Reverse transcriptase variants

Abstract

The present invention provides MMLV reverse transcriptase enzymes with increased thermal stability as compared with wild type MMLV and AMV reverse transcriptases. The improved thermal stability allows for reverse transcription of RNA to cDNA at temperatures above 37 °C, thereby reducing error rates introduced during cDNA synthesis. As a result, the reverse transcriptases of the invention allow for increased accuracy in the determination of transcriptomes of living organisms. 1006737515 20 26 20 54 90 09 J ul 2 02 6 2 0 2 6 2 0 5 4 9 0 0 9 J u l 2 0 2 6 1 0 0 6 7 3 7 5 1 5

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