Use of filamentous fungi belonging to the genus Talaromyces and a method for controlling a plant disease.

Abstract

Plant disease control agents and methods for controlling plant diseases. The present invention addresses the problem of providing an agent to control diseases induced in a plant by a bacterium selected from the genus Rhizoctonia, genus Fusarium, genus Pythium, genus Microdochium, genus Tilletia, genus Ustilago, genus Streptomyces, genus Erwinia and genus Aphanomyces, the plants selected from cruciferous plants, beans, corn, wheat, potatoes and sugar beets. In the present invention, this disease can be controlled by treating seeds, seedlings or soil of these plants using a fungus belonging to the genus Talaromyces.

Description

"USE OF FILAMENTOUS FUNGI BELONGING TO THE GENUS TALAROMYCES AND A METHOD FOR CONTROLLING A PLANT DISEASE" FIELD OF THE INVENTION

[0001] The present invention relates to a control agent for controlling a disease that infects plants, such as grains and vegetables eaten by humans and animals, and a method of controlling diseases using the same. BACKGROUND OF THE INVENTION

[0002] With the rapid increase in the world's population, the demand for food is rising, and more efficient and highly productive farming is needed in a limited growing area. On the other hand, crop yields are declining due to climate change caused by global warming and similar factors.

[0003] Currently, agricultural diseases are mainly controlled by the use of chemical pesticides, but due to their overuse, pathogens resistant to these drugs have emerged, and environmental pollution from the pesticides themselves has become a problem. On the other hand, a method of controlling crop diseases that does not rely on chemical pesticides and has a low environmental impact is an important means for the sustainable development of agriculture.

[0004] In recent years, microbial pesticides have been developed that use microorganisms to control plant diseases. As an example of such a microorganism, a filamentous fungus belonging to the genus Talaromyces is known. For example, Patent Document 1 (JP 3601928 B) discloses a strain of Talaromyces flavus Y-9401 having a control effect on strawberry anthracnose.

[0005] Furthermore, Patent Documents 2 to 4 show that Talaromyces flavus has a control effect on tomato Botrytis cinerea and powdery mildew, and Patent Documents 5 to 7 show that a control effect on rice diseases has been observed.

[0006] Furthermore, Non-Patent Document 1 describes that when a solid culture of the fungus Talaromyces flavus was sprayed onto contaminated soil Petition 870240016976, dated 29 / 02 / 2024, page 15 / 80 2 / 28 prepared by spraying Sclerotinia sclerotirum sclerotia, the occurrence of sclerotia rot can be controlled in soybeans, rapeseed, wheat and barley.

[0007] In Non-Patent Document 2, it is shown that potatoes sterilized by immersion in a suspension of Talaromyces flavus spores suppressed the occurrence of mid-body wilt disease (causative bacterium Verticillium albo-atrum). Furthermore, the same document shows that mid-body wilt disease can be controlled even when non-sterilized potatoes are sown directly in soil treated with Tararomyces flavus spores.

[0008] Non-Patent Document 3 shows that stem rot (causative bacterium Sclerotinia sclerotirum) can be controlled by spraying a suspension of Talaromyces flavus on potato seedlings.

[0009] Non-Patent Document 4 shows that midbody wilt disease (causative bacterium Verticillium dahliae) can be controlled by treating eggplant and potato seeds or tubers with a simple preparation containing Talaromyces flavus spores.

[0010] Non-Patent Document 5 shows that the seedling blight caused by Rhizoctonia solani can be controlled by treating beet seeds with Talaromyces flavus. STATE OF THE ART DOCUMENTS Patent Documents

[0011] [Patent Document 1] JP 3601928 B

[0012] [Patent Document 2] JP 4226323 B

[0013] [Patent Document 3] JP 2006-124337 A

[0014] [Patent Document 4] JP 2009-221132 A

[0015] [Patent Document 5] JP 4810151 B

[0016] [Patent Document 6] JP ​​​​2014-237609 A

[0017] [Patent Document 7] JP 2015-093850 A Non-Patent Documents

[0018] [Non-Patent Document 1] MCLAREN DL, HUANG HC, RIMMERS R. (1996), Control of Apothecial Production of Sclerotinia sclerotiorum by Coniothyrium Petition 870240016976, dated 29 / 02 / 2024, p. 16 / 80 3 / 28 minitans and Talaromyces flavus. Plant Dis Vol. 80 No. 12 Page. 1373-1378

[0019] [Documento Não Patentário 2] Naraghi, L., Heydari, A., Rezaee, S., Razavi, M., & Jahanifar, H. (2010). Study on antagonistic effects of Talaromyces flavus on Verticillium albo-atrum, the causal agent of potato wilt disease. Crop Protection, 29(7), 658-662.

[0020] [Documento Não Patentário 3] OJAGHIAN Mohammad Reza (2011) Potential of Trichoderma spp. and Talaromyces flavus for biological control of potato stem rot caused by Sclerotinia sclerotiorum. Phytoparasitica Vol. 39 No. 2 Page. 185193

[0021] [Documento Não Patentário 4] Nagtzaam, M. P. M., & Bollen, G. J. (1997). Colonization of roots of eggplant and potato by Talaromyces flavus from coated seed. Soil Biology and Biochemistry, 29(9-10), 1499-1507.

[0022] [Non-Patent Document 5] Kakvan, N., Heydari, A., Zamanizadeh, H.R., Rezaee, S., & Naraghi, L. (2013). Development of new bioformulations using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damp-off disease. Crop Protection, 53, 80-84. SUMMARY OF THE INVENTION PROBLEMS TO BE SOLVED BY THE INVENTION

[0023] As described above, filamentous fungi belonging to the genus Talaromyces are known to be useful as disease control agents. However, their application to a wide range of plant types and diseases has been in demand.

[0024] An object of the present invention is to provide a microbial preparation for a plant selected from the group consisting of Brassicaceae plants; beans; maize; wheat varieties; tubers and roots; and sugar beet; which microbial preparation is used for the control of a disease caused by a fungus selected from the group consisting of fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to Petition 870240016976, dated 29 / 02 / 2024, page 17 / 80 4 / 28 genus Streptomyces, fungi belonging to the genus Erwinia and fungi belonging to the genus Aphanomyces. WAYS TO SOLVE THE PROBLEMS

[0025] In order to solve the above problem, the present inventors have intensively studied and determined that, by treating seeds, seedlings, soil and / or the like of a selected plant from the group consisting of Brassicaceae plants; beans; corn; wheat variety; tubers and roots; and sugar beet; with a filamentous fungus belonging to the genus Talaromyces, stable and efficient control of diseases caused by fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia and fungi belonging to the genus Aphanomyces can be achieved, and thus completed the present invention.

