Compound and photodynamic therapy using specific chemical compounds for dermocosmetics

Description

1 / 26 “PHOTODYNAMIC COMPOUND AND THERAPY USING SPECIFIC CHEMICAL COMPOUNDS FOR DERMOCOSMETICS” Field of Invention

[001] The present invention relates to a process for photodynamic therapy, as its name suggests, employing its synergy with specific active ingredients or photosensitizing agents to enhance the action of other dermocosmetic compounds. History of the Invention

[002] Since primitive times, humankind has sought alternatives to combat the various illnesses that challenge life. The history of modern photodynamic therapy dates back to the attempts of ancient Egyptians to treat skin diseases with light-absorbable compounds. Documents dating back six thousand years report that the ancient Egyptians applied plant substances to produce photoreactions in tissues. In Egypt, India, and China, sunlight was used to treat skin diseases such as psoriasis, vitiligo, and cancer, as described in the Ebers Papyrus (WEISS et al., 2012) and the sacred Indian book Atharva Veda (WYSS, 2000). In ancient Greece, the Greek physician Herodotus defined the technique as Heliotherapy and emphasized the importance of exposure to sunlight for the restoration of health (ACKROYD et al., 2001).

[003] In 1900, the first scientific description of photodynamic therapy was reported, carried out by a medical student, Oscar Raab, and his professor, Herman Von Tappeiner, in Munich. These researchers were investigating the effect of acridine dye on paramecium cultures and observed that the interaction of this dye with light was lethal to the protozoa. During a Petition 870250017410, dated 05 / 03 / 2025, page 11 / 41 2 / 26 During a storm and exposure to numerous lightning strikes, there was a change in the ambient light conditions at the time of the experiments, leading the authors to postulate that the observed effect was caused by the transfer of light energy to the chemical substance acridine, a phenomenon similar to what occurs in plants through the absorption of light by chlorophyll. The photodynamic action was confirmed when it was observed that light and the dye, in isolation, did not show any effect on the protozoa (ACKROYD et al., 2001).

[004] In 1901, Niels Finsen published the first book focused on the treatment of diseases through light, entitled “Phototherapy,” and two years later, in 1903, the same researcher was awarded the Nobel Prize in Medicine for the use of eosin and light in the treatment of lupus vulgaris (PENG; MOAN; NESLAND, 1996; ALLISON et al., 2004). In 1901, Hereon Henrian Von Tappeiner discovered that oxygen was necessary for the development of light-mediated reactions. Thus, the term photodynamic therapy was created, known to this day, to describe this oxygen-dependent therapy (PENG; MOAN; NESLAND, 1996; ALLISON et al., 2004; BABILAS et al., 2005). Also in the same year, Von Tappeiner and Jesionek investigated the application of eosin and light to treat skin tumors (DOUGHERTY et al., 1978; KESSEL, 2004).Photodynamic therapy underwent a major advancement in 1908, when the first reports emerged on the use of porphyrins as photosensitizing substances. Sixteen years later, in 1924, Policard detected fluorescence in tumors exposed to porphyrin and subsequently irradiated with a Wood's lamp; a fact that enhanced the research carried out around the end of the 1940s. Petition 870250017410, dated 05 / 03 / 2025, page 12 / 41 3 / 26 when it was discovered that porphyrins preferentially accumulate in tumors (ACKROYD et al., 2001).

[005] In 1916, Albert Einstein postulated the first theory on lasers, "the principles of light by stimulated emission of radiation," which later formed the basis for the development of the first laser in 1960 by Theodore Maiman, triggering a series of research projects involving the interaction between light and tissue (FELDMAN, 2009). In the 1970s, Medicine gained a strong ally in the war against cancer when Thomas Dougherty initiated a series of clinical research projects involving hematoporphyrin derivatives, developing the formulation of these substances on a large scale, according to the standards established by the Food and Drug Administration (FDA) (KESSEL, 2004).

[006] From the same decade, reports of the effects of microbial resistance to antibiotics, observed years after the discovery of penicillin by Alexander Fleming in 1928, encouraged and triggered a series of research projects that continue to be carried out today, involving photodynamic therapy in the control of microorganisms. Photodynamic therapy, more commonly called PDT, is one of the most studied therapeutic modalities in the health field in recent decades. Its application involves the use of a photosensitizing agent, light, and molecular oxygen to destroy both neoplastic cells (ZEITOUNI; OSEROFF; SHIEH, 2003; MARMUR; SCHMULTS; GOLDBERG, 2004) and microorganisms (bacteria, viruses, and fungi). It does not present genotoxic or mutagenic effects and, therefore, does not allow the Petition 870250017410, dated 05 / 03 / 2025, page 13 / 41 4 / 26 development of microbial resistance (KONOPKA; GOSLINSKI, 2007).

