COMPOSIÇÃO HERBICIDA E MÉTODO DE CONTROLE DE ERVAS DANINHAS

Abstract

The present invention provides a herbicide composition and a weed control method that have an excellent weed control effect. Provided is an herbicide composition comprising a 2,4-D-trolamine salt and at least one compound selected from compound group X. Compound group X consists of flumioxazin, trifludimoxazin, saflufenacil, tiafenacil, flufenoximacil, compounds represented by formula (I), and compounds represented by formula (II).

Description

1 / 76 HERBICIDE COMPOSITION AND WEED CONTROL METHOD [Technical Field]

[001] The present invention relates to a herbicidal composition and a method for controlling weeds. [Background of the Technique]

[002] Currently, many herbicides have been used for weed control and, for example, 2,4-D is known as an active ingredient for herbicides (see Non-Patent Document 1). Herbicidal compositions comprising a specific compound and 2,4-D or a salt thereof (a tri-isopropanolammium salt or a choline salt) are also known (see Patent Documents 1 to 3). [Prior Technique Document] [Patent Document]

[003] [Patent Document 1] JP 2-32004 A

[004] [Patent Document 2] WO 2015 / 094885

[005] [Patent Document 3] US 2016 / 0.058.000 A

[006] [Unpatented Document]

[007] [Unpatented Document 1] JA Turner, The Pesticide Manual, Nineteenth Edition, British Crop Production Council, 2021, pp. 304-310 [Description of the Invention] [Problems to be Solved by the Invention]

[008] One objective of the present invention is to provide a herbicidal composition with excellent weed control effect and a method for controlling them. [Means to Solve the Problems]

[009] It has been found that an excellent weed control effect is exerted by the use of a specific compound in Petition 870250084601, dated 09 / 19 / 2025, page 8 / 98 2 / 76 combination with a 2,4-D trolamine salt.

[0010] The present invention includes the following aspects.

[0011] 1. A herbicidal composition comprising at least one compound selected from the following group of compounds X and a salt of 2,4-D trolamine:

[0012] Group of compounds X: group consisting of flumioxazin, trifludimoxazin, saflufenacil, thiafenacil, flufenoximacyl, a compound represented by the following formula (I): and a compound represented by the following formula (II):

[0013] 2. The herbicidal composition according to 1, wherein the weight ratio of at least one compound selected from the group of compounds X to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:1 to 1:100.

[0014] 3. The herbicide composition according to 1, in which at least one compound selected from compound group X is flumioxazin.

[0015] 4. The herbicidal composition according to 3, wherein the weight ratio of flumioxazin to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:5 to 1:20.

[0016] 5. The herbicide composition according to 1, wherein Petition 870250084601, dated 09 / 19 / 2025, page 9 / 98 3 / 76 minus one compound selected from the group of compounds X is trifludimoxazine.

[0017] 6. The herbicidal composition according to 5, wherein the weight ratio of trifludimoxazine to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:100.

[0018] 7. The herbicide composition according to 1, in which at least one compound selected from group of compounds X is saflufenacil.

[0019] 8. The herbicidal composition according to 7, wherein the weight ratio of saflufenacil to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:50.

[0020] 9. The herbicide composition according to 1, in which at least one compound selected from the group of compounds X is thiafenacil.

[0021] 10. The herbicidal composition according to 9, wherein the weight ratio of thiafenacil to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:50.

[0022] 11. The herbicide composition according to 1, in which at least one compound selected from the group of compounds X is flufenoxymacyl.

[0023] 12. The herbicidal composition according to 11, wherein the weight ratio between flufenoxymacyl and an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:50.

[0024] 13. The herbicide composition according to 1, wherein at least one compound selected from the group of compounds X is a compound represented by formula (I).

[0025] 14. The herbicidal composition according to 13, wherein the weight ratio between the compound represented by formula (I) and an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:50. Petition 870250084601, dated 09 / 19 / 2025, page 10 / 98 4 / 76

[0026] 15. The herbicide composition according to 1, wherein at least one compound selected from the group of compounds X is a compound represented by formula (II).

[0027] 16. The herbicidal composition according to 15, wherein the weight ratio of the compound represented by formula (II) to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:100.

[0028] 17. A method for weed control, comprising a step of simultaneous or separate application, in any order, of at least one compound selected from the following group of compounds X and a salt of 2,4-D trolamine:

[0029] Group of compounds X: group consisting of flumioxazin, trifludimoxazin, saflufenacil, thiafenacil, flufenoximacyl, a compound represented by the following formula (I): and a compound represented by the following formula (II): FO CH, / = / NZFClΆ >7 \fe y—oo θ-\ ch30(II).

[0030] 18. The method according to 17, wherein the weight ratio of at least one compound selected from the group of compounds X to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:1 to 1:100.

[0031] 19. The method according to 17, in which at least one Petition 870250084601, dated 09 / 19 / 2025, page 11 / 98 Compound 5 / 76 selected from compound group X is flumioxazin.

[0032] 20. The method according to 19, wherein the weight ratio of flumioxazin to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:5 to 1:20.

[0033] 21. The method according to 17, wherein at least one compound selected from the group of compounds X is trifludimoxazine.

[0034] 22. The method according to 21, wherein the weight ratio of trifludimoxazine to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:100.

[0035] 23. The method according to 17, wherein at least one compound selected from the group of compounds X is saflufenacil.

[0036] 24. The method according to 23, wherein the weight ratio of saflufenacil to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:50.

[0037] 25. The method according to 17, wherein at least one compound selected from the group of compounds X is thiaphenacyl.

[0038] 26. The method according to 25, wherein the weight ratio of thiaphenacyl to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:50.

[0039] 27. The method according to 17, wherein at least one compound selected from the group of compounds X is flufenoxymacyl.

[0040] 28. The method according to 27, wherein the weight ratio of flufenoxymacyl to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:50.

[0041] 29. The method according to 17, wherein at least one compound selected from the group of compounds X is a compound represented by formula (I).

[0042] 30. The method according to 29, wherein the weight ratio of the compound represented by formula (I) to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to Petition 870250084601, dated 09 / 19 / 2025, page 12 / 98 6 / 76 1:50.

[0043] 31. The method according to 17, wherein at least one compound selected from the group of compounds X is a compound represented by formula (II).

[0044] 32. The method according to 31, wherein the weight ratio of the compound represented by formula (II) to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:100. [Effects of the Invention]

[0045] According to the present invention, it becomes possible to control weeds in a highly effective manner. [Method of Implementing the Invention]

[0046] The herbicidal composition of the present invention (hereinafter referred to as the present composition) comprises at least one compound selected from the group of compounds X (hereinafter referred to as compound X) and a salt of 2,4-D trolamine.

[0047] Flumioxazin is a compound mentioned on page 541 of the Pesticides Manual, Nineteenth Edition (hereinafter referred to as the Pesticides Manual), issued by the British Agricultural Production Council (BCPC). Trifludimoxazin is a compound mentioned on page 1204 of the Pesticides Manual. Saflufenacil is a compound mentioned on page 1065 of the Pesticides Manual. Thiapenacil is a compound mentioned on page 1164 of the Pesticides Manual. Flufenoxymacyl is a compound mentioned in document WO 2021 / 139482. The compound represented by formula (I) (hereinafter sometimes referred to as compound 1) is a compound mentioned in document WO 2016 / 095768. The compound represented by formula (II) (hereinafter sometimes referred to as compound 2) is a compound mentioned in document WO 2017 / 202768. 2,4-D trolamine salt is a 2,4-D salt mentioned on page 304 of the Pesticides Manual. Petition 870250084601, dated 09 / 19 / 2025, page 13 / 98 7 / 76 and is a compound mentioned on page 305 of the Pesticides Manual as 2,4-D trolamine. All of these compounds can be produced by a known method.

[0048] The present composition is generally a formulation prepared by mixing compound X and the 2,4-D trolamine salt with carriers, such as a solid carrier and a liquid carrier, and by adding formulation aids, such as a surfactant, as needed. The formulation type is preferably a suspension concentrate, an oil dispersion, a wettable powder, a water-dispersible granule, a granule, an oil-in-water emulsion, a water-in-oil emulsion, and an emulsifiable concentrate.

[0049] The total content of compound X and 2,4-D trolamine salt in the present composition is generally within a range of 0.01 to 99% by weight, and preferably 1 to 80% by weight. In the present descriptive report, the amount of 2,4-D trolamine salt is represented by an acid equivalent. In other words, in the present descriptive report, the amount of 2,4-D trolamine salt is the amount of 2,4-D (2,4-dichlorophenoxyacetic acid).

[0050] The weight ratio of compound X to the 2,4-D trolamine salt in the present composition is generally within a range of 1:1 to 1:100, and may, for example, be within a range of 1:1 to 1:50, 1:1 to 1:20, 1:5 to 1:100, 1:5 to 1:50, 1:5 to 1:20, 1:10 to 1:100, 1:10 to 1:50, 1:15 to 1:100, or 1:15 to 1:50.

[0051] Preferred examples of the weight ratio of compound X to the 2,4-D trolamine salt in the present composition include 1:1, 1:1.2, 1:1.5, 1:1.7, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:7, 1:10, 1:15, 1:20, 1:30, 1:40, 1:50, 1:75 and 1:100.

[0052] When compound X in the present composition is flumioxazin, the weight ratio of flumioxazin to the 2,4-D trolamine salt in the present composition is generally within a range Petition 870250084601, dated 09 / 19 / 2025, page 14 / 98 8 / 76 from 1:5 to 1:20.

[0053] Preferred examples of the weight ratio of flumioxazin to the 2,4-D trolamine salt in the present composition include 1:5, 1:7, 1:10, 1:15 and 1:20.

[0054] When compound X in the present composition is trifludimoxazine, the weight ratio of trifludimoxazine to the 2,4-D trolamine salt in the present composition is generally within a range of 1:15 to 1:100.

[0055] Preferred examples of the weight ratio of trifludimoxazine to the 2,4-D trolamine salt in the present composition include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75 and 1:100.

[0056] When compound X in the present composition is saflufenacil, the weight ratio of saflufenacil to the 2,4-D trolamine salt in the present composition is generally within a range of 1:15 to 1:50.

[0057] Preferred examples of the weight ratio of saflufenacil to the 2,4-D trolamine salt in the present composition include 1:15, 1:20, 1:30, 1:40 and 1:50.

[0058] When compound X in the present composition is thiaphenacil, the weight ratio of thiaphenacil to the 2,4-D trolamine salt in the present composition is generally within a range of 1:10 to 1:50.

[0059] Preferred examples of the weight ratio of thiaphenacyl to the 2,4-D trolamine salt in the present composition include 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50.

[0060] When compound X in the present composition is flufenoxymacyl, the weight ratio of flufenoxymacyl to the 2,4-D trolamine salt in the present composition is generally within a range of 1:10 to 1:50.

