Anti-wrinkle composition for skin care, comprising a stable homogeneous aqueous suspension of biopolymers, its uses, kit and cosmetic method.

Description

1 / 72 Anti-wrinkle composition for skin care, comprising a homogeneous aqueous suspension. Stable Biopolymers: Their Uses, Kit, and Cosmetic Method CROSS-REFERENCE TO RELATED REQUESTS

[001] The present application claims priority over provisional patent application US 63 / 485,000, filed on February 14, 2023, the content of which is incorporated herein in its entirety. FIELD OF THE INVENTION

[002] The present invention relates to the field of cosmetics and, more particularly, to skin care compositions with beneficial properties for the skin, including smoothing the appearance of fine lines and / or wrinkles. BACKGROUND OF THE INVENTION

[003] The cosmetics industry is constantly searching for new natural, biocompatible, biodegradable, and non-toxic ingredients. The cosmetics industry is also constantly searching for compositions with beneficial properties for human skin.

[004] Natural polymers, or biopolymers, are abundant, natural, and renewable polymers, making them an attractive resource for the cosmetics industry. However, most abundant biopolymers, such as cellulose and chitin, are insoluble, limiting and hindering their use. Advantageously, the Applicant has described, in the international publication PCT WO 2022 / 137184, novel stable and homogeneous suspensions of biopolymers that may find numerous applications in the cosmetics industry. The international patent application PCT / IB2023 / 056723, filed on June 29, 2023, which also belongs to the Applicant, describes cosmetic formulations comprising a biopolymer.

[005] However, there is still a need for compositions that Petition 870260082686, dated 08 / 14 / 2026, page 6 / 182 2 / 72 exhibit beneficial properties for skin care, including skin protection, smoothing the appearance of fine lines and / or wrinkles, lightening age spots, skin regeneration, etc.

[006] The present invention meets these and other needs, as will become evident from the analysis of the invention and the description of the invention's features that follow. BRIEF SUMMARY OF THE INVENTION

[007] According to one aspect, the invention relates to a skin care composition for application to human skin, wherein said composition comprises biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension, and wherein said stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent.

[008] According to a specific aspect, the invention relates to a skin care composition comprising: (i) biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension, wherein said stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent; and (ii) at least one of a humectant, an emollient, an occlusive, a ceramide, an emulsifier, a thickening agent, a preservative, an antioxidant, a fragrance, a functional ingredient, a UVB filter, a UVA filter and a broad-spectrum filter; and wherein said skin care composition is formulated for dermatologically safe application to human skin.

[009] According to another aspect, the invention relates to a skin care composition for application to human skin, wherein said composition comprises biopolymers Petition 870260082686, dated 08 / 14 / 2026, page 7 / 182 3 / 72 insoluble and / or semi-soluble substances that have been mechanically processed into a stable homogeneous aqueous suspension, and wherein said skin care composition provides at least one skin care benefit selected from the group consisting of: i. to soften the appearance of fine lines and / or wrinkles and / or be effective in reducing their presence; ii. improve the appearance of under-eye bags and facial wrinkles; iii. protect the skin; iv. to lighten age spots; v. to mattify the skin; vi. to regenerate and / or rejuvenate the skin; vii. to repair and / or heal the skin; viii. to thicken the skin and / or improve one or more mechanical properties of the skin; ix. improve skin elasticity; x. create a protective film on the skin, for example, a long-lasting protective film, lasting at least 24 hours, or at least one week, or at least ten (10) days; xi. to create a film on the skin that is almost dry to the touch; xii. to create a refreshing sensation on the skin; xiii. to hydrate and / or moisturize the skin; xiv. prevent water evaporation from the skin; xv. improve the integrity of the skin barrier; xvi. reduce epidermal water loss and / or improve water retention by the skin; and xvii. non-comedogenic.

[0010] According to another aspect, the invention relates to a method of protecting the skin, comprising application to the skin. Petition 870260082686, dated 08 / 14 / 2026, page 8 / 182 4 / 72 of a skin care composition, as defined herein.

[0011] According to another aspect, the invention relates to a method for treating skin wrinkles, comprising applying a composition, as defined herein, to the skin surface of a human individual in need thereof.

[0012] According to another aspect, the invention relates to the use of a composition, as defined herein, for at least one of the following purposes: prevention of the appearance of skin wrinkles and removal of skin wrinkles.

[0013] According to another aspect, the invention relates to the use of a composition, as defined herein, in the manufacture of a cosmetic for skin care.

[0014] According to another aspect, the invention relates to a skin care kit comprising two-part formulations that are activated when combined, wherein at least one of the two-part formulations comprises a skin care composition as defined herein.

[0015] Aspects, advantages and additional features of the present invention will become more apparent upon reading the following non-restrictive description of preferred embodiments, which are illustrative and should not be interpreted as limiting the scope of the invention. BRIEF DESCRIPTION OF THE FIGURES

[0016] To facilitate understanding of the invention, embodiments of the invention are illustrated by means of examples in the accompanying figures.

[0017] Figure 1: Images showing the application scheme and patterns for testing Example 2.

[0018] Figure 2: Images showing samples in the vial that were tested according to Example 2.

[0019] Figure 3: Table displaying visual and sensory scores Petition 870260082686, dated 08 / 14 / 2026, page 9 / 182 5 / 72 of samples 1 to 3, tested on the skin according to Example 2. The score ranges from 1 to 10, with 1 being the worst score and 10 the best.

[0020] Figure 4: Table displaying the visual and sensory scores of samples 4 to 6, tested on the skin according to Example 2. The score ranges from 1 to 10, with 1 being the worst score and 10 the best.

[0021] Further details of the invention and its advantages will be evident in the detailed description included below. DETAILED DESCRIPTION OF THE MODALITIES

[0022] In the following description of embodiments, references to accompanying figures are illustrations of an example by which the invention can be practiced. It is understood that other embodiments can be made without departing from the scope of the invention described. Unless otherwise defined, all technical and scientific terms used herein have the same meaning commonly understood by one with common knowledge of the art to which the invention pertains. Overview

[0023] In International PCT Publication WO 2022 / 137184 (incorporated herein by reference in its entirety), the Applicant describes the preparation of stable homogeneous suspensions of insoluble and / or semi-soluble biopolymers. In International Patent Application PCT / IB2023 / 056723 of June 29, 2023 (also incorporated herein by reference in its entirety), the Applicant describes cosmetic formulations comprising a biopolymer.

[0024] The present invention relates generally to the uses of such suspensions and / or formulations in the manufacture of skin care compositions or skin care formulations (both terms are used interchangeably herein) for use on human skin. Particular aspects relate to novel protective and / or skin care compositions that exhibit beneficial skin care properties, including, but not limited to, protecting Petition 870260082686, dated 08 / 14 / 2026, p. 10 / 182 6 / 72 skin conditioning, smoothing the appearance of fine lines and / or wrinkles, lightening age spots, skin regeneration, etc. Biopolymer

[0025] The skin care compositions according to the present invention require at least one biopolymer. As used herein, the term biopolymer refers to natural polymers produced by cells of living organisms. Biopolymers consist of monomeric units that are covalently linked to form larger molecules. The present invention encompasses polypeptides, polysaccharides, and polynucleotides – biopolymers. Other examples of biopolymers include natural rubbers (isoprene polymers), suberin and lignin (complex polyphenolic polymers), cutin and cutan (complex polymers of long-chain fatty acids), and melanin. In some embodiments, the biopolymers used as starting materials and obtained in the suspensions are substantially pure, i.e., consist only of purified natural polymers.

[0026] Preferably, the biopolymer used in the skin care formulation is substantially free of chemical residues, and any such chemical residues are absent or present in undetectable or trace amounts. Preferably, the biopolymer is substantially free of chemical residues, and any such chemical residues are absent or present in undetectable or trace amounts. As used herein, substantially free of chemical residues means that chemical compounds such as acids, bases, reactive chemicals, organic and / or inorganic salts, surfactants, dispersing agents (e.g., Tween 80™), a silanizing reagent, acrylamide, etc., are totally absent or present only in undetectable or trace amounts in the final composition or final suspension. In some embodiments, the biopolymer(s) will constitute at least 98%, or at least 99%, Petition 870260082686, dated 08 / 14 / 2026, page 11 / 1827 / 72 or at least 99.9%, or at least 99.99%, by weight, of the organic compounds in the biopolymer composition or suspension, that is, the biopolymer composition or suspension will contain less than 2%, or less than 1%, or less than 0.1%, or less than 0.01%, or less than 0.001%, by weight, of organic components other than the biopolymer(s) or degradation product(s). In some embodiments, the skin care composition according to the invention comprises biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension, but in the absence of chemical compounds such as acids, bases, or other chemicals typically used in the solubilization and / or mechanical processing of biopolymers.In other words, the skincare composition according to the invention comprises stably dispersed biopolymer molecules that were not obtained by solubilization in an acidic solution (e.g., pH < 5.5; acetic acid) or by solubilization in a basic solution (e.g., pH > 8.5; NaOH). In other embodiments, the skincare composition according to the invention comprises stably dispersed biopolymer molecules that were not solubilized due to chemical modifications such as carboxylation and / or alkylation. According to the present invention, the biopolymer may be insoluble or semi-soluble in water. As used herein, the term insoluble biopolymer refers to a biopolymer that is insoluble in a polar solvent (particularly water), and this term encompasses equivalent terms such as non-water-soluble, not soluble in water, insoluble in water, or indissoluble.Insolubility can typically be observed by a separation, i.e., two distinct phases in an aqueous mixture, for example, biopolymer deposits / sediments at the bottom or floating on the surface of the aqueous mixture. According to the present invention,... Petition 870260082686, dated 08 / 14 / 2026, p. 12 / 182 8 / 72 Examples of insoluble biopolymers include, but are not limited to, chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, fibroin, keratin, wool, alginic acid, and mixtures thereof. As used herein, the term semi-soluble biopolymer refers to a biopolymer that can be solubilized in a polar solvent, such as water, but under certain conditions (e.g., molecular weight, heat, addition of chemicals such as acids, alcohols, surfactants, etc.). According to the present invention, examples of semi-soluble biopolymers include, but are not limited to, gelatin, pectin, starch, amylopectin, agarose, hyaluronic acid, RNA, DNA, xanthan gum, latex, polymannans, suberin, cutin, cutan, and mixtures thereof.

[0027] In some embodiments, the insoluble biopolymer is selected from chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, fibroin, keratin, wool, and mixtures thereof. In other embodiments, the semi-soluble biopolymer is selected from gelatin, pectin, starch, amylopectin, agarose, alginic acid, alginate, hyaluronic acid, RNA, DNA, xanthan gum, guar gum, carrageenan, latex, polymannans, suberin, cutin, cutan, and mixtures thereof.

[0028] Various sources of biopolymers can be used, and the present invention is not limited to specific sources of materials. For example, suitable sources of chitin may include, among others, green plants, algae, and fungi. Suitable sources of chitin and chitosan may include, among others, fungi, crustaceans (e.g., crabs and shrimp), and insects. In some embodiments, the insoluble or semi-soluble biopolymer is obtained from fungi and mushrooms. In other embodiments, the insoluble or semi-soluble biopolymer is obtained from plant materials, including, among others, roots, tubers, leaves, petals, seeds, fruits, etc. Preferred sources are sources Petition 870260082686, dated 08 / 14 / 2026, page 13 / 182 9 / 72 non-animals.

[0029] In some embodiments, the biopolymer is a 100% natural biopolymer, such as SunSpheres Bio™ (microcrystalline cellulose, Dow Chemical), Chemjac™ (konjac amorphophallus root extract and xanthan gum, Chemspire), Kelset™ (sodium alginate, Dupont), Instant Pure-Flo F™ (corn starch, Ingredion), Gelcarin™ GP 379 (carrageenan, iota form, Dupont), Betafib™ MCF (cellulose and microfibrillated cellulose in water, Cosun Biobased Products), Betafib™ ETD (cellulose and microfibrillated cellulose gum, Cosun Biobased Products), Exilva™ FM02-V,L (microfibrillated cellulose, Borregard), Naturesoft™ 800 (micropowdered cellulose), Kelcogel™ CG-HA (gellan gum-CP, Kelco), agar-agar and agarose, mushroom chitosan (e.g., GBS003, Qingdao Chibio Biotech), fungal chitosan (e.g., GBS010, Qingdao Chibio Biotech; or from Kraeber & Co), a chitosan derived from fungi (e.g., Kiosmetine-CS™, Kitozyme).

[0030] In some embodiments, the biopolymer is a naturally derived biopolymer, such as Natrathix™ bio cellulose (cellulose gum, Ashland), Aquasorb™ A500 (cellulose gum, Ashland), Polysurf™ CS 67 / Natrosol™ CS plus 330 (cetyl hydroxyethylcellulose, Ashland), Structure XL™ (hydroxypropyl starch phosphate, Nouryon), CD-58 (chitosan succinimide, Onlystar Bio-Technology Ltd), carboxymethyl chitosan derivative (GBS010, Qingdao Chibio Biotech), Makimousse™ 7 / 400 (sodium polyacrylate starch products - kobo, Daito Kasei Kogyo), Salanjul / Sanfresh™ 1000 / 300sp (sodium polyacrylate starch, Iwase Cosfa USA Inc. / Sanyo), Antaron ECoT ethylcellulose (ethylcellulose, Ashland).

[0031] In some embodiments, the biopolymer comprises a synergistic combination of biopolymers, such as Chemjac™ (konjac amorphophallus root extract and xanthan gum, Chemspire), PemuPur™ start (microcrystalline cellulose and Sphin yeast extract). Petition 870260082686, dated 08 / 14 / 2026, p. 14 / 182 10 / 72 gums (and) cellulose gum, (Lubrizol)), Nomcort CG (xanthan gum, ceratonia siliqua gum, Ikeda).

[0032] The biopolymer may also comprise other biopolymeric actives, such as B-CAN™ 55% (oat beta glucan, Adams Food Ingredients) and / or mushroom-derived beta glucan.

[0033] The present invention encompasses mixtures of two, three, four, five or more insoluble biopolymers, including, but not limited to, chitin + chitosan, chitin + cellulose, chitin + collagen, chitin + silk, chitosan + silk, chitosan + cellulose, chitosan + collagen, cellulose + collagen, cellulose + silk, collagen + silk, etc. The present invention also encompasses mixtures of two, three, four, five or more semi-soluble biopolymers, including, but not limited to, agarose + DNA, xanthan gum + starch, latex + alginate, xanthan gum + DNA, guar gum + cutan, etc. It may also be foreseen that two, three, four, five or more insoluble and semi-solid biopolymers will be used, including, but not limited to, chitin + agarose, chitosan + agarose, chitin + gelatin, chitin + xanthan gum, chitosan + xanthan gum, chitin + sodium hyaluronate, chitosan + sodium hyaluronate, cellulose + sodium hyaluronate, chitin + agarose, chitosan + agarose, cellulose + agarose.

