Inoculant composition, coated plant seed, kit, and, method
Patent Information
- Application Number
- BR112019024749
- Authority / Receiving Office
- BR · BR
- Patent Type
- Patents
- Current Assignee / Owner
- Publication Date
- 2026-09-15
AI Technical Summary
Existing inoculant compositions for agriculturally beneficial microorganisms face challenges in maintaining the stability and survival of microbial cells and spores, which affects their effectiveness in agricultural applications.
Incorporation of paraffin oils and/or waxes into inoculant compositions to stabilize microbial cells/spores, along with dispersants and other carrier components, to enhance stability and survival.
The use of paraffin oils and waxes significantly improves the stability and survival of microbial cells/spores, leading to enhanced plant growth and yield by ensuring the viability of microorganisms under various environmental conditions.
Abstract
Description
Federative Republic of Brazil Ministry of Economy National Institute of Industrial Property (21) BR 112019024749-1 A2 ||||||||||||||| *BR112O19O24749A2 (22) Deposit Date: 05 / 24 / 2018 (43) National Publication Date: 06 / 09 / 2020 (54) Title: USE OF ONE OR MORE PARAFFIN OILS AND / OR WAXES, LIQUID INOCULANT COMPOSITION, COATED PLANT SEED, KIT, AND, METHOD (51) Int. CL: C12R 1 / 645; A01N 02 / 25; A01N 63 / 00; C12N 1 / 04; C12N 1 / 14; 🇧🇷 (30) Unionist Priority: 5 / 26 / 2017 US 62 / 511434. (71) Depositor(s): NOVOZYMES BIOAG A / S. (72) Inventor(s): DAN CLARY; BEN DOUGHAN. (86) PCT Order: PCT US2018034381 of 05 / 24 / 2018 (87) PCT Publication: WO 2018 / 218016 of 11 / 29 / 2018 (85) Date of the National Phase: 11 / 25 / 2019 (57) Summary: The present disclosure provides inoculant compositions and methods for improving the survival and / or stability of microbial cells and / or spores in an inoculant composition. In some embodiments, inoculant compositions of the present disclosure comprise microbial cells and / or spores in a carrier comprising paraffin oils and / or waxes. USE OF ONE OR MORE PARAFFIN OILS AND / OR WAXES, LIQUID INOCULANT COMPOSITION, COATED PLANT SEED, KIT, AND, METHOD NAMES OF THE PARTIES FOR A JOINT RESEARCH AGREEMENT
[001] The concepts of the invention described in this document were developed as part of a joint research agreement between Monsanto Company and Novozymes BioAg A / S. The activities that generated the claimed invention were carried out within the scope of the joint research agreement, where said agreement was in effect on or before the date on which the claimed invention was produced. CROSS REFERENCE TO RELATED ORDER
[002] This application claims the benefit of the patent application provisional n 2 U.S. 62 / 511,434, filed on May 26, 2017, whose disclosure is incorporated into this document by way of reference in its entirety. FIELD OF THE INVENTION
[003] The present disclosure relates to compositions and methods for to improve the stability and survival of microbial cells and / or spores in inoculant compositions. BACKGROUND
[004] The inoculant compositions comprising micro Agriculturally beneficial organisms are known in the art. See, for example, US patents. 5,484,464; 5,586,411; 5,695,541; 5,804,208; 5,916,029; 6,569,425; 6,808,917; 6,824,772; 7,429,477; 8,148,138; 8,278,247; 8,445,256; 8,883,679; 8,921,089; 8,999,698; 9,017,442; 9,101,088; 9,234,251; 9,340,464.
[005] Due to the fact that the effectiveness of such inoculant compositions usually depends on the ability of the microorganisms in them to survive and propagate the following application, much effort has been made to increase the stability of agriculturally beneficial microorganisms in inoculant compositions. See, for example, US patents. 8,011,132 and 9,090,884.
[006] However, there is still a need for compositions and methods improved to improve the stability and survival of microorganisms in inoculant compositions. SUMMARY OF THE CLAIMED INVENTION
[007] The present disclosure provides innovative and inventive uses for paraffin oils and waxes.
[008] A first aspect of the present disclosure is the use of one or more plus oils and / or paraffin waxes to stabilize microbial cells / spores.
[009] A second aspect of the present disclosure is a composition liquid inoculant comprising one or more oils and / or paraffin waxes. In some embodiments, the inoculant composition comprises one or more dispersants.
[0010] A third aspect of the present disclosure is a plant seed coated with an inoculant comprising microbial cells / spores and one or more oils and / or paraffin waxes.
[0011] A fourth aspect of the present disclosure is a kit comprising coated plant seed housed in a container.
[0012] A fifth aspect of the present disclosure is a method for applying a liquid inoculant composition comprising microbial cells / spores and one or more oils and / or paraffin waxes to a plant propagation material (e.g., seed) and / or or a plant growing from said plant propagation material.
[0013] A sixth aspect of the present disclosure is a method for applying a liquid inoculant composition comprising microbial cells / spores and one or more oils and / or paraffin waxes to a medium of plant growth (eg soil). BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 is a graph showing the seed survivability of P. bilaiae spores at 30 °C and 65% relative humidity in SUN AG® 7N supplemented with ATLOX™ 4912 (white diamonds, trend line dotted), SUN AG® 7N supplemented with ATLOX™ 4912 and tung oil (white triangles, solid black trend line), SUN AG® 7N supplemented with SUNWAX™ DP 116 (gray circles, solid gray trend line), SUN AG ® 7N supplemented with SUNWAX™ DP 116 and tung oil (grey squares, dotted gray trend line), SUN AG® 7N supplemented with SUNWAX™ DP 116 and SiO2 (grey triangles, dashed gray trend line) or SUN AG® 7N supplemented with SUNWAX™ DP 116, SiO2 and tung oil (closed squares, dashed black trend line), compared to a commercially available inoculant comprising spores of P. bilaiae (open circles, dashed black trend line).
[0015] Figure 2 is a graph showing the seed survivability of spores of P. bilaiae at 10 °C and 50% relative humidity in SUN AG® 7N (black squares, dashed black trend line) or SUN AG® 7N supplemented with tung oil (grey triangles, solid black trend line), compared to a commercially available inoculant comprising spores of P. bilaiae (white diamonds, dotted black trend line). DETAILED DESCRIPTION
[0016] This description is not intended to be a detailed catalog of all the different ways in which the invention can be implemented or of all the features that can be added to the present invention. For example, features illustrated in relation to one embodiment may be incorporated into other embodiments, and features illustrated in relation to one specific modality can be deleted from that modality. Furthermore, various variations and additions to the various embodiments suggested herein, which do not depart from the present invention, will be apparent to those skilled in the art in light of the present disclosure. Therefore, the following description is intended to illustrate some specific embodiments of the invention and not exhaustively specify all permutations, combinations and variations thereof.
[0017] The terminology used herein is for the purpose of describing specific embodiments only and is not intended to limit the invention.
[0018] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by an individual of ordinary skill in the art to which this invention belongs. It will furthermore be understood that terms, as defined in commonly used dictionaries, are to be interpreted as having a meaning that is consistent with their meaning in the context of the descriptive report and relevant technique and are not to be interpreted in an idealized or excessively formal unless expressly defined in this document. For the sake of brevity and / or clarity, well-known functions or constructs may not be described in detail.
[0019] As used in this document, the singular forms “an”, “an”, “the” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise.
[0020] As used herein, the terms "acaricide" and "anti-mite" refer to an agent or combination of agents the application of which is toxic to a mite (i.e., kills a mite, inhibits the growth of a mite, and / or inhibits the reproduction of an acarid).
[0021] As used in this document, the term “agent agriculturally beneficial” refers to any agent (e.g., chemical or biological agent) or combination of agents whose application causes or provides a beneficial and / or useful effect in agriculture including, without limitation, agriculturally beneficial microorganisms, biostimulants, nutrients, pesticides (e.g., acaricides, fungicides, herbicides, insecticides, and nematicides) and plant signal molecules.
[0022] As used herein, the term “agriculturally beneficial microorganism” refers to a microorganism that has at least one agriculturally beneficial property (for example, the ability to fix nitrogen, the ability to solubilize phosphate and / or the ability to ability to produce an agriculturally beneficial agent, such as a plant signal molecule).
[0023] As used herein, the term "agriculturally acceptable carrier" refers to a substance or composition that can be used to deliver an agriculturally beneficial agent to a plant, plant part, or plant growth medium (e.g., soil) without causing / having an unduly adverse effect on plant growth and / or yield. As used herein, the term "leaf compatible carrier" refers to a material that can be foliarly applied to a plant or plant part without causing / having an excessively adverse effect on the plant, plant part, plant growth , plant health or similar. As used herein, the term "seed compatible carrier" refers to a material that can be applied to a seed without causing / having an unreasonably adverse effect on the seed, the plant growing from the seed, seed germination or similar. As used herein, the term "soil compatible carrier" refers to a material that can be added to a soil without causing / having an excessively adverse effect on plant growth, soil structure, soil drainage or the like.
[0024] As used in this document, the term “and / or” is intended to include any and all combinations of one or more of the associated listed items, as well as the absence of combinations when interpreted in the alternative (“or” ).
[0025] As used herein, the terms "associated with", "in association with" and "associated therewith", when used in reference to a relationship between a microbial strain or inoculant composition of the present disclosure and a plant or part of plant, refer to at least a juxtaposition or extreme proximity of the microbial strain or inoculant composition and the plant or plant part. Such juxtaposition or extreme proximity may be achieved by contacting or applying the microbial strain or inoculant composition directly to the plant or plant part and / or applying the microbial strain or inoculant composition to the plant growth medium (e.g. example, soil) in which the plant or plant part will grow (or is currently growing). According to some embodiments, the microbial strain or inoculant composition is applied as a coating to the outer surface of the plant or plant part. According to some embodiments, the microbial strain or inoculant composition is applied to the soil at, near or surrounding the site where the plant or plant part will grow (or is currently growing).
[0026] As used herein, the term "aqueous" refers to a composition that contains more than a trace amount of water (i.e., more than 0.5% water by weight, based on the total weight of the composition).
[0027] As used herein, the term “biostimulant” refers to an agent or combination of agents whose application enhances one or more metabolic and / or physiological processes of a plant or plant part (e.g., carbohydrate biosynthesis, ion uptake, nucleic acid uptake, nutrient delivery, photosynthesis and / or respiration).
[0028] As used in this document, the term “BRADY” shall be interpreted as an abbreviated substitute for the phrase “Bradyrhizobium elkanii SEMIA 501, Bradyrhizobium elkanii SEMIA 587, Bradyrhizobium elkanii SEMIA 5019, Bradyrhizobium japonicum NRRL B-50586 (also deposited as NRRL B-59565), Bradyrhizobium japonicum NRRL B-50587 (also deposited as NRRL B-59566), Bradyrhizobium japonicum NRRL B-50588 (also deposited as NRRL B-59567), Bradyrhizobium japonicum NRRL B-50589 (also deposited as NRRL B -59568), Bradyrhizobium japonicum NRRL B-50590 (also deposited as NRRL B-59569), Bradyrhizobium japonicum NRRL B-50591 (also deposited as NRRL B-59570), Bradyrhizobium japonicum NRRL B-50592 (also deposited as NRRL B-59571 ), Bradyrhizobium japonicum NRRL B-50593 (also deposited as NRRL B-59572), Bradyrhizobium japonicum NRRL B-50594 (also deposited as NRRL B-50493), Bradyrhizobium japonicum NRRL B-50608, Bradyrhizobium japonicum NRRL B-50609, Bradyrhizobium japonicum NRRL B-50610, Bradyrhizobium japonicum NRRL B-50611, Bradyrhizobium japonicum NRRL B-50612, Bradyrhizobium japonicum NRRL B-50726, Bradyrhizobium japonicum NRRL B-50727, Bradyrhizobium japonicum NRRL B-50728, Bradyrhizobium japonicum NRRL B-50729, Bradyrhizobium japonicum NRRL B-50730, Bradyrhizobium japonicum SEMIA 566, Bradyrhizobium japonicum SEMIA 5079, Bradyrhizobium japonicum SEMIA 5080, Bradyrhizobium japonicum USDA 6, Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 122, Bradyrhizobium japonicum USDA 123, Bradyrhizobium japonicum USDA 127, Bradyrhizobium japonicum USDA 129 and / or Bradyrhizobium japonicum USDA 532C”.
[0029] As used herein, the terms “colony forming unit” and “cfu” refer to a microbial cell / spore capable of propagating in or within a medium or substrate of suitable growth (eg, a soil) when conditions (eg, temperature, humidity, nutrient availability, pH, etc.) are favorable for germination and / or microbial growth.
[0030] As used herein, the term "consists essentially of", when used in reference to inoculant compositions and methods of the present disclosure, means that the compositions / methods may contain additional components / steps so long as the additional components / steps do not materially alter the composition / method. The term "materially alter" as applied to a composition / method of the present disclosure refers to an increase or decrease in the effectiveness of the composition / method of at least 20%. For example, a component added to an inoculant composition of the present disclosure can be considered to "materially alter" the composition if it increases the stability and / or survivability of the microbial cells / spores in the inoculant composition by at least 20% and / or or if it increases or decreases the ability of the composition to improve corn yield by at least 20%.
[0031] As used herein, the term “diazotroph” refers to an organism with the ability to convert atmospheric nitrogen (N2) into a form that can be utilized by a plant or plant part (e.g., ammonia (NH3) , ammonium (NH4+), etc.).
[0032] As used herein, the term “dispersant” refers to an agent or combination of agents, the application of which reduces the cohesion of similar particles, the surface tension of a liquid, the interfacial tension between two liquids and / or the interfacial tension between a liquid and a solid.
[0033] As used herein, the terms “effective amount”, “effective concentration” and “effective amount / concentration” refer to an amount or concentration that is sufficient to cause a desired effect (e.g., improved corn yield ). The absolute value of amount / concentration that is sufficient to cause the desired effect can be affected by factors such as the type and magnitude of effect desired, the type, size and volume of material to which the inoculating composition will be applied, the type (or types) of micro- organisms in the composition, the number of microorganisms in the composition, the stability of the microorganism (or microorganisms) in the inoculant composition, and the storage conditions (eg, temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount / concentration using routine dose response experiments after studying the present disclosure.
[0034] As used herein, the term "enhanced dispersal" refers to an improvement in one or more microbial dispersal characteristics compared to one or more controls (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it is devoid of one or more of the components found in the inoculant composition of the present disclosure). Exemplary microbial dispersion characteristics include, but are not limited to, the percentage of microbes that exist as single cells / spores when the inoculant composition is diluted with water. An inoculant composition that improves one or more microbial dispersal characteristics of the microorganism (or microorganisms) contained therein compared to a control composition (e.g., a control composition that is identical to the inoculant composition except that that it is devoid of one or more of the components found in the inoculant composition) provides improved dispersion and may be termed a "readily dispersible inoculant composition".
[0035] As used herein, the terms "enhanced growth" and "enhanced plant growth" refer to an improvement in one or more characteristics of plant growth and / or development in comparison with one or more control plants (e.g. a plant germinated from an untreated seed or an untreated plant). Exemplary plant growth / developmental characteristics include, but are not limited to, biomass, carbohydrate biosynthesis, chlorophyll content, cold tolerance, drought tolerance, height, leaf length, leaf mass, number of leaves, surface area leaf volume, leaf volume, nutrient uptake (e.g., calcium, magnesium, nitrogen, phosphorus, and / or potassium uptake), rate(s) of photosynthesis, root area, root diameter, root length, root mass roots, root nodulation (eg nodule mass, nodule number, nodule volume), root number, root surface area, root volume, salt tolerance, seed germination, seedling emergence, shoot diameter , shoot length, shoot mass, number of shoots, shoot surface area, shoot volume, dissemination, stomatal conductance and survival rate. Unless otherwise indicated, references to enhanced plant growth should be interpreted to mean that the microbial strains, inoculant compositions and methods of the present disclosure enhance corn plant growth by enhancing nutrient availability, improving soil characteristics, etc. and should not be interpreted as suggesting that the microbial strains, inoculant compositions and methods of the present disclosure act as plant growth regulators.
[0036] As used herein, the terms "enhanced stability" refer to an improvement in one or more microbial stability characteristics compared to one or more controls (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure). Exemplary microbial stability characteristics include, but are not limited to, the ability to germinate and / or propagate after being coated onto a seed and / or stored for a defined period of time, and the ability to cause a desired effect (e.g., improved plant yield and / or increased pesticidal activity) after being coated onto a seed and / or stored for a defined period of time. A microorganism that exhibits improvement in one or more microbial stability characteristics compared to a control microorganism when each is subjected to the same conditions (e.g. seed coating and storage conditions) exhibits improved stability and may be termed “stable microorganism”. An inoculant composition that improves one or more microbial stability characteristics of the microorganism (or microorganisms) contained therein compared to a control composition (e.g., a control composition that is identical to the inoculant composition except that that it is devoid of one or more of the components found in the inoculant composition) provides improved stability and may be referred to as a "stable inoculant composition".
[0037] As used herein, the terms "enhanced survival" and "improved microbial survival" refer to an improvement in the survival rate of one or more microorganisms in an inoculant composition compared to one or more microorganisms in a control composition (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure). An inoculant composition that improves the survival rate of one or more of the microorganisms contained herein with a control composition (e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition) provides improved survival and can be termed a stable inoculant composition.
[0038] As used herein, the terms "enhanced yield" and "enhanced plant yield" refer to an improvement in one or more plant yield characteristics compared to one or more control plants (e.g., a control plant germinated from an untreated seed). Exemplary plant yield characteristics include, but are not limited to, biomass; bushels per acre; grain weight per plot (GWTPP); nutritional content; percentage of plants in a given area (eg terrain) that fail to produce grain; yield in percent moisture standard (YSMP), such as grain yield in percent moisture standard (GYSMP); yield per field (YPP), such as grain weight per field (GWTPP); and yield reduction (YRED). Unless otherwise indicated, references to improved plant yield should be interpreted to mean that the microbial strains, inoculant compositions and methods of the present disclosure enhance plant yield by enhancing nutrient availability, improving plant characteristics, soil, etc. and should not be interpreted as suggesting that the microbial strains, inoculant compositions and methods of the present disclosure act as plant growth regulators.
[0039] As used herein, the term "foliage" refers to those portions of a plant that normally grow above the ground, including, but not limited to, leaves, stems, stems, flowers, fruiting bodies, and fruits.
[0040] As used herein, the terms “foliar application” and “foliar application” refer to the application of one or more active ingredients to the foliage of a plant (eg, to the leaves of the plant). Application may be effected by any suitable means, including, but not limited to, spraying the plant with a composition comprising the ingredient active ingredient (or active ingredients). In some embodiments, the active ingredient (or active ingredients) is applied to the leaves, stems and / or stem of the plant and not to the flowers, fruiting bodies or fruits of the plant.
[0041] As used herein, the terms “fungicide” and “antifungal” refer to an agent or combination of agents whose application is toxic to a fungus (i.e., kills a fungus, inhibits the growth of a fungus, and / or inhibits the reproduction of a fungus).
[0042] As used herein, the term “fulvic acid” encompasses both pure fulvic acids and fulvic acid salts (fulvates). Non-limiting examples of fulvic acids include ammonium fulvate, boron fulvate, potassium fulvate, sodium fulvate and the like. In some embodiments, the fulvic acid comprises, consists essentially of or consists of MDL Number MFCD09838488 (CAS Number 479-66-3).
[0043] As used herein, the terms "herbicide" and "herbicide" refer to an agent or combination of agents the application of which is toxic to a weed (i.e., kills a weed, inhibits the growth of a weed, and / or inhibits reproduction of a weed).
[0044] As used herein, the term "humic acid" encompasses pure humic acids and humic acid salts (humates). Non-limiting examples of humic acids include ammonium humate, boron humate, potassium humate, sodium humate and the like. In some embodiments, the humic acid comprises, consists essentially of, or consists of one or more of MDL number MFCD00147177 (CAS number 1415-93-6), MDL number MFCD00135560 (CAS number 68131-04-4), MDL number MFCS22495372 (CAS number 68514-28-3), CAS number 93924-35-7, and CAS number 308067-45-0.
[0045] As used herein, the terms "inoculating composition" and "inoculum" refer to a composition comprising microbial cells and / or spores, said cells / spores having the ability to to propagate / germinate in or on a suitable growth medium or substrate (e.g. a soil) when conditions (e.g. temperature, humidity, nutrient availability, pH, etc.) are favorable for germination and / or microbial growth.
[0046] As used herein, the terms "insecticide" and "anti-insect" refer to an agent or combination of agents whose application is toxic to an insect (i.e., kills an insect, inhibits the growth of an insect, and / or inhibits reproduction of an insect).
[0047] As used herein, the term "isomer" includes all stereoisomers of the compounds and / or molecules to which it refers, including enantiomers and diastereomers, as well as all conformers, rotamers and tautomers, unless otherwise indicated in contrary. The compounds and / or molecules disclosed herein include all enantiomers in substantially pure levorotatory or dextrorotatory form, or in a racemic mixture, or in any ratio of enantiomers. Where embodiments disclose a (D)-enantiomer, this embodiment also includes the (L)-enantiomer; where embodiments disclose an (L)-enantiomer, that embodiment also includes the (D)-enantiomer. Where embodiments disclose a (+)-enantiomer, this embodiment also includes the (-)-enantiomer; where embodiments disclose a (-)-enantiomer, that embodiment also includes the (+)-enantiomer. Where embodiments disclose an (S)-enantiomer, this embodiment also includes the (R)-enantiomer; where embodiments disclose an (R)-enantiomer, that embodiment also includes the (S)-enantiomer. Embodiments are intended to include any diastereomers of the compounds and / or molecules referred to herein in diastereomerically pure form and in admixture form in all ratios. Unless stereochemistry is explicitly indicated in a chemical structure or chemical name, the chemical structure or chemical name is intended to encompass all possible stereoisomers, conformers, rotamers, and tautomers of compounds and / or molecules illustrated.
[0048] As used herein, the term "modified microbial strain" refers to a microbial strain that is modified from a strain isolated from nature. The modified microbial strains can be produced by any method (or methods) including, but not limited to, chemical mutation or other form of induced mutation of a polynucleotide within any genome within the strain; the insertion or deletion of one or more nucleotides within any genome within the strain or combinations thereof; an inversion of at least one DNA segment within any genome within the strain; a rearrangement of any genome within the strain; generalized or specific transduction of homozygous or heterozygous polynucleotide segments into any genome within the strain; introducing one or more phages into any genome of the strain; transformation of any strain that results in the introduction of stable replicating autonomous extrachromosomal DNA into the strain; any change in any genome or total DNA composition within the strain isolated from nature as a result of conjugation with any different microbial strain; and any combination of the foregoing. The term modified microbial strain includes a strain with (a) one or more heterologous nucleotide sequences, (b) one or more non-naturally occurring copies of a nucleotide sequence isolated from nature (i.e., additional copies of a naturally occurring gene in the microbial strain from which the modified microbial strain was derived), (c) an absence of one or more nucleotide sequences that would otherwise be present in the natural reference strain, for example, by nucleotide sequence deletion, and ( d) Added extrachromosomal DNA. In some embodiments, the modified microbial strains comprise a combination of two or more nucleotide sequences (e.g., two or more naturally occurring genes that do not naturally occur in the same microbial strain), or comprise a nucleotide sequence isolated from nature at a locus that is different from the natural locus.
[0049] As used herein, the terms “nematicide” and “vermicide” refer to an agent or combination of agents the application of which is toxic to a nematode (i.e., kills a nematode, inhibits the growth of a nematode and / or inhibits reproduction of a nematode).
[0050] As used herein, the term “nitrogen-fixing organism” refers to an organism with the ability to convert atmospheric nitrogen (N2) into a form that can be utilized by a plant or plant part (e.g., ammonia (NH3), ammonium (NH / ), etc.).
[0051] As used herein, the term "non-aqueous" refers to a composition that contains at most a trace amount of water (i.e., at most 0.5% water by weight, based on the total weight of composition).
[0052] As used in this document, the term “nutrient” refers to a compound or element useful for plant nutrition (for example, vitamins, macrominerals, micronutrients, trace minerals, organic acids, etc. that are necessary for the plant growth and / or development).
[0053] As used in this document, the term “PENI” should be interpreted as an abbreviated substitute for the phrase "P. bilaiae ATCC 18309, P. bilaiae ATCC 50162, P. bilaiae NRRL 50777, P. bilaiae NRRL 50780, P. bilaiae NRRL 50783, P. bilaiae NRRL 50786, P. bilaiae NRRL 67154, P. bilaiae NRRL 20851, P. bilaiae ATCC 50169, P. bilaiae NRRL 50778, P. bilaiae NRRL 50781, P. bilaiae NRRL 50784, P. bilaiae NRRL 50787, P. bilaiae NRRL 67155, P. bilaiae NRRL 22348, P. bilaiae NRRL 50776, P. bilaiae NRRL 50779, P. bilaiae NRRL 50782, P. bilaiae NRRL 50785, P. bilaiae NRRL 50788, P. bilaiae NRRL 67156, P. bilaiae NRRL 67157, P. bilaiae NRRL 67158, P. bilaiae NRRL 67159, P. bilaiae RS7B-SD1, Penicillium brevicompactum AgRF18, Penicillium canescens ATCC 10419, Penicillium expansum ATCC 24692, Penicillium expansum YT02, Penicillium fellatanum ATCC 48694, Penicillium gaestrivorus NRRL 50170 , Penicillium glabrum DAOM 239074, Penicillium glabrum CBS 229,28, Penicillium janthinellum ATCC 10455, Penicillium lanosocoeruleum ATCC 48919, Penicillium radicum ATCC 201836, Penicillium radicum FRR 4717, Penicillium radicum FRR 4719, Penicillium radicum N93 / 47267, and / or Penicillium raistrickii ATCC 10490."
[0054] As used herein, the terms “Penicillium bilaiae” and “P. bilaiae” are intended to include all iterations of the species name, such as “Penicillium bilajf and “Penicillium bilaii”.
[0055] As used herein, the terms "percent identity", "% identity" and "percent identical" refer to the relatedness of two or more nucleotide or amino acid sequences, which can be calculated by (i) comparing two sequences optionally aligned in a comparison window, (ii) determining the number of positions at which the identical nucleic acid base (for nucleotide sequences) or amino acid residue (for proteins) occurs in both sequences to generate the number of compatible positions by (iii) dividing the number of compatible positions by the total number of positions in the comparison window and then (iv) multiplying this quotient by 100% to generate the percent identity. If "percent identity" is being calculated against a reference sequence without a specific comparison window being specified, then percent identity is determined by dividing the number of matched positions in the alignment region by the total length of the reference sequence. Consequently, for the purposes of the present invention, when two strings (query and subject) are ideally aligned (allowing for gaps in their alignment), the "percent identity" for the query string is equal to the number of identical positions between the two sequences divided by the total number of positions in the query sequence over its length (or a comparison window), which is then multiplied by 100%.
[0056] As used herein, the term "pest" includes any organism or virus that adversely affects a plant, including, but not limited to, organisms and viruses that spread disease, damage host plants, and / or compete for soil nutrients . The term "pest" encompasses organisms and viruses that are known to associate with plants and to have a detrimental effect on plant health and / or vigor. Plant pests include, but are not limited to, arachnids (e.g., mites, ticks, spiders, etc.), bacteria, fungi, gastropods (e.g., slugs, snails, etc.), invasive plants (e.g., weeds), insects (e.g. whiteflies, thrips, weevils, etc.), nematodes (e.g. root knot nematode, soybean cyst nematode, etc.), rodents and viruses (e.g. mosaic virus (TMV), tomato spotted wilt virus (TSWV), cauliflower mosaic virus (CaMV), etc.).
[0057] As used herein, the terms "pesticide" and "antipest" refer to agents or combination of agents whose application is toxic to a pest (i.e., kills a pest, inhibits the growth of a pest, and / or inhibits the reproduction of a pest). Non-limiting examples of pesticides include acaricides, fungicides, herbicides, insecticides and nematicides, etc.
[0058] As used herein, the term "phosphate solubilizing microorganism" refers to a microorganism with the ability to convert insoluble phosphate into a soluble form of phosphate.