[0061] Preferred examples of the weight ratio of flufenoximacyl Petition 870250084601, dated 09 / 19 / 2025, page 15 / 98 9 / 76 for the 2,4-D trolamine salt in the present composition include 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50.

[0062] When compound X in the present composition is compound 1, the weight ratio of compound 1 to the 2,4-D trolamine salt in the present composition is generally within a range of 1:10 to 1:50.

[0063] Preferred examples of the weight ratio of compound 1 to the 2,4-D trolamine salt in the present composition include 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50.

[0064] When compound X in the present composition is compound 2, the weight ratio of compound 2 to the 2,4-D trolamine salt in the present composition is generally within a range of 1:15 to 1:100.

[0065] Preferred examples of the weight ratio of compound 2 to the 2,4-D trolamine salt in the present composition include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75 and 1:100.

[0066] The present composition is used to control weeds. The method of using the present composition is equivalent to the method mentioned below for controlling weeds in the present invention.

[0067] The present method can exert a synergistic herbicidal effect on a wide range of weeds, compared to the effect expected from the effects obtained when compound X and the 2,4-D trolamine salt are applied separately. Furthermore, the present composition can efficiently control a wide range of weeds in a cultivated field and in a vegetable garden, where normal cultivation is carried out with or without tillage, in an orchard and in an uncultivated area, without producing harmful effects that could be a problem for useful plants.

[0068] The present composition may include other active ingredients. Petition 870250084601, dated 09 / 19 / 2025, page 16 / 98 10 / 76 agrochemicals, for example, insecticides, nematicides and fungicides. Examples of these insecticides, nematicides and fungicides include neonicotinoid-based compounds, diamide-based compounds, carbamate-based compounds, organophosphate-based compounds, biological nematicides and other insecticides, as well as azole-based compounds, strobilurin-based compounds, metalaxyl-based compounds, SDHI compounds, other fungicides and plant growth regulators.

[0069] The weed control method of the present invention (hereinafter referred to as the present method) comprises a step of simultaneous or separate application, in any order, of a compound X and a 2,4-D trolamine salt (hereinafter referred to as the application step). The application step is carried out in a cultivated area, a vegetable garden, an orchard or an uncultivated area, and in the application step, compound X and the 2,4-D trolamine salt are applied to the location where the weeds are growing or will grow. When compound X and the 2,4-D trolamine salt are applied simultaneously, the present composition can be used, and a composition including compound X and a composition including the 2,4-D trolamine salt can be used in combination.When compound X and the 2,4-D trolamine salt are applied separately, the composition including compound X is applied first, followed by the composition including the 2,4-D trolamine salt, or these compositions are applied in reverse order.

[0070] The composition including compound X and the composition including the 2,4-D trolamine salt are generally formulations prepared by mixing compound X and the 2,4-D trolamine salt with carriers, such as a solid carrier and a liquid carrier, and by adding adjuvants to the formulation, such as a surfactant, as needed.

[0071] The method for applying compound X and the 2,4-D salt Petition 870250084601, dated 09 / 19 / 2025, page 17 / 98 11 / 76 trolamine includes, for example, a method for spraying compound X and the 2,4-D trolamine salt onto the soil (soil application) and a method for spraying them onto cultivated weeds (foliar application). Soil application and foliar application are generally carried out by spraying a spraying liquid obtained by mixing the present composition with water, using spraying equipment. The spray volume is generally in the range of 50 to 1,000 L / ha, but may, for example, be in the range of 100 to 500 L / ha or 140 to 300 L / ha. When compound X and the 2,4-D trolamine salt are sprayed separately, the composition including compound X and the composition including the 2,4-D trolamine salt are sprayed, respectively, according to the spraying method of the present composition.

[0072] The application rate of compound X and trolamine 2,4-D salt, as well as the total amount of compound X and trolamine 2,4-D salt, is generally within a range of 1 to 10,000 g / ha, but may, for example, be within a range of 2 to 5,000 g / ha or 5 to 2,000 g / ha.

[0073] The weight ratio of compound X to the 2,4-D trolamine salt in the present method is generally within a range of 1:1 to 1:100, and may, for example, be within a range of 1:1 to 1:50, 1:1 to 1:20, 1:5 to 1:100, 1:5 to 1:50, 1:5 to 1:20, 1:10 to 1:100, 1:10 to 1:50, 1:15 to 1:100 or 1:15 to 1:50.

[0074] Preferred examples of the weight ratio of compound X to the 2,4-D trolamine salt in the present method include 1:1, 1:1.2, 1:1.5, 1:1.7, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:7, 1:10, 1:15, 1:20, 1:30, 1:40, 1:50, 1:75 and 1:100.

[0075] When compound X in the present method is flumioxazin, the weight ratio of flumioxazin to the 2,4-D trolamine salt in the present method is generally within a range of 1:5 to 1:20. Petition 870250084601, dated 09 / 19 / 2025, page 18 / 98 12 / 76

[0076] Preferred examples of the weight ratio of flumioxazin to the 2,4-D trolamine salt in the present method include 1:5, 1:7, 1:10, 1:15 and 1:20.

[0077] When compound X in the present method is trifludimoxazine, the weight ratio of trifludimoxazine to the 2,4-D trolamine salt in the present method is generally within a range of 1:15 to 1:100.

[0078] Preferred examples of the weight ratio of trifludimoxazine to the 2,4-D trolamine salt in the present method include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75 and 1:100.

[0079] When compound X in the present method is saflufenacil, the weight ratio of saflufenacil to the 2,4-D trolamine salt in the present method is generally within a range of 1:15 to 1:50.

[0080] Preferred examples of the weight ratio of saflufenacil to the 2,4-D trolamine salt in the present method include 1:15, 1:20, 1:30, 1:40 and 1:50.

[0081] When compound X in the present method is thiaphenacil, the weight ratio of thiaphenacil to the 2,4-D trolamine salt in the present method is generally within a range of 1:10 to 1:50.

[0082] Preferred examples of the weight ratio of thiaphenacyl to the 2,4-D trolamine salt in the present method include 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50.

[0083] When compound X in the present method is flufenoxymacyl, the weight ratio of flufenoxymacyl to the 2,4-D trolamine salt in the present method is generally within a range of 1:10 to 1:50.

[0084] Preferred examples of the weight ratio of flufenoximacyl to the 2,4-D trolamine salt in the present method include 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50.

[0085] When compound X in the present method is compound Petition 870250084601, dated 09 / 19 / 2025, page 19 / 98 13 / 76 1. The weight ratio of compound 1 to the 2,4-D trolamine salt in the present method is generally within a range of 1:10 to 1:50.

[0086] Preferred examples of the weight ratio of compound 1 to the 2,4-D trolamine salt in the present method include 1:10, 1:15, 1:20, 1:30, 1:40 and 1:50.

[0087] When compound X in the present method is compound 2, the weight ratio of compound 2 to the 2,4-D trolamine salt in the present method is generally within a range of 1:15 to 1:100.

[0088] Preferred examples of the weight ratio of compound 2 to the 2,4-D trolamine salt in the present method include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75 and 1:100.

[0089] The spray liquid to be sprayed in the present method may include an adjuvant. The type of adjuvant is not particularly limited, and when oil-based adjuvants, such as Agri-Dex and MSO (mineral oils, such as paraffin-based hydrocarbons, naphthene-based hydrocarbons and aromatic hydrocarbons, or methylated seed oils obtained by esterification of vegetable oils (soybean oil and rapeseed oil)) are included in the spray liquid, the concentration of the adjuvant in the spray liquid is 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5% or 6% (volume / volume). When non-ionic adjuvants, such as Induce (polyoxyalkylene alkyl ether, polyoxyalkylene fatty acid ester, alkyl aryl alkoxylate or polyoxyalkylene glycol) are included in the spray liquid, the concentration of the adjuvant in the liquid is The spraying concentration is 0.05%, 0.1%, 0.25%, or 0.5%.Examples of other adjuvants include anionic adjuvants, such as Gramin S (substituted sulfonate), cationic adjuvants, such as Genamin T 200BM (polyoxyethyleneamine), and organosilicon-based adjuvants, such as Silwett L77. The spray liquid to be sprayed in the present method may also include a drift-reducing agent, such as... Petition 870250084601, dated 09 / 19 / 2025, page 20 / 98 14 / 76 Intact (polyethylene glycol).

[0090] The pH and hardness of the spray liquid prepared in the present method are not particularly limited, and the pH is generally in the range of 5 to 9 and the hardness is generally in the range of 0 to 500 ppm, like the American hardness.

[0091] The application period of compound X and 2,4-D trolamine salt is not particularly limited, and the period is generally from 5 am to 9 pm, with photon flux density generally between 10 and 2,500 μmol / m2 / second, the temperature is generally from 0 to 35 degrees Celsius and the wind speed is generally 3 MPH or less.

[0092] The spray pressure to be used for the application of compound X and 2,4-D trolamine salt is not particularly limited, generally being from 30 (2.10 kg / cm2) to 120 PSI (8.43 kg / cm2), and preferably within a range of 40 (2.81 kg / cm2) to 80 PSI (5.62 kg / cm2).

[0093] The cultivation field where the application step is carried out may include a food crop field, such as a peanut field, a soybean field (indeterminate growth habit, determinate growth habit, semi-determinate growth habit), a maize field (dent maize, flint maize, flour maize, popcorn, waxy maize, sweet maize, field maize) and a wheat field (bread wheat (soft wheat, durum wheat, medium wheat, red wheat, white wheat), durum wheat, spelt wheat, club wheat and, winter habit and spring habit thereof), a barley field (two-row barley, six-row barley, hulled barley field, waxy barley and, winter habit and spring habit thereof), a forage crop field, such as a sorghum field and an oat field, an industrial crop field, such as a cotton field (uproot cotton, pima cotton) and a rapeseed field, a canola field (winter habit, indeterminate growth habit) (spring) and Petition 870250084601, dated 09 / 19 / 2025, page 21 / 98 15 / 76 sugar crops, such as a sugarcane field and a beet field.

[0094] The vegetable field where the application step is carried out may include a field for the cultivation of solanaceous vegetables (eggplant, tomato, green pepper, chili pepper, potato, etc.), a field for the cultivation of cucurbitaceous vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.), a field for the cultivation of cruciferous vegetables (radish, turnip, horseradish, kohlrabi, Chinese cabbage, cabbage, mustard, broccoli, cauliflower, etc.), a field for the cultivation of asteraceous vegetables (burdock, daisy, artichoke, lettuce, etc.), a field for the cultivation of liliaceae vegetables (chives, onion, garlic, asparagus, etc.), a field for the cultivation of umbelliferous vegetables (carrot, parsley, celery, etc.) A field for cultivating chenopodiaceae vegetables (spinach, chard, etc.), a field for cultivating lamiaceae vegetables (perilla, mint, basil, lavender, etc.), a strawberry field, a sweet potato field, a yam field, a taro field, etc.