[0034] The present invention also encompasses combinations of hydrophobically modified biopolymers and unmodified biopolymers that can form viscous and stable oil-in-water emulsions when dispersed by means of high shear and / or mechanical energy processes (with or without emulsifiers). Examples of hydrophobically modified biopolymers include Natrosol™ CS Plus 330 / Polysurf™ CS 67 (cetyl hydroxyethylcellulose, Ashland), StarDesign Ultra™ (sodium starch octenylsuccinate, Cargill Beauty), Inutec™ SP1 (inulin lauryl carbamate, Beneo), Texturlux Stabil™ (hydrolyzed corn starch hydroxyethyl ether, Primient). Petition 870260082686, dated 08 / 14 / 2026, page 15 / 182 11 / 72

[0035] In some embodiments, the biopolymer consists of a biopolymer composition comprising biopolymer molecules that have been mechanically processed into a homogeneous and stable aqueous biopolymer suspension. As used herein, the term homogeneous generally refers to the appearance of the suspension to the naked eye (e.g., uniform color, uniform texture, etc.). Homogeneous, as used herein, does not exclude the possibility that the suspension is heterogeneous at the molecular level (e.g., various particle sizes, presence of aggregates, etc.). As used herein, the terms homogeneous and stable aqueous biopolymer suspension, or similar terms that may be used herein interchangeably, such as homogeneous biopolymer suspension or stable biopolymer suspension or simply biopolymer suspension, all refer to a suspension of insoluble and / or semi-soluble biopolymer particles that have been stably dispersed in a polar solvent.The polar solvent can be a protic polar solvent or an aprotic polar solvent. The polar solvent can be an aqueous solvent. The insoluble and / or semi-soluble biopolymer particles present in biopolymer suspensions can be in the form of agglomerated fibers and / or spheres or agglomerated bodies. The stability of the biopolymer suspensions can be evaluated by any suitable means. In preferred embodiments, stability is measured or observed by the absence of separation, i.e., a single phase instead of two separate phases in an aqueous mixture, for example, the absence of biopolymer deposits / sediments at the bottom or floating on top of the aqueous mixture. Preferably, the biopolymer suspensions according to the present invention are stable (e.g., absence of separation) for at least 1 day, or at least 1 week, or at least one month, or at least one year or more.

[0036] Notwithstanding the foregoing, those versed in the technique of Petition 870260082686, dated 08 / 14 / 2026, page 16 / 182 12 / 72 suggest that insoluble and / or semi-soluble biopolymers may never become truly soluble. Instead, they become swollen and retain water, which is why the biopolymer becomes a viscous suspension under the high shear and / or mechanical energy conditions to which the biopolymer(s) is / are subjected according to the present invention. Consequently, the present invention encompasses both swelling and non-swelling biopolymers, since a non-swelling polymer could become swelling using high shear and / or mechanical energy. As used herein, swelling biopolymers encompass biopolymers that absorb and retain water, resulting in an increase in their particle size and water dispersion viscosity.

[0037] In some embodiments, the biopolymer is swollen by wet ball milling. This may include, but is not limited to, chitin, chitosan, hemicellulose, and pregelatinized corn starch.

[0038] In some embodiments, the biopolymer is swellable with high shear processes other than ball milling. This may include, but is not limited to, microcrystalline cellulose, microfibrillated cellulose, nanocellulose, hairy nanocellulose, konjac glucomannan, hydroxypropyl starch phosphate, high acyl gellan gum, gellan gum, carboxymethyl starch, carboxymethylcellulose (low ds type), agar-agar and agarose.

[0039] It is also conceivable, according to the present invention, to use soluble biopolymers, including, but not limited to, xanthan gum, diuthan gum, sodium alginate and sclerotic gum.

[0040] In some embodiments, the skin care composition according to the invention has a pH between about 6.5 and about 8.5, or about 7 to about 8. In specific embodiments, the skin care composition comprises a chitosan suspension and has a pH between about 7.8 and about 8.1. Petition 870260082686, dated 08 / 14 / 2026, p. 17 / 182 13 / 72

[0041] In some embodiments, the biopolymer molecules or particles that are part of the skincare composition have been mechanically processed into a homogeneous and stable aqueous biopolymer suspension. In some embodiments, the mechanical processing involves high shear and / or high mechanical energy conditions. In some embodiments, the high shear and / or high mechanical energy conditions are obtained by a process that includes, but is not limited to, mechanical shearing, shear grinding, planetary ball milling, rolling mill, vibratory ball mill, rotary agitated ball mill, horizontal media mill, colloidal milling. As indicated below, the high shear and / or high mechanical energy conditions can be maintained for a period, under parameters, under suitable conditions, etc., until a desired change of state is obtained, for example, a change in color, a change in viscosity, a change from a suspension to a paste, ointment, cream, lotion, gel or milk, etc.

[0042] Without intending to limit myself to theory, subjecting the biopolymer to high shear and / or high mechanical energy conditions improves the biopolymer's performance not observed in conventional processes, including enhanced rheological properties such as viscosity, shear thinning properties, and high flow value. Biopolymers dispersed by high shear processes with a fatty alcohol may contain lamellar crystalline gel (LGN) networks that can synergistically increase the viscosity of the biopolymer dispersions. Oil + water biopolymer dispersions, according to the present invention, may also contain Pickering emulsion, in which the water-in-oil or oil-in-water emulsion is stabilized by the biopolymer.

[0043] In some applications, high shear and / or high mechanical energy conditions require the use of a device or Petition 870260082686, dated 08 / 14 / 2026, page 18 / 182 14 / 72 suitable apparatus, including, but not limited to, ball mill (e.g., planetary ball mill, laminating mill, vibratory ball mill, rotary agitated ball mill, horizontal media mill, colloid mill, magnetic mill), twin-screw extruder, high-pressure homogenizer, blade homogenizer, agitated homogenizer, disperser, rotor-stator homogenizer, high-shear mixer, spreader mixer, dynamic mixer, plow mixer, turbine mixer, speed mixer, friction grinder, sonicator (e.g., ultrasonic high-shear processes), tissue shredder, cell lyser, polytron, ribbon agitator, microfluidizer, high-pressure homogenizer and combinations thereof. In preferred embodiments, the present invention utilizes ball milling under wet conditions.Specific examples of ball mills include, but are not limited to, a vertical planetary mill (e.g., Tencan XQM2A™) with 100 mL capacity zirconia jars and 10 mm diameter zirconia spheres, a Flacktek™ speed mixer (DAC 330-11 SE) with a 40 mL zirconia jar with 5 mm diameter zirconia spheres or rings, a 1.5 L Supermill Plus™ with 1.4 to 1.7 mm zirconia spheres, and a Netzsch Labstar with 0.6 to 0.8 mm or 1.4 to 1.7 mm spheres.

[0044] In particular embodiments, biopolymer compositions and suspensions according to the present invention are obtained using a specific protocol herein referred to as the 10+1 Alt method. This method comprises grinding the biopolymer for a specified period of time (e.g., 10 minutes), followed by a brief pause (e.g., 1 minute), and then grinding in the opposite direction for a specified period of time (e.g., 10 minutes) for a total of 1 hour, 2 hours, 3 hours, 5 hours, 10 hours, or 12 hours. Petition 870260082686, dated 08 / 14 / 2026, p. 19 / 182 15 / 72

[0045] Advantageously, the viscosity of the compositions / suspensions can be altered by varying the high shear conditions and / or the mechanical energy to which the biopolymer(s) is / are subjected. These conditions can be adjusted to obtain a stable homogeneous suspension (e.g., a stable homogeneous colloidal suspension) with the desired viscosity. Typically, supplying more mechanical energy will increase the shear and consequently reduce the viscosity of the final product. The biopolymer itself and / or the final drug delivery compositions can be formulated as a paste, an ointment, a cream, a lotion, a gel, or a milk.

[0046] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules or particles are a homogeneous colloidal suspension of biopolymer. In some embodiments, the homogeneous colloidal suspension comprises colloids with a size range of about 1 nm to about 1 μm.

[0047] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise biopolymer fibers. In some embodiments, the fibers have a width of about 1 nm to about 5 μm, or about 5 nm to about 5 μm, about 7 nm to about 5 μm, or about 10 nm to about 5 μm, or about 20 nm to about 5 μm, or about 25 nm to about 5 μm, or about 30 nm to about 5 μm, or about 35 nm to about 5 μm, or about 35 nm to about 3 μm. In some embodiments, the fibers have a width of at least 1 nm, or at least 5 nm, or at least 10 nm, or at least 20 nm, or at least 30 nm, or at least 40 nm, or at least 50 nm, or at least 75 nm, or at least 100 nm, or at least 250 nm, or at least 500 nm, or at least 750 nm, or at least 1 μm, or Petition 870260082686, dated 08 / 14 / 2026, p. 20 / 182 16 / 72 at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or wider.

[0048] In embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise biopolymer fibers with a length of about 1 nm to about 200 μm, about 10 nm to about 100 μm, or about 50 nm to about 10 μm, or about 100 nm to about 10 μm, or about 500 nm to about 10 μm, or about 750 nm to about 10 μm, or about 800 nm to about 10 μm, or about 900 nm to about 5 μm, or about 1 μm to about 10 μm, or about 1 μm to about 5 μm, or about 1 μm to about 3 μm.In some embodiments, the fibers have a length of at least 1 nm, or at least 10 nm, at least 50 nm, or at least 100 nm, or at least 250 nm, or at least 500 nm, or at least 750 nm, or at least 800 nm, or at least about 900 nm, or at least 1 μm, or at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or at least 6 μm, or at least 7 μm, or at least 8 μm, or at least 9 μm, or at least 10 μm, or at least 25 μm, or at least 50 μm, or at least 75 μm, or at least 100 μm, or at least 150 μm, or at least 200 μm or more. In some embodiments, a dry particle size range may be between about 1 nm and about 1 μm, or up to 10 μm, and a wet particle size range may be between about 200 nm and about 20 μm, or up to 200 μm.

[0049] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise biopolymer fibers having: (i) a width greater than 20 nm (for example, at least 25 nm, or at least 40 nm, or at least 50 nm) and a length greater than 50 nm (for example, at least 100 nm, or at least 500 nm, or at least Petition 870260082686, dated 08 / 14 / 2026, p. 21 / 182 17 / 72 μm, or at least 2 μm); or (ii) a width greater than 32 nm (for example, at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 50 nm (for example, at least 100 nm, or at least 500 nm, or at least 1 μm, or at least 2 μm); or (iii) a width greater than 20 nm (for example, at least 25 nm, or at least 40 nm, or at least 50 nm) and a length greater than 500 nm (for example, at least 600 nm, or at least 750 nm, or at least 1 μm, or at least 2 μm); or (iv) a width greater than 30 nm (for example, at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 800 nm (for example, at least 900 nm, or at least 1 μm, or at least 2 μm);or (v) a width greater than 8 nm (for example, at least 10 nm, at least 25 nm, or at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 340 nm (for example, at least 350 nm, or at least 500 nm, at least 750 nm, or at least 900 nm, or at least 1 μm, or at least 2 μm); or (vi) a width greater than 11 nm (for example, at least 15 nm, at least 25 nm, or at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 166 nm (for example, at least 200 nm, or at least 350 nm, or at least 500 nm, at least 750 nm, or at least 900 nm, or at least 1 μm, or at least 2 μm);or (viii) a width greater than 32 nm (for example, at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 800 nm (for example, at least 900 nm, or at least 1 μm, or at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or at least 6 μm, or at least 7 μm, or at least 8 μm, or at least 9 μm, or at least 10 μm, or at least 25 μm, or at least 50 μm, or at least 75 μm, or at least 100 μm, or at least 150 μm, or at least 200 μm or more). Petition 870260082686, dated 08 / 14 / 2026, page 22 / 182 18 / 72

[0050] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise biopolymer fibers in which the average width and average length of the fibers in the composition are as defined above, for example, an average width greater than 20 nm (for example, at least 25 nm, or at least 40 nm, or at least 50 nm) and an average length greater than 50 nm (for example, at least 60 nm, at least 75 nm, or at least 100 nm, or at least 500 nm, at least 750 nm, or at least 1 μm, or at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or at least 6 μm, or at least 7 μm, or at least 8 μm, or at least 9 μm, or at least 10 μm, or at least 25 μm, or at least 50 μm, or at least 75 μm, or at least 100 μm, or at least 150 μm, or at least 200 μm or more).

[0051] In some embodiments, the biopolymer suspensions and / or biopolymer compositions comprising biopolymer molecules have a pH between about 6.5 and about 8.5. In specific embodiments, the biopolymer suspension is a chitosan suspension with a pH between about 7.8 and about 8.1.

[0052] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise biopolymer fibers with a crystalline region and an amorphous region. In some embodiments, the stable homogeneous suspension comprises globular-shaped biopolymer fibers. In some embodiments, the stable homogeneous suspension is composed mainly, or only, of biopolymer nanofibrils in suspension.

[0053] Those skilled in the art are aware that particle size measurements may vary depending on the measurement method and the state of the particles (e.g., particles in Petition 870260082686, dated 08 / 14 / 2026, page 23 / 182 19 / 72 wet state particles are typically larger than the same particles in dry state). Typically, particles will be in a wet or suspended state when measured by dynamic light scattering (DLS) and in a dry state when measured by scanning electron microscopy (SEM).

[0054] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise agglomerated spheres of alginic acid with an average size of about 40 nm to about 80 nm, or about 45 nm to about 75 nm, as measured by scanning electron microscopy (SEM). In some embodiments, the stable homogeneous suspension comprises agglomerated spheres of alginic acid with an average size of about 30 nm to about 70 nm or about 35 nm to about 65 nm, average size of about 40 nm to about 80 nm, or about 45 nm to about 75 nm, as measured by scanning electron microscopy (SEM).

[0055] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise agglomerated cellulose spheres with an average size of about 50 nm to about 80 nm, or about 55 nm to about 75 nm, average size of about 40 nm to about 80 nm, or about 45 nm to about 75 nm, as measured by scanning electron microscopy (SEM). In some embodiments, the stable homogeneous biopolymer suspension comprises agglomerated cellulose spheres with an average size of about 35 nm to about 75 nm or about 40 nm to about 65 nm, average size of about 40 nm to about 80 nm, or about 45 nm to about 75 nm, as measured by scanning electron microscopy (SEM).

[0056] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules Petition 870260082686, dated 08 / 14 / 2026, p. 24 / 182 20 / 72 comprise clustered chitin spheres with an average size of about 45 nm to about 85 nm, or about 50 nm to about 80 nm. In some embodiments, the stable homogeneous biopolymer suspension comprises clustered cellulose spheres with an average size of about 45 nm to about 80 nm or about 50 nm to about 75 nm, as measured by scanning electron microscopy (SEM).

[0057] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise clustered chitosan spheres with an average size of about 75 nm to about 120 nm, or about 80 nm to about 115 nm, or about 85 nm to about 110 nm, as measured by scanning electron microscopy (SEM). In some embodiments, the stable homogeneous suspension comprises clustered chitosan spheres with an average size of about 70 nm to about 100 nm or about 75 nm to about 95 nm, as measured by scanning electron microscopy (SEM).

[0058] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise agglomerated silk spheres with an average size of about 40 nm to about 165 nm, or about 45 nm to about 160 nm, as measured by scanning electron microscopy (SEM). In some embodiments, the stable homogeneous biopolymer suspension comprises agglomerated silk spheres with an average size of about 40 nm to about 150 nm or about 45 nm to about 140 nm, as measured by scanning electron microscopy (SEM).

[0059] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules comprise particles of one or more of the following: alginic acid, cellulose Petition 870260082686, dated 08 / 14 / 2026, p. 25 / 182 21 / 72 se, chitin, chitosan and silk, where the particle size range, measured by SEM, is as defined in the tables and figures of document WO 2022 / 137184.

[0060] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by visual properties similar to those represented in the SEM images shown in the figures of document WO 2022 / 137184.

[0061] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by a Fourier Transform Infrared Spectroscopy (FTIR) spectrum, as represented in the figures of document WO 2022 / 137184.

[0062] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by Solid State Nuclear Magnetic Resonance (SSNMR) characterization, as illustrated in the figures in WO 2022 / 137184.

[0063] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by Power X-ray Diffraction (PXRD) patterns, as illustrated in the figures in document WO 2022 / 137184.

[0064] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by Dynamic Light Scattering (DLS) measurements, such as those reported in WO 2022 / 137184.

[0065] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by a transmittance spectrum, as shown Petition 870260082686, dated 08 / 14 / 2026, p. 26 / 182 22 / 72 in the figures of document WO 2022 / 137184.