[0026] The essence of the present invention is as follows.

[0027] [1] A disease control agent for a plant selected from the group consisting of Brassicaceae plants; beans; maize; wheat variety; tubers and roots; and sugar beet; the control agent comprising, as an active ingredient, a filamentous fungus belonging to the genus Talaromyces,

[0028] wherein the said disease is caused by a fungus selected from the group consisting of: fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia and fungi belonging to the genus Aphanomyces.

[0029] [2] The control agent according to [1], wherein the filamentous fungus belonging to the genus Talaromyces is Talaromyces flavus.

[0030] [3] The control agent according to [2], in which Talaromyces flavus Petition 870240016976, dated 29 / 02 / 2024, page 18 / 80 5 / 28 is strain Talaromyces flavus Y-9401 (FERM BP-10642).

[0031] [4] The control agent, according to any one of [1] to [3], wherein said agent comprises the filamentous fungus belonging to the genus Talaromyces in an amount of 1 χ 106a 1 χ 1012 colony forming units (cfu) / g.

[0032] [5] The control agent, according to any one of [1] to [4], in which the Brassicaceae plants are selected from the group consisting of broccoli, cabbage, cauliflower, kale, turnip, Chinese cabbage (hakusai), Japanese radish (daikon), Japanese horseradish (wasabi) and watercress.

[0033] [6] The control agent, according to any one of [1] to [5], further comprising a surfactant and / or an excipient.

[0034] [7] The control agent, according to [6], wherein the surfactant is one or more surfactants selected from the group consisting of fatty acid soaps, alkyl ether carboxylic acid, N-acylamino acid, alkyl benzene sulfonate, alkyl naphthalene sulfonate, alpha-sulfonate olefin, dialkyl sulfosuccinate salt, higher alcohol sulfate salt, alkyl ether sulfate, polyoxyethylene alkylphenyl ether sulfate, fatty acid amide alkylol sulfate salt, alkyl ether phosphate salt, alkyl phosphate salt, aliphatic amine salt, aliphatic quaternary ammonium salt, benzalkonium salt, benzethonium chloride, pyridinium salt, imidazolinium salt, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene polyoxypropylene block polymer, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene glycerin fatty acid ester, polyoxyethylene castor oil, polyoxyethylene sorbitol fatty acid ester, polyethylene sorbitolpolyoxyethylene sorbitol fatty acid ester, fatty acid monoglyceride, glycerin fatty acid ester, sorbitan fatty acid ester, fatty acid alkanolamide, polyoxyethylene fatty acid amide, polyoxyethylene alkylamine, alkylamine oxide, carboxybetaine, imidazolinium aminocarboxylate and betaine.

[0035] [8] The control agent, according to [6] or [7], wherein the excipient is one or more excipients selected from the group consisting of: Selected fine mineral powders from the group consisting of kaolin clay, Petition 870240016976, dated 29 / 02 / 2024, p. 19 / 80 6 / 28 pyrophyllite clay, bentonite, montmorillonite, diatomaceous earth, synthetic hydrated silicon oxide, acid clay, talc, clay, ceramics, quartz, sericite, vermiculite, perlite, Oya stone, anthracite, limestone, coal and zeolite; Selected inorganic compounds from the group consisting of common salt, carbonates, sulfates, nitrates and urea; Selected organic matter from the group consisting of tares, bran, crab shells, shrimp shells, krill powder, rice husks, wheat flour, corn cobs, peanut shells, bone meal, fish meal, powdered husks, wood flour, charcoal, kuntan charcoal, tree bark charcoal, rice husk charcoal, vegetable / wood charcoal, peat, attapulgite, dried manure, activated charcoal, oil and starch cake and their hydrolysates; Selected saccharides from the group consisting of D-sorbitol, lactose, maltitose, glucosamine and oligosaccharides; Selected oils from the group consisting of vegetable oils, animal oils and mineral oils; and water-soluble synthetic polymers.

[0036] [9] The control agent, according to any one of [6] to [8], said control agent comprising the filamentous fungus belonging to the genus Talaromyces in 1 to 90% by mass, the surfactant in 1 to 15% by mass, and the excipient in 0 to 98% by mass.

[0037]

[10] The control agent, according to any one of [6] to [8], said control agent comprising the fungus belonging to the genus Talaromyces in 2 to 46% by mass, the surfactant in 2 to 15% by mass, and the excipient in 39 to 96% by mass.

[0038]

[11] The control agent, according to any one of [1] to

[10] , wherein said control agent is formulated as any one of a powder, a granule, an emulsion, a wettable powder, a fluid formulation and a coating agent.

[0039]

[12] A method of controlling a plant disease, the method comprising the step of: treating seeds, seedlings, plant bodies, soil Petition 870240016976, dated 29 / 02 / 2024, page 20 / 80 7 / 28 of seedling culture, seedling culture medium, cultivation soil and / or plant cultivation medium using the control agent according to any one of [1] to

[11] ; the plant being selected from the group consisting of Brassicaceae plants; beans; corn; wheat variety; tubers and roots; and sugar beet; The disease is caused by a fungus selected from the group consisting of fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia, and fungi belonging to the genus Aphanomyces.

[0040]

[13] Use of a filamentous fungus belonging to the genus Talaromyces in the manufacture of a disease control agent for a plant selected from the group consisting of Brassicaceae plants; beans; maize; wheat variety; tubers and roots; and sugar beet; wherein the disease is caused by a fungus selected from the group consisting of fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia, and fungi belonging to the genus Aphanomyces. EFFECT OF THE INVENTION

[0041] According to the present invention, by treating seeds, seedlings, soil and / or the like of a plant selected from the group consisting of Brassicaceae plants; beans; maize; wheat variety; tubers and roots; and sugar beet; with a filamentous fungus belonging to the genus Talaromyces, a high control effect can be produced against diseases such as damping-off. Petition 870240016976, dated 29 / 02 / 2024, page 21 / 80 8 / 28 caused by fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia, fungi belonging to the genus Aphanomyces and similar fungi.

