Rationally-designed single-chain meganucleases with non-palindromic recognition sequences

Abstract

Abstract: Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases. Abstract: 20 26 23 81 71 28 S ep 2 02 6 A b s t r a c t : 2 0 2 6 2 3 8 1 7 1 2 8 2 0 2 6 S e p

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