[0066] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by a sweep suspension test, as reported in WO 2022 / 137184.

[0067] In some embodiments, the biopolymer and / or biopolymer compositions comprising biopolymer molecules are characterized by rheological behavior, as illustrated in the figure(s) of document WO 2022 / 137184.

[0068] In some embodiments, the biopolymer represents about 0.3% w / w to about 10% w / w of the skin care composition (for example, about 0.3% w / w, or about 0.5% w / w, or about 0.75% w / w, or about 1% w / w, or about 1.5% w / w, or about 2% w / w, or about 2.5% w / w, or about 3% w / w, or about 3.5% w / w, or about 4% w / w, or about 4.5% w / w, or about 5% w / w, or about 5.5% w / w, or about 6% w / w, or about 6.5% w / w, or about 7% w / w, or about 7.5% w / w, or approximately 8% w / w, or approximately 8.5% w / w, or approximately 9% w / w, or approximately 9.5% w / w, or approximately 10% w / w). It is also conceivable, according to the present invention, to achieve higher concentrations of biopolymer in the skin care composition (for example, approximately 11% w / w, approximately 12% w / w, approximately 13% w / w, approximately 14% w / w, approximately 15% w / w).

[0069] In some embodiments, the total amount of biopolymer + water in the skin care composition is close to 100% w / w (for example, about 99.9% w / w, or about 99.5% w / w, or about 99% w / w, about 98.5% w / w, or about 98% w / w, or about 97.5% w / w, or about 97% w / w, or about 96.5% w / w, or about 96% w / w, or about 95.5% w / w, or about 95% w / w). In some embodiments, the total amount of biopolymer + water in Petition 870260082686, dated 08 / 14 / 2026, p. 27 / 182 23 / 72 composition for skin care is equal to or greater than 90% w / w (for example, at least 90% w / w, or at least 91% w / w, or at least 92% w / w, or at least 93% w / w, or at least 94% w / w, or at least 95% w / w, or at least 95.5% w / w, or at least 96% w / w, or at least 96.5% w / w, or at least 97% w / w, or at least 97.5% w / w, or at least 98% w / w, or at least 98.5% w / w, or at least 99% w / w, or at least 99.5% w / w). In some formulations, the total amount of biopolymer + water in the skincare composition is between 90% w / w and 99% w / w, or between 92% w / w and 97% w / w, or between 93% w / w and 96% w / w. Skin care compositions

[0070] A particular aspect of the invention relates to skin care compositions comprising a biopolymer and / or a biopolymer suspension, as described herein. In some embodiments, the biopolymer comprises, or alternatively consists of, a biopolymer composition comprising biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension (for example, a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent).

[0071] In some embodiments, the skin care composition comprises a biopolymer and at least one of: a humectant, an emollient, an occlusive, a ceramide, an emulsifier, a thickening agent, a preservative, an antioxidant, a fragrance, a functional ingredient, a UVB filter, a UVA filter, and a broad-spectrum filter. In some embodiments, the skin care composition comprises at least one biopolymer, an emulsifier / stabilizer, and a preservative.

[0072] In some embodiments, the humectant is selected from the group consisting of glycerin, urea, hyaluronic acid, salicylic acid Petition 870260082686, dated 08 / 14 / 2026, page 28 / 182 24 / 72 co, alpha-hydroxy acid, propylene glycol, honey, sorbitol, butylene glycol, sodium PCA, allantoin, sodium lactate, dicyanamide, tremella extract, seaweed, aloe vera and mixtures thereof. In some embodiments, the humectant represents up to about 20% w / w of the skin care composition (for example, about 0.1% w / w, or about 0.5% w / w, or about 1% w / w, or about 2% w / w, or about 5% w / w, or about 10% w / w, or about 15% w / w, or about 20% w / w).

[0073] In some embodiments, the emollient is selected from the group consisting of beeswax, butylphthalimide, isopropylphthalimide, candelilla oil, castor oil, cocoa butter, coconut oil, cyclomethicone, cyclopentasiloxane, dicaprylyl ether, diisopropyl dimer dilinoleate, dimethicone, dipropylene glycol dibenzoate, glycerin, hydrogenated polyisobutene, isocetyl stearoyl stearate, isododecane, isohexadecane, isoparaffin, isopropyl isostearate, isopropyl palmitate, isopropyl stearate, jojoba oil, lanolin, mineral oil, octyl dodecanol, octyl palmitate, olive oil, palm oil, pentaerythritol tetraoctanoate, petrolatum, phenyl ethyl benzoate, polyglyceryl-4 oleate, propylene glycol, propylene glycol dibenzoate, propylene glycol isostearate, shea butter, sorbitol, squalane, sunflower oil, trisiloxane and mixtures thereof.In some formulations, the emollient represents up to approximately 15% w / w of the composition for skin care (for example, approximately 0.1% w / w, or approximately 0.5% w / w, or approximately 1% w / w, or approximately 2% w / w, or approximately 5% w / w, or approximately 10% w / w, or approximately 15% w / w).

[0074] In some embodiments, the occlusive is selected from the group consisting of mineral oil, petrolatum, lanolin, dimethicone, shea butter and mixtures thereof. In some embodiments, the occlusive represents up to about 10% w / w of the skin care composition (e.g., about 0.1% w / w, or about 0.5%). Petition 870260082686, dated 08 / 14 / 2026, p. 29 / 182 25 / 72 p / p, or about 1% p / p, or about 2% p / p, or about 5% p / p, or about 10% p / p).

[0075] In some embodiments, the ceramide is selected from the group consisting of EOS ceramide, NS ceramide, NG ceramide, NP ceramide, AP ceramide, EOP ceramide, phytosphingosine, sphingosine and mixtures thereof. In some embodiments, ceramides represent up to about 5% w / w of the skin care composition (for example, about 0.1% w / w, or about 0.5% w / w, or about 1% w / w, or about 2% w / w, or about 3% w / w, or about 4% w / w, or about 5% w / w).

[0076] In some embodiments, the emulsifier is selected from the group consisting of glyceryl stearate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, tragacanthins, lecithin, mustard seed, soy lecithin, sodium phosphates, diacetyl tartaric acid ester of monoglyceride, sodium stearoyl lactylate and mixtures thereof. In some embodiments, the emulsifier represents up to about 10% w / w of the skin care composition (for example, about 0.1% w / w, or about 0.2% w / w, or about 0.5% w / w, or about 1% w / w, or about 2% w / w, or about 5% w / w, or about 10% w / w).

[0077] In some embodiments, the thickening agent is selected from the group consisting of aluminum and magnesium, silicate, silica, bentonite, carbomer, cetyl palmitate, ammonium acryloyldimethyltaurate and mixtures thereof. In some embodiments, the thickening agent represents up to about 5% w / w of the skin care composition (for example, about 0.1% w / w, or about 0.5% w / w, or about 1% w / w, or about 2% w / w, or about 3% w / w, or about 4% w / w, or about 5% w / w).

[0078] In some embodiments, the preservative(s) is / are selected^) from: pentylene glycol (e.g., Dermosoft® Pentiol Petition 870260082686, dated 08 / 14 / 2026, p. 30 / 182 26 / 72 Eco), 1,2-Alkanediol (e.g., Hydrolite® 6), phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, DMDM ​​hydantoin, quaternium-15, imidazolidinyl urea, diazolidinyl urea, polyoxymethylene urea, sodium hydroxymethylglycinate, 2-bromo-2-nitropropane-1,3-diol (bromopol), glyoxal, parabens such as ethylparaben, butylparaben, methylparaben, propylparaben, isobutylparaben, isopropylparaben, benzylate, benzoic acid, benzyl ester, methylisothiazolinone (MIT; 2-methyl-4-isothiazolinone-3-one), methylchloroisothiazolinone (CMIT; 5-chloro-2-methyl-4-isothiazolinone-3-one), phenoxyethanol, 2-phenoxyethanol, Neolone 950™, OriStar™ MIT, Microcare™ MT, Methylchloroisothiazolinone (CMIT), phenoxyethanol, 2-phenoxyethanol, Euxyl K™400 (mixture of phenoxyethanol and 1,2-dibromo-2,4-dicyanobutane), triclosan (TSC), triclocarban (TCC), benzyl alcohol, benzalkonium chloride, citric acid, dehydroacetic acid, essential oils, grapefruit seed extract, lactic acid,Levulinic acid, potassium sorbate, sodium dehydroacetate, sodium metabisulfite, sodium salicylate, sorbic acid, vitamin E, zinc pyrithione, Geogard™ ECT, Leucidal™ SF Complete, Leucidal™ Liquid Complete, Leucidal™ SF Max, AMTicide™ Coco, poplar bark powder, NeoDefend™, GeoGard™ Ultra, MicroGuard™, Arboricide OC, Elderberry OS, ascorbic acid, vitamin E, beeswax, honey, essential oils (e.g., lavender, clove, rosemary, tea tree, sage, rosewood, thyme, cinnamon, neem, grapeseed, citrus), grapefruit seed extract (GSE), bentonite clay, copper salts, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), benzoic acid, sodium benzoate, relative benzyl alcohol, propionic acid, propionic salts, salicylic acid and derivatives, sorbic acid, calcium sorbate, potassium sorbate, bromopol, 2-bromo-2-nitropropane-1,3-diol, diazolidinyl urea, DMDM ​​hydantoin, glyoxal, imidazolidinyl urea, polyoxymethylene urea,quaternium-15, sodium hydroxymethylglycinate, zinc pyrithione, sodium lauryl sulfate, Petition 870260082686, dated 08 / 14 / 2026, page 31 / 182, 27 / 72 pylene glycol, caprylyl glycol, AE Prerse PX 82™, blackcurrant powder with phytoside, Lexgard Natural™, Sensiva Go Natural™, Spectrastat™, raspberry ketone, ChemPhyte™, Hydrolite 6™, hexanediol and mixtures thereof. Specific examples include, but are not limited to, 1.5% w / w hexanediol with benzyl alcohol and 0.5% w / w phenoxyethanol. It is within the abilities of those skilled in the art to identify and test suitable preservatives according to the present invention. In some embodiments, the preservative represents up to approximately 5% w / w of the composition for skin care (for example, approximately 0.1% w / w, or approximately 0.2% w / w, or approximately 0.3% w / w, or approximately 0.4% w / w, or approximately 0.5% w / w, or approximately 0.6% w / w, or approximately 0.7% w / w, or approximately 0.8% w / w, or approximately 0.9% w / w, or approximately 1% w / w, or approximately 1.5% w / w, or approximately 2% w / w, or approximately 2.3% w / w, or about 3% w / w, or about 4% w / w, or about 4.5% w / w, or about 5% w / w).

[0079] In some embodiments, the antioxidant is selected from the group consisting of vitamin A, vitamin C, vitamin E, vitamin B3, green tea extract, resveratrol, curcumin, lycopene, coenzyme Q10, polyphenols, C. asiatica extract, tamanu oil, and mixtures thereof. In some embodiments, the antioxidant represents up to about 0.5% w / w of the skin care composition (e.g., about 0.01% w / w, or about 0.05% w / w, or about 0.1% w / w, or about 0.2% w / w, or about 0.3% w / w, or about 0.4% w / w, or about 0.5% w / w).

[0080] In some embodiments, the fragrance is selected from the group consisting of linalool, citronellol, limonene, geraniol, eugenol, lavender oil (Lavandula angustifolia), rose flower extract (Rosa damascene, canina, centifolia, gallica, multiflora), bergamot oil (Citrus bergamia), ylang-ylang oil (Canaga odorata), lemon (Citrus limon), lime (Citrus aurantifolia or Citrus medica), orange (Citrus sinensis). Petition 870260082686, dated 08 / 14 / 2026, p. 32 / 182 28 / 72 sis), tangerine (Citrus tangerine), peppermint (Mentha piperita), spearmint (Mentha spicata), eucalyptus, cinnamon (Cinnamomum), cinnamal and mixtures thereof. In some embodiments, the antioxidant represents up to about 0.2% w / w of the composition for skin care (for example, about 0.01% w / w, or about 0.25% w / w, or about 0.05% w / w, or about 0.1% w / w).

[0081] In some embodiments, the functional ingredient is selected from the group consisting of biological extracts, vitamins, amino acids, peptides, proteins, mycosporines, mycosporine-like amino acids (MAAs), ultraviolet-absorbing imino compounds, such as those described in US patent 9,487,474, essential fatty acids (e.g., alpha-hydroxy acids, beta-hydroxy acids, beta-glucans and mixtures thereof). In some embodiments, the functional ingredient represents up to about 10% w / w of the skin care composition (e.g., about 0.1% w / w, or about 0.5% w / w, or about 1% w / w, or about 2% w / w, or about 5% w / w, or about 10% w / w).

[0082] In some embodiments, the composition may also provide sun protection and further comprises a UVB filter, a UVA filter and / or a broad-spectrum filter that provides a Sun Protection Factor (SPF) of at least 15, or at least 30, or at least 45, at least 50, or at least 60. In some embodiments, the filter (i.e., any one, two or three of the UVB filter, the UVA filter and the broad-spectrum filter) represents up to about 20% w / w of the skin care composition (for example, about 0.1% w / w, or about 0.5% w / w, or about 1% w / w, or about 2% w / w, or about 5% w / w, or about 10% w / w, or about 15% w / w, or about 20% w / w).

[0083] In some applications, the UVB filter is selected from the group consisting of Uvivul™ T 150 (Ethylhexyl Triazone), carbon dioxide Petition 870260082686, dated 08 / 14 / 2026, p. 33 / 182 29 / 72 titanium, benzophenone-3 (oxybenzone), octinoxate (ethylhexyl methoxycinnamate), octocrylene, ethylhexyl salicylate (octisalicylate), homosalate (homomentyl salicylate), ensulizole (phenylbenzimidazole sulfonic acid), amyloxate (isoamyl p-methoxycinnamate), polysilicone-15, diethylhexyl butamido triazone (iscotrizinol), 4-methylbenzylidene camphor (enxacamene) and mixtures thereof.

[0084] In some embodiments, the UVA filter is selected from the group consisting of butyl methoxydibenzoylmethane, butyl methoxydibenzoylmethane (Avobenzone™), diethylamino hydroxybenzoyl hexyl benzoate (Univul™ a plus), terephthalidene dicamphor sulfonic acid (Mexoryl™ SX), disodium phenyl dibenzimidazole tetrasulfonate (Neo Heliopan™ ap), Tinosorb™ S (bis-ethylhexyloxyphenol methoxyphenyl triazine), Tinosorb™ M (methylene bis-benzotriazolyl tetramethylbutylphenol (and) water (and) decyl glucoside (and) propylene glycol (and) xanthan gum), Tinosorb™ A2B (tris-biphenyl triazine (and) water (and) decyl glucoside (and) butylene glycol (and) disodium phosphate (and) xanthan gum) and mixtures thereof.

[0085] In some embodiments, the broad-spectrum filter is selected from the group consisting of bis-ethylhexyloxyphenol methoxyphenyl triazine, tris-biphenyl triazine (nano), methylene bis-benzotriazolyl tetramethylbutylphenol, drometrizole trisiloxane, zinc oxide and mixtures thereof.

[0086] Skin care compositions according to the present invention can find numerous applications, at least to benefit the skin. For example, skin care compositions can provide one or more of the following benefits: skin hydration, improved skin appearance, protection against environmental damage, combating the effects of aging, wrinkle removal and / or sun spot removal.