[0095] The orchard area where the application step is carried out may include an orchard, a tea field, a mulberry field, a coffee field, a banana field, a palm field, a flowering tree farm, a flowering tree field, a seedling field, a nursery field, a wooded area or a garden. The orchard tree may include pome fruits (apple, pear, Japanese pear, Chinese quince, quince, etc.), stone fruits (peach, plum, nectarine, Japanese apricot, yellow peach, apricot, prune, etc.), citrus fruits (citrus unshiu, orange, lemon, lime, grapefruit, etc.), nuts (chestnut, walnut, hazelnut, almond, pistachio, cashew nut, macadamia nut, etc.), berries (grape, blueberry, cranberry, blackberry, raspberry, etc.), persimmon, olive, loquat, etc.

[0096] The uncultivated area where the application step is carried out Petition 870250084601, dated 09 / 19 / 2025, page 22 / 98 16 / 76 may include an athletics field, a vacant lot, the edge of a railway line, a park, a parking area, the edge of a road, a dry riverbed, under power lines, a building site, a factory, etc.

[0097] The crops grown in a field where the application step is carried out are not limited, provided that their varieties are varieties generally grown as crops.

[0098] The plant of the variety mentioned above may be a plant that can be produced by natural hybridization, a plant that may occur as a result of a mutation, an F1 hybrid plant, or a transgenic plant (also called a genetically modified plant). These plants generally exhibit properties such as conferring tolerance to a herbicide, accumulating a toxic substance against pests, suppressing susceptibility to a plant disease, increasing yield potential, increasing resistance to a biological or non-biological stress factor, accumulating a substance, and improving storage or processability.

[0099] The term F1 hybrid plant refers to a first-generation filial plant produced by hybridizing two different varieties, generally exhibiting a superior characteristic to either of its progenitors, i.e., possessing hybrid vigor. The term transgenic plant refers to a plant produced by introducing a foreign gene from another organism, such as a microorganism, into a plant, possessing a property that cannot be easily acquired through hybridization, mutation induction, or natural recombination in a natural environment.

[00100] Examples of techniques for producing the plants mentioned above include a conventional breeding technique, a technique Petition 870250084601, dated 09 / 19 / 2025, page 23 / 98 17 / 76 transgenic technique, a genome-based breeding technique, a novel breeding technique, and a genome editing technique. Conventional breeding is a technique for producing a plant with a desirable property by mutation or hybridization. Transgenic technique is a technique for conferring a new property to a specific organism (e.g., a microorganism) by isolating a gene (DNA) of interest from the organism and then introducing the gene (DNA) into the genome of another target organism, and an antisense technique or RNA interference technique, which is a technique for conferring a new or improved property to a plant by silencing another gene that occurs in the plant. Genome-based breeding is a technique for increasing breeding efficiency using genomic information and includes DNA marker breeding (also called genomic marker or genetic marker) and genomic selection.For example, genetic improvement with DNA markers is a method in which offspring with a desired useful trait gene are selected from among many hybrid offspring using a DNA marker, which is a DNA sequence capable of serving as an indicator of the position of a specific useful trait gene in a genome. Analyzing a hybrid offspring of a plant at the seedling stage using a DNA marker has such a characteristic that it makes it possible to shorten the time required for effective genetic improvement.

[00101] Genomic selection is a technique that produces a prediction equation from a phenotype and previously obtained genomic information, and then a property is predicted from the prediction equation and the genomic information, without performing phenotypic evaluation. Genomic selection can contribute to increasing the efficiency of genetic improvement. A new technique of Petition 870250084601, dated 09 / 19 / 2025, p. 24 / 98 18 / 76 Genetic improvement is a collective term for combinations of genetic improvement techniques, including molecular biology techniques. Examples of new genetic improvement techniques include techniques such as cisgenesis / intragenesis, oligonucleotide-directed mutagenesis, RNA-dependent DNA methylation, genome editing, grafting onto rootstock or genetically modified grafts, reverse genetic improvement, agroinfiltration, and seed production technology (SPT). Genome editing is a technique that converts genetic information in a sequence-specific manner and allows for the deletion of a base sequence, the substitution of an amino acid sequence, the introduction of a foreign gene, and similar changes.Examples of tools for the technique include zinc finger nuclease (ZFN), TALEN, CRISPR / Cas9, CRISPRZCpfl, and meganuclease, which can cleave DNA in a sequence-specific manner, and a sequence-specific genomic modification technique using CAS9 nickase, Target-AID, and similar tools, which is produced by any of the modifications of the tools mentioned above.

[00102] Examples of the plants mentioned above include plants listed in the Genetically Modified Crops Registration Database (GM APPROVAL DATABASE) on an electronic information website at the INTERNATIONAL SERVICE FOR THE ACQUISITION OF AGRI-BIOTECHNOLOGY APPLICATIONS, ISAAA (http: / / www.isaaa.org / ). More specific examples of plants include a herbicide-tolerant plant, a pest-resistant plant, a disease-resistant plant, a plant whose quality (e.g., increased or decreased content or altered composition) of a product (e.g., starch, amino acid, fatty acid, etc.) is modified, a plant with a modified fertility characteristic, a plant intolerant to biological stresses, or a plant whose Petition 870250084601, dated 09 / 19 / 2025, page 25 / 98 19 / 76 characteristic associated with growth or productivity is modified.

[00103] The plant to which herbicide tolerance has been conferred by a transgenic technique also includes plants, each of which, by a transgenic technique, are tolerant to: a 4-hydroxyphenylpyruvate dioxygenase inhibitor (hereinafter abbreviated as HPPD), such as isoxaflutol and mesotrione; an acetolactate synthase inhibitor (hereinafter abbreviated as ALS), such as an imidazolinone-type herbicide, including imazethapyr, and a sulfonylurea-type herbicide, including thifoliussulfuron-methyl; a 5-enolpyruvylshikimate-3-phosphate synthase inhibitor (hereinafter abbreviated as EPSPS), such as glyphosate; a glutamine synthase inhibitor, such as glufosinate; an auxin-type herbicide, such as 2,4-D and dicamba; an oxinyl-type herbicide, including bromoxynil; and a protoporphyrinogen oxidase inhibitor (hereinafter abbreviated as PPO), such as flumioxazin.Preferred examples of herbicide-tolerant transgenic plants include: a cereal such as wheat, barley, rye and oats; canola, sorghum, soybeans, rice, rapeseed, beet, sugarcane, grapes, lentils, sunflowers, alfalfa, pome fruits, stone fruits, coffee, tea, strawberries, wheatgrass and a vegetable such as tomato, potato, cucumber and lettuce; more preferably, a cereal such as wheat, barley, rye and oats, soybeans, rice, grapevines, tomatoes, potatoes and a pome fruit.

[00104] Specific examples of herbicide-tolerant plants will be mentioned below.

[00105] Plants tolerant to glyphosate-based herbicides; produced by introducing at least one glyphosate-tolerant EPSPS gene originating from the CP4 strain of Agrobacterium tumefaciens (CP4 epsps), a glyphosate-metabolizing enzyme gene in which metabolic activity is increased by a shuffling technique for a glyphosate-metabolizing enzyme gene (glyphosate N Petition 870250084601, dated 09 / 19 / 2025, p. 26 / 98 20 / 76 acetyltransferase) originating from Bacillus licheniformis (gat4601, gat4621), a glyphosate-metabolizing enzyme originating from the LBAA strain of Ochrobacterum anthropi (glyphosate oxidase gene, goxv247), or an EPSPS gene originating from maize and having a glyphosate tolerance mutation (mepsps, 2mepsps). Examples of major plants include alfalfa (Medicago sativa), Argentine canola (Brassica napus), cotton (Gossypium hirsutum L.), creeping grass (Agrostis stolonifera), maize (Zea mays L.), Polish canola (Brassica rapa), potato (Solanum tuberosum L.), soybean (Glycine max L.), sugar beet (Beta vulgaris), and wheat (Triticum aestivum). Some glyphosate-tolerant transgenic plants are commercially available.For example, a genetically modified plant expressing glyphosate-tolerant EPSPS originating from Agrobacterium is commercially available under trade names including the trademark 'Roundup Ready'; a genetically modified plant expressing a glyphosate-metabolizing enzyme originating from Bacillus in which metabolic activity is increased by a shuffling technique is commercially available under the trade names Optimum (trademark) GAT (trademark), Optimum (trademark) Gly canola and similar; and a genetically modified plant expressing EPSPS having a glyphosate tolerance mutation originating from maize is commercially available under the trademark GlyTol (trademark).

[00106] Plants tolerant to glufosinate herbicides; produced by introducing at least one gene (bar) for phosphinothricin N-acetyltransferase (PAT), which is a glufosinate-metabolizing enzyme originating from Streptomyces hygroscopicus, one gene (pat) for a phosphinothricin N-acetyltransferase (PAT) enzyme, which is a glufosinate-metabolizing enzyme originating from Streptomyces viridochromogenes, or a synthetic pat gene (pat syn) originating from the strain Petition 870250084601, dated 09 / 19 / 2025, page 27 / 98 21 / 76 Tu494 from Streptomyces viridochromogenes. Examples of the parent plant include Argentine canola (Brassica napus), chicory (Cichorium intybus), cotton (Gossypium hirsutum L.), maize (Zea mays L.), Polish canola (Brassica rapa), rice (Oryza sativa L.), soybean (Glycine max L.), and sugar beet (Beta vulgaris). Some genetically modified glufosinate-tolerant plants are commercially available. The glufosinate-metabolizing enzyme (bar) originating from Streptomyces hygroscopicus and a genetically modified plant originating from Streptomyces viridochromogenes are commercially available under the trade names LibertyLink (registered trademark), InVigor (registered trademark), and WideStrike (registered trademark).

[00107] Plants tolerant to oxynil-type herbicides (e.g., bromoxynil); include transgenic plants tolerant to oxynil-type herbicides, e.g., bromoxynil, in which a nitrilase gene (bxn), which is an enzyme metabolizing oxynil-type herbicides (e.g., bromoxynil), originated from Klebsiella pneumoniae subsp. Examples of the parent plant include Argentine canola (Brassica napus), cotton (Gossypium hirsutum L.) and tobacco (Nicotiana tabacum L.), which are commercially available under the trade names Navigator (registered trademark) canola or BXN (registered trademark).