[0059] As used herein, the term "plant" includes all plant populations, including, but not limited to, agricultural, horticultural, and forestry plants. The term “plant” includes plants obtained by conventional breeding and optimization methods (e.g. selection aided by markers) and plants obtained by genetic manipulation, including cultivars protected and not protected by plant breeders' rights.
[0060] As used herein, the term "plant cell" refers to a cell from an intact plant, a cell taken from a plant, or a cell derived from a cell taken from a plant. Thus, the term "plant cell" includes cells within seeds, suspension cultures, embryos, meristematic regions, callous tissue, leaves, shoots, gametophytes, sporophytes, pollen, and microspores.
[0061] As used herein, the term "plant growth regulator" refers to an agent or combination of agents whose application accelerates or retards the growth / maturation rate of a plant through direct physiological action on the plant or which otherwise alters the behavior of a plant through direct physiological action on the plant. “Plant growth regulator” shall not be construed to include any agent or combination of agents excluded from the definition of “plant regulator” that is set forth in section 2(v) of the Federal Insecticide, Fungicide, and Rodenticide Act (Title 7 of the U.S.C. § 136(v)). Thus, "plant growth regulator" does not encompass microorganisms applied to a plant, plant part or plant growth medium for the purpose of enhancing the availability and / or uptake of nutrients, nutrients necessary for the normal growth of the plant. plant, chemical soil conditioners applied for the purpose of improving soil characteristics favorable for plant growth, or vitamin hormone products as defined by Title 40 of the C.F.R. § 152.6(f).
[0062] As used herein, the term "plant part" refers to any part of a plant, including cells and tissues derived from plants. Thus, the term “plant part” can refer to any of the components or organs of plants (eg leaves, stems, roots, etc.), plant tissues, plant cells and seeds. Examples of plant parts include, but are not are limited to anthers, embryos, flowers, fruits, fruiting bodies, leaves, ovules, pollen, rhizomes, roots, seeds, shoots, stems and tubers, as well as trunks, rootstocks, protoplasts, calluses and the like.
[0063] As used herein, the term "plant propagation material" refers to a plant part from which an entire plant can be generated. Examples of plant propagation materials include, but are not limited to, cuttings (eg, leaves, stems), rhizomes, seeds, tubers, and cells / tissues that can be grown into a whole plant.
[0064] As used herein, the terms "spore" and "microbial spore" refer to a microorganism in its dormant, protected state.
[0065] As used herein, the term "stabilizing compound" refers to an agent or combination of agents whose application improves the survival and / or stability of a microorganism in an inoculating composition.
[0066] As used herein in connection with inoculant compositions, the term "stable" refers to an inoculant composition in which microorganisms exhibit enhanced stability and / or enhanced survival. In general, an inoculant composition can be marked as "stable" if it improves the survival rate and / or at least one microbial stability characteristic of at least one microorganism contained therein.
[0067] As used herein in relation to microbial strains, the term “survival rate” refers to the percentage of microbial cells / spores that are viable (i.e. have the ability to propagate in or within a medium or substrate of adequate growth (eg, a soil) when conditions (eg, temperature, humidity, nutrient availability, pH, etc.) are favorable for germination and / or microbial growth) in a given period of time.
[0068] While certain aspects of the present disclosure are hereinafter described with reference to its embodiments, it will be understood by those of ordinary skill in the art that various changes in form and detail can be made without departing from the spirit and scope of the present invention as defined by the claims.
[0069] All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety, except to the extent that they contradict any statement expressly set forth herein.
[0070] The present disclosure provides inoculant compositions comprising, consisting essentially of or consisting of one or more microbial cells / spores and a carrier comprising, consisting essentially of or consisting of one or more oils and / or paraffin waxes.
[0071] The inoculant compositions of the present disclosure may comprise any suitable microorganism (or suitable microorganisms), including, but not limited to, agriculturally beneficial microorganisms, such as diazotrophs, phosphate solubilizing microorganisms, and biopesticides. The selection of suitable microorganisms will depend on the intended application (or intended applications). In some embodiments, the inoculant compositions of the present disclosure comprise one or more microorganisms selected from the genera and species listed in Appendix A.
[0072] In some embodiments, the inoculant compositions of the present disclosure comprise one or more gram-negative bacteria and / or gram-positive bacteria. Non-limiting examples of bacteria that may be included in the compositions of the present disclosure include Azospirillum brasilense INTA Az-39, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens NRRL B 50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens MBI600, Bacillus amyloliquefaciens BS27 (depositada como NRRL B-5015), Bacillus amyloliquefaciens BS2084 (depositada como NRRL B-50013), Bacillus amyloliquefaciens 15AP4 (deposited as ATCC PTA-6507), Bacillus amyloliquefaciens 3AP4 (deposited as ATCC PTA-6506), Bacillus amyloliquefaciens LSSA01 (deposited as NRRL B-50104), Bacillus amyloliquefaciens ABP278 (deposited as NRRL B-50634), Bacillus amyloliquefaciens 1013 (deposited as NRRL B-50509), Bacillus amyloliquefaciens 918 (deposited as NRRL B-50508), Bacillus amyloliquefaciens 22CP1 (deposited as ATCC PTA-6508), and Bacillus amyloliquefaciens BS18 (deposited as NRRL B-50633), Bacillus cereus I- 1562, Bacillus firmus 1-1582, Bacillus lichenformis BA842 (deposited as NRRL B-50516), Bacillus lichenformis BL21 (deposited as as NRRL B-50134), Bacillus mycoides NRRL B-21664, Bacillus pumilus NRRL B 21662, Bacillus pumilus NRRL B-30087, Bacillus pumilus ATCC 55608, Bacillus pumilus ATCC 55609, Bacillus pumilus GB34, Bacillus pumilus KFP9F, Bacillus pumilus QST 2808, Bacillus subtilis ATCC 55078, Bacillus subtilis ATCC 55079, Bacillus subtilis MBI 600, Bacillus subtilis NRRL B-21661, Bacillus subtilis NRRL B-21665, Bacillus subtilis CX-9060, Bacillus subtilis GB03, Bacillus subtilis GB07, Bacillus subtilis QST-713, Bacillus subtilis FZB24, Bacillus subtilis D747, Bacillus subtilis 3BP5 (deposited as NRRL B-50510), Bacillus thuringiensis ATCC 13367, Bacillus thuringiensis GC-91, Bacillus thuringiensis NRRL B-21619, Bacillus thuringiensis ABTS-1857, Bacillus thuringiensis SAN 401 I, Bacillus thuringiensis ABG-6305, Bacillus thuringiensis ABG-6346, Bacillus thuringiensis AM65-52, Bacillus thuringiensis SA-12, Bacillus thuringiensis SB4, Bacillus thuringiensis ABTS-351, Bacillus thuringiensis HD-1, Bacillus thuringiensis EG 2348, Bacillus thuringiensis EG 7826, Bacillus thuringiensis EG 7841, Bacillus thuringiensis DSM 2803, Bacillus thuringiensis NB-125, Bacillus thuringiensis NB-176, BRADY, Pseudomonas jessenii PS06, Rhizobium leguminosarum SO12A-2 (IDAC 080305-01), Sinorhizobium frediihizobium CCBAU1 USDA 205, Yersinia entomophaga O82KB8 and combinations thereof, as well as microorganisms that are at least 75, 80, 85, 90, 95, 96, 97, 97.5, 98, 98.5, 99, 99.5 , 99.6, 99.7, 99.8, 99.9% or more identical to any of the aforementioned strains based on 16S rDNA sequence identity.
[0073] In some embodiments, the inoculant compositions of the present disclosure comprise one or more fungi. Non-limiting examples of fungi that may be included in the compositions of the present disclosure include Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices RTI-801, Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma atroviride CNCM 1-1237, Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445, Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum T-3 -39, Trichoderma harzianum ICC012, Trichoderma reesi ATCC 28217, Trichoderma virens ATCC 58678, Trichoderma virens Gl-3, Trichoderma virens GL-21, Trichoderma virens G-41, Trichoderma viridae ATCC 52440, Trichoderma viridae ICC080, Trichoderma viridae TV1 and combinations of these themselves, as well as microorganisms that are at least 75, 80, 85, 90, 95, 96, 97, 97.5, 98, 98.5, 99, 99.5, 99.6, 99.7, 99 .8, 99.9% or more i identical to any of the aforementioned strains based on sequence identity of internal transcribed spacer (ITS) and / or cytochrome c oxidase (CO1).
[0074] In some embodiments, the inoculant compositions of present disclosure comprise one or more mycorrhizal fungi (e.g., one or more endomycorrhizal fungi and / or one or more ectomycorrhizal fungi). Non-limiting examples of mycorrhizal fungi that may be included in the compositions of the present disclosure include mycorrhizal strains such as Gigaspora margarita, Glomus aggregatum, Glomus brasilianum, Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices, Glomus monosporum, Glomus mosseae, Laccaria bicolor , Laccaria laccata, Paraglomus brazilianum, Pisolithus tinctorius, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon luteolus, Rhizopogon villosuli, Scleroderma cepa and Scleroderma citrinum and combinations thereof.
[0075] In some embodiments, the inoculant compositions of the present disclosure comprise one or more diazotrophs and / or phosphate solubilizing microorganisms.
[0076] In some embodiments, the inoculant compositions of the present disclosure comprise one or more biofungicides, bioherbicides, bioinsecticides and / or bionematicides. See generally Burges, Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments (Springer Science & Business Media) (2012); Hall & Menn, Biopesticides: Use and Delivery (Humana Press) (1998); McCoy, et al., Entomogenous fungi, in CRC Handbook of Natural Pesticides. Microbial Pesticides, Part A. Entomogenous Protozoa and Fungi (C.M. Inoffo, ed.), Volume 5:151 to 236 (1988); Samson, et al., Atlas of Entomopathogenic Fungi (Springer-Verlag, Berlin) (1988); and de Faria and Wraight, My coinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types, Biol. Control (2007), doi:10.1016 / j.biocontrol.2007.08.001.
[0077] In some embodiments, the inoculant compositions of the present disclosure comprise one or more modified microbial strains.
[0078] The compositions of the present invention may comprise vegetative cells and / or dormant spores. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more of the microbes in a composition of the present disclosure are present as vegetative cells. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more of the microbes in a composition of the present disclosure are present as spores. In some embodiments, the inoculant compositions of the present disclosure are devoid of vegetative cells. In some embodiments, the inoculant compositions of the present disclosure are devoid of spores.
[0079] Microbial spores may be produced by any suitable method (or methods), including, but not limited to, liquid fermentation and solid-state fermentation. See, for example, Cunningham et al., Can. J.Bot. 68:2270 (1990); Friesen et al., Appl. Microbiol. Biotech. 68:397 (2005).
[0080] Spores may be harvested and / or concentrated using any suitable method (or methods), including, but not limited to, centrifugation (e.g., density gradient centrifugation, disk centrifugation, tube centrifugation) , coagulation, decantation, felt bed collection, filtration (eg drum filtration, sieving, ultrafiltration), flocculation, impaction and capture (eg cyclone spore capture, liquid entrapment).
[0081] Microorganisms can be incorporated into the inoculant compositions of the present disclosure in any suitable amount (or amounts) / concentration (or compositions). The absolute value of the amount / concentration of microbial cells / spores sufficient to cause a desired effect can be affected by factors such as the type of effect desired; the magnitude of the desired effect; the type, size and volume of material to which the composition will be applied; the type of microbial cells / spores; the inherent stability of microbial cells / spores; and storage conditions (eg, temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount / concentration using routine dose-response experiments after reading the present disclosure. Guidance for selecting appropriate amounts / concentrations can be found, for example, in International Patent Applications Nos. PCT / US2016 / 050529 and PCT / US2016 / 050647 and US Provisional Patent Applications 62 / 296,798; 62 / 271,857; 62 / 347,773; 62 / 343,217; 62 / 296,784; 62 / 271,873; 62 / 347,785; 62 / 347,794; and 62 / 347,805.
[0082] In some embodiments, the microbial cells / spores comprise from about 0.1 to about 50% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 , 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5 , 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27 , 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50% or more ( by weight) of one or more microorganisms. In some embodiments, the amount / concentration of microbial cells / spores is about 1, 2, 3, 4, or 5 to about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25% (by weight) of the inoculant composition.
[0083] In some embodiments, the microbial cells / spores are present in an amount in the range of about 1 x 10 1 at about 1 x IO 20 colony forming units (cfu) per gram and / or milliliter of inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 or more microbial cells / spores per gram and / or milliliter of composition inoculant.
[0084] In some embodiments, the amount / concentration of microbial cells / spores is that amount / concentration that is effective to enhance germination and / or emergence of plant seeds to which the inoculant composition is applied.
[0085] In some embodiments, the amount / concentration of microbial cells / spores is that amount / concentration that is effective to enhance germination and / or emergence of plant seeds introduced into a plant growth medium (e.g., soil) treated with the inoculant composition.
[0086] In some embodiments, the amount / concentration of microbial cells / spores is that amount / concentration that is effective to enhance the growth and / or yield of the plants and plant parts to which the inoculant composition is applied.
[0087] In some embodiments, the amount / concentration of microbial cells / spores is that amount / concentration that is effective to enhance the growth and / or yield of plants and plant parts grown in a plant growth medium (e.g., soil) treated with the inoculant composition.
[0088] In some embodiments, the amount / concentration of microbial cells / spores is that amount / concentration that is effective to fix atmospheric nitrogen, solubilize phosphate, and / or control one or more plant pathogenic pests when the inoculant composition is applied to a plant or plant part.
[0089] In some embodiments, the amount / concentration of microbial cells / spores is that amount / concentration that is effective for fixing atmospheric nitrogen, solubilizing phosphate, and / or controlling one or more plant pathogenic pests when the inoculant composition is introduced into a plant growth medium (eg soil).
[0090] It is to be understood that microbial cells / spores may be incorporated into inoculant compositions of the present disclosure in any suitable manner, including, but not limited to, powders and granules comprising vegetative cells and / or spores (e.g., liquid cultures that have been drum dried, evaporative dried, fluidized bed dried, freeze dried, spray dried, spray freeze dried, tray dried and / or vacuum dried to produce powders / granules).
[0091] The inoculant compositions of the present disclosure may comprise any suitable carrier (or carriers), including, but not limited to, foliar compatible carriers, seed compatible carriers, and soil compatible carriers. Selection of appropriate carrier components will depend on the intended applications and the microorganisms present in the inoculant composition.
[0092] In some embodiments, the carrier essentially consists of one or more oils and / or paraffin waxes.
[0093] The inoculant compositions of the present disclosure may comprise any suitable paraffin oil and / or wax (or suitable paraffin oils and / or waxes), including, but not limited to, CAS Number 8002-74-2, CAS Number 8009- 03-8, CAS number 8012-95-1, CAS number 63231-60-7, CAS number 64742-43-4, CAS number 64742-56-9, CAS number 64742-61-6 and 64742-65-0. Non-limiting examples of paraffin oils and waxes that can be used in the compositions and methods of the present disclosure include PARRAFINIC OIL 98.5EC (Vioryl S.A., Afidnes, Greece), SUN AG® oils (e.g., 7N and 1 IN; HollyFrontier Refining & Marketing LLC, Tulsa, OK), SUNSPRAY® oils (e.g., 6E, 6N, 8N, 1 IN, MLO, ULTRA-FINE®; HollyFrontier Refining & Marketing LLC, Tulsa, OK) and SUNWAX® waxes (e.g., 130, DM-160, DP-116, DS-80, DS 116, DS127, DS137, DS155, LP-127, LP-130, LP-140, LP-155, SP,137, SW-137; HollyFrontier Refining & Marketing LLC, Tulsa, OK).
[0094] In some embodiments, the paraffin oil and / or wax (or paraffin oils and / or waxes) comprises about / at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 , 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5% or more (by weight) of the carrier. For example, in some embodiments, dodecane comprises about / at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5% or more (by weight) of the carrier.
[0095] In some embodiments, methyl soiate comprises about / at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5% or more (by weight) of the inoculant composition. For example, in some embodiments, the paraffin oil and / or wax (or paraffin oils and / or waxes) comprises about / at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 , 60, 65, 70, 75, 80, 85, 90, 91,92,93,94,95,96,97,98.99% or more (by weight) of the inoculant composition.
[0096] In some embodiments, the inoculant compositions of the present disclosure comprise one or more paraffin oils and / or waxes in an amount / concentration sufficient to ensure that microbial cells / spores remain viable therein after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 , 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92 , 96, 100, 104 weeks or more; cryopreservation at or below -80 °C for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or plus and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[0097] In some embodiments, the inoculant compositions of The present disclosure comprises one or more paraffin oils and / or waxes in an amount / concentration sufficient to ensure that at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of microbial cells / spores remain viable after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below -80 °C for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 , 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally foliar application and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or greater and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[0098] In some embodiments, the inoculant compositions of the present disclosure comprise one or more paraffin oils and / or waxes in an amount / concentration sufficient to ensure that at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 or more colony forming units of microbial cells / spores remain viable per gram and / or milliliter of inoculant composition after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below -80 °C for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or plus and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[0099] In some embodiments, the carrier comprises one or more carrier components in addition to the paraffin oil and / or wax (or paraffin oils and / or waxes). For example, the inoculant compositions of the present disclosure can comprise one or more solid carrier components (e.g., one or more powders and / or granules) and / or liquid carriers (e.g., one or more aqueous solvents), in addition to oil. and / or paraffin wax (or paraffin oils and / or waxes).
[00100] Non-limiting examples of solid carrier components that can be included in compositions of the present disclosure include clays (e.g., attapulgite clays, montmorillonite clay, etc.), peat-based powders and granules, freeze-dried powders, spray dried, freeze and spray dried powders and combinations thereof.
[00101] Non-limiting examples of liquid / gel carrier components that may be included in the compositions of the present disclosure include acetone, n-alkylpyrrolidones (e.g., AGSOLEX™ wetting agents; Ashland, Inc., Covington, KY), cycloparaffinic hydrocarbons (e.g., NAPPAR™ 6; ExxonMobil Chemical Company, Spring, TX), decane, dichloromethane, ethanol, ethoxylated alcohols (e.g., TOMADOL® (Air Products and Chemicals, Inc., Allentown, PA), ethyl lactate, hexane , hexylether, isoparaffinic hydrocarbons (eg, ISOPAR™, ISOPAR™ L, ISOPAR™ M, ISOPAR™ V; ExxonMobil Chemical Company, Spring, TX), isopropanol, methanol, methyl soiate / ethyl lactate co-solvent blends (e.g. (e.g., STEPOSOL™, Stepan), mineral turpentine (e.g., VARSOL™; ExxonMobil Chemical Company, Spring, TX), nonane, oils (e.g., mineral oil, olive oil, peanut oil, soybean oil, sunflower, tung oil), pentadecane, pet-based oils oil (for example, AROMATIC™ and SOLVESSO™ fluids; ExxonMobil Chemical Company, Spring, TX), polyethylene glycols (e.g., PEG 200, PEG 300, PEG 400, etc.), polysorbates (e.g., polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, etc.), propan-2-ol, 1,2-propanediol , propylene glycols (eg, PPG-9, PPG-10, PPG-17, PPG-20, PPG-26, etc.), silicones (siloxanes, trisiloxanes, etc.), TERGITOL™ 15-S surfactants, such as TERGITOL ™ 15-S-9 (The Dow Chemical Company, Midland, MI), etc.), trichlorethylene and combinations thereof.
[00102] Additional examples of carrier components can be found in BURGES, FORMULATION OF MICROBIAL BIOPESTICIDES: BENEFICIAL MICROORGANISMS, NEMATODES AND SEED TREATMENTS (Springer Science & Business Media) (2012); Inoue & Horikoshi, J. FERMENTATIONBIOENG. 71(3):194 (1991).
[00103] Additional carrier components can be incorporated into inoculant compositions of the present disclosure in any suitable amount (or amounts) / concentration (or concentrations). The absolute value of the amount / concentration that is sufficient to cause the desired effect (or effects) can be affected by factors such as the type, size and volume of material to which the composition will be applied, the type (or types) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition, and storage conditions (eg, temperature, relative humidity, duration). Those skilled in the art will understand how to select effective amounts / concentrations using routine dose-response experiments. Guidance for selecting appropriate amounts / concentrations can be found, for example, in International Patent Applications Nos. PCT / US2016 / 050529 and PCT / US2016 / 050647 and US Provisional Patent Applications 62 / 296,798; 62 / 271,857; 62 / 347,773; 62 / 343,217; 62 / 296,784; 62 / 271,873; 62 / 347,785; 62 / 347,794; and 62 / 347,805.
[00104] In some embodiments, the inoculant compositions of The present disclosure comprises one or more additional carrier components in an amount / concentration of from about 1 to about 99% or greater (by weight, based on the total weight of the inoculant composition). For example, the inoculant compositions of the present disclosure can comprise about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 , 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% (by weight) of one or more solid carrier components.
[00105] Inoculating compositions of the present disclosure may comprise any suitable stabilizing compound (or compounds), including, but not limited to, maltodextrins, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, humic acids, fulvic acids, malt extracts, peat extracts , betaines, prolines, sarcosines, peptones, skimmed milk, oxidation control components, hygroscopic polymers and UV protectors.
[00106] In some embodiments, the inoculant composition comprises one or more maltodextrins (e.g., one or more maltodextrins having a dextrose equivalent value (DEV) of about 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25). According to some embodiments, an inoculant composition comprises one or more maltodextrins having a DEV of from about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11, 12, 14, 15, 16, 17, 18, 19 or 20, or about 15 to about 16, 17, 18, 19 or 20. According to some embodiments, the inoculant composition comprises a combination of maltodextrins having a DEV of from about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11, 12, 14, 15, 16, 17, 18, 19 or 20, or about 15 to about 16, 17, 18, 19 or 20. Non-limiting examples of maltodextrins include MALTRIN® M040 (DEV = 5; molecular weight = 3600; Grain Processing Corporation, Muscatine, IA), MALTRIN® M100 (DEV = 10; molecular weight = 1800; Grain Processing Corporation, Muscatine, IA), MALTRIN® Ml50 (DEV = 15; molecular weight = 1200; Grain Processing Corporation, Muscatine, IA), MALTRIN® Ml80 (DEV = 18; molecular weight = 1050; Grain Processing Corporation, Muscatine, IA) , MALTRIN® M200 (DEV = 20; molecular weight = 900; Grain Processing Corporation, Muscatine, IA), MALTRIN® M250 (DEV = 25; molecular weight = 720; Grain Processing Corporation, Muscatine, IA); MALTRIN QD® M580 (DEV = 16.5-19.9; Grain Processing Corporation, Muscatine, IA); MALTRIN QD® M585 (DEV = 15.0-19.9; Grain Processing Corporation, Muscatine, IA); MALTRIN QD® M600 (DEV = 20.0-23.0; Grain Processing Corporation, Muscatine, IA); GLOBE® Plus 15 DE (Ingredion Inc., Westchester, IL); and their combinations.
[00107] In some embodiments, the inoculant composition comprises one or more monosaccharides (for example, allose, altrose, arabinose, fructose, galactose, glucose, glutose, iodose, lyxose, mannose, ribose, thalose, threose and / or xylose). According to some embodiments, the inoculant composition comprises glucose. According to some embodiments, the inoculant composition does not comprise glucose.
[00108] In some embodiments, the inoculant composition comprises one or more disaccharides (for example, cellobiose, chitobiose, gentiobiose, gentiobiulose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose (for example, maltose monohydrate, anhydrous maltose ), maltulose, mannobiose, melibiose, melibiulose, nigerosis, palatinose, rutinose, rutinulose, sophorose, sucrose, trehalose, turanose and / or xylobiose). According to some embodiments, the inoculant composition comprises maltose. According to some embodiments, the inoculant composition does not comprise maltose. According to some embodiments, the inoculant composition comprises trehalose. According to some embodiments, the inoculant composition does not comprise trehalose.
[00109] In some embodiments, the inoculant composition comprises one or more oligosaccharides (e.g. fructo-oligosaccharides, galacto-oligosaccharides, mannon-oligosaccharides and / or raffinose).
[00110] In some embodiments, the inoculant composition comprises one or more sugar alcohols (for example, arabitol, erythrol, fucitol, galactitol, glycerol, iditol, inositol, isomalt, lactitol, maltitol, maltotetraitol, maltotriitol, mannitol, polyglycitol , ribitol, sorbitol, threitol, volemitol and / or xylitol).
[00111] In some embodiments, the inoculant composition comprises one or more humic acids (e.g., one or more leonardite humic acids, lignite humic acids, peat humic acids, and water-extracted humic acids). In some embodiments, the inoculant composition comprises ammonium humate, boron humate, potassium humate and / or sodium humate. In some embodiments, one or more of ammonium humate, boron humate, potassium humate and sodium humate is / are excluded from the inoculant composition. Non-limiting examples of humic acids that may be useful in the embodiments of the present disclosure include MDL number MFCD00147177 (CAS number 1415-93-6), MDL number MFCD00135560 (CAS number 68131-04-4), MDL number MFCS22495372 (CAS number 68514- 28-3), CAS Number 93924-35-7 and CAS Number 308067-45-0.
[00112] In some embodiments, the inoculant composition comprises one or more fulvic acids (e.g., one or more fulvic acids from leonardite, fulvic acids from lignite, fulvic acids from peat, and / or fulvic acids extracted from water). In some embodiments, the inoculant composition comprises ammonium fulvate, boron fulvate, potassium fulvate and / or sodium fulvate. In some embodiments, one or more of ammonium fulvate, boron fulvate, potassium fulvate and sodium fulvate is / are excluded from the inoculant compositions of the present disclosure. Non-limiting examples of fulvic acids that may be useful in the embodiments of the present disclosure include MDL number MFCD09838488 (CAS number 479-66-3).
[00113] In some embodiments, the inoculant composition comprises one or more betaines (e.g. trimethylglycine).
[00114] In some embodiments, the inoculant composition comprises one or more peptones (e.g. bacterial peptones, meat peptones, milk peptones, vegetable peptones and yeast peptones).
[00115] In some embodiments, the inoculant composition comprises one or more oxidation control components (e.g., one or more antioxidants and / or oxygen scavengers). According to some embodiments, the inoculant composition comprises one or more oxygen scavengers, such as ascorbic acid, ascorbate salts, catechol and / or sodium hydrogen carbonate. According to some embodiments, the inoculant composition comprises one or more antioxidants, such as ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, carotenoids, lipoic acid, phenolic compounds (e.g., flavonoids, flavones, flavonols), ascorbate potassium, sodium ascorbate, thiols (eg glutathione, lipoic acid, N-acetyl cysteine), tocopherols, tocotrienols, ubiquinone and / or uric acid. Non-limiting examples of antioxidants include those that are soluble in the cell membrane (e.g., alpha tocopherol (vitamin E), ascorbyl palmitate) and those that are soluble in water (e.g., ascorbic acid and isomers or ascorbic acid, sodium salts or potassium of ascorbic acid or isomers or ascorbic acid, glutathione, sodium or potassium salts of glutathione). In some embodiments, the use of a membrane soluble antioxidant necessitates the addition of one or more surfactants to adequately disperse the antioxidant within the inoculant composition. According to some embodiments, the inoculant composition is / comprises ascorbic acid and / or glutathione.
[00116] In some embodiments, the inoculant composition comprises one or more hygroscopic polymers (e.g. hygroscopic agars, albumins, alginates, carrageenans, celluloses, gums (e.g. cellulose gum, guar gum, acacia gum, combrete gum, xanthan gum), methylcellulose, nylons, pectins, acids polyacrylics, polycaprolactones, polycarbonates, polyethylene glycols (PEG), polyethyleneimines (PEI), polylactides, polymethylacrylates (PMA), polyurethanes, polyvinyl alcohols (PVA), polyvinylpyrrolidones (PVP), propylene glycols, sodium carboxymethylcelluloses and / or starches). Non-limiting examples of polymers include AGRIMER™ polymers (e.g. 30, AL-10 LC, AL-22, AT / ATF, VA 3E, VA 31, VA 5E, VA 51, VA 6, VA 6E, VA 7E, VA 71, VEMA AN-216, VEMA AN-990, VEMA AN-1200, VEMA AN-1980, VEMA H-815MS; Ashland Specialty Ingredients, Wilmington, DE), EASYSPERSE™ polymers (Ashland Specialty Ingredients, Wilmington, DE); DISCO™ AG polymers (e.g. L-250, L-280, L-285, L-286, L-320, L-323, L-517, L-519, L-520, L800; Incotec Inc., Salinas, CA), KELZAN® polymers (Bri-Chem Supply Ltd., Calgary, Alberta, CA), SEEDWORX™ polymers (e.g., Bio 200; Aginnovation, LLC, Walnut Groove, CA), TICAXAN® xanthan powders, such such as PRE-HYDRATED® TICAXAN® Rapid-3 Powder (TIC Gums, White Marsh, MD) and combinations thereof. Additional examples of polymers can be found in Pouci, et al. Am. J. Agric. Biol. Sci. 3(1):299 (2008).