[0042] In cases where the filamentous fungal strain belonging to the genus Talaromyces used is a fungal strain isolated from a natural source, for example, where the strain is Talaromyces flavus Y-9401, disease control is possible without adversely affecting the environment or the human body. Talaromyces flavus strains, such as strain Y-9401, are especially useful since their spores exhibit high storage stability and a plurality of formulations, including wettable powders, granules, and fluid formulations, can be selected for this purpose. MODALITIES FOR CARRYING OUT THE INVENTION<Fungos filamentosos pertencentes ao gênero Talaromyces>

[0043] The filamentous fungi belonging to the genus Talaromyces used in the disease control agent of the present invention are not particularly limited and are Talaromyces flavus, Talaromyces bacillisporus and Talaromyces helicam, Talaromyces luteus and Talaromyces rutundus. Talaromyces flavus is preferred.

[0044] In the case of Talaromyces flavus, the strain Talaromyces flavus Y-9401 is preferably mentioned. The strain Talaromyces flavus Y-9401 was released on September 2, 1996 by the Patent Microorganism Depository Center, Institute of Biotechnology and Industrial Technology, Ministry of International Trade and Industry (currently the National Institute of Technology and Evaluation, Biotechnology Center, Patent Microorganism Depository Center (currently the National Institute of Technology and Evaluation, Biotechnology Center). It was deposited at IPOD)) as FERM P-15816, transferred to an international deposit based on the Budapest Treaty on July 18, 2006, and given the accession number FERM BP-10642. Petition 870240016976, dated 29 / 02 / 2024, page 22 / 80 9 / 28<Agente de controle de pragas>

[0045] The disease control agent of the present invention contains the aforementioned filamentous fungus Talaromyces as the active ingredient.

[0046] The morphology of filamentous fungi of the genus Talaromyces is not particularly limited, as long as it can exert a disease control effect and can be any form of conidia, hyphae, asci, spores, etc. Spores are preferentially used.

[0047] The filamentous fungi Talaromyces can be cultivated in the same way as common filamentous fungi.

[0048] For example, filamentous fungi belonging to the genus Talaromyces can be cultivated by a liquid culture method, such as reciprocal liquid culture or jar fermentation culture, a semi-solid culture method of culturing in a semi-solid medium, or a solid culture method of culturing in a solid medium.

[0049] In cases where filamentous fungi belonging to the genus Talaromyces are subjected to liquid culture, potato-dextrose medium or Sabouraud medium can be used for this purpose. In cases where semi-solid culture is performed, a medium prepared by gelling potato-dextrose medium or Sabouraud medium can be used, or a medium prepared by suspending a cereal such as rice; a variety of wheat; corn; or soybeans; or by suspending a solid component derived from a cereal, such as soybean bran or flour; in water, can be used. In cases where solid culture is performed, a medium containing, for example: a grain such as rice; a variety of wheat; corn; or soybeans; a solid component derived from a cereal, such as soybean bran or flour; or a solid carrier, such as a clay mineral containing a source of nutrients; whose medium also contains, when necessary, a saccharide, a nitrogen source or similar, may be used.

[0050] The culture conditions for filamentous fungi belonging to the genus Talaromyces are preferably aerobic conditions by methods such as aeration, Petition 870240016976, dated 29 / 02 / 2024, page 23 / 80 10 / 28 agitation and agitation, and the culture temperature is preferably 20 to 40 °C. The culture period is preferably 3 to 60 days, more preferably 3 to 20 days.

[0051] The culture containing the filamentous fungus belonging to the genus Talaromyces can be used as a disease control agent, or it can be homogenized or chopped before use when necessary. Alternatively, mainly spores can be collected from the culture by sieving or similar means, and the collected product can be used. Alternatively, fungal cells can be separated from the culture using a liquid, such as water or oil, and the separated cells can be used as is or after concentration.

[0052] The amount of filamentous fungus Talaromyces contained in the disease control agent of the present invention is not particularly limited and can be adjusted depending on the dosage form. For example, in the case of a liquid preparation, 1 χ 10⁶ to 1 χ 10¹² cfu (colony forming unit) / g is preferable, more preferably 1 χ 10⁷ to 1 χ 10¹¹ cfu / g, and in the case of a solid agent, preferably 1 χ 10⁶ to 1 χ 10¹² cfu (colony forming unit) / g, more preferably 1 χ 10⁷ to 1 χ 10¹¹ cfu / g.

[0053] Provided that the growth, storage stability and disease control effect of the filamentous fungus belonging to the genus Talaromyces are not inhibited, the disease control agent of the present invention may contain, when necessary, an arbitrary component, such as an excipient or a surfactant, for formulation, quality stabilization and / or similar purposes. Examples of the arbitrary component used in the disease control agent of the present invention include one or more of the following components.

[0054] In the case of a solid vehicle, examples of the excipient include: fine mineral powders, such as kaolin clay, pyrophyllite clay, bentonite, montmorillonite, diatomaceous earth, synthetic hydrated silicon oxide, acid clay, talc, clay, ceramics, quartz, sericite, vermiculite, perlite, Oya stone, anthracite, limestone, coal, and zeolite; inorganic compounds, such as common salt, carbonates, sulfates, nitrates, and Petition 870240016976, dated 29 / 02 / 2024, p. 24 / 80 11 / 28 Urea; fine organic powders, such as chaff, bran, crab shells, shrimp shells, krill powder, rice husks, wheat flour, corn cobs, peanut shells, bone meal, fish meal, powdered husks, wood flour, charcoal, kuntan charcoal, husk charcoal, rice husk charcoal, vegetable / wood charcoal, peat, attapulgite, dried manure, activated charcoal, oil and starch cake and their hydrolysates; and soluble excipients, such as D-sorbitol, lactose, maltitose, glucosamine and oligosaccharides. In the case of a liquid vehicle, examples of excipients include: water, vegetable oils, animal oils, mineral oils and water-soluble synthetic polymers. One or more of these may be used.

[0055] Examples of surfactants include fatty acid soap, alkyl ether carboxylic acid, N-acylamino acid, alkylbenzenesulfonate, alkylnaphthalenesulfonate, αolefin sulfonate, dialkyl sulfosuccinate ester salt, higher alcohol sulfate ester salt and the like.Alkyl ether sulfate, polyoxyethylene alkylphenyl ether sulfate, fatty acid alkylolamide sulfate, alkyl ether phosphate, alkyl phosphate, aliphatic amine salt, quaternary aliphatic ammonium salt, benzalkonium salt, benzethonium chloride, pyridinium salt, alkyl imidazolinium salt, polyoxyethylene alkyl ether, polyoxyethylene alkyl ether, block polymer polyoxyethylene polyoxypropylene, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene glycerin fatty acid ester, polyoxyethylene castor oil, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitol fatty acid ester, polyethylene glycol fatty acid ester, fatty acid monoglyceride, polyglycerin fatty acid ester, sorbitan ester acid, fatty acid alkanolamide, fatty acid amide polyoxyethylene, polyoxy. Examples of these include ethylene alkylamine, alkylamine oxide, carboxybetaine, imidazolinium aminocarboxylate and betaine.One or more of these can be used.