[0087] In some forms, the skin care composition comprises the following ingredients: Petition 870260082686, dated 08 / 14 / 2026, page 34 / 182 30 / 72 Table 1 - Composition A: FUNCTION(S) % (w / w) IN FORMULATION biopolymer (dry weight) 0.3 - 10 Humectant 0 - 20 Emollient 1 - 15 Occlusive 0 - 10 Ceramide 0 - 5 Emulsifier 0.2 - 10 Thickening agent 0 - 5 Preservative 0.5 - 5 Antioxidant 0 - 0.5 Fragrance 0 - 0.2 Functional ingredient 0 - 10 UVB filter 0 - 20 UVA filter 0 - 20 Broad spectrum filter 0 - 20 Water (to make up 100%) 30-95 Table 2 - Composition B: FUNCTION(S) % (w / w) in the biopolymer formulation (dry weight) 5 - 10 Preservative 0.2 - 5 Water to make 100% 85- 94.8 Table 3 - Composition C: FUNCTION(S) % (w / w) in the biopolymer formulation (dry weight) 5 - 10 Stabilizer 0.2 - 5 Water to make 100% 88 - 94.8 Petition 870260082686, dated 08 / 14 / 2026, page 35 / 182 31 / 72 Table 4 - Composition D: FUNCTION(S) % (w / w) in the biopolymer formulation (dry weight) 5 - 10 Emollient 0.2 - 1 Preservative 0.3 - 5 Stabilizer 0.2 - 1 Water to make 100% 83 - 94.3

[0088] In some modalities with the skin is in accordance with ur es, the composition for care in the following compositions es- Specifics: Table 5 - Composition 3: Ingredient % (w / w) in the formulation: Chitosan (dry weight) ~7.2, Water ~92.4, Sodium benzoate and potassium sorbate ~0.4 Table 6 - Composition 1: Ingredient % (w / w) in the formulation: Chitosan (dry weight) ~7.2, Water ~91.8, Glyceryl stearate ~0.3, Cetyl alcohol ~0.3, Sodium benzoate and potassium sorbate ~0.4 Table 7 - Composition 4: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~7.1 Water ~91.5 Glyceryl stearate ~0.3 Cetyl alcohol ~0.3 Petition 870260082686, dated 08 / 14 / 2026, page 36 / 182 32 / 72 Beeswax ~0.3% sodium benzoate and potassium sorbate ~0.4% Table 8 - Composition 2: Ingredient % (w / w) in the formulation: Chitosan (dry weight) ~7.1, Water ~91.8, Glyceryl stearate ~0.4, Cetyl alcohol ~0.3, Beeswax ~0.7, Sodium benzoate and potassium sorbate ~0.4 Table 9 - Composition 6: Ingredient % (w / w) in the formulation: Chitosan (dry weight) ~7.0, Water ~90.5, Glyceryl stearate ~0.5, Cetyl alcohol ~0.5, Beeswax ~1.1, Sodium benzoate and potassium sorbate ~0.4 Table 10 - Composition 5: Ingredient % (w / w) in the formulation Cellulose (dry weight) 5 Water 95 Table 11 - Composition 7: Ingredient % (w / w) in the formulation Cellulose (dry weight) 10 Water 90 Petition 870260082686, dated 08 / 14 / 2026, page 37 / 182 33 / 72 Table 12 - Composition 8: Ingredient % (w / w) in the formulation: Chitosan (dry weight) ~5.7g, Alpha cellulose ~0.43g, Preservative ~5.0g, Cetyl alcohol ~0.55g, Beeswax ~0.93g, Water to make 100% ~87.4g Table 13 - Composition 9: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~5.0 alpha cellulose ~0.4 Preservative ~5.0 Water to make 100% ~89.6

[0089] The methods of use may involve simple application The composition is applied to the skin. In some embodiments, the composition is applied to areas including, but not limited to, hands, breasts, neck, arms, legs, eye contour, forehead, face, and any other part of the body as needed. The application(s) may be repeated as needed, for example, at different time intervals (e.g., every hour, or every 2 hours, or every 6 hours, or every 12 hours, or once a day, or twice a day, or three times a day, or once, twice, or three times a week, etc.). Although the skin care compositions of the invention are primarily intended for human use, those skilled in the art will readily understand that they may also find many suitable applications in animals. Desirable properties

[0090] Skin care compositions according to Petition 870260082686, dated 08 / 14 / 2026, page 38 / 182 34 / 72 The present invention is preferably stable. Stability means at least that the biopolymer (e.g., fibers, spherical bodies) or any other component of the compositions does not settle to the bottom. In some embodiments, the insoluble and / or semi-soluble biopolymer(s) remain(s) in suspension for at least 1 week, or at least 1 month, or at least 6 months, or at least 12 months, or at least 18 months, or at least two years, or at least three years or more. It is within the competence of those skilled in the art to address any phase separation exhibited by skin care compositions according to the present invention.

[0091] The skin care compositions according to the present invention can be formulated to have a desired viscosity (for example, the viscosity of what is generally referred to as a paste, an ointment, a cream, a lotion, a gel, or a milk). In some embodiments, the viscosity of the compositions / suspensions can be altered by varying the high shear conditions and / or the mechanical energy to which the biopolymer(s) is / are subjected. In some embodiments, the stable homogeneous suspension comprises a viscosity of about 25 mPa to about 85,000 mPa. Table 14 below provides non-limiting examples of desirable viscosity for the compositions / suspensions according to the present invention. Table 14: Examples of desired viscosities Desired Consistency | Viscosity (mPa^s) | Paste | approximately 40,000 to approximately 300,000 | Ointment | approximately 20,000 to approximately 50,000 | Cream | approximately 1,500 to approximately 30,000 | Lotion | approximately 800 to approximately 4,000 | Gel | approximately 1,000 to approximately 40,000 Petition 870260082686, dated 08 / 14 / 2026, p. 39 / 182 35 / 72 Desired Consistency Viscosity Example (mPa^s) Milk approximately 20 to approximately 2000

[0092] In some embodiments, the viscosity of the skin care compositions according to the present invention can be varied by selecting, as needed, the sensory enhancer, film former, emulsifier, emollients, UVB filter, UVA filter and / or broad-spectrum filter.

[0093] The viscosity of the skin care compositions according to the invention can also be varied according to the proportions or concentrations of each of the compounds that make up the composition, in addition to the biopolymer. In some embodiments, the weight ratio of biopolymers:other components (e.g., sensory enhancer, film former, emulsifier, emollients, UVB filter, UVA filter and / or broad-spectrum filter, etc.) is from about 0.1:20 to about 10:20, or from about 0.5:20 to about 3:20, or from about 0.75:20, or from about 1.0:20, 1.25:20, or about 1.5:20. In some embodiments, the skin care compositions according to the invention comprise approximately 0.0110% w / w of biopolymer, or 0.01-5% w / w of biopolymer, or 0.01-2% w / w of biopolymer, or 0.01-1% w / w of biopolymer.In some embodiments, the skin care compositions according to the invention comprise about 0.01% w / w of biopolymer, or about 0.05% w / w of biopolymer, or about 0.1% w / w of biopolymer, or about 0.25% w / w of biopolymer, or about 0.5% w / w of biopolymer, or about 0.75% w / w of biopolymer, or about 1% w / w of biopolymer, or about 1.5% w / w of biopolymer, or about 2.5% w / w of biopolymer, or about 5% w / w of biopolymer.

[0094] The skin care compositions according to the invention may also be formulated as stable emulsions comprising fatty acids (e.g., C10-C22 fatty acids), an oil and / or a wax, and / or comprising N-acetyl glucosamine, Petition 870260082686, dated 08 / 14 / 2026, p. 40 / 182 36 / 72 and / or emulsifiers and preservatives, and / or additives.

[0095] A list of possible additives includes, but is not limited to, preservatives, stabilizers and emulsifiers (e.g., cetyl alcohol, glyceryl stearate, soybean butter, PC90, tara gum, PSC3, PEG, guar gum, xanthan gum, agarose, sodium hyaluronate, Tween 80™, glycerol (humectant)), thickeners, colorants, powders (e.g., mica, pigment, chalk), inks, dyes, fragrances, essential oils, extracts (e.g., plant extract(s) such as aloe vera), vitamins (e.g., ascorbic acid), acids (e.g., acetic acid, citric acid, stearic acid), oils (cocoa butter, emu oil, olive oil, shea butter, silicone oil, mineral oil), metal oxides (e.g., zinc oxides), salts (e.g., sea salts, sodium lactate), honey, clay, propenyl glycol, polyethylene glycol, dry ingredients (e.g., rose petal powder, orange peel powder, chamomile flowers, calendula petals, etc.), allantoin, acetylglucosamine (GlcNAc), waxes (e.g., beeswax), peptides and proteins, pharmaceutical compounds (e.g., N-acetylglucosamine, lidocaine, capsaicin, baclofen, ketamine, methylsulfonylmethane, orphenadrine, tetracaine, amitriptyline, bupivacaine, cyclobenzaprine, doxepin, gabapentin, guaifenesin, acetaminophen, ibuprofen, naproxen, diclofenac, meloxicam, piroxicam, ketoprofen, any NSAIDs), sugars (e.g., glucose, fructose, galactose, etc.), monomers of any cellulose, starch, chitin, chitosan, alginic acid, collagen, silk, etc. The additive(s) may be added before, during, and / or after the high shear and / or high mechanical energy conditions step.