[00117] In some embodiments, the inoculant composition comprises one or more UV protectants (e.g., one or more aromatic amino acids (e.g., tryptophan, tyrosine), carotenoids, cinnamates, lignosulfonates (e.g., calcium lignosulfonate, calcium lignosulfonate, sodium), melanins, mycosporins, polyphenols and / or salicylates). Non-limiting examples of UV protectants include Borregaard LignoTech™ lignosulfonates (e.g. Borresperse 3A, Borresperse CA, Borresperse NA, Marasperse AG, Norlig A, Norlig 11 D, Ufoxane 3A, Ultrazine NA, Vanisperse CB; Borregaard Lignotech, Sarpsborg, Norway) and their combinations. Additional examples of UV protectants can be found in Burges, Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments (Springer Science & Business Media) (2012).
[00118] The inoculant compositions of the present disclosure may comprise any suitable biostimulant (or biostimulants), including, but not limited to, seaweed extracts (for example, Ascophyllum nodosum extracts such as alginate, Ecklonia maxima extracts, etc.), myo -inositol, glycine and combinations thereof.
[00119] The inoculant compositions of the present disclosure may comprise any suitable microbial extract, including, without limitation, bacterial extracts, fungal extracts and compositions thereof. In some embodiments, inoculant compositions of the present disclosure comprise one or more extracts of media comprising one or more diazotrophs, phosphate-solubilizing microorganisms, and biopesticides. In some embodiments, the inoculant compositions of the present disclosure comprise an extract of media comprising one or more of the microbial strains included in Appendix A.
[00120] The inoculant compositions of the present disclosure can comprise any suitable nutrient (or nutrients), which includes, without limitation, organic acids (e.g., acetic acid, citric acid, lactic acid, malic acid, taurine, etc.), macrominerals (e.g. phosphorus, calcium, magnesium, potassium, sodium, iron, etc.), trace minerals (e.g. boron, cobalt, chloride, chromium, copper, fluoride, iodine, iron, manganese, molybdenum, selenium, zinc, etc.), vitamins, (e.g. vitamin A, vitamin B complex (i.e. vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin Bg, vitamin B9, vitamin B12, choline) vitamin C, vitamin D, vitamin E, vitamin K, carotenoids (a- carotene, P-carotene, cryptoxanthin, lutein, lycopene, zeaxanthia, etc.) and combinations thereof. In some embodiments, the inoculant compositions of the present disclosure comprise phosphorus, boron, chlorine, iron, manganese, molybdenum and / or zinc.
[00121] The inoculant compositions of the present disclosure may comprise any suitable pest attractant (or attractants) and / or feeding stimulant (or stimulants), including, but not limited to, brevicomine, ceralure, codlelure, cuelure, disparlure, dominicalure, eugenol , frontalin, gossiplure, grandlure, hexalure, ipsdienol, ipsenol, japonilure, latitlure, lineatin, litlure, looplure, medlure, megatomic acid, methyl eugenol, moguchun, a-multistriatin, muscalure, orphalure, orictalure, ostramone, rescalure, siglure, sulcatol , trimedlure and / or trunc-call.
[00122] Inoculum compositions of the present disclosure may comprise any suitable pesticide (or pesticides), including, without limitation, acaricides, fungicides, herbicides, insecticides and nematicides.
[00123] Fungicides can be selected to provide effective control against a broad spectrum of phytopathogenic fungi (and fungal-like organisms), including, but not limited to, soil-borne fungi of the classes Ascomycetes, Basidiomycetes, Chytridiomycetes, Deuteromycetes (syn. . Fungi imperfectí), Peronosporomycetes (syn. Oomycetes), Plasmodiophoromycetes and Zygomycetes. According to some embodiments, the inoculant composition comprises a fungicide (or combination of fungicides) that is toxic to one or more strains among Albugo (e.g., A. Candida), Alternaria (e.g. s A. alternata), Aspergillus (eg, A. candidus, A. clavatus, A. flavus, A. fumigatus, A. parasiticus, A. restrictus, A. Soyae, A. solani), Blumeria (eg ., B. graminis), Botrytis (eg, B. cinerea), Cladosporum (eg, C. cladosporioides), Colletotrichum (eg, C. acutatum, C. boninense, C. capsici, C. caudatum, C. coccodes, C. crassipes, C. dematium, C. destructivum, C.fragariae, C. gloeosporioides, C. graminicola, C. kehawee, C. lindemuthianum, C. musae, C. orbiculare, C. spinaceae, C. sublineolum, C. trifolii, C. truncatum), Fusarium (eg, F. graminearum, F. moniliforme, F. oxysporum, F. roseum, F. tricinctum), Helminthosporium, Magnaporthe (eg, M. grisea, M. oryzae), Melamspora (eg, M. Uni), Mycosphaerella (eg, M. graminicola), Nematospora , Penicillium (eg, P. rugulosum, P. verrucosum), Phakopsora (eg, P. pachyrhizi), Phomopsis, Phytiphtoria (eg, P. infestans), Puccinia (eg, P. graminis, P. striiformis, P. tritici, P. triticina), Pucivinia (eg, P. graministice), Pythium, Pytophthora, Rhizoctonia (eg, R. solani), Scopulariopsis, Selerotinia, Thielaviopsis and / or Ustilago (eg ^t / . maydis). Additional examples of fungi can be found in Bradley, Managing Diseases, in Illinois Agronomy Handbook (2008).
[00124] Herbicides can be selected to provide effective control against a broad spectrum of plants, including, but not limited to, plants in the Asteraceae, Caryophyllaceae, Poaceae, and Poligonaceae families. According to some embodiments, the inoculant composition comprises a herbicide (or combination of herbicides) that is toxic to one or more strains among Echinochloa (e.g., E. brevipedicellata, E. callopus, E. chacoensis, E. colona, E. crus-galli, E. crus-pavonis, E. elliptica, E. esculenta, E. frumentacea, E. glabrescens, E. haploclada, E. helodes, E. holciformis, E. inundata, E. jaliscana, E. Jubata, E. kimberleyensis, E. lacunaria, E. macrandra, E. muricata, E. obtusiflora, E. oplismenoides, E. orzyoides, E. paludigena, E. picta, E. pithopus, E. polystachya, E. praestans, E. pyramidalis, E. rotundiflora, E. stagnina, E. telmatophila, E. turneriana, E. ugandensis, E. walteri), Fallopia (eg, F. baldschuanica, F. japonica, F. sachalinensis), Stellaria (e.g., S. media) and / or Taraxacum (e.g., T. albidum, T. aphrogenes, T. brevicorniculatum, T. californicum, T. centrasiatum, T. ceratophorum, T. erythrospermum, T. farinosum, T. holmboei, T. japonicum, T. kok-saghyz, T. laevigatum T. officinale, T. platycarpum). Additional plant species that can be targeted by inoculant compositions of the present disclosure can be found in Hager, Weed Management, in Illinois Agronomy Handbook (2008) and Loux et al., Weed Control Guide for Ohio, Indiana and Illinois (2015).
[00125] Insecticides can be selected to provide effective control against a broad spectrum of insects, including, but not limited to, insects of the orders Coleoptera, Dermaptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Lepidoptera, Orthoptera and Thysanoptera. For example, the inoculant compositions of the present disclosure may comprise one or more insecticides toxic to insects from the families Acrididae, Aleytodidae, Anobiidae, Anthomyiidae, Aphididae, Bostrichidae, Bruchidae, Cecidomyiidae, Cerambycidae, Cercopidae, Chrysomelidae, Cicadellidae, Coccinellidae, Cryllotalpidae, Cucujidae , Curculionidae, Dermestidae, Elateridae, Gelechiidae, Lygaeidae, Meloidae, Membracidae, Miridae, Noctuidae, Pentatomidae, Pyralidae, Scarabaeidae, Silvanidae, Spingidae, Tenebrionidae and / or Thripidae. According to some embodiments, the inoculant composition comprises an insecticide (or combination of insecticides) that is toxic to one or more species among Acalymma, Acanthaoscelides (e.g., A. obtectus, ), Anasa (e.g., A. . tristis), Anastrepha (eg, A. ludens), Anoplophora (eg, A. glabripennis), Anthonomus (eg, A. eugenii), Acyrthosiphon (eg, A. pisum ), Bactrocera (eg. s B. dosalis), Bemisia (eg, B. argentifolii, B. tabací), Brevicoryne (eg, B. brassicae), Bruchidius (eg, B. atrolineatus), Bruchus (eg, B. ., B. atomarius, B. dentipes, B. lends, B. pisorum, and / or B. rufipes), Callosobruchus (eg, C. chinensis, C. maculatus, C. rhodesianus, C. subinnotatus, C. theobromae), Caryedon (eg, C. serratus), Cassadinae, Ceratitis (eg, C. capitata), Chrysomelinae, Circulifer (eg, C. tenellus\ Criocerinae, Cryptocephalinae, Cryptolestes (eg. eg, C. ferrugineus, C. pusillis, C. pussilloides), Cylas (eg, C. fonnicarius), Delia (eg, D. antiqua), Diabrotica, Diaphania (eg, D. . nitidalis\ Diaphorina (eg, D. citrí), Donaciinae, Ephestia (e.g. E. cautella, E. elutella, E. keuhniella), Epilachna (e.g. E. varivestris), Epiphyas (e.g. E. postvittana), Eumolpinae, Galerucinae, Helicoverpa ( eg H. zed), Heteroligus (eg H. meles), lobesia (eg I. botrand), Lamprosomatinae, Lasioderma (eg L. serricorne), Leptinotarsa ( eg L. decemlineata), Leptoglossus, Liriomyza (eg L. trifolii), Manducca, Melittia (eg M. cucurbitae), Myzus (eg M. persicae), Nezara (eg, N. viridula), Orzaephilus (eg, O. merator, O. surinamensis), Ostrinia (eg, O. nubilalis), Phthorimaea (eg, P. operculella ), Pieris (eg P. rapae), Plodia (eg P. interpunctella), Plutella (eg P. xylostella), Popillia (eg P. japonica), Prostephanus (eg, P. truncates), Psila, Rhizopertha (eg, R. dominica), Rhopalosiphum (eg, R. maidis), Sagrinae, Solenopsis (eg, S. Invicta ), Spilopyrinae, Sitophilus (eg, S. granaries, S. oryzae and / or S. zeamais), Sitotroga (eg, S. cerealella), Spod optera (eg, S. frugiperda), Stegobium (eg, S. paniceum), Synetinae, Tenebrio (eg, T. malens and / or T. molitor), Thrips (eg, T. molitor). , T. tabaci), Trialeurodes (eg, T. vaporariorum), Tribolium (eg, T. castaneume and / or T. confusum), Trichoplusia (eg, T. ni), Trogoderma (eg, T. ni). .eg T. granarium) and Trogossitidae (eg T. mauritanicus). Additional insect species that can be targeted by inoculant compositions of the present disclosure can be found in Capinera, Handbook of Vegetable Pests (2001) and Steffey and Gray, Managing Insect Pests, in Illinois Agronomy Handbook (2008).
[00126] Nematicides can be selected to provide effective control against a broad spectrum of nematodes, including, but not limited to, phytoparasitic nematodes of the Chromadorea and Enoplea classes. According to some embodiments, the inoculant composition comprises a nematicide (or combination of nematicides) that is toxic to one or more strains among Anguina, Aphelenchoides, Belonolaimus, Bursaphelenchus, Ditylenchus, Globodera, Helicotylenchus, Heterodera, Hirschmanniella, Meloidogyne, Naccobus, Pratylenchus , Radopholus, Rotylenshulus, Trichodorus, Tylenchulus and / or Xiphinema. Additional examples that can be targeted by inoculant compositions of the present disclosure can be found in Capinera, Handbook of Vegetable Pests (2001) and Niblack, Nematodes, in Illinois Agronomy Handbook (2008).
[00127] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chemical fungicides. Examples non-limiting chemical fungicides include strobilurins such as azoxystrobin, coumetoxystrobin, coumoxystrobin, dimoxystrobin, estroburin, fluoxastrobin, kresoxim-methyl, metominostrobin, orissastrobin, picoxystrobin, pyraclostrobin, pyramethostrobin, pyraoxystrobin, piribencarb, trifloxystrobin, 2-[2-(2,5-dimethyl-phenoxymethyl)-phenyl]-3-methoxy-acrylic acid methyl ester and 2-(2-(3-(2,6-dichlorophenyl)- l- methyl-allylideneamino-oxymethyl)-phenyl)-2-methoxy-imino-N-methyl-acetamide; carboxamides, such as carboxanilides (e.g., benalaxyl, benalaxyl-M, benodanil, bixaphene, boscalid, carboxin, fenfuram, fenhexamide, flutolanil, fluxapyroxad, furamethpyr, isopyrazam, isothianil, chiralaxyl, mepronil, metalaxyl, metalaxyl-M (mefenoxam), ofurace, oxadixyl, oxycarboxin, penflufen, penthiopyrad, sedaxane, tecloftalam, tifluzamide, tiadinyl, 2-amino-4-methyl-thiazole-5 -carboxanilide, N-(4'-trifluoromethylthiobiphenyl-2-yl)-3- difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(2-(1,3,3-trimethylbutyl)-phenyl)-1,3-dimethyl-5-fluoro-1H-pyrazole-4-carboxamide) , morpholid carboxylic acids (eg dimethomorph, flumorph, pyrimorph), benzoic acid amides (eg flumetover, fluopicolide, fluopyram, zoxamide), carpropamide, dicyclomet, mandiproamide, oxytetracycline, silthiopham and N-(6-methoxy-pyridin) acid amide -3-yl)carboxylic cyclopropane; azoles, such as triazoles (e.g., azaconazole, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibeconazole, ipconazole, metconazole, myclobutanil, oxpoconazole, paclobutrazol, penconazole, propiconazole, prothioconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole) and imidazoles (e.g. cyazofamid, imazalil, pefurazoate, prochloraz, triflumizol); heterocyclic compounds such as pyridines (eg fluazinam, pyrifenox (cf.Dlb), 3-[5-(4-chloro-phenyl)-2,3-dimethyl-isoxazolidin-3-yl]-pyridine, 3-[5 -(4-methyl-phenyl)-2,3-dimethyl-isoxazolidin-3-yl]-pyridine), pyrimidines (eg, bupirimate, cyprodinil, diflumetorim, fenarimol, ferimzone, mepanipyrim, nitrapyrin, nuarimol, pyrimethanil), piperazines (e.g. triforine), pyrroles (e.g. fenpiclonil, fludioxonil), morpheolines (e.g. aldimorph, dodemorph, dodemorph-acetate, fenpropimorph, tridemorph), piperidines (e.g. fenpropidin), dicarboximides (e.g. fluoroimide, iprodione, procymidone, vinclozolin), non-aromatic 5-membered heterocycles (e.g. famoxadone, fenamidone, flutianil, octilinone, probenazole, 5-amino-2-isopropyl-3-oxo-4-ortho-tolyl acid S-allyl ester -2,3-dihydro-pyrazol-1-carbothioic acid), acibenzolar-S-methyl, ametoctradine, amisulbrom, anilazine, blasticidin-S, captafol, captan, quinomethionate, dazomet, debacarb, diclomezine, diphenzoquat, diphenzoquat-methylsulfate, fenoxanil, Folpet, oxolinic acid, piperaline, proquinazid, pyroquilone, quinoxyfen, triazoxide, tricyclazole, 2-butoxy-6-iodo-3-propylchromen-4-one, 5-chloro-1-( 4,6-dimethoxy-pyrimidin-2-yl)-2-methyl-1H-benzoimidazole and 5-chloro-7-(4-methylpiperidin-1-yl)-6-(2,4,6-trifluorophenyl)-[ 1,2,4]triazolo-[1,5-a]pyrimidine; benzimidazoles, such as carbendazim; and other active substances such as guanidines (eg guanidine, dodine, dodine free base, guazatine, guazatine-acetate, iminoctadine), iminoctadine-triacetate, and iminoctadine-tris(albesylate); antibiotics (e.g. kasugamycin, kasugamycin hydrochloride hydrate, streptomycin, polyoxin and validamycin A); nitrophenyl derivatives (e.g. binapacryl, dichlorane, dinobutom, dinocap, nitrothalisopropyl, technazem); organometallic compounds (for example, fentin salts, such as fentin acetate, fentin chloride, fentin hydroxide); compounds of sulfur-containing heterocyclyls (eg, ditianom, isoprothiolane); organophosphorus compounds (for example edifenphos, fosetyl, fosetyl aluminum, iprobenphos, phosphorous acid and its salts, pyrazophos, tolclofos methyl); organochlorine compounds (eg, chlorothalonil, dichlofluanid, dichlorofen, flusulfamide, hexachlorobenzene, pencycuron, pentachlorphenol and its salts, phthalide, quintazene, thiophanate-methyl, thiophanate, tolylfluanid, N-(4-chloro-2-nitro-phenyl)- N-ethyl-4-methyl-benzenesulfonamide) and inorganic active substances (eg Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur) and combinations thereof. In some embodiments, the inoculant compositions of the present disclosure comprise acibenzolar-S-methyl, azoxystrobin, benalaxyl, bixaphene, boscalid, carbendazim, cyproconazole, dimethomorph, epoxiconazole, fludioxonil, fluopyram, fluoxastrobin, flutianil, flutolanil, fluxapyroxad, fosetyl-Al, ipconazole , isopyrazam, kresoxim-methyl, mefenoxam, metalaxyl, metconazole, myclobutanil, orysastrobin, penflufen, penthiopyrad, picoxystrobin, propiconazole, prothioconazole, pyraclostrobin, sedaxane, silthiofam, tebuconazole, thiabendazole, tifluzamide, thiophanate, tolclofos-methyl, trifloxystrobin and triticonazole. In some embodiments, the inoculant compositions of the present disclosure comprise azoxystrobin, pyraclostrobin, fluoxastrobin, trifloxystrobin, ipconazole, prothioconazole, sedaxan, fludioxonil, metalaxyl, mefenoxam, thiabendazole, fluxapyroxad and / or fluopyram. In some embodiments, the inoculant compositions of the present disclosure comprise one or more aromatic hydrocarbons, benzimidazoles, benzthiadiazole, carboxamides, carboxylic acid amides, morpholines, phenylamides, phosphonates, external quinone inhibitors (e.g., strobilurins), thiazolidines, thiophanates, thiophene carboxamides and / or triazoles.
[00128] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chemical herbicides. Examples non-limiting chemical herbicides include 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), ametrine, amicarbazone, aminocyclopyrachlor, acetochlor, acifluorfen, alachlor, atrazine, azafenidin, bentazone, benzofenap, bifenox, bromacyl, bromoxynil, butachlor, butafenacyl, butroxidim, carfentrazone-ethyl, chlorimurone, chlorotoluure, clethodim, clodinafop, clomazone, cyanazine, cycloxydim, cyhalofop, desmedipham, desmetrine, dicamba, diclofop, dimefurone, diuron, dithiopyr, fenoxaprop, fluazifop, fluazifop-P, fluometurone, flufenpyr-ethyl, flumiclorac-pentyl, flumioxazine, fluoroglycofeon, fluthiacet-methyl, fomesafe, fomesafen, glyphosate, glufosinate, haloxyfop, hexazinone, imazamox, imazaquin, imazethapyr, ioxynyl, isoproturone, isoxaflutol, lactofen, linuron, mecoprop, mecoprop-P, mesotrione, metamitrone, methazochlor, methybenzuron, metolachlor (and S-metolachlor), methoxuron, metribuzin, monolinuron, oxadiargyl, oxadiazone, oxyfluorf ene, phenmedipham, pretilachlor, profoxydim, prometone, prometri, propachlor, propanil, propaquizafop, propisochlor, pyraflufeon-ethyl, pyrazone, pyrazolinate, pyrazoxifene, pyridate, quizalofop, quizalofop-P (eg, quizalofop-ethyl, quizalofop-P-ethyl , clodinafop-propargyl, cyhalofop-butyl, diclofop-methyl, fenoxaprop-P-ethyl, fluazifop-P-butyl, haloxyfop-methyl, haloxyfop-R-methyl), saflufenacil, sethoxydim, siduron, simazine, symmetrine, sulcotrione, sulfentrazone, tebuthiuron, tembotrione, tepraloxydim, terbacil, terbumetone, terbuthylazine, taxtomine (for example, the taxtomines described in patent no. 2 US 7,989,393), tenylchlor, tralkoxydim, triclopyr, trietazine, tropramezone, their salts and esters; their racemic mixtures and resolved isomers and their combinations. In some embodiments, the inoculant compositions of the present disclosure comprise acetochlor, clethodim, dicamba, flumioxazin, fomesafen, glyphosate, glufosinate, mesotrione, quizalofop, saflufenacil, sulcotrione, S-3100 and / or 2,4-D. In some embodiments, the inoculant compositions of the present disclosure comprise glyphosate, glufosinate, dicamba, 2,4-D, acetochlor, metolachlor, pyroxasulfone, flumioxazin, fomesafen, lactofen, metribuzin, mesotrione and / or 2-((3-(2-chloro-4-fluoro-5-(3-methyl- Ethyl 2,6-dioxo-4-(trifluoromethyl)-2,3-dihydropyrimidin-1(6H)-yl)phenoxy)pyridin-2-yl)oxy)acetate. In some embodiments, the inoculant compositions of the present disclosure comprise one or more acetyl CoA carboxylase (ACCase) inhibitors, acetolactate synthase (ALS) inhibitors, acetohydroxy acid synthase (AHAS) inhibitors, photosystem II inhibitors, photosystem II inhibitors I, protoporphyrinogen oxidase (PPO or Protox) inhibitors, carotenoid biosynthesis inhibitors, enolpyruvyl shikimate-3-phosphate (EPSP) synthase inhibitor, glutamine synthetase inhibitor, dihydropteroate synthetase inhibitor, mitosis inhibitors, 4 -hydroxyphenyl-pyruvate-dioxygenase (4-HPPD), synthetic auxins, auxin herbicide salts, auxin transport inhibitors, nucleic acid inhibitors and / or one or more salts, esters, racemic mixtures and / or dissolved isomers thereof .
[00129] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chemical insecticides and / or nematicides. Non-limiting examples of chemical insecticides and nematicides include acrinathrin, alpha-cypermethrin, beta-cyfluthrin, cyhalothrin, cypermethrin, deltamethrin, csfenvalcrate, etofenprox, fenpropathrin, fenvalerate, flucitrinate, fostiazate, lambda-cyhalothrin, gamma-cyhalothrin, permethrin , tau-fluvalinate, transfluthrin, zeta-cypermethrin, ciflutri, bifenthrin, tefluthrin, eflusilanate, fubfenprox, pyrethrin, resmethrin, imidacloprid, acetamiprid, thiamethoxam, nitenpyram, thiacloprid, dinotefuran, clothianidin, imidaclotiz, chlorfluazuron, diflubenzuron, lufenuron, teflubenzomurom, triflumururon , novaluron, flufenoxurom, hexaflumuron, bistrifluorom, noviflumuron, buprofezin, cyromazine, methoxyfenozide, tebufenozide, halofenozide, chromafenozide, endosulfan, fipronil, ethiprol, pyrafluprole, pyriprol, flubendiamide, chlorantraniliprole (e.g. Rinaxipyr), cyazipyr, emamectin, emamectin benzoate, abamectin, ivermectin, milbemectin, lepimectin, tebufenpyrad, fenpyroximate, pyridaben, phenazaquin, pyrimidiphene, tolfenpyrad, dicofol, cyenopyraphene, cyflumethofen, acequinocil, fluacripyrine, bifenazate, diafenthiuron, ethoxazole, clofentezine, spinosad, tetradifomratene propargite, hexythiazox, bromopropylate, qinomethionate, amitraz, pyrifluquinazom, pymetrozine, flonicamid, pyriproxyfen, diofenolan, chlorfenapyr, metaflumizone, indoxacarb, chlorpyrifos, spirodiclofen, spiromesifen, spirotetramate, pyridalyl, spinctoram, acephate, triazophos, profenophos, oxamyl, phenatoramil, spinetoramil phenamipclothiahos, 4-{[(6-chloropyrid-3-yl)methyl](2,2-difluoroethyl)amino}furan-2(5H)-one, cadusaphos, carbaryl, carbofuran, ethoprophos, thiodicarb, aldicarb, aldoxycarb, methamidophos , methiocarb, sulfoxaflor, cyantraniliprole and thioxazophene and combinations thereof. In some embodiments, the inoculant compositions of the present disclosure comprise abamectin, aldicarb, aldoxycarb, bifenthrin, carbofuran, chlorantraniliprole, clothianidin, cyfluthrin, cyhalothrin, cypermethrin, cyantraniliprole, deltamethrin, dinotefuran, emamectin, ethiprol, fenamiphos, fipronil, flubendiamide, fostiazate , imidacloprid, ivermectin, lambda-cyhalothrin, milbemectin, nitenpyram, oxamil, permethrin, spinetoram, spinosad, spirodiclofen, spirotetramate, tefluthrin, thiacloprid, thiamethoxam and / or thiodicarb. In some embodiments, the inoculant compositions of the present disclosure comprise one or more carbamates, diamides, macrocyclic lactones, neonicotinoids, organophosphates, phenylpyrazoles, pyrethrins, spinosyns, synthetic pyrethroids, tetronic acids and / or tetramic acids. In some embodiments, the inoculant compositions of the present disclosure comprise an insecticide selected from the group consisting of clothianidin, thiamethoxam, imidacloprid, cyantraniliprole, chlorantraniliprole, fluopyram and thioxazaphene.
[00130] In some embodiments, the inoculant compositions of present disclosure comprises one or more biopesticides (e.g., one or more biofungicides, bioinsecticides and / or bionematicides). Examples of microbial strains that exhibit biopesticide activity are included in Appendix A, along with strains that exhibit nitrogen fixation activity, phosphate solubilization activity, etc. Additional examples of pesticides can be found in Bradley, Managing Diseases, in Illinois Agronomy Handbook (2008); Hager, Weed Management, in Illinois Agronomy Handbook (2008); Loux et al., Weed Control Guide for Ohio, Indiana and Illinois (2015); Niblack, Nematodes, in Illinois Agronomy Handbook (2008); and Steffey and Gray, Managing Insect Pests, emlliNOis Agronomy Handbook (2008).
[00131] Inoculum compositions of the present disclosure may comprise any suitable plant signal molecule (or molecules).
[00132] The inoculant compositions of the present disclosure may comprise any suitable LCO (or LCOs). LCOs, sometimes referred to as symbiotic nodulation (Nod) signals or Nod factors, consist of an oligosaccharide backbone of 0-1,4-linked A-acetyl-D-glucosamine residues ("GlcNAc") with a chain of N-linked fatty acyl condensed at the non-reducing end. LCOs differ in the number of GlcNAc residues in the backbone, the length and degree of saturation of the fatty acyl chain, and the substitutions of reducing and non-reducing sugar residues. See, for example, Denarie, et al., Ann. Rev. Biochem. 65: 503 (1996); Hamel, et al., Planta 232: 787 (2010); Prome, et al., Pure &Appl. chem. 70(1): 55 (1998).
[00133] In some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs represented by formula I: wherein G is a hexosamine which may be substituted, for example, by an acetyl group on nitrogen, a sulfate, an acetyl group and / or an ether group on an oxygen; R1, R2, R3, R5, R6 and R7, which may be identical or different, represent H, CH3 CO—, C x H y CO— where x is an integer from 0 to 17 and y is an integer from 1 to 35 or any other acyl group such as, for example, a carbamoyl; R4 represents a saturated or mono-, di- or tri-unsaturated aliphatic chain containing at least 12 carbon atoms; and n is an integer between 1 and 4.