[007] The photodynamic effect of PDT occurs when the photosensitizer molecule absorbs photons emitted by a light source with a specific wavelength resonant with the photosensitizer, transitioning from its ground state to an excited state. At this point, energy transformation can occur through fluorescence processes or internal conversion from the excited state to the ground state and / or a crossover process can occur between systems, leading the molecule to the triplet excited state. In this state, the photosensitizer molecules can interact with the medium through two distinct processes, called type I and type II mechanisms. In the type I mechanism, electron transfer reactions occur between the photosensitizer and the substrate, forming free radical species. In the type II mechanism, energy transfer occurs from the photosensitizer to oxygen in the ground state, resulting in the formation of singlet oxygen.The factors that will determine the selective death of host cells or microbial inactivation are directly related to the photosensitizing molecule used and the chemical and light dosimetries (ALLISON et al., 2004; HAMBLIN; HASAN, 2004). State of the Art

[008] PDT may be indicated in the treatment of a wide variety of cutaneous neoplasms in dogs and cats. In addition, its use has also been investigated in neoplasms located in the oral cavity, esophagus, prostate, and brain, in animals and humans. The application of PDT in malignant neoplasms of non-epithelial origin, such as glioma, retinoblastoma, liposarcoma, and Petition 870250017410, dated 05 / 03 / 2025, page 14 / 41 5 / 26 chondrosarcoma is also described in the literature (LUCROY, 2002; EMILIO, 2008). Although studies involving animals have been reported since the 1980s, therapeutic protocols in veterinary medicine are still widely debated due to the many variables involved in this type of procedure and the limited number of studies in the literature compared to other health fields such as Medicine, Physiotherapy, and Dentistry.

[009] Photonics is the branch of science and engineering that studies the interaction of light with matter. Light is a form of electromagnetic radiation that propagates in waves or beams of particles called photons, and can assume various colors, depending on the frequency or wavelength of the electromagnetic wave, from red to violet, within the visible spectrum, or infrared and ultraviolet, outside this spectrum.

[010] Some chemical components have the property of absorbing, reflecting, emitting, or modifying the light that falls on them, according to their molecular structure and their physical or chemical state. These components can be used to create photonic devices, which manipulate light for various purposes, such as communication, computing, sensing, lighting, or medicine. They are known as photosensitizing agents.

[011] An example of the application of photonics in medicine is the use of light beams to activate other components chemically bound to them or to allow better absorption by the skin. For example, some therapeutic agents can be conjugated with photosensitive molecules, which are activated when exposed to a certain color of light. This technique, called photodynamic therapy, can be used to treat certain types of cancer, infections, or diseases. Petition 870250017410, dated 05 / 03 / 2025, page 15 / 41 6 / 26 inflammatory, in a selective and minimally invasive way. Another example is the use of low-intensity lasers to stimulate wound healing, reduce pain, or increase blood circulation by increasing cell permeability and collagen production. It is precisely in stimulating wound healing and cell permeability and collagen production that this invention proposes to contribute.

[012] Photodynamic therapy (PDT) is a health treatment that consists of applying a photosensitizing substance to the skin, followed by irradiation with a light source appropriate to its absorption spectrum. PDT can be used to treat various skin diseases, such as acne, psoriasis, vitiligo, among others. PDT has the advantages of selectivity, preservation of adjacent normal tissue, absence of bacterial resistance, possibility of repeating the treatment, and good aesthetic and functional results. In addition, PDT can also have beneficial effects on skin aesthetics, as it increases the permeability of epidermal and dermal cells, allowing greater penetration of dermocosmetics. PDT also stimulates collagen production, improving skin firmness and elasticity, promoting wound healing, and reducing marks and scars.

[013] Currently there are some therapeutic processes that compete along the same lines as photodynamic therapy (PDT), including: 1- The fractional CO2 laser, which uses infrared light beams to create small thermal zones in the skin, causing micro-lesions and stimulating the production of collagen and elastin. The CO2 laser Petition 870250017410, dated 05 / 03 / 2025, page 16 / 41 Fractional 7 / 26 is used to treat wrinkles, scars, blemishes, stretch marks, and sagging skin; 2- Microdermabrasion, which consists of the mechanical removal of the superficial layers of the skin, using a device that exfoliates the skin with aluminum oxide crystals or a diamond tip. Microdermabrasion is used to improve skin texture, radiance, and evenness, as well as reduce enlarged pores, blackheads, fine lines, and superficial blemishes; 3- Mesotherapy, which involves injecting substances such as vitamins, minerals, amino acids, enzymes, and hyaluronic acid into the dermis or subcutaneous tissue, aiming to nourish, hydrate, rejuvenate, and remodel the skin. Mesotherapy is used to treat cellulite, localized fat, sagging skin, wrinkles, dark circles, and alopecia. One of the advantages of this method is the use of pressurized guns, with a small amount of the dermocosmetic product along with the photosensitizing substance, achieving the same result (making the dermocosmetic product penetrate the deeper layers of the skin) without the need for an invasive procedure or the use of local anesthetics.