[00108] Plants tolerant to ALS inhibitors; carnation (Dianthus caryophyllus) having introduced into it an ALS inhibitor-tolerant gene (surB) originating from tobacco (Nicotiana tabacum) as a selection marker is commercially available under the trade names Moondust (registered trademark), Moonshadow (registered trademark), Moonshade (registered trademark), Moonlite (registered trademark), Moonaqua (registered trademark), Moonvista (registered trademark), Moonique (registered trademark), Moonpearl (registered trademark), Moonberry (registered trademark) and Moonvelvet (registered trademark); flax (Linum usitatissumum L.) having introduced into it an ALS-tolerant gene. Petition 870250084601, dated 09 / 19 / 2025, page 28 / 98 22 / 76 ALS inhibitor (als) derived from watercress (Arabidopsis thaliana) is commercially available under the CDC trademark Triffid Flax; corn (Zea mays L.Soybeans, having tolerance to a sulfonylurea herbicide and an imidazolinone herbicide, having introduced into them an ALS inhibitor-tolerant gene (zm-hra) originating from corn, are commercially available under the trademarks Optimum (trademark) GAT (trademark); Soybeans, having tolerance to an imidazolinone herbicide, having introduced into them an ALS inhibitor-tolerant gene (csr1-2) originating from creeping cress, are commercially available under the trademark Cultivance; And soybeans, having tolerance to a sulfonylurea herbicide, having introduced into them an ALS inhibitor-tolerant gene (gm-hra) originating from soybeans (Glycine max), are commercially available under the trademarks Treus (trademark), Plenish (trademark) and Optimum GAT (trademark). Cotton, having introduced into it an ALS inhibitor-tolerant gene. (S4-HrA) originating from tobacco (Nicotiana tabacum cv.Xanthi) can also be mentioned.

[00109] HPPD inhibitor-tolerant plants; soybean, in which both a mesotrione-tolerant HPPD gene (avhppd03) originating from oats (Avena sativa) and a gene (pat) for a phosphinothricin N-acetyltransferase (PAT) enzyme, which is a glufosinate-metabolizing enzyme originating from Streptomyces viridochromogenes, are introduced, is commercially available under the registered trademark Herbicide-tolerant soybean line.

[00110] 2,4-D tolerant plants; maize containing, introduced into it, an aryloxyalkanoate dioxygenase (aad-1) gene, which is a 2,4-D metabolizing enzyme originating from Sphingobium herbicidavorans, is commercially available under the trademark Enlist (trademark) Maize. Soybeans and cotton containing, introduced into them, Petition 870250084601, dated 09 / 19 / 2025, p. 29 / 98 23 / 76, an aryloxyalkanoate dioxygenase (aad-12) gene, which is a 2,4-D metabolizing enzyme originating from Delftia acidovorans, is commercially available under the trademark Enlist (trademark) Soybean.

[00111] Dicamba-tolerant plants; includes soybean and cotton containing, introduced into them, a dicamba monooxygenase (dmo) gene, which is a dicamba-metabolizing enzyme originating from the DI-6 strain of Stenotrophomonas maltophilia. Soybean (Glycine max L.), in which a glyphosate-tolerant EPSPS gene (CP4 epsps) originating from the CP4 strain of Agrobacterium tumefaciens is also introduced simultaneously with the aforementioned gene, is commercially available as Genuity (registered trademark), Roundup Ready (registered trademark) 2 Xtend (registered trademark).

[00112] The PPO inhibitor-tolerant culture in the present invention includes a culture conferred with the ability to produce PPO with reduced affinity to the PPO inhibitor by means of a transgenic technique and a culture conferred with the ability to detoxify / decompose the PPO inhibitor by cytochrome P450 monooxygenase by means of a transgenic technique. The PPO inhibitor-tolerant culture may be a culture that is endowed with the ability to produce PPO with reduced affinity to the PPO inhibitor and the ability to detoxify / decompose the PPO inhibitor by cytochrome P450 monooxygenase by means of a transgenic technique. These tolerant crops are mentioned, for example, in Patent Documents such as WO 2011085221, WO 2012080975, WO 2014030090, WO 2015022640, WO 2015022636, WO 2015022639, WO 2015092706, WO 2016203377, WO 2017198859, WO 2018019860, WO 2018022777, WO 2017112589, WO 2017087672, WO 2017039969, WO 2017023778, WO 2018022777, WO 2019118726 and Non-Patent Document (Science of handling of Petition 870250084601, dated 09 / 19 / 2025, page 30 / 98 24 / 76 pests, 61, 2005, 277-285).

[00113] Examples of commercially available transgenic plants with herbicide tolerance include Roundup Ready Corn, Roundup Ready 2, Agrisure GT™, Agrisure GT / CB / LL™, Agrisure GT / RW™, Agrisure 3000GT™, YieldGard VT Rootworm / RR2™, and YieldGard VT Triple™, each glyphosate-tolerant; Roundup Ready Soybean and Optimum GAT™, each glyphosate-tolerant; Roundup Ready Cotton and Roundup Ready Flex™, each glyphosate-tolerant; Roundup Ready Canola™, glyphosate-tolerant; Roundup Ready Alfalfa™, glyphosate-tolerant; and Roundup Ready Rice™, glyphosate-tolerant; Roundup Ready 2, Liberty Link, Herculex 1, Herculex RW™, Herculex Xtra™, Agrisure GT / CB / LL™, and Agrisure CB / LL / RW and Bt10, each with glufosinate tolerance; FiberMax Liberty Link cotton with glufosinate tolerance; Liberty Link Rice with glufosinate tolerance; in Vigor canola with glufosinate tolerance;BXN cotton with bromoxynil tolerance; and Navigator® and Compass canola with bromoxynil tolerance. Other plants that are herbicide-modified are also widely known, such as: alfalfa, apple, barley, eucalyptus, flax, grape, lentil, rapeseed, pea, potato, rice, sugar beet, sunflower, tobacco, tomato, grass and wheat, each having glyphosate tolerance (see, for example, US 5,188,642, US 4,940,835, US 5,633,435, US 5,804,425 and US 5,627,061); Beans, cotton, soybeans, peas, potatoes, sunflowers, tomatoes, tobacco, corn, sorghum, and sugarcane, each tolerant to dicamba (see, for example, WO 2008051633, US 7,105,724, and US 5,670,454); soybeans, sugar beets, potatoes, tomatoes, and tobacco, each tolerant to glufosinate (see, for example, US 6,376,754, US 5,646,024, and US 5,561,236); cotton, peppers, apples, tomatoes, sunflowers, tobacco, potatoes, corn, cucumbers, wheat; Petition 870250084601, dated 09 / 19 / 2025, page 31 / 98 25 / 76 soybeans, sorghum and millet, each having tolerance to 2,4-D (see, for example, US 6,153,401, US 6,100,446, WO 2005107437, US 5,608,147 and US 5,670,454); canola, corn, millet, barley, cotton, brown mustard, lettuce, lentils, cantaloupe, foxtail millet, oats, rapeseed, potatoes, rice, rye, sorghum, soybeans, sugar beets, sunflowers, tobacco, tomatoes, and wheat, each tolerant to an ALS inhibitor (e.g., a sulfonylurea-type herbicide and an imidazolinone-type herbicide) (see, for example, US 5013659, WO 2006060634, US 4,761,373, US 5,304,732, US 6,211,438, US 6,211,439, and US 6,222).100) (particularly, rice tolerant to an imidazolinone-type herbicide; rice with a specific mutation (e.g., S653N, S654K, A122T, S653(At)N, S654(At)K, A122(At)T) in an acetolactate synthase gene (acetohydroxyacid synthase gene) and similar (see, for example, US 2003 / 0217381, WO 2005 / 20673)); Barley, sugarcane, rice, corn, tobacco, soybean, cotton, rapeseed, sugar beet, wheat, and potato, each tolerant to an HPPD inhibitor (e.g., an isoxazole-type herbicide such as isoxaflutol; a triketone-type herbicide such as sulcotrione and mesotrione; a pyrazole-type herbicide such as pyrazolinate and diketonitrile, which is a decomposition product of isoxaflutol) (see, for example, WO 2004 / 055191, WO 199638567, WO 1997049816, and US 6,791,014).

[00114] Examples of a plant that has been endowed with tolerance to a herbicide by a classical breeding technique or a genome-based breeding technique include: Clearfield Rice rice, Clearfield Wheat, Clearfield Sunflower, Clearfield Lentils and Clearfield Canola (a product manufactured by BASF), each having tolerance to an imidazolinone-type ALS inhibitor such as imazethapyr and imazamox; STS Soybean having tolerance to a sulfonylurea-type ALS inhibitor such as thifoliussulfuron-methyl; Petition 870250084601, dated 09 / 19 / 2025, page 32 / 98 26 / 76 Sethoxydim-tolerant corn SR corn and Poast Protected (trademark) corn having tolerance to an acetyl CoA carboxylase inhibitor, such as a trione oxime type herbicide and an aryloxyphenoxypropionate type herbicide; for example, Sunflower ExpressSun (trademark) having tolerance to a sulfonylurea type herbicide, such as tribenurone; Rice Rrovisia (trademark) Rice with tolerance to an acetyl CoA carboxylase inhibitor, such as quizalofop; Canola Triazine Tolerant Canola with tolerance to a PSII inhibitor.

[00115] An example of a plant that is endowed with herbicide tolerance by a genome editing technique is canola SU Canola (registered trademark) with tolerance to a sulfonylurea-type herbicide and produced by the Rapid Trait Development System (RTDS (registered trademark)). RTDS (registered trademark) corresponds to an oligonucleotide-directed mutagenesis employed in a genome editing technique and is a technique by which it becomes possible to introduce a mutation into a plant by means of a Genetic Repair Oligonucleotide (GRON), i.e., a chimeric DNA-RNA oligonucleotide, without the need to cleave the DNA in the plant. Other examples of the plant also include: maize, whose herbicide tolerance and phytic acid content are reduced as a result of deletion of the endogenous IPK1 gene using a zinc finger nuclease (see, for example, Nature 459, 437-441 2009); and rice, which is given herbicide tolerance using CRISPR / Cas9 (see, for example, Rice, 7, 5 2014).

[00116] The technique that confers herbicide tolerance through a new breeding technique includes a breeding technique that employs grafting and, as an example of the transmission of a characteristic from a genetically modified rootstock to a scion, one can mention soybeans, in which glyphosate tolerance is conferred to a non-transgenic soybean scion using Roundup Ready (registered trademark) glyphosate-tolerant soybeans as rootstock. Petition 870250084601, dated 09 / 19 / 2025, page 33 / 98 27 / 76 graft (see Weed Technology 27:412-416 2013).

[00117] The plants mentioned above also include a lineage to which two or more properties of a parental lineage are conferred, such as tolerance to non-biological stress, resistance to plant diseases, tolerance to herbicides, resistance to pests, growth or yield characteristics, nutrient uptake, product quality, fertility characteristics and the like, as mentioned above, using a transgenic technique, a classical breeding technique, a genome-based breeding technique, a novel breeding technique or genome editing technique or the like, and a lineage to which two or more properties of a parental lineage are conferred by crossing plants of the same species or with different properties.