[00134] In some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs represented by formula II: HC HC-,__ ^•(CH 2 🇧🇷 5 V (id in which R represents H or CH3CO— and n is equal to 2 or 3. See, for example, U.S. Patent No. 5,549,718. A number of LCOs derived from Bradyrhizobium japonicum have also been described, including BjNod-V (Ci8:i), BjNod-V (Ac, Ci8:i), BjNod-V (Ci 6: i) and BjNod-V (A c , ci 6:0 ) (with “V” indicating the presence of five N-acetylglucosamines, “Ac” an acetylation, the number after the “C” indicating the number of carbons in the fatty acid side chain, and the number after the indicating the number of double bonds ). See, for example, U.S. Patents Us. 5,175,149 and 5,321,011. Additional LCOs obtained from bacterial strains include NodRM, NodRM-1, NodRM-3. When acetylated (the R₁CHsCO—) become AcNodRM-1 and AcNodRM-3, respectively (U.S. Patent No. 5,545,718).
[00135] In some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs represented by formula III: O. O. in which n = 1 or 2; R1 represents C16, C16:0, C16:1, C16:2, C18:0, C18:1A9Z or C18:1A11Z; and R2 represents hydrogen or SO3H.
[00136] In some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs represented by formula IV: (IV) in which Ri represents C14:0, 3OH-C14:0, iso-C15:0, C16:0, 3-OH-C16:0, iso-C15:0, C16:1, C16:2, C16:3, iso-C17:0, iso-C17:1, C18:0, 3OH-C18:0, C18:0 / 3-OH, C18:1, OH-C18:1, C18:2, C18:3, C18: 4, C19:1 carbamophthalate, C20:0, C20:1, 3-OH-C20:1, C20:1 / 3-OH, C20:2, C20:3, C22:1, and C18-26(cu- 1)-OH (which, according to D'Haeze, et al., Glycobiology 12: 79R-105R (2002), includes hydroxylated species C18, C20, C22, C24 and C26 and C16:1A9, C16:2 (A2 ,9) and C16:3 (A2,4,9)); R2 represents hydrogen or methyl; R3 represents hydrogen, acetyl or carbamoyl; R4 represents hydrogen, acetyl or carbamoyl; R5 represents hydrogen, acetyl or carbamoyl; R6 represents hydrogen, arabinosyl, fucosyl, acetyl, SO3H, sulfate ester, 3-0-S-2-0-MeFuc, 2-0-MeFuc, and 4-0-AcFuc; R7 represents hydrogen, mannosyl or glycerol; Rs represents hydrogen, methyl, or -CH2OH; R9 represents hydrogen, arabinosyl, or fucosyl; Rio represents hydrogen, acetyl or fucosyl; and n represents 0, 1, 2, or 3. The naturally occurring LCOs encompassed by this structure are described in D'Haeze, et al., supra.
[00137] Additional examples of LCOs that may be useful in the compositions and methods of the present disclosure are provided below as (XVII) (XX) O. (XXIII) (XXIV) (XXV) (XXVI) (XXVII) (XXVIII) (XXIX) (XXX) (XXXI) (XXXIII).
[00138] LCOs may be obtained from any suitable source. In some embodiments, LCO is obtained (i.e., isolated and / or purified) from a bacterial strain. For example, in some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs obtained from an Azorhizobium, Bradyrhizobium (e.g., B. japonicum), Mesorhizobium, Rhizobium (e.g., R. leguminosarum) or Sinorhizobium (e.g., R. leguminosarum). example, S. meliloti). In some embodiments, the LCO is obtained (i.e., isolated and / or purified) from a mycorrhizal fungus. For example, in some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs obtained from a strain of Glomerocycota (e.g., Glomus intraradicus). See, for example, WO 2010 / 049751 (in which LCOs are referred to as "Myc factors"). In some embodiments, the LCO is synthetic. For example, in some embodiments, the inoculant compositions of the present disclosure comprise one or more of the synthetic LCOs described in WO 2005 / 063784, WO 2007 / 117500 and / or WO 2008 / 071674. In some embodiments, the synthetic LCO contains one or more modifications or substitutions, such as those described in Spaink, Crit. Rev. Plant Sci. 54: 257 (2000) and D'Haeze, supra. LCOs and precursors for building LCOs (eg COs, which are themselves useful as plant signaling molecules) can be synthesized by genetically modified organisms. See, for example, Samain et al., Carbohydrate Res. 302: 35 (1997); Cottaz, et al., Meth. Eng. 7(4):311 (2005); and Samain, et al., J. Biotechnol. 72: 33 (1999) (for example, Fig. 1 therein, which shows structures of COs that can be produced recombinantly in E. coli carrying different combinations of nodBCHL genes).
[00139] It is to be understood that the compositions and methods of the present disclosure may comprise analogues, derivatives, hydrates, isomers, salts and / or solvates of LCOs. Thus, in some modalities, the compositions inoculants of the present disclosure comprise one, two, three, four, five, six, seven, eight, nine, ten or more LCOs represented by one or more of formulas I-IV and / or structures V-XXXIII and / or one, two , three, four, five, six, seven, eight, nine, ten or more analogues, derivatives, hydrates, isomers, salts and / or solvates of LCOs represented by one or more of formulas I-IV and / or structures V-XXXIII .
[00140] LCOs (and their derivatives) can be used in various forms of purity and can be used alone or in the form of a culture of LCO-producing bacteria or fungi. In some embodiments, the LCO (or LCOs) included in the inoculant compositions of the present disclosure is at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5% or more pure.
[00141] Inoculating compositions of the present disclosure may comprise any suitable chitin oligomer (or oligomers) and / or chitosan oligomer (or oligomers). See, for example, D'Haeze et al., Glycobiol. 12(6):79R (2002); Demont-Caulet et al., Plant Physiol. 120(1):83 (1999); Hanel et al., Planta 232: 787 (2010); Muller et al., Plant Physiol. 124: 733 (2000); Robina et al., Tetrahedron 58: 521-530 (2002); Rouge et al., Docking of Chitin Oligomers and Nod Factors on Lectin Domains of the LysM-RLK Receptors in the Medicago-Rhizobium Symbiosis, in The Molecular Immunology of Complex Carbohydrates-3 (Springer Science, 2011); Van der Holst et al., Curr. opinion Struc. Biol. 11: 608 (2001); Wan et al., Plant Cell 21: 1053 (2009); and PCT / F100 / 00803 (2000).
[00142] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitin oligosaccharides represented by formula XXXIV: (XXXIV) in which Ri represents hydrogen or methyl; R2 represents hydrogen or methyl; R3 represents hydrogen, acetyl or carbamoyl; R4 represents hydrogen, acetyl or carbamoyl; R5 represents hydrogen, acetyl or carbamoyl; R6 represents hydrogen, arabinosyl, fucosyl, acetyl, sulfate ester, 3-0-S-2-0-MeFuc, 2-0-MeFuc, and 4-0-AcFuc; R7 represents hydrogen, mannosyl or glycerol; Rs represents hydrogen, methyl, or -CH2OH; R9 represents hydrogen, arabinosyl, or fucosyl; Rio represents hydrogen, acetyl or fucosyl; and n represents 0, 1, 2 or 3.
[00143] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitin oligosaccharides (XXXV) in which n = 1 or 2; Ri represents hydrogen or methyl; and R2 represents hydrogen or SO3H.
[00144] Additional examples of oligosaccharides (and derivatives thereof) that may be useful in the compositions and methods of the present disclosure are given below as structures XXXVI to LXXXIII: (XXXVI) 0' 'CHx (XXXVII) (XXXVIII) (XXXIX) 0200007305, (XXXXIV) (XXXXV) (XXXXVI) (XXXXVII) (LUI) (LIV) oh so 3 'At + (LVIII) (LIX) (LX) (LXI) (LXII) (LXIII) H (LXV) (LXVII) (LXVIII) (LXIX) (LXXI) (LXXII) (LXXIII) (LXXIV) (LXXV) (LXXVI) (LXXVII) (LXXVIII) (LXXIX) (LXXX) (LXXXI) (LXXXII) (LXXXIII)
[00145] In some embodiments, the inoculant compositions of the present disclosure comprise one or more of the oligosaccharides set forth above as structures XXXVI to LXXXIII in a deacetylated form (e.g., an oligosaccharide that corresponds to structure XXXVI above, except that one or more of the groups acetyl have been removed, optionally replaced with a hydrogen or methyl group).
[00146] Chitin oligosaccharides and chitosan oligosaccharides can be obtained from any suitable source. Chitin oligosaccharides and chitosan oligosaccharides can be harvested from chitin / chitosan (see, for example, Aam et al., Mar. DRugs 8:1482 (2010); D'Haeze et al., Glycobiol. 12(6 ):79R (2002); Demont-Caulet et al., Plant Physiol.120(1):83 (1999); Hanel et al., Planta 232:787 (2010); Limpanavech et al., Scientia Horticultural 116:65 (2008); Lodhi et al., BioMed Res. Intl. Volume 2014, Article 654913 (March 2014); Mourya et al., Polymer Sci. 53(7):583 (2011); Muller et al., Plant Physiol 124:733 (2000); Robina et al., Tetrahedron 58:521 (2002); Rouge et al., The Molecular Immunology of Complex Carbohydrates, in Advances in Experimental Medicine and Biology (Springer Science, 2011); Van der Holst et al., Curr. Opin. Struc. Biol. 11:608 (2001); Wan et al., Plant Cell 21:1053 (2009); Xia et al., Food Hydrocolloids 25:170 (2011); PCT / F100 / 00803 (2000)). They can also be generated synthetically (see, for example, Cottaz et al., Meth. Eng. 7(4):311 (2005); Samain et al., Carbohydrate Res. 302:35 (1997.); Samain et al., J. Biotechnol.72:33 (1999). In some embodiments, they are derived from a naturally occurring LCO. For example, in some embodiments, the inoculant compositions of the present disclosure comprise one or more oligosaccharides of chitin / chitosan derived from an LCO obtained (i.e. isolated and / or purified) from a strain of Azorhizobium, Bradyrhizobium (e.g. B. japonicum), Mesorhizobium, Rhizobium (e.g. eg R. leguminosarum), Sinorhizobium (e.g. S. meliloti) or mycorrhizal fungus (e.g. Glomus intraradicus). In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitin oligosaccharides and / or chitosan oligosaccharides derived from an LCO obtained (i.e., isolated and / or purified) from a strain of Azorhizobium, Bradyrhizobium (e.g., B japonicum), Mesorhizobium, Rhizobium (eg R. leguminosarum), Sinorhizobium (eg S. meliloti), or mycorrhizal fungus (eg Glomus intraradicus). In some embodiments, the chitosan oligosaccharide (or oligosaccharides) and / or the chitosan oligosaccharide (or oligosaccharides) are derived from an LCO represented by one or more of formulas I to IV and / or structures V to XXXIII. Thus, in some embodiments, inoculant compositions of the present disclosure may comprise one or more chitin oligosaccharides represented by one or more of formulas I to IV and / or structures V to XXXIII except that the pendant fatty acid is replaced by a hydrogen or methyl group.
[00147] It should be understood that the compositions of the present disclosure may comprise analogues, derivatives, hydrates, isomers, salts and / or solvates of chitin oligosaccharides and / or chitosan oligosaccharides. Thus, in some embodiments, inoculant compositions of the present disclosure comprise one, two, three, four, five, six, seven, eight, nine, ten or more chitin oligosaccharides represented by one or more of formulas XXXIV to XXXV and / or or structures XXXVI to LXXXIII and / or one, two, three, four, five, six, seven, eight, nine, ten or more analogues, derivatives, hydrates, isomers, salts and / or solvates of chitin oligosaccharides represented by one or more of formulas XXXIV to XXXV and / or structures XXXVI to LXXXIII.
[00148] Chitin oligosaccharides and chitosan oligosaccharides (and analogues, derivatives, hydrates, isomers, salts and / or solvates of themselves) can be used in various forms of purity and can be used alone or in the form of a culture of CO-producing bacteria or fungi. In some embodiments, the chitin oligosaccharides and / or chitosan oligosaccharides included in the inoculant compositions of the present disclosure are at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90 %, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5% or more pure.
[00149] The inoculant compositions of the present disclosure may comprise any suitable chitinous compound (or compounds), including, without limitation, chitin (IUPAC: N-[5-[[3-acetylamino-4,5-dihydroxy-6- (hydroxymethyl)oxan-2-yl]methoxymethyl]-2-[[5-acetylamino-4,6-dihydroxy-2-(hydroxymethyl)oxan-3-yl]methoxymethyl]-4-hydroxy-6-(hydroxymethyl) )oxan-3-yl]ethanamide), chitosan (IUPAC: 5-amino-6-[5-amino-6-[5-amino-4,6-dihydroxy-2-(hydroxymethyl)oxan-3-yl ]oxy-4-hydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-2-(hydroxymethyl)oxane-3,4-diol) and its isomers, salts and solvates.
[00150] Chitins and chitosans can be obtained commercially or prepared from insects, crustacean shells or fungal cell walls. Methods for preparing chitin and chitosan are known in the art. See, for example, U.S. Patents Us. 4,536,207 (preparation from crustacean shells) and 5,965,545 (preparation from crustacean shells and hydrolysis of commercial chitosan); Pochanavanich, et al., Lett. app. Microbiol. 35: 17 (2002) (preparation from fungal cell walls).
[00151] Compositions of chitin and chitosan formulated for seed treatment are commercially available. Commercial products include, for example, ELEXA® (Plant Defense Boosters, Inc.) and BEYOND™ (Agrihouse, Inc.).
[00152] The inoculant compositions of the present disclosure can means any suitable flavonoid (or suitable flavonoids), including, but not limited to, anthocyanidins, anthoxanthins, chalcones, coumarins, flavanones, flavanonols, flavanes and isoflavonoids, as well as analogues, derivatives, hydrates, isomers, polymers, salts and solvents thereof.
[00153] Flavonoids are phenolic compounds having the general structure of two aromatic rings connected by a three-carbon bridge. Classes of flavonoids include are known in the art. See, for example, Jain et al., J. Plant Biochem. & Biotechnol. 11:1 (2002); Shaw et al., Environ. Microbiol. 11: 1867 (2006). Flavonoid compounds are commercially available, for example, from Novozymes BioAg, Saskatoon, Canada; Natland International Corp., Research Triangle Park, NC; MP Biomedicals, Irvine, CA; LC Laboratories, Wobum MA. Flavonoid compounds can be isolated from plants or seeds, for example, as described in U.S. Pat. 5,702,752; 5,990,291; and 6,146,668. Flavonoid compounds can also be produced by genetically engineered organisms, such as yeast, as described in Ralston, et al., Plant Physiology 137: 1375 (2005).
[00154] In some embodiments, the inoculant compositions of the present disclosure comprise one or more anthocyanidins. According to some embodiments, the inoculant composition comprises cyanidin, delphinidin, malvidin, pelargonidin, peonidin and / or petunidin.
[00155] In some embodiments, the inoculant compositions of the present disclosure comprise one or more anthoxanthins. According to some embodiments, the inoculant composition comprises one or more flavones (e.g., apigenin, baicalein, chrysin, 7,8-dihydroflavone, diosmin, flavoxate, 6-hydroxyflavone, luteolin, scutelarein, tangeritin and / or wogonin) and / or flavonols (e.g., amurensin, astragalin, azaleatin, azalein, fisetin, furanoflavonols galangin, gossipetin, 3- hydroxyflavone, hyperoside, icariin, isoquercetin, kaempferide, kaempferitrin, kaempferol, isorhamnetin, morphine, myricetin, myricitrin, natsudaidaine, paquipodol, pyranoflavonols quercetin, quercetin, ramnazine, ramnetin, robinin, rutin, spiraeoside, troxerutin and / or zanthoramnin).
[00156] In some embodiments, the inoculant compositions of the present disclosure comprise one or more flavanones. According to some embodiments, the inoculant composition comprises butin, eriodictyol, hesperetin, hesperidin, homoeryodictyol, isossacuranetin, naringenin, naringin, pinocembrine, poncyrin, sacuranetin, sacuranetin and / or sterubin.
[00157] In some embodiments, the inoculant compositions of the present disclosure comprise one or more flavanonols. According to some embodiments, the inoculant composition comprises dihydrokaempferol and / or taxifolin.
[00158] In some embodiments, the inoculant compositions of the present disclosure comprise one or more flavanes. According to some embodiments, the inoculant composition comprises one or more flavan-3-ols (e.g., catechin (C), catechin 3-gallate (Cg), epicatechins (EC), epigallocatechin (EGC) epicatechin 3-gallate (ECg), epigalcatechin 3-gallate (EGCg), epiafzelechin, fisetinidol, gallocatechin (GC), galcatechin 3-gallate (GCg), guibourtinidol, mesquitol, robinetinidol, teaflavin-3-gallate, teaflavin-3'-galate, teflavin-3,3'-digalate, thearubigin), flavan-4-ols (e.g. apiphorol and / or luteophorol) and / or flavan-3,4-diols (e.g. leukocyanidin, leukodelphinidin, leukophysetinidin, leucomalvidin, leucopelargonidin , leucopeonidin, leucorobinetinidin, melacacidin and / or teracacidin) and / or dimers, trimers, oligomers and / or polymers thereof (e.g. one or more proanthocyanidins).
[00159] In some embodiments, the inoculant compositions of the present disclosure comprise one or more isoflavonoids. According to some embodiments, the inoculant composition comprises one or more isoflavones (eg biocanin A, daidzein, formononetin, genistein and / or glycitein), isoflavans (eg equol, ioncocarpan and / or laxiflo-orane), isoflavandiols, isoflavenes (eg glabrene, haginine D and / or 2 -methoxyjudaicin), coumestans (e.g. coumestrol, plicadine and / or wedelolactone), pterocarpans and / or rotenoids.
[00160] The inoculant compositions of the present disclosure may comprise any suitable flavonoid derivative, including, but not limited to, neoflavonoids (e.g., calophyllolide, coutareagenin, dalbergicromene, dalbergin, nivetin) and pterocarpanes (e.g., bitucarpine A, bitucarpine B, eribraedin A, eribraedin B, erythrabissin II, erythrabissin-1, erycristagalin, glycinol, glyceolidines, glyceolins, glycyrrhizole, maachiaine, medicarpine, morisianin, orientalol, phaseolin, pisatin, striatin, trifolyrizine).
[00161] Flavonoids and derivatives thereof may be incorporated into the inoculant compositions of the present disclosure in any suitable form, which includes, without limitation, polymorphic and crystalline forms.
[00162] The inoculant compositions of the present disclosure may comprise any suitable non-flavonoid nod gene inducer (or suitable non-flavonoid nod gene inducers), including, but not limited to, jasmonic acid ([lR-[la,2P(Z) ]]-3-oxo-2-(pentenyl)cyclopentanoacetic; JA), linoleic acid ((Z,Z)-9,12-octadecadienoic acid) and linolenic acid ((Z,Z,Z)-9,12 acid, 15-octadecatrienoic), as well as analogues, derivatives, hydrates, isomers, polymers, salts and solvates thereof.
[00163] Jasmonic acid and its methyl ester, methyl jasmonate (MeJA), collectively known as jasmonates, are octadecanoid-based compounds that occur naturally in some plants (e.g. wheat), fungi (e.g. Botryodiplodia theobromae, Gibbrella fujikuroi), yeast (e.g. Saccharomyces cerevisiaé) and bacteria (e.g. Escherichia coli). Linoleic acid and linolenic acid are produced in the course of jasmonic acid biosynthesis. Jasmonates, linoleic acid and linolenic acid (and their derivatives) are reported to be inducers of nod gene expression or LCO production by rhizobacteria. See, for example, Mabood, et al. Plant Physiol. Biochem. 44(11):759(2006); Mabood et al., Agr. J. 98(2): 289 (2006); Mabood, et al., Field Crops Res. 95 (2 and 3): 412 (2006); Mabood & Smith, Linoleic and linolenic acid induce the expression of nod genes in Bradyrhizobium japonicum USDA 3, Plant Biol. (2001). Non-limiting examples of jasmonic acid, linoleic acid, linolenic acid derivatives include esters, amides, glycosides and salts. Representative esters are compounds in which the carboxyl group of linoleic acid, linolenic acid, or jasmonic acid has been replaced by a —COR group, where R is a --OR group 1 , in which R 1 is: an alkyl group, such as an unbranched or branched C1 -C8 alkyl group, for example a methyl, ethyl or propyl group; an alkenyl group, such as an unbranched or branched Cz-Cs alkenyl group; an alkynyl group, such as an unbranched or branched Cz-Cs alkynyl group; an aryl group having, for example, 6 to 10 carbon atoms; or a heteroaryl group having, for example, 4 to 9 carbon atoms, where the heteroatoms in the heteroaryl group can be, for example, N, O, P or S. Representative amides are compounds in which the carboxyl group of linoleic acid, linolenic acid or jasmonic acid has been replaced by a —COR group, where R is an NR group 2 R 3 , in which R 2 and R 3 are independently: hydrogen; an alkyl group, such as an unbranched or branched C1 -C8 alkyl group, for example a methyl, ethyl or propyl group; an alkenyl group, such as an unbranched or branched Cz-Cs alkenyl group; an alkynyl group, such as an unbranched or branched C2-Cs alkynyl group; an aryl group having, for example, 6 to 10 carbon atoms; or a heteroaryl group having, for example, 4 to 9 carbon atoms, wherein the heteroatoms in the heteroaryl group may be, for example for example, N, O, P or S. Esters can be prepared by known methods, such as acid-catalyzed nucleophilic substitution, in which the carboxylic acid is reacted with an alcohol in the presence of a catalytic amount of a mineral acid. Amides can also be prepared by known methods, such as by reacting the carboxylic acid with the appropriate amine in the presence of a coupling agent such as dicyclohexyl carbodiimide (DCC), under neutral conditions. Suitable salts of linoleic acid, linolenic acid and jasmonic acid include, for example, base addition salts. Bases that can be used as reagents to prepare metabolically acceptable base salts of these compounds include those derived from cations such as alkali metal cations (eg, potassium and sodium) and alkaline earth metal cations (eg, calcium and magnesium). These salts can be readily prepared by mixing together a solution of linoleic acid, linolenic acid or jasmonic acid with a solution of the base. Salts can be precipitated from solution and collected by filtration or they can be recovered by other means such as solvent evaporation.
[00164] The inoculant compositions of the present disclosure may comprise any suitable carraquin (or carraquinas), including, but not limited to, 2H-furo[2,3-c]pyran-2-ones, as well as analogues, derivatives, hydrates, isomers , polymers, salts and solvates thereof.
[00165] In some embodiments, the inoculant composition comprises one or more carrachines represented by formula LXXXIV: r 3 ^r 4 (LXXXIV) in which Z is O, S or NR5; Ri, R2, R3 and R4 are each independently H, alkyl, alkenyl, alkynyl, phenyl, benzyl, hydroxy, hydroxyalkyl, alkoxy, phenyloxy, benzyloxy, CN, CORô, COOR=, halogen, NRóR? or NO2; and R5 , R6 and R7 are each independently H, alkyl or alkenyl or a biologically acceptable salt thereof.
[00166] Examples of biologically acceptable salts of carrikins include acid addition salts formed with biologically acceptable acids, examples of which include hydrochloride, hydrobromide, sulfate or bisulfate, phosphate or hydrogen phosphate, acetate, benzoate, succinate, fumarate, maleate, lactate, citrate, tartrate, gluconate; methanesulfonate, benzenesulfonate and p-toluenesulfonic acid. Additional biologically acceptable metal salts can include alkali metal salts with bases, examples of which include the sodium and potassium salts. Examples of compounds encompassed by formula XXXX that may be suitable for use in the present disclosure include 3-methyl-2H-furo[2,3-c]pyran-2-one (where Ri=CH;. R 2 , R3, R 4 =H). 2H-furo[2,3-c]pyran-2-one (where Ri, R 2 , R3, R4=H), 7-methyl-2H-furo[2,3-c]pyran-2-one (where Ri, R 2 , R 4 =H,R 3 =CH3), 5-methyl-2H-furo[2,3-c]pyran-2-one (where Ri, R 2 , Rs=H, R^CHs), 3,7-dimethyl-2H-furo[2,3-c]pyran-2-one (where Ri, R3=CH 3 , R 2 , R 4 =H), 3,5-dimethyl-2H-furo[2,3-c]pyran-2-one (where Ri, R 4 =CHi,R 2 , R 3 =H), 3,5,7-trimethyl-2H-furo[2,3-c]pyran-2-one (where R1, R3, R 4 =CHi,R 2 =H), 5-methoxymethyl-3-methyl-2H-furo[2,3-c]pyran-2-one (where Ri=CH;. R 2 , R;=H. I-^CIUOCH;). 4-bromo-3,7-dimethyl-2H-furo[2,3-c]pyran-2-one (where Ri, Ri=CH3, R 2 =Br, R 4 =H), 3-methylfuro[2,3-c]pyridin-2(3H)-one (where Z=NH, R,=CH 3 , R 2 , R 3 , R 4 =H) and 3,6-dimethylfuro[2,3-c]pyridin-2(6H)-one (where Z=N—CH3, Ri=CHi, R 2 , R 3 , R 4 =H). See, for example, U.S. Patent No. 7,576,213; Halford, Smoke Signals, in Chem. Eng. News (April 12, 2010) (reporting that the carrikins or butenolides contained in the smoke act as growth stimulants and stimulate seed germination after a wildfire and can invigorate seeds such as corn, tomatoes, lettuce and onions that had been stored).
[00167] The inoculant compositions of the present disclosure may comprise gluconolactone and / or one or more analogues, derivatives, hydrates, isomers, polymers, salts and / or solvates thereof.
[00168] The inoculant compositions of the present disclosure may comprise any suitable excipient (or excipients), including, but not limited to, dispersants, drying agents, antifreeze agents, seed flow agents, protection agents, antisettling agents, pH buffers and stickers.
[00169] The inoculant compositions of the present disclosure may comprise any suitable agriculturally acceptable dispersant (or dispersants), including, but not limited to, surfactants and wetting agents. Selection of appropriate dispersants will depend on the intended application (or applications) and the microorganism (or microorganisms) present in the inoculant composition. Generally, the dispersant (dispersants) will have low toxicity to the microorganism (microorganisms) in the inoculant composition and to the plant part (or parts) to which the inoculant composition is to be applied. In some embodiments, the dispersant (or dispersants) will be selected to wet and / or emulsify one or more soils. Non-limiting examples of dispersants include ATLOX™ (e.g., 4916, 4991; Croda International PLC, Edison, NJ), ATLOX METASPERSE™ (Croda International PLC, Edison, NJ), BIO-SOFT® (e.g., N series, as Nl-3, Nl-7, Nl-5, Nl-9, N23-3, N2,3-6,5, N25-3, N25-7, N25-9, N91-2,5, N91-6, N91-8; Stepan Company, Northfield, IL), MAKON® nonionic surfactants (eg, DA-4, DA-6, and DA-9; Stepan Company, Northfield, IL), WET® MOR powders (Akzo Nobel Surface Chemistry LLC, Chicago, IL), MULTIWET™ Surfactants (eg, MO-85P-PW-(AP); Croda International PLC, Edison, NJ), SILWET® L-77 (Helena Chemical Company, Collierville, TN), SPAN Surfactants ™ (e.g., 20, 40, 60, 65, 80, and 85; Croda Inc., Edison NJ), TAMOL™ dispersants (The Dow Chemical Company, Midland, MI), TERGITOL™ surfactants (eg, TMN-6 and TMN-100X; The Dow Chemical Company, Midland, MI), TERSPERSE surfactants (eg, 2001, 2020, 2100, 2105, 2158, 2700, 4894 and 4896; Hunstman Corp., The Woodlands, TX), TRITON™ Surfactants (e.g., X-100; The Dow Chemical Company, Midland, MI), TWEEN® Surfactants (for TWEEN® 20, 21, 22, 23, 28, 40, 60, 61, 65, 80, 81 and 85; Croda International PLC, Edison, NJ) and combinations thereof. Additional examples of dispersants can be found in Baird & Zublena. 1993. Soil Facts: Using Wetting Agents (Nonionic Surfactants) on Soil (North Carolina Cooperative Extension Service Publication AG-439-25) (1993); Burges, Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments (Springer Science & Business Media) (2012); McCarty, Wetting Agents (Clemson University Cooperative Extension Service Publication) (2001).