[014] One of the drawbacks observed in the approaches listed in the state of the art concerns their limited effectiveness and treatment time, since all procedures act in isolation, without the combination of a photosensitizing agent with a light source, capable of activating the therapeutic effect of a dermocosmetic product.

[015] Current products have limited absorption by the skin, and may remain trapped in the epidermis or stratum corneum, without reaching the deeper layers where changes occur. Petition 870250017410, dated 05 / 03 / 2025, p. 17 / 41 8 / 26 aesthetic or lesional. They can cause irritation, sensitization, redness, peeling, or allergic reactions on the skin, especially if used in high concentrations or for long periods. They may also have a nonspecific action, affecting normal cells or healthy tissue as well, causing damage or unwanted effects such as blemishes, scars, atrophy, or necrosis. They may also have insufficient action, failing to completely eliminate abnormal or damaged cells, such as inflammatory cells or resistant bacteria. The use of fractional laser

[016] Fractional laser is an advanced technology used in aesthetic and therapeutic treatments, which creates microzones of thermal lesions in the skin. These areas are surrounded by healthy tissue, allowing for faster and safer healing. This technology is highly effective in promoting skin regeneration, providing greater absorption of dermocosmetics, especially when combined with the appropriate photosensitizer.

[017] There are several types of fractional lasers, each with its own specific characteristics and applications. Among the most commonly used are: • CO2 Laser: Uses infrared light beams with a wavelength of around 10,600 nm. It is highly effective in facial rejuvenation, acne scars, and other conditions that require deep skin penetration. • Erbium:YAG Laser: Operates at a wavelength of 2,940 nm, ideal for removing superficial wrinkles and Petition 870250017410, dated 05 / 03 / 2025, page 18 / 41 9 / 26 skin rejuvenation, with less thermal damage compared to CO2. • Nd:YAG Laser: With a wavelength of 1,064 nm, it is frequently used to treat vascular and pigmentary lesions, as well as for skin rejuvenation. • Thulium Laser: Works at 1,927 nm, being effective in the treatment of pigmentation and superficial rejuvenation. Light strips and photosensitizers

[018] The operation of fractional laser is based on the delivery of highly concentrated light beams that penetrate the skin, creating small thermal zones. These heat zones stimulate cell regeneration and the production of collagen and elastin, improvements that result in firmer and rejuvenated skin.

[019] The therapeutic effect and absorption of dermocosmetics are optimized when using a fractional laser or a specific LED color in combination with the correct photosensitizer. This combination works similarly to a lock-and-key chemical reaction, where the precise match between the photosensitizer and the laser wavelength is crucial for treatment effectiveness. Without this correct combination, dermocosmetics may not be adequately absorbed by the skin. LED lights and their therapeutic effects

[020] In addition to fractional lasers, light generated by LEDs also has significant therapeutic properties. Each LED color corresponds to a specific wavelength, which can be used to treat different skin conditions: Petition 870250017410, dated 05 / 03 / 2025, page 19 / 41 10 / 26 • Blue Light: Wavelength of approximately 415 nm, effective in treating acne and reducing bacteria on the skin. • Green Light: Wavelength around 525 nm, used to reduce pigmentation spots and even out skin tone. • Red Light: Wavelength of approximately 630 nm, promotes healing, stimulates collagen and elastin production, and improves blood circulation. • Yellow Light: Wavelength of approximately 590 nm, used to reduce redness and inflammation, as well as improve skin texture.

[021] Like fractional lasers, LEDs create specific effects on the skin that can be enhanced by combining them with appropriate photosensitizers, improving the absorption of dermocosmetics and the effectiveness of treatments.

