative to, e.g., a plant lacking or not expressing the nucleic acid. Methods of introducing a nucleic acid (e.g., a transgene) into plant cells are known in the art and include, for example, Agrobacterium-mediated transformation, agroinfiltration, particle bombardment, microinjection, polyethylene glycol-mediated transformation (e.g., of protoplasts), liposome-mediated DNA uptake, or electroporation. It would be understood that, in some instances, achieving stable or transient transformation is dependent upon the particular vector or construct used. Following stable transformation, transgenic plant cells can be regenerated into transgenic plants. It would be appreciated that transgenic plants can be screened for male flower development, viability of pollen, etc., compared to a corresponding plant not expressing or containing the nucleic acid. Gene Editing Any number of gene editing techniques can be used to specifically alter the sequence of one or more of the genes described herein involved in male flower development (e.g., AMS, bEILH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80) in a Cannabis cell include, without limitation, gene replacement (e.g., using homologous recombination or homology-directed repair), gene editing, base editing, or prime editing. In some cases, clustered regularly interspaced short palindromic repeat (CRISPR) / CRISPR-associated (Cas) nuclease (CRISPR / Cas) gene editing (e.g., therapeutic gene editing) techniques can be used to edit a gene (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80) present in a Cannabis cell. CRISPR / Cas molecules are components of a prokaryotic adaptive immune system that is functionally analogous to eukaryotic RNA interference, using RNA base pairing to direct nucleic acid cleavage resulting in double stranded breaks (DSBs) about 3-4 nucleotides upstream of a protospacer adjacent motif (PAM) sequence. Directing nucleic acid DSBs with the CRISPR / Cas system requires two components: a Cas nuclease, and a guide RNA (gRNA) targeting sequence directing the Cas to cleave a target DNA sequence. A CRISPR / Cas system can include any appropriate Cas nuclease. A number of Cas nucleases (e.g., Cas9, Cas 12) are known in the art. In some cases, a TALEN system can be used (e.g., can be introduced into Cannabis cells) to edit the sequence of a gene (e.g., AMS, bHLH91, TM6, STP6, DYTl, MYB35, MYB35, and MYB80) in a Cannabis cell. For example, an engineered transcription activator-like (TAL) effector DNA binding domain targeting sequence can be fused to a nuclease to create a TALEN that can create nucleic acid double-stranded breaks (DSBs) at or near the sequence targeted by the TAL effector DNA binding domain. Directing nucleic acid DSBs with the TALEN system requires two components: a nuclease, and TAL effector DNA-binding domain directing the nuclease to a target DNA sequence. A TALEN system can include any appropriate nuclease. Thus, methods of introducing (e.g., by transformation) one or more constructs that include gene editing components (e.g., TALEN technology, zinc-finger nuclease technology, CRISPR technology, or variations thereof (e.g., FANZOR, NICER, etc.)) into Cannabis cells are provided herein. Such methods enable at least one of the Cannabis genes selected from (e.g., AMS, bHLH91, TM6, STP6, DYTl, MYB35, MYB35, and MYB80) to be edited, so as to silence one or more of the genes involved in male flower production. Mutagenesis Methods of mutagenesis are known in the art, and methods of making a plant having a mutation in its genome are known in the art. Mutations can be random mutations or targeted mutations. For random mutagenesis, plant cells can be mutagenized using, for example, a chemical mutagen, ionizing radiation, or fast neutron bombardment. Representative chemical mutagens include, without limitation, nitrous acid, sodium azide, acridine orange, ethidium bromide, and ethyl methane sulfonate (EMS), while representative ionizing radiation includes, without limitation, x-rays, gamma rays, fast neutron irradiation, and UV irradiation. The dosage of the mutagenic chemical or radiation is determined experimentally for each type of plant tissue such that a mutation frequency is obtained that is below a threshold level characterized by lethality or reproductive sterility. The number of Mi generation seed or the size of Mi plant populations resulting from the mutagenic treatments are estimated based on the expected frequency of mutations. A mutation can be a point mutation, an insertion, a deletion, an inversion, a translocation, or combinations thereof. One or more nucleotides within a gene can be mutated to alter the expression and / or function of the encoded polypeptide, relative to the expression and / or function of the corresponding wild type polypeptide. In addition, a mutation in a single nucleotide can create a stop codon, which would result in a truncated polypeptide and, depending on the extent of truncation, cause partial to complete loss-of-function. Further, a mutation in a promoter sequence can enhance, reduce or completely eliminate the gene expression in a plant comprising the mutation. A mutation in a promoter sequence can alter or eliminate the binding or recognition site of a transcription factor or of the polymerase enzyme, or a mutation in a promoter sequence can alter or eliminate the function of an enhancer, an activator or the like, or a repressor, a silencer or the like. Mutations in a promoter sequence can result in altered or absent transcription, or production of a less-than-functional or non-functional transcript. A less-than-functional or non-functional transcript can result from improper expression (e.g., expressed in the wrong place or at the wrong time), or from degradation of the transcript. Alternatively, a mutation in a promoter sequence may allow transcription to take place but may interfere with or eliminate the ability of the transcript to be translated into protein. Mutations in a coding sequence can result in insertions of one or more amino acids, deletions of one or more amino acids, and / or non-conservative amino acid substitutions in the encoded polypeptide. Insertion or deletion of amino acids in a coding sequence, for example, can disrupt the conformation of the encoded polypeptide. Amino acid insertions or deletions also can disrupt sites important for recognition of a binding ligand or for activity of the polypeptide. It is known in the art that the insertion or deletion of a larger number of contiguous amino acids is more likely to render the gene product non-functional, compared to a smaller number of inserted or deleted amino acids. In addition, one or more mutations can change the localization of a polypeptide, introduce a stop codon to produce a truncated polypeptide, or disrupt an active site or domain (e.g., a catalytic site or domain, a binding site or domain) within the polypeptide. Non-conservative amino acid substitutions can replace an amino acid of one class with an amino acid of a different class. Non-conservative substitutions can make a substantial change in the charge or hydrophobicity of the gene product. Non-conservative amino acid substitutions can also make a substantial change in the bulk of the residue side chain, e.g., substituting an alanine residue for an isoleucine residue. Examples of non-conservative substitutions include a basic amino acid for a non-polar amino acid, or a polar amino acid for an acidic amino acid. Thus, methods of exposing Cannabis cells to a mutagen are provided. After mutagenesis, plants having a mutation in one or more of the genes (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80) can be selected, where the mutation effectively silences expression of at least one of the genes. Genetic Markers of Hermaphroditism It would be appreciated that any of the genes described herein (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80), or a particular domain therefrom (e.g., an activation domain) or a SNP or other type of variation, can be used, for example, as markers of hermaphroditism to predict male sterility or hermaphroditism resistance status of a plant or cultivar, to restore male functionality, or to create new plants or products. It would be appreciated that any of the genes described herein (e.g., AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, wdMYB80), or a particular domain therefrom (e.g., an activation domain) or a SNP or other type of variation, can be used, for example, in marker-assisted breeding. For example, DNA fingerprinting or similar technologies can be used in a marker-assisted breeding program to transfer or breed one or more variant alleles into other lines, varieties or cultivars, as known in the art. Progeny of the cross can be screened for the domain, SNP or other variation using methods described herein, and plants having the domain, SNP or other variation can be selected. For example, plants in the F2 or backcross generations can be screened using a marker developed from a sequence described herein or a fragment thereof, using any one of known techniques. Plants also can be screened for the domain, SNP or other variation, and those plants having the domain, SNP or other variation can be selected. Plants identified as possessing the domain, SNP or other variation and / or a desired phenotype can be backcrossed or self-pollinated to create a second population to be screened. Backcrossing or other breeding procedures can be repeated until the desired phenotype of the recurrent parent is recovered. In accordance with the present invention, there may be employed molecular biology, microbiology, biochemical, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. The invention will be further described in the following examples, which do not limit the scope of the methods and compositions of matter described in the claims. EXAMPLES Example 1—Identification of Pollen Formation Genes Using Differential Gene Expression and Co-Expression Analysis from Hermaphroditic, Male and Female Flowers Plant Material and Sample Collection. Female flowers and natural (non-chemically induced) hermaphroditic flowers from the same plants were collected in three biological replicates per plant from the field and stored in RNAlater. The amount of hermaphroditic flower present on each plant was noted per plant. Natural hermaphroditic samples and paired female flowers from the same plant were selected for RNA extraction only from the plants expressing the most hermaphroditism. Separately, male flowers from a common garden experiment were collected in three biological replicates, flash frozen in liquid nitrogen, and stored at -80°C until processing. RNA isolation and Sequencing'. RNA was extracted from floral tissue only (i.e., as much non-flower tissue (e.g., leaf) as possible was removed). RNA was isolated using Qiagen RNeasy Plant Mini Prep Kit following manufacturer's protocol. RNA was quantified using Thermo Fisher Qubit 4 (Waltham, Massachusetts, USA). CD Genomics (Shirley, New York, USA) prepared the samples for standard Poly-A enrichment and stranded cDNA library preparation with quality control performed using RIN scores from an Agilent 4200 Tapestation (Santa Clara, California, USA). Samples were barcoded and RNA-Seq was performed. Sequencing Read Processing: Sequencing adapters and low-quality reads (Q score < 20) were removed from the raw sequencing reads using Trimmomatic software version 0.39 (Bolger et al. 2014) with parameters: “LEADING:20”, “TRAILING:20”, “SLIDINGWINDOW:4:15”, “AVGQUAL:20”, “MINLEN:35”. On average, 71.7 million reads were retained per sample for alignments. The remaining high-quality reads from each sample were aligned to the indexed CBDRx genome, also known as cslO genome (Grassa et al. 2021), using Hisat2 software with default parameters (Kim et al. 2019). The alignment files in BAM (binary alignment map) format were processed with Samtools software (Heng et al. 2009) to remove any unmapped reads, and the single mapped reads whose mate was not mapped. The remaining properly paired and aligned sequence reads from each sample were counted for individual genes in the Cannabis CBDRx (cslO) genome using HTSeq count software (Anders, Pyl and Huber, 2014). CslO is considered the most comprehensive reference genome available, but any Cannabis genome could be used with this pipeline. Identification of Differentially Expressed Genes'. The gene-specific read counts from each sample were employed to identify differentially expressed genes between male flowers, female flowers and hermaphroditic flowers across multiple cultivars using DESeq2 package version 1.42.0 (Love, Huber and Anders 2014). The male, female, and hermaphroditic samples were compared pairwise (Herm vs. Female, Herm vs. Male, Male vs. Female) with a generalized linear model to obtain a p-value of significance and log2Fold change for each gene model across the tissues. The model used replicated data to normalize any expression variation due to sampling error. The expression statistics (p-value and log2Fold) were obtained for each gene in the CBDRx genome and the genes with a p-value of <0.05 and a minimum log2Fold threshold of 1.5 were identified as differentially expressed. The genes were designated as up-regulated and down-regulated in the hermaphroditic samples based on their relative normalized expression scores in the male or female samples and in the context of male vs female expression differences. Identifying Co-Expression Modules for Hermaphroditism'. The normalized expression values obtained from DESeq2 were used to construct a co-expression network for all the annotated genes in the cslO genome using weighted correlation network analysis (WGCNA) R package (Langfelder and Horvath 2008). The co-expression modules were identified using the one-step network building and module detection method in the WGCNA software. WGCNA connects all data variables in the expression dataset to identify networks with similar expression patterns across samples. Initially, an unsigned topological overlap matrix was built to define a threshold for network detection, which led to a network threshold power of 9 to generate a coexpression network for hermaphroditic, male and female flower samples. In addition, parameters including a minimum module size equal to 30, and a branch merge cut height of 0.25 were used to identify the co-expression network modules. The resulting co-expression modules were visualized using heatmaps in R statistical software (Perry 2023) and in Cytoscape version 3.9.1 (Shannon et al. 2003). This analysis identified two specific modules that exhibited clear expression pattern differences in hermaphroditic samples from the male and female Cannabis samples. Identifying Candidate Genes for Male Flower Reproduction. The differential expression analysis identified 2075, 3177, and 1436 significant (p < 0.05) differentially expressed genes (DEGs) between hermaphroditic vs. female, hermaphroditic vs. male, and female vs. male samples, respectively, across multiple genetic backgrounds. Out of the 2075 hermaphroditic vs. female DEGs, 1075 genes were upregulated in the hermaphroditic samples, and a maximum log2Fold change of 12.56 was observed. Similarly, 1591 DEGs showed upregulation in the hermaphroditic samples against the natural male samples with a maximum log2Fold change of 11.54. Collectively, this dataset of DEGs was called “LW-DEGs” for further reference. Several DEGs were present in two different co-expression modules. These results highlight that hermaphroditic flower production and natural female flower production occurs through different pathways expressed in a concerted fashion. Moreover, this analysis provides a second layer of evidence to shortlist the candidate genes for hermaphroditic flower production. These analyses identified 75 genes, whose expression was different in the hermaphroditic samples compared to the male and female samples. The annotations for these 75 genes were retrieved from the cslO genome annotations. If a gene was not annotated in the cslO genome, it was manually annotated using the Basic Local Alignment Search Tool (BLAST) tool in the National Center for Biotechnology Information (NCBI) database and the fgenesh software (Solovyev et al. 2006). These selected 75 genes were categorized into classes based on their annotated role in flowering or flowering related pathways. Identification and Validation of Candidate Genes'. To further validate the expression differences of the 75 DEGs between hermaphroditic samples and male and female samples, we retrieved public RNA-Seq dataset from the male (M), female (F), and silver thiosulfate (STS) induced male (IM) samples, which are artificially produced hermaphroditic male flowers on female plants from Adal et al. (2021). The raw sequencing reads for these samples were downloaded from the NCBI Sequence Read Archive (SRA) database using the BioProject accession PRJNA669389, BioSample accessions SAMN16447877-SAMN16447891, and SRA accessions SRR12831863-SRR12831877. The raw read sequences were processed and analyzed to identify DEGs between induced hermaphroditic and male and female samples using the previously described methods. These DEGs are hereafter referred to as “Adal-DEGs”. In addition, we crosschecked DEGs from a similar study (McKay 2021) as an additional validation measure for gene expression differences observed in the LW-DEGs and Adal-DEGs. Genes shared between the previously selected 75 LW-DEGs and Adal-DEGs were identified as candidate genes for further analysis. Based on these comparisons and an initial literature search of genes involved in flower formation, reproductive systems, pollen development, male sterility, and hermaphroditism in other species, a set of 10 candidate genes were selected for further analysis. Low or lack of expression of a gene in hermaphrodites and in males with relatively higher expression in females was confirmed for each gene to be retained as an acceptable candidate. In addition to the top 10 selected genes, we performed a literature search to identify additional putative candidate genes in both LW-DEGs and Adal-DEGs datasets based on their function in male reproductive organs in other species. Any genes whose mutant showed phenotypic defects in the male reproductive parts in various species and which showed differential expression in either LW-DEGs or Adal-DEGs were added to the list of putative candidate genes. A list of 14 candidate genes was retained. We further filtered out candidate genes if a gene showed more than one match to any off-target regions in cs 10 or a genetically dissimilar Cannabis genome, Sour Pear. Additionally, genes exhibiting non-relevant or undesirable phenotypic effects outside of male reproductive function in other species were removed. Genes were kept for future analysis if they satisfied the following criteria: 1) genes were specifically expressed in a male flower, whether hermaphroditic or male 2) mutated genes expressed a desirable phenotype only in the male flower, and 3) genes exhibited a single BLAST hit within the Cannabis genome. Example 2—Identification of Relevant Genes and Evaluation of Genetic Variation Therein A total of eight genes - AMS (ABORTED MICROSPORES), bHLH91 (Basic-loop-basic helix 91 - homolog to EAN1, ATM1, bHLH10'89 / 91), STP6 (SUGAR TRANSPORT PROTEIN 6), TM6 (Agamous-Like TOMATO MADS BOX GENE 6), DYT1 (DYSFUNCTIONAL TAPETUM 1), as well as MYB35-15, MYB35-16 and MYB80 (homologous to DEFECTIVE IN MERISTEM DEVELOPMENT AND FUNCTION 1) were retained from candidate gene analysis for functional validation. These genes were expressed at a higher level in the hermaphroditic and / or male flowers but largely minimally expressed or showed no expression in the female flowers, making RNAi an ideal approach to decrease their expression and test their 5 involvement in male reproduction in Cannabis. Annotations for these genes were checked to confirm their identities and gene structure through NCBI nucleotide BLAST database and the fgenesh software (Solovyev et al. 2006). To evaluate the genetic variation in the eight genes identified, we used whole genome sequences for 137 Cannabis accessions within the LeafWorks database. Trimmomatic software 10 version 0.39 (Bolger et al. 2014) was used to filter out low quality read sequences using the following parameters: LEADING:20 TRAILING:20 SLIDINGWINDOW:4:15 AVGQUAL:20 MINLEN:35. The high-quality reads were aligned against the cslO genome using minimap2 software version 2.24 (Li, 2018). The alignment files were processed to remove PCR duplicates using 15 Picard software version 2.27.4 (picard: Broad Institute, 2018). The resulting alignment files were used to call variants using “HaplotypeCaller”, “GenomicsDBImport”, and “GenotypeGVCFs” plugins in the GATK software version 4.3.0.0 (McKenna et al. 2010). The variants were retrieved in a Variant Call Format (VCF) file. The resulting VCF file was scanned based on the genomic coordinates for AMS, bHLH91, STP6, and TM6 genes to identify single nucleotide 20 polymorphisms (SNPs) and Insertions / Deletions (INDELs) in the respective genes. Very little sequence variation was found in the genes, indicating they are conserved across many Cannabis accessions. Briefly, AMS had 8 SNPs, bHLH91 had 2 SNPs, STP6 had 5 SNPs, and TM6 had 1 SNP. See Table 4. In addition, a single 1 bp deletion in TM6 in a single cultivar, 680_S27, was identified. Table 4. SNPs Gene-SNP# SNP position in CBDRx genome SNP position in CBDRx gene Position in MSA AMS (start) 7649547 1 AMS-SNP1 7649492 -55 6097 AMS-SNP2 7649496 -51 6101 AMS-SNP3 7649506 -41 6111 AMS-SNP4 7649561 14 6166 AMS-SNP5 7649595 48 6200 AMS-SNP6 7649603 56 6208 AMS-SNP7 7649606 59 6211 AMS-SNP8 7649611 64 6216 AMS-SNP9 7649612 65 6217 AMS-SNP10 7649643 96 6248 AMS-SNP11 7649663 116 6268 AMS-SNP12 7649677 130 6282 AMS-SNP13 7649678 131 6283 AMS-SNP14 7649687 140 6292 AMS-SNP15 7649695 148 6300 AMS-SNP16 7649707 160 6312 AMS-SNP17 7649725 178 6330 AMS-SNP18 7649737 190 6342 AMS-SNP19 7649740 193 6345 AMS-SNP20 7649756 209 6361 AMS-SNP21 7649760 213 6365 AMS-SNP22 7649761 214 6366 AMS-SNP23 7649762 215 6367 AMS-SNP24 7649763 216 6368 AMS-SNP25 7649764 217 6369 AMS-SNP26 7649766 219 6371 AMS-SNP27 7649768 221 6373 AMS-SNP28 7649772 225 6377 AMS-SNP29 7649793 246 6398 AMS-SNP30 7649859 312 6467 AMS-SNP31 7649891 344 6499 AMS-SNP32 7650049 502 6661 AMS-SNP33 7650118 571 6730 AMS-SNP34 7650127 580 6739 AMS-SNP35 7650340 793 6952 AMS-SNP36 7650374 827 6986 AMS-SNP37 7650388 841 7000 AMS-SNP38 7650454 907 7066 AMS-SNP39 7650501 954 7113 AMS-SNP40 7650580 1033 7192 AMS-SNP41 7650592 1045 7204 AMS-SNP42 7650715 1168 7327 AMS-SNP43 7650778 1231 7390 AMS-SNP44 7650850 1303 7462 AMS-SNP45 7650906 1359 7518 AMS-SNP46 7650907 1360 7519 AMS-SNP47 7650936 1389 7548 AMS-SNP48 7650945 1398 7557 AMS-SNP49 7650946 1399 7558 AMS-SNP50 7650950 1403 7562 AMS-SNP51 7650999 1452 7612 AMS-SNP52 7651063 1516 7676 AMS-SNP53 7651128 1581 7741 AMS-SNP54 7651177 1630 7791 AMS-SNP55 7651183 1636 7797 AMS-SNP56 7651192 1645 7806 AMS-SNP57 7651194 1647 7808 AMS-SNP58 7651196 1649 7810 AMS-SNP59 7651202 1655 7816 AMS-SNP60 7651221 1674 7835 AMS-SNP61 7651232 1685 7846 AMS-SNP62 7651233 1686 7847 AMS-SNP63 7651246 1699 7860 AMS-SNP64 7651291 1744 7905 AMS-SNP65 7651297 1750 7911 AMS-SNP66 7651304 1757 7918 AMS-SNP67 7651333 1786 7947 AMS-SNP68 7651646 2099 8261 AMS-SNP69 7651658 2111 8273 AMS-SNP70 7651815 2268 8430 AMS-SNP71 7651912 2365 8527 AMS-SNP72 7651915 2368 8530 AMS-SNP73 7651928 2381 8543 AMS-SNP74 7652112 2565 8728 AMS-SNP75 7652281 2734 8898 AMS-SNP76 7652295 2748 8912 AMS-SNP77 7652299 2752 8916 AMS-SNP78 7652316 2769 8933 AMS-SNP79 7652401 2854 9018 AMS-SNP80 7652406 2859 9023 AMS-SNP81 7652431 2884 9050 AMS-SNP82 7652443 2896 9062 AMS-SNP83 7652490 2943 9109 AMS-SNP84 7652506 2959 9126 AMS-SNP85 7652541 2994 9173 AMS-SNP86 7652546 2999 9178 AMS-SNP87 7652550 3003 9182 AMS-SNP88 7652560 3013 9200 AMS-SNP89 7652579 3032 9237 AMS-SNP90 7652580 3033 9238 AMS-SNP91 7652587 3040 9245 AMS-SNP92 7652666 3119 9324 AMS-SNP93 7652724 3177 9383 bHLH91 (start) 65763028 1 bHLH91-SNPl 65763078 50 10856 bHLH91-SNP2 65763196 168 10974 bHLH91-SNP3 65763208 180 10986 bHLH91-SNP4 65763240 212 11018 bHLH91-SNP5 65763246 218 11024 bHLH91-SNP6 65763300 272 11078 bHLH91-SNP7 65763434 406 11212 bHLH91-SNP8 65763463 435 11241 bHLH91-SNP9 65763792 764 11570 bHLH91-SNP10 65764200 1172 11981 bHLH91-SNPll 65764407 1379 12188 bHLH91-SNP12 65764533 1505 12314 bHLH91-SNP13 65765419 2391 13207 bHLH91-SNP14 65765509 2481 13297 bHLH91-SNP15 65765549 2521 13337 bHLH91-SNP16 65765714 2686 13502 TM6 (start) 85338862 1 TM6-SNP1 85338801 -61 14409 TM6-SNP2 85338904 42 14537 TM6-SNP3 85338905 43 14538 TM6-SNP4 85338913 51 14546 TM6-SNP5 85338928 66 14597 TM6-SNP6 85338978 116 14869 TM6-SNP7 85339009 147 14963 TM6-SNP8 85339010 148 14964 TM6-SNP9 85339012 150 14966 TM6-SNP10 85339018 156 14985 TM6-SNP11 85339020 158 15012 TM6-SNP12 85339023 161 15015 TM6-SNP13 85339035 173 15027 TM6-SNP14 85339134 272 15341 TM6-SNP15 85339521 659 16471 TM6-SNP16 85339545 683 16553 TM6-SNP17 85339578 716 16606 TM6-SNP18 85339728 866 17184 TM6-SNP19 85345536 6674 26531 TM6-SNP20 85345549 6687 26544 TM6-SNP21 85345597 6735 26619 TM6-SNP22 85345598 6736 26620 TM6-SNP23 85345622 6760 26648 TM6-SNP24 85345699 6837 26748 TM6-SNP25 85345701 6839 26750 TM6-SNP26 85345753 6891 26825 TM6-SNP27 85345760 6898 26832 TM6-SNP28 85345761 6899 26833 TM6-SNP29 85345766 6904 26838 TM6-SNP30 85345770 6908 26846 TM6-SNP31 85345779 6917 26869 TM6-SNP32 85345781 6919 26871 TM6-SNP33 85345783 6921 26873 TM6-SNP34 85345787 6925 26877 TM6-SNP35 85345789 6927 26880 TM6-SNP36 85345791 6929 26882 TM6-SNP37 85345792 6930 26883 TM6-SNP38 85345793 6931 26884 TM6-SNP39 85345794 6932 26885 TM6-SNP40 85345795 6933 26886 TM6-SNP41 85345796 6934 26887 TM6-SNP42 85345798 6936 26889 TM6-SNP43 85345801 6939 26892 TM6-SNP44 85345811 6949 26902 TM6-SNP45 85345818 6956 26909 TM6-SNP46 85345832 6970 26924 TM6-SNP47 85345840 6978 26937 TM6-SNP48 85345865 7003 26971 TM6-SNP49 85345882 7020 27005 TM6-SNP50 85345903 7041 27026 TM6-SNP51 85345915 7053 27047 TM6-SNP52 85345920 7058 27052 TM6-SNP53 85345926 7064 27058 TM6-SNP54 85345934 7072 27066 TM6-SNP55 85345935 7073 27067 TM6-SNP56 85345943 7081 27075 TM6-SNP57 85345944 7082 27076 TM6-SNP58 85345946 7084 27081 TM6-SNP59 85345947 7085 27082 TM6-SNP60 85345948 7086 27083 TM6-SNP61 85345949 7087 27084 TM6-SNP62 85345950 7088 27085 TM6-SNP63 85345951 7089 27086 TM6-SNP64 85345952 7090 27087 TM6-SNP65 85345953 7091 27088 TM6-SNP66 85345954 7092 27089 TM6-SNP67 85345955 7093 27090 TM6-SNP68 85345957 7095 27110 TM6-SNP69 85345959 7097 27112 TM6-SNP70 85345960 7098 27113 TM6-SNP71 85345964 7102 27117 TM6-SNP72 85345979 7117 27132 STP6 (start) 829664 1 STP6-SNP1 829568 -96 10799 STP6-SNP2 829577 -87 10808 STP6-SNP3 829587 -77 10818 STP6-SNP4 829608 -56 10839 STP6-SNP5 829622 -42 10853 STP6-SNP6 829623 -41 10854 STP6-SNP7 829624 -40 10855 STP6-SNP8 829648 -16 10879 STP6-SNP9 829669 5 10900 STP6-SNP10 829676 12 10909 STP6-SNP11 829677 13 10910 STP6-SNP12 829701 37 10944 STP6-SNP13 829705 41 10948 STP6-SNP14 829707 43 10950 STP6-SNP15 829731 67 10975 STP6-SNP16 829789 125 11033 STP6-SNP17 829801 137 11045 STP6-SNP18 829828 164 11072 STP6-SNP19 829840 176 11084 STP6-SNP20 829846 182 11090 STP6-SNP21 829858 194 11102 STP6-SNP22 829864 200 11108 STP6-SNP23 829873 209 11117 STP6-SNP24 829978 314 11223 STP6-SNP25 829987 323 11232 STP6-SNP26 829997 333 11242 STP6-SNP27 830002 338 11247 STP6-SNP28 830014 350 11259 STP6-SNP29 830083 419 11328 STP6-SNP30 830116 452 11361 STP6-SNP31 830146 482 11391 STP6-SNP32 830179 515 11424 STP6-SNP33 830182 518 11427 STP6-SNP34 830191 527 11436 STP6-SNP35 830203 539 11448 STP6-SNP36 830260 596 11505 STP6-SNP37 830281 617 11526 STP6-SNP38 831101 1437 13178 STP6-SNP39 831108 1444 13185 STP6-SNP40 831254 1590 13331 STP6-SNP41 831285 1621 13362 STP6-SNP42 831314 1650 13391 STP6-SNP43 831320 1656 13397 STP6-SNP44 831347 1683 13424 STP6-SNP45 831404 1740 13481 STP6-SNP46 831446 1782 13523 STP6-SNP47 831464 1800 13541 STP6-SNP48 831480 1816 13557 STP6-SNP49 831500 1836 13577 STP6-SNP50 831506 1842 13583 STP6-SNP51 831507 1843 13584 STP6-SNP52 831509 1845 13586 STP6-SNP53 831545 1881 13622 STP6-SNP54 831563 1899 13640 STP6-SNP55 831566 1902 13643 STP6-SNP56 831575 1911 13652 STP6-SNP57 831587 1923 13664 STP6-SNP58 831614 1950 13691 STP6-SNP59 831650 1986 13727 STP6-SNP60 831674 2010 13751 STP6-SNP61 831683 2019 13760 STP6-SNP62 831690 2026 13767 STP6-SNP63 831692 2028 13769 STP6-SNP64 831704 2040 13781 STP6-SNP65 831705 2041 13782 STP6-SNP66 831716 2052 13793 STP6-SNP67 831817 2153 13975 STP6-SNP68 831836 2172 13994 STP6-SNP69 831838 2174 13996 STP6-SNP70 831859 2195 14017 STP6-SNP71 831868 2204 14026 STP6-SNP72 831922 2258 14080 STP6-SNP73 831934 2270 14092 STP6-SNP74 831937 2273 14095 STP6-SNP75 831961 2297 14119 STP6-SNP76 831964 2300 14122 STP6-SNP77 832009 2345 14167 STP6-SNP78 832013 2349 14171 STP6-SNP79 832069 2405 14227 STP6-SNP80 832174 2510 14332 STP6-SNP81 832221 2557 14379 STP6-SNP82 832224 2560 14382 STP6-SNP83 832225 2561 14383 STP6-SNP84 832237 2573 14395 STP6-SNP85 832248 2584 14407 STP6-SNP86 832261 2597 14420 STP6-SNP87 832262 2598 14421 STP6-SNP88 832284 2620 14445 STP6-SNP89 832289 2625 14450 STP6-SNP90 832293 2629 14454 STP6-SNP91 832294 2630 14455 STP6-SNP92 832319 2655 14480 STP6-SNP93 832326 2662 14487 STP6-SNP94 832338 2674 14499 STP6-SNP95 832397 2733 14560 STP6-SNP96 832399 2735 14562 STP6-SNP97 832400 2736 14563 STP6-SNP98 832436 2772 14603 STP6-SNP99 832437 2773 14604 STP6-SNP100 832438 2774 14605 DYT1 (start) 93247539 DYT1-SNP1 93247305 -234 8290 DYT1-SNP2 93247327 -212 8312 DYT1-SNP3 93247328 -211 8313 DYT1-SNP4 93247337 -202 8322 DYT1-SNP5 93247342 -197 8327 DYT1-SNP6 93247347 -192 8332 DYT1-SNP7 93247358 -181 8343 DYT1-SNP8 93247390 -149 8375 DYT1-SNP9 93247447 -92 8432 DYT1-SNP10 93247455 -84 8440 DYT1-SNP11 93247474 -65 8459 DYT1-SNP12 93247512 -27 8497 DYT1-SNP13 93247519 -20 8504 DYT1-SNP14 93247545 6 8530 DYT1-SNP15 93247767 228 8752 DYT1-SNP16 93247785 246 8770 DYT1-SNP17 93247833 294 8818 DYT1-SNP18 93247847 308 8832 DYT1-SNP19 93247868 329 8853 DYT1-SNP20 93247981 442 8993 DYT1-SNP21 93247995 456 9007 DYT1-SNP22 93248028 489 9040 DYT1-SNP23 93248075 536 9087 DYT1-SNP24 93248107 568 9119 DYT1-SNP25 93248266 727 9278 DYT1-SNP26 93248281 742 9293 DYT1-SNP27 93248326 787 9338 DYT1-SNP28 93248365 826 9377 DYT1-SNP29 93248374 835 9386 DYT1-SNP30 93248381 842 9393 DYT1-SNP31 93248474 935 9486 DYT1-SNP32 93248532 993 9544 DYT1-SNP33 93248575 1036 9597 MYB35-15 (start) 60560650 MYB35-15-SNP1 60560334 -316 10354 MYB35-15-SNP2 60560335 -315 10355 MYB35-15-SNP3 60560347 -303 10367 MYB35-15-SNP4 60560348 -302 10368 MYB35-15-SNP5 60560349 -301 10369 MYB35-15-SNP6 60560350 -300 10370 MYB35-15-SNP7 60560354 -296 10374 MYB35-15-SNP8 60560372 -278 10392 MYB35-15-SNP9 60560373 -277 10393 MYB35-15-SNP10 60560377 -273 10397 MYB35-15-SNP11 60560378 -272 10398 MYB35-15-SNP12 60560379 -271 10399 MYB35-15-SNP13 60560390 -260 10410 MYB35-15-SNP14 60560392 -258 10412 MYB35-15-SNP15 60560393 -257 10413 MYB35-15-SNP16 60560394 -256 10414 MYB35-15-SNP17 60560395 -255 10415 MYB35-15-SNP18 60560400 -250 10420 MYB35-15-SNP19 60560401 -249 10421 MYB35-15-SNP20 60560404 -246 10424 MYB35-15-SNP21 60560407 -243 10481 MYB35-15-SNP22 60560408 -242 10482 MYB35-15-SNP23 60560409 -241 10483 MYB35-15-SNP24 60560418 -232 10492 MYB35-15-SNP25 60560421 -229 10495 MYB35-15-SNP26 60560422 -228 10496 MYB35-15-SNP27 60560423 -227 10497 MYB35-15-SNP28 60560425 -225 10499 MYB35-15-SNP29 60560433 -217 10507 MYB35-15-SNP30 60560442 -208 10517 MYB35-15-SNP31 60560445 -205 10520 MYB35-15-SNP32 60560449 -201 10524 MYB35-15-SNP33 60560450 -200 10525 MYB35-15-SNP34 60560452 -198 10528 MYB35-15-SNP35 60560464 -186 10540 MYB35-15-SNP36 60560481 -169 10557 MYB35-15-SNP37 60560484 -166 10560 MYB35-15-SNP38 60560485 -165 10561 MYB35-15-SNP39 60560495 -155 10571 MYB35-15-SNP40 60560496 -154 10572 MYB35-15-SNP41 60560497 -153 10573 MYB35-15-SNP42 60560499 -151 10575 MYB35-15-SNP43 60560500 -150 10576 MYB35-15-SNP44 60560501 -149 10577 MYB35-15-SNP45 60560504 -146 10595 MYB35-15-SNP46 60560505 -145 10596 MYB35-15-SNP47 60560511 -139 10602 MYB35-15-SNP48 60560518 -132 10609 MYB35-15-SNP49 60560520 -130 10611 MYB35-15-SNP50 60560522 -128 10613 MYB35-15-SNP51 60560524 -126 10615 MYB35-15-SNP52 60560528 -122 10619 MYB35-15-SNP53 60560614 -36 10729 MYB35-15-SNP54 60560669 19 10812 MYB35-15-SNP55 60560735 85 10878 MYB35-15-SNP56 60560771 121 10917 MYB35-15-SNP57 60560887 237 11040 MYB35-15-SNP58 60560902 252 11055 MYB35-15-SNP59 60560923 273 11076 MYB35-15-SNP60 60560932 282 11085 MYB35-15-SNP61 60560944 294 11097 MYB35-15-SNP62 60560989 339 11142 MYB35-15-SNP63 60561213 563 11391 MYB35-15-SNP64 60561249 599 11427 MYB35-15-SNP65 60561315 665 11521 MYB35-15-SNP66 60561316 666 11522 MYB35-15-SNP67 60561347 697 11554 MYB35-15-SNP68 60561350 700 11557 MYB35-15-SNP69 60561398 748 11605 MYB35-15-SNP70 60561422 772 11629 MYB35-15-SNP71 60561431 781 11638 MYB35-15-SNP72 60561459 809 11670 MYB35-15-SNP73 60561464 814 11675 MYB35-15-SNP74 60561495 845 11706 MYB35-15-SNP75 60561514 864 11725 MYB35-15-SNP76 60561515 865 11726 MYB35-15-SNP77 60561532 882 11746 MYB35-15-SNP78 60561536 886 11750 MYB35-15-SNP79 60561537 887 11751 MYB35-15-SNP80 60561540 890 11754 MYB35-15-SNP81 60561542 892 11756 MYB35-15-SNP82 60561548 898 11762 MYB35-15-SNP83 60561556 906 11770 MYB35-15-SNP84 60561576 926 11790 MYB35-15-SNP85 60561588 938 11802 MYB35-15-SNP86 60561602 952 11849 MYB35-15-SNP87 60561607 957 11854 MYB35-15-SNP88 60561608 958 11855 MYB35-15-SNP89 60561609 959 11856 MYB35-15-SNP90 60561667 1017 11933 MYB35-15-SNP91 60561742 1092 12026 MYB35-15-SNP92 60561747 1097 12031 MYB35-15-SNP93 60561750 1100 12034 MYB35-15-SNP94 60561764 1114 12048 MYB35-15-SNP95 60561767 1117 12051 MYB35-15-SNP96 60561779 1129 12063 MYB35-15-SNP97 60561795 1145 12079 MYB35-15-SNP98 60561819 1169 12103 MYB35-15-SNP99 60561856 1206 12140 MYB35-15-SNP100 60561879 1229 12163 MYB35-15-SNP101 60561891 1241 12180 MYB35-15-SNP102 60561920 1270 12209 MYB35-15-SNP103 60561935 1285 12224 MYB35-15-SNP104 60561941 1291 12230 MYB35-15-SNP105 60561956 1306 12252 MYB35-15-SNP106 60561967 1317 12263 MYB35-15-SNP107 60561991 1341 12295 MYB35-15-SNP108 60561999 1349 12303 MYB35-15-SNP109 60562024 1374 12330 MYB35-15-SNP110 60562027 1377 12333 MYB35-15-SNP111 60562030 1380 12336 MYB35-15-SNP112 60562068 1418 12383 MYB35-15-SNP113 60562088 1438 12424 MYB35-15-SNP114 60562099 1449 12435 MYB35-15-SNP115 60562106 1456 12442 MYB35-15-SNP116 60562119 1469 12455 MYB35-16 (start) 83254697 MYB35-16-SNP1 83254499 -198 7627 MYB35-16-SNP2 83254539 -158 8229 MYB35-16-SNP3 83254560 -137 11825 MYB35-16-SNP4 83254561 -136 11826 MYB35-16-SNP5 83254562 -135 11827 MYB35-16-SNP6 83254564 -133 11829 MYB35-16-SNP7 83254565 -132 11830 MYB35-16-SNP8 83254568 -129 11833 MYB35-16-SNP9 83254569 -128 11834 MYB35-16-SNP10 83254570 -127 11835 MYB35-16-SNP11 83254572 -125 11837 MYB35-16-SNP12 83254573 -124 11838 MYB35-16-SNP13 83254576 -121 11910 MYB35-16-SNP14 83254583 -114 11917 MYB35-16-SNP15 83254585 -112 11924 MYB35-16-SNP16 83254614 -83 11953 MYB35-16-SNP17 83254623 -74 11962 MYB35-16-SNP18 83254628 -69 11967 MYB35-16-SNP19 83254648 -49 11987 MYB35-16-SNP20 83254651 -46 11990 MYB35-16-SNP21 83254656 -41 11995 MYB35-16-SNP22 83254682 -15 12022 MYB35-16-SNP23 83254685 -12 12025 MYB35-16-SNP24 83254686 -11 12026 MYB35-16-SNP25 83254687 -10 12027 MYB35-16-SNP26 83254691 -6 12031 MYB35-16-SNP27 83254795 98 12135 MYB35-16-SNP28 83254874 177 12214 MYB35-16-SNP29 83254933 236 12273 MYB35-16-SNP30 83254957 260 12297 MYB35-16-SNP31 83254984 287 12324 MYB35-16-SNP32 83254986 289 12326 MYB35-16-SNP33 83255046 349 12386 MYB35-16-SNP34 83255067 370 12407 MYB35-16-SNP35 83255077 380 12417 MYB35-16-SNP36 83255115 418 12455 MYB35-16-SNP37 83255174 477 12514 MYB35-16-SNP38 83255236 539 12576 MYB35-16-SNP39 83255238 541 12578 MYB35-16-SNP40 83255254 557 12594 MYB35-16-SNP41 83255262 565 12602 MYB35-16-SNP42 83255314 617 12657 MYB35-16-SNP43 83255389 692 12732 MYB35-16-SNP44 83255458 761 12801 MYB35-16-SNP45 83256035 1338 13491 MYB35-16-SNP46 83256399 1702 13901 MYB35-16-SNP47 83256400 1703 13902 MYB35-16-SNP48 83256440 1743 13942 MYB35-16-SNP49 83256442 1745 13944 MYB35-16-SNP50 83256462 1765 13965 MYB35-16-SNP51 83256463 1766 13966 MYB35-16-SNP52 83256472 1775 13975 MYB35-16-SNP53 83256488 1791 13991 MYB35-16-SNP54 83256493 1796 13996 MYB35-16-SNP55 83256496 1799 13999 MYB35-16-SNP56 83256503 1806 14006 MYB35-16-SNP57 83256520 1823 14023 MYB35-16-SNP58 83256521 1824 14024 MYB35-16-SNP59 83256527 1830 14030 MYB35-16-SNP60 83256528 1831 14031 MYB35-16-SNP61 83256529 1832 14032 MYB35-16-SNP62 83256532 1835 14037 MYB35-16-SNP63 83256533 1836 14038 MYB35-16-SNP64 83256559 1862 14065 MYB35-16-SNP65 83256610 1913 14116 MYB35-16-SNP66 83256621 1924 14127 MYB35-16-SNP67 83256622 1925 14128 MYB35-16-SNP68 83256623 1926 14129 MYB35-16-SNP69 83256646 1949 14152 MYB35-16-SNP70 83256655 1958 14161 MYB35-16-SNP71 83256673 1976 14179 MYB35-16-SNP72 83256698 2001 14206 MYB35-16-SNP73 83256722 2025 14230 MYB80 (start) 377128 MYB80-SNP1 377176 48 5515 MYB80-SNP2 377202 74 5541 MYB80-SNP3 377203 75 5542 MYB80-SNP4 377218 90 5557 MYB80-SNP5 377219 91 5558 MYB80-SNP6 377320 192 5661 MYB80-SNP7 377342 214 5683 MYB80-SNP8 377406 278 5748 MYB80-SNP9 377541 413 5887 MYB80-SNP10 377552 424 5898 MYB80-SNP11 377582 454 5929 MYB80-SNP12 377624 496 6992 MYB80-SNP13 377818 690 7195 MYB80-SNP14 377947 819 7324 MYB80-SNP15 377959 831 7336 MYB80-SNP16 377975 847 7352 MYB80-SNP17 377976 848 7357 MYB80-SNP18 377977 849 7358 MYB80-SNP19 377996 868 7377 MYB80-SNP20 378019 891 7400 MYB80-SNP21 378029 901 7410 MYB80-SNP22 378086 958 7477 MYB80-SNP23 378134 1006 7525 MYB80-SNP24 378206 1078 7597 MYB80-SNP25 378319 1191 7710 MYB80-SNP26 378379 1251 7770 MYB80-SNP27 378598 1470 7999 MYB80-SNP28 378673 1545 8074 MYB80-SNP29 378715 1587 8116 MYB80-SNP30 378742 1614 8143 MYB80-SNP31 378754 1626 8155 MYB80-SNP32 378796 1668 8197 MYB80-SNP33 378823 1695 8224 MYB80-SNP34 378829 1701 8230 MYB80-SNP35 378853 1725 8263 MYB80-SNP36 378877 1749 8287 MYB80-SNP37 378886 1758 8296 MYB80-SNP38 378887 1759 8297 MYB80-SNP39 378919 1791 8329 MYB80-SNP40 378988 1860 8398 MYB80-SNP41 379012 1884 8422 MYB80-SNP42 379057 1929 8467 MYB80-SNP43 379066 1938 8476 MYB80-SNP44 379102 1974 8512 MYB80-SNP45 379269 2141 8683 MYB80-SNP46 379300 2172 8714 We also conducted a population structure analysis to avoid potential issues in RNAi design due to specific clustering patterns in the population. The raw VCF file was processed to retain high quality variations for genetic structure analysis as follows: (1) the variants were 5 filtered to remove any INDELs from the dataset; (2) the resulting variants were filtered to retain SNPs present in at least 90% of the samples and have minimum read depth of 5; (3) the remaining variants were further filtered using the minor allele frequency of 0.95. The final variants were used to perform a principal component (PC) analysis in the Tassel software (Bradbury et al. 2007). The first three PCs were plotted to visualize the genetic structure in the 10 Cannabis population. This analysis revealed that a single accession, ‘Malawi Gold,’ was completely distinct from the remaining samples in the LeafWorks database. The remaining cultivars showed a random distribution pattern across the three principal components. A similar population structure analysis was conducted using the SNPs within a common genotype block spanning each gene of interest. The common genotype blocks were defined from the genome- 15 wide SNP dataset using plink software (Purcell et al. 2007). The gene-specific PC plots mostly showed random distribution of samples across the three PCs, indicating that most of the Cannabis accessions in the LeafWorks database used in this analysis were suitable for RNAi experiments. A lack of distinct clustering of Cannabis accessions indicates that these Cannabis accessions lack specific population structure, which makes it possible to choose any Cannabis accession as a representative to do RNAi. Example 3—RNAi dsRNA Construction dsRNA Design'. The software, pssRNAit (Ahmed et al. 2020), was used to design the dsRNAs for each gene target. psRNATarget (Dai and Zhao 2011) was also used to look for off-target hits against the Cannabis transcriptome as well as look for off-target hits against Homo sapiens (i.e., humans). When evaluating different dsRNAs for each gene target, we assessed the potential number of siRNAs generated from a dsRNA molecule, total number of off-target hits, average siRNA per off target, average ‘expect’ score for off target hits, average siRNA efficiency, average target accessibility, and average RISC binding differential to decide which ones to pursue. Further, we investigated human off target hits and, if any dsRNA created multiple potential siRNAs that target human genes (i.e., that had a greater likelihood of eliciting a RNAi response for a human gene), these were removed as potential candidates. Each gene had a single annotated transcript except for LOCI 15713370, which has five annotated transcripts in the cslO genome. During dsRNA design, we confirmed that siRNAs generated from potential dsRNA would bind to all annotated transcripts for target loci. Further, all annotated transcripts were taken into account in RTqPCR marker design to avoid confounding results. Information for dsRNA design can be found in Table 5. dsRNA for each of the four genes was synthesized by Creative Biogene Inc (Shirley, NY, USA). Product was synthesized using standard in vitro transcription (IVT) and lithium chloride for precipitation. Product passed quality inspection with clear appearance free from foreign particles, free of DNA, protein and nucleotide, OD260 / 280 at 2.0-2.1, and gel agarose band clean at the correct size. Final concentration was measured with a Nanodrop device (Thermo Fisher Scientific, Waltham, MA, USA). Schematics showing the design of the dsRNA molecules to each of the four genes are shown in FIG. 1A (AMS); FIG. IB (bHLH91); FIG. IC (TM6); FIG. ID (STP6). Cl G) Table 5: Gene dsRNA and RTqPCR information, including gene name, Gene ID, length, primer / probe sequences, amplicon size, predicted number of siRNAs from dsRNA Target Gene Name Gene ID Locus ID Gene Length Forward Primer Sequence Reverse Primer Sequence Probe Sequence Amplicon Product Size dsRNA size Predicted number of siRNA Experimental bHLH91 LOCI 15713370 XM 030641854 2625 bp GCTTGATCTTCGCCAT GT (SEQ ID NO: 18) GCTATTTGTAGGTGGA AGTGGAA (SEQ ID NO: 19) TGTGGGAGACACTTAC AGCTTC (SEQ ID NO:20) 285 197bp 14 Experimental TM6 LOCI 15714657 XM 030643408 7003 bp CCAAAGGTGTTGATCT GTGG(SEQ ID NO:21) CTGTCTGATCTGTGTC CTCA (SEQ ID NO:22) GCAACAAACCTTGCGC CAACT (SEQ ID NO:23 101 140bp 13 Experimental AMS LOCI 15706375 XM 030634011 2607 bp TGCATGGAAGGACTG GAGAA (SEQ ID NO:24) TAGTGGGCTCGAAAG GATGG (SEQ ID NO:25) TCAGTCCCAGCACAAC AGCA (SEQ ID NO:26) 245 143bp 4 Experimental STP6 LOC115714521 XM 030643251 2658 bp ATCGGTTTCGGGAGTG AT (SEQ ID NO:27) CGGCATTGTACTTGTA GCTG(SEQ ID NO:28) GCATCCCTTTTGTCATC G (SEQ ID NO:29) 283 200bp 6 Control EFl LOCI 15725432 JP452083 2465 bp AGAAGGCTCCTCGTTG TCTT (SEQ ID NO:30) CACCCTTCCAAGAGCT CTACA (SEQ ID NO:31) TGCACGGATCAGTTTG CGTTG (SEQ ID NO:32) 110 NA NA WO 2025 / 221819                                   PCT / US2025 / 024812 Example 4—Gene Expression Testing RTqPCR Design'. We used FASTA and CDS sequences for each gene target for RTqPCR marker design. Markers were designed to span exons, thus gene expression evaluation was robust to DNA contamination and would ensure amplification of mRNA transcripts, not genomic DNA. Markers were designed using Primer3 v4.1.0 (Untergasser et al. 2012) and Geneious 2020.2.5 (Kearse et al. 2012). For a gene expression control reference marker, we selected EFlalpha for Cannabis based on work from Guo et al. (2018). We designed a probe to match primers published in Guo et al. (2018) using Primer3 v4.1.0 (Untergasser et al. 2012) (see Table 5)- RTqPCR testing and validation. Each marker was evaluated for PCR efficiency and expression levels of mRNA extracted from either male flowers or leaves. Note that the selected target genes are all putatively involved in hermaphroditic or male flower formation and / or pollen formation and, therefore, expression is expected to be minimal in RNA extracted from leaf material. Gene expression is expected to be higher in the flowers given their function. Leaf expression data was utilized as a control to show expression changes. The results are outlined below in Table 6. Table 6. Gene PCR efficiency information. Marker PCR efficiency % amplification in male flower RNA % amplification in leaf RNA AMS 89% 100 0 bHLH91 88% 100 100* STP6 99% 50 0 TM6 99% 100 0 EFl 97.00% 100 100 *Note that Cq values for bHLH91 leaf RNA were always substantially lower than Cq values for male flower RNA. Example 5—dsRNA Application Plant Material Used. Cannabis cultivars used for each dsRNA application are listed in Table 7. The cultivar ‘Original Bacio’ was used to test the silencing effect of bHLH91 anti AMS in Cocktail 1. The cultivars ‘Original Bacio,’ ‘Velvet Grapes’ and ‘ Soap’ were used to test the silencing effect of TM6 and STP6 in Cocktail 2. These cultivars come from different lineages and were chosen to showcase the effectiveness in silencing across diverse genetic backgrounds. Plant Cultivation Conditions. Young plants from clone cuttings from each cultivar (Table 7) were grown in replicate (i.e., 2 clones), potted in FoxFarm Happy Frog Potting Soil (Samoa, CA, USA) in 2-gallon pots. Plants were grown in MyHighGrow 3x3m grow tents (Perth Amboy, NJ, USA), each tent representing a different experimental treatment. The lights were set to create a 18 hour photoperiod during vegetative growth and a 12 hour photoperiod during flowering (see STS section below). Plants were fertilized with House & Garden Soil A + B Nutrients (Seacliff Park, South Australia) and General Hydroponics CALIMAGIC™ calcium-magnesium supplement (Santa Rosa, CA, USA) according to the manufacturer's instructions. Pests and pathogens were checked regularly, with Trifecta Crop Control (Williamsport, PA, USA) applied as needed. Cl <0 Table 7: Cultivar Information for Cultivars used in RNAi Application Experiments Cultivar Cross Flowering time (days) THC average Aroma Appearance Dominant terpene Minor terpenes Treatments Used Soap Kush Mints x Animal Mints 53-64 30% Rose, effervescent, vanilla Dark semi dense green and purple buds and amber pistils Caryophyllene Limonene, linalool Cocktail 2 Velvet Grapes Strawberry' Cough x Grape Ape 56-60 20-23% Spicy, herbal, berry, grape Deep purple buds + thin orange hairs Ocimene Eucalyptol, sabincnc Cocktail 2 Original Bacio Sunset Sherbert x Thin Mint GSC 63-65 23% Citrus, pungent, gas Light to dark purple buds + amber pistils Camphene Caryophyllene Cocktail 1, Cocktail 2 WO 2025 / 221819                                   PCT / US2025 / 024812 Hermaphrodite Creation'. To test if silencing of these genes would result in aborted pollen or not (i.e., viable), male flower production was induced in all experimental plant tents (FIG. 2). Female plants in which male flower production was required were sprayed with a Silver thiosulfate (STS) solution. STS induces male flower production via an endogenous hermaphroditic mechanism in Cannabis (Lubell and Brand 2018). STS solution was made by adding 3.79g NaS2O3 (STS) to 160 mL H2O, adding 0.69 g AgNOa to 40 mL H2O, mixing well and then mixing together for a total volume of 200 mL. The 200 mL solution was then diluted with 3 L H2O for a total volume of 3200 mL. STS spray was applied uniformly to each plant until the entire plant surface was covered and the solution was dripping in excess. The daily light cycle was changed to a 12-hour photoperiod on the day of the first application. Three additional STS spray treatments (five days, 11 days and 17 days) were applied after the first application to ensure hermaphroditism would occur. dsRNA Spray Creation. dsRNA were manufactured by Creative Biogene Inc (Shirley, NY, USA) and were validated using gel electrophoresis to verify desired fragment size. To create the spray solution, RNase activity was prevented by baking instruments at 150°C and covered with foil for three hours to maintain sterilization prior to use. The ventilation hood in which work was performed was sterilized by wiping down the surface and pipettes with RNase Away and applying UV lights prior to working to inactivate any RNase. Only RNase-free pipet tips were used. In one of the many studies to evaluate nanoparticles for dsRNA efficacy, Xu et al. (2023) found that chitosan quaternary ammonium salt (CQAS) was the most effective at cellular uptake among all compared nanoparticle-dsRNAs complexes and naked-dsRNA. Further, when multiple types of nanoparticles are sprayed, CQAS exhibited consistent uptake in multiple plant species such as tobacco, tomato, and pepper. Thus, we used CQAS as a nanoparticle to aid dsRNA uptake. To make the CQAS solution, dsRNA was complexed with 0.02% CQAS using methods established by Xu et al. (2023) for RNAi application. 75% deacetylated CQAS was dissolved in 0.1 M sodium acetate buffer to produce a solution with a concentration of 0.02% (w / v). dsRNA was bound to 0.02% CQAS in 100 pL of Na2SO4 (0.25 mol / L). Immediately following one minute of incubation at 55°C, the solution was vigorously vortexed for 30 seconds to facilitate the formation of the dsRNA-CQAS complex. The mixture was allowed to sit for 30 minutes to ensure binding. A gel retention assay confirmed the complex creation was successful. Mixture was stored in a cool and dark place until use. Air exposure was minimized when loading and using the high-pressure air sprayer. dsRNA Spray Application-. Each spray application onto plants used an Iwata NEO CN Gravity Feed Dual Action Airbrush (Harrison, OH, USA) 5 mL high pressure sprayer airbrush (FIG. 2). One Cocktail 1 dsRNA spray applied 0.1 mg of dsRNA for bHLH91 and 0.1 mg of dsRNA for AMS in a 0.02% CQAS solution. One Cocktail 2 dsRNA spray applied 0.1 mg of dsRNA for STP6 and 0.1 mg of dsRNA for TM6 in a 0.02% CQAS solution. Control sprays applied 0.02% CQAS solution alone without dsRNA present. Plants were sprayed with equal volume no matter the treatment. Spray heads were not shared across tent treatments to prevent contamination. FIG. 2 describes the experimental spray design for each cocktail. Cocktail 1 Experiments: Cocktail 1 Experimental and Control sprays were applied 34 days after the final STS treatment when females were producing hermaphroditic flowers throughout the plant. Plants were sprayed twice per week for four weeks in both the experimental and control tent with their respective spray types (FIG. 2). Gene expression of Cocktail 1 as compared to gene expression in the control tent was measured seven days after the first spray. Pollen abortion vs non-abortion rates from Cocktail 1 as compared to the control tent were measured by sampling dehisced pollen eight days after the final spray. Cocktail 2 Experiments: The first dsRNA spray of Cocktail 2 occurred 34 days after the final STS application when females were producing hermaphroditic flowers throughout the plant. Control spray was applied three times before the dsRNA spray was applied once. Gene expression was measured three days before the application of Cocktail 2 dsRNA spray and six days after to see the effect of dsRNA on gene expression. Pollen abortion vs nonabortion rates from Cocktail 2 were measured by sampling dehisced pollen two days prior to Cocktail 2 application, then seven days after. Gene Expression Analysis'. RTqPCR was performed to evaluate the effect of dsRNA application on target gene expression. Flower material from three branches on each experimental plant was collected and immediately stored in RNAlater for processing at a later date. RNA was extracted using Qiagen RNeasy Plant Kit (Hilden, Germany) modified to extract RNA from tissue stored in RNAlater and including on-column DNase treatment. Next, RNA was quantified using Qubit RNA HS assays (Waltham, MA, USA) and all RNA concentrations were standardized to a similar value. A NEB Luna Universal Probe One-Step RTqPCR kit (Ipswich, MA, USA) was used to perform RTqPCR experiments following the protocol of 55°C 30-min reverse transcription, 95°C three min denaturation, and 40 cycles of 95°C 10 seconds to 65°C one min, where hydrolysis probes were used to measure transcript abundance. EFl-alpha was used as an internal reference control as this is described to be the best for measuring gene expression in Cannabis sativa (Guo et al. 2018). We calculated relative gene expression using the standard 2-AA Cq method (Livak and Schmittgen 2001). We performed statistical analysis using the log transformed data and used a paired one-tailed t-test (TM6 and STP6) due to the paired nature of the before and after treatments for dsRNA Cocktail 2 and a standard one-tailed t-test (AMS and bHLH9F) between the control and experimental treatments for dsRNA Cocktail 1, as was statistically appropriate. Pollen Abortion Rate Measurement Pollen abortion rate was assessed by measuring the proportion of aborted and non-aborted pollen based upon Wizenberg et al. (2022). Briefly, dehisced pollen was shaken into a 1.5 mL microcentrifuge tube. Tubes were kept open up to 1 hour to dry before sealing and storage at 4°C before processing. A modified Alexander stain was prepared to stain pollen and test for abortion rates, following the protocol in Wizenberg et al. (2022). The following were combined: 10 mL of 95% alcohol, 1 mL of diluted malachite green (1% solution in 95% alcohol). 54.5 mL of distilled water, 25 mL of glycerol, 5 mL of diluted acid fuchsin (1% solution in distilled water), 0.5 mL of diluted orange G (1% solution in distilled water), 4 mL of glacial acetic acid. To prepare samples for evaluation of abortion rates, each pollen sample tube was unsealed and a clean cotton swab was used to apply a small sample of pollen by streaking the swab on the center of a 75 mm x 25 mm glass microscope slide. Aliquots of 20 pL of the modified Alexander stain were pipetted directly onto the applied pollen sample. Next, the prepared slide was heated approximately 5-15 cm above a butane torch lighter for roughly 5 seconds then allowed to cool to room temperature. Lastly, a glass slide cover (25 mm x 25 mm) was placed on the cooled sample and incubated at room temperature for 24 hours. Pollen abortion rates were estimated by counting aborted (stained blue) and nonaborted (stained purple) pollen grains, up to 100 grains total. Non-shriveled pollen grains with dark, purple contents inside the grain indicate complete pollen viability (non-abortion). Pollen grains that are blue, which look fully or largely empty inside, are not viable (aborted). To this end, a light microscope (AmScope Bl00; Irvine, CA, USA) was used to perform vertical transects at lOx magnification, moving across the length and width of the 2 mm2 slide cover until approximately 100 grains were counted. The number of aborted and nonaborted grains were counted using two hand-held tally counters. Any burst pollen grains on each slide were noted but excluded from the total number of grains. Any clumped pollen grains were excluded from counts. The proportion of pollen that stained as aborted and nonaborted as percentages of the total sample were calculated. To evaluate pollen abortion percentage differences and changes in gene expression from treatment with Cocktail 1, a one-tailed t-test was performed on plants sprayed with Cocktail 1 relative to plants sprayed with the Control spray. To evaluate pollen abortion percentage change in abortion rates and changes in gene expression from treatment with Cocktail 2, a paired one-tail t-test was performed on plants before and after exposure to Cocktail 2. All analyses were performed in JMP vl7 (Cary, NC, USA). Example 6—Pollen Abortion Rate Results Both dsRNA cocktails, Cocktail 1 and Cocktail 2, demonstrated a strong negative effect on pollen viability with significantly higher pollen abortion percentages than the control samples after each cocktail application. No other visible changes were observed; plants in all treatments remained healthy. The application of Cocktail 1 (bHLH91 and AMS dsRNA) resulted in a 74.63% difference in aborted pollen, rising to 62.89% aborted pollen on average in the Experimental Treatment from 28.72% aborted pollen in the Control Treatment (FIGs. 3-6) (t-ratio = -2.485; P-value = 0.0323). For an example image of pollen in control conditions for Cocktail 1, see FIG. 3. For an example image of pollen after spraying Cocktail 1, see FIG. 4. FIG. 7 is a graphical representation of pollen abortion rates in the presence of Cocktail 1. Prior to spraying dsRNA Cocktail 2, the control conditions exhibited an average aborted pollen percentage of 39.36%, but, after dsRNA Cocktail 2 exposure, aborted pollen percentage increased to 65.45% on average (t-ratio = 2.250, P-value = 0.0271). Thus, Cocktail 2 demonstrated a percent increase of 66.29% in aborted pollen. For an example image of pollen in control conditions for Cocktail 2, see FIG. 5. For an example image of pollen after spraying Cocktail 2, see FIG. 6. FIG. 8 is a graphical representation of pollen abortion rates before and after exposure to Cocktail 2. Example 7—Gene Expression Results A significant decrease in mRNA transcript levels was observed for target genes relative to control plants sprayed with control solution (FIGs. 9-12). RTqPCR analysis revealed that the level of AMS mRNAs were significantly lower in dsRNA-0.2% CQAS treated plants relative to control (FIG. 9, P<0.01) and a similar pattern was observed for mRNA levels of bHLH91 relative to controls (FIG. 10, P<0.01). Similarly, before and after comparisons for gene expression showed a statistically significant reduction of mRNA levels for STP6 across all cultivars tested (FIG. 11, P<0.01) and in a single cultivar (‘Original Bacio’) when looking at TM6 gene expression before and after spraying (FIG. 12, P<0.05). Example 8—Conclusions Each of the four gene targets across Cocktails 1 and 2 demonstrated a statistically significant decrease in gene expression as compared to the control, and the application of the dsRNA resulted in statistically significantly higher abortion rates. Pollen abortion rates and, thus, pollen inviability increased by 29.6% from Cocktail 1 and 66.92% from Cocktail 2 on average. While gene expression of TM6 was statistically significantly reduced only in ‘Original Bacio’, gene expression oiSTP6 was statistically significantly reduced across all cultivars. Concentration, timing and method of application of these dsRNA can alter the effectiveness of the gene silencing and phenotypic expression. With the demonstration that these genes directly inhibit viable pollen formation, the silencing, eradication or manipulation of these genes will hinder or eliminate male flower reproduction in Cannabis. The spray method is effective at significantly reducing the impact of male pollen in Cannabis. Example 9—Gene and Protein Sequences and Description of Variation Among Almost 200 Cannabis Accessions Gene name: AMS CBDRx assembly gene ID: LOCI 15706375 Number of Isoforms: GCF_029168945.1 6 Number of Indels: 4 Number of Exon SNPs: 100 Description of variation: many individuals have large ~2-3 kb insertions upstream and downstream of the start site for the AMS coding sequence. The AMS gene ends earlier for the CBDRx assemblies; exon 9, which is around -450 bp, is completely missing. In other individuals, exon 9 has one or more small -15 bp insertions. >AMS nucleic acid sequence from CBDRx (SEQ ID NO:1) T C AAC C AC AC T GT T AC AAGAAGT T C C AGC AG C AGT AAAAAGAT T AGAAAAAT AAT C AG C AAT AAT CAC AT T C AG ACTAGAATCCCAATCCTTCTAAACACCATTCGGGTCTTGTAATCTCAGAATTTGGTTACCTCGTTTCCTAGTGT TGGCTGAGGCATGGAAGTATTTGCTGTTTTTGTCTCCCGAATGAAGCCAATGTTGCTTGGATCTTTGCTTCCAG TAAAGTTCTTGCTGTGCCAAAATCTCAAAATAATTGTTTTTTTCTTCATCAAATTCAGCAGTAGCAGGATGGAT TGTTGAACTTTTCATGGCAGCCATTCGAGTTTTGCTTGTCTTCAGCCTGTGTTTGAAATTCCCAGTGAGTTCGA ACCCCCAAGCTCTCAAAGTGTTACTGCAATGATGAATTTTAACAGCTAGAGTAGAATTAGCATTAACCTCCCAG CAATTTTTCACAATTTGCTTACAATACGGCTCACGACTCCATGCGTTTTGATATTTAAAAAATTGAATGTGGTT GCCTCGGTTACTTACAGCAGGTTGCAGAAAAATGGGAGTATGGTCACTTGAAGAAAAATCACAGTTGGATAAAA TGGCATTGGGGAAAAGATCAAGCCAATGTTGGGTTACAAGAGCTTTATCTAACCTTCTTTCAACATGATGAGTA GTCCCTCTCCCTCGTTCCCATGTGAAGGCATAACCGTGAAGCTTCAAATCGACAAGACAGCAATGGCGAATGAC ATCTTGAAAGCCATTAATCAAGTATGAGGGGTAGGGTCGGCCTCCTTGTTTTTCATCTTGAGAAGCAATGTTAT TAAAATCGCCTATGATACACCAAGGCAAAGTCGAGCAAGGGGCAATGATCTTGAGCAGTTGCCAGGTACGGTCA CGAAGTGTTCGATTTGGTTCACCATAGAAGCCCGTGAGTCTCCATTGTGCAGAACCAGGAGAGTGAATTTCAAT GTCAATGTGGTTGTCCAAATATCCAAGCAGATGGGCATCATCAGTATTTTTCCAAAGAAGAGCAAGGCCACCTT TCCTCCCTTGGGCTTCAACAACAAAGCAACTATCAAAGCCCAACTTAGTTTTTATACGATCCCTAGCATCTTTT TTACACAAAGTCTCACATAGGAAAAGGAAATTGGGTTTCTTTTGGACACAAATGTCTGTTAGGAATTGAATAGC CCGTGGGTTCCCAAGCCCACGACAATTCCAACTAAGAGTATTCATGATGAGCGGTGGGCCTGAACACCAGAGCC CACCACAAAATCGTTTTTTGGCATCTCCTCAGTATTTGCAGGAATACCAATATTTATCTCATTAACTTGTTCTA TATCAACCATATTTGCCACATGTGAATTCAGTATGGACCCCTTCTCCCCACGTTTTCTTTTAAGGTCCAATATG GTAAGTTCATCATCAGACATAAAGGTAGAATCATTACCATTAATTACAATTGATGGAATTTTTTCTCCATTACC CCCATTGACTGAATTGATATCAATATCCCTAACATCATGCATATAGTCAGCTGGCACTGTATTATCAAAATAGG CAGATTTACCATGATCGGATACTGAATTGAACGCCGCCTTGAATTGTGGAGAAGTATTAACTCCACGATCACTG TCTTCACCGTCGACGACACCAGGCTGAGCAGCTTGTTTTGCAGAACGAAGCCATGGAGATGCAACCAAGAAGTT CTGGCGTTTGGAGGGAGCTTTCATGTCGATGCTATACGGTTTCTCAATGCGATCCGGTGGGAGTTCATACAGTT TTTTGCAAAAACGTTCAGAATGTCCCATTATACCACATATAAAGCAGAAAGTGGGTACTCTTTCATACTTAAAA TTGGCATAGAATGTTTCCCCTCTTAATCTTTTAAACTTCATTCGTCTTTTCAAAGGGAGATCAATATTCATCCT AACACGAACCCGTAGAAGCTCTCGCCATACACCTTTGAAGTTATTGGGATCGGATTCAAGAAAAGACCCAATAT ATTTTCCAGCCTGACGAACAACATGTTCTGTTTTGAACCCAGGTTGCAGATCGTGTATCTGGACCCATAAGTCA AGATGGTTAATCGGTTTGGTTCAAGGATTATCACCTGGCTTTAATCTCTCAAGAAGCAACTGTTTGCGATCGTA AGTCCAAGGACTACCAGTGATGACCCTGTCAATATCAATTTCATGGTAGAATTGGAATAAAAATCGGTTTTGCT CAAGTATTTTTACATACATACCTTTCCCTGGTTTCCACAAATCAGCAATGATATTTTGAAACACTTGATAGTCT GAGATTTTCCCAGAAAGGAGTCTCCCAATAAGACATCATCCGTCATCGTACTCAACCTCATCAGATTCAGCTTC TTCTAACTCAGTAACAAACTCTTCTTCTTCTTCTAAATAGATATTGGCTCATTATTTCTCAACATCATTCATAA TTCTACTGCTTGAGGCCATAGTAATAAGACAAACACTCCAATAAGATAAACACAACACTCAGGAACAAAGCTTA C AGAT AAAAAT T C T C AT AAAC AT AC AAAAC TGC C AAT C AC AGAGAGAC AG CAC AC AT GT C AAAAGAC AT GAC T T ATTTCAACCACACCCGTACTCTTCTCTTATCTTTAAATAATATTGAGTTGTGTTTTGTCAAACGATTTGCAAAT AAGCTGACTCATGTTTTAGCCAGGGATTCTTGTTTCTCTTCAGGTCGTGTGCTTCAATTGGAGAATTGTTCCAA CGAAGTGCGTTCTATTGTTATTAACGAGTGCTAGTTAATAAAGTTGCTAAGTTTATTCAAAAAAAAAATTTAAT T T T TAAT T GCAGAGATAT T T T TATAATAAAAAAT T TAAAGAGTAAAAATAAAAAAAAATAAT TAT AT TAG GT AT TTTTGTAATTTTTTTTTTTAATTTTTGGTCAATGTAAATTTTTCTTTAGTAAATACTATAACAAAGGATTAAGT ACAGGTATTACATATTGATATAACAAAAATACAAGTATTACATCTAATTTGCAATTTGTTTTCCTTACGAAAGA AAAAACATGTAGTTTCTTTCTGAACAAAACTACGCGTTTTAGTTGGTGGTCGCTTTCATATAAATATTAATCGA TGTTGAGCTACTTACAATTACATCTCAACAACAAACTAAAAAGAAAAACCATTAGTGGGTGACCCCTTCCACCA CAACCTTTGTCTAAGGTTCTTTATAAATTTCTTATTTTGTTCTTCCTTATGTTCCTTTTTTTGTTTTAATTGTT TACACTGGAAACTTATCTAAGAATATATTTTGCAAAGAATTATATCTTTGGTTTGAAGTTTCTTCCACCATCAT TTTGAACAGTTTCAATTCTATTATAGAGCTATAAAGTTGAGAGTCTTTTATAGTTGCTTAGTGTGCATTATAAT TGTTCGAGTAATTATTTTATAACGATTAATTCTGTAAAATAATTATATGTATAAAAAAAGGTTATTTTATTTTA T GAATAT TAAGT CAACAATAAGT TAATAACAT T T TAAAAATAAAC CTT CATT TAGTAAAAGT CT T GGAAAT GGT CCAAATCCGGTTGTTGGTAGAGTCATAATTCAAACTGAATGGTTCAAACCAATATAAAAGGATTTGGGTAGTTT TTTTCGTTTAAAGGTATGTCATAGAATACATTTTGATATACTTTTGGGTCCATCACTCTAAATGATGATTTTAT TAATTTATCCTTACATTTTTTGGGAAACACGCTTGTCTGACTGAGCATAACTAATAACATAAAAGCCAATTTAT TATCAGTGATCTGTACCACCTGTTTGGAAAAAGTCCTCTGTGTGAGAATATAACAAAGCTTTTTCAGCCCAGCA AATTTACTTTCTGTGATTGTCTTTCTTCTTCTTCTTCTTCTAATTCACAAACAAAGTTCATCAGAAAACCAATA TTTCTGGTCCTTGAAGAAGCTATGACAAAAAGCAAAATTTTAAATCACTTCACTTTGTTGTTGATCCTTATAAT CTACACCTGGTTTCTAAAGGGAGTAACCTCAGAAGATTACATAGTTGGTGATGGTGATGAATGGAACAGCCAAG GCAACTTTTTAACTTGGTCAACAAAGTACAACTTCACTGTTGGTGATGTGCTTGGTATGATCTTTTTGTTTTAA CCCTTTAAATGTTTTGTTCCTTAAAAATTATGTCTGCTATCATCTTCTAAAAACAAGAGTAAAAGCAGAAGTCA CTATTGCTTTGACAGAGAAAGTAAAATCTAACTAAACCAATTGCTCCTGAAAAAAACAACTTTGCTTGGATTGG AAATCAATTTTTTTTTTTTATTTTTGTTTCACCTAAGATGTTACATGTTATGTAGTTTTCAAGTACGTAAAGGG