[00170] In some embodiments, the inoculant compositions of the present disclosure comprise one or more anionic surfactants. According to some embodiments, the inoculant composition comprises one or more water-soluble anionic surfactants and / or one or more water-insoluble anionic surfactants, optionally one or more anionic surfactants selected from the group consisting of alkyl carboxylates (e.g. , sodium stearate), alkyl sulfates (e.g., alkyl lauryl sulfate, sodium lauryl sulfate), alkyl ether sulfates, alkyl starch ether sulfates, alkyl aryl polyether sulfates, alkyl aryl sulfates, alkyl aryl sulfonates , alkyl sulfonates, alkyl amide sulfonates, alkyl aryl sulfonates, alkyl benzene sulfonates, alkyl diphenyloxide sulfonate, alpha-olefin sulfonates, alkyl naphthalene sulfonates, paraffin sulfonates, alkyl sulfosuccinates, alkyl ether sulfosuccinates, sulfosuccinates alkylamide, alkyl sulfosuccinamates, sulfoacetates alkyl phosphates, alkyl ether phosphates, acyl sarconinates, acyl isethionates, N-acyl taurates, N-acyl-N-alkyltaurates, benzene sulfonates, cumene sulfonates, dioctyl sodium sulfosuccinate, ethoxylated sulfosuccinates , lignin sulfonates, linear alkylbenzene sulfonates, monoglyceride sulfates, perfluorobutanesulfonate, perfluorooctanesulfonate, phosphate ester, styrene acrylic polymers, toluene sulfonates and xylene sulfonates.
[00171] In some embodiments, the inoculant compositions of the present disclosure comprise one or more cationic surfactants. According to some embodiments, the inoculant composition comprises one or more pH-dependent amines and / or one or more quaternary ammonium cations, optionally, one or more cationic surfactants selected from the group consisting of alkyltrimethylammonium salts (e.g., bromide cetyl trimethylammonium chloride, cetyl trimethylammonium chloride), cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, 5-bromo-5-nitro-1,3-dioxane, dimethyldioctadecylammonium chloride, cetrimonium bromide, dioctadecyldimethylammonium bromide and / or octenidine dihydrochloride.
[00172] In some embodiments, the inoculant compositions of the present disclosure comprise one or more nonionic surfactants. According to some embodiments, the inoculant composition comprises one or more water-soluble nonionic surfactants and / or one or more water-insoluble nonionic surfactants, optionally one or more nonionic surfactants selected from the group consisting of alcohol ethoxylates (for example, TERGITOL™ 15-S surfactants, such as TERGITOL™ 15-S-9 (The Dow Chemical Company, Midland, MI)), alkanolamides, alkanolamine condensates, carboxylic acid esters, cetostearyl alcohol, cetyl alcohol, cocamide DEA, dodecyldimethylamine oxides, ethanolamides, glycerol ester ethoxylates and glycol esters, ethylene oxide polymers, ethylene oxide-propylene oxide copolymers, ethylene oxide alkyl ethers glycoside, glycerol alkyl ethers, glycerol esters, glycol alkyl ethers (for example, polyoxyethylene glycol alkyl ethers, polyoxypropylene glycol alkyl ethers), glycol alkylphenol ethers (for example, polyoxyethylene glycol alkylphenol ethers), glycol esters, monolaurin, monododecyl ethers of pentaethylene glycol, poloxamer, polyamines, polyglycerol polyricinoleate, polysorbate, polyoxyethylene fatty acids, polyoxyethylene glycol mercaptans, polyoxyethylene glycols polyoxyethylene glycols, polyoxyethylene sorbitan alkyl esters, polyethylene glycol-polypropylene glycol copolymers, octylphenol ethers of polyoxyethylene glycol pyrolides, polyvinyl alkyl pyrolides, polyvinyl pyrolides sugar, sulfoanilamides, sorbitan fatty acid alcohol ethoxylates, sorbitan fatty acid ester ethoxylates, sorbitan fatty acid ester and / or tertiary acetylenic glycols.
[00173] In some embodiments, the inoculant compositions of the present disclosure comprise at least one nonionic surfactant. According to some embodiments, the inoculant composition comprises at least one water-insoluble nonionic surfactant and at least one water-soluble nonionic surfactant. In some embodiments, the inoculant compositions of the present disclosure comprise a combination of nonionic surfactants that have hydrocarbon chains of substantially the same length.
[00174] In some embodiments, the inoculant compositions of the present disclosure comprise one or more zwitterionic surfactants. According to some embodiments, the inoculant composition of the present disclosure comprises one or more betaines and / or one or more sultaines, optionally, one or more zwitterionic surfactants selected from the group consisting of 3-[(3-cholamidopropyl)dimethylammonium]- l-propanesulfonate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and / or one or more sphingomyelins.
[00175] In some embodiments, the inoculant compositions of the present disclosure comprise one or more soaps and / or organosilicone surfactants. According to some embodiments, the inoculant composition comprises one or more alkali metal salts of fatty acids.
[00176] In some embodiments, the inoculant compositions of the present disclosure comprise one or more wetting agents. According to some embodiments, the inoculant composition comprises one or more naphthalene sulfonates, optionally, one or more alkyl naphthalene sulfonates (e.g. sodium alkyl naphthalene sulfonate), one or more isopropyl naphthalene sulfonates (e.g. sodium sodium isopropyl naphthalene sulfonate) and / or one or more naphthalene butyl sulfonates (e.g. sodium naphthalene n-butyl sulfonate).
[00177] The inoculant compositions of the present disclosure may comprise any suitable drying agent (or agents), including, but not limited to, drying powders. Non-limiting examples of drying agents include AEROSIL® hydrophobic fumed silica powders (Evonik Corporation, Parsippany, NJ), BENTOLITE® powders (BYK-Chemie GmbH, Wesel, Germany), INCOTEC® powders (INCOTEC Inc., Salinas, CA), SIPERNAT® silica powders (Evonik Corporation, Parsippany, NJ) and combinations thereof. Additional examples of drying agents can be found in Burges, Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments (Springer Science & Business Media) (2012). In some embodiments, the inoculant compositions of the present disclosure comprise calcium stearate, clay (e.g., attapulgite clay, montmorillonite clay), graphite, magnesium stearate, magnesium sulfate, milk powder, silica (e.g., pyrolyzed silica , hydrophobically coated silica, precipitated silica), soy lecithin and / or talc.
[00178] The inoculant compositions of the present disclosure can comprise any suitable antifreeze agent (or agents), including, but not limited to, ethylene glycol, glycerine, propylene glycol and urea.
[00179] The inoculant compositions of the present disclosure can comprise any seed fluidizing agents to improve the lubricity of the treated seeds. The flow agent may comprise one or more liquid lubricants, solid lubricants, liquid emulsions or suspensions of solid lubricants. Non-limiting examples of flow agents include, for example, lubricants, such as fats and oils, natural and synthetic waxes, graphite, talc, fluoropolymers (for example, polytetrafluoroethylene), and solid lubricants, such as molybdenum disulfide and tungsten disulfide . In some cases, the flow agent comprises a waxy material. Non-limiting examples of wax materials that can be incorporated into the liquid seed treatment composition include waxes derived from plants and animals, such as carnauba wax, candelilla wax, ouricuri wax, beeswax, spermaceti and petroleum waxes. , such as paraffin wax. For example, in some cases, the flow agent comprises carnauba wax. In some cases, the flow agent comprises an oil. For example, the flow agent may comprise soybean oil and / or tung oil. Non-limiting examples of commercially available materials suitable for use as flow agents include AQUAKLEAN 418 available from Micro Powders, Inc. (an anionic aqueous emulsion comprising extra light carnauba wax at 35% solids content).
[00180] The inoculant compositions of the present disclosure may comprise any suitable safener (or safeners), including, but not limited to, naphthalic anhydride.
[00181] The inoculant compositions of the present disclosure may comprise any buffer(s) of suitable pH, including, but not limited to, potassium phosphate monobasic and potassium phosphate dibasic. In In some embodiments, the inoculant composition comprises one or more pH buffers selected to provide a composition that has a pH of less than 10, typically from about 4.5 to about 9.5, from about 6 to about 8, or about 7.
[00182] The inoculant compositions of the present disclosure may comprise any suitable antifouling agent (or suitable antifouling agents), including, but not limited to, polyvinyl acetate, polyvinyl alcohols with different degrees of hydrolysis, polyvinylpyrrolidones, polyacrylates, waterborne paint system binders based on acrylate, polyol or polyester which are soluble or dispersible in water, in addition, copolymers of two or more monomers, such as acrylic acid, methacrylic acid, itaconic acid, maleic acid, fumaric acid, maleic anhydride, vinylpyrrolidone, ethylenically unsaturated monomers , such as ethylene, butadiene, isoprene, chloroprene, styrene, divinylbenzene, o-methylstyrene or p-methylstyrene, additionally, vinyl halides, such as vinyl chloride and vinylidene chloride, additionally, vinyl esters, such as vinyl acetate, vinyl propionate or vinyl stearate, in addition vinyl methyl ketone or acid esters acrylic or methacrylic acid with monohydric alcohols or polyols, such as methyl acrylate, methyl methacrylate, ethyl acrylate, ethylene methacrylate, lauryl acrylate, lauryl methacrylate, decyl acrylate, N,N-dimethylaminoethyl methacrylate , 2-hydroxyethyl methacrylate, 2-hydroxypropyl methacrylate or glycidyl methacrylate, in addition, diethyl esters or monoesters of unsaturated dicarboxylic acids, in addition, (meth)acrylamido-N-methylol methyl ether, amides or nitriles, such as acrylamide, methacrylamide, N-methylol(meth)acrylamide, acrylonitrile, methacrylonitrile and also N-substituted maleimides and esters such as vinyl butyl ether, vinyl isobutyl ether or vinyl phenyl ether and combinations thereof.
[00183] The inoculant compositions of the present disclosure can comprise any suitable adhesive (or suitable adhesives), including, but not limited to, adhesive compositions comprising, consisting essentially of or consisting of one or more disaccharides (e.g. maltose), gums (e.g. cellulose gum, guar gum, arabic gum, combrite gum, xanthan gum), maltodextrins (e.g., one or more maltodextrins (each and / or collectively) having a DEV of about 10 to about 20), monosaccharides, oils (e.g., mineral oil, olive oil, peanut oil, soybean oil, sunflower oil and / or tung oil) and / or oligosaccharides.
[00184] The inoculant compositions of the present disclosure may comprise any suitable effect pigment (or pigments). Effect pigments, which are sometimes also referred to in the art as "pearling pigments", are a class of materials that provide reflectivity, luster and / or a pearlizing effect when applied as a coating. In some cases, the effect pigment is in the form of a powder comprising a substrate material and a metal oxide coating. For example, the effect pigment can comprise a substrate material including, but not limited to, talc, silicate materials (eg, mica), clay minerals, calcium carbonate, kaolin, phlogopite, alumina, and the like. In some cases, the substrate material comprises a hydrophilic material. The substrate material may be coated with a semi-transparent layer of metal oxide including, but not limited to, titanium dioxide, iron oxide, chromium oxide or zirconium oxide. Alternatively, in some cases, the effect pigment comprises metal powders and metal flakes. The metal powder or metal flakes can comprise a metal including, but not limited to, aluminum, copper, silver or bronze. In some cases, the effect pigment comprises a silicate-based substrate. Non-limiting examples of particulate silicates that can be incorporated into dry powder coating include dioxide coated mica titanium (for example, SUMMICA FINE WHITE 2800102, which is commercially available from Sun Chemical Corp.). Other non-limiting examples of commercially available effect pigments that can be incorporated into the dry powder include MAGNA PEARL, LUMINA and MEARLIN pigments from BASF Corporation; PHIBRO PEARL from PhibroChem; and IRIDESIUM 120 from Aakash Chemicals. In some cases, the dry powder has an average particle size of from about 1 to about 25 microns.
[00185] The inoculant compositions of the present disclosure may comprise any suitable growth medium that is suitable for culturing one or more of the microorganisms in the inoculant composition. For example, in some embodiments, the inoculant compositions of the present disclosure comprise Czapek-Dox medium, glycerol yeast extract, mannitol yeast extract, potato dextrose broth and / or YEM medium.
[00186] Stabilizing compounds, biostimulants, microbial extracts, nutrients, pest attractants and / or feeding stimulants, pesticides, LCOs, chitin oligomers, chitosan oligomers, chitins, chitosans, flavonoids, dispersants, drying agents, drying agents protection, flow agents, anti-settling agents, buffers, adhesives, etc. they can be incorporated into the inoculant compositions of the present disclosure in any suitable amount (or amounts) / concentration (or concentrations). The absolute value of the amount / concentration that is sufficient to cause the desired effect (or effects) can be affected by factors such as the type, size and volume of material to which the composition will be applied, the type (or types) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition, and storage conditions (eg, temperature, relative humidity, duration). Those skilled in the art will understand how to select effective amounts / concentrations using routine dose-response experiments. guidance for selection of appropriate amounts / concentrations can be found, for example, in International Patent Applications Nos. PCT / US2016 / 050529 and PCT / US2016 / 050647 and US Provisional Patent Applications 62 / 296,798; 62 / 271,857; 62 / 347,773; 62 / 343,217; 62 / 296,784; 62 / 271,873; 62 / 347,785; 62 / 347,794; and 62 / 347,805.
[00187] In some embodiments, the inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount / concentration of from about 0.0001 to about 95% or more (by weight, based on the total inoculant composition). For example, the inoculant compositions of the present disclosure can comprise about 0.0001 to about 0.001, about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 30 to about 60%, about 50 to about 75% or about 75 to about 95% (by weight), optionally about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05. 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of one or more maltodextrins, monosaccharides, disaccharides, sugar alcohols, humic acids, betaines, prolines, sarcosines, peptones , oxidation control components, hygroscopic polymers and / or UV protectants.
[00188] In some embodiments, the inoculant compositions of the present disclosure comprise one or more stabilizing compounds at a concentration of about 1 x 10' 20 M at about 1 x 10' 1 M. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10 15 M at about 1 x 10’ 10 M, about 1 x IO 14 M at about 1 x 10’ 8 M, about 1 x IO 14 M to about 1 x IO' 6 M, about 1 x IO 12 M to about 1 x l0' 8 M, about 1 x IO 12 M to about 1 x IO' 6 M, about 1 x 10' 10 M at about 1 x 10’ 6 M or about I x l0' 8 M to about 1 x IO' 2 M, optionally, about 1 x IO 20 M, 1 x IO 19 M, 1 x IO 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10' 15 M, 1 x IO 14 M, 1 x IO 13 M, 1 x IO 12 M, 1 x IO 11 M, 1 x IO 10 M, 1 x IO 9 M, 1x10 8 M, 1 x IO 7 M, 1 x IO 6 M, 1 x IO 5 M, 1x10 4 M, 1 x IO 3 M, 1 x IO 2 M, 1 x IO 1 M or more of one or more maltodextrins, monosaccharides, disaccharides, sugar alcohols, humic acids, betaines, prolines, sarcosines, peptones, oxidation control components, hygroscopic polymers and / or UV protectants.
[00189] In some embodiments, the inoculant compositions of the present disclosure comprise one or more monosaccharides in an amount / concentration of from about 0.005 to about 50% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about / at least / less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08 , 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25 , 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10 , 15, 20, 25% (by weight) of one or more monosaccharides (eg arabinose, fructose and / or glucose). In some embodiments, one or more monosaccharides are present at a concentration in the range of about 1 x 10 20 M at about 1 x 10' 1 M. For example, one or more monosaccharides may be included at a concentration of about / at least / less than 1 x 10' 20 M, 1 x IO 19 M, 1x10 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10 15 M, 1 x IO 14 M, 1x10 13 M, 1 x IO 12 M, 1x10 11 M, 1 x IO 10 M.
[00190] In some embodiments, the inoculant compositions of the present disclosure comprise one or more disaccharides in an amount / concentration of from about 0.005 to about 50% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about / at least / less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08 , 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25 , 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10 , 15, 20, 25% (by weight) of one or more disaccharides (e.g. maltose, sucrose and / or trehalose). In some embodiments, one or more disaccharides are present at a concentration in the range of about 1 x 10 -20 M at about 1 x 10' 1 M. For example, one or more disaccharides can be included at a concentration of about / at least / less than 1 x 10 20 M, 1x10' 19 M, 1x10 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10 15 M, 1 x IO 14 M, 1x10 13 M, 1x10 12 M, 1x10 11 M, 1x10 10 M.
[00191] In some embodiments, the inoculant compositions of the present disclosure comprise one or more maltodextrins in an amount / concentration of from about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the maltodextrin (or maltodextrins) comprises about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about from 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight ) of the inoculant composition. For example, the inoculant compositions of the present disclosure may comprise about / at least / less than 0.01, 0.02, 0.03, 0.04, 0.05. 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more maltodextrins (for example, one or more maltodextrins (each and / or or collectively) that have a DEV value of about 15 to about 20).
[00192] In some embodiments, the inoculant compositions of the present disclosure comprise one or more sugar alcohols in an amount / concentration of from about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the sugar alcohol (or alcohols) (e.g., arabitol, mannitol, sorbitol and / or xylitol) comprises about 0.001 to about 1%, about 0.25 to about 5%, about 1 about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure may comprise about / at least / less than 0.01, 0.02, 0.03, 0.04, 0.05. 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more sugar alcohols (e.g. arabitol, mannitol, sorbitol and / or xylitol).
[00193] In some embodiments, the inoculant compositions of the present disclosure comprise one or more humic acids in an amount / concentration of from about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the humic acid (or acids) (e.g., potassium humate) comprises about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about from 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure may comprise about / at least / less than 0.01, 0.02, 0.03, 0.04, 0.05. 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more humic acids (eg potassium humate and / or sodium humate).
[00194] In some embodiments, the inoculant compositions of the present disclosure comprise one or more UV protectants in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the UV protectant (or protectants) (e.g., calcium lignosulfate and / or sodium lignosulfate) comprises about 0.0001 to about 0.001, about 0.001 to about 1%, about 0. 25 to about 5% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure may comprise about / at least / less than 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0,05,0,06,0,07, 0,08,0,09,0,1,0,15, 0,2,0,25, 0,3,0,35, 0,4, 0, 45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% or more (by weight) of one or more UV protectants (eg calcium lignosulfate and / or sodium lignosulfate).
[00195] In some embodiments, the inoculant compositions of the present disclosure comprise one or more oxidation control components in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the composition. For example, the inoculant compositions of the present disclosure may comprise about / at least / less than 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0 .04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4 , 0.45, 0.5, 0.75, 1, 1, 25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% of one or more oxidation control components. In some embodiments, the amount / concentration of oxidation control components is from about 0.005 to about 2% (by weight) of the composition. In some embodiments, the oxidation control component (or components) is present at a concentration in the range of about 1 x 10 20 M at about 1 x 10' 1 M. For example, one or more oxidation control components can be added to a concentration of about / at least / less than 1 x 10 -20 M, 1 x IO' 19 M, 1x10' 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10 15 M, 1 x IO 14 M, 1x10 13 M, 1 x IO 12 M, 1x10 11 M, 1x10' 10 M. In some embodiments, the inoculant compositions of the present disclosure comprise one or more commercial antioxidants used in accordance with the amounts / concentrations recommended by the manufacturer. In some embodiments, the inoculant compositions of the present disclosure comprise one or more commercial oxygen scavengers used in the amounts / concentrations recommended by the manufacturer.
[00196] In some embodiments, the inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount / concentration sufficient to ensure that the vegetative cells therein remain viable after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 , 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at -80 °C or less for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 , 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally foliar application and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or greater and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[00197] In some embodiments, the inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount / concentration sufficient to ensure that at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of any vegetative cells in them remain viable after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at -80 °C or less for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally, foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[00198] In some embodiments, the inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount / concentration sufficient to ensure that at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 or more colony forming units of any of the vegetative cells therein remain viable per gram and / or millimeter of inoculant composition after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at -80 °C or less for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally, foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[00199] In some embodiments, the inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount / concentration sufficient to ensure that the relative humidity of deliquescence (DRH) of the inoculant composition is less than 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85 or 90 at the temperature (or temperatures) at which the composition is to be stored (e.g., 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C).
[00200] In some embodiments, the inoculant compositions of the present disclosure comprise two or more stabilizing compounds that synergistically enhance the stability and / or survival of cells vegetation in them.
[00201] The stabilizing compounds may be incorporated into the inoculant compositions of the present disclosure in any suitable ratio (or ratios).
[00202] In some embodiments, the inoculant compositions of the present disclosure comprise one or more maltodextrins and one or more monosaccharides, disaccharides, sugar alcohols and / or humic acids in a ratio of maltodextrin: (monosaccharides, disaccharides, sugar alcohol and / or humic acid) of about 5:95, 10:90, 15:85, 20:80, 25:75, 30:70, 35:65, 40:60, 45:55, 50:50, 55:45 , 60:40, 65:35, 70:30, 75:25, 80:20, 85:15, 90:10, 95:5. For example, the inoculant compositions of the present disclosure can comprise one or more maltodextrins (e.g., one or more maltodextrins (each and / or collectively) having a DEV of about 15 to about 20) and one or more alcohols of sugar (eg, sorbitol and / or xylitol) and / or humic acids (eg, potassium humate) in a maltodextrin:(sugar alcohol / humic acid) ratio of about 5:95, about 15:85 , about 25:75 or about 50:50,
[00203] In some embodiments, the inoculant compositions of the present disclosure comprise one or more biostimulants in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the biostimulant (or biostimulants) (e.g., glycine and / or kelp extract) comprises about about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0, 0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095,0,01,0,015,0,02,0,025,0,03,0,035,0,04,0,045,0,05,0,06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6 , 4.7, 4.8, 4.9, 5% (in weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03 , 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0 .4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1 ,1, 1,2, 1,3, 1,4, 1,5, 1,6, 1,7, 1,8, 1,9, 2, 2,1, 2,2, 2,3, 2 ,4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3 .7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more biostimulants (e.g. glycine and / or kelp extract).
[00204] In some embodiments, the inoculant compositions of the present disclosure comprise one or more microbial extracts in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the microbial extract (or microbial extracts) comprises about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009 , 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.0085 , 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0 .1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8.4.9, 5% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03 , 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0 .4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1 ,1, 1,2, 1,3, 1,4, 1,5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7 , 4.8, 4.9, 5% or more (by weight) of one or more microbial extracts.
[00205] In some embodiments, the inoculant compositions of the present disclosure comprise one or more nutrients in a amount / concentration from about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the nutrient (or nutrients) (e.g., phosphorus, boron, chlorine, copper, iron, manganese, molybdenum, and / or zinc) comprises about 0.0001, 0.0002, 0.0003, 0.0004 ,0.0005,0.0006,0.0007,0.0008,0.0009,0.001,0.0015,0.002,0.0025,0.003,0.0035,0.004,0.0045,0.005,0.0055 , 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0 .05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 , 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8.4.9, 5% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03 , 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0 .4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1 ,1, 1,2, 1,3, 1,4, 1,5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7 , 4.8, 4.9, 5% or more (by weight) of one or more nutrients (eg, phosphorus, boron, chlorine, copper, iron, manganese, molybdenum and / or zinc).
[00206] In some embodiments, the inoculant compositions of the present disclosure comprise one or more pest attractants and / or feeding stimulants in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the composition inoculant. In some embodiments, the pest attractant (or pest attractants) and / or feeding stimulant (or feeding stimulants) comprises about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0 .0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065 ,0,007,0,0075,0,008,0,0085,0,09,0,0095,0,01,0,015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06 , 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5,0.6, 0.7,0,8,0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4.9, 5% (by weight) of inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03 , 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0 .4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1 ,1, 1,2, 1,3, 1,4, 1,5, 1,6, 1,7, 1,8, 1,9, 2, 2,1, 2,2, 2,3, 2 ,4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more pest attractants and / or feeding stimulants.
[00207] In some embodiments, the inoculant compositions of the present disclosure comprise one or more LCOs at a concentration of about 1 x 10 15 M to about 1 x IO' I() M, about 1 x IO 14 M to about 1 x l0' 8 M, about 1 x IO 14 M to about 1 x IO' 6 M, about 1 x IO 12 M to about 1 x l0' 8 M, about 1 x IO 12 M to about 1 x IO' 6 M, about 1 x 10' 10 M to about 1 x 10'6 M, or about I x 10' 8 M to about 1 x 10' 2 M. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10' 20 M, 1 x IO 19 M, 1x10 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10 15 M, 1 x IO 14 M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x IO 10 M, 1 x IO 9 M, 1x10 s M, 1 x IO 7 M, 1 x 10" M, 1 x 10" M, 1 x IO 4 M, 1 x 10" M, 1 x 10" M, 1 x 10 1 M or more of one or more LCOs (eg one, two, three, four or more of the LCOs described above).
[00208] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitin oligosaccharides in a concentration of about 1 x 10 15 M at about 1 x 10’ 10 M, about 1 x IO 14 M to about 1 x l0' 8 M, about 1 x IO 14 M to about 1 x IO' 6 M, about 1 x 10" M about 1 x 10' 8 M, about 1 x IO 12 M to about 1 x IO' 6 M, about 1 x 10' 10 M to about 1 x IO' 6 M, or about I x l0' 8 M to about 1 x IO' 2 M. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10 20 M, 1 x IO' 19 M, 1 x IO' 18 M, 1 x IO 17 M, 1 x IO 16 M, 1 x IO 15 M, 1 x IO 14 M, 1 x IO 13 M, 1 x IO 12 M, 1 x IO 11 M, 1 x IO 10 M, 1 x IO 9 M, 1 x IO 8 M, 1 x IO 7 M, 1 x IO 6 M, 1 x 10" M, 1 x IO 4 M, I x IO 3 M, 1x10' 2 M, 1 x IO' 1 M or more of one or more chitin oligosaccharides (e.g. one, two, three, four or more of the chitin oligosaccharides described above).
[00209] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitosan oligosaccharides in a concentration of about 1 x 10 15 M at about 1 x 10’ 10 M, about 1 x IO 14 M to about 1 x IO' 8 M, about 1 x IO 14 M to about 1 x IO' 6 M, about 1 x IO 12 M to about 1 x 10' 8 M, about 1 x IO 12 M to about 1 x IO' 6 M, about 1 x 10' 10 M to about 1 x IO' 6 M, or about I x l0' 8 M to about 1 x IO' 2 M. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10 20 M, 1 x IO' 19 M, 1 x 10' 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10 15 M, 1 x IO 14 M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x IO 10 M, 1 x IO 9 M, 1x10 8 M, 1 x IO 7 M, 1 x 10" M, 1 x 10" M, 1 x IO 4 M, I x IO 3 M, 1x10' 2 M, 1 x IO' 1 M or more of one or more chitosan oligosaccharides.
[00210] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitins at a concentration of about 1 x 10 15 M to about 1 x IO' I() M, about 1 x IO 14 M to about 1 x l0' 8 M, about 1 x IO 14 M to about 1 x IO' 6 M, about 1 x IO 12 M to about 1 x l0' 8 M, about 1 x IO 12 M to about 1 x IO' 6 M, about 1 x 10' 10 M about I x 10' 6 M or about I x 10' 8 M to about 1 x 10' 2 M. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10” M, 1 x 10 19 M, 1 x IO 18 M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x IO 9 M, 1x10 s M, 1 x IO 7 M, 1 x 10" M, 1 x 10" M, 1 x IO 4 M, 1 x 10" M, 1 x IO 2 m, 1 x IO' 1 M or more of one or more chitins.