[022] The use of fractional lasers and LED lights in photodynamic therapy and aesthetics offers numerous advantages, from cell regeneration to improved absorption of dermocosmetic products. The appropriate combination of light technology and photosensitizers is essential to maximize therapeutic benefits, promoting more effective and safe treatments. Duration and Frequency of Photodynamic Therapy

[023] The specific duration of photodynamic therapy application and the frequency of sessions vary according to the type of treatment, the wavelength of the laser used, and the condition being treated. The duration of light application combined with dermocosmetics and their respective photosensitizer depends on several factors, including the type of laser, the condition of the skin or tissue being treated. Petition 870250017410, dated 05 / 03 / 2025, page 20 / 41 11 / 26 and the presence of bacteria at the site, therefore a duration range is provided below, as there is individuality in each case.

[024] We must also remember that the duration and frequency of laser use may vary depending on skin type. This is due to differences in the amount of melanin, the inflammatory response, and the overall sensitivity of light skin compared to darker skin.

[025] For light skin, laser treatment can be used with greater intensity and frequency, as the lower amount of melanin reduces the risk of post-inflammatory hyperpigmentation. However, it is crucial to monitor the skin to avoid adverse effects such as excessive redness or burns.

[026] For darker skin tones, the approach should be more cautious. The higher amount of melanin can increase the risk of hyperpigmentation and scarring. Therefore, the laser intensity should be reduced and the frequency of sessions may need to be adjusted, often starting with fewer sessions per week and gradually increasing as the skin's response is assessed.

[027] Regardless of skin type, a detailed patient assessment and personalization of the treatment protocol are essential to ensure safety and efficacy, as well as knowing the patient's history of allergic reactions to certain components that may be in the dermocosmetic formulation.

[028] Below are some average parameters, based on the efficacy detected in the treatment of individuals with white skin. As already explained, the frequency of the light wave should be reduced (around 10% to 15% of the cited value) and the frequency of sessions. Petition 870250017410, dated 05 / 03 / 2025, p. 21 / 41 The 12 / 26 weekly dose should be the lower dose for individuals with darker skin. For individuals of Asian descent, the same standards as for Caucasian skin can be used. • Acne: The use of blue light (415 nm) generally requires sessions of 15 to 30 minutes, 2 to 3 times a week. Application helps reduce bacteria and inflammation on the skin, and the use of a specific photosensitizer (see table) allows for faster and more effective treatment. • Pigment Spots: Green light (525 nm) is used for approximately 15 to 20 minutes per session. These treatments are usually performed 1 to 2 times per week, depending on the severity of the spots. • Skin Rejuvenation: Red light (630 nm) is applied for 20 to 30 minutes per session. For significant results, 1 to 2 sessions per week are recommended. • Reduction of Redness and Inflammation: Yellow light (590 nm) requires sessions of 15 to 25 minutes, twice a week for best results. • Wound Healing: To promote healing, red light is applied for approximately 20 to 30 minutes, 2 to 3 times per week. • Muscle Injuries: Laser treatment for muscle injuries typically lasts 15 to 25 minutes, with sessions 2 to 3 times per week, using red light to stimulate tissue regeneration. • Stretch Mark Treatment: Photodynamic therapy for stretch marks generally involves the use of red light (630 nm) and, in some cases, infrared light, depending on the interaction with... Petition 870250017410, dated 05 / 03 / 2025, p. 22 / 41 13 / 26 the photosensitizer. Each session usually lasts 20 to 30 minutes, with 2 to 3 sessions per week recommended. • Aseptic Healing: For aseptic healing (clean wounds without infection), red light (630 nm) combined with a dermocosmetic is frequently used due to its ability to promote tissue regeneration and reduce inflammation. Sessions should last between 20 and 30 minutes, with a frequency of 2 to 3 times per week. • Healing of Diabetic Ulcers: Red light (630 nm) and infrared light are frequently used to treat diabetic ulcers due to their ability to stimulate blood circulation and promote healing. Hence the importance of using the appropriate dermocosmetic, along with its photosensitizer, which will be activated within these ranges. For more information on this, see the table. Sessions last 20 to 30 minutes, with a frequency of 3 times a week.

[029] The frequency of laser therapy sessions also varies based on the condition being treated and the treatment goals. Below are some general recommendations: • Aesthetics and Skin Rejuvenation: For aesthetic and rejuvenation treatments, 1 to 2 sessions per week are generally recommended. A complete course can last from 4 to 8 weeks, depending on the individual skin response. • Wound Healing and Acne: For wound healing and acne treatment, the recommended frequency is 2 to 3 sessions per week, with an average duration of 4 to 6 weeks, or until the desired results are achieved. Petition 870250017410, dated 05 / 03 / 2025, page 23 / 41 14 / 26 • Reduction of Spots, Stretch Marks and Pigmentation: Treatments for pigmentation spots can be performed 1 to 2 times per week, with the duration of treatment varying from 6 to 8 weeks. • Stretch Mark Treatment: The total course of treatment can vary from 8 to 12 weeks, depending on the individual patient's response and the severity of the stretch marks. • Aseptic Healing: The total duration of treatment can be 4 to 8 weeks, until healing is complete. • Healing of Diabetic Ulcers: Due to the complexity of diabetic ulcers, treatment can last from 8 to 16 weeks, depending on the patient's response and the severity of the ulcers. • Muscle Injuries and Pain: To treat muscle injuries and relieve pain, 2 to 3 weekly sessions are generally recommended, with the duration of treatment depending on the severity of the injury. Sessions may continue until the pain is substantially reduced or the injury is healed.