[00118] Examples of a commercially available plant with tolerance to two or more herbicides include: GlyTol (trademark) LibertyLink (trademark) cotton and GlyTol (trademark) LibertyLink (trademark), each tolerant to glyphosate and glufosinate; Roundup Ready (trademark) LibertyLink (trademark) Maize corn, tolerant to glyphosate and glufosinate; Enlist (trademark) Soybean, tolerant to glufosinate and 2,4-D; Genuity (trademark) Roundup Ready (trademark) 2 Xtend (trademark) soybean, tolerant to glyphosate and dicamba; OptimumGAT (trademark) corn and soybean, tolerant to glyphosate and an ALS inhibitor; Enlist E3 (trademark) and Enlist (trademark) Roundup Ready 2 Yield (trademark) genetically modified soybeans, each tolerant to three herbicides: glyphosate, glufosinate, and 2,4-D;Genetically modified corn Enlist (registered trademark) Roundup Ready (registered trademark) Corn 2; Petition 870250084601, dated 09 / 19 / 2025, page 34 / 98 28 / 76 with tolerance to glyphosate, 2,4-D and aryloxyphenoxypropionate (FOP) herbicides; genetically modified corn Enlist (registered trademark) Roundup Ready (registered trademark) Corn 2, with tolerance to glyphosate, 2,4-D and aryloxyphenoxypropionate (FOP) herbicides; genetically modified cotton Bollgard II (registered trademark) XtendFlex (registered trademark) Cotton, with tolerance to dicamba, glyphosate and glufosinate; and genetically modified cotton Enlist (registered trademark) Cotton with tolerance to three herbicides: glyphosate, glufosinate and 2,4-D.In addition, the following plants are also being developed: cotton tolerant to glufosinate and 2,4-D; cotton tolerant to both glufosinate and dicamba; corn tolerant to both glyphosate and 2,4-D; soybeans tolerant to both glyphosate and an HPPD herbicide; and genetically modified corn tolerant to glyphosate, glufosinate, 2,4-D, aryloxyphenoxypropionate (FOP) type herbicides, and cyclohexadione (DIM) type herbicides.

[00119] Examples of commercially available plants with herbicide tolerance and pest resistance include: YieldGard Roundup Ready and YieldGard Roundup Ready 2 corn, each with glyphosate tolerance and corn borer resistance; Agrisure CB / LL corn with glufosinate tolerance and corn borer resistance; YieldGard VT Root worm / RR2 corn with glyphosate tolerance and corn rootworm resistance; YieldGard VT Triple corn with glyphosate tolerance and resistance to corn rootworm and corn borer; Herculex I corn with glufosinate tolerance and resistance to a lepidopteran pest (CrylF) (e.g., resistance to western bean leafcutter, corn borer, black leafworm, and fall armyworm); YieldGard Corn Rootworm / Roundup Ready 2 corn with glyphosate tolerance and resistance to corn rootworm; Agrisure GT / RW corn with tolerance to Petition 870250084601, dated 09 / 19 / 2025, page 35 / 98 29 / 76 glufosinate and resistance to a coleopteran pest for maize (Cry3A) (e.g., resistance to western maize rootworm, northern maize rootworm, and Mexican maize rootworm); Herculex RW maize with glufosinate tolerance and resistance to a coleopteran pest (Cry34 / 35Ab1) (e.g., resistance to western maize rootworm, northern maize rootworm, and Mexican maize rootworm); Yield Gard VT Root worm / RR2 maize with glyphosate tolerance and resistance to maize rootworm; and Bollgard 3 cotton (registered trademark) XtendFlex (registered trademark) with dicamba tolerance, glyphosate tolerance, glufosinate tolerance and resistance to a lepidopteran pest (e.g., resistance to ball caterpillars, tobacco hornworms, fall armyworms and similar pests).

[00120] In the present method, the type of nozzle to be used when compound X and trolamine salt 2,4-D are applied can be a flat fan nozzle or a drift-reducing nozzle. Examples of flat fan nozzles include the Teejet 110 and XR Teejet 110 series products manufactured by Teejet. The volumetric average diameter of the liquid droplets ejected through each nozzle, when used at a normal spray pressure, generally from 30 (2.10 kg / cm2) to 120 PSI (8.43 kg / cm2), is generally less than 430 microns. A drift-reducing nozzle is a nozzle with reduced drift compared to a flat fan nozzle and is called an air-induction nozzle or pre-orifice nozzle. The volumetric average diameter of the liquid droplets ejected through the drift-reducing nozzle is generally 430 microns or more.

[00121] An air induction nozzle has an air guide between the inlet (spray liquid introduction part) and the outlet (spray liquid ejection part), allowing the formation of air-filled liquid droplets when mixing the spray liquid. Petition 870250084601, dated 09 / 19 / 2025, page 36 / 98 30 / 76 Air induction. Examples of air induction nozzles include: TDXL11003-D, TDXL11004-D1, TDXL11005-D1 and TDXL11006-D, manufactured by Green Leaf Technology; TTI110025, TTI11003, TTI11004, TTI11005, TTI11006 and TTI11008, manufactured by Teejet; and ULD120-041, ULD120-051 and ULD120-061, manufactured by Pentair. A particularly preferable model is the TTI11004.

[00122] A pre-orifice nozzle is a nozzle in which an inlet (a part where the spray liquid is introduced) serves as a metering orifice, so that large droplets of liquid can be formed by controlling the amount of flow to be injected into the nozzle, thus decreasing the pressure in the nozzle. When a pre-orifice nozzle is used, the pressure during spray liquid ejection can be reduced by half compared to the pressure before spray liquid introduction. Examples of pre-orifice nozzles include: DR110-10, UR110-05, UR110-06, UR110-08 and UR110-10, manufactured by Wilger; and Turf Jet 1 / 4TTJ08 and Turf Jet 1 / 4TTJ04, manufactured by Teejet.

[0079]

[00123] The sprayer used to apply compound X and 2,4-D trolamine salt in the present method may be a hooded sprayer certified by the U.S. Environmental Protection Agency (EPA) as drift reduction technology (DRT). Examples of DRT-certified hooded sprayers include REDBALL 642, REDBALL 642E, REDBALL SPK645, REDBALL 645, REDBALL 645T, REDBALL SP645, REDBALL ATV642 and similar models from Willmar Fabrication LLC.

[00124] When the present method is carried out in a cultivated field, such as a crop field, compound X and the 2,4-D trolamine salt can be applied before sowing, and compound X and the 2,4-D trolamine salt can be applied simultaneously and / or after sowing. Petition 870250084601, dated 09 / 19 / 2025, page 37 / 98 31 / 76

[00125] That is, compound X and the 2,4-D trolamine salt are applied once before, simultaneously with, or after sowing the crop seeds; twice, except before sowing the crop seeds, twice, except simultaneously with sowing the crop seeds, or twice, except after sowing the crop seeds; or three times before, simultaneously with, and after sowing the crop seeds.

[00126] When compound X and the 2,4-D trolamine salt are applied before sowing the crop seeds, compound X and the 2,4-D trolamine salt are generally applied 50 days before sowing or immediately before sowing, preferably 30 days before sowing or immediately before sowing, more preferably 20 days before sowing or immediately before sowing, and even more preferably 10 days before sowing or immediately before sowing.

[00127] When compound X and 2,4-D trolamine salt are applied after sowing the seeds, compound X and 2,4-D trolamine salt are generally applied immediately after sowing or before flowering, preferably immediately after sowing and before emergence, or between the 1st and 6th true leaf stages of the seeds.

[00128] When compound X and 2,4-D trolamine salt are applied simultaneously with seed sowing, the machine to be used is a machine in which the seeder and spraying equipment are integrated.

[00129] Examples of the method for applying compound X and 2,4-D trolamine salt include a soil application for applying compound X and 2,4-D trolamine salt to the soil where weeds will grow, and a foliar application for applying to weeds that are growing and are developing. When compound X and salt Petition 870250084601, dated 09 / 19 / 2025, p. 38 / 98 32 / 76 of 2,4-D trolamine are applied to weeds during growth; the treatment can be a foliar application and a soil application. The timing of the treatment can be decided depending on the growth stage of the aforementioned crops; for example, foliar application for weeds can be carried out before sowing the crops, or soil application for weeds can be carried out during crop growth. These treatments can be applied uniformly across the soil or as a localized treatment.

[00130] In the present method, localized treatment is the opposite concept to uniform, comprehensive herbicide treatment, and means selectively applying the herbicide to the location where weeds are growing or will grow. The term "localized application" means an application to weeds or soil if weeds are growing, or application to soil where weeds are expected to occur in the future if weeds have not yet grown. The case where compound X and 2,4-D trolamine salt are lightly sprayed on-site by spreading or transpiration where weeds are not growing or may not grow in the future is also included in localized treatment, unless it is a uniform coverage treatment. Only the case where all locations where weeds are growing or will grow are selectively treated in a continuous crop field is not considered localized treatment.In other words, even if a portion of the cultivated field is treated comprehensively, or if a portion of the area where weeds are growing or will grow is not treated, the treatment is included in the localized treatment if there is a treated area within the continuous cultivated field. Localized treatment can be carried out by avoiding specific crops or it can be applied based solely on the location. Petition 870250084601, dated 09 / 19 / 2025, page 39 / 98 33 / 76 weed control, regardless of crop location.

[00131] Specific examples of the localized treatment method are shown below. Localized treatment can be carried out as follows: in the crop field, a sprayer visually applies compound X and 2,4-D trolamine salt using a hand-held nozzle or a robotic arm nozzle while walking or riding on a mobile device or flying device. Localized treatment can also be carried out by previously mapping the location where weeds are growing or may grow and applying compound X and 2,4-D trolamine salt based on the map information.In map-based applications, localized treatment can be performed, in addition to the method mentioned above, by automatically or manually opening and closing the nozzle of the boom or robotic arm while the spraying equipment is in operation or in flight, based on the spraying equipment's location information (obtained by GPS) and map information. Map information can be created based on image information obtained by a manned or unmanned flying object, or map information can be created visually by an observer walking on the ground, an observer in a device operating on the ground, or an observer in a flying device.Furthermore, the spraying equipment in operation or in flight has the function of detecting the location where weeds are growing or will grow, and localized treatment can be carried out by the boom or robotic arm during real-time mapping. These technologies are mentioned in Patent Documents (e.g., WO 2018001893, WO 2018036909) and Non-Patent Documents (e.g., Crop Protection 26, 270-277, Weed Technology 17, 711-717, Applied Engineering). Petition 870250084601, dated 09 / 19 / 2025, page 40 / 98 34 / 76 ring in Agriculture 30, 143-152). These technologies correspond to an emerging form of agriculture called precision agriculture, smart agriculture, or digital agriculture, and the non-uniform spraying style generated by localized treatment is also called variable rate application (VRA) as the term in emerging agriculture. The technology in which the operating spraying equipment performs localized treatment in real time while detecting growing weeds is also known as 'See & Spray' or optical localized spraying (OSST) technology.