[0056] When a surfactant and an excipient are used, it is preferable to contain 1 to 90% by mass of filamentous fungi belonging to the genus Talaromyces, 1 to 15% by mass of surfactant and 0 to 98% by mass of excipient. It is more preferable to contain a filamentous fungus belonging to the genus Talaromyces in an amount of 2 to 46% by mass, a surfactant in an amount of 2 to 15% by mass and an excipient. Petition 870240016976, dated 29 / 02 / 2024, page 25 / 80 12 / 28 excipient in an amount of 39 to 96% by mass.

[0057] Because Talaromyces spores are hydrophobic, it is effective to add a surfactant to improve dispersibility when diluting with water for treatment. If the amount of surfactant is too small, the dispersing effect cannot be obtained, and if the amount is too large, the effect plateaus and it is pointless to continue adding it, so the above range is preferable. By altering the amount of excipient used within the range, adjustment of the active ingredient concentration is possible.

[0058] In addition, when necessary, the agent may contain an adjuvant, such as a natural or similar polysaccharide, for example, casein, gelatin, gum arabic, alginic acid, cellulose, carboxymethylcellulose, xanthan gum, chitin or chitosan; a polyvinyl alcohol; a polyacrylate; or bentonite; for the purpose of thickening, adhesion, dispersion and / or the like.

[0059] In addition, when necessary, the agent may contain a dihydric alcohol or similar, for example, ethylene glycol or propylene glycol, for the purpose of preventing freezing and / or similar.

[0060] In addition, when necessary, the agent may contain a surfactant, such as an anionic surfactant, a cationic surfactant or a surfactant

[0061] amphoteric, for purposes of dispersion stabilization, aggregation inhibition, emulsification and / or the like.

[0062] The disease control agent of the present invention containing a filamentous fungus belonging to the genus Talaromyces can be formulated according to a common formulation production method, in a powder, granule, emulsion, wettable powder, fluid formulation, coating agent or the like together with arbitrary component(s) as required.

[0063] The wettable powder or powder can be produced by mixing, or pulverizing and mixing, as necessary, the surfactant described above and / or a component(s) for quality stabilization, with the solid vehicle described above.

[0064] The granule can be produced by mixing, or pulverizing and mixing, as needed, the surfactant described above and / or a component(s) for Petition 870240016976, dated 29 / 02 / 2024, page 26 / 80 13 / 28 stabilization of quality, with the solid vehicle described above, followed by granulation.

[0065] The emulsion can be produced by mixing or spraying and mixing, when necessary, the surfactant described above and / or a component(s) for quality stabilization, with a liquid carrier, such as a vegetable oil, an animal oil or a mineral oil.

[0066] The fluid formulation can be produced by mixing, or spraying and mixing, as needed, the adjuvant described above, dihydrogen alcohol and / or similar, surfactant and / or a component(s) for quality stabilization, with water.

[0067] The coating agent can be produced by adding an adjuvant to a liquid vehicle, such as water or oil, and then mixing the resulting mixture in the form of a sol or gel. <Plantas-alvo e doenças-alvo>

[0068] The plant to which the disease control agent of the present invention is to be applied is a Brassicaceae plant; bean; corn; wheat variety; tubers and roots; or sugar beet. Examples of Brassicaceae plants include broccoli, cabbage, cauliflower, kale, turnip, Chinese cabbage (hakusai), Japanese radish (daikon), Japanese horseradish (wasabi), and watercress. Examples of beans include soybeans, peas, common beans, azuki beans, broad beans, and chickpeas. Examples of wheat varieties include wheat, barley, and rye. Examples of tubers and roots include potato, sweet potato, taro (satoimo), Japanese yam (yamanoimo), and konjac tuber (konnyakuimo).

[0069] The target disease is one or more diseases caused by a fungus / fungus selected from the group consisting of fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, fungi belonging to the genus Pythium, fungi belonging to the genus Microdochium, fungi belonging to the genus Tilletia, fungi belonging to the genus Ustilago, fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia and fungi belonging to the genus Aphanomyces. Petition 870240016976, dated 29 / 02 / 2024, page 27 / 80 14 / 28

[0070] Examples of fungi belonging to the genus Rhizoctonia include Rhizoctonia solani, and examples of diseases caused by fungi belonging to the genus Rhizoctonia include numbness.

[0071] Examples of fungi belonging to the genus Fusarium include Fusarium solani and Fusarium oxysporum, and examples of diseases caused by fungi belonging to the genus Fusarium include damping-off.

[0072] Examples of fungi belonging to the genus Pythium include Pythium ultimum, Pythium aphanidermatum, Pythium spinosum, Pythium debaryanum, Pythium cucurbitacearum, and Pythium myriotylum, and examples of diseases caused by fungi belonging to the genus Pythium include damping-off.

[0073] Examples of fungi belonging to the genus Microdochium include Microdochium nival, and examples of diseases caused by fungi belonging to the genus Microdochium include pink snow mold.

[0074] Examples of fungi belonging to the genus Tilletia include Tilletia caries, Tilletia tritici, Tilletia leavis, and Tilletia foetida, and examples of diseases caused by fungi belonging to the genus Tilletia include stinking smut.

[0075] Examples of fungi belonging to the genus Ustilago include Ustilago nuda, and examples of diseases caused by fungi belonging to the genus Ustilago include smut.

[0076] Examples of fungi belonging to the genus Streptomyces include Streptomyces scabiei, and examples of diseases caused by fungi belonging to the genus Streptomyces include scabies.

[0077] Examples of fungi belonging to the genus Erwinia include Erwinia carotovora and Erwinia chrysanthemi, and examples of diseases caused by fungi belonging to the genus Erwinia include blackleg.

[0078] Examples of fungi belonging to the genus Aphanomyces include Aphanomyces cochlioides, and examples of diseases caused by fungi belonging to the genus Aphanomyces include damping-off and black root rot.

[0079] In cases where the disease control agent of the present invention is applied to Brassicaceae plants, the agent has an excellent control effect, Petition 870240016976, dated 29 / 02 / 2024, page 28 / 80 15 / 28 especially against diseases caused by fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium and fungi belonging to the genus Pythium.