[00211] In some embodiments, the inoculant compositions of the present disclosure comprise one or more chitosans in a concentration of about 1 x 10 15 M at about 1 x 10’ 10 M, about 1 x IO 14 M at about 1 x 10’ 8M, about 1 x IO 14 M to about 1 x IO' 6 M, about 1 x IO 12 M to about 1 x IO' 8 M, about 1 x IO 12 M to about 1 x IO' 6 M, about 1 x 10' 10 M to about 1 x IO' 6 M, or about I x IO' 8 M to about 1 x IO' 2 M. For example, the inoculant compositions of the present disclosure can comprise about 1 x 10 20 M, 1 x IO' 19 M, 1 x IO' 18 M, 1 x IO 17 M, 1 x IO 16 M, 1x10 15 M, 1 x IO 14 M, 1 x 10” M, 1 x 10” M, 1 x 10” M, 1 x IO 10 M, 1 x IO 9 M, 1 x IO 8 M, 1 x IO 7 M, 1 x 10” M, 1 x IO 5 M, 1x10 4 M, 1 x IO' 3 M, 1 x IO' 2 M, 1 x IO' 1 M or more of one or more chitosans.
[00212] In some embodiments, the inoculant compositions of the present disclosure comprise one or more flavonoids in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the flavonoid (or flavonoids) (e.g., one or more flavones and / or flavanones) comprises about about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0, 0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007,0.0075,0.008,0.0085,0.009,0.0095,0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8 , 4.9, 5% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03 ,0.04,0.05,0,06,0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0 .4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1 ,1, 1,2, 1,3, 1,4, 1,5, 1,6, 1,7, 1,8, 1,9, 2, 2,1, 2,2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3, 6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more flavonoids (e.g., one or more flavones and / or flavanones).
[00213] In some embodiments, the inoculant compositions of the present disclosure comprise one or more dispersants in an amount / concentration of from about 0.001 to about 25% or more (by weight) of the inoculant composition. In some embodiments, the dispersant (or dispersants) comprises 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0 ,0075, 0,008, 0,0085, 0,009, 0,0095,0,01,0,015,0,02,0,025,0,03,0,035,0,04,0,045,0,05,0,06,0,07 ,0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1 , 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3, 1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4.9 , 5, 6, 7, 8, 9 or 10 to about 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5 , 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.01, 0.02, 0.03, 0.04, 0.05. 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20% or more (in weight) of one or more dispersants (e.g. one or more surfactants and / or wetting agents).
[00214] In some embodiments, the inoculant compositions of the present disclosure comprise one or more drying agents in an amount / concentration of from about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the drying agent (or drying agents) comprises about 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0 .0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0 .06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 , 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2, 7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5 , 4.6, 4.7, 4.8, 4.9, 5, 6, 7, 8, 9 or 10 to about 1, 1.5, 2, 2.5, 3, 3.5, 4 , 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17 , 18, 19 or 20% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.01,0,02,0,03,0,04,0,05,0,06,0,07,0,08,0.09, 0.1 , 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6 ,5,7,7,5,8,8,5,9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more drying (e.g. talc).
[00215] In some embodiments, the inoculant compositions of the present disclosure comprise about 0.5 to about 10 grams of drying powder per liter of inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.5, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 grams or more of drying powder per liter of inoculant composition.
[00216] In some embodiments, the inoculant compositions of the present disclosure comprise one or more buffers in an amount / concentration of from about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the buffer (or buffers) comprises about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001 , 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009.0 ,0095,0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1 , 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3 , 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4 .9.5% (by weight) of the inoculant composition. For example, the inoculant compositions of the present disclosure can comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03 ,0,04,0,05,0,06,0,07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0, 6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1, 5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8 , 4.9, 5% or more (by weight) of one or more buffers (e.g. potassium phosphate monobasic and / or potassium phosphate dibasic).
[00217] In some embodiments, the inoculant compositions of the present disclosure comprise one or more commercial antioxidants, oxygen scavengers, hygroscopic polymers, UV protectors, biostimulants, microbial extracts, nutrients, pest attractants and / or feeding stimulants, pesticides, plant signal molecules, dispersants, drying agents, antifreeze agents, buffers and / or adhesives used in accordance with the manufacturer's recommended amounts / concentrations.
[00218] The inoculant compositions of the present disclosure may be formulated as any suitable type of composition, including, but not limited to, foliar inoculants, seed coatings and soil inoculants.
[00219] In some embodiments, the inoculant compositions of the present disclosure are formulated as non-aqueous formulations in which at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5 , 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of the microbial cells / spores on them survive when the composition inoculant is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 , 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72 ,76, 80, 84, 88, 92, 96, 100, 104 weeks or more. In some embodiments, the inoculant compositions of the present disclosure are formulated so that at least about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70% or more of the microorganisms in them survive when the inoculant composition is coated onto a seed and stored at 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30 °C and 50, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00220] In some embodiments, the inoculant compositions of the present disclosure are formulated as non-aqueous formulations in which at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 cfu / gram or more of the microbial cells / spores therein survive when the inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40 , 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00221] In some embodiments, the inoculant compositions of the present disclosure are formulated as non-aqueous formulations in which at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 cfu / ml or more of the microbial cells / spores therein survive when the inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40 , 44, 48, 52, 56, 60, 64, 68, 12, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00222] In some embodiments, the inoculant compositions of the present disclosure are formulated as non-aqueous formulations in which at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 cfu / seed or more of the microorganisms therein survive when the inoculant composition is coated onto a seed and stored at 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30°C and 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68 , 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00223] In some embodiments, the inoculant compositions of the present disclosure are formulated as amorphous liquids.
[00224] In some embodiments, the inoculant compositions of the present disclosure do not comprise water.
[00225] In some embodiments, the inoculant compositions of the present disclosure do not comprise a trace amount of water.
[00226] In some embodiments, the inoculant compositions of the present disclosure comprise less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08.0, 09,0,1,0,15,0,2,0,25,0,3,0,35,0,4, 0.45, 0.5% water by weight based on the total weight of the composition .
[00227] In some embodiments, the inoculant compositions of the present disclosure are formulated to have a pH of about 4.5 to about 9.5. In some embodiments, the inoculant compositions of the present disclosure have a pH of from about 6 to about 7.5. In some embodiments, the inoculant compositions of the present disclosure have a pH of about 5, 5.5, 6, 6.5, 7, 7.5, 8 or 8.5.
[00228] It should be understood that the inoculant compositions of the present disclosure are non-naturally occurring compositions. According In some embodiments, the inoculant composition comprises one or more non-naturally occurring components. According to some embodiments, the inoculant composition comprises a non-naturally occurring combination of naturally occurring components.
[00229] The inoculant compositions of the present disclosure exhibit several properties, including, but not limited to, the ability to enhance both the stability and survival of microbial cells / spores therein.
[00230] In some embodiments, the inoculant compositions of the present disclosure improve the stability of one or more microbial cells / spores contained therein by at least about 0.1, 0.5, 1, 2, 3.4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or greater compared to a control composition (e.g., a control composition that is identical to the inoculant composition of the present disclosure, except that it lacks one or more components found in the inoculant composition and / or contains one or more components not found in the inoculant composition of the present disclosure). For example, the inoculant compositions of the present disclosure can improve one or more microbial stability characteristics of one or more of the microbial cells / spores contained therein by at least about 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more compared to a control composition which is identical to the inoculant composition except that it lacks one or more of the paraffin oils and / or waxes and / or comprises a reduced amount of one or more of the paraffin oils and / or waxes.
[00231] In some embodiments, microbial cells / spores remain viable in the inoculant compositions of the present disclosure for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 , 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more (e.g., at least 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more when stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity).
[00232] In some embodiments, the inoculant compositions of the present disclosure improve the survival rate of one or more microbial cells / spores contained therein by at least about 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or greater compared to a control composition (e.g., a control composition that is identical to the inoculant composition of the present disclosure, except that it lacks one or more of the components found in the inoculant composition and / or contains one or more components not found in the inoculant composition of the present disclosure). For example, the inoculant compositions of the present disclosure can improve the survival rate of one or more of the microbial cells / spores contained therein by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more compared to a control composition that is identical to the inoculant composition, except that it lacks one or more of the paraffin oils and / or waxes and / or comprises a minor amount of one or more of the paraffin oils and / or waxes.
[00233] In some embodiments, the inoculant compositions of the present disclosure improve the survival of one or more of the microbial cells / spores contained therein to the point where at least about 0.01, 0.05, 0.1, 0 ,5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of microbial cells / spores survive when the inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or most. In some embodiments, the inoculant compositions of the present disclosure improve the survival of one or more of the microbial cells / spores contained therein to the point where at least about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70% or more of microbial cells / spores survive when inoculant composition is stored at 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30°C for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00234] In some embodiments, the inoculant compositions of the present disclosure improve the survival of one or more of the microbial cells / spores contained therein to the point where at least about 0.01, 0.05, 0.1, 0 ,5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of the microbial cells / spores survive when the inoculant composition is dried at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and stored at a 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3 , 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72,76, 80, 84, 88, 92, 96, 100, 104 weeks or more. In some embodiments, the inoculant compositions of the present disclosure enhance the survival of one or more of the microbial cells / spores contained therein until the point at which at least about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70% or more microbial cells / spores survive when the inoculant composition is coated onto a seed, dried and stored at 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30°C and 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22 , 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00235] In some embodiments, the inoculant compositions of the present disclosure improve the survival of one or more of the microbial cells / spores contained therein to the point where at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 cfu / gram or milliliter or more of the microbial cells / spores survive when the inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C for a period of 1.2, 3.4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more. In some embodiments, the inoculant compositions of the present disclosure enhance the survival of one or more of the microbial cells / spores contained therein to the point where at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 cfu / seed or more of the microbial cells / spores survive when the inoculant composition is stored at 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30° for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00236] In some embodiments, the inoculant compositions of the present disclosure improve the survival of one or more of the microbial cells / spores contained therein to the extent that at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 , 1 x 10 11 , 1 x 10 12 cfu / gram or milliliter or more of the microbial cells / spores survive when the inoculant composition is dried to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or higher and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period from 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32 , 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more. In some embodiments, the inoculant compositions of the present disclosure enhance the survival of one or more of the microbial cells / spores contained therein to the point where at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 cfu / seed or more of the microbial cells / spores survive when the inoculant composition is coated onto a seed, dried and stored at 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 , 23, 24, 25, 26, 27, 28, 29 and / or 30°C and 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00237] In some embodiments, the inoculant compositions of the present disclosure improve both the survival rate and one, two, three, four, five, six, seven, eight, nine, ten or more microbial stability characteristics of the spore (or spores ) microbial content contained therein.
[00238] In some embodiments, the inoculant compositions of the present disclosure improve the dispersion of one or more microbial cells / spores contained therein by at least about 0.1, 0.5, 1, 2, 3.4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or greater compared to a control composition (e.g., a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition and / or contains a or more components not found in the inoculant composition of the present disclosure). For example, the inoculant compositions of the present disclosure can improve the dispersion of one or more of the microbial cells / spores contained therein by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more compared to a control composition that is identical to the inoculant composition, except that lacks one or more of the paraffin oils and / or waxes and / or comprises a minor amount of one or more of the paraffin oils and / or waxes.
[00239] In some embodiments, the inoculant compositions of the present disclosure improve the dispersal of one or more of the microbial cells / spores contained therein to the point where at least about 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more of the microbial cells / spores are present as single cells / spores (rather than as members of a cluster comprising two or more cells / spores).
[00240] The inoculant compositions of the present disclosure can be used to improve any suitable microbial stability trait (or suitable microbial stability traits), including, but not limited to, the ability of the microbial cells / spores therein to enhance plant yield after being coated in a seed and stored for a defined period of time before planting the seed. For example, in some embodiments, the inoculant compositions of the present disclosure improve the ability of microbial cells / spores therein to propagate and increase yield after being coated in a plant propagation material (eg seed) and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 , 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00241] As noted above, the inoculant compositions of the present disclosure may comprise agriculturally beneficial constituents such as biostimulants, microbial extracts, nutrients, pesticides and plant signal molecules. It is to be understood that agriculturally beneficial constituents can also be used in conjunction with the inoculant compositions of the present disclosure. Thus, the present disclosure extends to systems and methods of using the inoculant compositions of the present disclosure in conjunction with compositions comprising one or more agriculturally beneficial constituents (e.g., a second composition comprising one or more LCOs and / or chitin oligomers , a third composition comprising one or more fungicides, herbicides, insecticides and / or nematicides, etc.).
[00242] The present disclosure extends to kits comprising, consisting essentially of, or consisting of two or more containers, each comprising one or more components of an inoculant composition of the present disclosure. For example, the microbial cells / spores and carrier can be housed in separate containers for long-term storage, then combined prior to application of the inoculant composition to the target medium (e.g., a plant or plant propagation material). Optional constituents such as stabilizing compounds, pesticides and plant signaling molecules can be added to either of the two containers or housed in one or more separate containers to long-term storage. In some embodiments, the kit further comprises one or more oxygen scavengers, such as activated carbon, ascorbic acid, powdered iron, ferrous carbonate mixtures, and metal halide, sodium chloride, and / or sodium hydrogen carbonate catalysts.
[00243] The containers may comprise any suitable material (or materials), including, but not limited to, materials that reduce the amount of light, moisture and / or oxygen that comes into contact with the plant propagation material when the container is sealed . In some embodiments, the containers comprise, consist essentially of, or consist of a material that has a light permeability of less than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60, 65, 70 or 75%. In some embodiments, the containers comprise, consist essentially of, or consist of a material that has an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475 or 500 cm 3 / m 2 -day (as measured in accordance with ASTM D3985).
[00244] In some embodiments, the containers reduce the amount of ambient light, moisture and / or oxygen reaching said coated plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
[00245] The inoculant compositions of the present disclosure can be applied to any type of plant, including, but not limited to, crops and row vegetables. In some embodiments, the inoculant compositions of the present disclosure are formulated to treat one or more plants selected from the Amaranthaceae (e.g. Swiss chard, spinach, sugar beet, quinoa), Asteraceae (e.g. artichoke, aster, chamomile, chicory, chrysanthemum, dahlias, daisies, echinacea, solidago, gaiule, lettuce, marigolds, saffron, sunflowers, zinnia), Brassicaceae (e.g. arugula, broccoli, Chinese cabbage, Brussels sprouts, cabbage, cauliflower, canola, collard greens, white radish, garden cress , horseradish, kale, mustard, radish, rapeseed, turnip, turnip, wasabi, watercress), Arabidopsis thaliana, Cucurbitaceae (e.g. cantaloupe, cucumber, honeydew melon, cantaloupe, pumpkin, pumpkin (e.g. acorn squash, butternut squash, summer squash), watermelon, summer squash), Fabaceae (e.g., alfalfa, beans, carob, clover, guar, lentils, mesquite, peas, peanuts, soybeans, tamarind, tragacanth, vicia), Malvaceae (e.g. cocoa, cotton, durium, hibiscus, kenaf, rye, okra), Poaceae (e.g. bamboo, barley, maize, fonio, grass (e.g. bay grass, bermudagrass, bluegrass, guinea fowl, centipede grass, fescue or Zoysia), millet, oats, ornamental grasses, rice, rye, sorghum, sugar cane, triticale, wheat and other crops cereals), Polygonaceae (e.g. buckwheat), Rosaceae (e.g. almonds, apples, apricots, cloudberries, blueberries, cherries, peaches, plums, quinces, raspberries, roses, strawberries), Solanaceae (e.g. peppers , chili pepper, eggplant, petunia, potato, tobacco, tomato), Vitaceae (e.g., grape) In some embodiments, the inoculant compositions of the present disclosure are formulated for treatment of one or more plants with which microbial cells / spores are not naturally associated (e.g. one or more plants that do not naturally exist in the geographic location (or geographic locations) where the microbial cells / spores naturally exist). In some embodiments, the inoculant compositions of the present disclosure are formulated for treating one or more acaricide, fungicide, gastropodicide, herbicide, insecticide, nematicide, rodenticide, and / or virucide resistant plants (e.g., one or more inhibitor resistant plants). acetolactate synthase (e.g. imidazolinone, priimidinoxy(thio)benzoates, sulfonylaminocarbonyltriazolinone, sulfonylurea, triazolopyrimidines), bialaphos, glufosinate, glyphosate, hydroxyphenylpyruvate disoxygenase inhibitors and / or phosphinothricin). Non-limiting examples of plants that can be treated with inoculant compositions of the present disclosure include those plants sold by the Monsanto Company (St. Louis, MO) under the tradenames BOLLGARDII®, DROUGHTGARD®, GENUITY®, INTACTA, INTACTA RR2 PRO, RIB COMPLETE ®, ROUNDUP READY®, ROUNDUP READY 2 RENDIMENTO®, ROUNDUP READY 2 EXTEND™, SMARTSTAX®, VT DOUBLE PRO®, VT TRIPLE PRO®, YIELDGARD®, YIELDGARD VT R00TW0RM / RR2®, YIELDGARD VT TRIPLE® and / or XTENDFLEX™ .
[00246] The inoculant compositions of the present disclosure can be applied to any part / portion of a plant. In some embodiments, the inoculant compositions of the present disclosure are formulated for the treatment of plant propagation materials (e.g., cuttings, rhizomes, seeds and tubers). In some embodiments, the inoculant compositions of the present disclosure are formulated for the treatment of plant roots. In some embodiments, the inoculant compositions of the present disclosure are formulated for the treatment of plant foliage. In some embodiments, the inoculant compositions of the present disclosure are formulated to treat both the roots and the foliage of a plant. In some embodiments, the inoculant compositions of the present disclosure are formulated for the treatment of plant propagation materials and the plants that grow from said plant propagation materials.
[00247] The inoculant compositions of the present disclosure can be applied to any plant growth medium, including, but not limited to, soil.
[00248] The inoculant compositions of the present disclosure can be applied to plants, plant parts and / or plant growth media in any suitable manner, including, but not limited to, application to seed, furrow application and foliar application.
[00249] The inoculant compositions of the present disclosure can be applied using any suitable method (or methods), including, but not limited to, coating, dripping, spraying, encapsulating, dipping, sprinkling and soaking. Batch systems, in which predetermined batch sizes of material and inoculant composition are delivered to a mixer, can be employed. Continuous treatment systems, which are calibrated to apply the inoculant composition at a predefined rate in proportion to a continuous flow of material, can also be employed.
[00250] In some embodiments, the inoculant compositions of the present disclosure are applied directly to plant propagation material (e.g., seeds). According to some embodiments, plant propagation materials are soaked in an inoculant composition of the present disclosure for at least 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0, 7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 3, 4, 5, 6, 9, 12, 15, 18, 21, 24, 36, 48 hours . According to some embodiments, plant propagation materials are coated with an inoculant composition of the present disclosure. The plant propagation materials may be coated with one or more additional layers (e.g., one or more protective layers which serve to further enhance the stability and / or survival of microbial spores and / or vegetative cells in the inoculant composition and / or one or more scavenging layers comprising substances that may reduce the stability and / or survival of microbial spores and / or vegetative cells in the inoculating composition if included in the same layer as said microbial spores and / or vegetative cells). In some embodiments, the coating comprises, consists essentially of, or consists of an inoculant composition of the present invention and a drying powder.
[00251] In some embodiments, the inoculant compositions of the present disclosure are applied directly to a growth medium of plant (for example, a soil). According to some embodiments, the inoculant compositions of the present disclosure are applied to the vicinity of a plant propagation material (e.g., a seed). According to some embodiments, the inoculant compositions of the present disclosure are applied to the root zone of a plant. According to some embodiments, the inoculant compositions of the present disclosure are applied using a drip irrigation system.
[00252] In some embodiments, the inoculant compositions of the present disclosure are applied directly to the plants. According to some embodiments, the inoculant compositions of the present disclosure are sprayed and / or sprinkled on the plant (or plants) to be treated.
[00253] In some embodiments, the inoculant compositions of the present disclosure are freeze and spray dried or spray and freeze dried and then applied to the plants / plant parts. For example, in some embodiments, an inoculant composition comprising one or more paraffin oils and / or waxes, as well as one or more stabilizing components (e.g., one or more maltodextrins having a DEV of about 15 to about 20 ) is freeze-dried, spray-dried or spray-freeze dried, mixed with a drying powder (e.g. a drying powder comprising calcium stearate, attapulgite clay, montmorillonite clay, graphite, magnesium stearate, silica (e.g. pyrolysed silica, hydrophobically coated silica and / or precipitated silica) and / or talc), then coated onto seed which has been pre-treated with one or more adhesives (e.g. an adhesive composition comprising one or more maltodextrins, one or more mono, di or oligosaccharides, one or more peptones, etc.), one or more pesticides and / or one or more plant signaling molecules (e.g. one or more LCOs).
[00254] The inoculant compositions of the present disclosure can be applied to plants, plant parts and / or plant growth media in any suitable amount (or amounts) / concentration (or concentrations).
[00255] In some embodiments, the inoculant compositions of the present disclosure are applied at a rate of from about 0.05 to about 100 millimeters and / or grams of inoculant composition per kilogram of plant propagation material. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that the plant propagation materials are coated with about / at least 0.05, 0.1, 0.125, 0.15 0.175 0.2 0.225 0.2.5 0.275 0.3 0.325 0.35 0.375 0.4 0.425 0.45 0.475 0.5 0.55 0 .6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25 , 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and / or grams of inoculant compositions per kilogram of plant propagation material. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that a measure of about / at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2 , 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65 , 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2 .75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters and / or grams of inoculant composition is applied to each seed.
[00256] In some embodiments, the inoculant compositions of the present disclosure are applied at a rate of from about 0.5 to about 100 millimeters and / or grams of inoculant composition per plant. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that each plant is treated with about / at least 0.05, 0.1, 0.125, 0.15, 0.175, 0 .2, 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0 .65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5 , 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and / or grams of inoculant composition. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2 , 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65 , 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters and / or grams of inoculant composition is applied to each plant.
[00257] In some embodiments, the inoculant compositions of the present disclosure are applied at a rate of from about 0.5 to about 100 millimeters and / or grams of inoculant composition per acre of treated crops. In accordance with some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that each acre of treated crops is treated with about / at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0, 6, 0.65, 0, 7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and / or grams of inoculant composition. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 0.05, 0.1, 0.125, 0.15, 0.175, 0, 2, 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0, 65,0,7,0,75, 0,8,0,85,0,9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters and / or grams of inoculant composition is applied to each acre of treated crops.
[00258] In some embodiments, the inoculant compositions of the present disclosure are applied at a rate of from about 0.5 to about 100 millimeters and / or grams of inoculant composition per acre of plant growth media. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that each acre of plant growth media is treated with about / at least 0.05, 0.1, 0.125, 0 .15, 0.175, 0.2, 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55 , 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2 .25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and / or grams of inoculant composition. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2 , 0.225, 0.2.5, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65 , 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, or 5 milliliters and / or grams of inoculant composition is applied to each acre of growth media of plant.
[00259] In some embodiments, the inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that the plant propagation materials are coated with about / at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores per kilogram of plant propagation material. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores is applied to each seed.
[00260] In some embodiments, the inoculant compositions of present disclosure are applied in an amount sufficient to ensure that each plant is treated with about / at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x IO 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores is applied to each plant.
[00261] In some embodiments, the inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that each acre of treated crops is treated with about / at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x 10 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores is applied to every acre of treated crops.
[00262] In some embodiments, the inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that each acre of plant growth media is treated with about / at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 , 1x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores. According to some embodiments, one or more inoculant compositions of the present disclosure are applied in an amount sufficient to ensure that an average of about / at least 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 microbial cells / spores is applied to each acre of growth media of plant.
[00263] The inoculant compositions of the present disclosure can be applied to plants, plant parts and / or plant growth media at any time, including, but not limited to, before planting, at the time of planting, after planting, before of germination, at the time of germination, after germination, before seedling emergence, at the time of seedling emergence, after seedling emergence, before the vegetative stage, during the vegetative stage, after the vegetative stage, before the reproductive stage , during the reproductive stage, after the reproductive stage, before flowering, at the time of flowering, after flowering, before fruit set, at the time of fruit set, after fruit set, before decay, at the time of decay and after decay. In some embodiments, an inoculant composition of the present disclosure is applied to plant propagation materials (e.g., seeds) about / at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72 , 76, 80, 84, 88, 92, 96, 100, 104 weeks before planting.
[00264] In some embodiments, an inoculant composition of the present disclosure is applied to plant propagation materials (eg, seeds) at the time of planting.
[00265] In some embodiments, an inoculant composition of the present disclosure is applied to plant propagation materials (eg, seeds) after planting, but prior to germination.
[00266] In some embodiments, an inoculant composition of the present disclosure is applied to plants after emergence.
[00267] The present disclosure extends to plants and plant parts (e.g., coated plant propagation materials) that have been treated with an inoculant composition of the present disclosure, to plants growing from plant parts (e.g., plant propagation materials coated) that have been treated with an inoculant composition of the present disclosure, to plant parts harvested from plants that have been treated with an inoculant composition of the present disclosure, to plant parts harvested from plants growing from plant parts (e.g., coated plant propagation materials) that have been treated with an inoculant composition of the present disclosure, to processed products derived from plants that have been treated with an inoculant composition of the present disclosure, to processed products derived from plants that have grown from plant parts (e.g., coated plant propagation materials) that have been treated with an inoculating composition of the present disclosure, to cultures comprising a plurality of plants that have been treated with an inoculating composition of the present disclosure, and to cultures comprising a plurality of plants that grow from plant parts (e.g. ie, coated plant propagation materials) which have been treated with an inoculant composition of the present disclosure.
[00268] In some embodiments, the present disclosure provides coated plant propagation materials comprising, consisting essentially of or consisting of a plant propagation material and a coating that coats at least a portion of the outer surface of the plant propagation material , wherein said coating comprises, essentially consists of or consists of an inoculant composition of the present disclosure.
[00269] In some embodiments, the coating comprises two, three, four, five or more layers. According to some embodiments, the coating comprises an inner layer containing an inoculating composition of the present disclosure and one or more outer layers free or substantially free of microorganisms. In some embodiments, the coating comprises an inner layer that is an inoculant composition of the present disclosure and an outer layer that is equivalent to an inoculant composition of the present disclosure, except that it does not contain microbial cells / spores.
[00270] In some embodiments, the coating comprises, essentially consists of or consists of an inoculant composition of the present invention and a drying powder. Drying powders can be applied in any suitable amount / concentration (or suitable amounts / concentrations). The absolute value of the amount / concentration that is sufficient to cause the desired effect (or effects) can be affected by factors such as the type, size and volume of material to which the composition will be applied, the type (or types) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition, and storage conditions (eg, temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount / concentration using routine dose response experiments after studying the present disclosure. Guidance for selecting appropriate amounts / concentrations can be found, for example, in International Patent Applications Nos. PCT / US2016 / 050529 and PCT / US2016 / 050647 and US Provisional Patent Applications 62 / 296,798; 62 / 271,857; 62 / 347,773; 62 / 343,217; 62 / 296,784; 62 / 271,873; 62 / 347,785; 62 / 347,794; and 62 / 347,805. In some embodiments, the drying powder is applied in an amount ranging from about 0.5 to about 10 grams of drying powder per kilogram of plant propagation material. For example, in some embodiments, about 0.5, 1, 1.25,1,5,1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 grams or more of drying powder (for example, drying powder comprising magnesium stearate, magnesium sulfate, milk powder, silica and / or talc) is applied by kilogram of seed. In some embodiments, a drying powder comprising calcium stearate, attapulgite aryl, montmorillonite clay, graphite, magnesium stearate, silica (e.g., silica pyrolyzed silica, hydrophobically coated silica and / or precipitated silica) and / or talc is applied to seeds coated with an inoculant composition of the present disclosure at a rate of about 1, 1.25, 1.5, 1.75, 2, 2 .25, 2.5, 2.75 or 3 grams per kilogram of seed.
[00271] In some embodiments, the coating completely covers the outer surface of the plant propagation material.
[00272] In some embodiments, the average coating thickness is at least 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3 , 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 4 , 4.5, 5 pm or so. In some embodiments, the average coating thickness is about 1.5 to about 3.0 µm.
[00273] The present disclosure extends to kits comprising, consisting essentially of, or consisting of one or more plants and / or plant parts (e.g., coated plant propagation materials) that have been treated with or are an inoculant composition of the present disclosure and a container that houses the treated plant (or plants) and / or plant part (or parts). In some embodiments, the kit further comprises one or more oxygen scavengers, such as activated carbon, ascorbic acid, powdered iron, ferrous carbonate mixtures, and metal halide, sodium chloride, and / or sodium hydrogen carbonate catalysts.