[00132] The location where weeds will grow can be estimated based on whether they formed a patch of vegetation in the previous growing season, or it can be estimated from the distribution of buried seeds. The distribution of buried seeds can be examined by soil sampling or by remote sensing.

[00133] The present method can exert a synergistic herbicidal effect on a wide range of weeds, compared to the effect expected from the effects obtained when each of the compounds X and the 2,4-D trolamine salt are applied separately, and can also efficiently control a wide range of weeds in a cultivated field and in a vegetable garden, where normal cultivation is carried out with or without tillage, an orchard and an uncultivated area, without producing harmful effects that could be a problem for useful plants.

[00134] In the present method, it is possible to use it in combination with other agrochemical active ingredients, for example, insecticides, nematicides and fungicides. Examples of such insecticides, nematicides and fungicides include neonicotinoid-based compounds, diamide-based compounds, carbamate-based compounds, compounds based Petition 870250084601, dated 09 / 19 / 2025, page 41 / 98 35 / 76 organophosphates, biological nematicides and other insecticides, as well as azole-based compounds, strobilurin-based compounds, metalaxyl-based compounds, SDHI compounds, other fungicides and plant growth regulators.

[00135] Specific examples of weeds that can be controlled by the present compound or by the present method include, but are not limited to, the following weeds.

[00136] Urticaceae weeds: small nettle (Urtica urens)

[00137] Polygonaceae weeds: black creeper (Polygonum convolvulus), pale persicaria (Polygonum lapathifolium), Pennsylvania creeper (Polygonum pensylvanicum), red cinnamon (Polygonum persicaria), longisetum, aviculare creeper (Polygonum aviculare), arenastrum creeper (Polygonum arenastrum), Japanese mistletoe (Polygonum cuspidatum), Japanese dock (Rumex japonicus), curly dock (Rumex crispus), blunt-leaved dock (Rumex obtusifolius), sorrel (Rumex acetose)

[00138] Weeds Portulacaceae: common purslane (Portulaca oleracea)

[00139] Weeds Caryophyllaceae: Stellaria media, Stellaria aquatica, Cerastium holosteoides, Cerastium glomeratum, Spergula arvensis, Silene gallica

[00140] Molluginaceae weeds: carpet weed (Mollugo verticillata)

[00141] Chenopodiaceae weeds: common quarterle (Chenopodium album), Indian goosefoot (Chenopodium ambrosioides), kochia (Bassia scoparia), prickly salga (Salsola kali), orach (Atriplex spp.) Petition 870250084601, dated 09 / 19 / 2025, page 42 / 98 36 / 76

[00142] Weeds of Amaranthaceae: red amaranth (Amaranthus retroflexus), slender amaranth (Amaranthus viridis), livid amaranth (Amaranthus lividus), spiny amaranth (Amaranthus spinosus), smooth amaranth (Amaranthus hybridus), Palmer amaranth (Amaranthus palmen), green amaranth (Amaranthus patulus), hemp (Amaranthus tuberculatus = Amaranthus rudis = Amaranthus tamariscinus), prostrate amaranth (Amaranthus blitoides), large-fruited amaranth (Amaranthus deflexus), mucronated amaranth (Amaranthus quitensis), alligator weed (Alternanthera philoxeroides), sessile alligator weed (Alternanthera sessilis), perrofolha (Alternanthera tenella)

[00143] Papaveraceae weeds: common poppy (Papaver rhoeas), field poppy (Papaver dubium), Mexican prickly poppy (Argemone mexicana)

[00144] Brassicaceae weeds: wild radish (Raphanus raphanistrum), radish (Raphanus sativus), wild mustard (Sinapis arvensis), shepherd's purse (Capsella bursa-pastoris), white mustard (Brassica juncea), rapeseed (Brassica napus), pinnate tansy mustard (Descurainia pinnata), yellow swamp cress (Rorippa islandica), yellow cress (Rorippa sylvestris), field cress (Thlaspi arvense), turnip weed (Myagrum rugosum), Virginia pepperweed (Lepidium virginicum), slender cress (Coronopus didymus)

[00145] Weeds Capparaceae: African cabbage (Cleome affinis)

[00146] Fabaceae weeds: Indian vetch (Aeschynomene indica), zigzag vetch (Aeschynomene rudis), hemp sesbania (Sesbania exaltata), sicklepod (Cassia obtusifolia), coffee senna (Cassia occidentalis), Florida beggar weed (Desmodium tortuosum), wild peanut (Desmodium adscendens), Illinois tick clover (Desmodium illinoense), clover Petition 870250084601, dated 09 / 19 / 2025, p. 43 / 98 37 / 76 white (Trifolium repens), kudzu (Pueraria lobata), narrow-leaf vetch (Vicia angustifolia), hairy indigo (Indigofera hirsuta), Indigofera truxillensis, common cowpea (Vigna sinensis)

[00147] Weeds Oxalidaceae: creeping sorrel (Oxalis corniculata), European sorrel (Oxalis stricta), red clover (Oxalis oxyptera)

[00148] Weeds Geraniaceae: Carolina geranium (Geranium carolinense), blind beak (Erodium cicutarium)

[00149] Weeds Euphorbiaceae: Euphorbia helioscopia, Euphorbia maculata, Euphorbia humistrata, Euphorbia esula, wild poinsettia (Euphorbia heterophylla), hyssop (Euphorbia brasiliensis), turmeric (Acalypha australis), tropical croton (Croton glandulosus), lobed croton (Croton lobatus), phyllanthus corcovadensis, castor bean (Ricinus communis)

[00150] Malvaceae weeds: velvet leaf (Abutilon theophrasti), arrow leaf Sida (Sida rhombifolia), heart leaf Sida (Sida cordifolia), prickly Sida (Sida spinosa), Sida glaziovii, Sida santaremnensis, bladder weed (Hibiscus trionum), spore-bearding Anoda (Anoda cristata), false mallow with spiny seeds (Malvastrum coromandelianum)

[00151] Weeds Onagraceae: Ludwigia epilobioides, long-fruited primrose willow (Ludwigia octovalvis), winged primrose (Ludwigia decurrens), common primrose (Oenothera biennis), cut-leaf primrose (Oenothera laciniata)

[00152] Weeds of Sterculiaceae: Florida waltheria (Waltheria indica)

[00153] Weeds Violaceae: field violet; Viola arvensis, wild violet; Viola tricolor

[00154] Weeds Cucurbitaceae: slender-beaked cucumber (Sicyos angulatus), wild cucumber (Echinocystis lobata), balsam apple Petition 870250084601, dated 09 / 19 / 2025, page 44 / 98 38 / 76 amargosa (Momordica charantia)

[00155] Weeds Lythraceae: Ammannia multiflora, slender-beaked herb (Ammannia auriculata), scarlet calyx (Ammannia coccinea), purple salicyria (Lythrum salicaria), Indian calyx (Rotala indica)

[00156] Weeds Elatinaceae: three-stamen watercress (Elatine triandra), California watercress (Elatine californica)

[00157] Apiaceae weeds: Chinese celery (Oenanthe javanica), wild carrot (Daucus carota), carrot fern (Conium maculatum)

[00158] Weeds Araliaceae: lawn pennywort (Hydrocotyle sibthorpioides), floating pennywort (Hydrocotyle ranunculoides)

[00159] Weeds Ceratophyllaceae: common hornbill (Ceratophyllum demersum)

[00160] Weeds of Cabombaceae: Carolina fanwort (Cabomba caroliniana)

[00161] Haloragaceae weeds: Brazilian aquatic yarrow (Myriophyllum aquaticum), Spiral aquatic yarrow (Myriophyllum verticillatum), Aquatic yarrow (Myriophyllum spicatum, Myriophyllum heterophyllum, etc.)

[00162] Sapindaceae weeds: heartseed (Cardiospermum halicacabum)

[00163] Primulaceae weeds: scarlet pimpernel (Anagallis arvensis)

[00164] Asclepiadaceae weeds: common milkweed (Asclepias syriaca), honey milkweed (Ampelamus albidus)

[00165] Rubiaceae weeds: bedding straw (Galium aparine), Galium spurium var. echinospermon, broadleaf buttonweed (Spermacoce latifolia), Brazilian calla lily (Richardia brasiliensis), broadleaf buttonweed (Borreria alata) Petition 870250084601, dated 09 / 19 / 2025, page 45 / 98 39 / 76

[00166] Weeds of the Convolvulaceae family: Ipomoea nill, Ipomoea hederacea, Ipomoea purpurea, Ipomoea hederacea var. integriuscula, Ipomoea lacunosa, Ipomoea trilobada (Ipomoea triloba), Ipomoea acuminata, Ipomoea hederifolia, Ipomoea coccinea, Ipomoea quamoclit, Ipomoea grandifolia, Ipomoea aristolochiaefolia, Ipomoea cairica, red field creeper (Convolvulus arvensis), Japanese creeper (Calystegia hederacea), Japanese creeper (Calystegia japonica), ivy creeper (Merremia hederacea), hairy creeper (Merremia aegyptia), road creeper (Merremia cissoides), small-flowered morning glory (Jacquemontia tamnifolia)

[00167] Weeds Boraginaceae: field forget-me-not (Myosotis arvensis)

[00168] Weeds of Lamiaceae: purple nettle (Lamium purpureum), common hen weed (Lamium amplexicaule), lion's ear (Leonotis nepetaefolia), wild spikenard (Hyptis suaveolens), Hyptis lophanta, Siberian mothergrass (Leonurus sibiricus), nettle betony (Stachys arvensis)

[00169] Solanaceae weeds: jimsonweed (Datura stramonium), black belladonna (Solanum nigrum), American black belladonna (Solanum americanum), oriental black belladonna (Solanum ptycanthum), hairy belladonna (Solanum sarrachoides), buffalo borer (Solanum rostratum), soda apple belladonna (Solanum aculeatissimum), sticky belladonna (Solanum sisymbriifolium), Indian nettle (Solanum carolinense), cut-leaf pitanga (Physalis angulata), smooth pitanga (Physalis subglabrata), Peruvian apple (Nicandra physalodes)

[00170] Weeds of the Scrophulariaceae family: veronica (Veronica hederaefolia), veronica (Veronica persica), veronica arvensis, lilinderia procumbens, lilinderia dubia, lildernia angustifolia, round-leaved water hyssop (Bacopa rotundifolia), Petition 870250084601, dated 09 / 19 / 2025, page 46 / 98 40 / 76 dopatrium (Dopatrium junceum), Gratiola japonica

[00171] Plantaginaceae: Asian plantain (Plantago asiatica), narrow-leaved plantain (Plantago lanceolata), broad-leaved plantain (Plantago major), marsh star (Callitriche palustris)