[0080] In cases where the disease control agent of the present invention is applied to beans, such as soybeans, the agent has an excellent control effect, especially against diseases caused by fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium, and fungi belonging to the genus Pythium.

[0081] In cases where the disease control agent of the present invention is applied to corn, the agent has an excellent control effect, especially against diseases caused by fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium and fungi belonging to the genus Pythium.

[0082] In cases where the disease control agent of the present invention is applied to the wheat variety, the agent has an excellent control effect, especially against diseases caused by fungi belonging to the genus Ustilago, fungi belonging to the genus Tilletia, fungi belonging to the genus Microdochium, fungi belonging to the genus Rhizoctonia, fungi belonging to the genus Fusarium and fungi belonging to the genus Pythium.

[0083] In cases where the disease control agent of the present invention is applied to tubers and roots, such as potatoes, the agent has an excellent control effect, especially against diseases caused by fungi belonging to the genus Streptomyces, fungi belonging to the genus Erwinia and fungi belonging to the genus Rhizoctonia.

[0084] In cases where the disease control agent of the present invention is applied to sugar beet, the agent has an excellent control effect, especially against diseases caused by fungi belonging to the genus Aphanomyces. <Método de aplicação>

[0085] The disease control agent of the present invention containing a filamentous fungus belonging to the genus Talaromyces is applied for the treatment of Petition 870240016976, dated 29 / 02 / 2024, page 29 / 80 16 / 28 seeds, seedlings, plant bodies (leaves, stems, roots and the like), seedling culture soil, seedling culture medium, cultivation soil, cultivation medium and / or the like of a Brassicaceae plant; beans; maize; wheat variety; tubers and roots; or sugar beet; with the aim of controlling the diseases described above. The application method is selected appropriately according to the mode of use, such as the formulation and the disease.

[0086] Examples of the method include seed immersion treatment, seed treatment, seed coating treatment, seed spraying treatment, soil spraying treatment, soil incorporation application, soil soaking application, seedling tray soaking application, base application, liquid spraying on the soil part, solid spraying on the above-ground part, and the like. These can be performed repeatedly or in combination.

[0087] The amount of the disease control agent of the present invention containing a filamentous fungus belonging to the genus Talaromyces to be applied may vary depending on the type of disease and the like. For example, in cases where seed soaking treatment is performed, the agent is preferably applied as a seed soaking liquid in which the preparation is diluted 10 to 1000 times (by mass), and the cell concentration thereof is generally 1 x 10³ to 1 x 10¹⁰ cfu, preferably 1 x 10⁴ to 1 x 10⁹ cfu per 1 ml of the soaking liquid.

[0088] In the case of seed powder coating treatment, it is preferable to apply 1 to 20% by mass of the preparation in relation to the mass of the seed, and the concentration of bacterial cells is generally 1 χ 103 to 1 χ 1010 cfu, preferably 1 χ 104 to 1 χ 109 cfu per 1 g of seed mass.

[0089] In the case of soil spraying, if the spraying is liquid per seedling growing box (e.g., area of ​​about 1800 cm2), it is preferable to apply 50 to 1000 ml, and the cell concentration is generally 1 x 103 to 1 x 1010 cfu per 1 ml of sprayed liquid. It is preferably 1 x 104 to 1 x 1010 cfu. When growing seedlings or Petition 870240016976, dated 29 / 02 / 2024, page 30 / 80 17 / 28 growing seedlings using a seedling growing box different from the seedling growing box, the same application rate per unit area can be applied.

[0090] When the soil is mixed and applied, it is preferable to apply 0.1 to 100 ga per nursery box (e.g., an area of ​​about 1800 cm2), and the cell concentration thereof is 1 χ 102 to 1 χ 109 cfu per 1 ml of soil, preferably 1 x 103 to 1 x 108 cfu. When growing seedlings or growing seedlings using a seedling growing box different from the seedling growing box, the same application rate per unit area can be applied.

[0091] In cases of soil soaking application, when a liquid is sprayed onto a seedling tray (with an area of, for example, about 1800 cm2), the agent is preferably applied in a quantity of 50 to 1000 ml, and the fungal concentration is generally 1 x 103 to 1 x 109 cfu, preferably 1 x 104 to 1 x 108 cfu per 1 ml of spray liquid. Also, in cases where a material other than a seedling tray is used for the cultivation or growing of seedlings, the same quantity of the agent can be applied per unit area.

[0092] Provided that the growth and control effect of the filamentous fungus belonging to the genus Talaromyces are not adversely affected, the control agent of the present invention can be used as a mixture with, or in combination with, other disease control agents. For example, the agent can be used in combination with a microbicide, an insecticide, a nematicide, a miticide, a herbicide, a plant growth regulator, a fertilizer and / or a microbial agent. EXAMPLES

[0093] Hereafter, the present invention will be described in more detail with reference to the Examples, but the present invention is not limited to the aspects of these Examples.

[0094] Test on the control effect against cabbage damping-off caused by Rhizoctonia<Exemplo de produção 1> Petition 870240016976, dated 29 / 02 / 2024, page 31 / 80 18 / 28 (Spore production)

[0095] Wheat bran was used as a medium. An inoculum of the Talaromyces flavus Y-9401 strain was inoculated into the medium and the solid culture was carried out at 30 °C for 10 days. After completion of the culture, the culture was dried to obtain a powder containing spores of the Talaromyces flavus Y-9401 strain (4 χ 109 cfu / g). (Preparation manufacturing)

[0096] Using Talaromyces flavus Y-9401 spore-containing powder obtained in Production Example 1 above, 10% by mass of this spore-containing powder and 10% by mass of SORPOL4315L (manufactured by Toho Chemical Industry Co., Ltd.) as a surfactant. As an excipient, soybean oil (manufactured by Nisshin Oillio) was mixed and stirred to a mass proportion of 80% and filtered through an 180 μm sieve. The filtrate obtained here was concentrated by centrifugation to obtain a fluid preparation (1 χ 109 cfu / g). <Exemplo 1> (Test on the control effect against cabbage damping-off caused by Rhizoctonia) (1) Inoculation of pathogenic fungi

[0097] A horticultural soil (Genkikun No. 1) containing a fertilizer component was mixed with Rhizoctonia solani fungi obtained by static culture in potato dextrose broth (PDB) medium, so that the fungi were contained at 1 / 50 by weight, to provide a growing soil contaminated with Rhizoctonia. (2) Treatment with agent