[00274] The container may comprise any suitable material (or suitable materials), including, but not limited to, materials that reduce the amount of light, moisture and / or oxygen that contact the plant propagation material when the container is tight. In some embodiments, the container comprises, consists essentially of, or consists of a material that has a light permeability of less than about 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 or 75%. In some embodiments, the container comprises, consists essentially of, or consists of a material having an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70 , 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 cm 3 / m 2 -day (as measured in accordance with ASTM D3985).
[00275] In some embodiments, the container reduces the amount of ambient light, moisture and / or oxygen reaching said coated plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
[00276] In some embodiments, kits of the present disclosure comprise 1, 2, 3, 4, 5 or more additional containers. Additional containers can comprise any suitable component or composition (or suitable components or compositions), including, but not limited to, agriculturally beneficial microorganisms, biostimulants, drying agents, nutrients, oxidation control components, and pesticides. Examples of agriculturally beneficial microorganisms, biostimulants, drying agents, nutrients, oxidation control components and pesticides that can be included in additional containers are described above.
[00277] The present disclosure extends to animal feed compositions comprising, consisting essentially of or consisting of a food component and a microbial component, wherein said microbial component comprises, essentially consists of or consists of an inoculant composition of present revelation.
[00278] The animal feed compositions of the present disclosure may comprise any suitable feed component, including, but not limited to, sawdust (e.g. grain, hay, legumes, silage and / or straw) and forage (e.g. grass ).
[00279] Any suitable animal can be fed the animal feed compositions of the present invention, including, but not limited to, farm animals, zoo animals, laboratory animals and / or companion animals. In some embodiments, the animal feed composition is formulated to meet the dietary needs of birds (e.g. chickens, ducks, quails and / or turkeys), cattle (eg antelopes, bison, cattle, gazelles, goats, impala, oxen, sheep and / or wildebeests), dogs, deer (eg caribou, deer, reindeer and / or moose), equine (e.g. donkeys, horses and / or zebras), cats, fish, pigs, rabbits, rodents (e.g. guinea pigs, hamsters, mice and / or rats) and the like .
[00280] The present disclosure extends to methods and uses for inoculant compositions of the present disclosure.
[00281] In some embodiments, the methods and uses of the present disclosure comprise, essentially consist of or consist of applying an inoculant composition of the present disclosure to a plant or plant part (e.g., plant propagation material). As noted above, the inoculant compositions of the present disclosure can be applied to any type of plant, to any part / portion of a plant, in any suitable manner, in any suitable amount (or amounts) / concentration (or concentrations) and at any suitable time (or times) In some embodiments, the methods and uses of the present disclosure comprise, essentially consist of, or consist of applying an inoculant composition of the present disclosure to a monocot plant or plant part (e.g., a cereal plant or from pseudocereal or plant part, optionally barley, buckwheat, maize, millet, oats, quinoa, rice, rye, sorghum or wheat). In accordance with some embodiments, the methods and uses of the present disclosure comprise, essentially consist of, or consist of applying an inoculant composition of the present disclosure to a dicotyledonous plant or plant part (e.g., a leguminous plant or plant part, optionally, alfalfa, beans, lentils, peas, peanuts or soybeans).
[00282] In some embodiments, the methods and uses of the present disclosure comprise, consist essentially of or consist of applying an inoculant composition of the present disclosure to a plant growth medium. As noted above, the inoculant compositions of the present disclosure can be applied to any plant growth medium, in any suitable manner, in any suitable amount (or amounts) / concentration (or concentrations) and at any suitable time (or times).
[00283] In some embodiments, the methods and uses of the present disclosure comprise, essentially consist of, or consist of introducing a plant or plant part (e.g., plant propagation material) that has been treated with an inoculant composition of the present disclosure into a plant growing medium (eg a soil). Such methods may further comprise introducing one or more nutrients (eg nitrogen and / or phosphorus) into the plant growth medium. Any suitable nutrient (or nutrients) can be added to the growth medium, including, but not limited to, rock phosphate, monoammonium phosphate, diammonium phosphate, monocalcium phosphate, superphosphate, triple superphosphate, ammonium polyphosphate, fertilizers comprising one or more sources of phosphorus and combinations thereof.
[00284] In some embodiments, methods and uses of the present disclosure comprise, essentially consist of or consist of cultivating a plant from a plant propagation material that has been treated with an inoculant composition of the present disclosure.
[00285] The present disclosure extends to methods for improving the stability and / or survival of microbial cells / spores in a composition, said methods comprising, consisting essentially of or consisting of adding one or more oils and / or paraffin waxes to said composition in an amount effective to enhance the stability and / or survival of microbial cells / spores therein.
[00286] Paraffin oils and / or waxes can be used to improve any suitable microbial stability characteristics (or suitable microbial stability characteristics) of the microbial cells / spores in a composition, including, but not limited to, the ability of the microbial cells / spores in a composition to enhance plant yield after being coated in a seed and stored for a defined period before planting the seed. For example, in some embodiments, the addition of one or more paraffin oils and / or waxes to a composition enhances the ability of microbial cells / spores therein to propagate and increase yield after being coated onto a plant propagation material ( e.g. seed) and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8 , 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64 , 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00287] In some embodiments, adding one or more paraffin oils and / or waxes to a composition improves the stability of one or more microbial cells / spores therein by at least about 0.1, 0.5, 1 , 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110 , 115, 120, 125, 150, 175, 200% or more compared to a control composition (e.g., a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition and / or contains one or more components not found in the inoculant composition of the present disclosure). For example, the addition of one or more paraffin oils and / or waxes to a composition can improve the survival rate of one or more of the microbial cells / spores contained therein by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more compared to a control composition that is identical to the inoculant composition except that it lacks one or more oils and / or paraffin waxes and / or or comprises a reduced amount of one or more paraffin oils and / or waxes.
[00288] In some embodiments, the addition of one or more paraffin oils and / or waxes to a composition improves the survival of one or more of the microbial cells / spores in an inoculant composition to the point where at least about 0, 01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of microbial cells / spores survive when inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 , 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37 , 38, 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more. In some embodiments, the addition of one or more paraffin oils and / or waxes to a composition improves the survival of one or more of the microbial cells / spores in an inoculant composition to the point where at least about 5, 6, 7 , 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70% or more Microbial cells / spores survive when the inoculant composition is coated onto a seed, dried and stored at 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30°C and 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1,2,3,4,5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00289] In some embodiments, the addition of one or more paraffin oils and / or waxes to a composition improves the survival of one or more of the microbial cells / spores in an inoculant composition to the point where at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6, 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1x IO 10 , 1x IO 11 , 1 x 10 12 cfu / seed or more of the microbial cells / spores survive when the inoculant composition is coated onto a seed and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 , 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 , 39 and / or 40°C and 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more. In some embodiments, the addition of one or more paraffin oils and / or waxes to a composition improves the survival of one or more of the microbial cells / spores in an inoculant composition to the point where at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 cfu / seed or more of the microbial cells / spores survive when the inoculant composition is coated onto a seed and stored at 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and / or 30°C and 30°C , 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00290] In some embodiments, adding one or more paraffin oils and / or waxes to a composition improves both the survival rate and one, two, three, four, five, six, seven, eight, nine, ten or more microbial stability characteristics of the microbial spore (or microbial spores) contained therein.
[00291] The absolute value of the amount / concentration / dosage of one or more oils and / or paraffin waxes that must be added to the composition to improve the stability and / or survival of microbial cells / spores in it can be affected by factors, such as the type, size and volume of the composition, the inherent stability of the microbial cells / spores in the composition, identity and amounts / concentrations of other components in the inoculant composition (eg monosaccharides, disaccharides, sugar alcohols, oxidation control components) and storage conditions (eg temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount / concentration / dosage using routine dose response experiments after studying the present disclosure.
[00292] In some embodiments, one or more of the paraffin oils and / or waxes are added to the composition until it comprises about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15 , 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99, 99.5% or more (by weight ) of said composition, optionally about 1 to about 99%, about 5 to about 95%, about 10 to about 95%, about 15 to about 95%, about 20 to about 95 %, about 25 to about 95%, about 30 to about 95%, about 35 to about 95%, about 40 to about 95%, about 45 to about 95%, about 50 to about 95%, about 55% to about 95%, about 60% to about 95%, about 65% to about 95%, about 70 to about 95%, about 75 to about about 95%, about 80 to about 95% or about 80 to about 95%, about 5 to about 90%, about 10 to about 90%, about 15 to about 90%, about from 20 to about 90%, about 25 to about 90%, about 30 to about 90%, about 35 about 90%, about 40 to about 90%, about 45 to about 90%, about 50 to about 90%, about 55% to about 90%, about 60% to about 90% 90%, about 65% to about 90%, about 70 to about 90%, about 75 to about 90%, about 80 to about 90%, or about 80 to about 90% (in weight) of said composition.
[00293] In some embodiments, a combination of two, three, four or more paraffin oils and / or waxes is added to the composition until it comprises about 1, 2, 3, 4, 5, 6, 7, 8 , 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99, 99.5 % or more (in weight) of said composition, optionally about 1 to about 99%, about 5 to about 95%, about 10 to about 95%, about 15 to about 95%, about 20 to about 95%, about 25 to about 95%, about 30 to about 95%, about 35 to about 95%, about 40 to about 95%, about 45 to about 95%, about 50 to about 95%, about 55% to about 95%, about 60% to about 95%, about 65% to about 95%, about 70 to about 95%, about 75 to about 95%, about 80 to about 95% or about 80 to about 95%, about 5 to about 90%, about 10 to about 90%, about 15 to about 90%, about 20 to about 90%, about 25 to about 90%, about 30 to about 90%, about 35 to about 90%, about 40 to about 90%, about 45 to about about 90%, about 50 to about 90%, about 55% to about 90%, about 60% to about 90%, about 65% to about 90%, about 70 to about 90 %, about 75 to about 90%, about 80 to about 90%, or about 80 to about 90% (by weight) of said composition.
[00294] The paraffin oils and / or waxes can be added to the composition in any suitable ratio (or suitable ratios). In some embodiments, two, three, four or more paraffin oils and / or waxes are added to the composition in one of the ratios described above in relation to the inoculant compositions of the present disclosure.
[00295] In some embodiments, one or more oils and / or paraffin waxes are added to the composition in an amount / concentration sufficient to ensure that the microbial cells / spores remain viable in the composition after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below -80 °C for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally, foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1 , 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[00296] In some embodiments, one or more paraffin oils and / or waxes are added to the composition in an amount / concentration sufficient to ensure that at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of microbial cells / spores in remain viable after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1.2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 , 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92 , 96, 100, 104 weeks or more; cryopreservation at or below -80 °C for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally, foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[00297] In some embodiments, one or more paraffin oils and / or waxes are added to the composition in an amount / concentration sufficient to ensure that at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 or more colony forming units of the microbial cells / spores in it remain viable per gram and / or milliliter of composition after: storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0.1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40 , 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below -80 °C for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; application to plant propagation material (optionally seed), optionally application to plant propagation material and drying in about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95% or more and / or application to a plant propagation material and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 , 35, 36, 37, 38, 39 and / or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; and / or foliar application, optionally, foliar application and desiccation at about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or greater and / or foliar application and exposure to temperatures of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and relative humidities of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95% or more for a period of 0.1, 0.2, 0.25, 0.5, 0.75, 1, 1.5 , 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or more.
[00298] In some embodiments, two, three, four or more paraffin oils and / or waxes are added to the composition in amounts / concentrations sufficient to synergistically enhance the stability and / or survival of microbial cells / spores therein.
[00299] The present invention also provides systems and methods for using inoculant compositions of the present invention in conjunction with additional compositions comprising one or more agriculturally beneficial constituents. The further composition (or compositions) may comprise any suitable agriculturally beneficial constituent (or constituents), including, but not limited to, the agriculturally beneficial ones described above.
[00300] In some embodiments, the inoculant compositions of the present invention are used in conjunction with one or more seed compositions, one or more furrow compositions, and / or one or more leaf-applied compositions.
[00301] In some embodiments, the inoculant compositions of the present invention are used as part of an integrated disease and / or pest control system.
[00302] The specific embodiments of the present disclosure are described in the following numbered paragraphs: 1. An inoculant composition comprising, consisting essentially of or consisting of: microbial cells / spores and a carrier comprising, consisting essentially of or consisting of one or more oils and / or paraffin waxes.
[00303] 2. The inoculant composition, according to any of the preceding paragraphs, said microbial cells / spores comprising about 0.1% to about 50% (by weight) of said inoculant composition, optionally about 5 to about 15% (by weight) of said composition , optionally about 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 , 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48 , 49 or 50% (by weight) of said inoculant composition.
[00304] 3. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores being present in said inoculant composition in a concentration in the range of about 1 x 10 1 at about 1 x IO 20 colony forming units per gram and / or milliliter of said inoculant composition, optionally 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 , 1 x 10 13 , 1 x 10 14 , 1 x 10 15 or more colony forming units per gram and / or milliliter of said inoculant composition.
[00305] 4. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, essentially consisting of or consisting of spores of one or more diazotrophic microorganisms.
[00306] 5. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, consisting essentially of or consisting of spores of one or more strains of Bacillus, optionally, one or more strains of Bacillus circulans, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus polymyxa and / or Bacillus pumilus.
[00307] 6. The inoculant composition, according to any of the previous paragraphs, and said microbial cells / spores comprise, consist essentially of or consist of spores of one or more phosphate solubilizing microorganisms.
[00308] 7. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, consisting essentially of or consisting of spores of one or more strains of Penicillium, optionally one or more strains of P. bilaiae and / or P. gaestrivorus.
[00309] 8. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, essentially consisting of or consisting of spores of one or more strains of Trichoderma, optionally one or more strains of T. asperellum, T. atroviride, T. fertile, T. gamsii , T. hamatum, T. harzianum, T. reesi, T. virens and / or T. viridae.
[00310] 9. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, essentially consisting of or consisting of spores of one or more mycorrhizal fungi.
[00311] 10. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, consisting essentially of or consisting of spores of one or more strains of Gliocladium, optionally, one or more strains of Gliocladium virens, one or more strains of Glomus, optionally, one or more strains of Glomus intraradices, and / or one or more strains of Metarhizium, optionally one or more strains of Metarhizium anisopliae.
[00312] 11. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, consisting essentially of or consisting of spores of one or more strains of Bacillus, optionally B. amyloliquefaciens D747, B. amyloliquefaciens NRRL B-50349, B. amyloliquefaciens TJ1000, B. amyloliquefaciens FZB24, B. amyloliquefaciens FZB42, B. amyloliquefaciens IN937a, B. amyloliquefaciens IT-45, B. amyloliquefaciens TJ1000, B. amyloliquefaciens MBI600, B. amyloliquefaciens BS27 (deposited as NRRL B-5015), B. amyloliquefaciens deposited as 4 NRRL B-50013), B. amyloliquefaciens 15AP4 (deposited as ATCC PTA-6507), B. amyloliquefaciens 3AP4 (deposited as ATCC PTA-6506), B. amyloliquefaciens LSSA01 (deposited as NRRL B-50104), B. amyloliquefaciens ABP278 (deposited as NRRL B-50634), B. amyloliquefaciens 1013 (deposited as NRRL B-50509), B. amyloliquefaciens 918 (deposited as NRRL B-50508), B. amyloliquefaciens 22CP1 (deposited as ATCC PTA-6508), and B. amyloliquefaciens BS18 (deposited as NRRL B-50633), B. cereus 1-1562, B. firmus 1-1582, B. lichenformis BA842 (deposited as NRRL B-50516), B. lichenformis BL21 (deposited as NRRL B-50134) , B. mycoides NRRL B-21664, B. pumilus NRRL B-21662, B. pumilus NRRL B-30087, B. pumilus ATCC 55608, B. pumilus ATCC 55609, B. pumilus GB34, B. pumilus KFP9F, B. pumilus QST 2808, B. subtilis ATCC 55078, B. subtilis ATCC 55079, B. subtilis MBI 600, B. subtilis NRRL B-21661, B. subtilis NRRL B-21665, B. subtilis CX-9060, B. subtilis GB03, B subtilis GB07, B. subtilis QST-713, B. subtilis FZB24, B. subtilis D747, B. subtilis 3BP5 (deposited as NRRL B-50510), B. thuringiensis ATCC 13367, B. thuringiensis GC-91, B. thuringiensis NRRL B-21619, B. thuringiensis ABTS-1857, B. thuringiensis SAN 401 I, B. thuringiensis ABG-6305, B. thuringiensis ABG-6346, B. thuringiensis AM65-52, B. thuringiensis SA-12, B. thuringiensis SB4, B. thuringiensis ABTS-351, B. thuringiensis HD-1, B. thuringiensis EG 234 8, B. thuringiensis EG 7826, Bacil B. lus thuringiensis EG 7841, B. thuringiensis DSM 2803, B. thuringiensis NB-125 and / or B. thuringiensis NB-176.
[00313] 12. The inoculant composition, according to any of the previous paragraphs, and said microbial cells / spores comprise, consist essentially of or consist of spores of one or more strains of Gliocladium, optionally G. virens ATCC 52045 and / or G. virens GL-21, one or more strains of Glomus, optionally G. intraradices RTI-801, a or more strains of Metarhizium, optionally M. anisopliae F52, Penicillium, optionally P. 20851, P. bilaiae ATCC 22348, P. 50169, P. bilaiae NRRL 50776, P. 50778, P. bilaiae NRRL 50777, P. 50779, P. bilaiae NRRL 50780, P. 50782, P. bilaiae NRRL 50783, P. 50785, P. bilaiae NRRL 50786, P. 50788, P. bilaiae NRRL 67154, P. 67156, P. bilaiae NRRL 67157, P. bilaiae ATCC 18309, P. bilaiae ATCC bilaiae NRRL 50162, P. bilaiae NRRL bilaiae NRRL 50777, P. bilaiae NRRL bilaiae NRRL 50778, P. bilaiae NRRL bilaiae NRRL 50781, P. bilaiae NRRL bilaiae NRRL 5. 50787, P. bilaiae NRRL bilaiae NRRL 67155, P. bilaiae NRRL bilaiae NRRL 67158, P. bilaiae NRRL 67159, P. bilaiae RS7B-SD1, P. brevicompactum AgRF18, P. canescens ATCC 10419, P. expansum ATCC 24692, P. expansum YT02, P. fellatanum ATCC 48694, P. gaestrivorus NRRL 50170, P. glabrum DAOM 239074, P glabrum CBS 229.28, P. janthinellum ATCC 10455, P. lanosocoeruleum ATCC 48919, P. radicum ATCC 201836, P. radicum FRR 4717, P. radicum FRR 4719, P. radicum N93 / 47267 and / or P. raistrickii ATCC 10490, and / or a or more strains of Trichoderma, optionally T. asperellum SKT-1, T. asperellum ICC 012, T. atroviride LC52, T. atroviride CNCM 1-1237, T. fertile JM41R, T. gamsii ICC 080, T. hamatum ATCC 52198 , T. harzianum ATCC 52445, T. harzianum KRL-AG2, T. harzianum T-22, T. harzianum TH-35, T. harzianum T-39, T. harzianum ICC012, T. reesi ATCC 28217, T. virens ATCC 57678, T. virens Gl-3, T. virens GL-21, T. virens G-41, T. viridae ATCC 52440, T. viridae ICC080 and / or T. viridae TVL
[00314] 13. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, essentially consisting of or consisting of spores of one or more more strains that have a genomic sequence that is at least 75, 80, 85, 90, 95, 96, 97, 98, 99% or more identical to any of the strains recited in paragraph 11 based on 16S rDNA sequence identity .
[00315] 14. The inoculant composition, according to any of the preceding paragraphs, said microbial cells / spores comprising, consisting essentially of or consisting of spores of one or more strains having a genomic sequence that is at least 75, 80, 85, 90, 95, 96, 97, 98, 99% or more identical to any of the strains recited in paragraph 12 based on sequence identity of the internal transcribed spacer (ITS) and / or cytochrome c oxidase (CO1).
[00316] 15. The inoculant composition, according to any of the previous paragraphs, said microbial cells / spores comprising, essentially consisting of or consisting of spores of one or more biopesticides, optionally one or more biofungicides, one or more bioinsecticides and / or one or more bionematicides.
[00317] 16. The inoculant composition, according to any of the previous paragraphs, said carrier comprising about 50 to about 99% (by weight) of said inoculant composition, optionally about 75 to about 95% (by weight) of said composition, optionally about 70 , 70.5, 71, 71.5, 72, 72.5, 73, 73.5, 74, 74.5, 75, 75.5, 76, 76.5, 77, 77.5, 78, 78 ,5.79, 79.5, 80, 80.5, 81, 81.5, 82, 82.5, 83, 83.5, 84, 84.5, 85, 85.5, 86, 86.5 , 87, 87.5, 88, 88.5, 89, 89.5 or 90% (by weight) of said inoculant composition.
[00318] 17. The inoculant composition, according to any of the previous paragraphs, and said carrier comprises less than 0.01,0,02,0.03, 0.04,0,05,0,06,0.07, 0.08,0,09,0, 1.0.15, 0.2,0.25, 0.3,0.35, 0.4,0.45,0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%. 0.85%, 0.9%, 0.95% or 1% water by weight, based on the total weight of the composition.
[00319] 18. The inoculant composition of any of the previous paragraphs, in which said carrier does not comprise water.
[00320] 19. The inoculant composition of any of the preceding paragraphs, wherein said carrier comprises, essentially consists of or consists of a soil-compatible seed and / or carrier.
[00321] 20. The inoculant composition of any of the previous paragraphs, wherein said carrier additionally comprises one or more oils, optionally one or more mineral oils, nut oils and / or vegetable oils.
[00322] 21. The inoculant composition of any of the previous paragraphs, wherein said carrier additionally comprises one or more PEGs, optionally PEG 200, PEG 300 and / or PEG 400.
[00323] 22. The inoculant composition of any of the preceding paragraphs, wherein said carrier further comprises one or more PPGs, optionally PPG-9, PPG-10, PPG-17, PPG-20 and / or PPG-26.
[00324] 23. The inoculant composition of any of the previous paragraphs, further comprising one or more stabilizing compounds.
[00325] 24. The inoculant composition, in accordance with paragraph 23, said one or more stabilizing compounds comprising about 0.0001 to about 10% (by weight) of said composition, optionally, about 2 to about 6% (by weight) of said composition, optionally, about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0, 08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 1,1,5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 7.5, 8, 8.5, 9, 9.5 or 10% (by weight) of said composition.
[00326] 25. The inoculant composition of any of the paragraphs 23 or 24, said one or more stabilizing compounds comprising one or more hygroscopic polymers, optionally one or more albumins, alginates, celluloses, gums (e.g. cellulose gum, guar gum, acacia gum, combrite gum, xanthan gum), methylcellulose, nylons, pectins, polyacrylic acids, polycarbonates, PEGs, PEIs, polylactides, PMAs, polyurethanes, PVAs, PVPs, propylene glycols, sodium carboxymethylcelluloses and / or starches.
[00327] 26. The inoculant composition of any of the paragraphs 23 to 25, wherein said one or more stabilizing compounds comprise one or more oxidation control components, optionally one or more antioxidants (e.g. ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, a or more carotenoids, lipoic acid, one or more phenolic compounds (e.g. one or more flavonoids, flavones and / or flavonols), potassium ascorbate, sodium ascorbate, one or more thiols (e.g. glutathione, lipoic acid and / or or N-acetyl cysteine), one or more tocopherols, one or more tocotrienols, ubiquinone and / or uric acid), one or more oxygen scavengers, optionally ascorbic acid and / or sodium hydrogen carbonate, and / or one or more forming agents movie, optionally PVP / VA.
[00328] 27. The inoculant composition of any of the previous paragraphs, further comprising one or more dispersants, optionally, one or more of the dispersants expressly set out above.
[00329] 28. The inoculant composition, in accordance with paragraph 27, wherein said one or more dispersants comprise about 0.01 to about 5% (by weight) of said composition, optionally, about 0.1 to about 5% (by weight) of said composition, optionally , about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5 , 3, 3.5, 4, 4.5 or 5% (by weight) of said composition.
[00330] 29. The inoculant composition of any of the previous paragraphs, further comprising one or more pesticides, optionally, one or more of acaricides, fungicides, herbicides, insecticides and / or nematicides expressly set out above.
[00331] 30. The inoculant composition of any of the previous paragraphs, further comprising one or more LCOs, optionally, one or more LCOs represented by formulas I to IV.
[00332] 31. The inoculant composition of any of the paragraphs 1 to 29, further comprising one or more LCOs, optionally one or more of the LCOs represented by structures V to XXXIII.
[00333] 32. The inoculant composition of any of the paragraphs above, further comprising one or more chitin oligosaccharides, optionally, one or more chitin oligosaccharides represented by formulas XXXIV to XXXV.
[00334] 33. The inoculant composition of any of the paragraphs 1 to 31, further comprising one or more chitin oligosaccharides, optionally one or more chitin oligosaccharides represented by structures XXXVI to LXXXIII.
[00335] 34. The inoculant composition of any of the previous paragraphs, further comprising one or more chitinous compounds, optionally, one or more chitins and / or one or more chitosans.
[00336] 35. The inoculant composition of any of the previous paragraphs, additionally comprising one or more flavonoids, optionally, one or more of the anthocyanidins, anthoxanthines, flavanones, flavanonols, isoflavonoids, neoflavonoids and / or pterocarpans expressly set out above.
[00337] 36. The inoculant composition of any of the preceding paragraphs, further comprising jasmonic acid and / or one or more derivatives thereof.
[00338] 37. The inoculant composition of any of the previous paragraphs, further comprising linoleic acid and / or one or more derivatives thereof.
[00339] 38. The inoculant composition of any of the preceding paragraphs, further comprising linolenic acid and / or one or more derivatives thereof.
[00340] 39. The inoculant composition of any of the previous paragraphs, further comprising one or more carrachines, optionally, one or more carrachines represented by the formula LXXXIV.
[00341] 40. The inoculant composition of any of the previous paragraphs, additionally comprising one or more biostimulants, optionally, one or more seaweed extracts, one or more humic acids, one or more fulvic acids, myo-inositol and / or glycine.
[00342] 41. The inoculant composition of any of the previous paragraphs, further comprising one or more microbial extracts, optionally, one or more extracts of media comprising one or more diazotrophic microorganisms, phosphate solubilization and / or biopesticides.
[00343] 42. The inoculant composition of any of the preceding paragraphs, further comprising one or more nutrients, optionally one or more vitamins (e.g. vitamin A, vitamin B complex (i.e. vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7 , vitamin Bg, vitamin B9, vitamin B12, choline) vitamin C, vitamin D, vitamin E, vitamin K, carotenoids (a-carotene, p-carotene, cryptoxanthin, lutein, lycopene and / or zeaxanthin), macrominerals (e.g. calcium, iron, magnesium, nitrogen, phosphorus, potassium and / or sodium), trace minerals (e.g. boron, cobalt, chlorine, chromium, copper, fluorine, iodine, iron, manganese, molybdenum, selenium and / or zinc) and / or organic acids (e.g. acetic acid, citric acid, lactic acid, malic acid and / or taurine).
[00344] 43. The inoculant composition of any of the preceding paragraphs, further comprising one or more antifreeze agents, optionally ethylene glycol, glycerine, propylene glycol and / or urea.
[00345] 44. The inoculant composition, according to any of the previous paragraphs, said inoculant composition comprising a trace amount of water.
[00346] 45. The inoculant composition, according to any of the preceding paragraphs, with at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of said one or more microbial cells / spores remain viable when said inoculant composition is stored at 0, 1, 2, 3 , 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28 , 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 , 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00347] 46. The inoculant composition, according to any of the preceding paragraphs, with at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of said one or more microbial cells / spores remain viable when said inoculant composition is coated with plant propagation.
[00348] 47. The inoculant composition, according to any of the preceding paragraphs, with at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of said one or more microbial cells / spores remain viable when said inoculant composition is coated onto a plant propagation material and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 , 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00349] 48. The inoculant composition, according to any of the previous paragraphs, at least about 1 x 10 1 to about 1 x 10 10Colony forming units of said one or more microbial cells / spores per gram and / or milliliter of said inoculant composition remain viable when said inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8 , 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33 , 34, 35, 36, 37, 38, 39 and / or 40°C and 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85% or more relative humidity for a period of 1,2,3,4,5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more , optionally at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 or more colony forming units per gram and / or milliliter of said inoculant composition.