[00172] Weeds Asteraceae: common burdock (Xanthium pensylvanicum), large burdock (Xanthium occidentale), Canadian burdock (Xanthium italicum), common sunflower (Helianthus annuus), wild chamomile (Matricaria chamomilla), scentless chamomile (Matricaria perforata), corn marigold (Chrysanthemum segetum), ray chamomile (Matricaria matricarioides), Japanese mugwort (Artemisia princeps), common mugwort (Artemisia vulgaris), Chinese mugwort (Artemisia verlotorum), tall goldenrod (Solidago altissima), common dandelion (Taraxacum officinale), hairy galinsoga (Galinsoga ciliata), small-flowered galinsoga (Galinsoga parviflora), common rubber tree (Senecio vulgaris), soul's flower (Senecio brasiliensis), Senecio grisebachii, horseweed (Conyza bonariensis),Guernsey fleabane (Conyza sumatrensis), common ragweed (Conyza canadensis), common ragweed (Ambrosia artemisiifolia), giant ragweed (Ambrosia trifida), three-slit marigold (Bidens tripartita), hairy blackjack (Bidens pilosa), common blackjack (Bidens frondosa), greater blackjack (Bidens subalternans), Canadian thistle (Cirsium arvense), black thistle (Cirsium vulgare), milk thistle (Silybum marianum), musk thistle (Carduus nutans), prickly lettuce (Lactuca serriola), annual sowthis (Sonchus oleraceus), prickly sowthis (Sonchus asper), beach blackwood (Wedelia glauca), perfoliate blackwood (Melampodium perfoliatum), red tassel (Emilia sonchifolia), wild marigold (Tagetes minuta), watercress (Blainvillea latifolia), coat buttons (Tridax procumbens), Bolivian coriander (Porophyllum ruderale), Paraguayan starbur (Acanthospermum australe), bristly starbur (Acanthospermum hispidum), balloon creeper (Cardiospermum hali, Petition 870250084601, dated 09 / 19 / 2025, page 47 / 98 41 / 76 cacabum), tropic ageratum (Ageratum conyzoides), common spinewort (Eupatorium perfoliatum), fireweed (Erechtites hieracifolia), American fennel (Gamochaeta spicata), linear-leaved fennel (Gnaphalium spicatum), Jaegeria hirta, parthenium ragweed (Parthenium hysterophorus), little yellow crown (Siegesbeckia orientalis), lawn Soliva sessilis, Eclipta prostrata, Eclipta alba, Centipeda minima

[00173] Alismataceae weeds: dwarf arrowhead (Sagittaria pygmaea), three-leafed arrowhead (Sagittaria trifolia), arrowhead (Sagittaria sagittifolia), giant arrowhead (Sagittaria montevidensis), Sagittaria aginashi, piped water banana (Alisma canaliculatum), common water banana (Alisma plantagoaquatica)

[00174] Weeds of Limnocharitaceae: Flowering rush Sawah (Limnocharis flava)

[00175] Hydrocharitaceae weeds: American toad (Limnobium spongia), Florida elodea (Hydrilla verticillata), common water nymph (Najas guadalupensis)

[00176] Araceae weeds: Nile cabbage (Pistia stratiotes)

[00177] Weeds Lemnaceae: three-veined duckweed (Lemna aoukikusa, Lemna paucicostata, Lemna aequinoctialis), common duck meat (Spirodela poliriza), Wolffia spp. Weeds Potamogetonaceae: roundleaf algae (Potamogeton distinct), weeds (Potamogeton crispus, Potamogeton illinoensis, Stuckenia pectinata, etc.)

[00178] Weeds of Liliaceae: wild onion (Allium canadensis), wild garlic (Allium vineale), Chinese garlic (Allium macrostemon)

[00179] Pontederiaceae weeds: common water hyacinth (Eichhornia) Petition 870250084601, dated 09 / 19 / 2025, page 48 / 98 42 / 76 hornia crassipes), blue mud banana (Heteranthera limosa), Monochoria korsakowii, heart-shaped false mistletoe (Monochoria vaginalis)

[00180] Weeds Commelinaceae: common dayflower (Commelina communis), tropical spiderweed (Commelina benghalensis), erect dayflower (Commelina erecta), Asian spiderweed (Murdannia keisak)

[00181] Poaceae weeds: common barnyardgrass (Echinochloa crus-galli), early barnyardgrass (Echinochloa oryzicola), barnyardgrass (Echinochloa crus-galli var. formosensis), late watergrass (Echinochloa oryzoides), jungle rice (Echinochloa colonum), Gulf spur (Echinochloa crus-pavonis), green foxtail (Setaria viridis), giant foxtail (Setaria faberi), yellow foxtail (Setaria glauca), knotted foxtail (Setaria geniculata), southern crabgrass (Digitaria ciliaris), large crabgrass (Digitaria sanguinalis), Jamaican crabgrass (Digitaria horizontalis), yellow grass (Digitaria insularis), goosegrass (Eleusine indica), annual bluegrass (Poa annua), rough-stalked meadowgrass (Poa trivialis), Kentucky bluegrass (Poa pratensis), short-winged foxgrass (Alopecurus aequalis), blackgrass (Alopecurus myosuroides), wild oat (Avena fatua), Johnson grass (Sorghum halepense), shataken (grain sorghum;Sorghum vulgare), guackgrass (Agropyron repens), Italian ryegrass (Lolium multiflorum), perennial ryegrass (Lolium perenne), bomugi (rigid ryegrass; Lolium rigidum), rescue brome (Bromus catharticus), shaggy brome (Bromus tectorum), Japanese brome grass (Bromus japonicus), trickery grass (Bromus secalinus), trickery grass (Bromus tectorum), foxtail barley (Hordeum jubatum), articulated goat grass (Aegilops cylindrica), canary grass (Phalaris arundinacea), small-seeded canary grass (Phalaris minor), curved and silky grass (Apera; Petition 870250084601, dated 09 / 19 / 2025, p. 49 / 98 43 / 76 spica-venti), autumn panicum (Panicum dichotomiflorum), Texas panicum (Panicum texanum), Guinea grass (Panicum maximum), broadleaf signal grass (Brachiaria platyphylla), Congo signal grass (Brachiaria ruziziensis), Alexander grass (Brachiaria plantaginea), Suriname grass (Brachiaria decumbens), palisade grass (Brachiaria brizantha), creeping signal grass (Brachiaria humidicola), southern burr (Cenchrus echinatus), field burr (Cenchrus pauciflorus), woolly grass (Eriochloa villosa), feathered pennisetum (Pennisetum setosum), Rhodes grass (Chloris gayana), Rhodes grass (Chloris virgata), Indian grass (Eragrostis pilosa), Natal grass (Rhynchelytrum repens), crow grass (Dactyloctenium aegyptium), swamp grass (Ischaemum rugosum), swamp millet (Isachne globosa), common rice (Oryza sativa), Bahia grass (Paspalum notatum), coastal sand grass (Paspalum maritimum), Mercer grass (Paspalum distichum), Kikuyu grass (Pennisetum clandestinum),West Indian pennisetum (Pennisetum setosum), itchy grass (Rottboellia cochinchinensis), Asian prairie grass (Leptochloa chinensis), salt meadow grass (Leptochloa fascicularis), Christmas tree grass (Leptochloa filiformis), Amazonian prairie grass (Leptochloa panicoides), Japanese cutting grass (Leersia japonica), Leersia sayanuka, cutting grass (Leersia oryzoides), Glyceria leptorrhiza, sharp-scaled manna grass (Glyceria acutiflora), large aquatic grass (Glyceria maxima), redtop (Agrostis gigantea), folded carpet (Agrostis stolonifera), Bermuda grass (Cynodon) dactylon), cocksfoot (Dactylis glomerata), centipede grass (Eremochloa ophiuroides), tall fescue (Festuca arundinacea), red fescue (Festuca rubra), lalang (Imperata cylindrica), Chinese fairy grass (Miscanthus sinensis), foxtail grass (Panicum virgatum), Japanese lawn grass (Zoysia japonica),

[00182] Weeds Cyperaceae: Cyperus microiria, Cyperus Petition 870250084601, dated 09 / 19 / 2025, page 50 / 98 44 / 76 would, Cyperus compressus, Cyperus difformis, Cyperus flaccidus, Cyperus globosus, Cyperus nipponicus, Cyperus odoratus, Cyperus serotinus, Cyperus rotundus, Cyperus esculentus, Kyllinga gracillima, Kyllinga brevifolia, Fimbristylis miliacea, Fimbristylis dichotoma, Eleocharis acicularis, Eleocharis kuroguwai, Japanese sedge (Schoenoplectiella hotarui), hard-stemmed sedge (Schoenoplectiella juncoides), Schoenoplectiella wallichii, rough-seeded sedge (Schoenoplectiella mucronatus), Schoenoplectiella triangulatus, Schoenoplectiella nipponicus, triangular sedge (Schoenoplectiella triqueter), Bolboschoenus koshevnikovii, river rush (Bolboschoenus fluviatilis)

[00183] Weeds of the Equisetaceae family: field horsetail (Equisetum arvense), marsh horsetail (Equisetum palustre)

[00184] Weeds of the Salviniaceae family: floating fern (Salvinia natans)

[00185] Weeds of the Azollaceae family: Japanese fern (Azolla japonica), pinnate fern (Azolla pinnata)

[00186] Weeds of the Marsileaceae family: bracken fern (Marsilea quadrifolia)

[00187] Others: fibrous algae (Pithophora, Cladophora), Bryophyta, Marchantiophyta, Anthocerotophyta, Cyanobacteria, Pteridophyta, shoots of perennial crops (pome fruits, stone fruits, berries, nuts, citrus fruits, hops, grapes, etc.)

[00188] In the weeds above, mutations within the species are not particularly limited. That is, weeds also include any weeds that have reduced sensitivity (also called resistance) to a specific herbicide. Reduced sensitivity may be attributed to a mutation at a target site (target-site mutation) or may be attributed to any other factors besides the mutation at the target site (non-target-site mutation). The mu Petition 870250084601, dated 09 / 19 / 2025, p. 51 / 98 45 / 76 Target site mutations include those in which the substitution of an amino acid residue of a protein as a target site occurred due to a mutation in an open reading frame corresponding to the amino acid sequence of the protein, and those in which the protein as a target site is overexpressed due to mutations such as deletion of the suppressor sequence in the promoter region, amplification of the enhancer sequence, or an increase in the number of gene copies.

[00189] Examples of factors that reduce sensitivity due to non-target site mutation include increased metabolism, defective absorption, defective transition, efflux out of the system, and the like. Examples of increased metabolism include increased activity of metabolic enzymes such as cytochrome P450 (CYP) monooxygenases, aryl acylamidases (AAA), esterases, and glutathione transansferase (GST). Efflux out of the system includes transport to a vacuole by an ABC transporter.