[0098] Twenty cabbage seeds (variety: Okina) were sown in a 6.5 cm diameter plastic cup filled with cultivation soil contaminated with Rhizoctonia. The fluid formulation prepared in the above preparation was diluted to 1 χ 107 cfu / ml, 4 χ 106 cfu / ml and 2 χ 106 cfu / ml with water and uniformly sprayed (9.3 ml) onto the soil surface to provide agent treatment groups. In addition, a wettable powder (Rizolex wettable powder) containing tolclofos-methyl as the active ingredient was diluted 500 times with water and uniformly sprayed (9.3 ml) onto the soil surface to provide a group of Petition 870240016976, dated 29 / 02 / 2024, page 32 / 80 19 / 28 agent treatment control. In addition, a jar was prepared using water instead of the agent solution to provide a control (untreated group). Three independent tests were performed for the agent treatment groups and the untreated group. (3) Cultivation of test plants

[0099] After covering the plastic cups subjected to the above treatment with soil contaminated with Rhizoctonia, cultivation and management were carried out in a glass greenhouse according to a standard method. (4) Lifting the control effect

[0100] One week after sowing, seeds that did not sprout and seedlings with symptoms of damping-off caused by Rhizoctonia fungi, such as rot or wilting, were considered diseased seedlings, and the rate of healthy seedlings in each treatment group was calculated. Results

[0101] As shown in Table 1, it was found that the damping caused by Rhizoctonia solani can be controlled by treating the soil with the agent Talaromyces. Table 1 Untreated group inoculated Untreated group Non-inoculated group 1 x 107 cfu / ml 4 x 106 cfu / ml 2 x 106 cfu / ml Rizolex x 500 Number of seeds 20 20 20 20 20 20 Number of healthy seedlings I 15 20 19 18 18 19 II 9 18 18 19 17 18 III 7 20 18 17 15 20 Average 10.3 19.3 18.3 10.0 16.7 19.0 Ratio of healthy seedlings 51.7% 96.7% 91.7% 90.0% 83.3% 95.0% Petition 870240016976, dated 29 / 02 / 2024, p. 33 / 80 20 / 28<Exemplo de Produção 2> (Spore production)

[0102] Wheat bran was used as the medium. An inoculum of the Talaromyces flavus Y-9401 strain was inoculated into the medium and the solid culture was carried out at 30 °C for 10 days. After completion of the culture, the culture was dried, and a mixture of a clay mineral (Kaolin KH, Kanaya Kosan) as an excipient and the dried culture, prepared in a weight ratio of 1:9, was subjected to sieving to remove bran residues, to obtain a powder containing spores of the Talaromyces flavus Y-9401 strain (1 x 1010 cfu / g). <Exemplo de Formulação 2> (Formulation production)

[0103] The powder containing Taralomyces flavus Y-9401 spores obtained in Production Example 2 above was used. A mixture containing this spore-containing powder at 20% by mass, SORPOL5082 (manufactured by Toho Chemical Industry Co., Ltd.) as a surfactant at 10% by mass, mineral clay (Kaolin KH) as an excipient at 38% by mass, glucosamine (manufactured by Protein Chemical) at 30% by mass, and Sumecton SA (manufactured by Kunimine Kogyo Co., Ltd.) as a thickener at 2% by mass was prepared, and the mixture was blended and pulverized using a mill grinder to obtain a wettable powder of Formulation Example 2 (2 x 109 cfu / g). <Exemplo de Produção 3> (Spore production)

[0104] Wheat bran was used as a medium. An inoculum of the Talaromyces flavus Y-9401 strain was inoculated into the medium and the solid culture was carried out at 30 °C for 10 days. After completion of the culture, the culture was dried, and the dried culture was subjected to sieving to remove wheat bran residues, to obtain a powder containing Talaromyces flavus Y-9401 spores (4 x 109 cfu / g). <Formulation Example 3> (Formulation Production)

[0105] The powder containing Talaromyces flavus Y-9401 spores obtained above in Petition 870240016976, dated 29 / 02 / 2024, page 34 / 80 21 / 28 Example Formulation 3 was used. A mixture containing this spore-containing powder at 10% by mass, SORPOL5082 (manufactured by TOHO Chemical Industry Co., Ltd.) as a surfactant at 5% by mass, mineral clay (HA-A Kaolin Clay, manufactured by Maruo Calcium Co., Ltd.) as an excipient at 45% by mass, and glucosamine (manufactured by Protein Chemical Co., Ltd.) at 40% by mass was prepared and the mixture was blended and pulverized using a grinding mill, to obtain a wettable powder of Example Formulation 3 (4 χ 108 cfu / g). <Exemplo 2> (Test on the control effect against wheat damping-off caused by Pythium) (1) Inoculation of pathogenic fungus

[0106] Pythium ultimum were subjected to shake culture in liquid cornmeal medium. A medium was prepared by mixing soil and bran in a volume ratio of 2:1, and adjusting the water content to 50%, followed by sterilization by autoclaving. The cultured pathogens were inoculated into the prepared medium and the solid culture was carried out for seven days. The resulting culture was mixed in a volume ratio of 1:4 with autoclave-sterilized soil to provide a contaminated soil. (2) Treatment with Agent

[0107] Ten wheat seeds (variety: Norin no. 61) were sown in a 5.5 cm diameter plastic cup filled with soil contaminated with Pythium ultimum. The sown seeds were completely covered with soil. The wettable powder prepared in Formulation Example 2 was diluted to 2 χ 10⁸ cfu / ml, 7 χ 10⁷ cfu / ml or 2 χ 10⁷ cfu / ml with water and sprayed uniformly (15 ml) onto the soil surface to provide agent treatment groups. In addition, for comparison, wheat seeds were subjected to a 0.4% by weight treatment with a wettable powder (Orthocide 80 wettable powder) containing Captan as the active ingredient and then sown to provide an agent treatment control group. Furthermore, an untreated inoculation group, in which no treatment with the agent solution was performed, and a non-treated inoculation group were also included. Petition 870240016976, dated 29 / 02 / 2024, page 35 / 80 22 / 28 untreated inoculation, in which 10 wheat seeds were sown in sterilized healthy soil, were provided. For all test groups, three independent tests were performed. (3) Cultivation of test plants

[0108] Each plastic cup subjected to the above treatment was placed in a greenhouse, and cultivation management was carried out according to a conventional method. (4) Investigation of the Control Effect