ACAACACAATGCATATGAGGTAGATGAGAAAACGTTTCGATCATGTGATGCGAGCAGCGGCGTGTTGACAAAGT ATGAGAGTGGAAATGATAAAGTCAAACTCACTGAAGCCAAGAAGTATTGGTTTATATGTAATGTATCAGGCCAT TGCTTTGGTGGAATGAGATTTAGCATTGATGTTAAACAAGGTAATAATCCCTCTTCTGATGATACCAATCCAAC CACAGATGGTTCTCCAACTTCTCAACCTGATGAGCTGCCAGCAGTTCCACCTTCCAGCTCTAGTAGAAGCACTT TGTCCAGTGACACTTGGAAAAATGGGATTTACACTTTGGTATTTGTGATCTTATTCAAATTGTTTTATTAATGA AATCAGCAAGTTTTTTTTTTTCTTGTAATTGTTGTGCATATATAGTAACATTCTTTCAGTTCATAAAACAACCA CTGATCTTTAGCTTCCCAAGATTTGTAGACAGTATGGTGTTGGGGAAGTTAGTGTATGATGGCTGAAAGCTATG TATTTGGTACATTTATACATACACAACGCACATAGAACTTTATTGGTATGTCTGAAATAAATTTTTGTGTAATG ATCAAGATTATTAGCAAAGAGCACAAGTGGCACACTAAGCATTTAAACATATAGTGTGCTTTTTCTATTTCTGT GGACCAAACAAAAACAGATTCTCAGCATCAAATCTGATACACTTTGCTGAGTTACACTAGAGTACTAAATATAT ATGACATATTGAAAATTAATAGATTATTCAGTAGAAATCTTGCTTCCATTTAAAACATTATGTTTTTAAGCTTG AAGTGAATTGAGTTCTTATCTCATTTGAAGAACTGTAACTTAATTATGAAATCAATAAGAGGGCTGGTTGTGAA GATGGTGCTGATAGTCAATGCTGCTGCTATTATCTGAAACTTTAGACATCTCAGACAAACCTCTAGAAGGATTC CTTGTCAGCTCCAGCAAGAAGTCTCTCAAGTGATCAGCTTGGACTACTATTTTACTGTCCTTCTTCTGAATCCA AAAAT T T TAAG CAACAAATAGTAGT T TATATAT GTAGAT GT GAAAGAT C T T G C TAAGAAAAAAAAAT T GAT TAG AAGCATTCTACCTCTACTTTAAGAACATTGGAAACAAGGTTTCGAAATGTAGTAACGTTGGCATTAGTTACTTC AAGAGCTAGAGAATTCAAAGCCTCCATCAATCTCACAAATCTTCCAGGCTTGTGCTCACAGAATACCTTCACAA AGAACTCATTTCCATCTATTTGAGCCACTTCCACTTGCGGCTAAATTTGTTTCAAACATGTTATTATGAAGCTT AAAGCACAAATGTATCAGAACGTTAAGAAGAGTAGAAGAACTTATGTTACCTCCATCTGTGGTGCCTTGTCATT AGTTGTATCTGAGTCTTGGTTCTGCTTTGAGATGCTTCCACTCCTTGATGTTTCCAAATGAAATTCATTTTGAA CTTTTTCATGCTCAGGCCTAGACCTGAAATTAGTGTCGTCGCGACTCAAAGTCTCAGACAGAACATTGTTTCTT TTACCATTAATGTTGGAGTTTAGGCCATCATTGTCTGATTGCTCCTCTAGCTCATCTTGAAGCTCTTTTGCCTG ACTCTGTAAGTCTTTTACATACTCAATTGCATCCCCAAGTATGGAGGCCCTGTCCAACTGTGGATCAAAAATGA GATGAAGACCTTAGATTTTACAACTATTAGTTCCAATTTAAAAAAAAAAAAAAAAGCATTAAATATCTGTTACC TTAGAAATTTTGGGAACCAAAGACCGAAGAGTATAGAGCCTTTCATTAAGCTTCTTCCTTCTTCTTCTCTCAGC AAAAAGGTTCTTGCACTGATCTGATTTTCCTGATTTTCTCCTATACTTTCCATTATCCTCATCATCAATCTGAT CACTACAATCCGAGACTGAATTTGATCTTCCTTCTTCCTGTTTGTTCGAGTCCTTTTCATTACCATGCTGCTCC TTGTTTTCTGTTACTTGTTCCATAAATTGCATATGTGAATTGGTTGTGTCATTCATCATGGATGGCTGCATGGC ATCCATTGAAGAATCCAAATGCTTATCTGGATGTACCGATGGATCATGAGAACATTCAGGAAAGGCAGCATCGT TCCTGGTCCTACTTTCAGAATTGTAACCAAATTGCTGCAAGCTCATTGGGGAATTGCATAGACGAGTTCGATCA ACAGATACGTCATAAGACATGTTCAGGTTCTCAAATGCTGTGGAATTAGGTGCCGGCAGATTATTGTTGGAATC CATTTTGTCAGTTTCATTGCCCAAAAATGGCCTTGATTGGCTTTCATTCATTACATTAATGTTGACACAAAAGC TTGTGTCCATATTGTTTGTGCTAATCAGGGCTTCCTGCTCCATCAAAATGTTGTATTGAGTTGTGATGAAGTCT ACCACATTCTGATCTTCTGATATCTGTCAAAAGAAACTCTATTATAATACTTACTTGGAGTGAAGACAAGAGAA AATGATTATTTGAAAAGACAATACTCATACCTGTTTATTCACAAAAAGCTCTACCAATGCAACTGGCAATGGAA TCAAAACCCTGGTCCCAATAGATTCCTGCAAGTTAATATAGCTTTTAGACAATGGTTGAAAATTAGAGAAGGGT TTAGTTTCAAAAACAACTTACCTCGAGAGCATGTGAGCATGTGACAGTTGAAAAGTTTAACCAGCTGGGTTGAT TGGAGACCAAGGTTTGTGCATAAATTCTGTTCATAATCTTGTGTTAAGTATAGGTTTAGGATTCATAACATAAA TACCAGAAAAAAAAAAAATCATTTATAGTAAAAAGCTAAACAAAAGAAATGGTTAGTACCCTGAATCTGAAGGC ATTGAAGTTGGCAATTGGGCAAGAAGTTCACAGGAATTAACTCTTGGGTGTTGACATATGGTATCCCTGCATGG AAGGACTGGAGAAACTGGAAAAATCAGCTCTTCTTCTCCACCATTTTGTGTGCTTTCAGTCCCAGCACAACAGC AT C T CAT C CAC T CAATAAAC C TATAACAAATC C CAAAAAGTAACAT GAAAATAGACACACAGACACGTATCAT T TAATTTAGTTAGTCATGGACAATTTCTCTAACCTTTGATCTTCACTTAGTTTCCAGAGAACACAATAATCCCAT CCTTTCGAGCCCACTAGAGCTCTAACCCTCTCCATCAGATACTGCATTATCAGGTCCATATTACCTGCAAAATA ATTGAGAAAAATGCAAACACTTCAAATTCTAAAGAATCCCTTTTTCTTTTTCTTCTTCTTTTCTTTATTACTAA AATGCTAATCTTATTATGACACTCACAAGTACCAAGAGAGATAGCAATGCCTTCTGTTGTTCTCAAGATAAATA TGCTTAACACTTTTGACTGAATTTAAGTATTCATATGAAAAAACATAAGCAGGGTGGAAGAAGCAAAACTACAA GGCAGCAGAAAATACAAAGGAAAATCTCCATACAAAGTTCAGGTGACAGGAACTGATATAGTTGCACCAAGTTT AAGCTAAGACAGCTCCCCCCACCTCATACCAAAGTACCTATCTCATCCAATAAGAAAGAAACTAAAATAACAAT AACAATAAAAATAAAACAACTTTAGACCCTTTTATAAGTTTTTTTTTTTTTTTGAGGTTGGGAGCTACTTATAT CGGTAGGTGAACTGCAGCCTAAGCATGAAAGAAGGAGAAATTTTCATATACAATAACTAATATTATAATTATAT CTAACTTTTCTTGTTATGGAAGATTATATAAGCAAATATTTTAGGAGGAAAGAATTCATTATTAAAAAGTATAT AAATATATATTAAGTTAGTTATAATGAATGAGAGCATATTGCATGACCTAAGAGTGTGGCGTTTGTAACCTTAA CTACCTTGAAGTTTTTCGCTCGCTTGGGATTTCTATATTCTTACATGTTTCAACTACTCTATAACTTTGGACTA GAAATGGAAAAGTACTATACGTAAACAAATTTGTAAAAGAAAATTCAGTTGTTCCCAATATAGATGTAAAAGGA AAAATGACAAGTCTTCTCCAACTTGTTGGAAAAGCCTGAAAAAGTATAAACTCGATCTTATGTCGTTTCCTTAG AAGTATGATGATGCTTTTGATTGATTGTACATGAATGAGTAGTAGTCTTAAGATCGAGTAGTATTTGTTTCCCT AAAGGAATAATAATAATATATTTATCGATTGATTTTAAAAAGATAGAGGAAAGAAATTAACTAATTACACAAGG AAATGAGTTGGTAATGATGAGAGAAGGCTTATTGCATGACCCAACTTAAGGCCTACAAAACACTAACTACCATG CTCAAGTTTATATTAAATTATATTCATTTCGTTTTGGTTTAGTATATTATTGCTTCATGTTTCAACTACTCTAT CACTTATTGTATCACATTTGGGAAACATACATCACACCCTTCATTTAGATAGGGAAATATATTCTGGCCTCATT GTAGT GAT TT T GT T GT CAC C CAGC CAT TTAGT T GAAAT T T TAT TTAAT CAATAAGAAAAAGATAAGTTAT C CAT TTTTCTCTATGTCCTACACTTTGATTGAATAGTGATGTAAGTCAGTATAAAAAGATGATTTTCTTTTCATGCTT TGGAGATATTAACGTTCGACAAGCCAACATTAAAGCTAGACAACTTTAATTTTCAAAATTTGGAAGAAATGCCG ATAT C T T T T TAGACAAC CAATAAAATAGATAAATACATATAATCT C GAAAAAT T GACT CAAAAT GAC C GAC GAA GGCAATACTTTTTGCCGTTAATGTATGATTTATAATGGGAAATTTGATTATTTATACATACTAATAGGAGAAAT TAATTCTCTAAACTTATATATTTCAAAATTTTAAAAATAAGTCAAATTTACATTTAAACTAAAAATACCCCTCT CATTTTCAAACCACACTTCTCTCTCTAACCTCTGTTCTCTCCCCTCTCTCGCTCGATATCCCTTCAACATCCCA ACTGAACCCACGCGAACCACCCCACAGACCCACAAAATCGGACCCATCGAACCCCTTCAACATCCCCACCAGAC CAGTGGCTCCCCCCAGACCCACGGTAAGACTACCACTAGACCCACGATACCCGAGCACCCCAAGAATTAAACAC AATGTCAAAGGTGAGTTCGTGTTCCGTTTTTTAGTTTTCAGAATTTCAGATCTGTGATTTGGACAAGGTTCGAT GGGGCCCGATAGGGTCGAATGAAACTGTGAGTTTGTGAGGTAGGAGACGTATGGGTCTGATTGTGTCCGATGCG GGGTCCGATGAAGGGCATCCAATTGTGTAAATGGGGGTGATGTAAATGGGGGGTATTTTAGGAATATCATAAAA ATTGTCATATCCTACAAATATTAGTTATTATGAATTTAGGGGAAATTTTTTAACAAAATGTAAGCATAAAAATT AAATTTCCCTTTATAATTTTATATGTTATGTCGACAATGATTGTTGAGGCAGTTTATGACCAGTCACCTGTGAT AT GT GTTAACGCAGATTTTT GTTAACCAAATTTGAGCCTCACGAT GATAATT CAACACAGAAAAAAAAAAATAA AAGCTATAGAAAAATAATATATAAACACTGGGTTTTTTTACGTAGTTTTGCAGTTAAAATTATGCATAGTTCAT GAGTCAATCTTATTCAGATTTTTGAGTATTTTTTTTTCCAGAGCCTCTTTCATAAGAGTTTTTTCGTCCTTTTT TTGAGATTGTTTGCTACTATTATAATTGCATAGTGGCAGTTAATTACAAAGCTGACCAAAATATGATTACAGTC ATTATCCCTAAAATCGTGGGATCTAATTACATAAAAATGTAAATGCGGTGTATGATTTTTAAAATCATATAGCT GTGATTTTTCTGCTTTTATTGGGTCAAGAATATCGTGTATTAAACACATATTTAATTGTTGAATTCGGAGATGT CTCGATAGAAACTTGATTGTAGTGATCTCAATGGCGAGCTGAAACCATTCTTCTTTCTGAGGTCGATAAGATAT TTCTTATGTCGATCGCGGGTATACAGAAATCTTCTGACTTGCCAGGATTATGAGCCTCGCTCCTATTAGTGGGA TGAAACTATAGGAAGTTTCTTCGTAGCGAGATGGCCATCTCACATTCTTGTTCTTCGAGCCGATTCGACTTTTA CACTTGGTTTGTTTATTGCAAGCTAAGGTATATGTTACCTTTCTCTGGTGCTTCGCTATTCTCCTTCAGCAACA TATGATTTCTCCTTAATACACTAAGTGTCAGTTCGTACATTAATTTCTCGCCTAAGACCTTATAGTCAGCAGGT TACAAATATACTCTGATACTTCCGCAATTAATGAGTTATTCCATAAAAAGATATTGCACCGATTCACATTCCCT TTACCTTGACTATTATCCTCCAGCCAAATTTCGGGTATAACAATTGCCCCTCAAAGTGCTTTATAACAATTACG AAGGGCATTTTTGCCAAAATCCTTTCTTGGAAAGGACGCCTCCTTTGATTCTGGTGGTTGTACGATTTCAAAGA TTATTATGAATCACCTATTCATATTCTGCACGATTCCCAGCTGTTAGATTTCTCAATCCTTGGCTTTTTTATCA ATGAACTTCGTGTATAAAAGGGGGGCTCAAGCTTGTCATTTTTCACAAACCTTCTAGCTCTTTAGTGAAATCCA TTTCCAGAATGGATTACTTCCTTCCTGCCAAAATGTCTCTTCTGAGTTTAGAAGTTTATCCTTCCAGACTCATT ATATCCAGATGCCCCAACCATTTTCTATTTGTTCCTACAGACTTCAAGCCTCAACCGGAGCTGGAAACGATGGA GTCCAGGGCTGATAAGCTTCCGACCAATCCAAACTAGGGGAACCCCATAGCCTGCCATGGCCGATCCTCACAAG TGATCCTAGAGGGTACAGACCTTGTGAGCGATCTCAAAAATCCTCCTCAGGTTGATGATGAATTCTAGAGGAAG ATTGGCATGGTTTCAGGTGGATCTGGGAAGGAAGTTGGCGTGGTTTCTATCAGAGAATCCAGCCAACCCAAGAC CCTAACCTGTGCCAGGAGATCTTTGCATTAGCTTCTCTCCAGTGTTGAGAAATTTCCTGAGGCTGGGAAATAGA TCTCGGCTTCAGGAAATGGGATCTGATGATGAGGAGAATTGGGTCTGTGCAGAGATTAAGGCTCTCTACGTGCA GACGGTCTTTTTTGTCTATGTGTAGCCGGTCTTTCTCCAAGCTATATGTAGACAGTATTTCTCTCTCATCCTCT GCTTAGGAGAATGACTTAATGGTTTGCCCCTAGTGGCATTTTTGTAAAGACAAGTTTGTAACCAGTCTTAGGTT TTTCTTATCTTAAATGTCTTGTACTGTTTTCATTAGAAATTAATACAATTGTATTTGCTTGCGTTTTCATTCAC AATGTAAACAACTATATTTTGATCAAATATACATCACTTTTGGGTTTCTCCATTGCTCGTAATCTTCTTATAGT TAGTTATCTAACAAACTTTATGATTTTTGAGCGGTTGTCAATTCTGCCTCGCAATTGCAGTTATAATCTGCCTT AAGCAAGCTTTAAATATACTTTAAGGATTTTATCACTTTGTATATTTTTTATTTGCTCGTATGAATAGTTATAT TATCTATCCCTTTTCTAGGTTTAAGTACTTTTATCAATGCGCCGTGATGCCTCGTTTAGTACTTTCCTAACTCG CGAGAACTGTTAGTATTCAGTTTTATACGCGAGTTATAGCTTTTGATTTTCCTCTCCCGTCAGATAGGTTATAA ATCAAAAATTATAGTTGTTTGATCACTTCTTCGTCAAAAAGGTTTGATCAAGGTGTTATAACGATTCGACCCAT TTCTCGTCAAAAGGGTTTGGTCAGGGAGTTATAACGGTTCTACTCCACTTTCGTCAAAGAGGTTTAGTCGAGAA CCTTTGAATTTTTTTTTTAAAAAAAATTGAAATGGCGAGTTTGCATTATACAATGTATTTCCAGATGAATTCCG GTTAGTCATACATAAATGCCCCTTAAGTAATTTAAAGAATGAAACTTTGTAATTACTTAATATAAATGATTCTC TTTATTCATAACTGATAGAAGGATCGCAAATGTTCTGCGTTCCAGTAGTTCTGTAATAGCGAGCCATCAAGTCG AGATAAT TTATAGACTCCGACTC CAAC Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 7644547 7649546 1 5000 upstream regulatory region gene 7649547 7652154 5001 7608 genebiotype = protein coding 5kbs downstream 7652155 7657154 7609 12608 downstream regulatory region mRNA 7649547 7652154 5001 7608 gene=LOCl 15706375 exon 7652054 7652154 7508 7608 product=transcription factor ABORTED MICROSPORES exon 7651785 7651967 7239 7421 product=transcription factor ABORTED MICROSPORES exon 7651599 7651677 7053 7131 product=transcription factor ABORTED MICROSPORES exon 7651460 7651528 6914 6982 product=transcription factor ABORTED MICROSPORES exon 7650689 7651378 6143 6832 product=transcription factor ABORTED MICROSPORES exon 7650296 7650598 5750 6052 product=transcription factor ABORTED MICROSPORES exon 7650035 7650211 5489 5665 product=transcription factor ABORTED MICROSPORES exon 7649547 7649940 5001 5394 product=transcription factor ABORTED CDS 7652054 7652154 7508 7608 CDS 7651785 7651967 7239 7421 gene=LOCl 15706375 CDS 7651599 7651677 7053 7131 gene=LOCl 15706375 CDS 7651460 7651528 6914 6982 gene=LOCl 15706375 CDS 7650689 7651378 6143 6832 gcnc=LOCl 15706375 CDS 7650296 7650598 5750 6052 gene=LOCl 15706375 CDS 7650035 7650211 5489 5665 gene=LOCl 15706375 CDS 7649779 7649940 5233 5394 gene=LOCl 15706375 >AMS amino acid sequence from CBDRx (SEQ ID NO:9) MDLIMQYLMERVRALVGSKGWDYCVLWKLSEDQRFIEWMRCCCAGTENTQNGGEEELIFPVSPVLPCRDTICQH PRVNSCELLAQLPTSMPSDSGIYAQTLVSNQPSWLNFSTVTCSHALEESIGTRVLIPLPVALVELFVNKQISED QNWDFITTQYNILMEQEALINTNNMDTSFCVNINVMNESQSRPFLGNETDKMDSNNNLPAPNSTAFENLNMSY DVSVDRTRLCNSPMSLQQFGYNSESRTRNDAAFPECSHDPSVHPDKHLDSSMDAMQPSMMNDTTNTHVQFLEQV TENKEQHGNEKDSNKQEEGRSNSVSDCSDQIDDEDNGKYRRKSGKSDQCKNLFAERRRRKKLNERLYTLRSLVP KISKLDRASILGDAIEYVKDLQSQAKELQDELEEQSDNDGLNSNINGKRNNVLSETLSRDDTNFRSRPEHEKVQ NEFHLETSRSGSISKQNQDSDTTNDKAPQMEPQVEVAQIDGNEFFVKVFCEHKPGRFVRLMEALNSLALEVTNA NVTTFRNLVSNVLKVEKKDSKIWQADHLRDFLLELTRNPSRGLSEMSKVSDNSSSIDYQHHLHNQPSY See APPENDIX A for the eight most common genotype sequences in Cannabis accessions for AMS. Gene name: bHLH91 CBDRx assembly gene ID: LOC11571337 Number of Isoforms: GCF_900626175.1 / GCF_900626175.2 5 and GCF_029168945.1 6 Number of Indels: 3 Number of Exon SNPs: 16 Description of variation: this gene is relatively well conserved and has 16 SNPs. There are a few insertions in the upstream and downstream regions outside of the coding region. There is considerable variation in the start position of the second exon, ranging from a dozen base pairs upstream to spanning the entire length of intron one, merging them into one large exon. > bHLH91 nucleic acid sequence from CBDRx (SEQ ID NO:2) GTTTCTTTCT TAAATAAAT T GTACTAAATATAT GT TAGTAAAGAAGATAT T GAAT GGAATATATAAT TAT TATA CAAAGCATATATATATATGGGTGCACTTTTAATGGGTATTACTATGAATGGGTAATATTAGAAAATTTTGAGTT TTAATGATTAAATTTTCCATTTAATTAGAAAAATTTCTCATTTTTACATTATATGGGCCGAGATTGGTCCATCA GCTGAAAAAAAAATAATAAAAAACAGTTTTTTGGCAAAAAAGTGATAAAAAAAAGTTGAATTTGACTTAAATAT T T T AC AT AT AAAT AT AT TTTTTATATAGT GT AAAAAGAG GT AT T T T T GAT TTTGTTTT T AAT T AAGT AG C T AAA TTAAGCATAGAAATTCAAATTTCTCTTTTATTATTCGCTATCCAACTAAAATCTAATGGTTTAATATTTTTAAA AT TAATAT TAATAGTTAAATT T GT T TATTAC C CT T TAATAAGTAATAC C CAT TAAGAAGTAT TT CATATATATA T G GTAGAGATATAT TAGAT TAAGAAAGAGTAACTATAAAGAT GGTAAGTAGTAT TAAT TAGT TAGTAGAAAGAA AGGGGTCAAGAAGAGAGGAAGAAAGATTGGCAACTTATTAAAAAAATTAAACAAAGGGACTATTAAAATGAAAA GAGGTTATACCCTAAATATGAAGTTTAATATAGTTATACAAAGCACTTCTTAAAAAAATATTATACTTAACATT AAATTTGAAAGTATGAATGTTGGTAATTCATCCAATATTCTTGTTTTCTTCCTTGAATATTTATAAATGGGTGT TTATTGGACCACATCCAATATTTTAAGGCTTATCCAGATTCGATCTATTTATATATTAGATGTTAGATTTACCA TCCGATCTGATCCAATTAGTTTCAGTCATCCAATCGATCAAACGTGCATTGGATGTTGGATTGGATCGGTTTTA TCCATTGGATGTATTTCATATATATTTACTGATTTAATTTGAGTTTACTCAATATTTTAGACCTAGTATTTTTT TAGATCGGATCGAATCTATTTAATATTTAAGTCTAGTATGTACTGAATTAGACTAGATCCATCCAATCTAAGAA AAAAGAGTAAAAT T T TAAT GT TAAT T T T CATATATACATAGAGAT T T TAC GTATAACAATATAAAATCTAAT CA CTCATTCAAACTAAATGAAAATACTAATTAATTTGATTGGATGTTAGATGTATTAGATTTCTGGACACCCCATT CACCATCTGATCCGATCCAATTGGATATTTAAAAAATAACATCTAATCCGATCTGATCACAATTGGATATTCAA TGGTGGTCGGTTGTAATTGGATCGGTCAGTTATCATTAGATTTGATCAATGTATGCACACCCCTGATAGTGTAT CACACATACACAT GTAGT TAAAGT T T T T GAATAGAAT T T TAGGAAT GCAAT TACAAATATATATATAT GTAT GA CTATATAAAGATTTTTTATTTAAAAAAAAATAATAAATAAAGATTTTGAATTTATTTTTTTTTAATTGATTGAT TAAGTAATAT T TATAAG C GAAAT TAAAAAAT TAAAAAAAAAAT TAAAAT GAGATAT T TAAAATAAT T T TAAAT T ATTATGTCACCTCATACATTATAGGGATTTTTTCAAAAATATAAGTAAAATAGAAAATAATTACAAAAATAACT T AAT AAAT T T C AAT T AC AAT AAT AT C T T AC C AC AT CAT T AAAAAAT AC C T T AAT T T C AAAAT TAT AT AC C T GAA TGAAACTAATCCCGAAATTCTTTTTTTTTTTTTTTTTAATTTTCCTGCGTTTCAAGAGTAACCTTTTGGTTACT TAT GAT T T T GATAATAAAC CAATAAGTAAC T T T T TTATAAAAAAGTAT CACT T T TATAT TATATAAT T T GAGAT ACATTTTGGTTACCTCTAAAACTTATATTTATACACTTTAATAATCAATTAATTATTTAGGTTATATTAATACA TTTGAGTAATTTTGAAGTTTATTTTCAAAAATAATGAGTTGTCATCCAGTAAGCTACTATTTTAGTAAAATATA T T T T TAAAAAATATATATCATAAATGTTTAAGTCACCTATATGTTT TAAAT GTATAT TAATATATACT T T T T GA TTAGTTAAATAAAGAATGAAGTTGTAAACAATTTCTAAATTATTACTAACAAGACATTTTTTTTTGGACCAATT AGTTATGATTTCGATAAGTTACCAATTGGTTACTTTTGATAAAATCATCAATTTTAAAATAAAAAGATGAGGAT CAGACCAAATATCCTATAGTCAGATATTCCTATACTCATATATGCCAAACAAACTTATTAGTTTATTTTGGCAT ATTGAAACAATTTTTCATTTTTTGTTTACATTTTTCGTATAGCCTACCATCGTATATGCTAATAAAACTATTGG TTTATTTTGACATATTGAAACAAATTTACATTTTTCTTTTCCTATATTGGAACAACAATTCATTTCGTTTTATA TAGCTTACCATTATATATGTCAATAAACCTACTAGTTTCCGTTTGTTGGAACAATATTTTATAGTTTTCAATAC AAAGTAAAC GAT AAT CAAACAT AAT GATATACAC CAATAAAC CTATTAGTTTATTTTTCGTATATTG GAATAAA AGTTCATTTTTTATTTCAAACACAATGTGTGCTATAAAATAACAAACTAATTAGTCTATTTTTTTCAAGTCTAG ACATCAAATCTGGATGTATATAAAGAACATGACTGTAACATGCCTTACATAGTAAACCTAGCTAACAATATATG CACTTGTCAAAGATTTGAATATGATAAAATATCTTGCTCACATGAAATGTCTTGTTCAGAAGTTTATTTGGCAT ATATGGCTATACTATGCCTATACTCATACATGCAAAATAAAATGATTTATTTTGACATATTGAAACAAATTTTT ATTCTTTGTTTACATAGTAGCCTTTTCGTATAGCTTATACTATCGTATATGCTAATAAATCCACTAGTTTATTT TAGCATATTGAAACAAATTTTTATTTTACTTTTCGTATATTGGAACAACAATTCTTTATTTCATATAGCCTACC ATTATATATGTCGCTAAACCTACTAGTTTTTATTTGTTGGAAAAATATTTTTTTTTCAATACAGAGTAAACTAT AATCAAATATAATGATATACGCTAATAAACCTATAAGTATACTTCAAATAATAAAACAAATAAGCATATATATA AATTAATTTTATTTAGTATACTTCATAGTAATATTCAAATAAAACGATGTAATATATATTTCGTCACTGATATA T TATATAAGTATATAT T C T GAAT T T TAAAAATAAGAT TTATTTGTTAAC TAAT AAAAAT T GTAGAAT GATAAC T AATGAGTATATAATTTGAGATATATTTTTTTTTCTACCAAACTTATTTGTATACATCTTAATAATGAGTTAGTT GT T T T GTAGGT TATAT TATAGTAT T T TAT TAT T T CATATACAATT GAAT CAAAAT TAAT GAGTAGCGATATAGT T G GT TAT GAT GT CAATAAAATAT TAAT TGGTTACCTTT TATAAAACAATATAC T T TAAAATAAAAATAT TATAG AAAAATTCTACAATGCACACCCTTAAAATGGATATATTGATGCACCCCTATCTGTAGAATCACCCAATATTATA TATGTAACTTTCAATATTTGGAGTAGTTATTTTTAAAACTATATGTTCATTTTTAAATTATATTATAATTTTTT AAGATAAT TTTATATTATTTTCGT GAAAAATATACAT TTATTTTATTTTT TAAAATAAAATAAAAT GAGT CACA TAGTTTATTAACAATTATTTGATTATAAACTATTACACACACAAAAATAACCAACTACAATTTTGAAAATAACT AAGAAAATGGTGGATCATGATTTTTTTGCAGGTTGTCAAACGTGGAGGGAAAGCTATGATGGGCTGACGTCATC AAAAGGAAAACTAATTATTTTTAAAAGGGTATGATAAAACGGCATAATTTTGATTTATATGAGACCCACCATAT T T T TAGAAATAAAT TATAAT T TAC GTACT T GGGT GTAAAT TAC CCTACAT TATAT T TATATAAAT GAATATAGA TTTATAAATTTTGTAAATGTACATGTAACTTATTATATAGATATAATATAAATAACTCACTCTTTAGTGTGATT TATACTTCTACATGAAATTTGGATGAAGCATATTCCCATGAGTCGTGAATGTTATTTTAATAAATTTCAAATAA ACAT TAAACATAAAT GAAAAGT T T CAAAAAATAATAATAAT TATAAAAAAAAAGT GT GATT T TT GT CAGTACT T TGAAATTAGAGGATTCCTTTTGGTTCTTATCCAAAAATATAGTACTAAAGAGGGTTTTGACATGATAATATTGG CCTGGCCAAGTAGTTAGCTTTTTTTTTTCTTTAGTGAGCAAGTTTTATAGATAAGAAAGAATTATGAATTTGTT CGAGTGTAAATGATCCAAAAAAGCCTTGCTAACCAAAAAGAAGGGTAAAGACAAAGAGAGACTGATAAAAAGAA GCCTTTTTATGAGGGAAACAAATTAAAACACCAGTAAAAGTGGCAGAAGCAAAGATTGGTCACTCAAAGAAACT ATATTAGGAAACAAAGCAAAAATCAATCAAGAGAGTTTCATAAAGGAATGAGATTTTCATTCTGTAAATGCTAT C CAAC CAAAT C T CAAC C GAT T T T CAT T T GAAAAATAC TAT G GT TAGACACAAAGAGAT CCTAACCGTC CAT TAC AGACAATCATGTTTGGTTATATAGCAAAACTCTCTTTATATTATTTTTAATATTCTTGATTAATAAATAAATTA AAGAAGGAAATACATTAGAAAAGTATATAACTCATTCTTTTGTGACTTTCTAACTAACTTCAGTTTGGGTGTAC TtTTTTGTTTTTCATCTTTTTTTACCAAATTGTAAAGTATATAACTCATTTTTATATGTACAAACCTACCTAAT CACCTCCTGCCTCCCCCAGTAAGGCCATCCATCCAATCCCTGCTTTAGCAGTATACTCTTTTATTTCCTCTACA ACAGAAAGTAGAAACCTTTTACTTGAGGTTGGACTAGTTTGCCTATATATTTTTCTTTATTTTTTTCATTAAAA CATTACAATAAATTAAGTAAACTTGGCCAAATTATGTAATCAGATGGAGAAGTTTGATGTAGGTTTCTGTTCAT TTCTTCCATGAATTTCCTGTTTTTCTGAGTTTTATTTGAAAACAGATGTCAAGTAAAGATTTCCTTTGTCTGTT AAAAAGGTTATGAATGAATAAAAATGATCATTGTTGAGTCAAATATACATCTACATAAGTGCTCATTCAGTCGT TCGTTTTTGTCTAACTTTGTTTTATTTTTGATATGTGTGATCATCTTAGCAGACTAGACTAGACTATACAACAC TACAAGAGAAAGGTGGGGTTTTGTGAGTCATTGAAAGTCTTACCAGAAAATACAAACAAACATGTATATGGATA ATGGAAGCTGCAGCTTTGATCCCATTGACACAGCAGAAGCCACTGCCGTTGGTCCTGAAGATGCCTTCAACTCC CAGCTGCCTCCTCCACCGCCACCACTACCATCCATGACGGGCTTTTCAGCTGCTGATGAGATTACTTCAGCTGC AGAAGAGCTTGCCTCAGCTGTAGAAGAGTTTGCTTACCACCAACATCAACACCAGCACCAGCACCAGCACCATC ATCTTCTTCCTAATTCTCAAGAAGAAGCAGCCATTAATATGGAGCTTCAGCAACAACTCGCATTCAACATGGAC AACTCTTACAACACTGATATCAACACCACTGCTCAGTTGGTGTCATTTGATCATCTTCAGCATCACAATCATCA TCATCATCAGCAGCAGCCTCAATGGGATAATGAAGACATGTCTTACAACAACAATAATCATCGCCATAATCAAC ATCATCAGGAGCCTGCTGATCTCCTCAGCCTCTTCCACTTGCCCAGATGCTCGGCCACCTCCTCCTCATCCATC ACCTTCACCAACCCGACCCCACCGAAAACAGCCAACTTCCCAAACACTTCCCTGGCCTTTCTGGGAGATGTTCC AATGGGAACTGACAGCCCTCCTTCCTCGTCTATTCTGTACGACCCGCTGCTTCATCTGAACTTACCTCCGCAGC CTCCTCTGTTTAAGGACTTGTTTCAGCCTCTCCCAAATGGCTATGCCTTTCCCTCCTCATCCTCAAGAAATGGA TCCTTGTTCAGTAATGGTGTAATGGATATGGATGGAGAGGGGAGTGGTGGGGTTTATCAGGATGGACTTGAAGG AATGCAGTTTGAAAGTGGTGTGTTGGAGTTTGCAAGGGACAGATCTTATATTGACAGAGGAAGAGATGGCAGAG GAACCAAACACTTTGCTTCAGAGCGCCATAGGAGAGTGCAGATGAGTGACAAGTACCACGTGTTAAAGAGCTTA GTTCCTAACCCAACTAAGGTAATTTGTATAAACAATAATAACCTTTCTTTCTCTGATGTATTCAACTGTTTAAC CTCTACATACATACATTTAATTTGTTACAGATTTAACTGTATCTCTGTGTGTGTGTGTGTGGTATGGTTAAAAG AAT GATAGAGCAT CAGTAGT GGGAGAT GCGATT GAGTACAT CAGAGAGCT GAGGAGGACAGT GAATGAGCTAAA GATACTGGTGGAGAAGAAGAGGAGAGGCATAGAGGCTAACAAGAGGCGTAAATCAGAGGAAGATACTGCTGGAG AGGTAGAGAATTGCAACATCAAGCCTGAACCTGATCACTCCTACATAAGGAGCTCTTGGCTGCAGAGGAAATCT AAGGACACTGAAGTTGATGTTCGCATAATCGACGATGAGGTAACCATCAAACTCGTCCAGAGGAAGAAGATCAA CCTCTTGCTCACTGCCTCAAGAGTTCTTGATGAGCTTCAGCTTGATCTTCGCCATGTCGCTGGTGGACATGTGG GAGACACTTACAGCTTCTTGTTCAACACCAAGGTTAGTTGGTTCTACTCTACTGACCTATTTTTCTTATTATCT ATCTTTACAATTTGACTTTGTCTTCTAGAGTTGGTTGGTTGGTGATGATGATGATCTTGGTGTGTGTTGCAGAT ATATGAAGGCTCTTCTTTGTATGCAAGTGCCATTGCCAACAAACTCATGGATGTTCTGGATAGGCAATTTGGAG TCATTCTTCCACTTCCACCTACAAATAGCTATTAGCTATTTGACAATCTAAGATGTTATCACTTTATAATATAT ATGTTACAGTTTAATATGAAGTTGGTAGACTGAGAGACTTATTAATAGATATAATAAACCTTCGGTTGTAGGAA TGATGGTTTTAGACCTCATTCTGACAATTGGGGGAACATACATATTATAAGTAGAGGTTATGGTTATTAGTATA CTTGTGTTGTGATCTCTTAATGCTACTAACACTTGAGGAACACTTGAAGTTTGGCTTATTTTCTATGAGAATAA TTTTCATCTCTATGCTAAGGCTTCCAAAGAGAGCTGGCCTAGACTTGAAGCTCAATCTATATCTTTCTTTTCTT TTCTTTTATGAACAAAGGATGCATGCATGAACAAAATTTCCATGACACCTTACTACATGAAAAGGTTTTGATCA TTTTGTTCTAGGAGAAATTGCTTCTCATTGTGATGTATTGGTZ\AATGGTAATGGTAACCTTGGGCATATTGGCT GGTCTAGTTCATCTTAGTGTTCACCCGTCTCAACAACTACAAAAGACTACTTGTGGGAAATATTGAAACCGTTA TTTTCAATGGCATCTCTTGTTAAATACAACACTCAAGAATGCCTTCTATTACAAGGGGATATCAGTTTTATAAT TAAGTTAAAGAATGATAAAATGCAACCACATTAGTGTAAAGTATCAAATTTGTGATGCTTGCGATTTTAGCCCA TTAACCACGTACCCTTTACACACCCTCCTTTTCTTGAGCATATATGAAATATGTCGCTTCATCTACTTATCAAG CAGTCCAGAATATGAAGAATAAAATTATTTCTTTGTGATCTTGGGTGATTTCATGGAAAATCAACAAGAACAGC AATGTAGCAATGGTTTAGGAATGTGAAATGCCTCAACGTGTACTAGAAATTTCCTTAGTGAATCATCATAGACT CATAGT GCTAAAAGGAGCAGTAAAGGAACACATTACAT GGAGGCT GCTAAT GCCCGAAAAGCACAAAT CACAT G ACAACTGCTTTCATGATGTTAAAAACTCTAACTTATTGCCTCTTGCTGTTGAAGACTCCAATTGGTACTTCATG CAGCTCATGACTTTGACTAAAATCCAACACCACCTATGCTGCAGTTCAGATTGTTGTTTGTTGAGGTGTCCTGT AAAAAAAAAATCTGCATAAGAAGATCAACCGGTTGTCTCTGTGTCCAAGAACTTTGGTTGGATATATAAAACAT TGATGACAGATACTATGACAAATTCTGCAGCTTATCATTCAGCAATTCTATATTGTTCCTTCAGACAGTTTATT CCTTCTTCGGAAACCATGCAAGCCACCTGGTTTGGATGATAAATTTGTTACTGTTGCTTTGGTGGTCACTTAGG CAATGCAGATGTCTGAAAGCCACTTCTTCGATTGAAGCAGTTGAGGGGGCACGAAATGGGCCTTATTGTGGAAG TCTCTTGATTTCTCTCATGTTGCAGGTTGGCTACTCTATATAATACAGTACTATGACAGTAAACATAAGAGGGC TTGATAGTGTGTGTGTGGCTTGGAGAACATGATTTCAAGAATTAAAATGAATGCGATTATGTTAGTAAGTTACA ATGTCAGTAAAGTGATGTGATGTGATTCATATGTTTGTCAAAGTTCTGAAAACCATACATACTATCAAAGCTCA AGTGATCTTCCACTTAAGGTATGAAGTGAAAAATAAGAAAGTCCAGTACGGATAAAATCAACAAATACAATATG GATCTTGCACTCAAGTGCTTTGTAAGAAAATATATGTATTGTTTTTTTTTTCAAAAAAAAAAAAAGTAAATCTC TGTAAAATCTACAAAAATGGAAGTATCCACTGTTAATACATTTTATAAAGCAGTTTTTCATTACATTTTACTCG GCTTACAATCAAAATACACAAATACCTTCCTTATTGTCTCATTTTACACCCCAAAAAAAAGCAAGCTCCTAAAC TCAATGCACTGAAAGTAGCTCCCCCGACAAGGAGGAAAGACAAAGAGATACCTATCCAGCAAAAATGAATAAAT AAAATTCAGAGGAGCCAATCCATGTTTCTATCTTGGCAAGAAATTAAGACAGAATAATTTGACAATATATTAGT GTTTCAAGCTTAAGTAAATTACAACCACATTGAACGTAAGGCAGAATAGGTTATAGAGAACATATAGCTCATGT ACAGTAAAATATTATTTAGAAACCTCTCCTAATAAAGCAAATCAAGTAACATTATCATTGATTTGATTCATAGT TAACACACAACATAATAT G GT G CATAAGC C TAAAT T TAAT T CAAT T TAT TAT GAG CAAACACAC CAT C CATAAC AAAATAATAATAATAAGAGTATATGACTCTCCCATGAAAGTTTTCCAAACATATCTTTCGATGATTATGCTAAA T TAACACAACAAATATAC C CAAATAAAGT T T GAT GT CAAGAAC GAAACT T CAAT CT CT GCACAT T GT T TAATAA AGAATTGCCTTCCATTACCACATTTTCTCCCTAAAATTTACACTTTACAAACTTCAAGTACAACCCGTCTGTGT AATTGATTCCTAAACAGTCCTTACGCACCAAAATCAATGAAAACCCAACAAAATTATAGTCAGAAGAAAATAAG ACTTACAAGTAAGTATAAAGAAGGCGCTCATATTAAGGGGAGTTTAGGAATTTGGCCTTTTCTTAGGAGAGAAA GAGTGTGCTCAGGTACATCAATAAGATCGGCAATATTGTGGAGTGATCGCTCTTCCGAAGCCACTAAGAATATG GATCGAGCCTCCGCCTTCTTCGACAACCGTGCGGCCCAAGTAAGTGGCAGCTCCGGCGAGTTGAGGCTTCGCAA TATTTGACGATACAGAGACAGAACACGTCCCTTGTTCCGGGCCAAATCCTCCGCCGTCGCCCATATCAAACCTT TCGCCATTGTCCTCTCAAGTCTTAACCAAGCTAATTTTCAAACCAAAGAAGAATAAAAATCAACCGAAATAATA TATGGAAAAAATCTAACCAAACAAAAAAATTGGAGAAAAGTTTCACTTAATTCAATCTAATTTAATTTGCAAGA T GAGAT GT AT T AGATT AAAT GT T GAT AGACAAT AAT T AGGT TAT GAT GT T AAGT GT AAT AT AAT AT AT AT AT AT ATATTTATTTAATTTTTTTTATTAGGTAATATATTCTTTTAATTTTTGAAATAGAGTTCGAGATTTTGATCTTT AAGAAAAAAAATTTAATTATTGGTACATTAGTTATGTATATATTGTAATTTATTATATTCTTAACCAAAACTCA CATAATTAAATTTGAGTATGTTTATATATAGATCGAATTTATAAATTTGATATAAAGATTTTTTATTCTCAAAC T T TAC GAAAAAATATAGT GTAT GAAT CAGT TTAC CATAAATAAAACAAAAAATAATCAATT C CT TAT TACAAC C AT T T CAAT T T TAAATATAAAATAAATAATAATAAAAT TAAAAATAT T TATAATAT T T TAAAT T TAAAT T T C T T T ATATAAAATAT CTACATAAT TTTTCTTTTTATTT TAAT T TAAAGT TAAAC CAATAAAT T TAAAAATAT GAT TAA AAATATTTATAATATTTTAACTATTTTAAATTAAAATTTCTTTATACAAAATAATTATATAATTTTTTATTTTT AAAT AT AT AT AT AT AT AAAAAAAT AT TAG AAAAT T AAT AAAGAAT AT TCCTATTTTCT T AAAAT T AAC GT AAAA CCCAAAAAACATCGTTAAACTAAAAATTCTGTTATATAGCCACATTTTATATAGAATAGATATAAATATATATT TTAAAAGTTATATATTGAGAATCATGGGTTCCATCTCAAAACCAATTAGTGATGAGTGGAGTAGTCCATGTTCT TATATATAGCTCAATACTTTAACATTTAATCTATGTGGGACAATGTTCTCAAATATTCCCCTCAAGATGGTGAT TATTTTTGCTCACTAATCTTGAATCACCTTATCACAAGTTATGCCATTTGCTCGCTTATTTTTATTCGGATCTC AAACGCTTTCAGACTATCTCGGATCTCGTATACCGGGCTAACCATCCTGGACGGCAGGGATTGAAGCCCGCTAG GAATGTGGCTCTGATACCATGTTGCGAATCATAGGGTTCCATCTAAAAACCAATTGGTGATGAGTGGAGTAGTC CATATAATTATATATGGCTCAATACTTTTACATTTAGTCCAAGTGGGACAATGTTCTCTAATATTATATATACA TGTAGAAAATAATAATTTTAAAATCTAATATATATTAAATATTTTTTATGTATGTTACGTTATGTTTTTGATTA AATAAGTAAAATTTTAATTAATCATGTTTTTTAATAATCTCGTTGATTAGCAACGAGTTGCACAGTACGTTAAA AATCACGTTAACATGCCTAAGTTACTTATGGTGTTACAATTTGGTATCAGAGCTGCCAGGTTGTCTTCCGAAGA TCGTCATGACATGTACAATCATCATCAGCGATCAGCTCGTTTCACGGTTCAATAAGCTTTTATTGCTTTAGTAG T T TAT T T TAAT T GTAT GACT TAAGAAAAGT C CATAC T GAAGCAT GT TAGAGCAC GATAAGTAGAATAAAGAT GT TTTAATTTCCAAATAAGCAATTAGTAAGTTTTACTTGATCACGATCCGACCGGACTAGCTCTTAATTCACGCAG GTCGTTCTAATAAGATGGATGTCTGCCGAAACACCGGAGCCGTGGTAACTTCGGTTGATCTAGCAGTGGTCAAG GAGTTCGATTCCCTAAATATTTCATACCGAGGTAGAGGTCCCAGAGGCGTGGCATGCTACCGGTGATATTAACC CGCCAGTCGCCTAATCTGCCAGTCAGCCGTTAAGATTAGGAAAATAGGTTTGTAGAAATGCAAGCCAGGATTAA TGAACAAGATGAAGAGATTTGACGCCTTAGACAACAGGGTGCTCCTAATCAATCCCAGCAAAGAAGCAGCAAGT ACCTCTGCCTTTATCAGGTAGGCGTGGACCACATTCTGAATGAACCAATGTAACAATTGTATGAAAGGTTCCGC AAGCAGGCACCTCCATTTTTCCTGGGAGGTCCTGATGTTATGAAAGTCGAGCAGTGGCTAACAGTGATTACCAA AATTCTTAACTTTATGGGTGTCACCGACAATGATAGAGTGGCATGTGCCACATTCCAATTCTAAGAAGACGCTC TAGTGTGGTGGGACATGGTGTCCCTGACTCATGACGTTACTACAATGACCTGGGAAAAGTTCTAAGAATTGTTT AATGCAAAATATTACATCGAAGCAGTTAGAAGCGCAAAAAGGAAA Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 65758028 65763027 1 5000 upstream regulator}’ region gene 65763028 65765653 5001 7626 gene biotype=protein coding 5kbs downstream 65765654 65770653 7627 12626 downstream regulatory region mRNA 65763028 65765653 5001 7626 gene=LOCl 15713370 exon 65763028 65763161 5001 5134 product=transcription factor bHLH91%2C transcript variant X2 exon 65763480 65764558 5453 6531 product=transcription factor bHLH91%2C transcript variant X2 exon 65764689 65765090 6662 7063 product=transcription factor bHLH91 %2C transcript variant X2 exon 65765205 65765653 7178 7626 product=transcription factor bHLH91%2C transcript variant X2 CDS 65763566 65764558 5539 6531 gene=LOCl 15713370 CDS 65764689 65765090 6662 7063 gene=LOCl 15713370 CDS 65765205 65765315 7178 7288 gene=LOCl 15713370 Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 65758028 65763027 1 5000 upstream regulatory region mRNA 65763028 65765653 5001 7626 gene=LOCl 15713370 5kbs downstream 65765654 65770653 7627 12626 downstream regulatory region exon 65763028 65763161 5001 5134 product=transcription factor bHLH91%2C transcript variant XI exon 65763483 65764558 5456 6531 product=transcription factor bHLH91%2C transcript variant XI exon 65764689 65765090 6662 7063 product=transcription factor bHLH91%2C transcript variant XI exon 65765205 65765653 7178 7626 product=transcription factor bHLH91%2C transcript variant X1 CDS 65763566 65764558 5539 6531 gene=LOCl 15713370 CDS 65764689 65765090 6662 7063 gene=LOCl 15713370 CDS 65765205 65765315 7178 7288 gene=LOCl 15713370 Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 65758187 65763186 1 5000 upstream regulatory region mRNA 65763187 65765653 5001 7467 gene=LOCl 15713370 5kbs downstream 65765654 65770653 7468 12467 downstream regulatory region exon 65763187 65763266 5001 5080 product=transcription factor bHLH91%2C exon 65763483 65764558 5297 6372 protais<4ffl^^^ factor bHLH91%2C transcript variant X3 exon 65764689 65765090 6503 6904 product=transcription factor bHLH91%2C transcript variant X3 exon 65765205 65765653 7019 7467 product=transcription factor bHLH91%2C transcript variant X3 CDS 65763566 65764558 5380 6372 gene=LOCl 15713370 CDS 65764689 65765090 6503 6904 gene=LOCl 15713370 CDS 65765205 65765315 7019 7129 gene=LOCl 15713370 Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 65758196 65763195 1 5000 upstream regulatory region mRNA 65763196 65765653 5001 7458 gene=LOCl 15713370 5kbs downstream 65765654 65770653 7459 12458 downstream regulatory region exon 65763196 65763266 5001 5071 product=transcription factor bHLH91%2C transcript variant X4 exon 65763480 65764558 5285 6363 product=transcription factor bHLH91%2C transcript variant X4 exon 65764689 65765090 6494 6895 product=transcription factor bHLH91%2C transcript variant X4 exon 65765205 65765653 7010 7458 product=transcription factor bHLH91 %2C transcript variant X4 CDS 65763566 65764558 5371 6363 gene=LOCl 15713370 CDS 65764689 65765090 6494 6895 gene=LOCl 15713370 CDS 65765205 65765315 7010 7120 gene=LOCl 15713370 Form Start End Extracted Scq-Start Extracted Seq-End Gene Name 5kbs upstream 65758307 65763306 1 5000 upstream regulatory region mRNA 65763307 65765653 5001 7347 gene=LOCl 15713370 5kbs downstream 65765654 65770653 7348 12347 downstream regulatory region exon 65763307 65763362 5001 5056 product=transcription factor bHLH91%2C exon 65763483 65764558 5177 6252 profeiseffl^sm^ factor bHLH91%2C transcript variant X5 exon 65764689 65765090 6383 6784 product=transcription factor bHLH91%2C transcript variant X5 exon 65765205 65765653 6899 7347 product=transcription factor bHLH91%2C transcript variant X5 CDS 65763566 65764558 5260 6252 gene=LOCl 15713370 CDS 65764689 65765090 6383 6784 gene=LOCl 15713370 CDS 65765205 65765315 6899 7009 gene=LOCl 15713370 >bHLH91 amino acid sequence from CBDRx (SEQ ID NO:10) MYMDNGSCSFDPIDTAEATAVGPEDAFNSQLPPPPPPLPSMTGFSAADEITSAAEELASAVEEFAYHQHQHQHQ HQHHHLLPNSQEEAAINMELQQQLAFNMDNSYNTDINTTAQLVSFDHLQHHNHHHHQQQPQWDNEDMSYNNNNH RHNQHHQEPADLLSLFHLPRCSATSSSSITFTNPTPPKTANFPNTSLAFLGDVPMGTDSPPSSSILYDPLLHLN LPPQPPLFKDLFQPLPNGYAFPSSSSRNGSLFSNGVMDMDGEGSGGVYQDGLEGMQFESGVLEFARDRSYIDRG RDGRGTKHFASERHRRVQMSDKYHVLKSLVPNPTKNDRASWGDAIEYIRELRRTVNELKILVEKKRRGIEANK RRKSEEDTAGEVENCNIKPEPDHSYIRSSWLQRKSKDTEVDVRIIDDEVTIKLVQRKKINLLLTASRVLDELQL DLRHVAGGHVGDTYSFLFNTKIYEGSSLYASAIANKLMDVLDRQFGVILPLPPTNSY See APPENDIX B for the four most common genotype sequences in Cannabis accessions for bHLH91. Gene name: TM6 CBDRx assembly gene ID: LOCI 15714657 Number of Isoforms: 1 Number of Indels: 61 Number of Exon SNPs: 82 Description of variation: For ~20 individuals, the genomic region of TM6 contains a large -4,500 bp insertion in the middle of the gene between exon four and exon 5. The insertion sequence of the insertion is similar for these 20 individuals. The CBDRx annotation is very similar to all other individuals. >TM6 nucleic acid sequence from CBDRx (SEQ ID NO:3) CTTTGAAGATAAACATTCGAAAAGAAAGTTCTAGATCTCTCTTTTAACTTTTCAAAATATCTTGAATCGTTTAA TTTTGAGCTATATCGAAAGAGTTATGACTAAAATATCATCAGTCGTTGTAGCAGGATTTCAGAATTACAACAAT TTTGTAAAAAAAAATCCAAGAGCAGCGACTCTCTATCAACAGTCGCAACTCTTGAATATTTTTGGGAGTTCTTG TTTCGACCTTAAAACGAGTACGGAGGGTAGATTTTCATCTAAAAGTCAACTTTAAAGACAAAAAGGAAGCAAGA AAGGACGGCTGGATATAAGAATTACTATTGGAGGAGTAAGTGTTCTTTTTCATCTTTTTATCTTTTATGTTTTT AATTGAATATTTGGATGTTTAAGATGATTGTTATTGGCTAAATCTTTTAAGGGGTGTTATGTGATTCTCTTAAA ACTCTTTTTATGGATTAAAGTAGAATTTATTTTCTTCTTCATCTCTTATTCAATTTCGTACAATCTGTTTTATC GTTCTAATTATTTACTGGCTGTTAGGCTATTTTTAGCTTACGTTTTGGGTCACTTTTTATAGTTGTTTTCCTTC TTTGTTATTCTTTTTGGAATAGAATTATGCTTGTTTTCTTTGGTTTCAGGTTTCACAATTGGAGTGTATAAATT GATTAAATTTCGGAAAACAACAATCTATAGAATAGAGAAAATTCTGCAAGAAAATAAAGCTGAAACTTCAAGCT TATATTTGACTATGTTCTGATCAGATTTTGGAAAAAGTCAAAACATAGAAGTTTTTAATTTCTTTCCAACGCAT CTTGAATCAACTCATTTGGAGTTCGTATGTGAAAGTTATGCACATTTTACTGAAAGATGCTGAAGTTGTCAAAT TTGCTTCAGTAGTCGCGGCCACCACTTTCCATGGTCGCGACCCTTTTTCATGGTCGCGCCTAGGAGGCCAGACA GCAAGTTTATTTTTGTATTTTGCCTATGGTCCCGACTAGGCTTTATGTAGTCGTGACAGGAGCGAAATACGAGA TTTTTGGCCAATTTTTGACTTTTTCAAAGGTAGCAACCCTAATTTCTATATAAGGGGCTCATGTTTTAGAAGGA AGGGAGGCAGAAATTGGACCCAAAAGTGAAAGAAGGAGGCAGAGGAAGCAATGGTGGCGATTCATAATGCAATT ACCAACTAGTTCTTTCTTAAACTTTTAATTTCTATGTTAATTTCTGTTTTAGTTTTGATTATGGATGTTATTAT GGATTTTATGAACAAAACTCCTATTTAGGGAGATGATGAATGTTGATTGAAATTACTTTTGGTTAATGATAATT GCTATTTTTCCTTTCTTGATTGTGAATTTATCCATATTTGTGTTTAATTACATGTTTAAGATTGATCACCTTCT ACAT GTT CTAT GAT CCTAATT CAAAAT CT GAAAAGT GAGAATT GGGAAT GCTAATAAAATT GGATAAGCTAGGT T T T GAT GT GAAAC GAAAGTAT T T T CATAGC CAT T GT GACAT T TAGATTAT T GCT TAATACT GAT TAT AT GT TAA TCTATCTCAGAGTTGATTAGATGCATGTGATTTAGGACCTAAAAGATCTGAAAAGAGTTAGGTTACTTTTTATA ATCTGTCATTCACATTAAGAAAGAGAATAGTAATTAGCATTAACAATTGGGTAATTAAATCAACATGATTCTTT TTCCAAATTTCCCATCCTTGATTAAACACCTTTATTGCTTTCTTTAATTTTCTTGTTTATCATTATTTCAAAAT CTATCAAAATTATTTATCTATACCACTACTACAAAAAGGAGTATTTTCGTCGGTTTTCAAGTGACTAATAAAGA TTTTACGTCGGTTTAAAATGACACAAAAAACTATTTTCGTCATTTTATAACCGACGCAAAACACAAAAAATATT AAACATTTAGCGTCAGTTTTGAAGGGACGCAACAAGCTATGCGTCAATCTTTAACCGACGCAACAAGTATTTTT TGCGTCAGTTCTAAAATGACACTTAAGTGTACACATAATATTTTTCACAATAAAACTTTCTACATATTAAAAAA AAT C AC AAAT AAAC T AAT AT TATTTTGTC C AT AT AT AAAAGAT AAG CT AAT AT CAT AT AT T C AT AT AT AAAC AT ATACATTATTATGGATTTTATGAACAAAACTCCTATTTAGGGAGATGATGAATGTTGATTGAAATTACTTTTGG TTAATGATAATTGCTATTTCTCCTTTCTTGATTGTGAATTTATCCATATTTGTGTTTAATTACATGTTTAAGAT TGATCACCTTCTACATGTTTTATGATCCTAATTCAAAATCTGAAAAGTGAGAATTAGGAATGCTAAAATTGGAT AAGCTAGGTTTTGATGTGAAACGAAAGTATTTTCATAGCCATTGTGACATTTAGATTATTGCTTAATGCTGATT ATATGTTAATCTATCTCAGAGTTGATTAGATGCATGTGATTTAGGACCTGAAAGATCTGAAAAGAGTTGGGTTA AT T T TTATAAT CT GT CAT T CACAT TAAGAAAGAGAATAGTAATTAGCAT TAACAAT T GGGTAAT TAAAT CAACA TGATTCTTTTCCCAAATTTCCCATCCTTGATTAAACACCTTTATTGCTTTCTTTAATTTTCTTGTTTATCTTTA T T T CAAAAT CTAT CAAAAT TAT TATAC CAAATAGAAT CATAAGT C TAAT TTAGTGGTATTCAGTT CAAT T C C C T GTGATTCGACCTCACCTGCGTGAGTTTACTACTTGATTACGTGCACTTGCGTATTGTCTATAAATTTCGTAACA CTGGCTATTTATATTTTTTTATTCATGAATCAAATTGAAATCTGAAAAGTGAAATTTAATATACTTTGATTGTT TGGATATACATTCCCTCTCTCTTAAAACTGTCAAAAATAACCTAAATACCGCTCATTCGCTCAAAACATTTCAA