[0096] In some embodiments, the stabilizer is selected from among the following stabilizers: agar, sodium alginate, carrageenan, guar, konjac, tragacanth, locust bean gum, psyllium, tara gum, fenugreek gum, xanthan gum, abietic acid, acetyl maltodextrin. Petition 870260082686, dated 08 / 14 / 2026, page 41 / 182 37 / 72 nosylerithritol, acrylamide / sodium acryloyldimethyltaurate copolymer, acrylates / aminoacrylates / C10-30 alkyl PEG-20 itaconate copolymer, acrylates / C10-30 alkyl acrylate crosspolymer, acrylates / C5-8 alkyl acrylate copolymer, acrylates / stearyl methacrylate copolymer, acrylates / vinyl isodecanoate crosspolymer, acrylates / vinyl neodecanoate crosspolymer, acrylic acid / stearyl acrylate copolymer, acrylic acid / stearyl acrylate / dimethicone methacrylate copolymer, bis-acryloyl poloxamer, alkali polysaccharides, C9-11 alcohols, allyl methacrylate crosspolymer, esters of polyglyceryl-4 of sweet almond oil, aluminum behenate, aluminum caprylate, aluminum dicetyl phosphate, aluminum dilinoleate, aluminum dimyristate, aluminum distearate, aluminum isostearate, aluminum isostearates / laurates / palmitates, aluminum isostearates / laurates / stearatesAluminum isostearates / myristates, aluminum isostearates / palmitates, aluminum lanolate, aluminum monostearate, aluminum myristate, aluminum myristates / palmitates, aluminum / magnesium hydroxide stearate, ammonium acryloyldimethyltaurate / steareth-25 methacrylate crosspolymer, ammonium acryloyldimethyltaurate / steareth-8 methacrylate copolymer, ammonium acryloyldimethyltaurate / vinyl formamide copolymer, ammonium alginate, ammonium rapeseed phosphatidyl, polyacryloyldimethyl ammonium taurate, ammonium shellac, amodimethicone glycerocarbamate, AMP-C8-18 perfluoroalkylethyl phosphate, Aphanothece sacrum polysaccharide, arachidyl alcohol, astragalus gum, root extract of astragalus, avocado DEA, babassu acid, crosslinked bacillus ferment / glucose / sodium glutamate, dextro-laevo-batyl alcohol, hydrolyzed beeswax, synthetic beeswax, bentonite, benzalkonium montmorillonite,Benzalkonium sepiolite, mother liquor emulsion stabilizer, brassica alcohol emollients, Petition 870260082686, dated 08 / 14 / 2026, page 42 / 182 38 / 72 brassicyl isoleucinate esilate, butenediol / vinyl alcohol copolymer, butoxyhydroxypropyl cetyl hydroxyethylcellulose, butter decyl esters, butyl acrylate / isopropylacrylamide / PEG-18 dimethacrylate crosspolymer, butyl babassuate, butylene glycol cocoate, butylene glycol isostearates, C1-5 alkyl galactomannan, C12-13 alcohols, C12-14 sec-pareth-3, C12-14 sec-pareth-5, C12-14 sec-pareth-7, C12-14 sec-pareth-8, C12-14 sec-pareth-9, C12-14 sec-pareth-12, C12-14 sec-pareth-15, C12-14 secpareth-20, C12-14 sec-pareth-30, C12-14 sec-pareth-40, C12-14 secpareth-50, C12-15 alcohols, C12-16 alkyl PEG-2 hydroxypropyl hydroxyethyl ethylcellulose, C12-18 alkyl glucoside, C12-18 hydrogenated triglycerides, C14-15 alcohols, C14-18 glycol, C14-22 alcohols, C15-18 glycol, C16-18 bis-alkyl glyceryl undecyl dimethicone, C18-22 alkyl PEG-25 methacrylate / diethylaminoethyl methacrylate copolymer, C18-30 glycol, C18-38 alkyl hydroxystearoyl stearate,C20-22 alcohols, C20-30 glycol, C20-40 alcohols, C20-40 alkyl crylene, C22-24 pareth-33, C24-28 bis-hydroxyalkyl olivoyl glutamate, C28-52 olefin / undecylenic acid copolymer, C30-50 alcohols, calcium carboxymethylcellulose, calcium carrageenan, calcium laurate, calcium myristate, calcium polyglutamate crosspolymer, calcium potassium carbomer, calcium saccharate, calcium starch octenylsuccinate, calcium stearate, Callitris quadrivalvis resin, candelilla wax, candelilla wax, candelilla / jojoba / rice bran polyglyceryl-3 esters, Cannabis sativa seed oil glycereth-8 esters, caprylyl dimethicone ethoxy glycoside, caprylyl / caprylyl bran / straw glycosides, carbomer 934, carboxymethylcellulose acetate butyrate, carboxymethyl hydroxyethylcellulose, guar carboxymethyl hydroxypropyl, carnauba wax, Carthamus tinctorius oleosomes, hydrogenated castor oil behenyl esters, castor oil phosphate,hydrogenated castor oil stearyl esters, hydrogenated castor oil caprate / caprylate copolymer, Petition 870260082686, dated 08 / 14 / 2026, page 43 / 182 39 / 72 of / sebacic acid, cellulose acetate propionate carboxylate, hydrolyzed cellulose gum, microcrystalline cellulose, ceramide NS / PEG-8 / succinic acid copolymer, ceratonia siliqua gum, ceresin, ceteareth-6 olivate, cetostearyl alcohol, cetyl alcohol, cetyl dimethicone PEG-7 acetate, cetyl dodecenylsuccinate, cetyl hydroxyethyl cellulose, cetyl PEG / PPG-7 / 3 dimethicone, biscetyl PEG-8 cetyl dimethicone, chitosan lauramide succinimide, chitosan lauroyl glycinate, cholesterol / HDI / pullulan copolymer, Citrus aurantium dulcis peel extract, Citrus aurantium sinensis fiber, cocamide, cocamide DEA, cocamide MEA, cocamide MIPA, cocamidopropyl lauryl ether, cocoa butter glyceryl esters, coconut alcohol, coconut oil methylpropanediol esters, hydrolyzed corn starch hydroxyethyl ether, cyamopsis tetragonoloba gum, decyl castorate, decyl glycoside, decyl hemp seedate,7-dehydrocholesterol, dehydroxanthan gum, dicapryl sodium sulfosuccinate, diethylene glycol / dilinoleic acid hydrogen dimer copolymer, digalactosyl glyceryl linoleate / palmitate / oleate, diglycerin / dilinoleic acid / hydroxystearic acid copolymer, dihydrolanosterol, dihydroxyethyl cocamine oxide, dihydroxyethyl lauramine oxide, diisotridecyl lauroyl glutamate, dilauryl maleate / C20 olefin copolymer, dimaltosyl cyclodextrin, dilinoleyl / dimethylcarbonate hydrogen dimer copolymer, dimethicone crosspolymer, dimethicone ethoxy glycoside, dimethicone / lauryl dimethicone / bisvinyldimethicone crosspolymer, dimethicone / PEG-15 dimethyl crosspolymer capramide, dimethyl cocamine, dimethyl lauramine isostearate, dioleyl phosphate, dipropylene glycol isobornyl ether, dodecylhexadecanol, bis-ethoxydiglycol cyclohexane 1,4-dicarboxylate, ethyl hydroxyethyl cellulose, bis-ethyl ppg-behenate dimylate,dimonium methosulfate of bis-(ethyl ppg-3 behenate), ethylene vinyl acetate copolymer, ethylene / acrylic acid copolymer, cup, Petition 870260082686, dated 08 / 14 / 2026, page 44 / 182 40 / 72 Ethylene / Sodium Acrylate Limonene, Feruloyl Soy Glycerides, Perfluorocyclohexylmethanol, Perfluoroheptane, Perfluoromethylcyclohexane, Perfluoromethyldecalin, Gum Ghatti, Glucose Pentaacetate, Alpha-Dextroglucose Pentaacetate, Glycereth-7 Malate, Glycereth-8 Hydroxystearate, Glycereth-7 Benzoate, Hydrogenated Glyceryl Abietate, Glycol Cetearate, Acetylated Glycol Stearate, Glycol Trehalose, Glycereth-8 Grape Seed Oil Esters, Polyglycerin-6 Grape Seed Oil Esters, Hexene / Propylene Maleate Copolymer, Hydroxyquinoline Sulfate, Hydroxyapatite, Hydroxybutyl Methylcellulose, Bis-Hydroxyethoxypropyl Beeswax Esters dimethicone, bis-hydroxyethoxypropyl dimethicone isostearate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, hydroxyethyl cellulose, hydroxyethyl isostearyloxyisopropanolamine, hydroxypropyl cellulose, hydroxypropyl guar gum, hydroxypropyl methyl cellulose,Hydroxypropyl xanthan gum, hydroxypropyltrimonium inulin, hydroxypropyltrimonium xanthan gum, hydroxystearic / linolenic / linoleic polyglycerides, hydroxystearic / linolenic / oleic polyglycerides, inulin lauryl carbamate, synthetic wax from Japan, jojoba oil glycereth-8 esters, hydrogenated lanolin alcohol, lanolin-amide DEA, lauryl alcohol, lauryl alcohol diphosphonic acid, lauryl dodecenylsuccinate, wheat bran / straw lauryl / myristyl glycosides, hydrogenated lemon seed oil, magnesium alginate, maltitol laurate, maltodextrin, PEG-22 methoxy / dodecyl glycol copolymer, PEG-25 methoxy / PPG-25 / 4 dimethicone copolymer, methylcellulose copolymer of methyl vinyl ether-maleic anhydride, montmorillonite, guanidine myristyl / palmitamidobutyl acetate, myristyl alanine, myristyl alcohol, oleic / linoleic / linolenic polyglycerides, olive alcohol, hydrogenated caprylic esters of olive oil,cetyl esters of hydrogenated olive oil, decyl esters of hydrogenated olive oil, hexyl esters of hydrogenated olive oil, lauryl esters of hydrogenated olive oil, myristyl esters of, Petition 870260082686, dated 08 / 14 / 2026, page 45 / 182 41 / 72 hydrogenated olive oil, hydrogenated olive oil stearyl esters, hydrogenated orange seed oil, ozokerite, palm kernel amide DEA, palm kernel amide MEA, palm kernel amide MIPA, palmamide DEA, palmamide MEA, palmamide MIPA, peanutamide MEA, peanutamide MIPA, pectin, tris(peg-2 phenylalanine carboxamide)cyclohexane, peg-2 seboamide DEA, peg-4 peg-12 dimethicone, peg-5 pentaerythrityl dimethylol propionate-2 dendrimer, peg-5 pentaerythrityl dimethylol propionate-3 dendrimer, peg-5 pentaerythrityl dimethylol propionate-4 dendrimer, peg-7 propyl heptyl ether, peg-7m, peg-8 dimethicone / polysorbate 20 crosspolymer, PEG-8 propyl heptyl ether, PEG-9M, PEG-12 carnauba wax, PEG-12 glyceryl linoleate, PEG-14M, PEG-20M, PEG-23M, PEG-65M, PEG-90M, PEG-100 / IPDI copolymer, PEG-114 polylactic acid, PEG-115M, PEG-160M, PEG-180M, PEG-400, PEG-45 / dodecyl glycol copolymer, PEG-450, PEG-500, PEG / PPG-10 / 3 dimethicone oleyl ether, PEG / PPG-100 / 70 tocopheryl etherbis-peg / ppg-15 / 5 dimethicone, isostearato peg / ppg-18 / 18, laurato peg / ppg-18 / 18, tocopheryl ether peg / ppg-2 / 5, dimeticone peg / ppg-20 / 23, bis-peg / ppg-20 / 5 peg / ppg20 / 5 dimethicone, copolymer peg / ppg-2000 / 200, dimethicone peg / ppg23 / 6, tocopheryl ether peg / ppg-30 / 10, tocopheryl ether peg / ppg-5 / 10, tocopheryl ether peg / ppg-5 / 20, tocopheryl ether peg / ppg-5 / 30, tocopheryl ether peg / ppg50 / 20, dimethicone peg / ppg-6 / 4, tocopheryl ether peg / ppg-70 / 30, laurato peg / ppg-8 / 3, alcohol pentadecílico, cera de petrolato microcristalina, acido phosphatidico, phosphatidyl serine, phosphatidylglycerol, pineamidopropyl betaine, polyacrilato de C10-30alquila, crospolymero de polyacrilato-4, crospolymero de polyacrilato-6, crospolymero de polyacrilato-11, polymer cruzado de polyacrilato-14, poliacrilato-10, poliacrilato-11, poliacrilato-27, poliacrilato-28, ácido poliacrílico, polyester-14, polyester-15, polyethyleneo / maleato de isopropyla / copoliol MA, copolymero de poliglyceril-2 diisoestearato / ipdi,sunflower seed crosspolymer of polyglyceryl-3 / citrate, polyglyceryl-4 diisostearate / polyhydroxystearate / sebacate, Petition 870260082686, dated 08 / 14 / 2026, page 46 / 182 42 / 72 polyglyceryl-6 behenate, polypropanediol, polypropylene terephthalate, polyquaternium-2 crosspolymer, polyquaternium-65, polyquaternium-83, polyquaternium-102, polyquaternium-103, polysilicone-25, polyurethane-29, polyvinyl acetate, polyvinylpyrrolidone, potassium alginate, potassium behenoyl hydrolyzed rice protein, potassium behenoyl hydroxyproline, potassium carbomer, potassium carrageenan, potassium stearoyl hydrolyzed rice protein, potassium undecylenoyl alginate, potassium undecylenoyl carrageenan, potassium undecylenoyl hydrolyzed corn protein, potassium undecylenoyl hydrolyzed soy protein, potassium undecylenoyl hydrolyzed wheat protein, hydrolyzed potato tuber extract, alcohol jojoba ppg-4, laureth-2 ppg-4, laureth-5 ppg-6, laureth-3 ppg-20, tocophereth-5 ppg-10, jojoba acid ppg-2, buteth-2 ppg-2, propyl ester of PVM / MA copolymer,Unsaponifiables of Prunus amygdalus dulcis oil, Pseudozyma epicola / Camellia sinensis seed oil / glucose / glycine, soybean meal / malt extract / yeast extract, fermentation filtrate, montmorillonite PVP, PVP / decene copolymer, pyrus malus fiber, sepiolite quaternium-90, rhamnolipids, sclerotia gum, hydrogenated sesame seed oil, sesquiethoxytriethanolamine, sesquioctyldodecyl lauroyl glutamate, shea butter glycerides, silica dimethyl silylate, silica silylate, beta-sitosterol, sodium acrylate / acryloyldimethyltaurate / dimethylacrylamide crosspolymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer, sodium acrylate copolymer sodium acryloyldimethyltaurate / acrylamide, sodium acrylate copolymer / vinyl alcohol, sodium acrylates / vinyl isodecanoate crosspolymer, sodium acryloyldimethyltaurate / acrylamide / VP copolymer,sodium acryloyldimethyltaurate / VP crosspolymer, sodium arachidate, sodium C4-12 olefin copolymer / maleic acid, sodium carbomer, sodium carboxymethylcellulose, carboxymethyl dextran, Petition 870260082686, dated 08 / 14 / 2026, page 47 / 182 43 / 72 sodium, sodium carboxymethyl starch, sodium carrageenan, sodium cellulose sulfate linker, sodium cocoyl barley amino acids, sodium cocoyl / stearoyl (alanine / arginine / asparagine / aspartic acid / glutamic acid / glutamine / glycine / histidine), sodium cyclodextrin sulfate, sodium dextrin octenylsuccinate, sodium laneth sulfate, sodium polyacrylate, sodium polyacrylate starch, sodium polyacryloyldimethyl taurate, sodium polygamma-glutamate, sodium polygamma-glutamate crosspolymer, sodium polyglutamate crosspolymer, sodium polymethacrylate, sodium polynaphthalenesulfonate, sodium polystyrene sulfonate, sodium starch octenylsuccinate, sodium styrene / MA copolymer, sodium tocopheryl phosphate antioxidants, sodium trehalose octenylsuccinate, sodium alginate / TEA-undecylenoyl, sodium carrageenan / TEA-undecylenoyl, sorbitan palmate, soy protein phthalate, soy DEA,Sparassis crispa extract, hydroxypropyltrimonium starch chloride, iso-steareth-200 palmitate, stearic acid, stearyl alcohol, stearyl glycol, stearyl vinyl ether / MA copolymer, Sterculia urens gum, stigmasteryl chloride, stigmasteryl nonanoate, stigmasteryl succinate, styrene / MA copolymer, sucrose polypalmate, sunflower seed oil ethyl ferulate esters, sunflower seed oil polyglyceryl-10 esters, sunflower seed oil polyglyceryl-6 esters, tallow alcohol, tallow amide cosmetic agents, tamarindus indica seed gum, TEA alginate, TEA-dextrin octenylsuccinate, tetradecylicosanoic acid, tetradecyloctadecanoic acid, tetradecyloctadecyl behenate, tetradecyltadecyl myristate, tetradecyltadecyl stearate, tetrasodium etidronate, glyceryl esters of Theobroma grandiflorum seed butter, tocopheryl succinate methylglucamide,Tremella fuciformis polysaccharide, triacontene / VP copolymer, 1-tridecanol, tripropylene glycol, undeceth-40, inulin undecylenoyl gum, Petition 870260082686, dated 08 / 14 / 2026, page 48 / 182 44 / 72 undecylenoyl xanthan gum, vinyl alcohol / vinylformamide copolymer, bis-vinyldimethicone / dimethicone copolymer, bisvinyldimethicone / PEG-10 dimethicone crosspolymer, hydrotreated hydrogenated microcrystalline wax, wellan gum, xanthan gum, zinc undecylenoyl hydrolyzed wheat protein.

[0097] The skin care compositions according to the invention may also comprise microbial ingredients (e.g., filtrates, extracts and / or ferments of Saccharomyces, Lactobacillus) and / or microorganism-based formulations, such as Lallhair® P&R from Lallemand, Lallbiome™ 300, Lallbiome™ 201, Lallskin® Hydra and / or Lallskin® Zn.

[0098] The skin care compositions according to the invention may also comprise cross-linked hyaluronic acid. This compound can be used, for example, as a thickener to produce a transparent moisturizing hydrogel. Specific examples include, but are not limited to, Hyacross™ TG300 Hyaluronic Acid Microspheres (sodium hyaluronate crosspolymer, Bloomage Biotechnology Corp. Ltd.) and Hylasome™ EG10 (sodium hyaluronate crosspolymer, Vantage Personal Care).

[0099] In some embodiments, the skin care composition according to the invention provides at least one of the following skin-related benefits: i. to soften the appearance and / or reduce the presence of fine lines and / or wrinkles; ii. improve the appearance of under-eye bags and facial wrinkles; iii. protect the skin (e.g., from the environment, such as sun, chemicals, etc.; protection against oxidative stress); iv. lighten age spots (e.g., provide lightening of brown age spots to a lighter tone) Petition 870260082686, dated 08 / 14 / 2026, page 49 / 182 45 / 72 more even skin tone); v. to mattify the skin (i.e., to make the skin less shiny); vi. to regenerate and / or rejuvenate the skin; vii. Repair and / or heal the skin (e.g., wound healing); viii. to thicken the skin and / or improve one or more mechanical properties of the skin (firmness, tone, elasticity and / or flexibility); ix. improve skin elasticity; x. create a protective film on the skin, for example, a long-lasting protective film, lasting at least 24 hours, or at least one week, or at least ten (10) days; xi. to create on the skin a film that is almost dry to the touch (for example, a perceptible, but very slight, film-like sensation on the skin's surface); xii. create a refreshing sensation on the skin during application; xiii. to hydrate and / or moisturize the skin; xiv. prevent water evaporation from the skin; xv. improve the integrity of the skin barrier; xvi. reduce epidermal water loss and / or improve water retention by the skin; and xvii. non-comedogenic.

[00100] In some embodiments, the skin care composition according to the invention consists of an instant skin tightener. In some embodiments, the skin care composition provides immediately noticeable benefits to the appearance of the skin. For example, the skin care composition according to the invention preferably dramatically improves the appearance of under-eye bags and facial wrinkles.

[00101] In some modalities, a tensing composition of the pe Petition 870260082686, dated 08 / 14 / 2026, p. 50 / 182 46 / 72 le, according to the present invention, comprises biopolymer molecules, as defined herein, in combination with any suitable skin-tightening compound. For example, a skin-tightening composition, according to the present invention, may comprise one or more of the following: sodium silicate; polyol(s), such as glycerin, propylene glycol and butylene glycol; clay(s), such as bentonite, aluminum magnesium silicate, sodium lithium silicate and magnesium sodium fluorosilicate; soft-focus powder(s), such as spherical silica, cellulose, polymethylsilsesquioxane, dimethicone / vinyldimethicone crosspolymer; dimethylacrylamide / acrylic acid / ethyl methacrylate copolymer and polyurethane-2, polymethyl methacrylate; polysaccharide(s) such as xanthan gum; resin(s) such as sodium polystyrene sulfonate;Natural-based polymer(s) such as pullulan, egg albumin, gliadin, acacia gum, rhizobia gum, hydrolyzed wheat protein / PVP, hydrolyzed Chenepodium quinoa seed, hydrolyzed sesame protein PG and porphyridium-frutaron polysaccharide; synthetic polymer(s) such as sodium polystyrene sulfonate, polyacrylate 21, adipic acid / neopentyl glycol crosspolymer, hdi / trimethylol hexylactone crosspolymer; humectant(s).

[00102] A skin-tightening composition according to the present invention may also comprise biopolymer molecules, as defined herein, and be mixed and / or used in combination with a two-part formulation that is activated when the two parts are combined. This includes, for example, products comprising a prepolymer and an activating catalyst that promotes the crosslinking or polymerization of the polymer. A specific example includes Neotensil™. Consequently, the present invention encompasses skin care kits comprising two-part formulations that are activated when combined, wherein at least one of the formulations comprises biopolymer molecules, as defined herein. Petition 870260082686, dated 08 / 14 / 2026, page 51 / 182 47 / 72

[00103] The skin care composition according to the invention may also find uses in other skin-related applications, including, but not limited to, wound healing, regulation of excess sebum, prevention and / or treatment of acne, antimicrobial activity, providing beneficial antioxidants and / or scavengers to the skin, as well as many other useful film-forming protective activities.