[030] The following are some examples of commonly used Treatment Protocols: • Acne: A patient with moderate acne may be recommended for 3 weekly sessions of 20 minutes each with blue light, for a period of 6 weeks. • Skin Rejuvenation: A patient seeking facial rejuvenation may receive 2 weekly 30-minute sessions with red light for 8 weeks. Petition 870250017410, dated 05 / 03 / 2025, page 24 / 41 15 / 26 • Pigmentary Spots: For an individual with dark spots, 2 weekly sessions of 20 minutes with green light may be recommended for 6 weeks. • Muscle Injuries: An athlete with a muscle injury can be treated with 3 weekly sessions of 25 minutes using red light, continuing until full recovery.

[031] Therefore, the duration and frequency of photodynamic therapy sessions plus the use of dermocosmetics with the addition of photosensitizers should be adjusted according to the specific condition to be treated and the patient's response to treatment. The application of dermocosmetics and photosensitizers can be carried out by using the substance in the form of cream, ointment, gel or even through the use of a pressurized gun.

[032] It is also necessary to remember that conditions such as autoimmune diseases (psoriasis, for example) or diabetes (in cases of healing, but even in other situations) will have a longer treatment time than in patients without these conditions, usually adding 2 to 4 weeks more in total.

[033] In addition to using dermocosmetics before photodynamic therapy sessions (laser or LED), these same products can also be used at home, up to 3 times a day, even on days when light will not be applied. In these cases, the photosensitizer will not have its expected effect (greater penetration into the epidermis and action of the dermocosmetic in the dermis), but it will also not impair the use of the active ingredient.

[034] If the patient does not wish to use the product at home, they should use a regular moisturizer at least twice a day, except in cases of occlusion when the wound is healing, as well as in cases of injury. Petition 870250017410, dated 05 / 03 / 2025, page 25 / 41 16 / 26 muscular, where the use of the dermocosmetic should be maintained at least twice a day.

[035] The time observed for initial clinical effects in different photodynamic therapy treatments with the addition of photosensitizer-activated dermocosmetics varied depending on the nature of the treatment, as listed below: • Anti-aging Treatment (Wrinkles and Expression Lines): Photodynamic therapy with the addition of photosensitizing-activated dermocosmetics for anti-aging generally begins to show the first signs of improvement in wrinkles and expression lines in approximately 4 to 6 weeks of continuous treatment. The skin may appear firmer and smoother, with a more even texture. • Acne Treatment: In the case of acne, initial clinical effects, such as reduced inflammation and decreased acne lesions, can be observed in about 2 to 4 weeks. The skin tends to show less redness and a significant reduction in acne breakouts. • Aseptic Healing: For aseptic healing, initial effects can be noticed in 1 to 2 weeks. Photonic therapy helps accelerate the tissue regeneration process, minimizing scar formation and promoting faster recovery. • Diabetic Ulcer Healing: In cases of diabetic ulcers, the first signs of improvement may appear in about 3 to 4 weeks. Photonic therapy with the addition of Petition 870250017410, dated 05 / 03 / 2025, page 26 / 41 17 / 26 dermocosmetics activated by photosensitizers contribute to the reduction of inflammation and acceleration of healing, helping to improve the condition of the ulcer and prevent infections. • Skin Lightening Treatment: For skin lightening, initial clinical effects generally begin to be visible in approximately 4 to 6 weeks of continuous treatment with photodynamic therapy and dermocosmetics activated by photosensitizers. During this period, patients may notice a reduction in dark spots and greater uniformity in skin tone. • Stretch Mark Treatment: In combating stretch marks, the first signs of improvement can be observed in about 6 to 8 weeks. Photodynamic therapy, in combination with photoactivated dermocosmetics, helps to increase the production of collagen and elastin, which contributes to reducing the appearance of stretch marks and improving skin texture. • Pain or Inflammation Following Minor Muscle Injury (muscle strain): For pain or inflammation resulting from minor muscle injuries, initial clinical effects, such as pain and inflammation reduction, may be noticed within 1 to 2 weeks. Photodynamic therapy with the addition of photosensitizing-activated dermocosmetics helps reduce discomfort and promote muscle recovery.