ATCGTCTCTCATTCGCTATCTCTCTCTCTCTCTACTATCACCTCTCTCTCAACCCATTTTCGATGACTGATAGT CCCAAATGAAGTACGTGTTTGAGATCTTCGACCCCCAAAAACTTTCATGAGGTAAATGTCTATCGACCTCATCT CATTCTCTCTTCTTCAACTTCTTATTCTTTTCTGTTTTTTAGATTCATCTTTTTCTTCTCTATTTTTATTTATT GTATGGATCTTGGGTTCTCTCTCTGTTCATTGAATCTTTGACCTTTTTTTTATTTGTCTACAGATTTGTGGTTG TTCTATGTTGGGGGCTCGCAAATGACCAAACTTGCGTCGAAGTTGGGATCCCAGTTGGTCGAAGCTGTAGATCG AACCCGACCAACTCTATAAATTTATTAATTTGAAACTTGAAATTATGTGTTTAGAATTTTTAATTTTGTGCATC TATTTCATGGATTTTGATTTGTTTTAGATCTGTATTTATTTAGTTTGATTTTGATAGATCTTAGGGCTCGTGTA GCTCATATCTGAATTTTTTTAATGTGATGATTTTGAACAATTGACAATTTCTTTGTTGGCTGTTCTAAATTGTC AACTATAATGCCAGTTATTACCGATAATTTTAAACAATTAGGACATAGACATACATGTGATTTTCTACCAATGT TTATACTATTTATTTTTGCGACGGCCGAACAGGCGTCGATCATAAAGATCAATGACATACCCAACTCTTAAAAA CATTAGAAAAATT CATTTTTTTTAGTAGT GAAAGCATTTAT GT CAT GAAAATAACCAAACTACATAACTAT GAT TTCACGAGTATCAATGTGGCAAGTGGCAACCAATTAAGTTAATAAATAAGTAATTGACTTATGAACCATTTTTG TTGAAACAGCCTCCACTTATTTAGACTGAAACACTAAGCATTTTTGTTGATTAAACAGTGGTTTTTTTGGAGAA GGAGCATGCGTATGCATATATATGGTTAAATATTATGGTACTATAATTTTCGATGGAAATTGACATATATATAT ATATGATGGTGTGAAGAGAGCTATACATAACCCGGAAACTTTAATGATGAGACTATTCAAAATTAATTGTGATC ATAGAGATCGAGATTACATGTGTGTGTGTGTGTGTGAGCGCTTATTGATTATTACAACGTTAATCTCTTCGAAA AC TAAT GAAAAT CAGTGTTTC GAT GGT TAAGATATAG C C TATAAT T G GAAC TATATATATATATATACACACAC ACATACTCGCGGTAATCTGTAACTTACTAATAGGTTTTCGTAATTATGACACAACTGTAACCAGATTGATAAAA CCATTTTTATTATTAATTAGTATATAGTTAATTAATGAGCATTCTTATTAGGTACCGGTGGTGTCTAATACTTT CAAGACACGTTTCATTGTGATTGGTTAACGATACTTCAAAATCATTACATTAATAATTCGGCCAGTTGATATTT AAC G GTAATAT TCAGCGACCTC CAAT T TACAAAGAAAAGTAAACATAAAAG GATAAT T T TAC CAAAAAAAAAAG AAAAT TAAGT GTAAAAAT CAAAC CATAAAAAAAT T T CAC CAAAAATAAC C C T TAAATGC GTATATAT T T T GTAT ATTTATATAGAACTAAAGTTCAATTAATAAACTAATTAAGCTTGATTAACTCTAACAATAATTAGCTTCAGTAC TATATATATACCTAATAAAACAGTACTACTTATATGACATATATATGAATGAAAGACAAGACCAGGCAAGGCAA CTATGCCTCAGCGTATCGAAGTACCTAATCCATCCGATTATCCAAACGAGTTAATCACAGCCATTCATTATCCA CTAC C CAAAAACAAAAAGTAAACATATAATAC GTAC GTACATACAT TAATATAGT TAG GACT CATATACATATA TAAAATACAGCTCACAAAAAAGAATAATTATATATATTACATTCTTGGTTAGACTACAACCCAACTTTCTAACT CCCATTTCTCTATATAGAAACCCTTTTGGTTTAAAACCATTATAATCTTCTTCATCTTGAAACCCTAGATCTAT CTAT CT CTAT CTAT CT GTAACCAT GAT GAGTGGTTATAAAT CT GAGCCAAACAACAATATT GAT CAAGACACT C AT CAT CAATAT CAT TAT CAT CAT CAT CATAAT CAT CAT C GT GAT CAT GAG GAG GAAGAAGAAGAAGAT GAT CAT CAT GAT GAAAACAGCAGCAATAAGCAACAAAAAATAGGAAGGGGAAAGACT CAGATAAAGTT CATAGAGAACCA AACAAACAGACAAGTAACCTTTTCAAAACGAAGAAATGGGATTTTCAAAAAGGCTGGTGAACTCTCTGTTCTTT GTGATGCTAAGGTCTCTCTTATTATTGTCCCTAACAATAATAAACTCCATGAATTCATTACTCCTGGAGCCAAG T AAGAAT AT T TAT AT AT AT AT AT AT AT AT TAT T AGAAAAGT T T AGAT C T T T AT C AAT TAAT T T C T GAT AT AT GT GT TAT CTTTT CAT GGAAT CAGTACTAAGGAAAT GAT T GAT CT GTAT CAAAGAACCAAAGGT GTT GAT CT GT GGA AGTCACAGTACGAGGTTATTAATTATTTTTTTTTTATTAAAAAAAATCTGAAAGTTGTTTTTTTTTCTTGATTA TTTTTTATTTATTTTATTTTTTGGTTGTAGGCAATGCAACAAACCTTGCGCCAACTCAAAGCTAAAAACTTTGA AC T GAG GACACAGAT CAGGTATTTTT TAAAATAT TATTTTTCC TATATATATAAAAAAG GAAATAAGAGAGAAA AAAAAAGTGACACATAAATGATTTTTTATTTTATTTTTTGTAGTTTGATGAAATTAATATGTTTTTTTTTTTTT CTTGAAACAGACAGAGATTGGGTTATGATTTGAATGATCTGAGTTTTCATGAGCTAAGTGATCTTGAGAATAGT ATGCTTTCTTCTGTGGAAGCCATACGCCACAGAAAGGTATAATTTATAAATCATTATATTTTCACTATTTTTTT TTAAATTATTTATTTCCATATTTATGTTTGAATCATTAATATAGAGTAATTTGTGGCATAAATACTTAAATTTA ATTCCAGTTGCAAATAAATACCTAAGTTTAATTTTTTTGGCAGTAATAATAGCTAAGTTATAAGATGTCAAAAT T TAAT GACTAGT CAGTAAAAGTAT T CAAAAAAAAT T GT GAT T T TAGATAT T T C GT GTAAAT T TT CCAATAAATA TATATACTGTTATCTCATTTGATCGAGTATAGTACTAGATTTCATTATTATCGATCATATACATGTTGGAAAAA TTCTCATTACAAGAGAGAATAGGAGGATTACAATCAAAATACTCAAAATACAAATAAAAGTATTTCATCAAGAT TACAAGAAATCTAGAAAAGAAGAAGGATAACACACAAAAAGCTTGACCAAGGCTCAAGGACCTTTGTCCTAAAA GTCATTTCCTCCCTCATATGGACACTTAGGGAAACTCTAGAAATGCTCTTAAGGATTTATCAAGCCTCATATGC TCAGCCTAGTGTCTAAGGATGAGCATATCCTCACAAGGTTCTAACCACAACAAATGGTTTCAACCACTTCTTAT GCAAGTGAACAATATGGCCTCTATTTATAGAGTTTTTACTCTCATATGAACATGAATGATCACCATAAAGCCCT TGGATGTTACCAATCATAAATTGGGCATTTATGTTATTAATTGGGTGATTTGGAGGAGTTTTGGGCTGTTACAA AGGCTCTCAAACTTTCAAAAACGTGTGCAATAATATGCTGAAAAAAATAGTAACTAGTTACTAGTTACCAATTT TTCAGCTTTGGCCAATTCTTCTCCAAAGTGTTCCAAATCATTCCCACTTGATTTTGAACCATTTATACACCTTA TAAAT GAATAAAT CAAGT C T C CAACAACTATATT T T TAATAGCTAT CAT T CAT T CACAT T CATAAATAAT TAGT AACAT CT T TTACTAAT T TAAGAGT GAAAAAGAGATGAGAGT TAGATAT T CAAGT GAT CAT CAAT TTAAAGGAT T TGCAACTCCTATTTTGTGATCATTCTACATACATTTTGTAACTCCATAATGTATGTTACAATTTGACAAAATTA ATACTTTGTCACACTTAATTATTTACAACTTAATTATTTAATCTAAATATTATATTATATAAAATAATATAACA TTCCCCCACTAGATTAAATAATATCTCATTTGTAGCAAATAATTTTGTAACACAACATTTTATTTAATCAATCA AACATTATATTATGGAGTAATATAATATTCCCCCACTTGATTAAATAACTTCACTCTAATAGAAACCATTGCAT TTGTGCATAGTGTAAAATGTCTTTCGACTTGAATTTTACCTTAGTGTAATTACTACAAAGTTTATCGAAATTTT GGTTGCCGTAGCATTGAACCACTATTTCTTGATAACAAACCGATGATAACACACACACATTTGCTATGTTCACT CAAGAACCTTATGGATTGCTTTTGCACCGTTAATGGCCATGTGCACAAATCCAATTCATGGACTTTCTAGAGAA TAACTCCCAATTCTCATAAGAGGCGGCACCACCCCTAGTCCATATAGGTGGAGTTTTAGTGTCTCACAACACTT TAGACACTTTCTTAAGCTTAAGTGAGTTTTCTCAAGCTTAAGCTCCATTAAAAAACCTTTCAGTTTTAACCATC AATTTTCACCACATGTACTCATCCATAGATGGGACAATTGAGTACCACATTCACTCTATTGACTTGTTATTACC TATTGAACTTAAGACTAGATTTTTACTAGTGTTAAGATAGGTTACCATCAATGAGCAAAACGCTAAGGGAGTTT AGGTCCCATCCCTCGAAATTCATGCTTTAATCTTGTATGAAGATTAAGCGATTTGTTAACCTCACACTTTTGAT TCCAAACGAATCATGCTAGCCAAATATATAGTGTTCACTTTGTGACCACTTTTCTCCTTAAGTACTTAAGCTTT GAAAAT T CAAT CATAAAAGAT TAAT T T T GACACAAC T CAAAT T CT CAATAAT T T T GATAT CAAG CATAT CAAAA T TAAACAT TAAT T TAGACAC CAAG CAT GT C TAAAT TACACACTTAACTT TACACAT CAAACAT GT CAAAGT TAT ACTATATTTAAGACATCAAGCATATATTTCAAATATATACATTTTTAGTTTTAGACACTCAATATGTCTATACT TCTCATATTGGAGTATGCACCCCCACTATCTTAAATTTAATTTCCTTCTTGAAATTATTACCACCAAATTTTGA C AC T AAAT AT GT C AAAAT T T C AC AT AT GC AC AT AG C AAAAT AT TAAT T T AGAAT AT T AAAT T C AAAAC C AC AT A TATATAATCAATAATTTCTCAATAAATTTGATTAAACTCAAATCTCACCCCCACATTTAACTATAAAGTTAAAA TACCACATATCACTTTAAAATAAATCTCTTTATTTTATTTTGTTCTCTACACATTATCTTTCAAAATAATGTGC ACTCAAAATTATCATGAACCTTTCGATATCACATGATAATCTCTCCAAATACCACTTACAAATTAATAGAATAT TTTATCCATTATTTGTCCTTTATTAACAAAATTATACTTAAAATTTGTTAATAGATAACACAAAATTCACATAA TGATAAAATTGCTTCTTCAATTTTATGGTTATCTCCTCATTGAGATTAGTCCAAAGTTAACAAAATTACTTCTC ATCAATTTTGTTTCACCACTTTACTTTTTAAGATTCAAAATTGCTTCTCCAATTTTATAATCTTTTATCTCATT ATTTGAATCCACAATGATCCACTTTCTTTGAGTCTAAAATTGTCCTTCCAATTGTATGAGCTCATTTTACAATT TAGACCCACTTTGATCACTTTGCTCTTGCTATGAAATGAACAAACACAAAAAAGTGTAACTCACATAGTTCACT TTTTCTTATCTCATAAAATGAGATTATTATCACCAAAATGGAACTTCATTTCCAAGTAACTATCTTTAATCACA ATATAATCTCATAATAATAATGATAGAATGAACACATTTTCTTTCTCATCATCTCCATTATCATACCATATAGT AC AT AT AT T AAT AT AAT AAT T TAT AT AT AT AT AT AT T AAT AC AC AAAAT T AAT AT AT AACT C C C AT AC AAT TAT AACTCTCAAATAATTTTATGTTAATTAATATTGTATATATGTTACCATACTATCATAAATAACATATACAAAAT AT C AAT AT T AAT T C C AC AAT AAAT AT AT AT AT GT T AC AT AT T AT AT AT AT AT AAC AT AC AAAT ACAAT AAT AT G C AT AT AC AC AAT AT AT T T AT CAT GT AT GC AT GT AT AT AGAAAT C AC TAT T T C T AC AT AT T TAT TAT C AC AAAT A TATATATATGTAATTATAT CT CAT GCT CAT CATATAATATACATATAACT CACATAT GT CACATATTATAT GAT ATATATATATATCT CT CT CACATATATACATATAAT AATAT GTTATAAT CT CAATTATAATATTATATAT CACT AT AT AT AAT AAT AT AAT AT AC AC AT AT CAT AT AT AT T AAC AT GT AAAAAC T T T AT AC C AAAAT AT CAT AT C T C A T CAT AT GAAT AT GT T AAT AT AT AT T AC TAT T AAAT AAAT AAGAT AT CAC AAAAT AAAT C C AC CAT GCTTACCCA TGGATGGCTTGAATCTCTTACCACATTATTTTTCTTCTCATGATCTTGATTTATGTTCTCCCTCATGGATAATT TTCTTTGATTGTTTGACCTCCATGACATGTCTTTTAGAAAGAAGAATATGCCATATAATTGATTTTTAGATTGT TAGAAAAAAT T CT CAT TACAAGAGAGAATAG GAG GAT TACAAT CAAAATAC T CAAAATACAAATAAAAGTAT T T CATCAAGATTACAAGAAATCTAGAAAAGAAGAAGGATAACACACAAAAAGCTTGACCAAGGCTCAAGGACCTTT GTCCTAAAAGTCATTTCCTCCCTCATATGGACACTTAGGGAAACTCTAGAAATGCTCTTAAGGATTTATCAAGC CTCATATGCTCAGCCTAGTGTCTAAGGATGAGCATATCCTCACAAGGTTCTAACCACAACAAATGGTTTCAACC ACTTCTTATGCAAGTGAACAATATGGCCTCTATTTATAGAGTTTTTACTCTCATATGAACATGAATGATCACCA TAAAGCCCTTGGATGTTACCAATCATAAATTGGGCATTTATGTTATTAATTGGGTGATTTGGAGGAGTTTTGGG CTGTTACAAAGGCTCTCAAACTTTCAAAAACGTGTGCAATAATATGCTGAAAAAAATAGTAACTAGTTACTAGT TACCAATTTTTCAGCTTTGGCCAATTCTTCTCCAAAGTGTTCCAAATCATTCCCACTTGATTTTGAACCATTTA TACACCTTATAAATGAATAAATCAAGTCTCCAACAACTATATTTTTAATAGCTATCATTCATTCACATTCATAA AT AAT T AGT AACAT CT T T TACT AAT T T AAGAGT GAAAAAGAGAT GAGAGT T ACAT AT T CAAGT GAT CAT CAAT T TAAAGGATTTGCAACTCCTATTTTGTGATCATTCTACATACATTTTGTAACTCCATAATGTATGTTACAATTTG ACAAAATTAATACTTTGTCACACTTAATTATTTACAACTTAATTATTTAATCTAAATATTATATTATATAAAAT AATATAACAATACATATAAGCTAAAACTTATAGTACAGAGCACTTGCTATTTGACACCTAAAGGTGTTCAAAAT CATACTAATATAATTTTTTATAATTAATTAGCCATTCTTCATATTATTTATTATATATCTGAGACTCAATATTA AAATAGAGTAATAGTATATATATCAACTTTGACACCTAAGGTGCAGCTTAGCATTTCTTATATAATAAATCATT TGATAATTAATTGAAATTAAATTAGAACGTGCATCGTACTAATTCTCTTGTTCATTATGTTTTTCAGAATCACA AGATCAAAACTCAGGCTGAAACCACCAAGAAAAGGGTTAGAACACACACACTGCATATTTAATATCAACCTGAA TATAGTCTTTTCATTTCTATTTCAAATTAATTTTAATTAGTATTTATATATATAATATATATATATATGTGCAC CCTTTAAATTGTATATATCACCATACATGTTTTTTTTTCTAACAGAACAAGAGCTGGGAGGAAAGAAACTCAGT CCTCATGCATGAAATTGTAAGCTATAATTAACCTTATAATTAATTAGGTACATCTTATTAATTATAACTATACA AT AT T AGAT AT AGACT GT TAT AT TAT C CT AAAT AT C T AT AT AAACATT AT GT ACACAGT T AT TT TAAT T AACAT AT CATAT GAT T T GT TATAAT GAAATAAAAT GGAAATATAATAAAT CAATAT T TAT T T TATTAAT CTGTTGGTTG CGTGTTTTAAAGTATTAGAATAATGACTATTCCATATTTACATCCCAATTATGCAAGATAATATATATTTCATT TCCACTTCTATTTTTAATTAATCTAATTAATGAAAGTGTGGTAATTAATTAATTGTATTATTGTTATTATTAGC ATGCAAGGTGTGAAGATCCACCATATGGTTTAGTGGAGGATAATGTAGAAGGAGGAGGAGGAGGAGGGTATGAA TCATCAGCAGTTGCATTAGCTAATGGAGCTTCCAATCTCTATGCCTTCCGCCACCACCACAACGGCGGAGGAAG CTTAATCACCACCAGTCACTCCGCTCTTCACAGTGGCACCGGCGGTGGTGGTAGTGGCGGTGCCTATGAATCCT TTGATCTCCGCGACCTTCGCCTGGCTTGATGAATTAATCTGCACCCTAATCATATGTTAGTACTACTAATTATA TATATAATTAATATGTGATCAGAGCTTGGAGTGCATGCATTTATAACTCTATCATAAGAATATTATGTTTAGTA TGTACTACTTTTGGTTTTAATTTGCTTGACTTTGGTAATATATACCTTAAGCTTCTGATGATGATGATTTATAT ATTAATTATATGTTATGATATACTAAATTAAATGTCTCTGGTTTGAATTTACTTAATTATGTATATTCCTAGTT TGTCTAATTTTGTTTTGATATGTTCCATTGATGGGAATGGAGACTTAATTATTTATGACTATTTATTATTGATT TGAATTTAACAAGTGTAGTGCATATTGCATAAATACATAGATACACAAACTACATGAGCCTTTGGTCAAGTAGC CATAGAAGGTATTTGGGGATTGTGTGGGGTTATGTGATCCCACTGCAGGAATTATGATCTTTAGGGGCGATAAA AGTTATCCTTAAGAAGTAGCTCCTAATAGTTTGCATCTAAAAATATTATAAGGGACAATAAAAAAAATTATCAC CCCTAAAAGTAATTGATAAACAATTAAGGTTTACTTTTAAGAGCGATAAATATCATCTTTAAAACTTTACAATT AGTTGAAAAACAATTTTAAACAATATATTGATATTGCTCCTAAAGAAAAAACAACAAAAATACAGTTTTACGGA ATTTTAAACATTTTTACGATTTTTTTTTTATTTTATTTACATAAAATACGGTCGTTGAAATTTTGTTCATTTGT TGTTAATTTTTTGTTATATGTATGTTATTTTTTGTTGTTTTTTTGTTATTATTTTGATGTTATTTTCATGTTAC TTTTATGTTGTTTTTTTGTTGATTTTATGTTGTTTTCGTGTTATTTTTTAGAAAACCGTAAAAATGTAAAAAAA ACAT T CT T T GAAC GTAAAAAT GTAAACAT T TTACAAAAAATAGT GCAT TAT GTAAT TAT T C CAAAAT TAAAAT T ATTATAGTGTGATATCAAATCCAAAATTTGTGTAATTCGGCCTCAATGTATATATAAAAATGCTTATTATATAA ATAAATAAAACAACATAGATAGTAACCATGTTGTTTATCAAATACTTTCGGGTTTTTTTTTTTTGGAAAAAAAC AGAAAAATACAAAAAAAAGGAAAAAAAATTACAAAAATACTTTGGGCCGGCCCATTAAACATTTATACAGTCCA CATACAAATATTTACAAAAATACCACATGCACTAAGCCTCACTATACTGAGGCGAACCATGAAGATGAAAATAC GCGCTCGTTTCAAAACCGCAAAACAACCAAAATGAAACCAAAACGAGAAAAATACAACTATTAATTCTGGCAAA AT CAACT GGAAAAGAAGACCT GCAAT GAT CTAAACT GAAAAT GACGAAAAAAAAAT CAGAAAAACTGT GAAGAA ACTGAAATTACACATTTGTTTTCTGTTTTATTCGATCAAAACAACTCCCCCTAATATCGAAATCCAGATTCATG AAAT GTAGAT CT GTAACAAACAGAT CT GGAGCT C C CAC CAAAT T CAAAACAAT GTAT GAT GT GAAAAT T T T TAA AAAAACCTCATGAATAATACATCTAACGTTATATCTGATTACATTTTGATTGATTACTGGTTTTCAATATAAAT ATAT T T T T T GAAAAATAAAATAAGAAGAGTAAAAT C GAAT TAAGGTACAAC CAAT TAT GTATAAGT CT GT T TAT TTTTTATTTTGGTTGCCTTATAATTGCATTATCGTTTTATGATAGTTTATATATTATGCAAGAATTTAATTTGA TGGGGACTAAGAAATAGTAGTTATACATAGTAAGTTTTGTGCAAAATATAGTTGCATATTAATTTTATAGTGAA ATAACATATGCAAGTAGCAAAACTATAATAAAACTACAAAATAACT GTAT GCAAGT GCAAATAGTTGCAT TATA GTTGCATTATCGTTTTATGATAGTTTATATATTATGCAAGAATTTAATTTGATGGGGACTAAGAAATAGTAGTT ATACATAGTAAGTTTTGTACAAATAGTTGCATATTAATTTTATAGTGAAATAACATATGCAAGTAGCAAAACTA TAATAAAACTACAAAACAACT GTATACAAACTAAAATACAGAGGAAAAACT CTTT GAACAT CAACAT CCAT GAT AAAT CT CATT GTTACCCATT GAT GATATAAAAT GGACAGAAACAACTAGATAAAAACATATTAAAAAATACAT C TGAAGGTGTAAAATTTCAAATATGGGAGAAAACTAAAAAGAAACCGAAAACAAACCTCGGTTCGAACATCAGCA CCAACATTAGCTTTTTTCCCAGAAACGTTGACAATGAAATCTCTGCATTATTTGTTGAGCAAACATACATAAGA AGCACAATGATTTCCTAAGATTGTGCCTAAAACCGATGAAAATATTTTCATTTCTCTAACACTTTCCTTGACTT GACTATTCTTTATTATTTGATTTTGTCTTGGTATTAGCTTTTGTTGTCTATACTTTTTCTGGCTTTATTCTCTA CACAGTCGCTCCATATGTTATCAAGGTCCGTTTCTTCGTAAGAATATATAAGAAACTAGCTTTACAATTAAAAA T TAAAAATAAAAAGAAAAGAAGAATT GTTAT GGAAAAAAATTAAAAAT TAAAAACT GAAATAAAATTAAAAAT T AAAAAT TAAAAAC T GAAATAAAAT TAAAAATAAAAAGAAAAGAAGAAAAAAAGAGAGT GAAAT GAT G GAT T GAT TGATAGAAGTCAATCCTAGACTCCTGACTTGTAAGCAACAAGTGTGATGCATGAAAATGTGTGTGCAAAGTGGT AAAAGT GTAATAAAATAAACT TGAT GAGTAAAAAAGTAACAATAAC CGTGT GAGAGTAT TT CAGTAATAAAGAA TGCATTTTGTATTTATGGTGTAAAAATTTCTTTTTTTTTTTCTAAGTAAGGATCAAATATTTAAGTTGAAATTT GTAATTAATATTTGTGTACATACTTAGTGATGAAAAAAAGAATAGAATAGCCTCTTTTCTTTTTCTAAGGATGT CTTACAATTTATACTTTCCTAAATTAAATGTATCTATTTTTAGCATCCAAATCAATTGAACAAGATATTTGAGC C C CATAGT TTATAT CTAAATAATAATATAGT CATAAAAAAAAAAT GCTTAAAGTAT T T TATAAATAAT T TATAT T T T GT TATAAAATAATATAAAATAATATAAAGTAGAT GAGAAGAAAGAAGAAGAAGAT GAAGAGAGAAAGAGAA AGTGAATGAGAATTTCTGAGTTGTTTATTCCAATGGGGTGAACCCCTATTTATACAAATACAAGAGTGAGATAT TAAGAAACTAAGAAAAAGGGAAACTAAGAAAAAGAGGAATGTTGATTACAATTAATGACAATAAATAAAAGATT TGGACATCCACATAATTAATAATATTTATAACACTCCCCCTTGGATGTCCATAATAGCTGCCTTATTAAAAATC TTGCTGAAAACTTGGTCAAAGGAAAAAGAGTATAGTGTAAATTAACTCCCTCCCCCTCATTTAGGCATTTGTGG AGATCTTCTAATCGACGAATTTCGATCTTGTCTGCCATCTTCTCAAATGTTGATGTTGGCAATAACTTTGTCAA TAAGT T T GTAAAAT T GACACT T GAT T GAATAT GT T GAACAC CAATAT GCAT T T T CT T GAAGC GTATAAAGAAGA ATTTCGTGAAATGTGTTCTAACTCTATCTCCTTCAATGTACCTCCTTTTAGTTGAACGATGCAAGCAATATTAT CCATAGAGAATTGCTTGGGTTGATACTTCTTTATTGAGTGCAATCCATATGTTTCCCGAATATGCTATGCCAAT GATCTCACTCACACACATTCTCTACTTGCTTCATAAAATGCAAGTTAACATGATTTATAGTTGCCTCGTTAAAA ACCTTGCCAGAAAAACCCAGTGGGACAAATTCTGAGCTAAGGGAAAAAGAGTACAATATATATTTCATATTCAT AACTATTTGCAAGTTGCCTCGTTAAAAACCTTGTCAGGAAAAACCAGTGGGACAAAACCTGAGCTAAGGGAAAA AAGAGTGCAACATGAATATGTCTCCCCCTCATGCACATATGATCCATAATTCTTTTGGTGATAATATATCTCAT AAGATTATTGTTATCACTTTTAAAATATCACCATATATAATCTTTGATCATATATTTTCTATAACTCTTCTAGA GTATGCATGGACTTCTAAATTATAGATGAGGGGTTCGTGGAACATTAGTCTTTATCCAACTAATTGTATCATTC AT GT GT GTACACAACT TT GT T CATAC GAAAAT T TAAAAT T T CAAGTAGATAT GAACATAACACATTAAT GGTAC TACAAATTTTCATTTGTGACAATGATGGTACTCCCAGACCATATATTTATTCCATCTTGTTTTATGATCTTAAT TTCCATATCAAATGATCATATATCTATAATTCTTGATACATATGAAGTACTTCAGGACTTCAATACTAATGAAC AAACTTTATACATTAATATTTCTCGATATACAAAAGAAATAAAAGTTTGTTCTTCAATTAATCAAAAACTCTTC AAGAGTCTATCACAACGTATAATTTACGTATTTATATTGCATAGACAACCATAGGTATTTTTTAAATAATAATT TACTTACATGTCTTTATTTCATACAAAGATCTTTGTCATATAGTGCGCGCGACTTTGAATTTATATCACTTAAG ACATGTATTAAATTCTTCTAGAATTTTTAGATAAGGCTTATTTCAAATTCTCACTATATTAGGAGCTCCAAATA AATAATCTTTTGTTGCAACATTTATGTGACGCTTATAAATCCTCATAAAATATATTCCAGGCTATAATCAAATA ATGATTATATTTTCTCAATATTTAAGCCTTACTTCAATCAATCTCATGTCTATGCAAACTTTGTACAACAATTC ATTATGTACAAATACGTATATGAAAATTTCTCATTAGAAATATTTATTTGCACACCCTACAGGTCTTACTTCAC TTTATGTGAGTAAATTTGCCTGGATTGCGTCATAATATTTTGGCCAAAAATCAAAAT Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 85333862 85338861 1 5000 upstream regulatory region gene 85338862 85345865 5001 12004 genebiotype = protein coding 5kbs downstream 85345866 85350865 12005 17004 downstream regulatory region mRNA 85338862 85345865 5001 12004 gene=LOCl 15714657 exon 85338862 85339263 5001 5402 product=agamous-like MADS-box protein TM6 exon 85339360 85339426 5499 5565 product=agamous-like MADS-box protein TM6 exon 85339517 85339578 5656 5717 product=agamous-like MADS-box protein TM6 exon 85339719 85339818 5858 5957 product=agamous-like MADS-box protein TM6 exon 85344882 85344923 11021 11062 product=agamous-like MADS-box protein TM6 exon 85345082 85345126 11221 11265 product=agamous-like MADS-box protein TM6 exon 85345480 85345865 11619 12004 product=agamous-like MADS-box protein TM6 CDS 85338917 85339263 5056 5402 gene=LOC 115714657 CDS 85339360 85339426 5499 5565 gene=LOCl 15714657 CDS 85339517 85339578 5656 5717 gene=LOC 115714657 CDS 85339719 85339818 5858 5957 gene=LOCl 15714657 CDS 85344882 85344923 11021 11062 gene=LOC 115714657 CDS 85345082 85345126 11221 11265 gene=LOCl 15714657 CDS 85345480 85345731 11619 11870 gene=LOCl 15714657 >TM6 amino acid sequence from CBDRx (SEQ ID NO:11) MMSSYKSEPNNNIDQDTHHQYHYHHHHDHHEEEEEEDDHHDENSSNKQQKIGRGKTQIKFIENQTNRQVTFSKR RNGIFKKAGELSVLCDAKVSLIIVPNNNKLHEFITPGANTKEMIDLYQRTKGVDLWKSQYEAMQQTLRQLKAKN FELRTQIRQRLGYDLNDLSFHELSDLENSMLSSVEAIRHRKNHKIKTQAETTKKRNKSWEERNSVLMHEIHARC EDPPYGLVEDNVEGGGGGGYESSAVALANGASNLYAFRHHHNGGGSLITTSHSALHSGTGGGGSGGAYESFDLR DLRLA See APPENDIX C for the seven most common genotype sequences in Cannabis accessions for TM6. Gene name: STP6 CBDRx assembly gene ID: LOCI 15714521 Number of Isoforms: 1 Number of Indels: 7 Number of Exon SNPs: 101 Description of variation: Certain individuals contain insertions upstream and downstream of the STP6 start and stop locations, and there are two different insertions within the gene between exon two and exon three. The insertions are low complexity microsatellite-like areas, which can be difficult to align correctly. The CBDRx annotation is very similar to all other individuals. The present annotation for STP6 is slightly different from CBDRx, as the fourth exon starts 37 bp earlier. >STP6 nucleic acid sequence from CBDRx (SEQ ID NO:4) CCATTAATTTTTAAAGGAATAATTTTATTGTTATTAACTATAGAAAAGGATTAGTTAATTTATTAATTTAGGTT TTTTTTTTTT GAAAAAAAAGt T T T AC AT t AT T T T GAT T AAGAT T AAGAT T CAT GAT T T G C ACAT AT AGT T G ACAACATGGATTGAAATTAATTGGGCCTAGGTAGCAACAAAATTATTGGGTCATTTTAAAAAAAATTGTAGTAT TTTCAAAAATTACTATTAAAAGTGTGTATTTTTAAAATAATTTTTTGGAAGGAAATTTTCAAAAAATTTATCTT TTTATATTTTTTTTACAATTTTATGACCTAAAAATAATTGGGTCATTTAACGGTCACGTATCTATAAAAGTTCA AAAAATACAACTTAAGTATTATTTTCACAAAAAATTAAACTTAGGTGTTATTTACAATCGAAAGTTAAACTTAA GTATTATTACTGCCAAATTCAACTATAATCTCAATAGTTCAAGGAGACATTTTAAAGGTTAAAAAAATTCAAGA ACCCTAATTTAGTATATGTCAAAATTTAGTCCATAAATTGAATGAAATTGGATAAAAGTTGAATCTTAAACTCA ATGAAGGGGCCAGAAAATTAGTAAAATTGAATGAAGTCAACAGTTCAAGGAGAATTTTAACGGTCAGAAAATTT TAGGGATCATAATTTTGTATATATCAAAATTTATGGGGTAAAAGTCTCAATTAGGCTAGTCTTAATGATTCAAA TTATTAATATTGTTACATTAAGTTAAGAAAACAAGACACAAAAGCTGGAATAGCTCAGCTGGTTAGAGCGTGTG GCTGTTAACCACAAGGTCGGAGGTTCGAACCCTCCTTCTAGCGAATTTTCTTTTTCTAATATTTCATTAAGTTC CAT T T TAAT TAAT CAAAAT TAAT T T TAATAAAACTAATAAAAAAT TAT T T T GAAATAT TAAT TAAT CAT T T TAA AACTTATTTTTTTAGAAAATTTTAACTAAAATCATACATTATCGCCTTGACAAAACAGTGCATATCAGTCAGTA CATTATGCTTTTTTTTAATACCTAGAAATTTATTATCTTTCTCACAAAAATAGTGTTTTTTTTTTTTTTTTGTG AAAATTAGCGTTTATAGATTATAATAAAAGTTAGACCTCATGGTCTTTTCAAAGGATAGTTTCTGGTATAGAAG AGAGATCAAGAATGCAAGTAACATCTTTTTTTTCTAAATTTTTTTAACTAAAATCATACATTATCGCTTTGACG AAACAACCTGTCCATAACAGTCAGTACATTATGCTTTTTTTTTTTTAATACAGTACCTAGAAATTTATTATAAC TTTTTCACAAAATTATTTATTTTTTCTTCTTTTTTTTAATCAACAAATGTGAATATTAATTATTAATTAAATCT AATAAGTTTTTTTTTTTTGAAAAGATTAAAATAAAAGTTAGACCTCGTGGTCATTACAAATGATAGTTTCTGGC ATAGAAAAGAGAT CAAT CAT GT CAATAAC CTTTTTTTTTTCC TAATAAAT T T TAAC TAAAAT CATACAT TAT C G CTTTGACGAAACAACCTCTCCCTAACAATTGGTACATTATGCTTTTTTTTAATACTTAGAAATTTATAATAACT TTTTCTTCACAAAAATAACTTTTTTTTTTCTCATTAATTTTTATCAACAAACATGAATATTAATTATTAATTAA ATAATTTTTTTTTTTGAAAATTAGCGTTTATATATTAAAATAAATATTAGACCTCATGGTCATTACAAATGATA GT T T T T T TGGTATAAAAAAGAGAT CAACCAT GT CAGTAACAT T GT TAGT TAT GTACAT T TAACTAAGT TAT GGA CTGCAAAATTACAATCTCTAATAAGTTTTTTTTTTTTTTTTTTTGATTCAAGCGTTTATATATTACATCATGTT TTACCTGGGACTCGAACCCAGGACCTCCAACACTCACACACCCACCTATGGCCACTTGAGCTAGCCCCAAGTGG TTACAATCTCTAATAAGTTGATACTTGGTATATATGACTTGATTCGTCACTCTATTTCATTTTTTATTATCTTT ATTTATTATAAGATATGATTTCAATTATTTAATATGAAATAAGCTCTTATTTTACATATATTTTATATGTAAAA AAAT AC AC AT AT T T T AC C C GAT AAC C AAAT T T C AAAAC CAT AAAAAAT AC AT T T T T GAAAT T GT CT T C AAC AAA ACCTATCACGTTGTTATAAACAAAACCTATCAGTTATCTATTTTCATTAAAATATCGGAATAGGAATAGGAATA AGAATAAGAATAGACCCATATGATCCGATTTGGGGTTTTCCCTGTAAACCCACTTTCTTGAAGAAGAGGAAGAA AAGAAGGTGGGTGTGTTGTGGTAGTGAAACTTGAATTAGGGTTTTGAGAGGGAGAAGAATCGGGTATATACGAA AATGTATAAAAACTAGCTTCAAGAGCAATTCCACCAAATAAAAAATAGAATAGCAATTCCATTAGTTTGGGGAA TAGCATTACTATGCAATTCTTTTCCAAAATTGTTATTTTCCAACCAAACAAAATAATTAAATCAGGGCCATTAG TATTCAATTCCATTCCTTTCTGGCCCATTACCTCCAACCAAACGGGCCCTAAATAAATAATTAATATTCAATTT AAAGGATTTTTATTAAATAAAGATA7\ATATAATTAGTAACA7\ACACAGTAATTTTCTTCTTTTATTTTCATTTT TTTTACTAGAATCATTTATGGTAAGTATGATGGATTTCCTTTTAATTTTTATATAGTACTATATTTGGTTTTTT TTTTAGAAAAAACTAATGTGGGACTTGATTTTGTGTCTTTCATTTTTTGTAATGCATTTTTAAGTCATATACAT ACGACTAATTTGACACTATATATTGTGGTTTTTTTTAGTGTATCATAATAGTATTTTACAATCAATTAAATAAT TAATAGCGTAAAGGTCTAAGTTAGAACCCATAATTTTTTCTTTTTTCTCATCTCTCCCCTCACCATTAGTGGCT T TATATAT T GT GGTAT T TAAATAATAT CAACAGCAAT T CAATAAATAT T GT GT T TAT T TATATT GTT T CTAAAA TTTTGGGTTTTTTATCCTATATTAACCATATTAAAATTTAGGATAGAAAGTATTTTAGGCCAAAATATGAGAGA CACCTATCAAGTATACATTTATACTTAGTTTCTAATTTTTTTTTGTTTAACACTTCCTTAAATCTCAAATGAAG ATTGATATGATATCCTTATTTGGTAGCGTCATTCAAGATTGTAAAATGATACTTGCATCTCTTAATAATATTGT TGTGTCTTTTGTTAAGCGATCAGCTAACGTAGTGTTTCATAAATTTGTTAGAGCTGCTAGATTATATTCTGATC GTACTTTCAGTTTGGAGACTGTTCCAACTGAGTTGCTACCTTGTTTGGTAGCTGATGTGAGGGTATAATATCAA TAAAAGATGAAAT T T C CAT T CAAAAAAAAAAAAAAAT T CAAAT GAAGAAGAAAAAAAT TAATAAATAAT T T T CA TATTTAGATTTAATTTCGATTTATAATAATTTTGATCATTTTATGTCTTTTTAGATTTTTATAATTATCGCTAT T GAAGAAT T CAATAT T TATATATAT GACT CAGAT CACAAT GACATAT T TAT TAG C TAGAATATATAT T T T T GAA CAAAT GATAT GATATAAT GAT TAAT GT TAAAT T CAAT T T GAGATATAT T T G G GATATATAATAT TAT TAAT G GT GTTCTCTTTTTAAAATGGATCGAGCTTGGGATGAAAATAATAAGATATTATTAAGGGCCTTTTCAATGAGAGGG ATCATGTGTTGATTAAAAGTGTTTAACTGAGTAATAATGTTGAGGAAGATTGTTGGTTTTGGTGGAAGTTGTTG TTGCAATGATTTGTTGGTGTATTTAGAACGTTTGTACCGACTTTAGAGATACTAAGGTCGGCAAGAAATTTGGT AATTAAGTTGTGATAATTTAGTAAGCTTAATGTTGATGCTACTATATTCGAAGATGAGATTAAGTTTGGGGTCA GGTGTGTTGCTTGAGATCATGAGTTAAGGCTGGTGCAATGTGATTGTAGAGTTCGATAGTCTTGTGAGTGTTCA AG CAT TAT CTAGCACAGT T CATAT GGT CT CT CAAT T TAGT TTTTCGGTT GAAGAT T CT C GT T TATTAAT AT T C G AATTGTCTAATATTTCTTTACGTTTTGTTAAGTACTGTGCTAAGAGTCCTTATATTTTTATATATTTTTTTCTC GTATTGTATTGTGGTATGCAGTAGAAATTACAACATAATAATTTTCATTAAAATAATATATATAATTTAGTGAC TCCTTTTTGAAATTTTCGAACCCTCCTTCTAGTGAATTTTATTTTTTAATATTACATTACATTATCGCCTTGAG TTGACGAAACAGCCGATTCATATGCTTTTTTTTTATTAATACCTGGAAATTTATTATAATTTTTTCACAAAAAT AGTTTTTTTTTTTTTATCAACAAATATGAATATTAATTAAATCTAATAAGTTGGTAGTTAGCATATATATGACT TGAATCCTCACTCTGTTTCATTTTTCACTATCTTTCTCCGTATTCAATTTTGCCAATTTTATTATTTATTATTA TTTATTATAAGATATGATATTTCAATTATTTATTAAATATGAAATAAGCTCCAACCACAACTTTCTTATTCTAC ATATATTTTATATCTAAAAAATAGACACATATTTTTCACCCTATAACCGAATTTCATTAATAAAAACCTATGGA ATAAT CAT CAC CTAAAAAAT C GAATAAAT T TAAAC TAAACAGATAAT T TATATAC GAT T T T TAT TAAATAAG GA TAAATATAATTAATAACAGAATAGAGTAATTTTCTCTCCCAAATAACAGTATTTTCTCAGGATAAATATAGGCG TATAC CAAAT C CCAT C CT CAT C GTAT T CAT T CAT T CACAGT T TAGT TT CAT T T T T T T TT TTACT GAGT TAATAA TCGAAACCTTTCATTCATTTTTTTTCTTACTGTAAGTATGACGGGGGCAGTGATGGTTGAGTCCGGCGGTAGCA GCGGCGGTGACTTTCCCGCAAAACTTACGAAACAAGTGATCGTGTGCAGCATAATCGCTGCATGCGGCGGCCTG ATGTTCGGCTATGACATCGGCATTTCGGGTATTAAAATTATTAATATCATTAATTAAGTGGTCTGGTTATCCTG ATTGTATTCGAAATTTAAATATAATTTATTTTTGTTTGATTTATTAGGTGGGGTGACATCGATGGACGATTTTT TAATTAAATTTTTTCCAATCGTGTATGCTAAAAAGCACGAAGTGAAAGAAAATAACTATTGCAAATACGATAAT CAGTATTTGCAATTATTCACTTCGTCGCTTTATTTAGCGGCCATTTTGGCTTCTTTTATAGCTTCGATAACTTG TAAGGCTTTCGGGCGAAAGGTGACCATTCAAGCTGCGTCGGTGTTCTTCGTCACCGGTTCGGTTTTGAATGCGG CTGCCAAAGATCTCGGAATGTTGATTGCTGGAAGGATTTGTCTTGGAATCGGTGTTGGATTTGGCAATCAGGTA ATTTATTATACCATTTAATTTTTCATATCAATAAAGATATTCATTAATGTTGTTATATATTTTTATTTATTATA ATGATATTTTGTTATATTATATATGGTTAGGTCGGAAAAAAAAATCATATTTTTTTGGATGAGGATTGAAAAAA TATTTTTTTATAATTCATTAAATTTCAGGATCGGATCTTATATATAGTGAACCCGGTTGTTTATATTTATTTTT T AAAT T AAAAAT AAT T T T AT T TAT AT AAT AT GAT T T T T AAT TTATATTCTTC G GT AAAT AGAAT TAT AC AT T C A TAT TTTTGTGT GAACT AT AAT AT GAT ATT AC GT T T AAAAT T AAT GT GT T CAT ACT AAT T AAT CT GAACAAAT T T TAGAT CAT T GAT TAAT CAT GT T GT TATATATATATATAT GT T TAT T TAT TATAT C T TAT TATAAT TTTGTTATA TTTATAATAATTTTTATTGGGATATTTTGCAACTGAATAGTTAATCTGTAATTTGTTTATTTTCTTTTTTTTTT TAATTATTTTAAAAAAGGTAATTATATATATTAATTATATAGTGAATTCGTTTAATGTTGTTTTTATTTTTATT TATTTTTTAATTAAAAATATATACGTATATTATACTTTTCAATTTATATATATTCTTCAGTAAATGGAACTTAT TAATGTTGTTATATATTCATATTTTGTGTGAATTATAATATGATATTAGGCTTATACTCTTCATAATAATTTTT TTTTTATAATTTTTTTGGTTCAACATAATATGAGTCTTATTTTACATTTTTTTTATAATATTTTTCAGGCAGTT CCACTATTTATATCTGAAATTGCACCAACAAGATATCGAGGAGCTCTCAACATCTGTTTTCAATTATTAATTAC AGTTGGAATTTTGTTGGCCAATTTCGTAAATTATGGTACATCACGAATCCATCCATACGGATGGAGAATCTCAT TGGGAGGAGCTGGAGTCCCCGCTCTAATATTACTGGTGGGATCTTTACTGATTGTAGAGACCCCAACCAGTCTT ATCGAGCGAGGTCACATTGAGGAAGGTTTAAAAATTTTAAGAAAAATTCGAGGAGTGGAAGATGTTCAAAAAGA ATATGAAATGATCACACATGCCACTGAATTAGCTTCTCAAGAGAAGCATTCTTATAGAAAACTAATGCATCGTT CCAACAGACCTCAACTTTTGGTAACCGTGGCCCTGCAAATTTTTCAACAATTCACAGGAATTAACGTAATCATG TTTTATGCTCCTGTACTATTTCAAACCATCGGTTTCGGGAGTGATGCATCCCTTTTGTCATCGGCAGTCACAGG TAGTGTGAATGTAGCATCAACTTTTGTTGCAAATATTATTGTTGATAAGGCCGGAAGAAAGAGTTTACTGATTG AAGCAGCTGTTCAAATGTTCATTGCACAGGTAAATTCGATAACATTTATTTATTTTAATTTAATATATAATATG AAAAATATTAACTAAAATTATTTATATATTTGCAGTCTATAATGGGTGGTATACTCGCAAAATATCTCACAGCT ACAAGTACAATGCCGGATAAATACGCATGGATAGTATTAATCTTAATTTGTATTTTTGTATCTGGATTTGCATG GTCATGGGGTCCTCTTGGATGGTTGATTCCAAGTGAAATATTCCCATTGGAGGTTCGAAATGCAGGATTCTTCT TTGCGGTGTCCACGAACATGTTGTTTACTTTTCTTATCGCTCAAGCTTTCTTAACTATGTTGTGCCATATGCAA TCGGGAATATTCTTCTTTTTTGCCGCTTGGGTTCTCGTTATGTTCTTGTTTGCCATTTTTCTTCTGCCGGAAAC GAAAGGAATTCCAATTGATGAAATGGTTGACAGGGTATGGAAGAAGCATTGGTATTGGAAGAAATATTACAATG ATGAACAAGCGGCAACAAAATAATTTATTTAGTTTTTTTGTAGTTAACTTATTTAGTTTTTAGTTTGGTGTTTT TTCTGGATTGAATTTTGAATTTTGAATTTTTGGATTAATTTTGGAATTTCGGTTCAATATTTTAGGAAAACAAT TTTGGTTTAAATTTTAGTATTTTTTATGGGTTTCCTTCTAATTTTTATATAGTATTATATTTGTGTTTTTCTCA AAAAAAAACTAATGTGGGACTTGATTTTGGCACTATATATTGTGCTATTAAACTAATAGTAACAACAATTCAAT AAGTATTGTGTTTATTTATATTGTATATAAAATTTTGGGTTTATTTATCCTATATTAAAATTTAGGATAGAAAG TATTGTGCTATCGAGCATATGCATTTATACTTTAGTTTCTGTTTTTTTTTTATTTTTGTTCAACACTCCTTAAA T C T CAAAT GAAGAAACAATT AAT TAAT TT T CAAAT T TAGAT T TAAT TT C GAT T TATAATAAT TT T GAT CAT T T T ATGTTGTTTTAAATTTTTTTTATAGTTATTGCCATTAAAGAATCCAATATTTATATAATGTTAATTTGTATCAT TTGCTAGAAAAAATTGTTGGTTTTGGTAGAATGAAGTTGTTGCAATGATTTGTTGGTCTTTAGATTTTGCTCAA AAACACAAAAATAATTCTTTCCTAAGTTGTGATAATTTAGTAATTAGTTTAATTTAGTAATTTAGTAATTCTTT CAAGATAAGAGTAAATTTGGGGTTGAGTGTGTTGCTCAAGATCACATTGGGGTTGTTTTGGGTGTTATGTGGTT GAC C C GAT TAT GGTTGTTTT TAT T T T TAT T TAT T T T T TAAT TAAATATATATAC GTATATTATAGTT T T CAAT T TATATATATTCTTCAGTAAATGAAACTTATTAATGTTGTTATATATTCATATTGTGTGTGAATTATAATATGAT ATAAAGGCTTATTCTTCATAATAATTTTTTTTCGAATGTTATAATTTTTTTGGCCATCTTTCAACATAATATGA GTCTTATTTTACATTTTTTTTTATAATATTTTTTTCAGGTAGTTCTATTATTTATATCTGAAATTGCACCAACA AAATATCGAGGAACTCTCAACTTTTGTTTTTAATTATTAATTACAGTTGGAATATTGTCGGCCAATTTCGTAAA TTATGGTACATCACGAATCCATCCATACGGATGGAGAGAATCTCATTAGGAGTAGCTAGAGTCCCCGCTCTAAT ATTATTGGTGGGATCTTTACTAATTGTAGAGACTCCAACTAGACTTATCGAGCGAGGTCACATTGAAGAAGGTT TAAAAATTTTAAGAAAAATTCGAGGGTTGGAAGATGCTCACAAAGAATATGAAATGATCACACATGCCACTGAA TTAGCTTCTCAAGAGAAGCACTCTTACAAAAAACTTATGCATCGTTCCAACAGACCCCAACTTTTGGTAATCGT GACACTACAGATTTTTCAGCAATTTACCGGAATTAATGTGATTATATTTTATGCCCCTGTACTGTTTCAAACAC TCAGTTTTGGGAGTGATGCATCAGTAATGTGAATGTAGCGTCAACTTTTGTTGCAAATATTATTGTTGATAAGG CAGGAAGAAAGAGTTTACTGATTGAAGCAGCTGTTCAAATGTTCATTGCTCAAGTAAATTTGGTAACATTTATT TTATTTTAATTTAATATGTTTTGTGTGTTTCCTTCTAATTTTTATATAGTATTATATTTGTGTTTTTTTAAAAA AAAAAAAAAAAAACTAATGTGGGACTTTCGTGTCTTTCAGTTTATGAAATAATACATTTTTAAGTCACATATAC CACTATTTTGGCATTATATATTGTGATATTCAATTAATAGAACAACAATTCAATGAGTATTGTGTTTATTTATG TTGTATATACAATATAAAATTTTGGGTCTATTTATCCTATATTAAAATTTAGGATAGAAAGTATTTTAAGCCAA AATATGAGAGATACCTATCAAGTATACATTTATACTTTAGTCTCTATTTTTTTGTTTTTGTTTTTTTTTCTTTA ATACTTCCTTAAATCTCAAATGAAGAAGAAAAAATTAATAATATTTGGGATATATTTTGGATGATGATATTATT AAGGATCTTTTCAATGAGAGTTATTGTTCAATTGAGTAATAATGTTGAGGAAAATTGTTGGTTTTGGTGGAATG AAGTTGTTGCAAGGATTTGTTGGTCTTTAGACAATAGAATTTTGCTCAGAAACACAAAAATGATTCTTTACTAA GTTGTGATAATTTAATAATTAGTTTATTGTTGATGTTGCGATGTTTCAAAATGAGAATAAGTTTGGGGTCGGGT GTACTCAAGATCACATTGGGGTTGTTTTGGGTGTTATGGGGGATTGCATAGAAGGCTTAGTTGACCTAATTATG GCTGAGATAATGAGAATTAAGGAAGCGTTGACTTGGATCATTGGTCGTGGCTGGTGCAATGTGATTTTAGAGTT TGATAGTCTTGTGAGTGTTCAAGCAATATTAAGCACAATTTGTATGGTTTTTCAATTTGGTTTTTTGGTTGAAG ATTGTTGTTTATTGTTATTTGAGTTGTCTAATGTTTCTTTATATTTTGCTAAGCGCTCTGTTAATGGTGTTGCT TATTCTATTGTAAAAGCATCTCATTATCATTCAGGGTATAATTTGAGAGTGGATGATATCATTCAGAGTGTAAT TTGATTTAAAAAAAAAAAGTACACAAAATTATAGTATATGGTATATTTATTTATAAAGGTGGCAATTGTATATA AATTTCAATTGTTTTATTTTACATAATTACATAAAAAAAATTCTTATTATAGGAAAGTTGTTATGATAAAATTT CTATATATATATATATTATTTTCTAATATATACATTCTTAAAATATTTATATATATTATTGCAAAGTTTTAAGA GTCATTATATTTTTATATTTTTATTTCTCATTTTGTATAGTGGTTTGTAGTAGAAATTACGAAATAATAAATTT TAT T AAAAT AAT AT AT AT AAT T T AGT GACT CAT T T T T TAT T T AAT AAAT T T CAAT AT T CAAT TAT GT AT AT AAC AATATAAATTAAATTTATATATTTGTTATACAGACTATTACTGAAAATAAAAGAGGAACAATCGATATTACTGA ACAATCGATTTTTTAATTTTTATTAGGCCTGGGACAAAATTTTAAAGATGTTATTTTATTTAGAGTAATTTGTG GTATAAATGTGACAGTCAGTAGTCCCGTGATCCGTAAGGGGAACACCGGGTAAGCTGTGCAATCCCACACCGTT TGGGGATGGTCAAGTGGGATGATTCTGAGACTGTGTAGGTATGGGACTACACAGTTGAAGAGGGCTTAAATGGA TTGATTGGTACTACCTATATCAACAAGGTGCATCTTGTTTTAGTAGCCCATCTTGAAAGAACTCCACGTTAAGC GTGCTTGGCACGGAGCAATTTCAAGGATGGGTGACCTCTTGGGAAGTTTTCCCGGGATGCGTGAGTGAGGACAA AGCACGCCGAAAACACTCGTGTTGGTGCAGTAGGGTCGGTCATCAATCCGAAGCGACAGTGGCGTGCAGGTGTA TAAGAACCATTATTACGTGGGTGTAAGGACCTAATGGAGGCTTGAAGCGGGGCGTTACAAATGGTATCAAAGCC TTGACCCAGTAGGAAGTGTGGTCGACGAGGGCGCCGGACCCGTAAGGGGTGATTGTGATGATCGAGAGTCCAGT GATCCGTAAGGGGAAACAGGGTAGCTGTGCAATCCCCACCACTGGGATGGTCAAGTGGGATGATTCTGAGATTG TGTAGATATGAGACTACACAGTTGAAGAGGGCTTAAATGGATTGATTGGTACTACCTATATCAACAAGGTGCAT CTTGTTTTTCGGTAGCCCATCTTGAAAGAACTCCACAGTTAAGCGTGCTTGGCCTGGAGCAATTTCAAGGATGG GTGACTTCCTGGGAAGTTTTCCCAAGATGCCTGTGAGTGAGGACAAAGCACGTTGAAAACACTCGTGTTGATGC tcaggtcaggtcatcaatcagggaagtagagtgaagtggcgtggtgtaatataagagccattattctgtgggtg TAAGGACCTAATGGAGGCTTGAAGCGGGGACGTTACAATAAATATTTAATTTTAACTTTTAGTTGTAAGTAAAT ACTGAAGTTTAATTTTTAGCAATAATAAAATTAATACCTAAGTTATAATTCTGGAACTTATGTAAGACCTAACT ATAAAATGTTAAGCAAATTATCACGTGTCAATTCTTAATCGGTACAAGTGATTAAATTTTTATTTTTTAAAAAA AATAGTAAATATACCAATAAGAAAATGACACGTGGATAATGACTCGACATTTAACGGCCAGGTATCTACGGAAG T T C C AAAAAT AT AAT TTATGTATTATTTCCGC T AAAAAT T AAACT T AAGT AAT T AT T T AC AAT CAAAAAT T AAA 5 CTTAGTATTCTTACCGTCAAATTCAAATTCAGGAAGAATTTTTAAAGGTCAAAAAAATTTAGGAGTCATAATTT GGTACATGACAAGATTTAGTCTATAAACTAAATAAAATTAGACAAAAGTTGAATCTTAAACTGAATGAAGGGGC CAAAAATAAT TAT C GAAT TAGTAAAAT T GAAT GAAGT CAATAGCT CAGGGAGAAT T T GTAAT GACTAGAAAAGT TCAGGAATCATGATTTTGACAGGTCAAAAATCCTAAGTTGGCCAGTCTTAATGATTCAAATTATTAGTAGTTTT ATATTTTTTTAAAGAAAACTCAAAACAAGAAGCTGGAATAGCTCAGCTGGTTAGAGCGTGTGGCTGTTAACCAC 10 AAGGTCGGAGGTTCGAACCCTCCTTCTAGCGATTTTTCCTTCTTTTAATATTTCGTTAATTTCCATTTTAATCA ATCAAAATTAATTTTAATTAAACTAAAAATTAATTCTATATTAATAAGACTCTATTACATGCAAATATATTTGG ATGGAATTTTTAATATTTTTATTAGGAAAATTCTATAATGTATCCTTTTAAAAAGAGGGATACACTGATACATC TCTA Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 824664 829663 1 5000 upstream regulatory region gene 829664 832322 5001 7659 gene_biotype = protein coding 5kbs downstream 832323 837322 7660 12659 downstream regulatory region mRNA 829664 832322 5001 7659 Gene = LOCI 15714521 exon 829664 829872 5001 5209 product "‘sugar transport protein 8” (matches to STP6 in literature) exon 829966 830285 5303 5622 product “sugar transport protein 8" (matches to STP6 in literature) exon 831097 831723 6434 7060 product “sugar transport protein 8” (matches to STP6 in literature) exon 831804 832322 7141 7659 product “sugar transport protein 8" (matches to STP6 in literature) CDS 829734 829872 5071 5209 Gene = LOCI 15714521 CDS 829966 830285 5303 5622 Gene - LOCI 15714521 CDS 831097 831723 6434 7060 Gene = LOCI 15714521 CDS 831804 832235 7141 7572 Gene = LOCI 15714521 >STP6 amino acid sequence from CBDRx (SEQ ID NO:12) MTGAVMVESGGSSGGDFPAKLTKQVIVCSIIAACGGLMFGYDIGISGGVTSMDDFLIKFFPIVYAKKHEVKENN YCKYDNQYLQLFTSSLYLAAILASFIASITCKAFGRKVTIQAASVFFVTGSVLNAAAKDLGMLIAGRICLGIGV GFGNQAVPLFISEIAPTRYRGALNICFQLLITVGILLANFVNYGTSRIHPYGWRISLGGAGVPALILLVGSLLI VETPTSLIERGHIEEGLKILRKIRGVEDVQKEYEMITHATELASQEKHSYRKLMHRSNRPQLLVTVALQIFQQF TGINVIMFYAPVLFQTIGFGSDASLLSSAVTGSVNVASTFVANIIVDKAGRKSLLIEAAVQMFIAQSIMGGILA KYLTATSTMPDKYAWIVLILICIFVSGFAWSWGPLGWLIPSEIFPLEVRNAGFFFAVSTNMLFTFLIAQAFLTM LCHMQSGIFFFFAAWVLVMFLFAIFLLPETKGIPIDEMVDRWJKKHWYWKKYYNDEQAATK See APPENDIX D for the nine most common genotype sequences in Cannabis accessions for STP6. Gene name: DYT1 CBDRx assembly gene ID: LOCI 15704570 Number of Isoforms: GCF_029168945.1 2 Number of Indels: 0 Number of Exon SNPs: 34 Description of variation: the genomic region around the DYT1 gene contains insertions upstream and downstream of the gene but very few within the gene. The internal gene sequence