[00104] It is within the competence of those skilled in the art to evaluate any compositions formulated for skin care, according to the present invention, that balance performance and irritation potential with good appearance, feel, and comfort of the product on the skin. Those skilled in the art may also formulate skin care compositions that meet key desirable performance parameters such as film durability over time, resistance to cracking, peeling, whitening, flaking, minimization of skin shine, ease of use, uniform and easy application of the product, etc.

[00105] It is also within the competence of those skilled in the art to evaluate any potentially useful applications of the skin care compositions according to the present invention. This may involve, for example, conducting trials with multiple patients and / or using specialized equipment or tests to measure skin conditions (e.g., hydration, color, softness, thickness, pH, transepidermal water loss (TEWL) measurement, comedogenicity and irritation, etc.). Manufacturing and replacement methods for microplastics

[00106] Another aspect of the present invention relates to methods of manufacturing skin care compositions, as defined herein. A related aspect concerns the use of a biopolymer, as described herein, to replace microplastics in Petition 870260082686, dated 08 / 14 / 2026, page 52 / 182 48 / 72 compositions for skin care.

[00107] In one embodiment, the method comprises replacing at least a portion of microplastics that may be present in skin care compositions with a biopolymer, for example, a biopolymer suspension, as defined herein. In embodiments, the biopolymer suspension comprises a suspension of insoluble and / or semi-solid particles stably dispersed in a polar solvent.

[00108] In one embodiment, the method for manufacturing the skin care composition and / or the method for replacing microplastics in a skin care composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; (2) mixing the biopolymer suspension with at least one of the following: humectant, emollient, occlusive, ceramide, emulsifier, thickener, preservative, antioxidant, fragrance, functional ingredient, UVB filter, UVA filter, and broad-spectrum filter. Clearly, the skin care composition according to the invention is formulated (e.g., ingredient selection, ratio, etc.) to obtain a skin care composition that is dermatologically safe for repeated applications to human skin.

[00109] Those skilled in the art will recognize, or be able to determine, using only routine experimentation, numerous equivalents to the specific procedures, embodiments, claims, and examples described herein. Such equivalents are considered to be within the scope of this invention and covered by the appended claims. The invention is further illustrated by the following examples, which should not be construed as further or specific limitations. Petition 870260082686, dated 08 / 14 / 2026, page 53 / 182 49 / 72 EXAMPLES Example 1: Manufacturing seven (7) different formulations for skin care.

[00110] Seven different biopolymer formulations (referred to here as samples 1 to 7) were manufactured with the aim of evaluating their skin care properties. In samples 1 to 4 and 6, the biopolymer was chitosan. In samples 5 and 7, the biopolymer was cellulose. Sample #3 CMCB3.1

[00111] The fungal chitosan was processed using a two-step process, as follows.

[00112] Step 1: Chitosan was milled for 150 minutes in isopropanol using a CMC BM-100™ basket mill to reduce particle size. The overall milling conditions were 1600 feet per minute (FPM) milling shaft speed and 15 Hz mixing shaft speed, with a 75% loading of 1.4 to 1.7 mm zirconia beads, where 1 kg of powder was processed in isopropanol with 20% w / w solids to obtain a flowable paste. The flowable paste was then dried to remove any isopropanol and obtain a refined powder. This refined powder was used in the next step.

[00113] Step 2: Dry chitosan (785 g) was milled with 13 kg of distilled water for 150 minutes using a 1.5 L Supermill Plus™ (continuous flow horizontal mill) to produce a suspension referred to here as CMCB3.1 chitosan suspension. The general milling conditions for obtaining this suspension were a rotation speed of 2400 FPM (feet per minute) with a pump flow rate of 5.5 GPH (gallons per hour), using 1.1 L of 1.25 mm zirconia beads, where 14 kg of fluid paste were processed with a solids content of 7.18% w / w.

[00114] Sample #1 2022111602 Petition 870260082686, dated 08 / 14 / 2026, page 54 / 182 50 / 72

[00115] A chitosan suspension with fatty alcohol was generated by the combined grinding of chitosan suspension CMCB3.1 (30.1 g), glyceryl stearate (0.104 g) and cetyl alcohol (0.102 g) at 670 RPM with fifty 10 mm sphere units, using the 10+1 Alt method, being ground for ten minutes, followed by a one-minute pause and then ground for ten minutes in the opposite direction, totaling 1 hour. Sample #4 2022111603

[00116] A chitosan suspension with fatty alcohol and beeswax was generated by the combined grinding of chitosan CMCB3.1 (30.1 g), glyceryl stearate (0.100 g), cetyl alcohol (0.103 g) and beeswax (0.1 g) at 670 RPM with fifty 10 mm spheres, using the 10+1 Alt method, being ground for ten minutes, followed by a one-minute pause and then ground for ten minutes in the opposite direction, totaling 1 hour. Sample #2 2022111604

[00117] A chitosan suspension with fatty alcohol and beeswax was generated by grinding the chitosan suspension CMCB3.1 (30.0 g), glyceryl stearate (0.109 g), cetyl alcohol (0.103 g) and beeswax (0.2 g) at 670 RPM with fifty 10 mm sphere units, using the 10+1 Alt method, being ground for ten minutes, followed by a one minute pause and then ground for ten minutes in the opposite direction, totaling 1 hour. Sample # 6 2022112103

[00118] A chitosan suspension with fatty alcohol and beeswax was generated by joint grinding the chitosan suspension CMCB3.1 (30.0 g), glyceryl stearate (0.154 g), cetyl alcohol (0.145 g) at 670 RPM with fifty 10 mm sphere units, using the 10+1 Alt method. Grinding was performed for ten minutes, followed by a one-minute pause, and then grinding for ten minutes. Petition 870260082686, dated 08 / 14 / 2026, p. 55 / 182 51 / 72 minutes in the opposite direction, totaling 1 hour. Then, beeswax (0.345 g) was added and grinding continued at 670 RPM with fifty 10 mm spheres, using the 10+1 Alt method. Sample # 5 CMC B1.C

[00119] Alphacellulose (Sigma Aldrich) was milled in distilled water using a 1.5 L Supermill Plus™. The general milling conditions were at a rotational speed of 2400 FPM with a pump flow rate of 7.3 GPH, using 982 mL of 1.4 to 1.7 mm zirconia beads, where 10 kg of suspension were processed at a solids content of 5% w / w (0.5 kg cellulose and 9.5 kg water). The cellulose was suspended during the milling process for 140 minutes, producing a suspension. Sample #7 CMC B2C

[00120] The cellulose was processed using a two-stage process.

[00121] Step 1: Alpha cellulose (Sigma Aldrich) was milled for 23 hours in isopropanol using a 1.5 L Supermill Plus™ to reduce particle size. The general milling conditions were at a rotation speed of 2750 FPM with a pump flow rate of 10 GPH, using 982 mL of 1.25 mm zirconia beads, where 10 kg of pulp were processed with a solids content of 10% w / w (1 kg of cellulose and 9 kg of isopropanol). The pulp was then dried to remove any isopropanol and obtain a refined powder, which was then used in the next step.

[00122] Step 2: 1 kg of dry cellulose from step 1 was milled in 9 kg of distilled water for 435 minutes using the 1.5 L Supermill Plus™ to produce a suspension. The general milling conditions were a rotation speed of 2400 FPM with a flow rate of 11 GPH, using 956 mL of 1.25 mm zirconia beads, where 10 Petition 870260082686, dated 08 / 14 / 2026, page 56 / 182 52 / 72 kilograms of fluid paste were processed with a solids content of 10% w / w. Example 2: Testing the cosmetic properties of the seven different formulations from Example 1. Summary

[00123] A home self-assessment test was conducted to determine the cosmetic properties of seven (7) different cream formulations manufactured in Example 1. The assessment was based on a woman’s visual and sensory self-assessment. The woman did not apply any moisturizing product to her skin 72 hours before the test and did not use any other product during the test. Her hands were washed, as on any normal day, at least 2 times a day with neutral soap. Her hands were also exposed to water and soap during her daily shower. Test details

[00124] The objective of the test was to evaluate hydration, wrinkle disappearance (film-forming effect) and sensory sensation during application of cream samples to the skin. The test was conducted over nine (9) days, using the following experimental parameters: Table 15 Number of participants Age Sex Ambient temperature Application time # Application in 24 hours Test site Skin type Type applied by use Application type 1 55 F 19.5 oC morning and night 2 back of dry hand 0.3 g superficial

[00125] A predetermined amount of each cream sample was applied to the back of the hand with a stainless steel spatula, following the application scheme illustrated in Figure 1. After transferring the cream to the skin with the spatula, it was spread with the Petition 870260082686, dated 08 / 14 / 2026, page 57 / 182 53 / 72 finger, performing back and forth movements on the spot, from top to bottom, 10 times.

[00126] Sensory properties were also evaluated by rubbing a small amount of cream between the thumb and index fingers to assess the absorption rate, pilling, and texture of each sample. Visual and sensory observation of the tested samples

[00127] The visual and sensory observations of the seven samples tested are summarized in Table 16 below. Images of the samples at time T0 are shown in Figure 2. Petition 870260082686, dated 08 / 14 / 2026, page 58 / 182 54 / 72 Table 16: Visual and sensory observations of the seven samples tested. Sample Application Visual observation of the sample in the bottle Color Texture Separation Finger friction test Immediate observation after application Sample #1: 2022-111602 Smooth application, very fast absorption and no pilling pleasant and shiny texture light beige soft, very slightly watery, slight medicinal odor the bottle is full but there seems to be some separation around the bottle cap very light and slippery feel, oily / silicone feel, the skin seems to absorb a large amount, gritty residue on the skin that seems to melt into the skin after a while, very slight moisturizing sensation. Moisturizing sensation after 30 minutes on the finger Sample #2: 2022-111604 application is difficult, absorption is slow and there is a lot of pilling immediately, but it is still able to penetrate the skin.Light / medium beige, bright texture between a gel and a cream, feels more consistent in the middle + medicinal odor, light separation occurring at the top, smoother feel, slower absorption sensation; more like a cream, the skin seems to absorb a larger amount, no residue on the skin, the skin feels full, good feeling of hydration after 30 minutes. Petition 870260082686, dated 08 / 14 / 2026, page 59 / 182 55 / 72 Sample Application Visual observation of the sample in the bottle Color Texture Separation Finger friction test Immediate observation after application Water under a layer, cool sensation after application, fingers even appear visually more voluminous after application. Sensation of greater hydration after application Sample #3: CMC B3.1: Smooth application, light white deposit on the skin, rapid absorption, observe separation after a few days, seems to be more each day Medium beige Very watery, slight odor High separation in the jar Low absorption rate, pilling formation at the end, seems to stay on the skin surface and is not absorbed, but dries during application, pilling formation at the end, smooth finish on the skin. Large formation of hair on the skin after application.Sample #4: 2022-111603 Smooth application, fast absorption, no pilling, dries easily, shiny, texture seems a little creamier, light beige, good consistency, with slight irregularities in the bottle, very slight separation, observation of a small crust on the side of the bottle and never seems to penetrate, feeling of cold during application, a slight gritty sensation followed by a feeling of hydration after 30 minutes of application. Petition 870260082686, dated 08 / 14 / 2026, page 60 / 182 56 / 72 Sample Application Visual observation of the sample in the bottle Color Texture Separation Finger friction test Immediate observation after application cap, seems to dry quickly smoother sensation to finish with a feeling of dryness and heavy pilling Sample #5: CMC B1.C Smooth application, very fast absorption, a little pilling very shiny gelatinous texture Almost white shiny texture, light cream, more visually interesting texture without visual separation, very subtle smell Application in three (3) steps, initially seems watery and non-slip, then you feel a smooth and slippery effect and finish with a dry touch sensation. A little cold, absorbs very quickly at the beginning, forming clumps at the end. Refreshing effect. The application sensation is good. Sample #6: 2022-112103 Smooth application, forms a little pilling, leaves skin soft and moisturized.More creamy upon application. Shiny, light beige, watery texture. Visually creamy, a medium separation at the top of the bottle seems to absorb slowly, leaves a dry and unpleasant slippery skin surface after application, noticeable cold sensation, very little buildup. Petition 870260082686, dated 08 / 14 / 2026, page 61 / 182 57 / 72 Sample Application Visual observation of the sample in the bottle Color Texture Separation Finger friction test Immediate observation after application Pilling Sample #7: CMC B2C Smoother application, very fast absorption, very light pilling, shiny almost creamy texture, much lighter color, light gray / white, dries very quickly on the skin, different smells, less medication, some slight separation at the top of the bottle. Feels watery at first, but changes to a more moisturizing and smooth feel, with a good application sensation. Forms pilling at the end when it starts to dry. The skin feels denser and almost fleshier. Petition 870260082686, dated 08 / 14 / 2026, page 62 / 182 58 / 72 Quantitative Evaluation of Cream Samples

[00128] The samples were evaluated during the nine days of the test and, each day, received a score from 1 to 10 in various visual and sensory aspects. The observations measured for samples 1 to 3 are illustrated and summarized in Figure 3. The observations measured for samples 4 to 6 are illustrated and summarized in Figure 4. Results and discussion

[00129] The aim of the test was to verify whether any and / or all of the cream samples had film-forming properties and whether these samples could soften the appearance of fine lines and wrinkles on the back of the hand. The aim of the test was also to evaluate the amount of product needed to obtain visual results and how long the results could last.

[00130] Based on the individual's self-assessment, all samples demonstrated film-forming properties. All samples also demonstrated the ability to smooth the skin surface, leaving it very soft to the touch after application of the creams.

[00131] A rather unusual and interesting effect was observed with the tested creams, compared to traditional skin products available on the market. When applied to the skin, most skin products leave a moist / sticky and shiny feel and touch. In contrast, the samples tested in this test left a perceptible sensation of a very light film on the skin's surface. This film was almost dry to the touch. The skin's surface did not appear moist or hydrated, and was completely opaque. However, the skin under this thin protective film was visually softer, denser, almost plumped, and much more supple. Fine lines and wrinkles were less visible and even more noticeable. Petition 870260082686, dated 08 / 14 / 2026, page 63 / 182 59 / 72 feared they had been repaired.

[00132] Sample #2 (2022-111604) appears to be an excellent candidate for smoothing the appearance of not only fine lines but also wrinkles. Samples #1 (2022-11602) and #4 (2022-111603) were also great candidates.

[00133] Sample #4 (2022-111603) and sample #5 (CMC-B1C) appeared creamier during application, but produced some pellet formation due to intense friction during application. The absorption rate was also very fast. Pellet formation was also observed in sample #3 (CMC-B3.1) when applied to a larger area.

[00134] Sample #7 (CMC-B2C) demonstrated some very special properties: during application and for a few minutes afterwards, a cooling sensation was easily observed. There was also a rapid increase in skin density. Such properties may be extremely interesting in combating age-related damage and treating density loss in areas such as the neck, hands and eye contour.

[00135] A subtle, non-unpleasant medicinal smell was observed in most samples. This smell disappeared quickly during application and no residual odor was observed after application. This subtle odor may be a good way to differentiate the skin products of the invention from commercially available products, since the latter usually contain strong perfume odors that are often not appreciated by consumers.

[00136] Most samples, except #5 (CMC-B1C), showed different degrees of phase separation, with samples #4 (2022-111603) and #7 (CMC-B2C) showing a lower degree.

[00137] It was also observed that, once the films are Petition 870260082686, dated 08 / 14 / 2026, page 64 / 182 60 / 72 caraam on the skin, they were rather persistent, as they were not removed with simple rinsing or mild soap. The protective films lasted more than 24 hours. The presence of such a film likely contributes to maintaining the skin's natural hydration, for example, by preventing water evaporation.