[036] The time it takes for clinical effects to begin to appear may vary depending on the severity of the condition, the individual response to treatment, and the consistency in the continuity of therapies. It Petition 870250017410, dated 05 / 03 / 2025, page 27 / 41 18 / 26 It is always essential to follow the guidelines below for the best possible result. Home care during photodynamic therapy treatment in combination with photoactivated dermocosmetics:

[037] Patients undergoing photodynamic therapy and using dermocosmetics should follow certain recommendations to ensure the effectiveness of the treatment and avoid possible complications. Below are some detailed guidelines on what to avoid and how to proceed during and after photodynamic therapy sessions in combination with dermocosmetics and photosensitizing agents:

[038] Avoiding direct sun exposure is crucial, especially after photodynamic therapy sessions in combination with dermocosmetics. The skin may be more sensitive and susceptible to damage caused by UV rays. Therefore, it is recommended: • Use sunscreen with a high sun protection factor (SPF 30 or higher) every day, even on cloudy days. • Apply sunscreen at least 30 minutes before going outside and reapply it every 2 hours, or after excessive sweating. • Wear wide-brimmed hats and clothing that covers most of your skin when exposed to the sun.

[039] Avoid swimming pools and salt water for at least 48 hours after each photodynamic therapy session. Chlorine and salt can irritate the treated skin and possibly interfere with the effectiveness of dermocosmetics. However, normal bathing can be done with some precautions: • Use lukewarm water, avoiding temperatures that are too hot or too cold. Petition 870250017410, dated 05 / 03 / 2025, page 28 / 41 19 / 26 • Prefer mild or neutral soaps to wash treated skin. • Avoid rubbing the skin vigorously; dry it gently with a towel.

[040] It is permitted to use shampoo and conditioner normally, but make sure that the products do not drip or come into prolonged contact with the treated area.

[041] There are no specific dietary restrictions directly related to photodynamic therapy in combination with dermocosmetics and photosensitizing agents. However, a balanced and healthy diet can promote recovery and overall skin health.

[042] Some additional precautions are also recommended: • Continue using the dermocosmetics recommended by the specialist up to 3 times a day, even on days without light application; • If the patient chooses not to use the dermocosmetics used in photodynamic therapy at home, they should at least use a regular moisturizer twice a day. Keeping the skin well hydrated is essential; • For muscle injuries or healing, it is important to continue using the dermocosmetic product used in photodynamic therapy at least twice a day. • Avoid intense physical activities that may cause excessive sweating in the first 24 hours after the session. • After this period, physical activities can be gradually resumed, as directed by a specialist; • Avoid using perfumed, acidic, or exfoliating products on the treated area, as they may cause irritation; Petition 870250017410, dated 05 / 03 / 2025, page 29 / 41 20 / 26 • If any adverse reaction is noticed, such as an allergic reaction, treatment should be discontinued and the patient should contact the specialist to seek alternative dermocosmetics associated with photosensitizers to continue treatment; • In the tests we conducted, there was concern about the color of the final compounds, which can be bluish (when used with methylene blue or toluidine blue), or greenish when using malachite green. It was observed that, after application, within 3 minutes there is no longer a risk of these colors staining clothing. The applications last on average much longer than that, so this concern should not exist; • The skin may be stained with a bluish or greenish discoloration after the session, but this discoloration comes off easily with soap and water. However, we do not recommend washing the area after the session, and this discoloration disappears on its own within 12 hours after the initial use; • In the case of compounds with color, it can be recommended that the user only use our dermocosmetic during phototherapy sessions, and a neutral, colorless moisturizer at home.

[043] During treatment with photodynamic therapy in conjunction with dermocosmetics and photosensitizers for pain (of muscular or inflammatory origin) or muscle injury, it is essential to follow some guidelines to ensure effective recovery and avoid complications. In case of pain or muscle injury, patients should avoid: • Avoid applying perfumed, acidic, or exfoliating products to the treated area, as they may cause irritation. Petition 870250017410, dated 05 / 03 / 2025, pages 30 / 41 21 / 26 • Prolonged sun exposure in the treated area without adequate protection. • Intense physical activities that may cause excessive sweating in the first 24 hours after the session. • Vigorous massages or application of excessive heat to the affected area.