[00350] 49. The inoculant composition, according to any of the previous paragraphs, at least about 1 x 10 1 to about 1 x 10 10 Colony forming units of said one or more microbial cells / spores per seed remain viable when said inoculant composition is coated onto a seed and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of time from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more, optionally 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 or more colony forming units per seed.
[00351] 50. A coated plant propagation material that comprises, essentially consists of or consists of: a plant propagation material; and a coating covering at least a portion of an outer surface of said seed, said coating comprising, essentially consisting of or consisting of the inoculant composition according to any one of claims 1 to 49.
[00352] 51. The coated plant propagation material, in accordance with with paragraph 50, wherein said coating comprises, essentially consists of or consists of an inner coating layer comprising said microbial cells / spores and an outer coating layer which is devoid of said microbial cells / spores.
[00353] 52. Coated plant propagation material, of any one of paragraphs 50 or 51, said coating comprising about 1 x 10 1 to about 1 x 10 15 colony forming units of said microbial cells / spores, optionally 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 or more colony forming units.
[00354] 53. Coated plant propagation material, of any one of paragraphs 50 to 52, wherein said plant propagation material is a seed.
[00355] 54. Coated plant propagation material, in accordance with with paragraph 53, wherein said seed is a monocot.
[00356] 55. The coated plant propagation material, in accordance with with paragraph 53, wherein said seed is a dicot.
[00357] 56. The coated plant propagation material, in accordance with with paragraph 53, that said seed is a legume
[00358] 57. The coated plant propagation material, in accordance with with paragraph 53, that said seed is non-leguminous.
[00359] 58. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the Amaranthaceae family, optionally Swiss chard, spinach, sugar beet or quinoa.
[00360] 59. Coated plant propagation material, of any of paragraphs 50 to 53, wherein said plant propagation material is from the family Asteraceae, optionally artichoke, aster, chamomile, chicory, chrysanthemum, dahlias, daisies, echinacea, solidago, gaiule, lettuce, marigold, saffron, sunflowers or zinnias.
[00361] 60. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the Brassicaceae family, optionally arugula, broccoli, Chinese cabbage, Brussels sprouts, cabbage, cauliflower, canola, collard greens, white radish, garden cress, horseradish, kale, mustard, radish, rapeseed, turnip greens, turnip, wasabi, watercress or Arabidopsis thaliana.
[00362] 61. Coated plant propagation material, of any of paragraphs 50 to 53, wherein said plant propagation material is from the family Cucurbitaceae, optionally cantaloupe, cucumber, honeydew melon, melon, pumpkin, pumpkin (e.g. acorn squash, butternut squash, summer squash ), watermelon or zucchini.
[00363] 62. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the Fabaceae family, optionally alfalfa, beans, carob, clover, guar, lentils, mesquite, peas, peanuts, soybeans, tamarind, tragacanth or vicia.
[00364] 63. Coated plant propagation material, of any of paragraphs 50 to 53, where said material propagating plant is from the Malvaceae family, optionally cocoa, cotton, durium, hibiscus, kenaf, rye or okra.
[00365] 64. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the family Poaceae, optionally bamboo, barley, maize, fonio, grass (e.g. bay grass, bermuda grass, bluegrass, grass guinea fowl, centipede grass, fescue or Zoysia), millet, oats, ornamental grasses, rice, rye, sorghum, sugar cane, triticale or wheat.
[00366] 65. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the Polygonaceae family, optionally buckwheat.
[00367] 66. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is of the Rosaceae family, optionally almonds, apples, apricots, blackberries, blueberries, cherries, peaches, plums, quinces, raspberries, roses or strawberries.
[00368] 67. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the Solanaceae family, optionally bell peppers, chili peppers, eggplant, petunia, potatoes, tobacco or tomatoes.
[00369] 68. Coated plant propagation material, of any one of paragraphs 50 to 53, wherein said plant propagation material is from the Vitaceae family, optionally grape.
[00370] 69. A kit comprising: the propagation material of coated plant, from any one of paragraphs 50 to 68; and a container housing said coated plant propagation material.
[00371] 70. The kit, in accordance with paragraph 69, and said container reduces the amount of ambient light reaching said coated plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
[00372] 71. The kit, from any of paragraphs 69 or 70, being that said container reduces the amount of ambient oxygen reaching said plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
[00373] 72. The kit, from any of paragraphs 69 to 71, provided that said container comprises, consists essentially of or consists of a material having a light permeability of less than about 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35 , 40, 45, 50, 55, 60, 65, 70 or 75%.
[00374] 73. The kit, from any of paragraphs 69 to 72, provided that said container comprises, consists essentially of or consists of a material having an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 cm 3 / m 2 -day (as measured in accordance with ASTM D3985).
[00375] 74. The kit, from any of paragraphs 69 to 73, in which the said kit further comprises one or more of an oxygen absorbing compound, optionally activated carbon, iron powder, sodium chloride, iron carbonate, one or more metal halide catalysts and / or sodium hydrogen carbonate.
[00376] 75. A plant treated with the inoculant composition, of any of paragraphs 1 to 49.
[00377] 76. A plant germinated from the material of coated plant propagation, from any of paragraphs 50 to 68.
[00378] 77. A plant part harvested from the plant, from either of paragraphs 75 or 76.
[00379] 78. A processed product produced from the part of plant, in accordance with paragraph 77.
[00380] 79. A culture that understands, essentially consists of or consists of a plurality of the plant or plant part of any one of paragraphs 75 to 77.
[00381] 80. A method that comprises, essentially consists of or consists of applying the inoculant composition of any one of paragraphs 1 to 49 to a plant propagation material.
[00382] 81. The method, in accordance with paragraph 80, provided that further comprises planting said plant propagation material in a growing medium, optionally, soil.
[00383] 82. The method, in accordance with paragraph 81, in which said plant propagation material is planted in soil in which plants of the same genus were grown in at least one of the three years prior to said planting, optionally in each of the one, two or three years immediately preceding said planting.
[00384] 83. The method, in any of paragraphs 80 to 82, in that said inoculant composition is applied to the plant propagation material at the time of planting.
[00385] 84. The method, of any one of paragraphs 80 to 82, being that said inoculant composition is applied to plant propagation material at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 18, 21, 24, 27, 30 , 33, 36, 39, 42, 45, 48 hours or more before planting.
[00386] 85. The method, of any one of paragraphs 80 to 82, being that said inoculant composition is applied to plant propagation material at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18 , 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more before planting.
[00387] 86. The method, of any one of paragraphs 80 to 82, being that said inoculant composition is applied to the plant propagation material about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18 , 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36 months or more of planting
[00388] 87. The method, in any of paragraphs 80 to 86, in that said plant propagation material is a seed.
[00389] 88. The method, in any of paragraphs 80 to 87, in that said plant propagation material is a monocot.
[00390] 89. The method, in any of paragraphs 80 to 87, in that said plant propagation material is a dicot.
[00391] 90. The method, in any of paragraphs 80 to 87, in that said plant propagation material is a legume.
[00392] 91. The method, in any of paragraphs 80 to 87, in that said plant propagation material is non-leguminous.
[00393] 92. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Amaranthaceae family, optionally chard, spinach, sugar beet or quinoa.
[00394] 93. The method, of any one of paragraphs 80 to 87, in that said plant propagation material is of the Asteraceae family, optionally artichoke, aster, chamomile, chicory, chrysanthemum, dahlias, daisies, echinacea, solidago, gaiule, lettuce, marigolds, saffron, sunflowers or zinnias.
[00395] 94. The method, in any of paragraphs 80 to 87, in that said plant propagation material is of the Brassicaceae family, optionally arugula, broccoli, Chinese cabbage, Brussels sprouts, cabbage, cauliflower, canola, collard greens, white radish, garden cress, horseradish, kale, mustard, radish, rapeseed, turnip greens, turnip, wasabi, watercress or Arabidopsis thaliana.
[00396] 95. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the family Cucurbitaceae, optionally cantaloupe, cucumber, honeydew melon, melon, pumpkin, squash (e.g. acorn squash, butternut squash, summer squash), watermelon or zucchini.
[00397] 96. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Fabaceae family, optionally alfalfa, beans, carob, clover, guar, lentils, mesquite, peas, peanuts, soybeans, tamarind, tragacanth or vicia.
[00398] 97. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Malvaceae family, optionally cocoa, cotton, durium, hibiscus, kenaf, rye or okra.
[00399] 98. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Poaceae family, optionally bamboo, barley, maize, fonio, grass (e.g. bay grass, bermuda grass, blue grass, guinea grass, centipede grass, fescue or Zoysia), millet, oats, ornamental grasses, rice, rye, sorghum, sugar cane, triticale or wheat.
[00400] 99. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Polygonaceae family, optionally buckwheat.
[00401] 100. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Rosaceae family, optionally almonds, apples, apricots, blackberries, blueberries, cherries, peaches, plums, quinces, raspberries, roses or strawberries.
[00402] 101. The method, in any of paragraphs 80 to 87, in that said plant propagation material is from the Solanaceae family, optionally peppers, chilli peppers, eggplant, petunia, potatoes, tobacco or tomatoes.
[00403] 102. The method, in any of paragraphs 80 to 87, in that the seed plant propagation material is from the Vitaceae family, optionally grape.
[00404] 103. A method that comprises, essentially consists of or consists of planting the coated plant propagation material of any one of paragraphs 50 to 68 in a growing medium, optionally soil.
[00405] 104. The method, in any of paragraphs 80 to 103, which further comprises applying the inoculant composition of any one of paragraphs 1 to 49 to the plant growing from the plant propagation material.
[00406] 105. A method to improve stability and / or ability of one or more microorganisms to survive in a composition comprising one or more paraffin oils and / or waxes, said method comprising, essentially consisting of or consisting of: adding one or more stabilizing compounds to said composition.
[00407] 106. The method, in accordance with paragraph 105, in which said one or more stabilizing compounds comprises, consists essentially of or consists of:
[00408] one or more hygroscopic polymers, optionally one or more albumins, alginates, celluloses, gums (for example, cellulose gum, guar gum, acacia gum, combrete gum, xanthan gum), methylcellulose, nylons, pectins, polyacrylic acids polycarbonates, PEGs, PEIs, polylactides, PMAs, polyurethanes, PVAs, PVPs, propylene glycols, sodium carboxymethylcelluloses and / or starches; and / or
[00409] Oxidation control components, optionally, one or more antioxidants (for example, ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, one or more carotenoids, lipoic acid, one or more phenolic compounds (for example , one or more flavonoids, flavones and / or flavonols), potassium ascorbate, sodium ascorbate, one or more thiols (e.g. glutathione, lipoic acid and / or N-acetyl cysteine), one or more tocopherols, one or more tocotrienols, ubiquinone and / or uric acid), one or more oxygen scavengers, optionally acid ascorbic acid and / or sodium hydrogen carbonate and / or one or more film forming agents, optionally PVP / VA.
[00410] 107. The method, of any of paragraphs 105-106, said one or more stabilizing compounds being added until it comprises about 0.0001 to about 10% (by weight) of said composition, optionally about 2 to about 6% (by weight) of said composition, optionally about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08,0.09,0,1,0.15, 0.2,0.25, 0.3,0.35, 0.4, 0.45, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 7.5, 8, 8.5, 9, 9.5 or 10% (by weight) of said composition.
[00411] 108. The method, in any of paragraphs 105 to 107, said one or more stabilizing compounds being added in an amount sufficient to ensure that at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of said microbial cells / spores remain viable when said inoculant composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 , 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00412] 109. The method, in any of paragraphs 105 to 108, said one or more stabilizing compounds being added in an amount sufficient to ensure that at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of said microbial cells / spores remain viable when said inoculant composition is coated in a plant propagation material.
[00413] 110. The method, in any of paragraphs 105 to 109, said one or more stabilizing compounds being added in an amount sufficient to ensure that at least about 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more of said microbial cells / spores remain viable when said inoculant composition is coated in a plant propagation material and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 , 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 25, 30 , 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
[00414] 111. The method, in any of paragraphs 105 to 110, said one or more stabilizing compounds being added in an amount sufficient to ensure that at least about 1 x 10 1 to about 1 x 10 15colony forming units of said microbial cells / spores per gram and / or milliliter of said inoculating composition remain viable when said inoculating composition is stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and / or 40°C and 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32 , 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more, optionally at least 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x 10 10 , 1 x 10 11 , 1 x 10 12 or more colony forming units per gram and / or milliliter of said inoculant composition.
[00415] 112. The method, in any of paragraphs 105 to 111, said one or more stabilizing compounds being added in an amount sufficient to ensure that at least about 1 x 10 1 about from 1 x 10 15 Colony forming units of said microbial cells / spores per seed remain viable when said inoculant composition is coated onto a seed and stored at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36 , 37, 38, 39 and / or 40°C and 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more optionally 1 x 10 1 , 1 x 10 2 , 1 x 10 3 , 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 ,1 x 10 10 ,1 x 10 11 ,1 x 10 12 or more colony forming units per seed.
[00416] 113. The method of any of paragraphs 105 to 112, being further comprising adding one or more dispersants to said composition.
[00417] 114. The method, in accordance with paragraph 113, in which said one or more dispersants comprising one or more anionic surfactants, optionally one or more of the anionic surfactants expressly set forth above; one or more cationic surfactants, optionally one or more of the cationic surfactants expressly set out above; one or more nonionic surfactants, optionally one or more of the nonionic surfactants expressly set forth above; and / or one or more wetting agents, optionally one or more of the wetting agents set out above.
[00418] 115. The inoculant composition of any of the paragraphs 113 to 114, wherein said one or more dispersants comprise one or more polyoxyethylene alkyl ethers, one or more acrylic copolymers, one or more polyoxyethylene sorbitan trioleates and / or one or more secondary alcohol ethoxylates.
[00419] 116. The method, in any of paragraphs 105 to 115, which also includes adding one or more microbial extracts not aqueous to said composition.
[00420] 117. The method, in accordance with paragraph 116, considering that the said one or more non-aqueous microbial extracts comprise: one or more Bacillus extracts, optionally, an extract of media comprising B. amyloliquefaciens D747, B. amyloliquefaciens NRRL B-50349, B. amyloliquefaciens TJ1000, B. amyloliquefaciens FZB24, B. amyloliquefaciens FZB42, B. amyloliquefaciens IN937a, B. amyloliquefaciens IT-45, B. amyloliquefaciens TJ1000, B. amyloliquefaciens MBI600, B. amyloliquefaciens BS27 (deposited as NRRL B-5015), B. amyloliquefaciens BS2084 (deposited as NRRL B-50013), B. amyloliquefaciens 15AP4 (deposited as ATCC PTA-6507), B. amyloliquefaciens 3AP4 (deposited as ATCC PTA-6506), B. amyloliquefaciens LSSA01 (deposited as NRRL B-50104), B. amyloliquefaciens ABP278 (deposited as NRRL B-50634), B. amyloliquefaciens 1013 (deposited as NRRL B-50509), B. amyloliquefaciens 918 (deposited as NRRL B-50508), B. amyloliquefaciens 22CP1 (deposited as ATCC PTA-6508), and B. amyloliquefaciens BS 18 (deposited as NRRL B-50633), B. cereus 1-1562, B. firmus 1- 1582, B. lichenformis BA842 (deposited as NRRL B-50516), B. lichenformis BL21 (deposited as NRRL B-50134), B. mycoides NRRL B-21664, B. pumilus NRRL B-21662, B. pumilus NRRL B- 30087, B. pumilus ATCC 55608, B. pumilus ATCC 55609, B. pumilus GB34, B. pumilus KFP9F, B. pumilus QST 2808, B. subtilis ATCC 55078, B. subtilis ATCC 55079, B. subtilis MBI 600, B. subtilis NRRL B-21661, B. subtilis NRRL B-21665, B. subtilis CX-9060, B. subtilis GB03, B. subtilis GB07, B. subtilis QST-713, B. subtilis FZB24, B. subtilis D747, B. subtilis 3BP5 (deposited as NRRL B-50510), B. thuringiensis ATCC 13367 , B. thuringiensis GC-91, B. thuringiensis NRRL B-21619, B. thuringiensis ABTS-1857, B. thuringiensis SAN 401 I, B. thuringiensis ABG-6305, B. thuringiensis ABG-6346, B. thuringiensis AM65-52, B. thuringiensis SA-12, B. thuringiensis SB4, B. thuringiensis ABTS-351, B. thuringiensis HD-1, B. thuringiensis EG 2348, B. thuringiensis EG 7826, B. thuringiensis EG 7841, B thuringiensis DSM 2803, B. thuringiensis NB-125 and / or B. thuringiensis NB-176; one or more extracts of Bradyrhizobium, optionally one extract from media comprising B. elkanii SEMI A 501, B. elkanii SEMI A 587, B. elkanii SEMIA 5019, B. japonicum NRRL B-50586 (also deposited as NRRL B-59565), B. japonicum NRRL B-50587 (also deposited as NRRL B-59566), B. japonicum NRRL B-50588 ( also deposited as NRRL B-59567), B. japonicum NRRL B-50589 (also deposited as NRRL B-59568), B. japonicum NRRL B-50590 (also deposited as NRRL B-59569), B. japonicum NRRL B-50591 (also deposited as NRRL B-59570), B. japonicum NRRL B-50592 (also deposited as NRRL B-59571), B. japonicum NRRL B-50593 (also deposited as NRRL B-59572), B. japonicum NRRL B- 50594 (also deposited as NRRL B-50493), B. japonicum NRRL B-50608, B. japonicum NRRL B-50609, B. japonicum NRRL B-50610, B. japonicum NRRL B-50611, B. japonicum NRRL B-50612, B. japonicum NRRL B-50726, B. japonicum NRRL B-50727, B. japonicum NRRL B-50728, B. japonicum NRRL B-50729, B. japonicum NRRL B-50730, B. japonicum SEMIA 566, B. japonicum SEMIA 5079, B. japonicum SEMIA 5080, B. japonicum USDA 6, B. japonicum USDA 110, B. japonicum USDA 122, B. japonicum USDA 123, B. japonicum USDA 127, B. japonicum USDA 129 and / or B. japonicum USDA 532C; one or more extracts of Rhizobium, optionally an extract of media comprising R. leguminosarum SO12A-2; one or more Sinorhizobium extracts, optionally an extract from media comprising S.fredii CCBAUl 14 and / or S.fredii USDA 205; one or more extracts of Penicillium, optionally, an extract of media comprising P. bilaiae ATCC 18309, P. bilaiae ATCC 20851, P. bilaiae ATCC 22348, P. bilaiae NRRL 50162, P. bilaiae NRRL 50169, P. bilaiae NRRL 50776, bilaiae NRRL 50777, bilaiae NRRL 50780, bilaiae NRRL 50783, bilaiae NRRL 50786, P. bilaiae NRRL 50777, P. P. bilaiae NRRL 50778, P. P. bilaiae NRRL 50781, P. P. bilaiae NRRL 50784, P. P. bilaiae NRRL 50787, P. bilaiae NRRL 50778, P. bilaiae NRRL 50779, P. bilaiae NRRL 50782, P. bilaiae NRRL 50785, P. bilaiae NRRL 50788, P. bilaiae RS7B-SD1, P. brevicompactiim AgRF18, P. canescens ATCC 10419, P. expansion ATCC 24692, P. expansum YT02, P. fellatanum ATCC 48694, P. gaestrivorus NRRL 50170, P. glabrum DAOM 239074, P. glabrum CBS 229 ,28, P. janthinellum ATCC 10455, P. lanosocoeruleum ATCC 48919, P. radicam ATCC 201836, P. radicion FRR 4717, P. radicion FRR 4719, P. radicam N93 / 47267 and / or P. raistrickii ATCC 10490; one or more extracts of Streptomyces, optionally, an extract of media comprising Streptomyces NRRL B-30145, Streptomyces M1064, S. galbus NRRL 30232, S. lydicus WYEC 108 (ATCC 55445), S. violaceusniger YCED 9 (ATCC 55660), and / or Streptomyces WYE 53 (ATCC 55750); and / or one or more extracts of Trichoderma, optionally, an extract of media comprising T. asperellum SKT-1 (ECO-HOPE®, Kumiai Chemical Industry Co., Ltd., Japan), T. atroviride LC52 (SENTINEL®, Agrimm Technologies Ltd , NZ), T. harzianum T-22 (PLANTSHIELD®, der Firma BioWorks Inc., USA), T. harzianum TH-35 (ROOT PRO®, with Mycontrol Ltd., Israel), T. harzianum T-39 ( TRICHODEX®, Mycontrol Ltd., Israel; T. 2000®, Makhteshim Ltd., Israel), T. harzianum ICC012 and T. viride TRICHOPEL (Agrimm Technologies Ltd, NZ), T. harzianum ICC012 and T. viride ICC080 (REMEDIER® WP, Isagro Ricerca, Italy), T. polysporum and T. harzianum (BINAB®, BINAB Bio- Innovation AB, Sweden), T. stromaticum TRICOVAB® (C.E.P.L.A.C., Brazil), T. virens GL-21 (SOILGARD®, Certis LLC, USA), T. virens Gl-3, ATCC 57678, T. virens Gl-21 ( Thermo Trilogy Corporation, Wasco, CA), T. virens Gl-3 and Bacillus amyloliquefaciens FZB2, T. virens Gl-3 and Bacillus amyloliquefaciens NRRL B-50349, T. virens Gl-3 and Bacillus amyloliquefaciens TJ1000, T. virens Gl- 21 and Bacillus amyloliquefaciens FZB24, T. virens Gl-21 and Bacillus amyloliquefaciens NRRL B-50349, T. virens Gl-21 and Bacillus amyloliquefaciens TJ1000, T. viride TRIECO® (Ecosense Labs. (India) Pvt. Ltd., India, BIO-CURE® F from T. Stanes & Co. Ltd., India), T. viride TV1 (Agribiotec srl, Italy), T. viride ICC080.
[00421] 118. The inoculant composition of any of the paragraphs 116 or 117, said one or more non-aqueous microbial extracts comprising less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5% water by weight based on weight total composition.
[00422] 119. The method, of any of paragraphs 116 to 118, said one or more non-aqueous microbial extracts being added until it comprises about 0.1 to about 5% (by weight) of said composition, optionally about 0.1 to about 2% (by weight) of said composition, optionally about 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0, 6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1, 5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2, 8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4., 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of said composition.
[00423] 120. A method that comprises, essentially consists of or consists of applying the inoculant composition of any one of paragraphs 1 to 49 and a second composition to a seed and / or to the plant growing from said seed, said second composition comprising: one or more agriculturally beneficial microorganisms, optionally one or more diazotrophs, one or more phosphate solubilizing microorganisms, one or more mycorrhizal fungi and / or one or more biopesticides, optionally one or more biofungicides, one or more bioinsecticides and / or one or more bionematicides; one or more biostimulants, optionally one or more kelp extracts, one or more humic acids, one or more fulvic acids, myo-inositol and / or glycine; one or more nutrients, optionally one or more vitamins (e.g. vitamin A, vitamin B complex (i.e. vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B8, vitamin B9, vitamin B12, choline) vitamin C, vitamin D, vitamin E, vitamin K, carotenoids (α-carotene, β-carotene, cryptoxanthin, lutein, lycopene, and / or zeaxanthin), macrominerals (eg, calcium, iron, magnesium, nitrogen , phosphorus, potassium and / or sodium), trace minerals (e.g. porpoise, cobalt, chlorine, chromium, copper, fluoride, iodine, iron, manganese, molybdenum, selenium and / or zinc) and / or organic acids (e.g. , acetic acid, citric acid, lactic acid, malic acid and / or taurine); one or more acaricides, optionally one or more of the acaricides expressly set out above; one or more fungicides, optionally one or more of the fungicides expressly set out above; one or more herbicides, optionally one or more of the herbicides expressly set out above; one or more insecticides, optionally one or more of the insecticides expressly set forth above; one or more nematicides, optionally one or more of the insecticides expressly set out above; one or more LCOs, optionally one or more of t...
Claims
CLAIMS 1. Use of one or more paraffin oils and / or waxes, characterized by the fact that it is used to stabilize bacterial cells and / or spores.
2. Liquid inoculant composition, characterized in that it comprises bacterial cells and / or spores and one or more paraffin oils and / or waxes, wherein said one or more paraffin oils and / or waxes are present in an amount effective to enhance the survival of said bacterial cells and / or spores.
3. Inoculant composition according to claim 2, characterized in that said bacterial cells and / or spores comprise from about 5 to about 20% (by weight) of said inoculant composition, optionally about 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5 or 20% (by weight) of said inoculant composition.
4. Inoculant composition according to claim 2 or 3, characterized by the fact that said bacterial cells and / or spores comprise approximately 1 x 10 4 approximately 1 x 10 15 colony-forming units per gram and / or milliliter of said inoculant composition, optionally at least 1 x 10 4 , 1 x 10 5 , 1 x 10 6 , 1 x 10 7 , 1 x 10 8 , 1 x 10 9 , 1 x IO 10 , 1 x 10 11 , 1 x 10 12 colony-forming units per gram and / or milliliter of said inoculant composition.
5. Inoculant composition according to any of the claims 2 to 4, characterized in that said one or more paraffin oils and / or waxes comprise about 75 to about 95% (by weight) of said inoculating composition, optionally about 75, 75.5, 76, 76.5, 77, 77.5, 78, 78.5, 79, 79.5, 80, 80.5, 81, 81.5, 82, 82.5, 83, 83.5, 84, 84.5, 85 85.5, 86, 86.5, 87, 87.5, 88, 88.5, 89, 89.5, 90, 90.5, 91, 91.5, 92, 92.5, 93, 93.5, 94, 94.5 or 95% (by weight) of said inoculating composition.
6. Inoculant composition according to any one of claims 2 to 5, wherein said inoculant composition is characterized in that it additionally comprises one or more dispersants.
7. Inoculant composition according to claim 6, characterized in that said one or more dispersants comprise from about 0.1 to about 5% (by weight) of said composition, optionally about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5% (by weight) of said composition.
8. Inoculant composition according to any one of claims 2 to 7, characterized in that at least 50% of said bacterial cells and / or spores exist as single isolated cells and / or spores when said inoculant composition is diluted 1:10 in water.
9. Inoculant composition according to any one of claims 2 to 8, characterized in that at least 50% of said bacterial cells and / or spores remain viable when said inoculant composition is stored at 20 to 25 °C and 35 to 65% relative humidity for a period of 12 weeks.
10. Inoculant composition according to any one of claims 2 to 9, characterized in that at least 50% of said bacterial cells and / or spores remain viable when said inoculant composition is coated onto a seed and then stored at 20 to 25 °C and 35 to 65% relative humidity for a period of 12 weeks.
11. Inoculating composition according to any one of claims 2 to 10, wherein said inoculating composition is characterized in that it comprises no more than 0.5% water (by weight).
12. Coated plant seed, characterized in that it comprises a plant seed and a coating covering at least a portion of an external surface of said seed, said coating comprising the inoculating composition, as defined in any of claims 2 through 11.
13. Kit, characterized in that it comprises coated plant propagation material as defined in claim 12, and a container housing said coated plant propagation material.
14. Method, characterized in that it comprises applying the inoculation composition as defined in any one of claims 2 to 11, to a plant propagation material, optionally a seed, and / or to a plant growing from said plant propagation material.
15. Method, characterized in that it comprises introducing the inoculation composition as defined in any one of claims 2 to 11 into a plant culture medium, optionally a soil. CFU / semeirte weeks FIGURE 1 20-il ? - 0S 8 oa#® 1 ' 05 ' SUMMARY USE OF ONE OR MORE OILS AND / OR PARAFFIN WAXES, LIQUID INOCULANT COMPOSITION, COATED PLANT SEED, KIT, AND METHOD The present disclosure provides inoculant compositions and methods for enhancing the survival and / or stability of microbial cells and / or spores in an inoculant composition. In some embodiments, inoculant compositions of the present disclosure comprise microbial cells and / or spores in a carrier comprising paraffin oils and / or waxes.