is well conserved; there are only 34 SNPs. The CBDRx annotations are considerably shorter for this gene than all other individuals. Specifically, exon one in CBDRx starts -180 base pairs later and exon four is completely missing. >DYT1 nucleic acid sequence from CBDRx (SEQ ID NO:5) GATAAGATTATTTAATTTAACAGTTATTAACTTATTTACTCGCTATTGCTAAAAAAAAAAAAAGTTGCCTCCAA CATCCTTTCTATATGTAAGCATTAGCCCACTTATCCCAATGTATGATTAAGGTAAAAAAAATAGGATAAAATTA TTAGACCATCTTTATCTTCTAATATTTACTAAATTTAACGCCCCAAAAAAATAAACAATTGATAAATTTTTTAT AATAT T T GTTAT GGT GT T T C CAT GCATAATAATAT T T TAT GT T GCAAAT TAT GATAAGAAAAAAAATAT T AT T T CT GAT TAT GAATAAAT TAAATAT T TAAAAAATAACTAATAGTATAATAT T GTAACTAAAAAATATAT T T TAAGA CTTATCATAAAAATATTAAACTATATATAATTATTTTTGAAATAAAATCTTATAATTCTTCTCATAGTATGAAG AGCTAATATCTAAGCAAAGAGAGAGAAATAAACATCAACAATATTGAGAAGTAATATATTATTGGAAAAAAAAG ATAGAGAAGTGCTTTCTTCAAATAAATATTAATCTTTTAACAATAGCCAGTAATCAATTTTTTTTTTTTCAAGT ATAGT CT T T GT TAT T GACAAAT T T TATATAT GAAT T T TAC CAT TAT GTT TAAGT T T TATAAATAATATATAT T T GTTCACCTCTTATTTTTATATATAAACTCTTTTTACTTCTATTTTTTATATTATTAAGTTGTGTTTTTTATATT ATACATCTTGTAAATAATATTCTTCAAAGATTCAAAATATAACTCTTACTTTATAATATTTTATTTAATTTTAC AT GAAAT GAAT GAT GAAGT AAAT AAT AAGT AAT AAAT AGAT GT AAAT T AAAT AT TTTTTATGTTTTTTTATTTT AATAATTAAAAAATTAAGAATATAAATATAAATAATTATAAAAAAAAAGTCTTTTATTCTATTATTCACTTACT TAT T T T AC GAT AAAAT T AAAT AAAAT GT GAT GAACT ACAT AACT GT AAAAGT TAT AT T T CAAAAAAT AT TAT TA AGAAGT T GT AT AT T AT AAAAAAC AT T T T AAT AAAGT GAAAAAT T T GAAGAAC AAAAAT AT T AAT TAT T C C C T AA ATTATTATGTTCTTAATAATTAAAATATGCCTAAATCGAAAAACAGTGGCACATTTGTATGCTAGGTTAGTTGT TAGTAATTAATAATCTAAGACTAAGATAATTTTTGGCTCAGAGTTCTTTTGTGGCTGGCGGCGGGAGGTATTGG GTCTCCCCTGGCCGTAGGTGTTTGACATCTTAGTGGAGGGTTTCTTGCAGTTGAGAGTGGTGTCTCTCTCCCTT TGGTGTTTGGTTGTGACATACATTTTGAAATTTTTTTCCATCATCGATTTTGGAAAGTGGTATACCATTTGATT CGTGTTTGCTAATATTGAGCATTTCTTGAGATTGCGATTGAAAAGGGGAGGATGTTCGTGGTGCACCCTCATGT TCTTTGGTCTCTTGTATTGGTGGTTGTCGTTTGTTGGTGTGCTCTTTCTTGATCATTTTGTCTCGATATTGACT TTAGATGTTTGTAACCACTTGAGGCTAGCTCAAGTAGCTATAAGGAGGGTGTGTGTTCGAGATTTTGGATTCAA AT CCTAAATTATATAT GATT GTAATATATAAACGTT TAATTAAAAAAAAAATAGAT CATTTATAATATACTT GA TTTAGTCTTTATTTAGTCATTTTCTTGTGAAATTTGTGTAATTTTATTTTTACTTCGTTAGAATTTGTCCATTT AAATTGACGGATGAATTTGTCTTTGAATTTTTTTTTTTTTTTAATTAAACAGATATCCAATCATATTTAGTGTA GT T AACT ACACAAT GT AAT CAAT GT AT ACAAAGAT T TAAC AAAAT ATAT CT AT GAAT T T GAT AGT CAACAAT T T TTTTTTTTGCACCTATACATTATAAAAGCACCTAATTTTATTATTTTTATTTGTATAATATATTATTTTTTACC AT AT GAAGACAAGAAAAATAAAACATAAATATAT T T AT TAT T AGAATAAAAAT AAAAAT TT ATT ACT T T T AAT A AATAGATAAAAACAT TAT T GAAT T TAACACAAAACCATAC CAAATAAATATACATAAAAAGATATTAT GAATAA ATTTTATATTATTTGCTAGGTTTCACTTCCTAATACTGAAGTCGATGAGGTTATTCTTTTCTAAACTTGCTAGC TTTCTTTGGGTTTAGGCGTGGTTTGGTTGGAGTCTGAAATTTTACTAGTATGATTAGTCTTGTCTCTGTTATAG TTTTGAGTGCCTTTTGTTTAAGTTAGTGTGATTAGTCTTTTGTGATATAGTTTTGTTTTGTTTTATTTCGTTGT TGGTTCTTTTTTTGTTCTTCGATAGCTCACCATCAAATTGTTGTTAGTGGTTAAGAATAACAAGTTCTTTAAGC TGAATGAGTTGTTAGTGGTGTAGTTACGTACTTTGCACGAATGAATTTACTGCAACAGATCTATCATTGTGTCA GCGGTGGAGTAAATTGAATATAAGTGTTTTTAAAGTTAACTGTGATGTTGCAATCTTTTTCATCTAAATTTATT TTAATTTTATAAAACGATTCGGAAATCAGACAACTGATTAGTTAACTCATTTTTCTAATTCTAATTCTTTATTT AAGT T T CAAC GAGAGAGAT GT TAACAT T GAAT T GAAGAT T G C TAT TAATAAAAT T GTATAAT T T CT T TAAAAAA AAAATATATAAT CAAGTAGT C CAC CAAAGCAAAAGGTAC GC GTACAT GT CAAAC CAAACAACAT T CAAGT TACT CAAGAGTTTTACAAAGACATTGGCTTTCTTTATCCTTGTGTTCCAGTTCTAGTGCCGAAAGTATTGCATTTCTT CATAAATTGCTTTTAATTTCCAGATAGCTTAGTAATCGTTGTTTAATTATTTTTTAAGGAAAAAAAAAAGGCAA AATGAAGCACAGGTTGATGATTTTAGTGTCTTGTATAATACATTTGCTGTATTATTGTTTTATAGACAAAGTAA GGTGGCCTGTTTTCTCAAGCATAATCCATATCATATATATCCAGCATTACCTTCAAAACCAATTATTCCAAAAC CAAATCGGCAAACTTTTGGAATTATTATGCCAATGATTCCCTCCCGAAAAACCAGCTAAGCAAATTTATAAATT ATAGCCTCTTCTTCAGTCTTGACTCTTTCAATTCCACAGTGATAAAAGTAATTATATTTTATTGTAATATAATA ATAATAAGTTGGTAATAAAACTTTTCCTTTAAAATAGACAAGTTAAACAAAAGCTGTGGTTAATTTGTTTAAAA CTTGGCCAATAAAATAATTTACACTGTCCTCTCCTCTTGTTGAGGGGGTCCACTTTTCATCATAGTAACTTCTT AAAAGACGAAACAACCTTAAAATTAAAACCCTCTCTTCTTCTTTAATACCTCAAAAACTCATATAGCGGAGAAA CAAGGTTATCGTTCTTGTTTGTATCTCCAAGGAAGAAAAGAAAATGTCCGAAACACCCAAATCTTGTCTCACCC CGATCCTCCATCTTCTTCTTTTGATTCTAGTTTTTCATTCTCTGACGGCCACTTCTTCACTCCATAACCTGCTC CGAAGCCAGGGCTTGCCCGCTGGGCTCTTCCCGGACACCGTAAAATCATACGATCTGGACCACTTGGGTCGCCT AGAAGTGCACCTCGAATGGCCTTGCCTCTCAAAATTCGAGACTAGGGTATACTATGAAAGCGTCGTCAGAGCCA ACCTTACCTTCGGAGGGCTTCAAGGGCTTGAGGGTCTTTCTCAGGAGGAGCTATTTCTATGGCTGCCCGTCAAG GGCATATCTGTAAATGACCCTTCCTCTGGTCTAATTTTGTTCGATATTGGTGTTGCCCATAAGCGACTTTCTCT TTCTCTCTTTGAAGATCCTCCTGTCTGTAAACCTCAGGGTACTACTCTCTTCTTCTGATCTCTCTTATTATTAT TATTATTTTAAGGATTTTTAAATTTGAAAGTTTGGTTTTTTTTTTCTTCCTGGGTTTGTTCTTGGAAACTTCTC TTCAATAGAATCAATTCTAAAATTCAAGTTAAAGTTTTGTTTTTGTATTGCTAACAAAAAAAATCGAGGATATT TTAGATACTGCCAAAACAGGGGTTTTGGGTAAAAAGTAGTATTTGTTGGGCTCTTTTGCTATATAGTAGAATTA AAATATGAATTTTGGAATTGCAGGGATAAGGAAGAGTATGGGATTTCAAGTTCAGAGATGAAAAGGAGAGAACA TTTCATATCATGATATCCACCTTCAGATATTTTTTCCAGTGCTATTGTATGTTTTTGATTTTTGATTTCTTGAC AGGTACATATGTTTTTTCATGTTGATTGAAGCTATAAGGAGTTAAGGATAGTTAGAATATATGTAAATGTGTAT AAGGTTTGGACTTTGGAATCGAACGAAATTTGGAATTTTCTATTTTCTTAACATTGCTTTTTTTGGCTAACCTG AGAATCCAGTACTTTGATTTTATTGAAAAACCTCTTACATCTTGGGGAAAATCATATTTTCTGGGAGGATTCTA TACCAAATATGGGGGCTGAGATCACAAAATCAATCTGTACATGGTTTCATTAATTTGCTGGCTACGATGGATTT GGTAAAACTTCATTCATTTCTTCATTATTGTGAAATGGTATGGATCAAAATAGACTAATTGAGTTTAGATTCTT CAC CAAT T AAAT AAT AT T CT T T CT AT AAC C CAT T CAT TAT AAT TAT AT AT GT T T AAAT GT AT GAATT AGT GT C G ATTTTTCTTAATTGTTACCTCACAAAATATTATTTTAGATTATAACATTCCTCTAATGCTAAGAGCCCATGGCC ATGCCTAATCATTCTATCCAGTCTGTATTCTTGTATGTGCCTTAATTAACAACCTTAAGCAACAGTTCCTTAAT TTCTTCAACGGCAAGCAATTCACGAGAAAATCCCTGTAACCAATGAACTTAAATATAACTTTTTGGATAACTAA ACAATACAAAATTAAAGACAAAGGAGGGTTACACATTTGTTCTTGTGTTTTACCTGTAGACATAATGTAACTTG CATTACTCCTTTGAAGGTTGTTAAATTGGTATCAGTGATTTCGAATCCTAAAGCACTTAAGGCCTCTATCAGCT TAGTAAACCCACCTGGTTTCTTCTCAAAAATCACCTTCATCCAAAGTTTGTTTTCAAAGATTTGAGCCACAGAA ATATCTGGCTATAAACACAAACATCAAGTTAAAGAACGTTGCTGGCGTTAATGTTTGGAAATTACTATAGGTGA AACTTTTTTACTAAACCTGAAGCCAGAGCTCTTTATTCCGTTCTGCAGCACAAATATGTATGCCGTCTTTCTTT GGTACTGTTCCCTCCGCAGATGAAGTTTCTATACCCAAAAGCTGGTGCTGAAGAGTGGCCACAGTATGTTGCAG CTCGCTAATATACGCAATTGCATCGTCCACAATGGTTGCCTTCTTCATCTGTAACAAATCACAGACCAATGCTA TTAGTTTTACACTGCAAAAGTGATCTGTGAAATCACTGAAATGAGGGAAAATGTAGAAGGCAAAAAGTTTTCTC CTTTAGTTCGTTAAAATTACATTTGTTATTTTAGGGACTAAGCCACGCAACCGCAGCAGCTTGTCGTTGAGCTT CTCCCTCCTCCTCCTCTCAGCGTGGAGATTCTTGGACTTGAAATTGGATTCAGTCTGCTCGTTTTCGGGTTTCT TTCGACTAATCCTTCCATTTTCGCCAATGCACATTTCATCCAAACCAGAGCTCAGGATAAACTGGTCCATATCC TGCAAGTATTGGGAGCTCTTAGTTTCACCAAGTGAGGGAGGGAGAGAGCCCCTTTTGCGGCAGTATTTCGTTAA GTAGT GT T T CT GGGAGACAAGT TATAT GTATAAATATAT GTATATATATAT CTATAAATAT T TATAT T GAAAT T GGTCTTCACAAGGGTAACATGTTCTTTCGTGTGTGGAAGAGAGAGGTAATGAGATGCTACGGATATGATTATAA TACACAATTAGATAGGCACAATTCCTCATAGCCCAACTAATTTTGGCAAGAGAGAGAATAAGAGTTCCGTACGT GCGAATCAGTTAAGCTATTCCAAATTTTGTTATGGGTTATATACAAAACAAACAAAGTTTGGGGCCTAATATGC AAACCCCTCTACCCTCTAGTTAATCTAACACACCACACTCAATATTGGAATGTGTTTTAAAGCCATAAAGTCTC CATCTTTGATGTGTTTCAATAGCAAATTTTATATATATATATATATAAATATATTATATTTTAAAAGGCTAAAA AAAACGACGGGGGTTGTCTTGTGAGCATTGGTGGGAGATGGTCTTTACTCTTTGCTTTTCTGGTTTGGTGTTCA TGTCATGGTGGTTAGTCTTCTATTGACTTGAGAAGTACAAGAGAGTGAGGTTGTTGGAGCTGGCATATTCCTGA AAAGTACAATTCATTTGTGTAAAGCAATGCCAAAGTTGTGGTGTGTTAATCGAAAGGAGCTTTAACCGCAACCA GCATGGAGGATTGGGTTCATAATGAAGGATTAATCGTTGAGTTAAGAAGTATTATTAATTATTTTACATTTAAA T T GTAT GATATATATATATAT GTTTTTGTTTCTTTC TAAT T TATATACAAATAT T T TAT TAAAC CTAT T CAT TA AT T T GT T TATAT GTATATATAAAATGAT GT GAAGAAAGATAAATGATAGT T GAATAAT T TT T TT TATAAACAAA AT T TATATATAGT TAAAAAACAT T TATAAAAATAAAATATAGT T CAT C CAT GGT GT GTACT GT GTAT TACTAT T GATTTTTCGTGAATTAATATAGCTATAGTATAATTTATCTTTGCAATTAATTTTAATAAATACTTTTAAATTGT AATTTTTCTTTAAAAATAATGTCATATTTTGGACCATGTCTACTAATAAAGTTCAAGAATAACTACAAATTGGT GATAATTTATTTAAAAGAAAAATATTTCAAGGCTATAATTATTTTAATATAATTTTAATAGAGTTATACTGATA T C T CATAAAT T TAT TAAAATAT T T GCAAAT GTAT TAAT C CAT T TATATAT GT T T GAGAAAATAT T GT T TAAAAT TTTTAGATTATTATAGGAAAATTCTACAATGCACCCCTTTAAAATGGATACATTAATGTACCCTTATCTGTTTT AGCACCCGAAATAACTTTTTAGTTAAATTTTTTTATCATGGTCATATACGTTATAGTTATTTAAGACATCCTGC AAAATTTTAAAAAATTTAGAAAAGTTTAATACGCGGAAAATTATGTTCAAACAGTGTAGTGCACGCGTGACTAT TTTATTTTATACGCGTATAAAATAGATTGTTTGAACATTGTAATTTATATTGTAAATTATTCAGAATTTCTCAA AATTTTGTAGGTTATCTTAAATAGTTATAACGTAAGTGAATATGAAAAAAAAATTAGACTAAAAAAATTTTCTC GATACCGAAACAAGTCAAGGGTGTATCAGTACATCCCTTTTAAGGGGGTGCATTCTAGAATCACCCATTATTAT ATATGTCTAATTTTAAATTCAGATATTTTTCTTTTTATTATTTTACACTCAATTATCACATTCATTTTGTTTTA ATTTAGTTTTATGAATACTAAATTACTTTAGACTGAGCGAAGCGTAGTTTCATCAATAAACCCCAGCCCAAACC CCAAAACCCCTAAAGTCCCATAGAAGAGCAATGTCGTTCTCTCAACTCCTTCAGCGCGCCTTCTCAACCACGAC CCAGGCGAGTTGCGCCGCCGCCACCAACAACTCCCTCTTCACTGTCGTCTACAAGGAGCGTAACCTCAAACGCC TCGTCGAGAAGTTCAAGAAGTCCTCCGAGGCTTACCGTTTCCGAACCAAATCTGGGATTTATGAAGACACGGTT CGCCGCCTTGCCTCTGCTAAACGCTTCAAATGGATTGAAGAGATCCTCGAAGACCAGAAGAAGTACAGCGATAT CTCCAAAGAAGGTTTTGCGGTCCGGCTCATTTCTCTCTATGGGAAATCGGGGATGTTCGACAATGCCCAGAAGG TGTTCGATGAAATGCCTGAGAGGAACTGCGAGCGTACATTGTTGTCGTTTAATGCTCTTTTAGCGGCTTGTGTA AACTCCAGGAAGTTCGATAAGGTTGATGGGGTTTTTAGAGACCTGCCCGAGAAGTTGGCGATTGAACCAGATTT GGTATCCTACAACACCGTTATCAAGGCGTTTTGCGGGATGGATTCTTTGGATTCGGCTGTTTCTGTACTGGATG AGATGAAAACGAAGGGTCTGAAGCCGGATGTGATTTCGTTTAATACATTATTGAATGCATTTTATGGAAAAGGT CGATTTGCTGATGGTGAAAGGATATGGGATCTAATGGAGAAGAATTATGTCTCCCCTGACATTAGGAGCTATAA TGCCAAGCTGCTCGGATTGTTTAAGGACAAGAAAACAAAAGAAGTTGTTGACTTGATCGGTGAAATGAGGGCTA AGGGAATTGAACCAGATGTGTTTAGCTTCAATCTGGTGATTGAAAGTTTTGTCAAAAGTGAGAACTTGGAGGAA GCTAAGCAGTGGTACAATGAAATGAAGAAGTGCGAGTGTGTCCCAAATAAAACTACCTTTGAAAAACTCATTCC CTTCCTTAGGAAGCAGGGCGATTTGGAGTTTGCTTTTGAGCTTTGCGAGGAAGTTTTCAAAAGGCGATTCCTTT TAGATGTGGCATTATTGCAGCGGGTGGTGGAAGAATTGGCTAAGGCGTCCAAGATTGAGGAAGCAAAGAAACTA GTGGAGCTTGGAAAGACTAACGATTACCGTCGCTATCGCCTGAAGTTGCCTGAATTGCCTTCAGAATCAGAGGT TCCTTCAGACAAGTAGCGCCTGCCTTTACTTTTATTATCTTGCTTTGCTTGCTAATTGTCTTTTTGTTCTACAA GAATATTTTGAAGAGACAGACGATTGTCTTTTCCTAGTTGGAAGTTTAAATTATTTCTTAGCTCTTGCCTCAAA CTAGACCGCTGGCCCACTAGACAAGTCAAGTAGTACACTCCAGTTGTCAATTTTGGTTGGTCACTCCTTAATAA TTTATGAGAATTGTATGCTACTTTGTGTTGCTGATATACTATATTTCTTGTTTGCATAGAACCAACTTCTCTGC TTGTGTATTTATAAGTAGCGCAAAGTTAACTATTTTTTAGGTGTCACTGGTTTAAGCTTTAAGCTTTGAAGTTT TCTGCTTCTCAGGAAAACTGTGAAGAAACTTCAGTAACCAGAATCTATTACTCTCTTTATAGTAAGACTACTTC TGGCATATTTTGTGGGTTTAGCAGTGAAGTAGTGTACTTGTGAGGTGTACACTTCATTTCAGCCTGCTTACCCT GCTGCATTAGCTTGGCTAGCTTGTGAGTTGGCTCAGCATTTCCATTTGTACATGAATTGAAATTCAACTTTGTT ACTCATTGCGAGTAGAATTGATTCACGTCATGATCCATATGGTTATTTGATGTCGAACTTGAAGGTTTGGCGAC AGAAGAAAGTGTATTATCAAATTTTGGGACCAAGTTAGTAAATCACAACCATTCTATCAAGTTTATTAGGATTT T T C GT C CACAT T GT T GAT GT T GT T GGATAGAGTAACAC CAC GCAACAGAGATACAGGAACT CTTAATAT GAGAC TTACCACTGATTTTCACTTGACCAATTCAGATGGCAAAAATGGCCTAGCTTTTCAACTCGAAGTTATCTACATT CTGTTTTTGATTGATTTTCTAAAATTGATATTTATAGATATTTCTTTTTTAAGTAACTATCCATGGATATTTGA GAAATGTTTTAGAACTGAAAAAAAACATACAAAACAGAGAAGGAAAATTAACAAATAAAAACTTATCTGGCTTC ACATACATTTGTTTATTTTTGTTAAAGTTGGTAGACCTGACATCATAGCAAGATTTTTTTTTTCTTCATATTTT ATAGTTATAGAAACCACATTTAGGCGTACAATAAAATATCTTATAAACTACAAATATACATTAAAATAATATTA AAGTTCTCTCTATTTGTTCCAATAAATCCTTTTTATGAAAGAAGTATGCATTCACTAAAATAAAGTGAAAAAAA TAATTACATGAGCATGAGCTTCACCATGTCATCAAACTCATCACTCATGAGGGACTGTAATGGTGTAAAAAGCC CAAACTAGGCTTAGGTTTAGCTGACCTGTTGGGCTTGGGGAGCCTTCATTTGATGCCTGTTATAATAAAGGTTT ACACAGAAAAACAAGTATT GTAAGCGAGTGAGT GT CT GCAAACAAAGACACATAAAAATGT CTT CTCT GT GT CG TCGTGTTCGAGCTATCTTTACGAGAGTTCCTTCAAAACCCAGCGCCAACTTCTGCACCACTGAAACCCTTGGCA ATAAACGATTGGTAAA Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 93242539 93247538 1 5000 upstream regulatory region gene 93247539 93248137 5001 5599 genebiotype = protein coding 5kbs downstream 93248138 93253137 5600 10599 downstream regulatory region mRNA 93247539 93248137 5001 5599 gene=LOCl 15704570 exon 93247958 93248137 5420 5599 product=transcription factor DYT1 exon 93247665 93247875 5127 5337 product=transcription factor DYT1 exon 93247539 93247618 5001 5080 product=transcription factor DYT1 CDS 93247958 93248137 5420 5599 gene-LOCI 15704570 CDS 93247665 93247875 5127 5337 gene=LOCl 15704570 CDS 93247539 93247618 5001 5080 gcnc=LOCl 15704570 >DYT1 isoform XI amino acid sequence from CBDRx (SEQ ID NO :13) MDQFILSSGLDEMCIGENGRISRKKPENEQTESNFKSKNLHAERRRREKLNDKLLRLRGLVPKITNMKKATIVDD AIAYISELQHTVATLQHQLLGIETSSAEGTVPKKDGIHIRAAERNKELWLQPDITVAQIFENKLWIKVIFEKKPG GFTKLIEALSALGFEITDTNLTTFKGVMQVTLCLQGFSRELLAVEEIKELLLKWN >DYT1 isoform X2 amino acid sequence from CBDRx (SEQ ID NO :14) MDQFILSSGLDEMCIGENGRISRKKPENEQTESNFKSKNLHAERRRREKLNDKLLRLRGLVPKITNMKKATIVD DAIAYISELQHTVATLQHQLLGIETSSAEGTVPKKDGIHIRAAERNKELWLQVIFEKKPGGFTKLIEALSALGF EITDTNLTTFKGVMQVTLCLQGFSRELLAVEEIKELLLKWN See APPENDIX E for the seven most common genotype sequences in Cannabis accessions for DYT1. Gene name: MYB35-15 CBDRx assembly gene ID: LOCI 15700367 Number of Isoforms: 1 Number of Indels: 20 Number of Exon SNPs: 120 Description of variation: The genomic region contains an insertion in the upstream region. The CBDRx annotation is significantly different from other assemblies in that the starting position of exon one is ~285 later. There is an additional fourth exon in the CBDRx assembly, and the third exon in other assemblies is split into two separate exons in CBDRx. The last exon in CBDRx ends approximately -250 bp earlier compared to other assemblies. >MYB35-15 nucleic acid sequence (SEQ ID NO:6) TGTTCAAAACAAAAGAATATATTACAATACTATATATTGAATATTCCAACCAAAAAAGAAAAGATAAAGCTTGA AGATAAATCTACCTACTAACAGTAACTTATCATTTTCATATAATAATCATTTCTTTATTAAAGAATAATAGTCT GTGTTGTCCTCATTCACTTGTACAAGTACAACACACTAATAACGTATGGAGTATATAGACAATTGATAAAAGGC ACTTTGGTTCTTAATATTCTTTTCCTTGTCTTATTATTATGAGAATAATTAAGTATCAGGTCTTATTTAATTTT AAAAAATAATTTTTAAAAAATATTGTTAACATACTCGACACTAATGATTATAACAATAACATTTATTATTTATA TAAAAGAAAAGACAAAAGTAATCAAAATT T GAAT TTGTTCCTTCCAAC CAGAAAT GAATAT GCAAAGAT GT TAA AAAGGGAATGCAGAGAGAATTTTCAAACAAGCAAATCAATATTATAAAGTTTAAAAATAAAATTCTATAATAAT CATAGTGCAGAAAAAAAAATTATATGTATTAAATATATTATTCTTTAGCTTATCCTGTAAATCATATGTATTTT TATATATACAAATTGGTACTTAAATATATATGTAAAAACTAAAAAGTGAGTAGATGAATGAATAATGAATATCT T G G GAAAACAATAT T TATAGTAAAAATATATTATAT TAAGAAAAAAAAAGTGT TAGAAGT GAGAGTT T T C C C GA CAAAT T CAGT CAT TAAAAGAAAACAAAACACAC T GAGAT T GAGAGT GACAGT TAC T TAGAGAAAGAT G C C T T T G AGGGCCTACTTATTAATTTTCAGTATCTTAATTAATTAATTACTACAATTTTATTAATGTAATTGAAAAATATA ATACATATACGTAAGCTTAACTTAAGTTTGAAGTCAGTAACTCAGTAACTGAACAAAAGCTCTGCTTATTGCAG TGAATAATAATATATATATATATTTAAGAGATGCTAATCTTATTTTGGATTAATTTTTATATCATTAATATCAA T CAGT CT T GTAGTAAGTAC CAT CAT TAT CAGATAAT GT TAC CT GCT GT CACAAACAGT CAGT TT CT GT T TAAGA TTTGCTAGACCAAATTTCCACATATCAGAGAGTCTCACTGTAAAAGAAACCCCTTCAATAAATAAATCATGTTT TTTTAACTAACTTTTATATTATCTTATATATTATGGGAAAAGATTATTTTAGATCCACTATTTACAAAAGTTAT TAGTTAGATCTTCTTTTGTTAAATGAAAATGGATCTTGTATTTTTTAAAATTATACAAATAGAACCATGAACTG ATT T T T TTAT TAAGAT GT TAT GACAAAAGT GGTTATAT T TACT GTATT TAT T CAT GT TAAAAATT GT CT TAAAA T T GAGTATAT TAAAAAAATAAAAATAAAATTAT T GAAAAT T GAACT CAGAGT CATAT T T TAACAATT T TAAAAA AT AT AAAAT AT TT TAT TAT T T ACAAAAAT AAAAGGT AACT T T TAT T AAACACAAGT TAT AAAAT AGT AT T TAT C CATATAAATATAAAT GAAAGAAAACAATAATAAAAAATATATT TAGTTAAGAGGTAAAC CT TAT CTAAAT GGAG ATGTAATATTTATTTATGAGCTAAAATTTTAAGATACTCATCTATTGGAAGGGTCATTTGAAGAGTATTGAGGT ATAATTTAGTTTTGTAGCTATTATACTTTATTTATTGGGAGTACTCTTAGAGCAGTCAATAACTAATGTAAAAT AGTTAGAAAGGTTAAAAAATAGTTAAAAGTTAAAAAAAAAATGTCAAAAATGTGCATCTAATAAATGAGCTAAA ATAACTTAAAAATTTACCTTATGAATAATGCTTAGCTAAATGTAACTAAATGTTATCTTAGTTTTAGTACTTAG TTATAACTTTTTTATTATTATAATGTATTTTTTTAATTATAAGTGTAAAAATAAATAAATATCATATAATATAC AAT ACT T TAT TAT T T GT GT GT AT TAT AAAT AT AGT AT T T AAAT GAT TT AT AAAAAAT TAT AT AAT AT AAAT GT G T GAAGTAAAATAAAATAAAAAT TAAATAATAGAGTAAAAAAACT GT TAAGAGT T CT CAC CATACAAAAAGATAA AATCTTTAATAAAATAGCATTTTTTTTTTATTTATACACTAATATCTCCTTCAAATCGTCTTTGTCACAATAAA AGTTATTTGTGACCACTTAAAATTCTTCTTCTTTTTTTTTTTTTGGCTATATTATTCATTAAAATATAAAGTAA AATTTATTTGAAAAAGTGAGATTATTTAATGCCACTTTGCTTGAATTGCTTCATATCTCATTCTTTTTATTGAA T G GAAAAGAAAAAGAAAT T GT AT T T AT AT AT T T C T T AAAGT G GAAAGAT AT AAT AT AAT AT AAT AT GGT GAGAA TCTATAGAATATTTATTAACAAACTATAAGATATAGTATTCTTCTAGCTATTTCAATCTTTCTCTCTTGTGAAT TAAGAAAGCCCAAGTTTAAAATAGCTAGCCCAGCAAAGTAGTACAATGCCCAAAAGCTTCAATATTCTTTTCTC CCTTTACTTGTTTTTACTTTTCCCTAAAAAATTTGTACTAAGAAAAGTTGGAGAATTAAAAGAGAATAAAACAT ATAT GTATATAAGT GTAGTAGGATAT GGAGT GAC GC CTAT T C GAAT GT GTAGAAAAAAAAAAAAGAAATT TACA TGGTATACTAACTTTTACCATTTTTTTACAAAAATCTTGCTGCAGAAGTATTTTTTTACTTTCTTGTATTTTTT TATAAGTTTCATCTCTAAGAGTATCTTGTGTTAAGTTTTCACTGGTATTCTACTGGTGTTTTACTGGTATTCTA TTGTTGTTTTGAGCTGTTCTGCTTTGTGACAGTATTTTTGTAAAAGTTAACCCAAATTCCAGTATTTTTGTAAG TT T CAAAAAAAAAAAAAAAAAAGTAAAAGAGAT CAACTTTAGAGCATATACAT GT GGATAAGAGGAAAAAGGAA AATATTTCAAAACACTTTAGGGTTTGAGAAATTTAATGGCAATGTAACATTGTTTTGTGGTGGTGCTAAATTTT ACAAGGCCTCTAATTCAAAAGAAAAAAACGAACATTTTATTAATTATATATTAAATTTTAATAAATACATATAA ATATAGGTGTCGTCTGGTAACATTTTTTTTAATCAGTTTTGTGTTTTTAAAATTGAAAAAGTGAAAATATTTTT CAAAAACATATTTTATAAAACTGTTTTTACTTTTTCATTTTTTTAATTGAGAATCAAAATTTTAAAAACAAAAA AAAAATACTTTCAATATTTTTTTTAAATAGTTTTTTTTCTTAATCAATATCTTGGACTTCGATCATGCAGGACC CAAATACCCCACCCCTCCCCCGACCACACTGGAATTTGAACCAGACCTTTGACTTGAACTTTGAATCAGGCCAG TTTTAGACCC GAT GTAAATAAAAT TAAAAAAATAAAAAT GAAAATAAAC T T TACAGAACACACT TTTGTTTTAT GTTTTTAAAATTGAAAAACAAAAGTGGTTACAAAACGCATTTTTGTTTTTCAAAAACAAAAATTTTACTTTCAT TTTCTGATTAAAAATTTAGAAAACATAAGTGTTACAAAACGGCACCTATAGTGTTTATATATAATGTCACATTT AGTTACAGTTTCACTTTTTAAAAATAGATAAATACTATTTTGGACCCTGTGTTTTGCAAAAATTACTAATTTGA CTATCTGTTTTGTTAAATGACAAAATAAACCCTGTACTTTCCAAAATGGTACAAATAGGACCTTGAACTAATTT TTTGTCAAAATAAAATTTAATAATAATCCGATCTAAATGTGTTATGACGAAACTGTTTACATTTTCTGTATCTG TTCGTATTAAGAATTGTCTTCAAGTTGGTTATATTAAAAAAAAAGTTGTCGAAAATTAAGCTCAGGGTTCTATT TTTACCATTTTCGAATATACAGGGTCCAGATAATCATTTAACAAAATAGAAAGTCTAATTTATAACTTTTACAA AACACGGGGTCCAAAATAGTATTTATCCTTTAAAAATATATTTTTGAATAACTTATAAACACAATTGTTTCTTA ATAAAAATAAAC GTAAATATT T TAT T T TAAACT TAC T TATAAATT T TAT T GAAAT TAT GT GATAAAAACTATAT T T AAT AT AT AT T T AT AAAAC AT AAAT AAAAT T T AT GAAT AT GT T T GT AT GT AT AT AAAT TAT T AAT T T GAT AT G AAAT TAT AAT AT AT AT AT AT AT TAT T T T AGTAAT AT AT CAAAAT AT AAAT AT T AAT T T AT AT TT TTAT AGT T AA TTATGAGTTTTTTAAG GAAAT GAAT TAAAAATAAT TAATAC GT TAAAAT T GTAAATAT T TATAT T TAT T T T TAT TATATAAT GTAAATAT GAAAAAT TAT T TATAAATAT T TACAAAATT GTATAAAAT T GT GTAATTATT TAT T T TA TTACATTTTTTGTAACATCGTTAATTTATTAAAAGTTAATTAATTAATAAAGCCGCATAGGGAAGAAAAGAAAA CTCAGTGCATAATCATATATAATATAGCTGTAAATTAATTTGCAAAGAAGATCATGCACTCACTCACCTCAATA ACAATGCTTTTGGGGCCAAGCATGAGAAAGAGTGAGAGAGAGAAGAGATCACTATTTTTGTGATGTCATGACAA TAAACTGTTTATTATTATTTTCTTCTAATATTATGAAGAAAATAATCATGCAATTGTAGTTATTATAACCCCAA AATTACATGCATTTCTTGCCTTGATATTCATATCCCTTTTCTCTTTCTTTCTAAAGATTCATTCTCTCATTTTT TTTTATAATGTACATGCAAGAGCATTGGAGTTTGCATCACCCAATACCATAATCAATCTTTTATAATTTAATTT TTATACCAACTTTCTCTTCTGATCTTCACTACTTACACAAAATGGGTAGACCTCCTTGTTGTGATAAATCAAAT GTGAAGAGGGGTCTCTGGACTGCTGAAGAAGATGCTAAACTACTTGCACATGTTTCTAAACTTGGCATTGGAAA TTGGACTTTGGTCCCCAAAAAAGCAGGTCTTATTACATATTATATATACTTTACCATATTAATATTTTGATGAA TTCTACATTTTTTTCTTTAATTTTGTATGAATTTTCATACACAATTACAGGTCTCAATAGATGTGGGAAAAGTT GTAGATTGAGATGGACAAATTACCTTAGGCCTGATCTTAAACATGACACTTTCACTCCTCAAGAAGAAGATCTT ATTATTAATCTTCACCAAGCTATAGGAAGCAGGTAATTAATTTTATTTTATTAATTTTATGGATTTTTTTTTTT ATAAAAGATTAGAATTAAATTGATTTATATTTATATATGTAAAGGTGGTCTCTTATAGCAAAACAGCTTCCTGG AAGAACAGATAATGATGTGAAAAACTATTGGAATACAAAGCTTAGAAAGAAGCTGTACACAATGGGAATTGACC CTGTAACTCACAAACCAATTTCTCAAATTCTTTCTGAATTTGGAAACATCAGTGGTCTTCCTAATAATCATCAT ATCCCATCCACAAATATGATCAGTAGTACTACTACTACTACAACTCCTGCTAGAATTTTCTACAATAATAACAA TAACAATATTGGAACAAGCCATCAATTTCAAGTAATGACTAACCAAAACCTCTTTAACAATAATAATAATAGTA ATGTTCAACCACACTTTTTCAATGAAGTAACTTCCCCTTGTTCTTCATCATCTTCTTCTCATGTCACTGACTTA AGCTCACCACAAGTAGTTCTTTTCGCCCCGCCGGCTCTGCCTCCGCCTCTGCCTCTGCCTCCTTGTAATTCCAA TAATTGGAGTGAGTTTTTTCTTGGTGACAACACTGAAGATCAAGCAAAGGAGAATGATTTGGAGGGTTTTTTAT TGTCATCATCATCATCATCACATACTAACTCTTCAAAGTTAAGTAATGGAAATGGTAGTGATCAAAATGGTTCA AACATTCAAGAGGGTCAATTTGCTCAGATGATCAACAATGATCATCATCATCATCATCTTCAACCCGAAAATTC GTCATTTGTGGATGCAATTTTGGATCGAGACAGCGAATTGCGAGCCGCCTTCCCCGAGTTCTTGGATGGATCTT TTGAATATTAGTTTCCATACTCGTTGGAATTGACAACAAAATGTTTACAATAATACTTTCTTCTCTTCTTCTTC TTTGTGTTTTTTTCCCTACAATTTGGTGAGGGAAATAATAGGACTTTTATATTGCAAGTCATAAAAAAAATTAT TGTGGTTGTTTTGGTTTACAAAACTTTTTATGGCTTTGATAAAAGCTTGTATTTTTAGTTTATTAATAATGCTA TTTGTGAGGTACTTTAGCTATTATATAACTGTTTCTTTTTTCTAATGAAACATATTTTAATTTGTGGACAATTA AATAACAAGAAATATGGTTAGCTGTGGGAGGCAGTGTGGTTTAGCAAAAATATATTTTTTTGGTTAAACGATGA GTTATCTTACATTTATTGTGGATATTTGTGTCTAATTATGTTTAAAAAAATACAAAAAATATATGGTAAGCTGC TAGTATGGACTAAATTAATAAAGAAAAAAAATGTAACTTGCTTACTTCCAACAAATTAAGACAGATAATCTTAT AT T CTAATATATAAGAAGT CCTAATAATAGAAAT GAAAT CACAAAATGAT GAAAAAGAGTAGTTAATTAT TAT C TAAT GGT TAG GTAATTAAT T GAG GT TAACAATAAAGT T TATATAGT GGAGAGAGAGAAT GAATTAAT TAT GTAT CATATATAAAAACTGAATCTACACAATTCTCATCCACTAATTAATAATTTATATTGGTTTACAAATTATATATG TGTATATATATAGTACTATATATACTCTCTCTCTCCTCTTCATCATCATCATATCATCATCATCAACAAACAAA CTCTCACAAACTGTGAAAAGAGAGAAAATAATCAAAACAATAAAAACCGTACGTTAGTACGTTCTTGTTAATTT GT T GAAAATAAAGAAGAAAAAAAAAGTAT TATATATAT GAGAT T GATATATATATATATAAT T GTTAT T GAATA ATAATAATAATAATTAATTAATTATAATGACTTCGTTACAAAGATCTGTGGTGTCTTTCAGAAGACAAGGGTCA TCGGGTCGTATATGGGATAATCATCTTCAAATTCATGATCAAGGGCCTCATCATCATCATAATAATATTAATAA TAAGGTATCTTGTTCTACCTTGAATCCCAAGAAAACAGAAGGTCCAAATAATAATAATAATAATGTAGAACACA GATCAAATATTATTAATAATAATAATACATCTCCTCAGAATAATATTGATCAGAGGGAACAAAGATCATGTGCT TTATGTGCCTTGTTGGGACGATGTATGGGATCATGATCATCATCATCAATTAATTAATAATCTTCTACCAGTAC TTAATATTAATTCAAAACCTCCTTCCTATGTATATTAATGCCCTTCATCATCTATATCTTAATATTACATTTGT ATATATAATTTCATCATATTTTTTCAGATTATGTTGTAATTAATTAACATCATTATTATTGTCACCCTTTATTG GTATTTTCACGAATATCATAATTCCCATCCTCTTTCATTGTGTTCATTATTGTTTTTTTTTTTGCTAGATTACA CGAAATTAATTGAGGTTACGTTTTCGTACGAATTCAAGATGTAGATTAAGTAGTTCTTACCTTTTTTGTTATTA ATTAATTAATTAATTCCTTTAATTTGCTTAATTTTGAATATGCACTCAAATTAATTAACTTATTAACAGTTTGA ATCTTAACGATCCAATAAAAGAATGAAAATAAATGCAGCTAGAAAACAATAATAATAATTATAGTAATTAATTA AGGTTAATTTGATCAACCATTACTTAATGACCGCATGCAATCATAAAATTACTCTCTCATGAGGCCCTCTCTTT GAACTTGTGTGTTGTGCTATCGAAAACTCAAAAATCTTCTTCCCAATCACAAGTGCTTGTTATATATATATATT TGTAAAACTCTTAATCAGATTGGTTAAATATATGTATAGCGTGTATGTATTTCTATACGTTGATGTAAACAAAA TTACATTTTCACATAGTGGAAGAGTACTGAAATTTGAATTTTCATTGTACGCATCTTTTTTCTTTTTTTGAACA AGTCTTAATTAAAGTGATACATTGATAAGTCCAAACATCCAAAAAATAAGTTGATTTTGAATAAGTTGACTAGG TGTGGTACCACATGTTGTCCCATTTAGTCCTGTTGAAAATGTAAAAGAAGACATGTTGCATGTTTCTTGTTGCT AAACAT GTAGAGGATAATAT T GT CAT CTT TATATAAAATAGAGGAATATAT T T T GATAT TT T GT TAAAT T C C C C TCACAATTTTACAATTCAAAACAAAATAATGCGGAATTAGAATGAAATATCAAACACAGAAGTTTTTAGTAAGG TTCATCACTCTAATTTTTGGGAGCTATCCTCTGAAAGTTTAGCTCTTTCCACTATATCCAAACTTGAATAATTA CAAAGGTGTTGAGATTTTATATCACATTCTTTGACAAATAATCTCTATAAACTCTCTCTATTCAAATTGTTTTT CCTCATTCTCTTGCAGAGAACACAAAAAATAACTCTCTTTCTTTCCTCTCTATCTCTCTAACACTCTTGAGCTT CTCTCTATAACTTGAGGAGAATTCTTGCTCTACTGAAGAGTACCATTTCTCTTCTTTATGTATTGTTCTTCGTG TTCATGAGAGTATATCTAGGTGCTGAAGATGAGATTCTTAAGTTGATAGTTTGTAATTGATTTCAGTTAAGTTT GTTATGCCTTAACAAACTCCTACAATTAAGATCTCCACGTTAGCACTTACAAAGTTACTCTCCGAATGTGGAAC ACATTCCTGAACTAAACGAGTCGTGTTATTAATTAGAAGGATGAGAAAATGCTTTGAAGCTTCTATCGGGGAAC AGCTAGCCTTCCAAGACTCTCTTTCTTGAAGGATGACTAGCTTTCCAAGACTCTCTTTCCTAGAAGATGACTAG CTTCTGTGAAGCTTCTATTCAGGGAATAGCTAGCCTTCCAAGACTCCTTTTTTTGGAGAACGACTAGCTTTCTG AAACATCTATCCAGCTATAGTTAAAAGAAACTTCCAAAACTTTAAGTTAAAAAATTCTAAGTATAATGACTTTA TAAT G GAGT T CAAACTAACAAAAAATATATAAAAAAAAATTGTGTTTACTAAAAT CAAAGAAAAGAT TAAT TAA GT T GGAAATATAT TACT TAGAGTAGCAT GTAT TATAT GTACAT T CAAT T GT T GAATAATAAGT CAGT GT T GT T T GGATAACATTGCAATTTCAATTGCTTAATGCCAATATTTCAAAGAGATGTATAATATTCAGTTTTTGTTTCTCA GTTGGTCTCTTCCTCAATAGCTCCATCAATTATTATCCTATACTCATGATTAGCACTGTCTTTGTTCAAAAAAA AAAAAAGACCATATACTAAATTGTATCATTTAGGTCCATAAATGTTACTTTTATTTTTTTTTTATTTATACTTT GACAGAGTCATGAACTAAGTTTACATAACAGTAGAACAGCATAAAAATTCCCAAGTCTTGGTTGTTTTTTCCAT T T GT T T T TAAATGAAAAGTAT TACTATAATAAACT TAT TAACATT C CTAAAGAT GAT GAAAGT CAATAGT CAAT AAT AAT AAT ACAT C GT CAT AAT GT T GT GAAGT T GAAT T T T T AAT ACAAGT GT AAACACAAGAAT ATAAT T T CAT CTTTCTAGTGCATTGCTGCCTAAACCATAAGAAGAAATAGTGTTAGTTGTGATAAAGACAAACACAGGTAAGAA TATTGATCTAATTATAAGTCTATCCTTTCTCATCTACTTTGGGTTGTTCGACCAGTTAAAAGAAGATTTGATAA GCACAACATAACAAAATAGTCAGTCAACACGGCACCAAATTTGTCAAACATTGCATATTTTTGTATGACATTTT CGTGTATTTTGTACACACTATTAATTAATTCAATATGAAGATCATGTTGTATATCAATGGCCATGAAAAAAGAA AACAAGTGGAGGAATCACATGAATCATCTTATTAAATTTGATGAGAAACACTTTCATGGTTTCTTATTTCACTT CAT T T T GT GAAATATAACTATACAT T C GGATAT TAT GTACAAAT GAAT GTAATATAC CT CAAAAATT GAAGC GA AAGAGGAAGAAAAAATCGCTGCCAAACCACAAGCCTAGGCACAAAATAAGTTAAACTTGGCCTTTTGTTGTTGT TGTTTTTTTGTTTATGAAAAATAGTAAGGAAGAAAAGGCAGGCAAATTTTGAGGCTGATATGCTGATCAATTCA GAAAATGACTGAAAAACCTTTCACATAATTGCACCTACTAGACTATATAGAGCCTCTTGATTCAAAAACCCAGT TGATGATAGTGAGTAGAGTCAATTTCATTCAGTCGAACGCAACACAAGATATGCTCGGGCAAATCTTCGGGCTT AGTTCCCTGTTTCAAAGCACAAACCAGTATCATCAGTTATGGAACTCAAGATGTCATGAAAAAGCTTTAATATA CCTTATTTGACAATTAAGTTACCTGAATTGTAAGACCAATATCCAATATACATAGCTTCAGTCTGTCTTGGAAT GCTTCGATACCTTTCCTCGAAATGTAGCTGAATCTATGCATATCTAGATCGATCTCCAAGTAGTTTTCTCCCTG TAAAGAGAGT CAGT CAACAAGT GT T T T GAGTAAGAT TATACT TAGAT CTAC CAT T CAT GGTAAT CAACAAAT T T CTATATGTAATTATGAAAACTATACCAAATAAAATTCGTGTTGAGGACGCGAAAGAACAGGTTTTTCATTGTAA GCATTCATAAGCTTCTTCTCAGCTGTGCTCAAATGAAGATCCTCTACATTTGCAACTCTTCCCAAAATTTTCAA TCTTTCCCTAAAGGGTGCAATTGTATCAACAGGAAAACCTCTTACTCTTTCAACTTCATCATTGATCATTTTCT AAAAAAGGAAGACAAAACAT GGCTAGT CAATAAAT GT GAGCAGAAGCCTAAAA Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 60555650 60560649 1 5000 upstream regulatory region gene 60560650 60561877 5001 6228 genebiotype = protein coding 5kbs downstream 60561878 60566877 6229 11228 downstream regulatory region mRNA 60560650 60561877 5001 6228 gene=LOCl 15700367 exon 60560650 60560782 5001 5133 product=transcription factor MYB35 exon 60560881 60561010 5232 5361 product=transcription factor MYB35 exon 60561097 60561274 5448 5625 product=transcription factor MYB35 exon 60561347 60561877 5698 6228 product=transcription factor MYB35 CDS 60560650 60560782 5001 5133 gene=LOCl 15700367 CDS 60560881 60561010 5232 5361 gene=LOCl 15700367 CDS 60561097 60561274 5448 5625 gcnc=LOCl 15700367 CDS 60561347 60561877 5698 6228 gene=LOCl 15700367 >MYB35-15 amino acid sequence from CBDRx (SEQ ID NO:15) MGRPPCCDKSNVKRGLWTAEEDAKLLAHVSKLGIGNWTLVPKKAGLNRCGKSCRLRWTNYLRPDLKHDTFTPQE EDLIINLHQAIGSRWSLIAKQLPGRTDNDVKNYWNTKLRKKLYTMGIDPVTHKPISQILSEFGNISGLPNNHHI PSTNMISNTTTTTTPARIFYNNNTNNIGTSHQFQVMTNQNLFNNNNNNNVQPHFFNEVTSPCSSSSSSHVTDLS SPQWLFAPPLPPPLPLPPCNSNNWSEFFLGDNTEDQAKENDLEGFLLSSSSSSHTNSSKLSNGNGSDQNGSNI QEGQFAQMINNDHHHHHLQPENSSFVDAILDRDSELRAAFPEFLDGSFEY See APPENDIX F for the four most common genotype sequences in Cannabis accessions for MYB35-15. Gene name: MYB35-16 CBDRx assembly gene ID: LOCI 15711316 Number of Isoforms: 1 Number of Indels: 2 Number of Exon SNPs: 141 Description of variation: The genomic region has individuals with large (e.g., -5 kb) insertions, which are adjacent to the gene start position, and some individuals with smaller insertions (e.g., -535 bp) in the downstream region. The large insertions make alignment difficult and add gaps in the exon only alignment. The CBDRx annotation is smaller than other assemblies; exon one starts -200 bp later than other assemblies and exon three ends -230 bp earlier. >MYB35-16 nucleic acid sequence from CBDRx (SEQ ID NO:7) GTGTCCGGATGAGTTTTAACCCGATGCCCAGCTCACATAAATCCTTCCATAAGACTCTTCTTTCAGTTCTATTG TTTGATGCATAAACCACAGTCAAGAGACTATTAAATCCTTCAGTTGTTGTCAGCCTTAAATGCATTAGTTGACT AGTGCATTTAAGGATGTCAACAGTAAATCGATCAGGATTCCAAGCGATGACATTCTTACCACCTGCATGCCATG CTATATTGGAAGAAAAACACCAATTTACAAACACATTCAAGTATAAGGCTCCAAGTTTCGAAGCCTTAACTCTT ATTTCAAGGAGACCTACAAAATCAACTTTCTGAGAATTGATAAATTGCTTTATCATTTGTTGCTTATGTTGACT GTTGGCTCCCCGAACATTCCAGGCTAACAATTTATCCATTAGACAGAGAGGGATCTCCCCCCCTCCTCTCTTGC ACAAAACATCAGCTTGTTCATGAACATTTGCATCAGTAACCAATTCCTCTTCCTCAAGCACCTGGAACATATTT CCCACTTCAGTGTTAATAGTCACACCAGCATCAGCATCTTTAGTCCATGTAGACTCTCGACTAACACTATTCTT CTTGCTTTGCACTTTAGTAAAACCTTCCTCATCCACTTGAGGTTGTTGTCTTTTGTAACTCTTAACAATCCACT TCTGCTTCCCATTGCTATTCTTTTTACACTCTGCAGCCGCATGCCCAACACCTGAACAATGTGTATAAGAGATA GGCTTCCATTCGTATTTAATCCCTACCGAAGTGTTCATGCCAAACTCATTCTCAAACTCCAATAGATCTGGCAG ATTTTGGTCCATTTGAACCTCAACTAAAATTCTAGGGTAATTCTGTCTCTCTCTTTCCCTAGTTACACTATCCA CCATAATCGGGTTTCCAACTTGAGCTACAATCTTGAAGAGTGATTTCTGACCCCAATACTTCAATTCCAATTCC TCCAGCTGTACCCAAACTGGTACCTTTTGAACATCTTCTTTTTTATAGTTTTCATTTGGGTCCCAAGGTTTCAT AATCACCGGTCTTCTATTGAAAAAATGTACCCACCATTTAAGACCTGATCCCTGTCCTCAAGAGAGTGAAATCG AATAATAAAGATGCCATAAGACAGCATTTTCACTCGATCAACTTTCCCCTGCCAAATCCTGTGCACAAATCCAT CCAAAACATGGAGTGGGGGATTCACCCCTAACACATAACATACTATGGATGATTTCCAAAAATGAATTTCATCT TCAATGTCATCCATTGTAATTCTCACTTTGTTCACATTCTGATTAACATGCTTAAAAGAATTCTCTAGGTTTCG AACAATAGAATTAGACCTCAGAATAGGAGGAATCGATTTCTTACCAGCTGCAATATCACTGTTGCATTGGTTGT TTGCTTGAATGAAGTGTGCAAAGTCATTTCGAACATCCTCCTGCCTCTTAATCGCATCCAGAGACGATTTTGGT GAGAGAGGTTGGAATATATCGATTGGAGCAGGGTCATCGTCTCTCGCCGCATCCTCATGAGAAAGCTCCAGGGC TTCTATTTCAGCAATTCTTTCCATCGAACCATCCTGTACCGCCTCAGTAACCTCCTTCCTTCCTACTTTCTTTG AGAGTTTCGTTGCAGATTTAGGTTTTCCAAGATTCTTGGATCTTGTTTTAGCCATGGCATCAGCTAAATCAGCT GCAAGAGCAGGAACCGGGAAAAACCTTTTTTCACATTATTCAAAAAAAAAATATGAATAGAAATTTAGCTATTC ACCAGAAGTTGACACGTGTATTACAAAAGGAATATGAATCAGGTCAAAGAATATTATAATTAAATTACATTATT AAATGCAATTTAAATAACTTATTTCAATTAATTAAAACTGAATAGAAATATTCAAACTTGCTGACCACATAAGC AT AT G C GAGAT AAGAAT AAT AAC TAT T AAC AGAT GT AAT T AAT GAGAT AAT G C GT C CAT CT C AG GAT AAAGAT A ATATATAATAAGAGCGCACAAGATGAGACATCAAAGAGCTCGTAGCTCATTAACCCACATACTTGGGAGTAAAA GCTTGCAACTCATTGACCCACATATTTGTGCATGGAAGAAATAACTACATTCATCCATCTTGCCCTTTACATGA CAAAGTAGCCACCCTGCTAGTATCATTACAATATGAAGAATACAGTAAGGAAAAGTAAATTTATACTTAACATC TTTTCCTATAAAGATGGTAGGAAGGATAACTCACATCTAGCGAGAAACAACCTTCTTAGTCAGCATAACCGTTT ATAAATAAAGATAAAGAGTAGTTGGGTAGTTATTAGATATTATCCCATGTGCATAAAAATAGAATGTGGGGAAT GT C CAC GT TT CAT GAACAAT T GT AAAT GAATAGAT T C CAAT AAAAATAACT GT CAT C CT AT ACACCT AT AAAT A GAGGCAGAATCTAGGGAAAAAGAGATGAAAAACTTAAGAGAAAAGACTATTATTTATATTTATTAAGAATTGTA CTCTGTATCTAAATAAATATCCTGAATAAGAAGGACTTGTGGAGTATGTAGATTTTAACTACAAAACTACGTAA AATATCTTGTGTTCATTTATTATTATTAAATTCTATAATTTTTTATTACGTAAAAGGCTATATATTTAGCTAAA AAAAT GT GTTAACAACAT C C CAGACTAAACAT GCAAAATAATACACAAAAC C CAGAAAATATGAT CCAGAGATA AAAGAAGTATGATACCTGATGTGGAAAGTCGAGATGTCAAACTGATAGACACTGGGTTCAACTGGGGAAATCGA TCGCACCTTTCACAAAGAAGGGATGCTAGGGCGATCAACGAATCTATTTTTACGTCAAAATCCAGAATCGCATC TTCTTCTTCTGGCGTAAAGCTCACGATCACTGGGGGTTAAAGATGTGGAAGTTCTTGCGCTTGAATCCTCAATA GTAGATGAATGCGTGTCAACTGCACATTCCAAGGTAAAGAAGGAAAGTTAGCTCTGGACTCGGTTCTAGTTCTC AAAGAGCGTTAAGAAAGTTAGAAGCTTTGAGAGAGTTGAAAAGTTGAAAAGTTGGGAAACGAATGAAAAGTGGT AACTGGAGTTCATCAAGAGTATTTATATAAAAAAGGAGTGATCCACGTGAGATGGACGGACCATGATCCTAATT CTAGAAGCACTACTTTCATCCGATGGCCAGGGGGTTCCACAATCCACGCATATCCTTGCACTTAAAGTTCCCCG GAATTAAGGCACATATCAATGATTGAGATGCCCTTTTTTCACGCCAAAAACTAAACAGCCGTCAAAATATAACT TTTGAAAAGTGACTCCTCGATGGTCGCGCCCTTCCATCATCACGCAATCGACACGTGGATGTACTATCATCATA GGCGGAAGCTATCTTCCAAGATCGAGTGGCACTCAGAAATTCATGATTTTATCCCTCAACCTCCACATGTCCAT ATGGATGGAGTGATGCAAGCATCCAGCATACGCAACATTCTGGAAGCTTGGGAGGTAAATGTTATTTGCGTTTT CGACAGTGGACATGTGGAGTCCAGGTCAGCATCCAAGGCCCATTAGTGGGTCCCTCAACAAAGGTAAAGTGTGC CAAGTCCATTTGGATAAGTCCTTCCATGAACATACCTCAGTTCCGTAATCATAAAACCTTCTGCCTTCGGCGAG CCGTGCCCAAAAGCATGTCTGATATCATTAGACTGGAGGGACGAGAGAGCAAGTTCCTAGAAGACCCTTCTAGT TTTCCTTTTTATTCTTGTCCATAAATAAGGAAACTCCAGCTGCCAGATCACAACAAAGTATCGTATCAGGATTG AT AGT GAAAC T T C C T AT AGT GAAC AT T AC AC AAT T AAT T CAT AAAT T AAT T T T C CAT AAT T AAAGCAC AC AAT G TTGACCAAATCAAGCCAAATCCGATCCGCCTAGTCAGCAGATCGTCTCCTTCGTGGGCTGGACTTCATGAGATG GCCCAAGTGTATTATTTACCATAAACTCTCTAATAGAGGGAAAAGGGAAACTGAATATAATTCTCAAACTACTA TAAATACCCATCGAATCTTATGGATTAAGGGTTGAATTCTCTTGCAAACTTTGCTCAAGCAACTTGACAACATA AATTCTTTATATTCTCAAATGCTTTTTCAAGTAAAGAACTACTAAATAATACTGACTCGTGGACTTAGACTAAT TAACGCCCGCACAAGTAAAAAACTACTTGTCTCTTTTTACTCTTTCTATTTTAATTATTTATTTCTTTAGTTCT TAT CAT TAATATT CTAT T T T C GAAAAT CT CAGTAAATAC CTATAAACT CATAAAAT C C C GAT CATAAGAT GAAC TCGTAGACTATGAATAACTAACTTCAAAATCACATAAAAATACCTTTTTGTTTAATTATTATTATTAATGTCTT TTTGAAGTATATTTATTAATTTTAGACTCATTTATTGGTTAACGAAAATTGGAATTAACACGTATATATGTTGT TGTACATATACTATAAATTTTCTTTTTATTATTTTATTATAATAATTATCTTTAAATAAAATTATTTTAATTAT GTTCAATTAAAATAAGATTTTTCTTAAAATTTAAATTTGAATTTGAAATTACTTTTAATTATTTATGACATCTT ATGTGTTAAATTTAATTAAAAAATATGTGTTTTATTATTTTATTAAACAAAATGAGTTCTCGCGGCAGCACCTT GCAAAGCTCGGCTGATGTTAATACATAATCCATATAATAACAGCATTAACTGCATGATGTCAATATCTAATCCC AACAGTAGCTTAGTATTTAAACTCCATTACGCTAATATTCAATAAAAACATATATATATATACTCCATATATAT ATATCATTCATCTTCTTTATTATTCTTAATAGATTGCAAAGCATTTAATAATCCATCAAGTTAAAATGTAGTCA CCAATTTGTTTTTGTTTTTGTTTTTTTCCCCTTATAAAAAAACTGCATGAAAAATAAAAACTTTTACTAGCTAG CTAGTACAACGATTCAGCTAAAAACTCAGGAAACTCATCAAGCAACATCTCGTTCTCTCGAGCTAGCAAGGCTT CCACAAATGAAGTATTATCCATATCTTTAGATCCTTCACTCTGATTTTGGCTTAGATCATTCTGTGAAGAAGAA