[044] For muscle injuries, it is important to avoid activities that may aggravate the condition. This includes: • Exercises with heavy loads, even if minimal. • Avoid intense aerobic activities, such as running and cycling, in the first few days after the injury. • Anaerobic exercises that require vigorous muscle contractions. • Isometric or isokinetic exercises without professional guidance. • Any activity that causes pain or discomfort in the affected limb.

[045] In the first few days after the injury, the focus should be on resting the affected limb to allow for initial healing. During this period, only photonic therapy should be performed along with the indicated dermocosmetic to accelerate recovery.

[046] Continue photodynamic therapy in conjunction with dermocosmetics. After the initial rest phase, gentle, non-impact movements can be introduced. The dermocosmetic should be used even on days without photodynamic therapy. Examples include: • Gentle stretches to prevent muscle stiffness. • Passive movement of the affected limb without resistance. Petition 870250017410, dated 05 / 03 / 2025, pages 31 / 41 22 / 26 • Light walks, gradually increasing the duration as tolerated.

[047] Continue photodynamic therapy in conjunction with dermocosmetics. When authorized by the specialist, low-intensity exercises can be started to strengthen the muscle without overloading it. The dermocosmetic should be used even on days without photodynamic therapy. Examples include: • Resistance exercises using low-resistance elastic bands. • Bodyweight exercises, such as standing up from a chair or slowly going up and down stairs. • Low-impact activities, such as swimming or water aerobics.

[048] Continue photodynamic therapy in conjunction with dermocosmetics. As recovery progresses, the intensity of the exercises should be gradually increased, always under the guidance of the specialist. The dermocosmetic should be used even on days without photodynamic therapy. Examples include: • Adding light weights to resistance exercises. • Moderate aerobic exercise, such as cycling on flat terrain. • Functional exercises, such as squats and controlled leg raises.

[049] In the final phase, the goal is to safely return to full training. Continue using photodynamic therapy in conjunction with dermocosmetics as needed (if clinical symptoms improve, you can stop) and follow the specialist's instructions. Examples include: Petition 870250017410, dated 05 / 03 / 2025, pages 32 / 41 23 / 26 • Resistance exercises with moderate to heavy weights, according to tolerance. • Intense aerobic activities, such as running and cycling on varied terrain. • Specific exercises for the patient's sport or routine activity.

[050] One of the objectives of the invention proposed here is to provide a PDT-type therapeutic treatment that uses a combination of a photosensitizing agent and a light source to activate the therapeutic effect of a dermocosmetic product. This combination will allow for greater bioavailability and deeper absorption of the other components of the pharmaceutical formulation in creams, ointments, or gels. The choice of the appropriate light beam is fundamental for the compound to be activated and exert its therapeutic action.

[051] Among the advantages of the solution proposed here, we can highlight the following: It allows greater penetration of the product into the deeper layers of the skin, increasing its effectiveness and reducing the risks of irritation or allergy on the surface; It stimulates cell regeneration and the formation of collagen and elastin, improving the firmness, elasticity and appearance of the skin, in addition to promoting wound healing and recovery from muscle or joint injuries; It has a selective action, acting only on altered or damaged cells, preserving healthy cells and surrounding tissue. This minimizes side effects and complications of the therapy; and it has an antibacterial, antifungal and anti-inflammatory effect, preventing or treating infections, inflammations or allergic processes that may compromise the health and beauty of the skin or the recovery from injuries. Petition 870250017410, dated 05 / 03 / 2025, pages 33 / 41 24 / 26

[052] Therefore, in order to allow for a better elucidation of the object in question, its description will be carried out with reference to the following figures and tables, where: Figure 1 illustrates a sensitivity graph for different wavelengths; Figure 2 illustrates a table relating photosensitizing agents and the respective wavelength ranges recommended for their activation.

[053] In accordance with what is illustrated in the figures mentioned above, the “compound and photodynamic therapy employing SPECIFIC CHEMICAL COMPOUNDS FOR DERMOCOSMETICS”, object of this Patent of Invention, consists of a compound for therapeutic treatment of the PDT type that uses the combination of a photosensitizing agent and a light source to activate the therapeutic effect of a dermocosmetic product.