[00138] In addition, a brightening effect was observed in at least 3 of the samples (#2, #4 and #5). After application of these samples, the skin showed a more even tone and existing brown age spots appeared much lighter (see Figures 3 and 4). Example 3: Manufacturing two (2) additional formulations for skin care.

[00139] Four (4) additional biopolymer formulations (referred to herein as samples #8, #9, #10 and #11) were manufactured with the aim of evaluating their skin care properties. Sample # 8 - Composition based on cellulose / chitosan / beeswax [Batch DEF23051502]

[00140] The cellulose / chitosan / beeswax-based composition contained 5.7% w / w mushroom chitosan, 0.43% w / w alpha cellulose, 5% w / w Dermosoft™ pentiol ecoby (Evonik), 0.55% cetyl alcohol and 0.93% beeswax [Batch DEF23051502].

[00141] The composition was prepared as follows. First, an original batch of milled formula containing alpha cellulose, chitosan, and water was milled in a NETZSCH LabStar Zeta Small Media Mill™ for 110 minutes (PSI 6.9 to 11, stirrer speed 3000 rpm, chiller on to maintain a temperature of 32 °C). Separately, the following oil mixture was made by melting cetyl alcohol, synthetic beeswax pellets, and Dermosoft™ at 57 °C. The oil mixture was stirred until the temperature reduced to 52 °C. The milled formula (total of 385 grams) was added Petition 870260082686, dated 08 / 14 / 2026, page 65 / 182 61 / 72 added 1 / 6 at a time to the oily mixture. Both were mixed until the temperature dropped to 45 °C. Once homogeneously mixed, this resulting mixture was added very slowly to the ball mill with the remainder of the original batch of formula ground at the 110-minute mark. Grinding was then continued for another 10 minutes. Sample # 9 - ChitoCellDF™ [DEF23041801]

[00142] A cellulose / chitosan-based composition, according to the invention, comprising 5% w / w mushroom chitosan, 0.4% w / w cellulose, 5% w / w Dermosoft™ and water, was prepared [Batch DEF23041801]. Briefly, the chitosan and cellulose were wet-ground with water for 30 minutes (stirrer pressure 7 to 9 PSI; stirrer speed 3000 rpm). The Dermosoft™ preservative was added at 30 minutes and grinding continued for a further 60 minutes. The temperature of the mixture was maintained between approximately 30 °C and 40 °C. Sample # 10 - ChitoCellDF™ [DEF23110702]

[00143] A cellulose / chitosan-based composition, according to the invention, was prepared like the other samples above. The composition contained water, 5% w / w mushroom extract, 5% w / w Dermasoft™ extract, 3% w / w vegetable glycerin extract, 2.5% w / w beeswax extract, 2% w / w cetyl alcohol extract, 2% w / w adiphylline extract, 1.5% w / w sepilift extract, 0.65% w / w rosehip oil extract, 0.4% w / w cellulose extract (INCI: water, chitosan extract, pentylene glycol, beeswax extract, glycerin extract, cetyl alcohol extract, acetyl hexapeptide 38, dipalmitoyl hydroxyproline extract, Rosa moschata seed silane extract, cellulose). Sample # 11 - ChitoCellDF™ [DEF23110901]

[00144] A cellulose / chitosan-based composition, according to the invention, was prepared like the other samples above. The composition contained water, chitosan 5% w / w, Dermosoft 5% w / w, oil Petition 870260082686, dated 08 / 14 / 2026, page 66 / 182 62 / 72 comes in 1000 4% w / w, argireline 4% w / w, jojoba butter 3% w / w, beeswax 2.5% w / w, cetyl alcohol 1% w / w (INCI: chitosan, beeswax, cetyl alcohol, pentylene glycol, Simmondsia chinensis oil, hydrogenated vegetable oil, tocopherol, acetyl hexapeptide-8, sodium benzoate, cetostearyl olivate, sorbitan olivate). Example 4: Evaluating effectiveness on wrinkles and fine lines Summary

[00145] The cellulose / chitosan-based composition identified as Sample #9 in Example 3 (i.e., batch DEF23041801) was tested in vivo to evaluate efficacy in treating wrinkles and fine lines in 11 healthy male and female volunteers aged 18 to 65 years. The composition was evaluated using standard guidelines by an independent laboratory. Test details

[00146] This study aimed to evaluate the effectiveness of the product in improving wrinkles and fine lines using the VisioTOP-300™ with the AEVA-V4™ software system (EOTECH, France) under normal conditions of use after 15 minutes of the first application and using the product twice a day, daily, for a period of 10 days ± 2 days. 3D images were used to monitor the effects of the product with the VisioTOP-300™ before product use (T0), after 15 minutes of the first application (T15 min), as well as after 10 days of product use (T10 Days ± 2 days).

[00147] The product was used after the first measurement (T0), on the volar surface of the face, twice a day, daily, for a period of 10 days (T10 Days ± 2 days).

[00148] Measurements were taken using the instrument at each experimental time point (T0, T 15Min, and T10 Days ± 2 days) at the level of the treated area. The skin points analyzed at different times were taken on a randomized side of the face for each analysis time point. A Petition 870260082686, dated 08 / 14 / 2026, page 67 / 182 Area 63 / 72 was evaluated in each macro photograph using the VisioTOP300™.

[00149] The distribution of values ​​obtained during measurements at different experimental times for the area treated with the product was compared with an analysis (T15 Min versus T0 and T10 Days ± 2 days versus T0) using Student's t-test. Values ​​of p < 0.05 were considered significant.

[00150] Crow's feet assessment was used to evaluate the product's effectiveness. The most representative parameters for evaluating anti-wrinkle efficacy, namely Ra and Rz, were also measured by the instrument, where Ra is the average linear roughness of the profile and Rz is the average maximum difference in profile height. Results and Conclusions

[00151] The product was highly effective in improving roughness and crow's feet wrinkles. In fact, the study observed a statistically significant reduction in wrinkles after application of the tested composition.

[00152] In summary, the average Rz parameter (Maximum Average Difference in Profile Height) showed a significant decrease of -5.3% on average after 15 minutes of product application (T15 Min) and a significant decrease of -7.5% on average after 10 days of product use (T10 Days ± 2 days), respecting the average value measured at T0 (before product application). These results are shown in Table 17 and Table 18. Table 17. Average Rz in the Crow's Feet Zone Time Average values ​​T0 0.0381 T15Min 0.0361 Petition 870260082686, dated 08 / 14 / 2026, page 68 / 182 64 / 72 Time Average values ​​T10 Days ± 2 days 0.0353 Table 18. Mean % variation of Rz and p-value of Student's t-test. AVERAGE TIME % variation p-value T15 Min vs T0 -5.3% 0.0039 T10 Days ± 2 days vs T0 -7.5% 0.0020

[00153] In particular, the average Ra parameter (Average Linear Profile Roughness) showed a significant reduction of -2.6% on average after 15 minutes of product application (T15 Min) and demonstrated a significant reduction of -3.1% on average after 10 days of product use (T10 Days ± 2 days), compared to the average value measured at T0 (before product application). The average Ra values ​​in the Crow's Feet zone are shown in Table 19. Table 19. Average Ra in the Crow's Feet Zone Time Average values ​​T0 0.0122 T15 Min 0.0119 T10 Days ± 2 days 0.0118

[00154] Considering that the P values ​​refer to a statistically significant variation in the Ra value when below 0.05 (p-value < 0.05), the variation can be considered statistically significant, according to the analysis of the p-value in the comparison of T15 Min with T0 (0.0291). The variation can be considered statistically significant, according to the analysis of the p-value in the comparison of T10 Days + 2 days with T0 (0.0179). The mean % variation of the mean Ra and the p-value of the Student's t-test are shown. Petition 870260082686, dated 08 / 14 / 2026, page 69 / 182 65 / 72 in Table 20. Table 20. Average % variation of the mean Ra and p-value of the Student's t-test. TIME Average % change p-value T15 Min vs T0 -2.6% 0.0291 T10 Days ± 2 days vs T0 -3.1% 0.0179

[00155] Consequently, these results clearly demonstrate that the biopolymeric compositions according to the present invention are active and useful in reducing wrinkles and fine lines. Example 5: Evaluation of Skin Barrier Effect and Water Resistance - Cellulose / Chitosan Based Composition

[00156] The objective of this study was to demonstrate the skin barrier effect and waterproofing effect of a cellulose / chitosan-based composition according to the invention. The cellulose / chitosan-based composition is identified as Sample #9 in Example 3 (i.e., batch DEF23041801). The study involved 13 volunteers and the evaluation was performed by two specialized technicians. Skin barrier effect

[00157] Under the experimental conditions adopted, the product under study demonstrated a significant decrease in Transepidermal Water Loss (TEWL) values ​​in the skin where the product was applied. The product showed a variation of -10.1% after T8 hours ± 2 hours and -16.4% after T12 hours ± 2 hours (see Table 21). Both values ​​were statistically significant when compared to T0. Table 21: Variation of TEWL values Percentage variation of average TEWL values ​​TIME Batch DEF23041801 Control T8 Hours ± 2 hours -10.1% 1% Petition 870260082686, dated 08 / 14 / 2026, pp. 70 / 182 66 / 72 Percentage variation of average TEWL values ​​Batch TIME DEF23041801 Control T12 Hours ± 2 hours -16.4% 0.8%

[00158] Consequently, these results clearly demonstrate that the biopolymeric compositions according to the present invention have positive effects on skin barrier integrity and are effective in reducing epidermal water loss and / or improving water retention by the skin. Waterproof effect

[00159] To evaluate the waterproof effect, a base pigment called Timica® Terra Brown was used for comparison with the cellulose-based composition under study. The evaluation process included splashing water on the forearms of volunteers after application of the tested product (i.e., cellulose / chitosan-based composition, as defined above). During the evaluation, two technicians assigned scores ranging from 1 to 10 based on the persistence of the tested product after 10 splashes of water. If the average of these scores is equal to or greater than 7, the product is considered waterproof after 10 splashes of water from the initial application.

[00160] The two expert technicians gave the tested product an average score of 9.1 out of 10 for persistence. This high score indicates a high level of waterproof effect. Consequently, the biopolymer composition according to the present invention exhibits a strong waterproof effect, since it can withstand ten splashes of water after application to the skin.

[00161] In general, the above results demonstrate that the biopolymeric compositions according to the present invention exhibit important skin barrier effects. Example 6: Skin tolerance (patch test)

[00162] A test was performed by an independent laboratory to evaluate the local skin tolerance of a biopolymeric composition of Petition 870260082686, dated 08 / 14 / 2026, p. 71 / 182 67 / 72 in accordance with the present invention. The composition was of the same batch as the cellulose / chitosan / beeswax-based composition identified as Sample #8 in Example 3 (i.e., batch DEF23051502).

[00163] In summary, approximately 0.07 to 0.1 ml of the test product (i.e., biopolymeric composition) was applied to the intact skin of the back of 14 adult volunteers. The product was kept in contact with the skin for 48 hours under a semi-occlusive adhesive. Along with the test product, an empty negative control adhesive was also applied. The effects caused by the application of the test product were observed 15 minutes and 24 hours after removal of the adhesive to assess skin reactions, considering parameters such as erythema, edema, dryness / scaling, and vesicles, with severity scores ranging from 0 to 3.

[00164] The Average Irritation Index (MII) of the product under test was 0 in both periods (15 minutes and 24 hours after adhesive removal). Thus, the product under test was classified as non-irritating and dermatologically tested for skin compatibility according to the reference standard (data not shown). These results therefore confirm that the biopolymer compositions for skin care, according to the present invention, are dermatologically safe.

[00165] Similarly, Samples #10 and #11 (see Example 3 above) were submitted to an independent laboratory to assess local skin tolerance and both were found to be non-primary irritants after 48 hours in contact with the skin of volunteers. Example 7: Water retention effect - Composition based on cellulose / chitosan / beeswax

[00166] Transepidermal Water Loss (TEWL) is a very sensitive indicator of skin barrier integrity and can be measured Petition 870260082686, dated 08 / 14 / 2026, page 72 / 182 68 / 72 instrumentally by means of evaporimetry.

[00167] This study aimed to evaluate the effect of the cellulose / chitosan / beeswax-based composition identified as Sample # 8 in Example 3 (i.e., batch DEF23051502) on TEWL after 8 and 12 hours of application to the skin surface. The evaluation involved comparing the area analyzed at T8 hours ± 2 hours and T12 hours ± 2 hours with the TEWL measured at T0 (before product application). Measurements were performed using a Tewameter® TM300 (Courage-Khazaka GmbH, Germany).

[00168] The evaluation involved thirteen (13) volunteers. The product was applied epicutaneously in a non-occlusive manner, massaging until absorbed. The amount of product applied was approximately 2 mg / cm2, in an area of ​​35 cm2, according to the Colipa Guidelines. During the 12 hours of the study, the volunteers were instructed not to apply any other product and not to wash the examined area. Before each measurement with the Tewameter®, each volunteer was allowed to relax for approximately 15 minutes in an air-conditioned room to avoid abnormal collections due to excessive sweating or stress.

[00169] The product test lasted 12 hours for each of the 13 selected volunteers included in the study. Measurements with the Tewameter® were performed on the same treated area before product application (T0), 8 hours after product application (T8 hours ± 2 hours), and 12 hours after product application (T12 hours ± 2 hours). TEWL results are expressed as the mean percentage difference of the parameter measured at T8 hours ± 2 hours and T12 hours ± 2 hours compared to the parameter measured at T0. A statistical evaluation using Student's t-test was performed to assess the significance of the observed differences (p-values ​​< 0.05 were considered significant).

[00170] Under the experimental conditions adopted, the product in study Petition 870260082686, dated 08 / 14 / 2026, page 73 / 182 69 / 72 demonstrated a significant reduction in transepidermal water loss (TEWL) values ​​in the skin where the product was applied. As shown in Tables 22 and 23 below, the product showed a variation of -12.7% after T8 hours ± 2 hours and -19.0% after T12 hours ± 2 hours compared to T0. These variations were considered statistically significant (p-values ​​< 0.05) when comparing T8 hours ± 2 hours to T0 and when comparing T12 hours ± 2 hours to T0. Table 22: Average TEWL values ​​expressed in g / h / m2 TEMPO Batch DEF23051502 Control T0 8.78 8.75 T8 Hours ± 2 hours 7.66 8.7 T12 Hours ± 2 hours 7.10 8.73 Table 23: Percentage variation of average TEWL values Batch DEF23051502 Control T8 Hours ± 2 hours -12.7 -0.5 T12 Hours ± 2 hours -19 -0.2

[00171] Consequently, these results clearly demonstrate that the biopolymer compositions according to the present invention have positive effects on skin barrier integrity and are effective in reducing epidermal water loss and / or improving water retention by the skin. Example 7: Waterproof effect - Composition based on cellulose / chitosan / beeswax

[00172] The objective of this study was to demonstrate the waterproofing effect of a cellulose / chitosan / beeswax-based composition according to the invention. The product tested was the cellulose / chitosan / beeswax-based composition identified as Sample #8 in Example 3 (i.e., batch DEF23051502). The study involved Petition 870260082686, dated 08 / 14 / 2026, page 74 / 182 70 / 72 volunteers and the testing procedure was the same as Sample #9 in Example 5.

[00173] In this study, the two expert technicians assigned the tested product an average score of 9.7 out of 10 for product persistence after 10 splashes of water from the initial application. This high score indicates a high level of waterproof effect. Consequently, the biopolymer composition according to the present invention exhibits a strong waterproof effect, since it can withstand ten splashes of water after application to the skin. Example 8: Evaluating effectiveness on wrinkles and fine lines.

[00174] The cellulose / chitosan / beeswax-based composition identified as Sample #8 in Example 3 (i.e., batch DEF23051502) was tested in vivo to evaluate its effectiveness in removing wrinkles and fine lines. The study involved 13 volunteers and the testing procedure was the same as for Sample #9 in Example 4.