TAATTCATCAACTCAGCCATTGATGTCTCATGTTCTTGAGCTTGATCATTTGGCAAAAATGCATCTTCAAGAAG AAAGTCATTCCAATTGAAGCTGGCTACACCAGCTAGAGGTGGCATCTCTTGGGTACTGGATGAAGATGATGATG AGGGTGAAGAAAATGTTGCTTCTGGGTAGAAACAAGGCAATAATGAAGATTCATAATTATTGGGTTTGTAGTTT GAAGCTTCAGTACTGACCAACTTTATGGCTTGGAGCTGGGAAAGAAGATCGACTGATGAGATGTCGTTTGATGA AATATTGTTGTCGTTTGATAAAAATCTGTCTAGTGCTGGTTCTGTTTTGGGTGCAATATTGGTAGTCATTATTG TTGTGGTTGCTTGATGAGTTAGCATGAAATGTCTGGTTGTGGTTGTATTTGGCAGTGGTGAGAGTGAGCCTTTT GGATCCATCTTCATAAGGATTGCATTCTTTAGGTCTTTGTTGAGGGAACCGATTCTCATGCCGGTCTTTGGGAG GCCACCCACATTTCCATAGTCAGCTATGATTTGAGAGAAAGGCTTGTGAGTCACTGGGTCAATCCCCATCTCAG ACAGCTTCTTTCTCAGCTTAGTGTTCCAGTAGTTCTTCACATCATTGTCTGTTCTTCCTGGAAGTTGTTGAGCT ATCATAGACCACCTATAAATATACATATATGTATATATATAGAGAAGGCTGTTAAAACAGTTGGGAGTCACTTT ATGCGCATCATTGGTGGTTATCTACACAATGCTCATGTGGGGTTTGAGGCCTTTGTTCTTAATTTTGTACAATG CAATGATCTCTTTCTTTTTCCTTTAAATCATTTCTTACAGAATTTTTTGGGGGGTCCAATTAATTCCAAAGAAA AAAAGCAAGAAAAAAGCTAGTTAAGCAAAAACCAAAAACAAAAAGATAGTGGGAAATTAAGGTTTGGAAATGAG AGTAAAAATACTACATTATTTGCTTCTCAAAGATATTCATCCAAATTATCTCAAAAAAAAAAAAATAGTGAAAT TAAAATGGGTACCATTAAAAAAAATGAGAAAGAGCAATGGCTTTGGTTAGATGTTGAAAGTCAAAATATTACCT GCTTCCAATGGCTGCATGAAGCTTCACAATGAGTTCTTCTTCTTTAGGTGTGAAGCTCTCATGGTTGAGATCAG GCCTCAGATGATTATTCCATCTAAGTCTACAACTCTTCCCACATCTTCTAAGTCCTGGAAAAAAAACACTCACA AACATCCATTCCAAAACTAAGATTTTATTCTCATTTAATTATACACCAATATTTTGCTTGAAAAAGCTAATTAA T TAAC T GAAT C T T GAT AAAAT AT AT AT AT GT AT AT T T T T AT AT AAAT AAAAAT GAC AT AT AT AAGAC C T GAAAA AGTACTCAACCTGCTCCTTTAGGCACAGAGGTCCACTTGGCTTTTCCATGTTTAGATACATATTCAAGCATTCT TGCATCTTCCTCTTCAGACCAAAGACCCCCTTTCAAGTTGGCCTTTTCACAAGAAGAAGGCCTCACCATCTTTA TATATATTGATAATCTGTTTAATACCAAACAAAGAAACAAGATAGACACAATTACAACTTAACCAAAATTGGGT T T TAAAAATAAAATAAAAAATGAAT GAAAATTATAATAT GATAAAAAAAAT TAAAAAAAATAAATAAAGGTT T T GATTCAACTTGAAACTTGATTTTTGAAACTGCCTCGGTCTGATATTATTTGAGAAGATACGAAAATTGTTTTTC AGTGGGTTTACAAGTTATTATGTAGAGGCAAATAAAGAATGGGGTTAATTTTGAATGAGTATTAACACATCCTC ACACTAATTTAACTGTTTTGATATTAAGAAAAATAAAATAAAATATACAAATTAATTCTTTCTTTAACTCCACC ATAATTGCATGCTTTTGGCTTCAAGAGCATTTTGAGTGATCAGGTTCAAATCGGGTGAATTATTCGGTTAAACT AGATTAAATCTAAAGTGAGATTAACATAAATAATTCGATTTAGTTAGATTAATTTAGCTACTTTTTTAAAATAT AAT T T CAT GT C T T C CATATAAAATT CAAGT TT TAGT TAAAAGAAAT GACAAGAGAT T GAT GAGAGT CACTAT T T TATTAATTAAACAAATTATTCAACTATATTGTTGAAGTGGAAAAAAAATGGCTTGCAGCACTCATTTTAGGCTT CTTGAAAAAGAGGAGCTAGCTCTCATTTTTTTTTTTTTTTGCTTCTGATTCACTTATTTTATGTATATTTTCTC CATTAAGGATGATGAAGATAAATAACAACAATATATTAGAAAATTTTTATTAAGTTACTAGAAAATAATTCACT T GATATAT GCAAGAAAAT G GATAT GAG GTAT GAAGAAT GAAT C CTAAAAATATT T GAAT TT TAAACAAAGT TAA AATTATTTAATTATTTAGGGATTATCTTAATTGTTATCAGAAAGTGAAGAATGTATACAAGTGGCAGTGTGGCA CACATTTGTAGCTTGGAAGCCATGGTTTTTATTTATTATTTGAATAATTGAAATAATAATATGAATACAAACAT GAAATAAGAAAAAGGTCTCATTTACTTTGCTACTTTGTTTTTCAGATTTATTCATGTTCTTAGAATAGCAGTAT TTGTTGAAACAAACACTTTCTTATCTTAATGACCATAAGATGGTTCACCCCTAATCACTTTAGAATAGCTATCA AACATGAAAACTTAAGTTAACAATTCTAGCTTCTCTTAAGTTTATGATCAGGACATCCACGATTCTAGGCTTAG CTTTTCTCTAAAGGGACTCAGGCAGCTGCTAGTAACTAATCATAGACCTGTCACTTTCATCAATATGCAACTCT GAAGTTCTTAGGAGGTAAGTTTGAATACAATTCGAAGTTCAACTTTATGATATTTGAACTGCATAACTAAATAT TTCTCCACCGCTATCAGTTTACAAGATCTTTAGCCACGTACCTTAATGGTTTTCACCATTACTAGAAATTCATG AAATTTCTTAAATATTTCAAATTTCTTTATTTAAGGTATAATCTAGAGTGATCGTCTTAAGAATTTAGCGACAA CTCATATCCACCCCTGAATGTACATCCATATATTCGAATACAGATGATCCTACTTCTAAGTGGATATTGGCATA TTTTTTCTTTGCAGAGACTGATCTTGTCAAAACCATTATGAACAAGATGCAAATGCCATAGATATATTATTGTG GTTTGATGAATCTTAGGTCTAGTTACAACAAAGAGTTCTTAGAAAGTGGATTCTGGTCATAGAATAGAAAACTC ATAAT C CATAAATTAACATACAGT T C GGAT C CAT T GATAGAAAAT GGATAT TAACAACT T GT GAAAGT T T GACT GTATTGTCCAATGTATTCTAGAAACTGAAATTTAAAATTTCTATTTGGAATCTAAAGTTTAAAGTAAAAGACTT AAATTTATACTAAATATTCCTTGAGTAATTATTCTAGCTTGGACCACCACTACTTGTTAACGCAGATTTTAGTT AACTAT CTAT GAGCCTACAGT GATAAT CT CACATAAAAAAAAATAAAAAACAGCAAAGGAAAAAGTATAGACAC AAAGGATTTTTTACGTGGTTTTGTAGTTAAAATTCTGCATAGTCCACGAGTCAATCTTATTAAATTTCTGGTAG TTTTTCAGGGCCTTTTTCTCTTATGATTTTTTTCGTCCCCTTTTTTGATCTAACTTGCCACTATTTATAATCAC ATAGTAGGCAGTTAATTACAGAGTTGACTATAATCTTTACAACGTTTATCCCCTGAAATCATGGGGTTTGATTA CATATCACGTAGTATAAATGCGGTTATTATTTGAATATCACACAGCTGTGACTTATCCGCTTTTACTGGGTCAA AATGATCGTGTATTTAAACACATTTTAAATCACATCGTTCTGGGATGTCTCGACGGGAACTCAACATTAATGAC CTTGATGGCGAGCTGGAATGTTTCCCCTATCCTTCGGAAATTGACGATGTACGCCTCGTTGTTTCTTTGCTTGA AGGCATAACACTTCATTTTTGACAGTCACAGTTCTTTATGACAAAAAGAGTACTTCAGTAGCCATCATTCTACT GTCAGCTTCTGACTCGTCTAGACTATAAGTCTCACTGAAAGATGTCTGAAGTTGATTGGTGAAAGTAACAGGAG TGCTCCTTCCTATAGCGAGATAGGCACCTCGCTTAATAGGCCTTGAGTTTACCCAACTGATATACTTAGTTTGT ATATTATGTGCTAAGTGTATTCACAATCGATATGCATCGCTTTCATCTCCCCAGGGGTAACGAAGTTGATGTCT CGCTTTATGCATCCAATGACGTATGGTTTCTCGTTAATACACGAGGCAACAGTTCATATATCTTTAACCTCGCA TAAAACCTTATAGTCAGCGAGTTGTAAGTATACTCTGTTACTTACACAATTAATGAGTTATCCCATTAAAAGTA ATTTCATCGATCCATATTCCCCTTAGCTTGACACGTGTCCTCTATTGAAATGGTAGCCGAATTTCGGGTATAAC ACTACTATTTTAACTCTAAGTTTAAATTTCCTTTAGTAAGCATATACAAGTAGGAGATGTTTCTAAGATTGAGG AGTTATATCATTACGGGATAGAATATATGGAGTGTAGTCATCTGCTCCACTTAATTTAATAGAAGAAAATAGAG TGACATCACAAGTAGATGATTTATAAACCTTCTTTATCCAATGATATACTATCTTATTAAATCTTTTAATGAAA AAAGCTAAGAGGAAACTAAAGAATAATTTT GATTAAAACAAGAAT CTAATAAT GT CT CATTTAGGAGAGAGT CA AAGTTATCTTATTTTATAGACGTCTTCATTGTTTCATATTGTAAACACAAGTCTAAGTTGCATCTTTAGGAATG AGTCAATTAGATGTTGCATGTACAAATATTAATCTGTCGAGATCTAACACTATTATGTTGTCTAACGGATGACA ATCTTTAGGAGATTGTCCCATTAAAATAACAAACCAACTTAGACCAACAATTAAGACTCAAAATTTATACTACA T GAT TAATAAGAGAAAAAATACAG GT T CAATATAAAT T TATACACAAT TAAAAAAT TAT TAAATAT T TAG CAAG T AAC AAAGAC AT GT GAAAAT ACAAAAT AAAC AT AT C T T AAAT AAT T T T T GAGAT T T T C AAC AAAT C GAT AT TAG TGTCCCGTTTAAGCGAGAGTCAAAGATATCATTCATTAAATAGAGTAGTTAGCTCATCTAAAATGGACACCGTT TTAGCAACCTTTTAGTCAATGAAAATGAGGATCAAACGTTGTCCCGTTTAGGCCAAAGTCAAGGTCATTCTCTT TCATGAGCTTCTACCATTGTTTCATACTTTGTAAGCACTACTCTTAGCAGTCACTACTGGGGTGATTGTTACTA AGAATAAAAGATTAACAATAATACTTATCATTCGAGATTCTACGACGTTTAATTGCTAATGAATGGACATCTAT TAGGGATGACTATCATTCAAACAAGAACTATGTAGAACCAACAATGGAGATCGAATATCCTTAAACTAAAGCTC GT T AT T T T AAAGAAT T GT AT T T T CAT T T GAT AGAT AT AT T T T AAT C TAT T AAT AT T CAT AT AT T TAT T T AAT TA AAATTACTAATTTAGATAAATCTAAATATAAAATTTACTTTAATATTTATAAAACTATATACATACTTGGATAG AT C T AAAAAAAT AAAAT AAAT T T AT T T T C CAT CCTTTGTAT T AAT T T T T AAT T AAC AT TAT GAAAAT T AC T T GA TTTAAGTTAGTCCAAAATTAATTAAAGTTAATTTTCAACTCAAATCTAATTTTCTATAAATGCATAACTATATA ATCATAACTCAAAATCTAATGTATTTTTTTTTTTTTTGACATGAAATCTAATGTATTTAAAATACATTTTCAAA T T TACAT TAAAATAAAATAAAATAAATCT GAAAGAAT TAT T CTAAT TTATGTTGGTT TAAAATTAAT TAAGAAA TTAATTTTCAACTTGAATATAATTTTCTTATTTAATTAAATTTCATGAAAAACTTCTAATGTTAAAGTATCTTG AG CAAAT CAAATTAAATATATACAAAAAAAAAAATAC CAAAGT CTACT TAGAAAATAACAAT CTAGATAT T T C G TAAACTAATTATTGATTTTCTAATTTAAAATATAATATATTTTCGTCTATTTATTTTAATAAATCATTAATGAA AGAT T GT T T GAT TAAGT T GAT T CAAAATTAAT T CAT TAAT T T T CAACT T T GAT C GAACT TT CAAAAT T GAAAAT T T T GAAATAT C TACAT T TAAAAT TAAAT GTAAT T CAAAAT T TAG GT CAAAAAAT GAT TAT TAAAATAAAATAAA ATATACTCTTATCATTATTCTAATTTAAGTTGATCTGAAATTAAAAAATTTCATCATAAATATAACTTTCTATT T AAT T AAAT T T AT T AAAAAC AAAAAC AAAT AAT T AAG Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 83249697 83254696 1 5000 upstream regulatory’ region gene 83254697 83256426 5001 6730 gene biotype=protein coding 5kbs downstream 83256427 83261426 6731 11730 downstream regulatory-region mRNA 83254697 83256426 5001 6730 gcnc=LOC115711316 exon 83256294 83256426 6598 6730 product=transcription factor MYB35 exon 83255986 83256115 6290 6419 product=transcription factor MYB35 exon 83254697 83255555 5001 5859 product=transcription factor MYB35 CDS 83256294 83256426 6598 6730 gene=LOC115711316 CDS 83255986 83256115 6290 6419 gene=LOC115711316 CDS 83254730 83255555 5034 5859 gene=LOC115711316 >MYB35-16 amino acid sequence from CBDRx (SEQ ID NO:16) MVRPSSCEKANLKGGLWSEEEDARMLEYVSKHGKAKWTSVPKGAGLRRCGKSCRLRWNNHLRPDLNHESFTPKE EELIVKLHAAIGSRWSMIAQQLPGRTDNDVKNYWNTKLRKKLSEMGIDPVTHKPFSQIIADYGNVGGLPKTGMR IGSLNKDLKNAILMKMDPKGSLSPLPNTTTTRHFMLTHQATTTIMTTNIAPKTEPALDRFLSNDNNISSNDISS VDLLSQLQAIKLVSTEASNYKPNNYESSLLPCFYPEATFSSPSSSSSSSTQEMPPLAGVASFNWNDFLLEDAFL PNDQAQEHETSMAELMNYSCSQNDLSQNQSEGSKDMDNTSFVEALLARENEMLLDEFPEFLAESLY See APPENDIX G for the five most common genotype sequences in Cannabis accessions for MYB35-16. Gene name: MYB80 CBDRx assembly gene ID: LOC115725196 Number of Isoforms: 1 Number of Indels: 3 Number of Exon SNPs: 50 Description of variation: The genomic region around the MYB80 gene is characterized by a few different insertions in the upstream regions. The MYB80 gene and the downstream regions are well conserved. The CBDRx annotation and the other NCBI assembly, pink pepper (GCF_029168945.1) have an additional exon at the beginning of the gene. This first exon starts -640 base pairs before the start position in other assemblies and ends -90 base pairs before the start position in other assemblies. The second exon in CBDRx is smaller and starts -365 base pairs later. The final and fourth exon ends -115 base pairs earlier than other assemblies. >MYB80 nucleic acid sequence from CBDRx (SEQ ID NO:8) GACACAT GT CAACT TTATAT C CATATAGT GC CAAAC CATAATAATAATAATAATAATAATAATAATAATAATAA TAATAATAAT GGAGT GT T CT TAT CAAT TAT GAATAT T T T TAT T TT T TAAAT T TATATATATAAAAAGAATAGT T AGATTTTTTTATAAGTTATTCAACGTTTCATTTGAATTTAATTTAAAGATGGTCTCTTTTAATGTTGGTTATTT TTTGGTGTTGTTTGTTTCTTAGATAGCATTTAAGTATTACAAACCAAAATTTTAGCTCTTTTGGTAAGATTCAA TTGAACTTATTTGTGCAGAATCTCAATTAGAGATGTTTATTTAGATTTCACTTTCTTTGGTATCAACTATTAAC AAAAGAATGTATTTTTTAATGAGCTAGGAGTACTGTGTGAGGATGTTATACAGTTATAATTTTATTGTCTAAAT TTTTGGGAACAATTTTGTCCCATGTAGCTCGCGATTAGAATTATGCAGCTCATGGTTTAGCAAAACATGCATTT CGGTTAGACGATAAGCTACCCTAGCTCGAGGAGGTGTCACTACCAATTGTTATAAATTCTACCTCACAAATTTT TCTGCTTATTAGAAAATGACTAAAGTTTAGGGGTACGATTTGATACATGTCAAAGTCTAGAGTGGACGATTTGT TATATGTCAAAATTTGGAGGACATGATCTAGTATATGGAAAAGAGAATTGGTAAAAGACACCACTGGTGTCTAT CACCCTCCTTAGTATCATACCGCTATTTGTGTAATTAAGTATCAAATCTCATATAACTTAAGAAAATAACTTTT AAAAAATAT C GATAACTAATAGT GAGATAC CATATATAAAAGGT GT TAGGTAT TAT T GGT GT T CAATAACAAT G GTCTATAGAAAATCATCACATTAGGCATTCGTTAATAAAATTGAATGAAATTAAATAGAATTTTTTTGAATCCA ACAAT CT CAAT AGT T CAAAT AACACAATT T AAT ACAT GT CAAAGT T CAGAAAAT ACAAT CT GAATAT AT AGAAA AT CAT CACAACAT T CAT TAATAAAAT TAAAT CAAAT TAGATAAAAAAAAT T TAAAT C TAATAAT T T TAAT AAT T AAAAAACAAAATTTAATACATATAAAAAATTCAGGAGCAAAACAAAAAGAACACGTGTCAACTATCTATGTAGA GTGCATTGTATACCTTAAAATTAAAGTAAAAAATAAAAAAGAATGATGTGTGAGTGGGTACTATGATTGGGCCG CAAAGTCGTACAAAAGTAAGACACATGGAGTTAGAAAGAGCCGTACGACTCTTTTACTCTAACAACCTAAGATC ACGACACGTGTCACACCACCACCCCTTTCTCACTATAATATATATTATATATAAAGATTGCATATTTATTTGAC CAATATCATTCTTTCTCTCCCTCTGTATATCATTATCGCCATTTTTGTTTAAATTCAAGTTTTTTTTTTTTGTT T T T C AT AAAC AGAAT AAAC AT CAT AT CAT TACT CAT T AC AGAGAC ACT GAGAT AAAGAC AGAGAGAGAGAGAAA GTTTATAGCTTTTTCTAGAATATACTTCAGATTACACGTATGTTCTCTTTTGTATCCTTCCAATCATTATAATT AATAGAAATTAAT TCTTTGTC GTAT GAT GAT GAT GAT GAT GACT CATAT CATATAT CTATATAT CT GT T GAGT T GTGTGTGATTGTATGATATAATTAATCTCAGGTTGAGACGCCATGGTTGGAGTTGGAGAAGCTCATCATTCTCA AAACACACCATTATTACCTAAGTTTCCCCGTAAAAGAACAGGTAAACTCAATTTTCTATGGTTTTTCTCATCTG GGCAATT CCAATATTGCGTATCTATTTATATATATATATATAT GATTTAAAT GATTT GATAGTCAAAATAATGT ATATTAGATGTTTGTTTGTTTGTTTGTGTCTGTTTAGTTTTATCTGATTATTAAATTTGAAATGTTGAAAGCAT ATTAGGTGTTTGTTTGATTGTCTGTTTAGTCATCTGGGCAACTCTCTCTCCCACATTTCATCACTCTTTTAATA TGTATATGTATATATATACACATATAAACATGTATATTAAATGTTTGTTTGTTCATTTGGGCTGAATTAATTGT CAGTTTAGTCATCTGAGCAACACTATCTCAGATTTCATAACCCTTTTAATATGTTAACTTTTGAAAAGTTCTGA ATAGT T TAAAGTAC CT T T GAT T GGT T TAAAGT T T T T TAAAACT GAAATAT CAGAAACT T T GT CAT CAATAAT GT GAACTGTGCAGTATTTTTCATCATCAGTAACTTTTATATTACTAATTAGTGATTACATTTAGATGTTCAAGTTC AAAGTTTTTAACTTTTTTTCTTTTTTTGAATGTTTTGAATTGTGAAAAGGGAATGTATGGACAGCTATAGCACA TATCATAACAGGTGTAATAGGCTCAGGGGTATTATCCCTTTCATGGAGTATGGCTCAATTAGGTTGGATTGTTG GTCCTTTAGCCATGGTTGCCTTTGCAACCACAACCCTTTTCTCAGCATTTCTTCTCTGCAACTCTTATAGATCA CCCGACCCCGAACATGGTCCTACTCGAAATCGTTCTTACATTGAAGCTGTTGATATGAATTTGGGTAACAAAAC AAATTATCCCTTTTCTTTACTTGGTTGTATTATCTAATATAATCCAATAACTCATGGCTTCTTGGTGTTGATGT GATTTGTAATGGTTTTTGTAGGGAAGAACAATTCTTTAGCTTGTGGCTTTCTTCTCCAGTTGACCATGTATGGA TTTGGCATTGCTTATACTATAACATCTGGCATTAGTATGAGGTATGGTGTTATTATGCTTATGCTTTTTCACGA TGAAGACTGCTTCCGTTAACTTCCTATTTTGATCTCTTTGTCAAATTTTACATCTGAAAACATATGTCAAGATT T TAT T T CACGGTAAT GT T CATAT T GT TATAATAT C GTAAAT GT TTAAAAAAT T C C GAGTAAT TTACAT TAT T GT CAATCTTCGCGTTTGTAAACATATGAAAGAATATTCTTTTTTCTCTTTTCATGACGGTGTTTGTTATAGTTACA TTGTCAAACTTGCAAATTTTTAAATCGGTTAAAGTTACTGTAAATTATTCGAAATTTTTTAATTTTTACCGGAA TAAT GT T GTAACTATAACAAATAC GGT TAT CAAAAGAAAAAACAACT CTAGCATAT GT T TT T GAAGTAAAGAT T GTAATAAGAGATTGTAATTAGCACTCATCTAAAAAGTATCACTTTGTTTCGATACATATTTGGATTTGGTTTAA AACTTTGAAGTTCATATTGTTAACATTATTCTATTATGATTACCAGAGCAATTCAAGAATCAAATTGCTACCAC AAAGAAGGGCATGATGCTCCATGTACATATGGAGATACTTATTACATGTTACTATTTGGAGTTGTTCAAATAAC ATTATCCCAACTACCTGATTTTCATGGCATACAATGGCTCTCAGTTGTTGCTGCAATCATGTCTTTCACCTATG CCCTTATCGGATTGGGATTAGGCGTCGCAAAAGTCATCGGTATGTATGAATGAATGTGATAGAATTGAACTCCT TTTTCACCTCATTTCATGTCTAAAAGTTTGTCATTCTATGAATAAAGGTCAAGGCTTTATTGAAGGCAGCATAA CAGGAATTTCAACAACTAATCCAGTTGATAAAATATGGTTGGTATCTCAAGCCCTTGGAGATGTTGCATTTGCT T AT C CAT T CT C CAT AAT T C T C T T C GAAAT T C AG GT AAAAAAGAAAT AT T T T AAAC AC AT AT GAGAAAC AAC AT T CAATGTCATTAACCCTTTGGCTTTGAACAACAAGAGAGGCTGAAACAACATTTTTTTGCTGAATTTTGAAGGAC ACTTTGGAGTCACCACCACCCGAAAACCAGACAATGAAGAAGGCGTCCGCTATATCAATAATCGTTACAACATT CTTCTACCTCTTCTGTGGAGGAGCTGGATATGCTGCATTTGGTAACTACACACCGGGGAACCTAATGACCGGGT TTGGATTCTACGAGCCATATTTGCTCGTTGATTTAGCTAACATCTGTGTTGTTCTTCATCTAGTTGGCGGTTAT CAGGTATTTTGCAAAAGTTACTAATCAGATCACTTTCTGCTTAATTGTTATCAATTTTATTTTTAACATGACAA ACAGATACAAAAAATGTAACCAACCTGATCACAATAGAAAAATTCCTTTTTTTCACTAATTTTCTTTCTTTACT TAATGTGAATTTTTCGGTTTTTATTGGCAGATATACAGTCAACCATTATTTGCTAATGTAGAGCAATGGTTAGC TGAAAAGCTCCCAGAAAATGGAGTTATGAACAAGGATTACAGACTCAAAATTCCACTAATCTCAACTTTTAGAA TAAACCCTCTTAGGCTGAGTTTCCGAACAGCCTATGTCCTATCAACAACCGTGATCGCGATGATCTTCCCTTAC TTCAACCAGATTCTTGGGGTGTTAGGAGGCATAAACTTCTGGCCATTGACCATATATTTTCCAGTTGAGATGTA CTTGAAACAGGCAAATGTTGAAGCTTGGACAACAAAATGGATACTTCTTCGGGTTTTTAGCACCGTTTTCTTGG TCATAACGGTTTTCGCCTTGATTGGATCCATCCAAGGAATAATTAGTGCAAAGCTAAGTTGAGAAAACATTAAG GTTTTTATTTCCATTTGCATAGTTTTTCTGTTGATTTAGGTGGATTGTTGTTAGTTGGTTGCTAAGTTTTTTTT GAAAAT GCAT C CAT AAT T AAGCAT T T T GCATACAT GT AAGACATT T TAT GT AAT AAGT T AAT AATAT T T T T CAT GAGTTTAATGATTGATTATAATATTTGTACGGATACAAGTTACAAGCAAACAACCTTAAACATTTGCAACTCAA ACAAAGGAGAGCTAAACTAAACTCATCACACTACTTAATATGCCCGAGCAGCATTACTAGTTTCTCGATTGGCT ATCATCGAAATAACCAATCAAAATATGCCACGTGACTTGGAATGTTCAACATTTTCAGGGTGATTAAGGTTGTT TT T GT GT GAGAAAAAGAGGAAAGTCACCCAACAAT GGAAAT GACAT GTAT GCCATATAT GAGAACTAAACTCAT CACTTGTCATCACCCAAGCAGCTCGATTGACCATCATCGAAACAAGCCAATTAAAAGGCGCCACATGAGCCTGC TCGGGGATGTTAAGCATTTTTAGGGTGATTAAGGTTGTTTTTGTGTGAGAAAAAGAGGAAAGTCACCCAACAAT GGAAATGCCATATATGCCAATACATGAGAACTAAACTATCACTTGTCCCCGGGCAAGCAGCTCGATTGGCTATT GAAGCAACCAATCAAAAGACGCCACGTGAGCTTGCTCGGGGATGTTTAGTATTTTCGGGGTGATTAAGGTTGTT TT T GT GT GAGAAAAAAGGGGAAAGCCACCCAACCAT GGAAAT GCCATATAT GATAGGT CAAAAAGAAGAGAGAA AGAGAGTTGACCAAGCATCTTGTGGGTGAGCTACCAGCTTATCTATCACACGCTCTATAGCGTTGAATTCTTTA ATAAAATCATGCACCTTTGATGCTTTCACTTTGTAATTTTAACCCTTTTTTCTTTTTAATTTTCTCATGCAGTC TTTATACAAACAAAGTATTGCTACACTCACTACTCCTTTTATAAATATTGGTTTCTACAAATCCTTTTTATCTC ATCACACCCATTATTCAAGAGAAAAAAGAAACTAAAACTACCTCAAAACAACACTTAGTTTTTCTTTTTTTTGG TTTTTTTTTATTGTTTTAAAGCTTTAAGATACAAACTTAAAAACCCTTTTTCGATTTTGTTTTATCTCTTCTGT TCTGTTCTACTTATTCGTCATTTTAGTTTAGAAAAGATGGGTAGGATTCCTTGTTGTGAGAAGGAGAATGTGAA GAGGGGACAATGGACTCCTGAAGAAGATAACAAGCTATCTTCTTACATTGCTCAACATGGTACTCGCAATTGGC GTCTCATCCCTAAGAATGCCGGTTTGTGCTCGTTTTTTTCCTCCCTAGTTCTTGTTTTTGCTTCTTAATTTCTT ATACATACTAATCTTTGTGTTCTTTTAATAAAGGTCTACAAAGATGTGGGAAGAGTTGCAGGCTGCGGTGGACC AACTACCTCCGACCGGATCTAAAGCACGGTCAGTTCTCCGATGCAGAAGAGCAAACCATTGTCAAGCTTCACTC CATTGTTGGCAACAGGTACAATCAACTTGCAATTGGATGACCATATCATATCATACAATTTCACCTCATAGCTT AACTCATCCCAAATTGACCATATTACAGATGGTCAATAATTGCAGCCCAACTGCCCGGGCGAACAGACAATGAT GTGAAAAATCATTGGAACACAAAGCTGAAGAAGAAGCTTTCTGGTATGGGAATAGATCCAGTCACTCACAAACC ATTTTCCCATCTCATGGCAGAGATAGCCACAACATTGGAACCCCCACAAGTGGCTCATCTTGCAGAAGCAGCGC TCGGTTGCTTCAAAGATGAAATGCTTCACCTCCTTACCAAGAGGAGAATCGATTTTCAACTGCAACAATCAAAT GCTTCGATAGGGAATGCAACAACCACCTACATGAATAGTAAGCAAGAAGAGCATCAAGACGACGAAGCCATTGA AAAGATCAAATTTGGTCTCTCAAGATCCATCCAAGAACCCGATATGTTGCCCCCGAACAAGCTTTGGGACTCAG CAACAACTGGACCAACTTTCTCGAGTTTTTCAGGCAATTGTAGTAGTGCGTTCCCTTACTCTGGGTTTCAGTAC GAACCATCTTCTTTTAGCAATGAAAGAGATGGATCGCCATGGAACCAGAGTATCTGTACGGGAAGCACGTGCAT GGTTGGAGATAGGCAAAGCCGCTTACCTGACAAACTCGAAGAAAATGGAGACGACTCGAGGACAGGGAAAGAAA TAAGAAATGATGCCATTTTATTTAACACAGACAGTGTCTTATGGGATTTACCTTCTGATGACTTAATGCATCCA ATAGTTTAGTTTTTAACAAACAAATAAAAAGGGAGAGAAATCCTTTTCCTAGGAAAATAAATTTCTAATGGTAG CAAATAATAATGATGGAAAGTGTAAATAAAGCTCATTTTTATAAACTCTGATCATAAGAATAATTCCAATACAT TATTTCTTTCTATCAATCTCAATCAATGTTTTATAATAAACTTTCTATTCTCTCACTACAAACAAATACAATGT AAAAATCAAGCGAAATACGACGACGATGAAGATGTATCGGCGAGAACCTGCAACAAACCATATAAAAAAAGATT AAGAAAAACTTTGAACAAACTAATTACAGTTAGATGTTGAATTAAAAGAAAACTAACCTCGAGTTGCTCTTCGG TAAAAGAATCCTTTTGAATATTCCATGTGTCTGCATGGGTGCGGCGGAATTCTGCAACGGCTTTTGTAACTGTA GATTTAATAGGAGAGGCCTCTCCAACAAAACGAGCTAGTAATGTAACATGCTCGGGCAACCAACTGCGAAAACA ACAAAAACATCGCTTATATGGTTAGAGAAGAAGAGAGATAAACATCTGAAAACACTATGCTTTGTATTACTTTA AACAATACAAACCTGGGCATGTCGTAAGGAGCAGACAACACAGAAGCTGCCAATGCAAGTACAGCACCGTGTAT GGAAGCTACCGATGAACTTGAACTCGAGTTTCTGCATTCAGAAATCACCAGTCTTTTTATGTACACAATACTAT TATATATTTTAATCATACAAGCATTCTAAACATATGATCAGCATATAATACCGTTGTTTCCTTTTCCGTTGAAG AGCACTTGCTCCCTTATAAGCTCTATCGCGAAAGTCTTCAGCTAAATCTTCATCCCCGCCCTTCATGAGACCTG CTAGAACCGCT GCAGCAT GCT CT CTTACCT GAAGCAGAGCAAAAACATTAGAT CAAGAACAT CCTATGAACAAA CCGAAATTTACAGCAAAGTAGAAAGATTATTATCAAAATATAAGAGAGCAAGGAACTTGCCTCGACTTGGTTGT CTACAAGTAACTTCTCAACAGTTTGCCAGATTTGTTGCTTCTCTATGCTCGAAAGAATGAAGGTGTGCCTATAT TGAGTTCATTAAAAGGGACGAGTCAAGATAAGGCAAATAGTACAAAAACTGTAAAATTTGCATTTCCACACTCA AATCATCACATCTTAAATAGAATGGCAATGTAGGATTCCTTCCGAGTACGATACCTGTACATAAAAGTTCTCAG AAACGTCAATGTTGCAGATCTCGTTCTCCAGTTGGAATCATTGGCCGAAGAAAGAATCACACTTAGAGCTTCCT GGAGTTGGGGTTCCCAGAAAACCCTCCACTTTAACAATTCGAAAGCTGCCTTCGCTAAGGTTGATAAATCTTTA TTTGATGTCTCCTGCAAAAAGCAACCAATACCTCTCAGATATGAAGAATTGATACCGAGGCAAGTGAGAAAGAT ACTTGAACCAGCCTCGAGGGTCGAGGATGTTTTGTTTGTTAGCCTTATCACATAGTCATTTCAAGGCTAATGTG CT T CAAACAAAAGCAGAAGCT CATACCAAACT GAAAAAT CACTTTAAGTTATAAACAGAAT CAAATTT GAGCT G TACGCACTTGCAATGATATTACAGGATAAAGTAGACCGACGATTACATCCAGCAAATACGATGATCTCCCAGAC TT CAGAGATGAGAT GAT GAAAT GGAAAAGCTT CACAAAACAGAAAAGTACAAT GTT CAGTAGAT GTT CGAAAAA GCTAAAAATATTCCATTGAAGGACTTTAGAAAACTCAGTACCGTTTCCATCCATTTGACATCATCTTGTGAATC ACCATTAAGATGCCCATTTTGAATTGTATTCTTCTTGGTCTCCGAATTGTCTGATTGATTGCTCTGCTGAATGT TTACTACCACCTCAGATGCTCGTTTTTTGAGAAACTCAATCCAACTTCTCTTTTGAAGTTGGCTGTCAAGATCC ATATTCTCGTCCCCGTCTTGTGAATGATTGTGACAAACTGATGCATAAAGTTGAATATTTGAGCACAACACAGA AAGAGTAACACCTATAGCTTCCCTTACCTGTTTCACACAATTCATATATCAAAACATAATTAATAAATACAATC TATCAACCAATATACATATAAATAGTAGAACATAATCGAAACTCGCTTACTTGGGCAGATGAATGGCACATATT AGCAAGCAGCTCTTCCAGAAGTTTAAAGTGGAGCCGAATTTCAGCTGTTGACATCTTTTGTGGGGATATTTCGA TAAGTGCAGCTGAAATAAAAGCATAGCGCTTGGCAACAACAGTAGTAGTTACCGTCGAAGGTAAAGGTGTAGCT AAACAATCGAGTATTTGCTGTCTCAGAAGAGGAATTCTAGTTCCATATTTTCCTTTACCAGTAACTGCGTAACG TATACAAGCTGCCCACTCAGGTATTGATTCAACCGATTGCGATAGAACGATATTCTGCAATTGGACCACCATCC AACTGTCCCAAGCTTCAACAAGGCCATTAACATCGGAATGCAACACACCAGCAAATGCTTCCGCAGCAACACAT TGCTTAGACCTCTCCTTTGCAAGCGCAAACTCTTCCAATGAGCTTTTGAGTGCCGATAAAACAGGCATGCCACA TTCTTGTACTAACCGCTTGAAAATCCGTGCAAAGTTCGAGTAAAAATTATCAGTACCTAACAAAGATATCCAAC TAGGAGTACGTGGCCACGATGAAGAAAAGTCAAAATAAAAACGGGTGATTGATTTATCAGCTAGGCTCTGAAAA GAGGAGTTTCCGTAATTTCCCCTTGAAGATGAAATATCGGTATCGGTAGTGATGTGAACATGAGAAAGACTATT CAAAGTCTCATTAAAAAATCCATCTTCCATAAAAATTTGAGTTAATTCACCCTCTAGAGATGACTTTTTAGTTT CTTGCAAATTCCCGGAATAAATACTCTGTTCTTCAGATGAAAGTTTATAAGGTGACTCTTTTAACAGAGTATTC AAAGCCGAAATGGCAAGTATTCTTGTTTGTGGAAGTTGACTTTTTAAATTTTTTAAGAAATGACCTGAAAAGGG AT GAAT GT CAATAT C CAT T GTAAT GAGAATAAAAAGATAAGAGGGAAAT TAGGAGGTAGACAATAT C GAAAC CA ACCCGCAGTTTCACTCAGAATTTTCGAAGAGGAACTCGGGTCATTTCTAGATCCCATTGCCAACAGGAGTAGGA CT CT GT TAGC CAT CAGAT T GTAC CTACAAAAAATAAAAAATAAAAATACATT TATAAC CAAGTAAAT GT T TAAG TATACATGAGAAAAAGTATCCAAGAAAATCAAGATATCTACCTCCAATGCAAACCAGTAGAATCGAAACTCATC GAAACAATCTGAGAAACCAAATCCCCAAAGTCTTGCCCCTCTAATTGATCGTTCGGTGCCCTGAAAATGTTTTT CGATACTCCAGAAAAGTGGATGTTGTACTTGACGAAAAGCTGCAGAAACTCGTTATATTAGATTCATTTCATTC AGAATTTCAGAATCAGAATCAACCTGAATTATCATACCTCATTTATTGCTTTCTGGGATTTCAGTGACTCGTGA TGAGAGCTTCAAGCAGATAAAAAGAAAGAAAAGTCAGATATCGTATGCACCCAAAACTACCGTTCAAATGTCAT TAGCAAACGATTACCTCGAGAGAATTCCAAGAATAAACGATGAGAATGCCTTCGGGTCCTGAAATTACAAAATA AGGTCAGAAATTTTTTCATTTCTTAGATATTATTTTATGTATAGCATGCAGTTTTTTTCGATCCTTACCGTTGT CACATGCTTGAGAACTGTTTGTGTCGCAAGGACTGCACAAGAACCTAGCACGGCATATTCTAATGAGCTGGAGT TTCGCAAGTTCTCAGTGAGAGTGAGAACACATTTTGAAATCATAGTAGGCCATCTCTTTATCATCTTCAAAAGC GATTTTCCAGCAAGCCTGAGTGTACCATGGCATTCAATCAAATTCGGTACTCAAAAATTCCAAAACCAGAACAA TTAATAATAAGCAAAGGCAATGGCTTTATTTAGGTTGCTATTTTTCCACTTCTGAAAGGCTAAAGAAGATTTGA AAACTTACGCCCGAACAGTTTCATAGCTGTGCAACGAAAGATTAAGAAGATCATCCATCAAAAGATTCACATGG TCCGATGGATAGAAATTTCCAGTAGTACGATATAAATGGTACGATGACTGTGAGGATCTCCATGTATTGTGCAT GTAAGCCTTATCAATAAGTGCCCACCTTGGCCTGGATTTTACAGAATAATTTAGTATCAATGCTAAGATAATAG AACTGGAGTTCCCGAAATCAAAAGGAAACTTCATATCATTACCTTCTTTTTCCCTTAGAATGGGATGACACAAT AAAATTAATCGGAGGTTCGATTATAGCAGCAGATTCTAACTTCCAAGCTTGTCTGTGATTTGCCCATTCATCGT ATTCCAAACTCCCTATAAGAGAGAAATATTAAAAAGGATGCATGTGTGTGAGGCAAATGTAAAAAACACGAAAC CATATAGATCACAGCCGTTACCATAGTTTGCCAAAGCATCCATGATACGAATAACAAGAATCAAAAGAATGCTG TCATCTGATTTTTCCTCTACTAAGTATCTGCTCAAAGCACAAAGAGCAACAAGCTTCAGTATCTCATATCGACT AAATAATAGAAAATCCATCATGAGATCTTTAACAAAATATTACTTGCAAGCTGTGTGTATAATGTCAGCAGCCT TTTCACGGAGAGCAATACTTCCAACAGTCGAACCAGTTGCTCCAGCTAATAAAAACGAGATATGCTCCTGATCT ACGACCATCTTATTTCTTGAAGATGGGATGAAATCTGGTAAACAAGACAAAACACCTTGCAAGGACGAATCTAT ACGCAAAAGAGTCACTTTTAAGTGCTCTTTCTCATCTCCTGGTTTCAAAAAAGTGAGTCAACTGCTGTGA Form Start End Extracted Seq-Start Extracted Seq-End Gene Name 5kbs upstream 372128 377127 1 5000 upstream regulatory region gene 377128 379260 5001 7133 gene biotype=protein coding 5kbs downstream 379261 384260 7134 12133 downstream regulatory region mRNA 377128 379260 5001 7133 gene=LOCl 15725196 exon 377128 377703 5001 5576 product=transcription factor MYB80 exon 378075 378217 5948 6090 product=transcription factor MYB80 exon 378304 378433 6177 6306 product=transcription factor MYB80 exon 378521 379260 6394 7133 product=transcription factor MYB80 CDS 377639 377703 5512 5576 gene=LOC 115725196 CDS 378075 378217 5948 6090 gcnc=LOC 115725196 CDS 378304 378433 6177 6306 gene=LOC 115725196 | CDS | 378521 | 379241 | 6394 | 7114 | gene=LOCl 15725196    | >MYB80 amino acid sequence from CBDRx (SEQ ID NO :17) 5 MGRIPCCEKENVKRGQWTPEEDNKLSSYIAQHGTRNWRLIPKNAGLQRCGKSCRLRWTNYLRPDLKHGQFSDAE EQTIVKLHSIVGNRWSIIAAQLPGRTDNDVKNHWNTKLKKKLSGMGIDPVTHKPFSHLMAEIATTLEPPQVAHL AEAALGCFKDEMLHLLTKRRIDFQLQQSNASIGNATTTYMNSKQEEHQDDEAIEKIKFGLSRSIQEPDMLPPNK LWDSATTGPTFSSFSGNCSSAFPYSGFQYEPSSFSNERDGSPWNQSICTGSTCMVGDRQSRLPDKLEENGDDSR TGKEIRNDAILFNTDSVLWDLPSDDLMHPIV 10 See APPENDIX H for the three most common genotype sequences in Cannabis accessions for MYB80. Table 8. List of Cannabis Accessions #a Accession Name #a Accession Name #a Accession Name 1 GCF 029168945.1 67 GRMa 133 FCSla 2 GCF 900626175.2 68 ITLvlb 134 H3S7b 3 GCF 900626175.1 69 MM3vlb 135 KCDvla 4 79X 70 S8Ha 136 OFP5 5 BOAXb 71 SKFBa 137 SDFBa 6 DPFBa 72 STLR 138 SNlv3b 7 FMvlb 73 TS13 139 SVA6a 8 161 74 WCFBb 140 TWFBb 9 MBFBa 75 YMv2b 141 WH94 10 PPFBb 76 AH3Mb 142 BH55 11 SHH24 77 CHMD3 143 COSVa 12 SSHS 78 EH23b 144 FCSlb 13 TK10 79 GRMb 145 HO40 14 USV 80 JL 146 KCDvlb 15 WIvlb 81 MNDB 147 P20 16 ABAC 82 S8Hb 148 SDFBb 17 CALO 83 SKFBb 149 SODLa 18 DPFBb 84 SUNDR 150 SVA6b 19 FOG 85 TST192 151 TWSVa 20 168 86 wcsv 152 WH96 21 MBFBb 87 BBKH 153 BLCvla 22 PTK32 88 CHMD5 154 COSVb 23 SHH5a 89 ERB 155 FIMvla 24 SSLR 90 GSC 156 13 25 TK7 91 JLD 157 KOMPa 26 W103 92 NLvla 158 P22 27 WLR 93 S8LR 159 SDI 28 ABC 94 SKSV 160 SODLb 29 CANN 95 SUV8 161 SZFBa 30 DPSVa 96 TST58 162 TWSVb 31 GERvla 97 WFvla 163 WHLR 32 175 98 BCMa 164 BLCvlb 33 MBSVa 99 CNBvla 165 CST53 34 PURD 100 FB191 166 FIMvlb 35 SHH5b 101 H3Sla 167 152 36 ssv 102 JLDSH 168 KOMPb 37 TKFBa 103 NLvlb 169 PBBK 38 W19LR 104 SAN2a 170 SG123 39 YMMvla 105 SKTZ 171 SSA1 40 ACBD 106 SV2 172 SZFBb 41 CBDRX 107 TURvla 173 UFBa 42 DPSVb 108 WFvlb 174 WHWa 43 GERvlb 109 BCMb 175 BLYS 44 178 110 CNBvlb 176 CST8 45 MBSVb 111 FB30 177 FIN 46 PURP 112 H3Slb 178 153 47 SHLR 113 JLF 179 LP10 48 STHa 114 OFBa 180 PK 49 TKFBb 115 SAN2b 181 SGVA 50 WC 116 SMSQ 182 SSA2 51 YMMvlb 117 SVA12a 183 TIBvla 52 ACDC 118 TURvlb 184 UFBb 53 CBIO2 119 WH106 185 WHWb 54 DRBP 120 BH4 186 BOAXa 55 GMO 121 COFBa 187 DCHCC 56 ITLvla 122 FBHO 188 FMvla 57 MM3vla 123 H3S7a 189 159 58 S8H2LR 124 JLM 190 LP6 59 SK88 125 OFBb 191 PPFBa 60 STHb 126 SCFB 192 SH50 61 TRWR 127 SNlv3a 193 SSH 62 WCFBa 128 SVA12b 194 TIBvlb 63 YMv2a 129 TWFBa 195 URKL 64 AH3Ma 130 WH72 196 WIvla 65 CHM9 131 BH44 197 LeafWorks cslO.l Annotation 66 EH23a 132 COFBb a, correspond to the numbers used in APPENDICES A-H. It is to be understood that, while the methods and compositions of matter have been described herein in conjunction with a number of different aspects, the foregoing description of the various aspects is intended to illustrate and not limit the scope of the methods and compositions of matter. Other aspects, advantages, and modifications are within the scope of the following claims. Disclosed are methods and compositions that can be used for, can be used in conjunction with, can be used in preparation for, or are products of the disclosed methods and 5 compositions. These and other materials are disclosed herein, and it is understood that combinations, subsets, interactions, groups, etc. of these methods and compositions are disclosed. That is, while specific reference to each various individual and collective combinations and permutations of these compositions and methods may not be explicitly disclosed, each is specifically contemplated and described herein. For example, if a 10 particular composition of matter or a particular method is disclosed and discussed and a number of compositions or methods are discussed, each and every combination and permutation of the compositions and the methods are specifically contemplated unless specifically indicated to the contrary. Likewise, any subset or combination of these is also specifically contemplated and disclosed. 15 Appendix A APPENDIX A SEQ ID NO:81 >122 ttacagaacatcgaatgttctcaaccacactgttacaagaagtcccagcagcagtaaaaagattagaaaaata atcagcaataatcacattcagactagaatcccaatccttctaaacaccattcgggtcttgtaatctcagaatt tggttacctcgtttcctagtgctggctgaggcatggaagtatttgctgtttttgtctcccgaatgaagccaat gttgcttggatctttgcttctagtaaagttcttgttgtgccaaaatctcaaaataattttttttttcttcatc aaattcagcagtagcaggatggattgttgaacttttcatggcagccattcgagttttgcttgtattcagcctg tgtttgaaattcccagtgagttcgaacccccaagctctcaaagtgttactgcaatgatgaattttaacagcta gagtagaattagcattaacctcccagcagtttttcacaatttgcttacaatatgactcacgactccatgcgtt ttcatatttaaaaaattgaatgtggttgcctcggttacttacagcaggttgcagaaaaatgggagtatggtca cttgaagaaaaatcacagttggataaaatggcattggggaaaagatcaagccaatgttgggttacaagagctt tatctaaccttatttcaacatgatgagtagtccctctccctcgttcccatgtgaaggcataaccgtgaagctt caaatcgacaaggcagcaatggcgaatgacatcttgaaagccattaatcaagtatgaggggtagggtcggcct ccttgtttttcttcttgagaagcaatgttattaaaatcgcctatgatacaccaaggcaaagtcgagcaagggg caatggtcttgagcaattgccaggtacggtcacgaagtgttcgatttggttcaccatagaagcccgtgagtct ccattgtgcagaaccaggagagtgaatttcaatgtcaatgtggttgtccaaatatccaagcagatgggcatca tcagtatttttccaaagaagagcaaggccacctttcctcccttgggcttcaacaacaaagcaactatcaaagc ccaacttagtttttatacgatccatagcatcttttttacacaaagtctcacataggaaaagaaaattgggttt cttttggacacaaatgtctgttaggaattgaatagcccgtgggttcccaagcccacgacaattccaactaaga gtattcatgatgagcggtgggcctgaacaccagagcccaccacaaaatcgttttttggcatctcctcagtatt tgcaggaataccaatatttatctcattaacttgttctatatcaaccatatttgccacatgtgaattcagtatg gaccccttctccccacgttttcttttaaggtccaatatggtaagttcatcatcagacataaaggtagaatcat tatcattaattacaattgatggaattttttctccattacccccattgactgaattgatatcaatatccctaac atcatgcatatagtcagctgacactgtattatcaaaataggcaaatttaccatgattggatactgaattgaat gccgccttgaattgtggagaagtattaactccatgatcactgtcttcaccgtcaacgacactaggctgagcag c ttg tttt gcag aacg aagccatg gaga tgcaaccaagaagttctggcgtctg gag g gage tttcatgtc gat gctatacggtttctcaatgcgatccgggtggagttcatacagttttttgcaaaaacgttcaaaatgtcccatt ataccacatataaagcagaaagtgggtactctttcatacttaaaattggcatagaatgtttcccctcttaatc ttttaaacttcattcgtcttttcaaagggagatcaatattcatcctaacacgaacccgtagaaactctcgcca tacacctttgaagttattgggatcggattcaagaaaagacccaatatattttccagcctgacgaacaacatgt tctgttttgaacccaggttgcagatcgtgtatctggacccataagtcaagatggttaatcggtttggttcgag gattatcacctggctttaatctctcaagaagcaactgtttgcgatcgtaagtccaaggactaccagtgatgac cctgtcaatatcaattteatggcagaattggaatagaaatoggttttgctcaagtatttttacatacatacct tttcctggtttccacaaatcagcaatgatattttgaaacacttgatagtctgagattttcctagaatggagtc tcccaataagacatcatccgtcatcgtactcaacctcatcagattcagcttcttctaactcagtaacaaactc ttcttcttcttcttctaaacagatattggctcattgtttctcaacatcattcataattctactgcttgaggcc atagtaataagacaaacactccaataagacaaacacaacactcaggaacaaagcttacagataaaaattctca taaacatacaaaactcccaatcacagagagacagcacacatgtcaaaagacatgacttatttcaaccacactc gtactcttctcctatctttaaataatattgagttgtgttttgtcaaacgatttgcaaataagctggctcatgt tttagccagggatttttgtttctcttcaggtcgtgtgcttcaattggagaattgttccaacgaagtgcgttct attgttattaacgaatgctagttaataaagttgctaagtttattaaaaaaaaaaatttaatttttaattgcag agatatttttataataaaaaatttaaagagtaaaaataaaaaaaaaataattatattacgtatttttgtaatt tta ttttt ttaa tttt tgg t caa t gtaaatttttctt tag taaat acta taacaaaggattaagtacagt tat tacatattgatataacaaaaatacaagtattacatctaatttgcaatttgttttccttacgaaagaaaaaaca tgtagtttctttctgaacaaaactacgcgttttagttggtggtcgctttcatacaaatattaatcgatgttga gctacttacaattacatctcaacaacaaactaaaaagaaaaaccattagtgggtgaccccttccaccacaacc tttgtctaaggttctttataaatttcttattttgttcttccttatgttcctttttttgttttaattgtttaca ctggaaacttatctaagaatatattttgcaaagaattatatctttggtttgaagtttcttccaccatcatttt gaacagtttcaattctattatagagctataaagttgagagtcttttatagttgcttagtgtgcattataattg ttcgagtaattattttataacgattaattctgtaaaataattatatgtataaaaaaaggttattttattttat gaatattaagtcaacaataagttaataacattttaaaaataaaccttcatttagtaaaagtcttggaaatggt ccaaatccggttgttggtagagtcataattcaaactgaatggttcaaaccaatataaaaggatttgggtagtt Appendix A tttttcgtttaaaggtatgtcatagaatacatttgatatacttttgggtccatcactctaaatgatgatttta ttaatttatccttacattttttgggaaacacgcttgtctgactgagcataactaataacataaaagccaattt attatcagtgatctgtaccacctgtttggaaaaagtcctctgtgtgagaatataacaaagctttttcagccca gcaaatttactttctgtgattgtctttcttcttcttcttctaattcacaaacaaagttcatcagaaaaccaat atttgtggtccttgaagaagctatgacaaaaagcaaaattttaaatcacttcactttgttgttgatccttata atctacacctggtttctaaagggagtaacctcagaagattacatagttggtgatggtgatgaatggaacagcc aaggcaactttttaacttggtcaacaaagtacaacttcactgttggtgatgtgcttggtatgatctttttgtt ttaaccctttaaatgttttattccttaaaaattatgtctgctatcatcttctaaaaacaagagtaaaagcaga agtcaatattgctttgacagagaaagtaaaatctaaccaaaccaattgctcctgaaaaaaacaactttgcttg gattggaaatcaaatgttttttttttcttgttccacctaagatattacatgttatgtagttttcaagtacgta aagggacaacacaatgcatatgaggtagatgagaaaacgtttcgatcatgtgatgcgagcagcggcgtgttga caaagtatgagagtggaaatgataaagtcaaactcactgaagccaagaagtattggtttatatgtaatgtatc aggccattgctttggtggaatgagatttagcattgatgttaaacaaggtaataatccctcttctgatgatacc aatccaaccacagatggttctccaacttctcaacctgatgagctgccagcagttccaccttccagctctagta gaagcactttgtccagtgacacttggaaaaatgggatttacactttggtatttgtgatcttattcaaattgtt ttattaatgaaatcagcaagttttttttttcttgtaattgttgtgcatatatagtaacattctttcagttcat aaaacaaccactgatctttagcttcccaagatttgtagacagtatggtgttggggaagttagtgtatgatggc tgaaagctatgtatttggtacatttatacatacacaacgcacatagaactttattggtatgtctgaaatcaat ttttgtgtaatgatcaagattattagcaaagagcacaagtggcacactaagcatttaaacatatagtgtgctt tttctatttctgtggaccaaacaaaaacagattctcagcatcaaatctgatacactttgctgagttacactag agtactaaatatatatgacatattgaaaattaatagattattcagtagaaatcttgcttccatttaaaacatt atgtttttaagcttgaagtgaattgagttcttatctcatttgaagaactgtaacttaattatgaaatcaataa gagggctggttgtgaagatggtgctgatagtcaatgctgctgetattatctgaaactttagacatcteagaca aacctctagaaggattccttgtcagctccagcaagaagtctctcaagtgatcagcttggactactattttact gtccttcttctgaatccaaaaattttaagcaacaaatagtagtttatatatgtagatgtgaaagatcttgcta agaaaaaaaaaaaaattgattagaagcattctacctctactttaagaacattggaaacaaggtttcgaaatgt agtaacgttggcattagttacttcaagagctagagaattcaaagcctccatcaatctcacaaatcttccaggc ttgtgctcacagaataccttcacaaagaactcatttccatctatttgagccacttccacttgcggctaaattt gtttcaaacatgttattatgaagcttaaaacacaaatatatcagaacgttaagaagagtagaagaacttatgt tacctccatctgtggtgccttgtcattagttgtatctgagtcttggttctgctttgagatgcttccactcctt gatgtttccaaatgaaattcattttgaactttttcatgctcaggcctagacctgaaattagtgtcgtcgcgac tcaaagtctcagacagaacattgtttcttttaccattaatgttggagtttaggccatcattgtctgattgctc ctctagctcatcttgaagctcttttgcctgactctgtaagtcttttacatactcaattgcatccccaagtatg gaggctctgtccaactgtggaacaaaaatgagatgaagaccttagattttacaactactagttccaattaaaa