[054] Propolis was used in concentrations up to 2%, together with urea, panthenol, azelaic acid, aloe vera, and tocopherol, used with a green light beam in the range of 515nm to far-infrared; methylene blue was used in concentrations up to 2%, together with other drugs such as hyaluronic acid, salicylic acid, arnica montana, centella asiatica, clindamycin, coenzyme Q10, glycerin, ibuprofen, niacinamide (vitamin B3), nimesulide, jojoba oil, peptides, and benzoyl peroxide, methyl salicylate, used with a red light beam in the range of 600nm to far-infrared; malachite green was used in concentrations between 1% and 2%, together with aloe vera and ceramide, used with a blue light beam in the range of Petition 870250017410, dated 05 / 03 / 2025, pages 34 / 41 25 / 26 490nm to far-infrared; Hematoporphyrin along with Vitamin K, with a red light beam, in the range of 600nm to far-infrared; and Toluidine Blue, in concentrations up to 1%, along with Niacinamide (Vitamin B3), Lactobionic Acid, Rosehip Oil, Lactic Acid and Caffeine, used with a red light beam in the range of 612nm to far-infrared, considering that all lower ranges also have an action in the photodynamic therapy of dermocosmetics with this component.

[055] When testing chemical portions and variances using spectrophotometry, the behavior is modified by the wavelength employed. The photonic reaction triggered in the process will activate the dermocosmetics used. Malachite green, toluidine blue, methylene blue, propolis, and hematoporphyrin are the so-called photonic hosts, a photosensitizing agent that creates a medium for light to reach and activate the treatment substances, which is the main characterizing element of the invention proposed here. The treatment substance penetrates the epidermis poorly and its penetrability is slow due to aspects of chemical passage. When the macromolecule is altered by the use of a photonic host, the substance accelerates its passage through the larger epidermis, with more free valence, increasing its penetration.

[056] Here we therefore highlight the importance of modifying the behavior of chemical substances through combined use with lights of specific wavelengths, mentioning the activation of substances by means of light and the influence of different factors in this process, such as the addition of another substance to the. Petition 870250017410, dated 05 / 03 / 2025, pages 35 / 41 26 / 26 formulation, the photosensitizing agent. The patent application proposed here involves the use of a specific substance, along with its own photosensitizing agent, within specific light ranges.

[057] The therapy in question can be used in the areas of dermocosmetics, sports injuries, cell regeneration, wound healing or mesotherapy, bringing some advantages over existing products that do not use light beams.

[058] This descriptive report dealt with a new “PHOTODYNAMIC COMPOUND AND THERAPY USING SPECIFIC CHEMICAL COMPOUNDS FOR DERMOCOSMETICS”, presenting, as can be seen from the description and figures presented, some advantages and differentiated applications in relation to existing conventional methods.

[059] Having, therefore, described and illustrated the best embodiment currently contemplated for the realization of the present Invention Patent, numerous modifications and variations in its embodiment may be readily introduced by technicians and specialists in the field. However, it is understood that the present invention is not limited to the practical aspects illustrated and described in this report, encompassing within its scope all obvious variations and modifications not described, such as, for example, the application methods employed.

[060] Because of the advantages it offers and also because it is novel, has industrial application and is inventive, it fulfills all the requirements and meets the necessary conditions to obtain an Invention Patent. Petition 870250017410, dated 05 / 03 / 2025, pp. 36 / 41

Claims

1 / 2 CLAIMS 1.- “PHOTODYNAMIC COMPOUND AND THERAPY EMPLOYING specific chemical compounds for dermocosmetics”, characterized by including propolis, methylene blue and toluidine blue as photosensitizers in dermocosmetic and cosmetic formulations (ointments, creams, lotions and oils) so that, under the incidence of the correct light beams (generated by LEDs or Laser), they potentiate the action of other dermocosmetic or cosmetic compounds applied to the epidermis of patients undergoing treatment.

2. "PHOTODYNAMIC COMPOUND AND THERAPY EMPLOYING specific chemical compounds for dermocosmetics", according to Claim 1, characterized by employing the following photosensitizing agents: Propolis, in concentrations up to 2%, together with urea, panthenol, azelaic acid, aloe vera and tocopherol, used together with a green light beam, in the range of 515nm to far infrared; Methylene blue, in concentrations up to 2%, along with other drugs such as hyaluronic acid, salicylic acid, arnica montana, centella asiatica, clindamycin, coenzyme Q10, glycerin, ibuprofen, niacinamide (vitamin B3), nimesulide, jojoba oil, peptides and benzoyl peroxide, methyl salicylate, used with a red light beam, in the range of 600nm to far infrared; Malachite green, in concentrations between 1% and 2%, along with aloe vera and ceramide, used with a blue light beam in the range of 490nm to far infrared;Hematoporphyrin together with Vitamin K, using a red light beam in the range of 600nm to far-infrared; and Toluidine Blue, in concentrations up to 1%, together with Niacinamide (Vitamin B3), Lactobionic Acid, Rosehip Oil, Lactic Acid and Caffeine, used with a red light beam in the range of 612nm to far-infrared. Petition 870250017410, dated 05 / 03 / 2025, p. 38 / 41;

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