[0109] Two weeks after sowing, seeds without sprouting and seedlings with symptoms of damping-off caused by Pythium, such as rot or wilting, were considered diseased seedlings and the rate of healthy seedlings in each treatment group was calculated. Results

[0110] As shown in Table 2, it was found that the damping caused by Pythium ultimum can be controlled by treatment with the agent Talaromyces. Table 2 Untreated group inoculated Untreated group Non-inoculated group 2 x 10⁸ cfu / ml 7 x 10⁷ cfu / ml 2 x 10⁷ cfu / ml Orthocide 0.4% Number of seeds 10 10 10 10 10 10 Number of healthy seedlings I 1 10 9 9 7 10 II 0 9 10 8 7 6 III 4 10 10 10 9 8 Average 1.7 9.7 9.7 9.0 7.7 8.0 Seedling ratio 16.7% 96.7% 96.7% 90.0% 76.7% 80.0% Petition 870240016976, dated 29 / 02 / 2024, page 36 / 80 23 / 28 healthy <Exemplo 3> (Test on the control effect against dent corn damping caused by Pythium) (1) Inoculation of pathogenic fungus

[0111] The culture prepared in Example 2 was mixed in a volume ratio of 1:9 with autoclave-sterilized soil to provide contaminated soil. (2) Treatment with Agent

[0112] Five dent maize seeds (variety: 34N84) were sown in a 5.5 cm diameter plastic cup filled with soil contaminated with Pythium ultimum. The sown seeds were completely covered with soil. The wettable powder prepared in Formulation Example 2 was diluted to 2x10⁸ cfu / ml, 7x10⁷ cfu / ml or 2x10⁷ cfu / ml with water and sprayed uniformly (15 ml) onto the soil surface to provide agent treatment groups. In addition, for comparison, dent maize seeds were subjected to a 0.4% by weight topdressing treatment with a wettable powder (Orthocide 80 wettable powder) containing Captan as the effective ingredient and then sown to provide an agent treatment control group. In addition, an untreated inoculation group, in which no treatment with the agent solution was carried out, and an untreated non-inoculation group, in which five dent corn seeds were sown in sterilized healthy soil, were provided.For all test groups, three independent tests were performed. (3) Cultivation of test plants

[0113] Each plastic cup subjected to the above treatment was placed in a greenhouse, and cultivation management was carried out according to a conventional method. (4) Investigation of the Control Effect

[0114] Two weeks after sowing, seeds without sprouting and seedlings with symptoms of damping-off caused by Pythium, such as rot or wilting, Petition 870240016976, dated 29 / 02 / 2024, page 37 / 80 24 out of 28 were considered diseased seedlings, and the rate of healthy seedlings in each treatment group was calculated. Results

[0115] As shown in Table 3, it was found that the damping caused by Pythium ultimum can be controlled by treatment with the agent Talaromyces. Table 3 Untreated group inoculated Untreated group non-inoculated group 2 x 10⁸ cfu / ml 7 x 10⁷ cfu / ml 2 x 10⁷ cfu / ml Orthocide 0.4% Number of seeds 5 5 5 5 5 5 Number I 0 4 2 1 1 5 of II 0 4 4 2 0 1 healthy seedlings III 0 5 1 2 0 2 Average 0.0 4.3 2.3 1.7 0.3 2.7 Ratio of healthy seedlings 0.0% 86.7% 46.7% 33.3% 6.7% 53.3% <Exemplo 4> (Test on the control effect against soybean damping-off caused by Fusarium) (1) Inoculation of pathogenic fungus

[0116] Fusarium oxysporum fungi were subjected to static culture in potato dextrose agar (PDA medium). A medium was prepared by mixing soil and cornmeal in a 5:1 volume ratio, and adjusting the water content to 50%, followed by Petition 870240016976, dated 29 / 02 / 2024, pp. 38 / 80 25 / 28 autoclave sterilization. The cultured pathogens were inoculated into the prepared medium and the solid culture was carried out for 21 days. The resulting culture was mixed in a volume ratio of 1:4 with autoclave-sterilized soil to provide a contaminated soil. (2) Treatment with Agent

[0117] Five soybean seeds (variety: Enrei) were sown in a 5.5 cm diameter plastic cup filled with soil contaminated with Fusarium oxysporum. The sown seeds were completely covered with soil. The wettable powder prepared in Formulation Example 2 was diluted to 2 x 10⁸ cfu / ml or 2 x 10⁷ cfu / ml with water and uniformly sprayed (15 ml) onto the soil surface to provide agent treatment groups. In addition, for comparison, soybean seeds were subjected to a 0.5% by weight topdressing treatment with a wettable powder (Homai wettable powder) containing thiuram and thiophanate-methyl as effective ingredients and then sown to provide an agent treatment control group. In addition, an untreated inoculation group was provided, in which no treatment with the agent solution was carried out, and a non-treated inoculation group, in which five soybean seeds were sown in sterilized healthy soil.For all test groups, three independent tests were performed. (3) Cultivation of test plants

[0118] Each plastic cup subjected to the above treatment was placed in a greenhouse, and cultivation was carried out according to a conventional method. (4) Investigation of the Control Effect

[0119] Two weeks after sowing, seeds without sprouting and seedlings with symptoms of damping-off caused by Fusarium fungi, such as rot or wilting, were considered diseased seedlings and the rate of healthy seedlings in each treatment group was calculated. Results

[0120] As shown in Table 4, it was found that the damping caused by Fusarium oxysporum can be controlled by soaking treatment of Petition 870240016976, dated 29 / 02 / 2024, page 39 / 80 26 / 28 solo with the agent Talaromyces. Table 4 Untreated group inoculated Untreated group non-inoculated group 2 x 10⁸ cfu / ml 2 x 10⁷ cfu / ml Orthocide 0.4% Number of seeds 5 5 5 5 5 Number of healthy seedlings I 0 4 1 3 2 II 1 3 3 2 1 III 1 4 3 1 0 Average 0.7 3.7 2.3 2.0 1.0 Ratio of healthy seedlings 13.3% 73.3% 46.7% 40.0% 20.0% <Exemplo 5> (Potato crust control effect test) (1) Agent Treatment