aaagcattaaatatctgttaccttagaaattttgggaaccaaagaccgaagagtatagagcctttcattaagc ttcttccttcttcttctctcagcaaaaaggttcttgcactgatctgattttcctgattttctcctatactttc cattateeteatcatcaatctgatcactacaatccgagactgaatttgatcttccttcttcctgtttgttcga gtccttttcattaccatgctgctccttgttttctgttacttgttccataaattgcatatgtgaattggttgtg teattcatcatggatggctgcatggcatccattgaagaatccaaatgcttatctggatgtaccgatggateat gagaacattcaggaaaggcagcatcgttcctggtcctactttcagaattgtaaccaaattgctgcaagctcat tggggaattgcatagacgagttcgatcaacagatacgtcataagacatgttcaggttctcaaatgctgttgaa tttggtgccggcatattattgttggaatccattttgtcagtttcattgcccaaaaatggccttgattggcttt cattcattacattaatgttgacacaaaagcttgtgtccatattgtttgtgttaatcagggcttcctgctccat caaaatgttgtattgagttgtgatgaagtctaccacattctgatcttctgatatctgtcaaaagaaactctat tataatacttacttggactgaagacaagagaaaatgattatatgaaaagacaaatactcatacctgtttattc acaaaaagctctaccaatgcaactggcaatggaatcaaaaccctggtcccaatagattcctgcaagttaatat agcttttagacaatggttgaaaattagagaagggtttagtttcaaaaacaacttacctcgagagcatgtgagc atgtgacagttgaaaagtttaaccagctgggttgattggagaccaaggtttgtgcataaattctgttcataac cttgtcttaagtataggtttaggattcataacataaataccagaaaaaaaaaatcatttatagtaaaaagcta aacaaaagaaatggttagtaccctgaatctgaaggcattgaagttggcaattgggcaagaagttcacaggaat taactcttgggtgttgacatatggtatccctgcatggaaggactggagaaactggaaaaatcagctcttcttc tcccccattttgtgtgttttcagtcecagcacaacagcateteatccactcaataaacotataacaaatceca aaaagtaacatgaaaataaacacacatacacgtatcatttaatttagttagtcatggacaatttctctaacct ttga t c tt cact tag t ttcc agag aaca caa taa tee catccttt egag ceca ctag age tctaaccctc tee Appendix A atcagatactgcattatcaggtccatattacctgcaaaataattgagaaaaatgcaaacacttcaaattctaa agaatccctttttctttttcttcttcttttctttattactaaaatgctaatcttattatgacactcacaagta ccaagagagatagcaatgccttgtgttgttctcaagataaatatgcttaacacttttgactgaatttaagtat tcatatgaaaaaacataagcagggtggaagaagcaaaactacaaggcagcagaaaacacaaaggaaaatctcc atacaaactgatcaggtgacaggaactgatatagttgcaccaagtttaagctaagacagctccccccacctca taccaaagtacccatctcatccaataagaaagaaactaaaataacaataacaataaaaataaaacaactttag acccttttataagtatttttttttttttttttgaggttgggagctacttatatcggtaggtgaactgcagcct aagcatgaaagaaggagaaattttcatatacaataactaatattattattatatctaacttttcttgttatgg aagattatataagcaaatattttaggaggaaagaattcattattaaaaagtatataaatatatattaagttag ttataatgaatgagagcatattgcatgacctaagagtgtggcgtttgtaaccttaactaccttgaagtttttc gctcgcttgggatttctatattcttacatgtttcaactactctataactttggactagaaatggaaaagtact atacgtaaacaaatttgtaaaagaaaatccagttgttcccaatatagatgtaaaaggaaaaatgacaagtctt ctccaacttgttggaaaagcctgaaaaagtataaactcgatctaatgtcgtttccttagaagtatgatgatgc ttttgattgattgtacatgaatgagtagtagtcctaagatcgagtagtatttgtttccctaaaggaataataa taatatatttatcgattgattttaaaaagatttagaggaaagaaattaactaattacacaaggaaatgagttg gtaatgatgagagaaggcttattgcatgacccaacttaaggcctacaaaacactaactaccatgctcaagttt atattaaattatattcatttcgttttggtttagtatattgcttcatgtttcaactactctatcacttattgta tcacatttgggaaacatacatcacacctttcatttagatagggaaatatattctggcctcattgtagtgattt tgttgtcacccagccatttagttgaaattttatttaatcaataagttatccatttttctctatgtcctacact ttgattgaatagtgatgtaagtcagtacaaaaagatgataatcttttcatgctttggagatattaacgttcga caagccaacattaaagctagacaacttgaattttcaaaatttggaagaaatgtcgatatctttggaaaatttg aaattatatgcctattatggactaaaattggtatcctaatctaaaactatacataatttgtaaaatgggataa cttttacttaaacccaattttaccccctcatttctaaactctctctctctctctctctctctctctctctctc tctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctagcgtt tttgaaatcgcactacccacgaatccccactcgaacgatgaacccaccgaactcaccacctcacggagggaac ccatccactgagtacgcgacgagcaaggtattttttgtttcccctttcagttttttaatttttttttcttatt gattatggtgaaaatgttgtttgttgatagatttaggttggatatgtgcattcttgattagatctagggttag atggagtagatctagggtctgtaatctttttgggcatgaaaatctgattttctagtttgatatgagcttgaag atgaaggcccgatgcatggcccgatgtaggcttgatgtagggctcgatggtccgatgcccattttggattttt ttttttaaattttttctatttataggactggcccgatgggcccgatggtcagatgcccactttaatttttttt tttctgtttataggactggctcgatggtgggcccgatgtcgcccgatgcttatgtaaaaaaaaaatagaattt gatatggcccgataggtccaatgatgggcccgatgcctgtgtaaaaaagtagaatttgatatggcccgatggg cccgatagtgggcccgatgggtgttaggatatttttagtattaaatttaggttaattttagcacatttttcgg ttaagtttaaattatgtttggagcagttttacgcttttatcttttaattttgcagatttaaaatagttgaaga gttggaaaatattaggagaaaaaggataaaatatcgcataaaaatatcttggggaaaagatcaaattcggatt cagaaaaaaaagataaaaacgtgtggaagttgggtgcagagaaccacacgggccgcggcatggtattagcatg ccatggcccgcctacatggaaaaaagttgaaggagcccctcacttggctacacgggccgcggcatgggtgtag catgccgcggcccgcctctttgtcaacagagaaaaaaatctggcgtgaggtttctcgaaaattcaaatctgaa tggaatttaaacatgtgcagttttccatctttttaggacctggatgcctatataaaaagcagaagagagttga tttcagtaagcaatttcagagagaaaaaaagtgagagttcagatacagagtagagaggtcaactgcaatactg gagacatactgctcagggttttcagcattcttcttttcttctcttctctatttttcatctcttttctatcagt taatatgtgtatttgtgcttcaatgaacataagtaactaaacactttattagggttagatggatattgtctaa cattgttttatggttttaatatgatttattttccccctaatttctatgtattctattttatttgtgcttaatt atttttaattgtctggccaccaattaactgtttatgatttcgatgcgagatctgagaagtgagtgtcaaatat gctatagtagaatagaactgaatttcgatataggacgagagtacatatatggtttagatagcttatagggttt ctgtatttaatgcctgttgcatgttaaatttatcacgagagtgtaaagtttacatgtaattgaggtttatatt atctgagaagactataaattaccttagtaaacctgctattgcataggaattaatattaggaaaataatcatat taggccatattcaataaaaacaattgaaatcgacaccctagtttattaactatcaattttctcgaatcgttgc ttccaactatttttcttatactttattattttttttcctatcttttatttaatcaaatagaagtaaaaattta attttaacgtacttaactacactccctgtgggatcgacctcactcctagtgagtctactacttgaaacaatac gtacacttgcgtgtgttaaaaagcgcaacaatggggcctgatggcttgacattgtagtgaaataaaagagaga agaagagagagggggtgatgtaaattggggggtattttagggatattataaaatgtgtcatattccacatata tcaaatattatgagtttagagaaatttttttaagcatatgtaagcataaaaaatcaaatttcccatatctttt tagacaaccaataaaatagataaatacatataatctcgaaaaattgactcaaaatgaccaaggaggacaatac Appendix A tttttgccgttaatgtatgatttataatggaaaatttgattatttatgcatactaataggagaaattaattcc ctaaacttatatatttcaaaattttaaaaataagtcaaatttacatttaactcaaaaatacccctctcatttt caaaccgcacttctttctctaacctctgttctctcccctctctctctctctctcgctcgatatcccttgaaca tcccaaccgaacccacacgaaccaccccacagacccacaaaatcgaacccatcgaaccccttcaacatcccca tcgacccacggcgctccccaccaacccacgtcgcacccccaccgacccacaatgcaccaccaccgacccacga tacccaaatgcacccccaaccgaattaaacacaatgtcaaaggtgagttcgtgttccgttttatagttttcag aatctcagatctgtgatttggacaaggtccgatggggcccgatagggtcgaatgaaactgtgagtttgcgagg taggagacgtaggtccgatgggggggggtccaatgggttcgattgtgtctgatgtggggtccgatgaggggca tccgattgtgtaaatggggggtattttaggaatatcataaaagttgtcatattctacaaatattagttattat aagtttaggggaaattttttaaccaaatgtaagcataaaaaatcaaatttccctttataattttatatgttat gtcgacaatgattgttgtggcagtttatggcgagtcacctatgatatgtgttaacgcagatttttgtcaacca aatttgagcctcacgatgataattcaactcagaaaaaaaaataaaatctatagaaaaataatatataaacaca gggttttttacgtggttttgcagttaaaattctacatagtccacgagtcaatcttatttacatttctgagtat tttttttttcagggcctctttcataagatttttttcgtccttattttgagaatgttagccactatttataatt gcatagtggcagttaattacaaagctgaccaaaatatgattacagtcattatccctaaaatcgtgggatctga ttacataaatatgtaaatgcggtgtatgatttttaaaatcatatagctgtgatttttccgcttttactgggtc aagaatatcgtgtattaaacacgtctttaattgttgaatctagagatgtctcgatagaaacttgattgtagtg atcccaatggcgagctggaaccattcttctttccgaggtcgataagatatttcttacgtcgatcgcgggtata cagaattcttctgacttgccagggttatgagectcgctectgttagtgggatgaaactataggaagtttcttc gtagcgagatggtcatctcgcattcttgttcttcgagccgatccgacttttacacttgatttgcttattgcaa gctaaggtatgtgttacctttctctggtgcttcgctattctccttcagcgacatatgatttctcgttaataca ctaagtgtcagttcgtacaagtattggtagtgtaacaattataacaagcatg SEQ ID NO:82 >88 gttacagaacatcgaatgttctcaaccacactgttacaagaagttccagcagcagtaaaaagattagaaaaat aatcagcaataatcacattcagactagaatcccaatccttctaaacaccattcgggtcttgtaatctcagaat ttggttacctcgtttcctagtgctggctgaggcatggaagtatttgctgtttttgtctcccgaatgaagccaa tgttgcttggatctttgcttccagtaaagttcttgctgtgccaaaatctcaaaataattgtttttttcttcat caaattcagcagtagcaggatggattgctgaacttttcatggcagccattcgagttttgcttgtcttcagcct gtgtttgaaattcccagtgagttcgaacccccaagctctcaaagtgttactgcaatgatgaattttaacaact agagtagaattagcattaacctcccatcagtttttcacaatttgcttacaatacggctcacgactccatgcgt tttgatatttaaaaaattgaatgtggttgcctcggttacttacaacaggttgcagaaaaatgggagtatggtc acttgaagaaaaatcatagttggataaaatggcattggggaaaagatcaagccaatgttgggttacaagagct ttatctaaccttatttcaacatgatgagtagtccctctccctcgttcccatgtgaaggcataaccgtgaagct tcaaatcgacaaggcagcaatggcgaatgacatcttgaaagccattaatcaagtatgaggggtagggttggcc tccttgtttttcatcttgagaagcaatgttattaaaatcgcctatgatacaccaaggcaaagtcaagcaaggg gcaatggtcttgagcagttgccaggtacggtcacgaagtgttcgatttggttcaccatagaagcctgtgagtc tccattgtgcagaaccagaagagtgaatttcaatgtcaatgtggttgtccgaatatccaagcagatgggcatc atcagtatttttccaaagaagagcaaggccacctttcctcccttgggcttcaacaacaaagcaactatcaaag cccaacttagtttttatacgatccctaacatcttttttacacaaagtctcacataggaaaaggaaattgggtt tcttctggacacaaatgtctgttaggaattgaatagcccgtgggttcccaagcccacgacaattccaactaag agtatteacgatgageggtgggcctgaacaccagagcccaccataaaattgttttttggcatctccteagtat ttgcaggaataccaatatttatctcattaacttgttctatatcaaccatatttgccacatgtgaattcagtat ggaccccttctccccacgttttgttttaaggtcctatatggtaagttcatcatcagacataaaggtagaatca ttaccattaattacaattgatggaattttttctccattacccccattgactgaattgatatcaatatccctaa catcatgcatatagtcagctagcactgtattatcaaaataggcagatttaccatgatcggatactgaattgaa tgccgccttgaattgtggagaagtattaactccacgatcactgtcttcaccgtcgacgacaccaggctgagca gcttgttttgcagaacgaagccatggagatgcaaccaagaagttctggcattttgagggagctttcatgtcga tgctatacggtttctcaatgcgatccggtgggagttcatacagttttttgcaaaaatgttcagaatgtcccat tataccacatataaagtagaaagtgggtactctttcatacttaaaattggcatagaatgtttcccctcttaat cttttaaacttcattcgtcttttcaaagggagatcaatattcatcctaacacgaacccgtagaaactctcgcc atacacctttgaagttattgggatcggattcaagaaaagacccaatatattttccagcctgacgaacaacatg ttctgttctgaacccaggttgcagatcgtgtatctggacccataagttaagatggttaatcggtttggtttga Appendix A ggattatcacctggctttaatctctcaagaagcaactgtttgcgatcgtaagtccaaggactaccagtgatga ccctgtcaatatcaatttcatggtagaattggaatagaaatcggttttgctcaagtatttttacatacatacc tttccctggtttccacaaatcagcaatgatattttgaaacacttgatagtctgagattttcccagaaaggagt ctcccaataagacatcatccgtcatcgtactcaacctcatcagattcattttcttctaactcagtaacaaact cttcttcttcttctaaatagatattggctcattgtttctcaacatcattcataattctactgcttgaggccat agtaataagacaaacactccaataagacaaacacaacactcaggaacaaagcttacagataaaaattctcata aacatacaaaactcccaatcacagagagacagcacacatgtcaaaagacatgacttatttcaatcacactcgt actcttctcttatctttaaataatattgagttgtgttttgtcaaacgatttgcaaataagctggctcatgttt tagccagggattcttgtttctcttcaggtcgtgtgcttcaattggagaattgttccaacgaagtgcattctat tgttattaacgagtgctagttaataaagttgctaagtttattcaaaaaaaaaaaattaatttttaattgcaga gatatttttataataaaaaatttaaagagtaaaaataaaaaaaaaataattatattacgtatttttgtaattt tttttttaatttttggtcaatgtaaatttttctttagtaaatactataaaaaaggattaagtacagttattac atattgatataacaaaaatacaagtattacatctaatttgcaatttgttttccttacgaaagaaaaaacatgt agtttctttctgaacaaaactacgcgttttagttggtggtcgctttcatacaaatgttaatcgatgttgagct acttacaattacatctcaacaacaaactaaaaagaaaaaccattagtgggtgaccccttccaccacaaccttt gtctaaggttctttataaatttcttattttgttcttccttatgttcctttttttgttttaattgtttacactg gaaacttatctaagaatatattttgcaaagaattatatctttggtttgaagtttcttccaccatcattttgta cagtttcaattctattatagagctataaagttgagagtcttttatagttgcttagtgtgcattataattgttc gagtaattattttataacgattaattctgtaaaataattatatgtataaaaaaatgttattttattttatgaa tattaagtcaacaataagttaataacattttaaacataaacctttatttagtaaaagtcttggaaatggtcca aatccggttgttggtagagtcataattcaaactgaatggttcaaaccaatataaaaggatttgggtagttttt ttcgtttaaaggtatgtcatagaatacattttgatatacttttgggtccatcactctaaatgatgattttatt aatttatccttacattttttgggaaacacgcttgtctgactgagcataactaataacataaaagccaatttat tatcagtgatctgtaccacctgtttggaaaaagtcctctgtgtgagaatataacaaagcattttcagcccagc aaatttactttttgtgattgtctttcttcttctaattcacaaacaaagttcatcagaaaaccaatatttctgg tccttgaagaagctatgacaaaaagcaaaattttaaatcacttcactttgttgttgatccttatagtctacac ctgttttctaaagggagtaacctcagaagaatacatagttggtgatggtgatgaatggaacagccaaggcaac tttttaacttggtcaacaaagtacaacttcactgttggtgatgtgcttggtatgatctttttgttttaaccct ttaaatgttttgttccttaaaaattatgtctgctatcatcttctaaaaacaagagtaaaagcagaagtcacta ttgctttgacagagaaagtaaaatctaactaaaccaaatgctcctcaaaaaaaaaaaaaaaaaacaactttgc ttggattggaaatcaaatgttttttttttttgttttgttttacctaagatattacatgttatgtagttttcaa gtacgtaaagggacaacacaatgcatatgaggtagatgagaaaacgtttcgatcatgtgatgcgagcagcggg gtgttgacaaagtatgagagtggaaatgataaagtcaaactcactgaagccaagaagtattggtttatatgta atgtatcaggccattgctttggtggaatgagatttagcattgatgttaaagaaggtaataatccctcttctga tgataccaatccaaccacagatggttctccaacttctcaacctgatgagctgccagcagttccaccttccagc tctagtagaagcactttgtccagtgacacttggaaaaatgggatttacactttggtatttgtgatcttattca aattgttttattaatgaaatcagcaagttttttttttcttgtaattgttgtgcatatatagtaacattctttc agttcataaaacaaccactgatctttagcttcccaagatttgtaggcagtatggtgttgggaagttagtgtat gatggctgaaagctatgtatttggtacatttatacatacacaacgcacatagaactttattggtatgtctgaa atcaatttttgtgtaatgatcaagattattagcaaagagcacaagtggcacactaagcatttaaacatataat gtgctttttctatttctgtggaccaaacaaaaacagattctcagcatcaaatctgatacactttgctgagtta cactagagtactaaatatatatgacatgttgaaaattaatagattattcagtagaaatcttgcttccatttaa aacattatgtttttaagcttgaagtgaattgagttcttatctcatttgaagtgaattgagttcttatgaaatc aataagagggctggttgtgaagatggtgctgatagtcaatgctgctgctattatctgaaactttagacatctc agacaaacctctagaaggattccttgtcagctccagcaagaagtctctcaagtgatcagcttggactactatt ttactgtccttcttctgaatccaaaaattttaagcaacaaatagtagtttatatatgtagatgtgaaagatct tgctaagaaaaaaaaaattgattagaagcattctacctctactttaagaacattggaaacaaggtttcgaaat gtagtaacgttggcattagttacttcaagagetagagaattcaaagcctccatcaatctcacaaatcttccag gcttgtgctcacagaataccttcacaaagaactcatttccatctatttgagccacttccacttgcggctaaat ttgtttcaaacatgttattatgaagcttaaaacacaaatatatcagaacgttaagaagagtagaagaacttat gttacctccatctgtggtgccttgteattagttgtatctgagtcttggttctgctttgagatgcttccactee ttgatgtttccaaatgaaattcattttgaactttttcatgctcaggcctagacctgaaattagtgtcgtcgcg actcaaagtctcagacagaacattgtttcttttaccattaatgttggagtttaggccatcatggtctgattgc tcctctagctcatcttgaagctcttttgcctgactctgtaagtcttttacatactcaattgcatccccaagta Appendix A tggaggctctgtccaactgtggaacaaaaatgagatgaagaccttagattttacaactactagttccaattaa aaaaagcattaaatatctgttaccttagaaattttgggaaccaaagaacgaagagtatagagcctttcattaa gcttcttccttcttcttctctcagcaaaaaggttcttgcactgatctgattttcctgattttctcctatactt tccattatcctcatcatcaatctgatcactacaatccgagactgaatttgatcttccttcttcctgtttgttc gagtccttttcattaccatgctgctccttgttttctgttacttgttccagaaattgcacatgtgtattggttg tgtcattcatcatggatggctgcatggcatccattgaagaatccaaatgcttatctggatgtaccgatggatc atgagaacattcaggaaaggcagcatcgttcctggtcctactttcagaattgtaaccaaattgctgcaagctc attggggaattgcatagacgagttcgatcaacagatacgtcataagacatgttcaggttctcaaatgctgtgg aatttggtgccggcagattattgttggaatccattttgtcagtttcattgcccaaaaatggccttgattggct ttcattcattacattaatgttgacacaaaagcttgtgtccatattgtttgtgttaatcagggcttcctgctcc atcaaaatattgtattgagttgtgatgaagtctaccacattctgatcttctgatatctgtcaaaagaaactct attataatacttacttggactgaagacaagagaaaatgattatatgaaaagacaaatactcatacctgtttat tcacaaaaagctctaccaatgcaactggcaatggaatcaaaaccctggtcccaatagattcctgcaagttaat atagcttttagacaatggttgaaaattagagaagggtttagtttcaaaaacaacttacctcgagagcatgtga gcatgtgacagttgaaaagtttaaccagctgggttgattggagaccaaggtttgtgcataaattctgttcata accttgtcttaagtataggtttaggattcataacataaataccagaaaaaaaaaatcatttatagtaaaaagc taaacaaaagaaatggttagtaccctgaatctgaaggcattgaagttggcaattgggcaagaagttcacagga attaactcttgggtgttgacatatggtatccctgcatggaaggactggagaaactggaaaaatcagctcttct tctcccccattttgtgtgttttcagtcccagcacaacagcatctcatccactcaataaacctataacaaatcc caaaaagtaacatgaaaatagacacacatacacgtatcatttaatttagttagtcatggacaatttctctaac ctttgatcttcacttagtttccagagaacacaataatcccatcctttcgagcccactagagctctaaccctct ccatcagatactgcattatcaggtccatattacctgcaaaataattgagaaaaatgcaaacacttcaaattct aaagaatccctttttctttttcttcttcttttctttattactaaaatgctaatcttattatgacactcacaag taccaagagagatagcaatgccttgtgttgttctcaagataaatatgcttaacacttttgactgaatttaagt attcatatgaaaaaacataagcagggtggaagaagcaaaactacaaggcagcagaaaacacaaaggaaaatct ccatacaaactgatcaggtgacaggaactgatatagttgcaccaagtttaagctaagacagctccccccacct cataccaaagtacccatctcatccaataagaaagaaactaaaataacaataacaataaaaataaaacaacttt agacccttttataagttttttttttttttttgaggttgggagetacttatatcggtaggtgaactgcageeta agcatgaaagaaggagaaattttcatatacaataactaatattattattatatctaacttttcttgttatgga agattatataagcaaatattttaggaggaaagaattcattataaaaaagtatataaatatatattaagttagt tataatgaatgagagcatattgcatgacctaagagtgtggcgtttgtaaccttaactaccttgaagtttttcg ctcgcttgggatttctatattcttacatgtttcaactactctataactttggactagaaatggaaaagtacta tacgtaaacaaatttgtaaaagaaaattcagttgttcccaacatagatgtaaaaggaaaaatgacaagtcttc tccaacttgctggaaaagcctgaaaaagtataaactcgatctaatgtcgtttccttagaagtatgatgatgct tttgattgattgtacatgaatgagtagtagtcctaagatcgagtagtatttgtttccctaaaggaataataat aatatatttatcgattgattttaaaaagatttagaggaaagaaattaactaattacacaaggaaatgagttgg taatgatgagagaaggcttattgcatgacccaacttaaggcctacaaaacactaactaccatgctcaagttta tattaaattatattcatttcgttttggtttagtatattgcttcatgtttcaactactctatcacttattgtat caca t ttg ggaa aca t aca t caca ccc t tea tttagatagggaaatatattctggcc tea ttg tag tga tttt gttgtcacccagccatttagttgaaattttatttaatcaataagttatccatttttctctatgtcctacactt tgattgaatagtgatgtaagtcagtacaaaaagatgataatcttttcatgctttggagatattaacgttcgac cagccaacattaaagctagacaactttaattttcaaaatttggaagaaatgtcgatatctttggaaaatttga aattatatgcctattatggactaaaattggtatcctaatctaaaactatacataatttgtaaaatgggataac ttttacttaaacccaattttaccccctcatttctaaactctctctctctctctctctctctctctctctctct ctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctc tctctctctagcgtttttgaaatcgcactacccacgaatccccactcgaacgatgaacccaccgaactcacca ccteaeggagggaacccatecactgagtacgcgacgagcaaggtattttttttttcccotttcagttttttaa tttttttttcttattgattatggtgaaaatgttgtttgttgatagatttaggttggatatgtgcattcttgat tagatctagggttagatggagtagatctagggtctgtaatctttttgggcatgaaaatctgattttctagttt gatatgagcttgaagatgaaggcccgatgcatggcccgatgtaggcttgatgtagggctcgatggtccgatgc cca 11 ttg ga tt tttt tttt aaaa tttt ttet a tttataggactggcccgatgggcccgatgtagg gee c gat ggtcagatgcccactttaatttttttttttctgtttataggactggctcgatggtgggcccgatgtcgcccga tgcttatgtaaaaaaaaatagaatttgatatggcccgataggtccaatgatgggcccgatgcctgtgtaaaaa agtagaatttgatatggcccgatgggcccgatagtgggcccgatgggtgttaggatatttttagtattaaatt Appendix A taggttaattttagcacatttttcggttaagtttaaattatgtttggagcagttttacgcttttatcttttaa ttttgcagatttaaaatagttgaagagttggaaaatattaggagaaaaaggataaaatatcgcataaaaatat cttggggaaaagatcaaattcggattcagaaaaaaaagataaaaacgtgtggaagttgggtgcagagaaccac acgggccgcggcatggtattagcatgccatggcccgcctacatggaaaaaagttgaaggagcccctcacttgg ctacacgggccgcggcatgggtgtagcatgccgcggcccgcctctttgtcaacagagaaaaaaatctggcgtg aggtttctcgaaaattcaaatctgaatggaatttaaacatgtgcggttttccatctttttaggacctggatgc ctatataaaaagcagaagagagttgatttcagtaagcaatttcagagagaaaaaaagtgagagttcagataca gagtagagaggtcaactgcaatactggagacatactgctcagggttttcagcattcttcttttcttctcttct ctatttttcatctcttttctatcagttaatatgtgtatttgtgcttcaatgaacataagtaactaaacacttt attagggttagatggatattgtctaacattgtattatggttttaatatgatttattttccccctaatttctat gtattctattttatttgtgcttaattatttttaattgtctggccaccaattaactgtttatgatttcgatgcg agatctgagaagtgagtgtcaaatatgctatagtagaatagaactgaatttcgatataggacgagagtacata tatggtttagatagcttatagggtttctgtatttaatgcctgttgcatgttaaatttatcacgagagtgtaaa gtttacatgtaattgaggtttatattatctgagaagactataaattaccttagtaaacctgctattgcatagg aattaatattaggaaaataatcatattaggccatattcaataaaaacaattgaaatcgacaccctagtttatt aactatcaattttctcgaatcgttgcttccaactatttttcttatactttattattttttttcctatctttta tttaateaaatagaagtaaaaatttaattttaacgtacttaactacactccctgtgggatcgacctcactcct agtgagtctactacttgaaacaatacgtacacttgcgtgtgttaaattgtaacgccccgatttcccgagacgt cacactagctagtccgtttaaaaccatttaagataaagacaataaaagacttctttaatgaaaaataactaag catgagatctcattatttttaaaacaaaacatttatttattcataaccttaaaatataaagctttacaaaaac tatttgaatcataatcttaaatgaaatatttttaaaccaaaacatcgtgacaatcccctaacatgtaatccac gcctcgagctcgccatcctcactgcttagttttgtctttacctgcacacagagtacccgtgagctaacgccca gtaagaagagctatgtaggacataacccccccaaccagataacatagtcataagtataacaatatcacaacat caaatcaattcataccatacttgcatacatatatcaacatatcatatcacatactattctcaaatacaatata acatcatatcacaatgtaatcttaatgcatgctatactcacacacataacacatagtatcttatcgcacattg tcctcacatacgataatctactcccactcatgttgatcagacatgaagtgtaacttgccctgccttgtgctgt tctcacactcggcatccaatagggcaatcccttatcacaagttgtactcacccatgataggataacctgccag taaacccatacaagcagccaaaaatatatcatgcagtgctatgctcacaccagcagcagaaaacctatcctat aagtgctatcctcacacaaacagtcaaaagtcttatcactatcacacactaataacattagcataaaataaca atctaccatagaataaaacatgtgaatacaaacccataatacagttgtatgtttcaacatacaccctgtgcac agatgtccactcttcttacctcagttgttaagaacaccttcttgctactccaagcacctcaataaattttctt gttgaggacaagatcctcaaatcccactgcttaagacttgttatctcgtcactactacctataacaacacatg gttcaaggccctaacattaaaattcgtactcttacagtacagcagaaagatcactatttttgactaacaaata tactaattcagtgaaagttgtcttcataaaaattatagaaaatttcattatctttccaatgatataaagatca ttaaaaatggattaaaactgaggaagttatgattgttttactgaaactatttctgagaaagaatatggagtaa cgaattacacaacttagcaaaaatacaaacttttgacattgaatggttcagaactagaagataacatcttcat caaagttttaggaaattaaattccctttccaatgataccaaaatctttgaaattggaattataatcaaagaga tattacaattttaccaaaacagttttgcaagaacaagattcaagaaacgaatcacatgatattgaataaaact aactttttgacatagtatgacttcgaattaacaaaaagtttcttcataaaacttatgggaaatcgaataagct ttccaacaatataaaaatctctaaaaacggatcaatatcaagagagatatcatcactttactgaaacttgttt ttctgaaaaacgaaaaaatataatggatccgaaccctagataacatttagcattattgagcaagaaaatattt catacctcttcacatatttctattcgatgtctcaagcccgcaagccttgattataaatccaaaactttaagaa tgaaggtagaaaataagaagaattgtggaaggtttgagaggagcaaacatg SEQ ID NO:83 >136 attgatggagaagccgaagaaaaaatggtgccaaaatatcgttcaatctcactaacaatgtcctcttccgtac tcaatctacggccatcctcagtcataatctctactattgcatttttcttcttcctacccgtcgctttattgtg aaaatatttagtattcctatcaccaagagccaaccaatccgcccgcgacctcaacttccagtaacactcctct cgagacaagagatcattaagtttagtctcaagacgctttacctcatccatacgaaccaatggggccgagacac taagaagateatccaactgtttctgtgtatetctcacaagtctaggaatcgacccaaacttacccttattcca cgcccccaactgatccgcacacctaccaaaaatatcaagaatacaatcttgactatccaacggtgacggaaca tctggagccaaccaagtccgatgaacaatgtcatgacagtcatcatctttcaaccaatgagtttcaaattgaa agcacctcttcctatcattctgctgagtctgaataacattctctagggaagccaccacaggttgatgatccga Appendix A atgaatcaagtcgccattgataactcgaaccgatggatacaaattatgccgatcctggttgcaaaaataacgg tcaagccgctcttgtacatgcgccataccttgccttttattcagccatgtaaagcattgtccttcaaacccca tattaaccaaggaacacttatccagagcttgttgaaaatcagacatagcagataaacttcgatcagcgcctcc tttcttctcattaatagaaagaatttcattaaaatcaccaccacatacccaaaggagggtaaaaagatcctta agccgacacaataatctccatgattcagatcgacaacttgccttaggatttccataaaagccagtgaacctcc acaaactcctccccggacattgcaccaaagcatcaatatgtccaaccgaatatgatttaacagacacctccca atcgtcattccacatcaacaacaaacctccactcttacccacacaatcaacatgaaaactatttggaaaatta acatgtcgccgaacaccttccgcccacccaccataaagtttagtttcagataagaaaactaaacttggggaaa acttacgaacaatcgttcgtaatgcagtcaacgcaccagggttccccaatccccgggcgttccaacaaagaag actcattgctcttggcgagcctgctccgcaggcctcgccaataaaatattctcatcctcaggaaccatgtcag attgttcctccaaacgtccctttttcacacatgtagaattaacttccatttccatcttccgtttcccacctcc accaaccttaggactcaccttatatttggcatttgcaacaatgacatgagacaacttagggctaataggaaaa gtggccgccttagacaccccattagggccacgaccctttgcaaaattgagacgcttccaagtcttcgatattt gtgcttgactaatcggttcaaatgtttgttggagtgcttggcgaaacgagageteatcttgattcgaatgaac aagaacacccttttccttttgatctttattgaacctttgctttcgatggtcactcaactctccctcaccatgc atgtccaccttcccactagttgaatcaacatecacacccgaagaatacacactacccgaccgcteettcccat tatcactatcgggcaacacaatgcccacagccgacacacaaacctgaggtgcagatgatggcaagagcacctc ttcgaacaccatatgatcaacattctgccttcccatctcagacctggtcacctccgactgaaacccaccagcc acaggaacattctcttcaatattggccttggaagccagttcttcatcacgttctcgggcctctaaaacagagg taggagccaactcctctggtgtagggccagaataacgaactcggttcctctcaacagccgaaacaatctttga agcttctcccatagtcataatcggcctgttagagggagaagtotetcgctttttctggtaccgagaccgatea cgtggcggagaac teg gage acac a tee acgaaccat acct ceca egg tcaaaattaggattaacc oca gcaa aatcccgcaaaggataatccaatgccttatgcccgatgattccacagtcaaaacaaaagtcaggtaaatgttc gtattgaaaaagaagtgaaacctcattaccttgatcatcgatcgccacacgtaagccacgcttaagaggctcc gtaatgttaattcgcaccctcactttcatggtgccattaacaatcttgatgtcttctagctttccaatcctct ccccccattctcttgcaaaaagttcagtcatacaagctaatgggacgttgttaatcgaaatccaaaaggatgt aaaatcaaagttcatttttgaaatttcacccaaccccactggtttcacaaaacaaatcaattgcttgtcaatt gtccatggaccaccaccatagaccctctgtcgatcctccctacagccaaagaaaaaacgaaacacattattgg aactcaattcttccacctgaaaccggtggtgaatcttccaaaccccattcatagccttctcgattccctcctt attggccggcctggggccgattaccttccccaccaaacatagatccatcttcttcttcccttcctcaatacca gccttattcaaggaaagaatagggccatcaccctcatcaagaaccaaaacctcagaaagtctattcaccaact ccaaatccattgaatgcacacagaaaatcgcagcaaaaccaagaaaaaacgaagcaaaaccaataaaaaagac gccggagatcgtcgactgcacccttcccatgaagggactgcgcttccctgttagtttcgtagctccgaaacta gtggaaggctaaaaagatgacactctatgaagtcaattatgcaatttgttttcctaattacgaaagaaaaaac atgtagtttctttctgaacaaaactacgcgttttagttggtggtcgctttcatacaaatattaatcgatgttg agctacttacaattacatctcaacaacaaactaaaaagaaaaaccattagtgggtgaccccttccactacaac ctttgtctaaggttttttataaatttcttattttgttcttccttatgttccttttttttgttttaattgttta cactggaaacttatctaagaatatattttgcaaagaattatatctttggtttgaagtttcttccaccatcatt ttgtacagtttcaattctattatagagetataaagttgagagtcttttatagttgcttagtgtgeattataat tgttcgagtaattattttataacgattaattctgtaaaataattatatgtataaaaaaatgttattttatttt atgaatattaagtcaacaataagttaataacattttaaacataaacctttatttagtaaaagtcttggaaatg gtccaaatccggttgttggtagagtcataattcaaactgaatggttcaaaccaatataaaaggatttgggtag tttttttcgtttaaaggtatgtcatagaatacattttgatatacttttgggtccatcactctaaatgatgatt ttattaatttatccttacattttttgggaaacacgcttgtctgactgagcataactaataacataaaagccaa tttattatcagtgatctgtaccacctgtttggaaaaagteetctgtgtgagaatataacaaagcattttcage ccagcaaatttactttttgtgattgtctttcttcttctaattcacaaacaaagttcatcagaaaaccaatatt tctggtccttgaagaagctatgacaaaaagcaaaattttaaatcacttcactttgttgttgatccttatagtc tacacctgttttctaaagggagtaacctcagaagaatacatagttggtgatggtgatgaatggaacagccaag gcaactttttaacttggtcaacaaagtacaacttcactgttggtgatgtgcttggtatgatctttttgtttta accctttaaatgttttgttccttaaaaattatgtctgctatcatcttctaaaaacaagagtaaaagcagaagt cactattgctttgacagagaaagtaaaatctaactaaaccaaatgcteetcaaaaaaaaaaaaaaaaaacaac tttgcttggattggaaatcaaatgttttttttttttgttttgttttacctaagatattacatgttatgtagtt ttcaagtacgtaaagggacaacacaatgcatatgaggtagatgagaaaacgtttcgatcatgtgatgcgagca gcggggtgttgacaaagtatgagagtggaaatgataaagtcaaactcactgaagccaagaagtattggtttat Appendix A atgtaatgtatcaggccattgctttggtggaatgagatttagcattgatgttaaagaaggtaataatccctct tctgatgataccaatccaaccacagatggttctccaacttctcaacctgatgagctgccagcagttccacctt ccagctctagtagaagcactttgtccagtgacacttggaaaaatgggatttacactttggtatttgtgatctt attcaaattgttttattaatgaaatcagcaagttttttttttcttgtaattgttgtgcatatatagtaacatt ctttcagttcataaaacaaccactgatctttagcttcccaagatttgtaggcagtatggtgttgggaagttag tgtatgatggctgaaagctatgtatttggtacatttatacatacacaacgcacatagaactttattggtatgt ctgaaatcaatttttgtgtaatgatcaagattattagcaaagagcacaagtggcacactaagcatttaaacat ataatgtgctttttctatttctgtggaccaaacaaaaacagattctcagcatcaaatctgatacactttgctg agttacactagagtactaaatatatatgacatgttgaaaattaatagattattcagtagaaatcttgcttcca tttaaaacattatgtttttaagcttgaagtgaattgagttcttatctcatttgaagtgaattgagttcttatg aaatcaataagagggctggttgtgaagatggtgctgatagtcaatgctgctgctattatctgaaactttagac atctcagacaaacctctagaaggattccttgtcagctccagcaagaagtctctcaagtgatcagcttggacta ctattttactgtccttcttctgaatccaaaaattttaagcaacaaatagtagtttatatatgtagatgtgaaa gatcttgctaagaaaaaaaaaattgattagaagcattctacctctactttaagaacattggaaacaaggtttc gaaatgtagtaacgttggcattagttacttcaagagctagagaattcaaagcctccatcaatctcacaaatct tccaggcttgtgctcacagaataccttcacaaagaactcatttccatctatttgagccacttccacttgcggc taaatttgtttcaaacatgttattatgaagcttaaaacacaaatatatcagaacgttaagaagagtagaagaa cttatgttacctccatctgtggtgccttgtcattagttgtatctgagtcttggttctgctttgagatgcttcc actccttgatgtttccaaatgaaattcattttgaactttttcatgctcaggcctagacctgaaattagtgtcg tcgcgactcaaagtctcagacagaacattgtttcttttaccattaatgttggagtttaggccatcatggtctg attgctcctctagctcatcttgaagctcttttgcctgactctgtaagtcttttacatactcaattgcatcccc aagtatggaggctctgtccaactgtggaacaaaaatgagatgaagaccttagattttacaactactagttcca attaaaaaaagcattaaatatctgttaccttagaaattttgggaaccaaagaacgaagagtatagagcctttc attaagcttcttccttcttcttctctcagcaaaaaggttcttgcactgatctgattttcctgattttctccta tactttccattatcctcatcatcaatctgatcactacaatccgagactgaatttgatcttccttcttcctgtt tgttcgagtccttttcattaccatgctgctccttgttttctgttacttgttccagaaattgcacatgtgtatt ggttgtgtcattcatcatggatggctgcatggcatccattgaagaatccaaatgcttatctggatgtaccgat ggatcatgagaacattcaggaaaggcagcatcgttcctggtcctactttcagaattgtaaccaaattgctgca agctcattggggaattgcatagacgagttcgatcaacagatacgtcataagacatgttcaggttctcaaatgc tgtggaatttggtgccggcagattattgttggaatccattttgtcagtttcattgcccaaaaatggccttgat tggctttcattcattacattaatgttgacacaaaagcttgtgtccatattgtttgtgttaatcagggcttcct gctccatcaaaatattgtattgagttgtgatgaagtctaccacattctgatcttctgatatctgtcaaaagaa actctattataatacttacttggactgaagacaagagaaaatgattatatgaaaagacaaatactcatacctg tttattcacaaaaagctctaccaatgcaactggcaatggaatcaaaaccctggtcccaatagattcctgcaag ttaatatagcttttagacaatggttgaaaattagagaagggtttagtttcaaaaacaacttacctcgagagca tgtgagcatgtgacagttgaaaagtttaaccagctgggttgattggagaccaaggtttgtgcataaattctgt tcataaccttgtcttaagtataggtttaggattcataacataaataccagaaaaaaaaaatcatttatagtaa aaagctaaacaaaagaaatggttagtaccctgaatctgaaggcattgaagttggcaattgggcaagaagttca caggaattaactcttgggtgttgacatatggtatccctgcatggaaggactggagaaactggaaaaatcagct cttcttctcccccattttgtgtgttttcagtcccagcacaacagcatctcatccactcaataaacctataaca aatcccaaaaagtaacatgaaaatagacacacatacacgtatcatttaatttagttagtcatggacaatttct ctaacctttgatcttcacttagtttccagagaacacaataatcccatcctttcgagcccactagagctctaac cctctccatcagatactgcattatcaggtccatattacctgcaaaataattgagaaaaatgcaaacacttcaa attctaaagaatccctttttctttttcttcttcttttctttattactaaaatgctaatcttattatgacactc acaagtaccaagagagatagcaatgccttgtgttgttctcaagataaatatgcttaacacttttgactgaatt taagtattcatatgaaaaaacataagcagggtggaagaagcaaaactacaaggcagcagaaaacacaaaggaa aatctccatacaaactgatcaggtgacaggaactgatatagttgcaccaagtttaagctaagacagctccccc cacctcataccaaagtacccatctcatccaataagaaagaaactaaaataacaataacaataaaaataaaaca actttagacccttttataagttttttttttttttttgaggttgggagctacttatatcggtaggtgaactgca gcctaagcatgaaagaaggagaaattttcatatacaataactaatattattattatatctaacttttcttgtt atggaagattatataagcaaatattttaggaggaaagaattcattataaaaaagtatataaatatatattaag ttagttataatgaatgagagcatattgcatgacctaagagtgtggcgtttgtaaccttaactaccttgaagtt tttcgctcgcttgggatttctatattcttacatgtttcaactactctataactttggactagaaatggaaaag tactatacgtaaacaaatttgtaaaagaaaattcagttgttcccaacatagatgtaaaaggaaaaatgacaag Appendix A tcttctccaacttgctggaaaagcctgaaaaagtataaactcgatctaatgtcgtttccttagaagtatgatg atgcttttgattgattgtacatgaatgagtagtagtcctaagatcgagtagtatttgtttccctaaaggaata ataataatatatttatcgattgattttaaaaagatttagaggaaagaaattaactaattacacaaggaaatga gttggtaatgatgagagaaggcttattgcatgacccaacttaaggcctacaaaacactaactaccatgctcaa gtttatattaaattatattcatttcgttttggtttagtatattgcttcatgtttcaactactctatcacttat tgtatcacatttgggaaacatacatcacacccttcatttagatagggaaatatattctggcctcattgtagtg attttgttgtcacccagccatttagttgaaattttatttaatcaataagttatccatttttctctatgtccta cactttgattgaatagtgatgtaagtcagtacaaaaagatgataatcttttcatgctttggagatattaacgt tcgaccagccaacattaaagctagacaactttaattttcaaaatttggaagaaatgtcgatatctttggaaaa tttgaaattatatgcctattatggactaaaattggtatcctaatctaaaactatacataatttgtaaaatggg ataacttttacttaaacccaattttaccccctcatttctaaactctctctctctctctctctctctctctctc tctctctctctctctctctctctctctctctctctctctctctctctctctagcgtttttgaaatcgcactac ccacgaatccccactcgaacgatgaacccaccgaactcaccacctcacggagggaacccatccactgagtacg cgacgagcaaggtattttttttttcccctttcagttttttaatttttttttcttattgattatggtgaaaatg ttgtttgttgatagatttaggttggatatgtgcattcttgattagatctagggttagatggagtagatctagg gtctgtaatctttttgggcatgaaaatctgattttctagtttgatatgagcttgaagatgaaggcccgatgca tggcccgatgtaggcttgatgtagggctcgatggtccgatgcccattttggattttttttttaaaattttttc tatttataggactggcccgatgggcccgatgtagggcccgatggtcagatgcccactttaatttttttttttc tgtttataggactggctcgatggtgggcccgatgtcgcccgatgcttatgtaaaaaaaaatagaatttgatat ggcccgataggtccaatgatgggcccgatgcctgtgtaaaaaagtagaatttgatatggcccgatgggcccga tagtgggcccgatgggtgttaggatatttttagtattaaatttaggttaattttagcacatttttcggttaag tttaaattatgtttggagcagttttacgcttttatcttttaattttgcagatttaaaatagttgaagagttgg aaaatattaggagaaaaaggataaaatatcgcataaaaatatcttggggaaaagatcaaattcggattcagaa aaaaaagataaaaacgtgtggaagttgggtgcagagaaccacacgggccgcggcatggtattagcatgccatg gcccgcctacatggaaaaaagttgaaggagcccctcacttggctacacgggccgcggcatgggtgtagcatgc cgcggcccgcctctttgtcaacagagaaaaaaatctggcgtgaggtttctcgaaaattcaaatctgaatggaa tttaaacatgtgeggttttccatetttttaggacetggatgcctatataaaaagcagaagagagttgatttea gtaagcaatttcagagagaaaaaaagtgagagttcagatacagagtagagaggtcaactgcaatactggagac atactgctcagggttttcagcattcttcttttcttctcttctctatttttcatctcttttctatcagttaata tgtgtatttgtgcttcaatgaacataagtaactaaacactttattagggttagatggatattgtctaacattg tattatggttttaatatgatttattttccccctaatttctatgtattctattttatttgtgcttaattatttt taattgtctggccaccaattaactgtttatgatttcgatgcgagatctgagaagtgagtgtcaaatatgctat agtagaatagaactgaatttcgatataggacgagagtacatatatggtttagatagcttatagggtttctgta tttaatgcctgttgcatgttaaatttatcacgagagtgtaaagtttacatgtaattgaggtttatattatctg agaagactataaattaccttagtaaacctgctattgcataggaattaatattaggaaaataatcatattaggc catattcaataaaaacaattgaaatcgacaccctagtttattaactatcaattttctcgaatcgttgcttcca actatttttcttatactttattattttttttcctatcttttatttaatcaaatagaagtaaaaatttaatttt aacgtacttaactacactccctgtgggatcgacctcactcctagtgagtctactacttgaaacaatacgtaca cttgcgtgtgttaaattgtaacgccccgatttcccgagacgtcacactagctagtccgtttaaaaccatttaa gataaagacaataaaagacttctttaatgaaaaataactaagcatgagatctcattatttttaaaacaaaaca tttatttattcataaccttaaaatataaagctttacaaaaactatttgaatcataatcttaaatgaaatattt ttaaaccaaaacatcgtgacaatcccctaacatgtaatccacgcctcgagctcgccatcctcactgcttagtt ttgtotttacctgcacacagagtacccgtgagctaacgcccagtaagaagagetatgtaggacataacccccc caaccaga...