[00175] The study demonstrated that the tested product was highly effective in improving roughness and crow's feet wrinkles. In fact, the study observed a statistically significant reduction in wrinkles after application of the tested composition.

[00176] In particular, the average Rz parameter (Average Difference in Maximum Profile Height) showed a significant reduction with an average value of -5.9% after 15 minutes of product application (T15 Min). This value was considered statistically significant compared to T0 (p-value = 0.0027).

[00177] Consequently, these results clearly demonstrate that the biopolymer compositions according to the present invention are effective in reducing wrinkles and fine lines, and that this positive effect can be observed rapidly after application (e.g., within 15 minutes). Example 9: Comedogenicity assessment Petition 870260082686, dated 08 / 14 / 2026, page 75 / 182 71 / 72

[00178] The cellulose / chitosan-based composition identified as Sample #9 in Example 3 (i.e., batch DEF23041801) was tested in vivo to measure the likelihood of the product clogging skin pores.

[00179] The composition was evaluated using standard guidelines by an independent laboratory. The study results confirmed that the composition is non-comedogenic, meaning that the composition did not obstruct or block the skin pores of the volunteers. This is another major benefit of the biopolymer compositions of the present invention. Because they do not obstruct skin pores, the present compositions can help reduce the chances of acne and prevent the development of pimples. * * *

[00180] The headings are included herein for reference and to aid in locating certain sections. These headings are not intended to limit the scope of the concepts described herein, and these concepts may have applicability in other sections throughout the descriptive report. Thus, the present invention is not limited to the embodiments presented herein, but should have the broadest scope consistent with the principles and novel features described herein.

[00181] The singular forms a, an (a), an, an (an) and the, a include corresponding plural references, unless the context clearly indicates otherwise. Thus, for example, reference to a compound includes one or more such compounds and reference to the method includes reference to equivalent steps and methods known to those skilled in the art that could be modified or substituted for the methods described herein.

[00182] Unless otherwise indicated, all numbers expressing quantities of ingredients, reaction conditions, concentrations, properties, and so forth, used in the report Petition 870260082686, dated 08 / 14 / 2026, p. 76 / 182 72 / 72 descriptive and in the claims, should be understood as modified in all cases by the term approximately. At a minimum, each numerical parameter should be interpreted in light of the reported number of significant digits and by applying common rounding techniques. Consequently, unless otherwise indicated, the numerical parameters set forth in this descriptive report and the appended claims are approximations that may vary depending on the properties sought. Although the ranges and numerical parameters that define the broad scope of the embodiments are approximations, the numerical values ​​presented in the specific examples are reported to the greatest possible accuracy. Any numerical value, however, inherently contains certain errors resulting from variations in experiments, test measurements, statistical analyses, and others.

[00183] It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or alterations in light thereof will be suggested to persons skilled in the art and should be included in the present invention and within the scope of the appended claims. Petition 870260082686, dated 08 / 14 / 2026, p. 77 / 182

Claims

1 / 11 CLAIMS 1. Skin care composition, characterized in that it comprises: (i) biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension, wherein said stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent; and (ii) at least one of a humectant, an emollient, an occlusive, a ceramide, an emulsifier, a thickening agent, a preservative, an antioxidant, a fragrance, a functional ingredient, a UVB filter, a UVA filter and a broad-spectrum filter; and wherein said skin care composition is formulated for dermatologically safe application to human skin.

2. Skin care composition according to claim 1, characterized in that said composition provides at least one skin care benefit selected from the group consisting of: (i) smoothing the appearance and / or reducing the presence of fine lines and / or wrinkles; (ii) improving the appearance of under-eye bags and facial wrinkles; (iii) protecting the skin; (iv) lightening age spots; (v) mattifying the skin; (vi) regenerating and / or rejuvenating the skin; (vii) repairing and / or healing the skin; (viii) densifying the skin and / or improving one or more mechanical properties of the skin; (ix) improving skin elasticity; (x) creating a protective film on the skin with a minimum duration. Petition 870260082686, dated 08 / 14 / 2026, p.78 / 182 2 / 11 more than 24 hours; (xi) create a film on the skin that is almost dry to the touch; (xii) create a refreshing sensation on the skin; (xiii) hydrate and / or moisturize the skin; (xiv) prevent water evaporation from the skin; (xv) improve the integrity of the skin barrier; and (xvi) reduce epidermal water loss and / or improve water retention by the skin.

3. Skin care composition, characterized in that it comprises: (i) biopolymer molecules insoluble and / or semi-soluble in biopolymer that have been mechanically processed into a stable homogeneous aqueous suspension; and (ii) at least one of a humectant, an emollient, an occlusive, a ceramide, an emulsifier, a thickening agent, a preservative, an antioxidant, a fragrance, a functional ingredient, a UVB filter, a UVA filter and a broad-spectrum filter; wherein said skin care composition is formulated for dermatologically safe application to human skin; and wherein said skin care composition provides at least one skin care benefit selected from the group consisting of: (i) smoothing the appearance and / or reducing the presence of fine lines and / or wrinkles; (ii) improving the appearance of under-eye bags and facial wrinkles; (iii) protecting the skin; (iv) lightening age spots; (v) mattifying the skin;(vi) regenerate and / or rejuvenate the skin; Petition 870260082686, dated 08 / 14 / 2026, page 79 / 182 3 / 11 (vii) repair and / or heal the skin; (viii) densify the skin and / or improve one or more mechanical properties of the skin; (ix) improve skin elasticity; (x) create a protective film on the skin, lasting at least 24 hours; (xi) create a film on the skin that is almost dry to the touch; (xii) create a refreshing sensation on the skin; (xiii) hydrate and / or moisturize the skin; (xiv) prevent water evaporation from the skin; (xv) improve the integrity of the skin barrier; (xvi) reduce epidermal water loss and / or improve water retention by the skin; (xvii) non-comedogenic.

4. Skin care composition, according to any one of claims 1 to 3, characterized in that the biopolymer is selected from the group consisting of chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, fibroin, keratin, wool, alginic acid and mixtures thereof.

5. Skin care composition, according to any one of claims 1 to 3, characterized in that the biopolymer is selected from the group consisting of gelatin, pectin, starch, amylopectin, agarose, alginic acid, alginate, hyaluronic acid, RNA, DNA, xanthan gum, guar gum, latex, polymannans, suberin, cutin, cutana and mixtures thereof.

6. Skin care composition according to any one of claims 1 to 5, characterized in that the aqueous solvent is selected from the group consisting of: water, isopropanol, cetyl alcohol, ethanol, propanol, methanol, glycerol, acetic acid and mixtures thereof. Petition 870260082686, dated 14 / 08 / 2026, page 80 / 182 4 / 11 7. Skin care composition according to claim 6, characterized in that the humectant is selected from the group consisting of glycerin, urea, hyaluronic acid, salicylic acid, alpha-hydroxy acid, propylene glycol, honey, sorbitol, butylene glycol, sodium PCA, allantoin, sodium lactate, dicyanamide, tremella extract, seaweed, aloe vera and mixtures thereof.

8. Skin care composition according to claim 6 or 7, characterized in that the emollient is selected from the group consisting of petrolatum, lanolin, mineral oil, dimethicone and mixtures thereof.

9. Skin care composition according to any one of claims 6 to 8, characterized in that the occlusive is selected from the group consisting of mineral oil, petrolatum, lanolin, dimethicone, shea butter and mixtures thereof.

10. Skin care composition according to any one of claims 6 to 9, characterized in that the ceramide is selected from the group consisting of EOS ceramide, NS ceramide, NG ceramide, NP ceramide, AP ceramide, EOP ceramide, phytosphingosine, sphingosine and mixtures thereof.

11. Skin care composition, according to any one of claims 6 to 10, characterized in that the emulsifier is selected from the group consisting of glyceryl stearate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, tragacanthins, lecithin, mustard seed, soy lecithin, sodium phosphates, diacetyl tartaric acid ester of monoglyceride, sodium stearoyl lactylate and mixtures thereof.

12. Skin care composition, according to any one of claims 6 to 11, characterized in that the thickening agent is selected from the group consisting of aluminum and magnesium silicate, silica, bentonite, carbomer, cetyl palmitate, ammonium acryloyldimethyltaurate and mixtures thereof.

13. Skin care composition according to any one of claims 6 to 12, characterized in that the preservative is selected from the group consisting of phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, DMDM ​​hydantoin, quaternium-15, imidazolidinyl urea, diazolidinyl urea, polyoxymethylene urea, sodium hydroxymethylglycinate, 2-bromo-2-nitropropane-1,3-diol (bromopol), glyoxal, ethylparaben, butylparaben, methylparaben, propylparaben, isobutylparaben, isopropylparaben, benzylate, benzoic acid, benzyl ester, methylisothiazolinone (MIT; 2-methyl-4-isothiazolinone-3-one), methylchloroisothiazolinone (CMIT;5-chloro-2-methyl-4-isothiazolin-3-one), phenoxyethanol, 2-phenoxyethanol, Euxyl K™400 (mixture of phenoxyethanol and 1,2-dibromo-2,4-dicyanobutane), triclosan (TSC), triclocarban (TCC), benzyl alcohol, benzalkonium chloride, citric acid, dehydroacetic acid, essential oils, grapefruit seed extract, lactic acid, levulinic acid, potassium sorbate, sodium dehydroacetate, sodium metabisulfite, sodium salicylate, sorbic acid, vitamin E, zinc pyrithione and mixtures thereof.

14. Skin care composition, according to any one of claims 6 to 13, characterized in that the antioxidant is selected from the group consisting of vitamin A, vitamin C, vitamin E, vitamin B3, green tea extract, resveratrol, curcumin, lycopene, coenzyme Q10, polyphenols, C. asiatica extract, tamanu oil and mixtures thereof.

15. Skin care composition, according to any one of claims 6 to 14, characterized in that the fragrance is selected from the group consisting of linalool, citronellol, limonene, geraniol, eugenol, lavender oil (Lavandula angustifolia), rose flower extract (Rosa damascena), bergamot oil. Petition 870260082686, dated 08 / 14 / 2026, page 1. 82 / 182 6 / 11 (Citrus bergamia), ylang-ylang oil (Canaga odorata), lemon (Citrus limon), lime (Citrus aurantifolia or Citrus medica), orange (Citrus sinensis), tangerine (Citrus tangerine), peppermint (Mentha piperita), spearmint (Mentha spicata), eucalyptus, cinnamon (cinnamomum), cinnamaldehyde and mixtures thereof.

16. Skin care composition, according to any one of claims 6 to 15, characterized in that the functional ingredient is selected from the group consisting of biological extracts, vitamins, amino acids, peptides, proteins, mycosporines, Mycosporine-Like Amino Acids (MAAs), ultraviolet-absorbing imino compounds, essential fatty acids, hydroxy acids, beta-glucans and mixtures thereof.

17. Skin care composition according to any one of claims 1 to 16, characterized in that the stably dispersed biopolymer molecules have not been obtained by solubilization in an acidic solution or by solubilization in a basic solution.

18. Skin care composition according to any one of claims 1 to 3, characterized in that said skin care composition is selected from the following compositions: Table 1 - Composition A: FUNCTION(S) % (w / w) in the formulation biopolymer (dry weight) 0.3 - 10 Humectant 0 - 20 Emollient 1 - 15 Occlusive 0 - 10 Ceramide 0 - 5 Emulsifier 0.2 - 10 Thickening agent 0 - 5 Preservative 0.5 - 5 Petition 870260082686, dated 08 / 14 / 2026, page.Table 1 - Composition B: FUNCTION(S) % (w / w) in the formulation Antioxidant 0 - 0.5 Fragrance 0 - 0.2 Functional ingredient 0 - 10 UVB filter 0 - 20 UVA filter 0 - 20 Broad spectrum filter 0 - 20 Water (make up to 100%) 30-95 Table 2 - Composition B: FUNCTION(S) % (w / w) in the formulation Biopolymer (dry weight) 5 - 10 Preservative 0.5 - 5 Water to make 100% 85 - 94.5 Table 3 - Composition C: FUNCTION(S) % (w / w) in the formulation Biopolymer (dry weight) 5 - 10 Stabilizer 0.2 - 5 Water to make 100% 85-94.8 Table 4 - Composition D: FUNCTION(S) % (w / w) in the formulation Biopolymer (dry weight) 5 - 10 Emollient 0.2 - 1 Preservative 0.3 - 5 Stabilizer 0.2 - 1 Water to make 100% 83 - 94.3 Table 5 - Composition 3: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~7.2 Petition 870260082686, dated 08 / 14 / 2026, page.84 / 182 8 / 11 Ingredient % (w / w) in the formulation Water ~92.4 Sodium benzoate and potassium sorbate ~0.4 Table 6 - Composition 1: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~7.2 Water ~91.8 Glyceryl stearate ~0.3 Cetyl alcohol ~0.3 Sodium benzoate and potassium sorbate ~0.4 Table 7 - Composition 4: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~7.1 Water ~91.5 Glyceryl stearate ~0.3 Cetyl alcohol ~0.3 Beeswax ~0.3 Sodium benzoate and potassium sorbate ~0.4 Table 8 - Composition 2: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~7.1 Water ~91.8 Glyceryl stearate ~0.4 Cetyl alcohol ~0.3 Beeswax ~0.7 Sodium benzoate and potassium sorbate ~0.4 Table 9 - Composition 6: Ingredient % (w / w) in the formulation Petition 870260082686, dated 08 / 14 / 2026, page.Table 10 - Composition 5: Ingredient % (w / w) in the formulation Cellulose (dry weight) 5 Water 95 Table 11 - Composition 7: Ingredient % (w / w) in the formulation Cellulose (dry weight) 10 Water 90 Table 12 - Composition 8: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~5.7 alpha cellulose ~0.43 Preservative ~5.0 cetyl alcohol ~0.55 Beeswax ~0.93 Water to make 100% ~87.4 Table 13 - Composition 9: Ingredient % (w / w) in the formulation Chitosan (dry weight) ~5.0 alpha cellulose ~0.4 Preservative ~5.0 Water to make 100% ~89.

6.

19. Skin care composition, according to any one of claims 1 to 3, characterized in that said skin care composition consists of an instant skin softener. Petition 870260082686, dated 08 / 14 / 2026, page 86 / 182 10 / 11 20. Skin care composition according to claim 19, characterized in that the instant skin tensor further comprises one or more of the following: sodium silicate; polyol(s); clay(s); soft focus powder(s); polysaccharide(s); resin(s); natural-based polymer(s); synthetic polymer(s) and humectant(s).

21. Skin care composition, according to any one of claims 1 to 20, characterized in that it is for the skin of the hands, breasts, neck, arms, legs, eye contour, forehead, face and body.

22. Skin care composition according to any one of claims 1 to 21, characterized in that said skin care composition is formulated as a paste, an ointment, a cream, a lotion, a gel or a milk.

23. A method for protecting the skin, characterized in that it comprises applying a skin care composition to the skin, as defined in any one of claims 1 to 22.

24. A method for treating skin wrinkles, characterized in that it comprises applying a composition, as defined in any one of claims 1 to 21, to the skin surface of a human individual in need thereof.

25. Use of a composition, as defined in any one of claims 1 to 22, characterized in that it is for at least preventing the appearance of skin wrinkles and removing skin wrinkles.

26. Use of a composition, as defined in any of claims 1 to 22, characterized in that it is in Petition 870260082686, dated 08 / 14 / 2026, page 87 / 182 11 / 11 for the manufacture of a cosmetic for skin care.

27. Skin care kit, characterized in that it comprises two-part formulations that are activated when combined, wherein at least one of the two-part formulations comprises a skin care composition as defined in any one of claims 1 to 22. Petition 870260082686, dated 08 / 14 / 2026, pp. 88 / 182

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