[0121] A liquid agent was prepared by diluting the wettable powder prepared above in Formulation Example 3 to 1 x 10⁶ cfu / ml. Contaminated potatoes (variety: Nishiyutaka) infected with Streptomyces scabiei, having approximately 4 to 10 lesions per seed potato, were soaked for 1 minute or 10 seconds in the prepared liquid agent. After drying the liquid agent, the seed potatoes were sown in the field. Additionally, for comparison, a liquid agent was prepared by diluting a wettable powder (Agrimycin 100 wettable powder) containing streptomycin and oxytetracycline as effective ingredients 100 times, and potatoes were soaked in it for 10 seconds, followed by drying, to provide an agent treatment control group. Furthermore, an untreated inoculation group was provided by immersion in water only followed by drying. For all test groups, three independent tests were performed. (2) Investigation of the Control Effect Petition 870240016976, dated 29 / 02 / 2024, pp. 40 / 80 27 / 28

[0122] Potatoes were harvested 81 days after sowing. Those exhibiting scab lesions on the stem-tuber surface were considered diseased stem tubers, and the rate of diseased stem tubers relative to the total number of stem tubers was calculated. Results

[0123] As shown in Table 5, it was found that crusting caused by Streptomyces scabiei can be controlled by treatment with the agent Talaromyces. Table 5 No. Treatment Yield of diseased stem tubers Total number of stem tubers Ratio of diseased stem tubers Number Number I 1 x 10⁶ cfu / ml Absorbing 1 minute 75 126 59.5% II 53 105 50.5% III 56 119 47.1% Average 61.3 116.7 52.4% I 1 x 10⁶ cfu / ml Absorbing 10 seconds 45 102 44.1% II 40 82 48.8% III 35 96 36.5% Average 40.0 93.3 43.1% I Agrimicyn Absorbing 10 seconds 44 79 55.7% II 25 64 39.1% III 37 82 45.1% Average 35.3 75.0 46.6% I Group inoculation without 87 98 88.8% II 90 124 72.6% III 97 132 73.5% Petition 870240016976, dated 29 / 02 / 2024, page 41 / 80 28 / 28 Treatment Average 91.3 118.0 78.3%

Claims

CLAIMS 1. Use of filamentous fungi belonging to the genus Talaromyces, characterized by the fact that it is in the manufacture of a disease control agent for a plant, wherein when said plant is cabbage, said disease is caused by fungi belonging to the genus Rhizoctonia; when said plant is corn, said disease is caused by fungi belonging to the genus Pythium; when said plant is a variety of wheat, said disease is caused by fungi belonging to the genus Pythium; when said plant is soybean, said disease is caused by fungi belonging to the genus Fusarium; and wherein Talaromyces flavus is the Talaromyces flavus Y-9401 strain (FERM BP-10642).

2. Use, according to claim 1, characterized in that said control agent comprises the Talaromyces flavus Y-9401 strain in an amount of 1 χ 106 to 1 χ 1012 colony forming units / g.

3. Use, according to any one of claims 1 to 2, characterized in that said control agent further comprises a surfactant and / or an excipient.

4. Use according to claim 3, characterized in that the surfactant is one or more surfactants selected from the group consisting of fatty acid soaps, alkyl ether carboxylic acid, N-acylamino acid, alkyl benzene sulfonate, alkyl naphthalene sulfonate, alpha-olefin sulfonate, dialkyl sulfosuccinate salt, higher alcohol sulfate salt, alkyl ether sulfate, polyoxyethylene alkylphenyl ether sulfate, fatty acid amide alkylol sulfate salt, alkyl ether phosphate salt, alkyl phosphate salt, aliphatic amine salt, quaternary aliphatic ammonium salt, benzalkonium chloride, benzethonium chloride, pyridinium salt, imidazolinium salt, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene polyoxypropylene block polymer, polyoxyethylene alkyl ether. 870260031689, dated 06 / 04 / 2026, page.8 / 15 2 / 3 polyoxypropylene, polyoxyethylene fatty acid ester glycerin, castor oil polyoxyethylene, polyoxyethylene fatty acid ester sorbitan, polyethylene fatty acid ester sorbitol ester, polyethylene glycol fatty acid ester, fatty acid monoglyceride, polyglycerin fatty acid ester, sorbitan fatty acid ester, fatty acid alkanolamide, polyoxyethylene fatty acid amide, polyoxyethylene alkylamine, alkylamine oxide, carboxybetaine, aminocarboxylate and imidazolinium betaine.

5. Use, according to claim 3 or 4, characterized in that the excipient is one or more excipients selected from the group consisting of: fine mineral powders selected from the group consisting of kaolin clay, pyrophyllite clay, bentonite, montmorillonite, diatomaceous earth, synthetic hydrated silicon dioxide, acid clay, talc, clay, ceramic, quartz, sericite, vermiculite, perlite, Oya stone, anthracite, limestone, coal and zeolite; inorganic compounds selected from the group consisting of common salt, carbonates, sulfates, nitrates and urea;Selected organic materials from the group consisting of tares, bran, crab shells, shrimp shells, krill powder, rice bran, wheat flour, corn cobs, peanut shells, bone meal, fish meal, powdered tar, wood flour, charcoal, kuntan charcoal, tree bark charcoal, rice husk charcoal, vegetable / wood charcoal, peat, attapulgite, dried manure, activated charcoal, oil and starch cake and their hydrolysates; selected saccharides from the group consisting of D-sorbitol, lactose, maltitose, glucosamine and oligosaccharides; selected oils from the group consisting of vegetable oils, animal oils and mineral oils; and water-soluble synthetic polymers.

6. Use, according to any one of claims 3 to 5, characterized in that said control agent comprises the Talaromyces flavus Y-9401 strain in 1 to 90% by mass, the surfactant in 1 to 15% by mass and the excipient in 0 to 98% by mass. Petition 870260031689, dated 06 / 04 / 2026, page 9 / 15 3 / 3 7. Use, according to any one of claims 3 to 5, characterized in that said control agent comprises the Talaromyces flavus Y-9401 strain at 2 to 46% by mass, the surfactant at 2 to 15% by mass and the excipient at 39 to 96% by mass.

8. Use, according to any one of claims 1 to 7, characterized in that said control agent is formulated as any one of a powder, a granule, an emulsion, a wettable powder, a fluid formulation, and a coating agent.

9. A method for controlling a plant disease, characterized by the fact that it comprises: treatment of seeds, seedlings, plant bodies, seedling culture soil, seedling culture medium, cultivation soil and / or plant cultivation medium, using a control agent comprising the strain Talaromyces flavus Y9401; when said plant is cabbage, said disease is caused by fungi belonging to the genus Rhizoctonia; when said plant is maize, said disease is caused by fungi belonging to the genus Pythium; when said plant is a variety of wheat, said disease is caused by fungi belonging to the genus Pythium; when said plant is soybean, said disease is caused by fungi belonging to the genus Fusarium.