Claims

1. A composition comprising an RNA nucleic acid molecule, wherein the RNA nucleic acid molecule is at least 10 nucleotides in length and has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to at least one of the genes selected from: AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80, wherein the RNA nucleic acid molecule effectively silences expression of the at least one gene, wherein the AMS gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLH9I gene comprises any of SEQ ID NOs: 2 or 89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYT1 gene comprises any of SEQ ID NOs: 5 or 109-115; wherein theMYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein the MYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124; wherein the MYB80 gene comprises any of SEQ ID NOs: 8 or 125-127.

2. The composition of claim 1, wherein the composition comprises RNA nucleic acid molecules at least 10 nucleotides in length and having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to at least two of the genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80, wherein the RNA nucleic acid molecules effectively silence expression of the at least two genes.

3. The composition of claim 1 or claim 2, wherein the composition is an aqueous solution.

4. The composition of any one of claims 1, 2, or 3, wherein the composition is formulated as a spray.

5. The composition of any one of claims 1, 2, or 3, wherein the composition is dried / lyophilized.

6. The composition of any of the preceding claims, wherein the RNA nucleic acid molecules are double-stranded (ds) RNA nucleic acid molecules.

7. The composition of claim 6, wherein the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs:33-43, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the AMS gene.

8. The composition of claim 6, wherein the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs:44-54, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the bHLH91 gene.

9. The composition of claim 6, wherein the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs:55-65, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the at TM6 gene.

10. The composition of claim 6, wherein the dsRNA nucleic acid molecules are selected from any of SEQ ID NOs:66-80, wherein each of the dsRNA nucleic acid molecules effectively silence expression of the STP6 gene.

11. The composition of claim 1, wherein the composition further comprises a delivery vehicle.

12. The composition of claim 11, wherein the delivery vehicle is a nanoparticle.

13. An article of manufacture, comprising:RNA nucleic acid molecules, wherein the RNA nucleic acid molecules are at least 10 nucleotides in length and have at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to at least one of the genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80, wherein the RNA nucleic acid molecules effectively silence expression of the at least one gene, wherein the AMS gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLPl91 gene comprises any of SEQ ID NOs: 2 or89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYT1 gene comprises any of SEQ ID NOs: 5 or 109-115; wherein the MYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein the MYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124;wherein the MYB80 gene comprises any of SEQ ID NOs: 8 or 125-127; and a delivery vehicle.

14. The article of manufacture of claim 13, wherein the delivery vehicle is a nanoparticle.

15. The article of manufacture of claim 14, wherein the nanoparticle is selected from chitosan salts (e.g., CQAS), carbon dots, liposomes, clay nanosheets, and gold nanoparticles.

16. The article of manufacture of any one of claims 13-15, wherein the delivery vehicle is conjugated to the RNA nucleic acid molecules.

17. The article of manufacture of any one of claims 13-15, wherein the delivery vehicle is not conjugated to the RNA nucleic acid molecules.

18. The article of manufacture of any one of claims 13-17, further comprising a linker.

19. The article of manufacture of any one of claims 13-18, wherein the article of manufacture is formulated as a spray.

20. A method of preventing hermaphroditism (e.g., in females) or the viability in male flowers (e.g., in male hermaphrodites or biological males) in Cannabis plants, comprisingcontacting Cannabis plants with the composition of any of claims 1-12.

21. The method of claim 20, wherein the contacting is spraying.

22. The method of claim 20 or 21, wherein the contacting is performed for about10 seconds to about 60 seconds (per plant) at least one time a day.

23. The method of claim 20 or 21, wherein the contacting is performed for about 10 seconds to about 60 seconds (per plant) a plurality of times each day.

24. A method of producing male sterile Cannabis plants (e.g., hermaphroditic female or a biological male), comprising:introducing a nucleic acid molecule into Cannabis cells to produce genetically-engineered Cannabis cells, wherein the nucleic acid molecule comprises a sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to one or more genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80 under direction of a promoter sequence, wherein the AMS gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLH91 gene comprises any of SEQ ID NOs: 2 or 89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYT1 gene comprises any of SEQ ID NOs: 5 or 109-115; wherein the MYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein the MYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124; wherein theMYB80 gene comprises any of SEQ ID NOs: 8 or 125-127; and regenerating a genetically engineered Cannabis plant from the genetically engineered Cannabis cells,wherein the genetically engineered Cannabis plant exhibits reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein.

25. The method of claim 24, wherein expression of the nucleic acid molecule triggers RNAi, thereby silencing expression of the at least one gene.

26. The method of claim 24 or claim 25, wherein pollen produced by the genetically engineered Cannabis plants is non-viable.

27. The method of any one of claims 24-26, further comprising selecting forgenetically engineered Cannabis plants.

28. A method of producing male sterile Cannabis plants (e.g., hermaphroditic female or a biological male), comprising:introducing a nucleic acid molecule into Cannabis cells under conditions in which the genome of the Cannabis cells is edited to produce edited Cannabis cells, wherein the nucleic acid molecule has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to one or more genes selected from AMS, bHLH91, TM6, STP6, DYTI, MYB35, MYB35, and MYB80, wherein the AMS’ gene comprises any of SEQ ID NOs: 1 or 81-88; wherein the bHLH91 gene comprises any of SEQ ID NOs: 2 or 89-92; wherein the TM6 gene comprises any of SEQ ID NOs: 3 or 93-99; wherein the STP6 gene comprises any of SEQ ID NOs: 4 or 100-108; wherein the DYT1 gene comprises any of SEQ ID NOs: 5 or 109-115; wherein the MYB35-15 gene comprises any of SEQ ID NOs: 6 or 116-119; wherein the MYB35-16 gene comprises any of SEQ ID NOs: 7 or 120-124; wherein the MYB80 gene comprises any of SEQ ID NOs: 8 or 125-127; andregenerating an edited Cannabis plant from the edited Cannabis cells, wherein the edited Cannabis plant exhibits reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein.

29. The method of claim 28, wherein the nucleic acid molecule comprises one ormore constructs comprising gene editing components for editing at least one of the genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80, wherein the gene editing reduces or knocks-out expression from the one or more genes or the expressed protein exhibits reduced, altered, or lack of function.

30. The method of claim 29, wherein the gene editing components are selectedfrom CRISPR / Cas components, TALEN components, or the like.

31. The method of any one of claims 28-30, wherein the pollen produced by the edited Cannabis plants is non-viable.

32. A method of making a Cannabis plant (e.g., hermaphroditic female or a biological male) male sterile, comprisingcontacting Cannabis seeds with a mutagen to create mutant Cannabis seeds;producing mutant Cannabis plants from the mutant Cannabis seeds; and screening the mutant Cannabis plants for those having a mutation in one or more genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80, wherein the mutant Cannabis plants exhibit reduced or no expression from the one or more genes or reduced, altered, or lack of expression or function of the expressed protein.

33. A method of evaluating a Cannabis plant for the propensity to trigger maleflower formation (e.g., hermaphroditism), comprising:determining the presence or absence of a variation in sequence within one or more genes selected from AMS, bHLH91, TM6, STP6, DYTJ, MYB35, MYB35, and MYB80, wherein the presence or absence of sequence variation within one or more of the genes is indicative of the propensity to trigger male flower formation (e.g., hermaphroditism) in the Cannabis plant.

34. A method of obtaining a male sterile or hermaphroditic-resistant Cannabis plant, comprising:a) crossing a first Cannabis plant and a second Cannabis plant, wherein the first Cannabis plant exhibits male sterility or hermaphroditic-resistance;b) hybridizing genomic DNA from cells obtained from the cross or from cells of later filial generations of the cross with one or more markers within one or more genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80 and;c) selecting a plant in which the genomic DNA hybridizes with one or more of the markers within one or more of the genes selected from AMS, bHLH91, TM6, STP6, DYT1, MYB35, MYB35, and MYB80 to obtain a male sterile or hermaphroditic-resistant plant.

Images