[00190] Examples of herbicide-resistant weeds include the following: Glyphosate Resistance:

[00191] Examples of reduced weed sensitivity due to target site mutation include weeds in which the substitution of any amino acid residue or multiple amino acid residues selected from among the amino acid residues mentioned below occurs in the EPSPS gene. Thr102Ile, Pro106Ser, Pro106Ala, Pro106Leu, and Pro106Thr. In particular, those with Thr102Ile and Pro106Ser, and those with Thr102Ile and Pro106Thr are mentioned. Indian goosegrass (Eleusine indica), Italian ryegrass (Lolium multiflorum), rigid ryegrass (Lolium rigidum), perennial ryegrass (Lolium perenne), Bidens subalternans, and similar weeds, all resistant to glyphosate, can be effectively controlled. Similarly, examples of glyphosate resistance where the target site Petition 870250084601, dated 09 / 19 / 2025, p. 52 / 98 46 / 76 target is involved, including those in which the copy number of the EPSPS gene is increased (PNAS, 2018 115 (13) 3332-3337). According to the present invention, glyphosate-resistant Amaranthus palmeri, Amaranthus tuberculatus, Kochia (Bassia scoparia) and similar weeds in which the copy number of the EPSPS gene is increased can be effectively controlled. Examples of reduced weed sensitivity due to non-target site mutation include glyphosate-resistant horseweed (Conyza canadensis), Sumatran horseweed (Conyza sumatrensis), flax fleabane (Conyza bonariensis) and similar weeds in which an ABC transporter is involved can be effectively controlled according to the present invention. Furthermore, wild rice (Echinochloa colona), in which glyphosate sensitivity is reduced by increased aldo-keto reductase expression, known as non-target site mutation (Plant Physiology 181, 1519-1534), is effectively controlled by the present invention. Resistance to ALS-inhibiting herbicides:

[00192] Examples of reduced weed sensitivity due to target site mutation include weeds, each with a mutation capable of causing the substitution of one or more amino acid residues selected from among the amino acid residues mentioned below in the ALS gene as a target site mutation. Ala122Thr, Ala122Val, Ala122Tyr, Pro197Ser, Pro197His, Pro197Thr, Pro197Arg, Pro197Leu, Pro197Gln, Pro197Ala, Pro197Ile, Ala205Val, Ala205Phe, Asp376Glu, Asp376Gln, Asp376Asn, Arg377His, Trp574Leu, Trp574Gly, Trp574Met, Ser653Thr, Ser653Asn, Ser635Ile, Gly654Glu and Gly645Asp. According to the present invention, red-root amaranth (Amaranthus retroflexus), smooth amaranth (Amaranthus hybridus), Palmer amaranth (Amaranthus palmen), water amaranth (Amaranthus tuberculatus), kochia (Bassia scoparia) and similar plants, resistant to ALS inhibitors, can be treated with alkali-resistant compound. Petition 870250084601, dated 09 / 19 / 2025, p. 53 / 98 47 / 76 effectively controlled. Examples of reduced weed sensitivity due to non-target site mutation include weeds that possess a non-target site mutation involving CYP or GST to make them resistant to an ALS inhibitor, and these weeds can also be effectively controlled according to the present invention. Known examples of weeds include stiff ryegrass (Lolium rigidum), in which CYP81A10 and CYP81A1v1 are overexpressed, barnyardgrass (Echinochloa oryzoides), in which CYP81A12 and CYP81A21 are overexpressed, and blackgrass (Alopecurus myosuroides Huds), in which GSTF1 and GSTU2 are overexpressed. Resistance to ACCase inhibitors:

[00193] Examples of reduced weed sensitivity due to target site mutation include weeds that each have a mutation capable of causing the substitution of one or more amino acid residues in the ACCase gene. Ile1781Ala, Ile1781Leu, Ile1781Val, Ile1781Thr, Leu1818Phe, Trp1999Cys, Trp1999Leu, Trp1999Ser, Ala2004Val, Trp2027Cys, Trp2027Leu, Ile2041Asn, Ile2041Asp, Ile2041Val, Ile2041Thr, Asp2078Gly, Asp2078Glu, Cys2088Arg, Cys2088Phe, Gly2096Ala, Gly2096Ser. According to the present invention, ACCase-resistant weeds that have these mutations at the target site are effectively controlled. Similarly, examples of resistance to ACCase inhibitors in which the target site is involved include those in which ACCase gene expression is increased (Pest Manag. Sci. 2017 Nov;73(11):2227-2235). Examples of reduced weed sensitivity due to mutations at non-target sites include weeds that become resistant to an ACCase inhibitor as a result of CYP or GST involvement. Examples of weeds include rigid ryegrass (Lolium rigidum), in Petition 870250084601, dated 09 / 19 / 2025, p. 54 / 98 48 / 76 in which CYP81A10 and CYP81A1v1 are overexpressed, barnyardgrass (Echinochloa oryzoides), in which CYP81A12 and CYP81A21 are overexpressed, and blackgrass (Alopecurus myosuroides Huds), in which GSTF1 and GSTU2 are overexpressed. Resistance to PPO inhibitors:

[00194] Examples of reduced weed sensitivity due to target site mutation include weeds that each have a mutation capable of causing the substitution of one or more amino acid residues in the PPO gene. These mutations are known as carfentrazone-ethyl, fomesafen, and lactofen resistance mutations, or are predicted to become carfentrazone-ethyl, fomesafen, and lactofen resistance mutations. Arg128Leu, Arg128Met, Arg128Gly, Arg128His, Arg128Ala, Arg128Cys, Arg128Glu, Arg128Ile, Arg128Lys, Arg128Asn, Arg128Gln, Arg128Ser, Arg128Thr, Arg128Val, Arg128Tyr, Gly210 deficiency, Ala210 deficiency, Gly210Thr, Ala210Thr, Gly211 deficiency, Gly114Glu, Ser149Ile, Val361Ala, Gly399Ala (all amino acid numbers are standardized by the PPO2 sequence of Palmer amaranth (Amaranthus palmen)). Typically, the PPO genes in a weed include the PPO1 and PPO2 genes. The mutation mentioned above can occur in one or both of the PPO1 and PPO2 genes.It is preferable that the mutation occurs in the PPO2 gene. For example, Arg128Met means that a mutation occurs at an amino acid residue located at position 128. In the PPO2 gene of common ragweed (Ambrosia artemisiifolia), the mutation corresponds to position 98 (Weed Science 60, 335-344) and is known as Arg98Leu, this Arg98 being the same as Arg128 in the present descriptive report. In the PPO gene of weeds to be controlled by the present invention, Arg128Met and Arg128Gly are known in peregrine amaranth (Amaranthus palmeri) (Pest Management Science 73, 1559-1563), Arg128Gly is known as PPO2 of câ. Petition 870250084601, dated 09 / 19 / 2025, p. 55 / 98 49 / 76 aquatic hemp (Amaranthus tuberculatus) (Pest Management Science, 2019; 75: 3235-3244), Arg128Ile and Arg128Lys are known as the PPO2 of aquatic hemp (Amaranthus tuberculatus) (Pest Management Science, 2019; 75: 3235-3244), Arg128His is known as Arg132His for the PPO2 of rigid ryegrass (Lolium rigidum) (WSSA Annual Meeting, 2018), Gly114Glu, Ser149Ile, Val361Ala and Gly399Ala are known for the PPO2 of Palmer amaranth (Amaranthus palmeri) (Frontiers in Plant Science 10, Article 568, and Plants 2023, 12(9), 1886), and Ala210Thr is known as Ala212Thr by PPO1 from Indian goosegrass (Eleusine indica) (Pest Management Science, doi: 10.1002 / ps.5703). According to the present invention, weeds resistant to PPO inhibitors, each with one of the mutations in the target site mentioned above, can be effectively controlled. However, the weed resistant to PPO inhibitors to be controlled is not limited to these weeds.That is, not only Palmer amaranth (Amaranthus palmeri) with a mutation of Arg128Leu, Arg128Met, Arg128Gly, Arg128His, Arg128Ala, Arg128Cys, Arg128Glu, Arg128Ile, Arg128Lys, Arg128Asn, Arg128Gln, Arg128Ser, Arg128Thr, Arg128Val, Arg128Tyr, Gly210 deficiency, Ala210 deficiency, Gly210Thr, Ala210Thr, Gly211 deficiency, Gly114Glu, Ser149Ile or Gly399Ala in PPO1 or PPO2, but also, for example, water amaranth (Amaranthus tuberculatus) with the same mutation, Common ragweed (Ambrosia artemisiifolia) with the same mutation and wild poinsettia (Euphorbia cyathophora) with the same mutation can be effectively controlled. As an example of reduced weed sensitivity due to mutation at the non-target site, amaranthus tuberculatus, which becomes resistant to carfentrazonaethyl, is known as amaranthus tuberculatus, and amarantopalmeri, which becomes resistant to a PPO inhibitor due to CYP or GST involvement (PLOS ONE, doi:). Petition 870250084601, dated 09 / 19 / 2025, page 56 / 98 50 / 76 10.1371 / joumal.pone.0215431), these weeds being effectively controlled according to the present invention. Resistance to auxin-type herbicides:

[00195] Examples of target site mutations include a mutation that causes Gly-Asn in a degron region in the AUX / IAA gene. According to the present invention, kochia (Bassia scoparia), palmer amaranth (Amaranthus palmer), and water amaranth (Amaranthus tuberculatus), each with this mutation, can be effectively controlled. As non-target site mutations, dicamba-resistant green amaranth (Amaranthus chlorostachys Will) and 2,4-D-resistant water amaranth (Amaranthus tuberculatus) are known, in which CYP involvement is suggested. These weeds can be effectively controlled according to the present invention. These weeds can also be controlled in the case of the non-target site mutation in which GST is involved. Resistance to HPPD inhibitors:

[00196] Examples of reduced weed sensitivity due to non-target site mutation include water amaranth (Amaranthus tuberculatus), Palmer amaranth (Amaranthus palmeri), and similar plants, each of which becomes resistant to an HPPD inhibitor as a result of the involvement of CYP or GST, which are effectively controlled according to the present invention. As examples of this, Palmer amaranth (Amaranthus palmeri) is known, in which CYP72A219, CYP81B, and CYP81E8 are overexpressed. Resistance to photosystem II inhibitors:

[00197] Examples of reduced weed sensitivity due to target site mutation include weeds, each with a mutation capable of causing the substitution of one or more amino acid residues selected from among the amino acid residues mentioned below in the psbA gene. Val219Ile, Ser264Gly, Ser264Ala, Petition 870250084601, dated 09 / 19 / 2025, p. 57 / 98 51 / 76 Phe274Val. According to the present invention, Palmer amaranth (Amaranthus palmer) and seagrass (Amaranthus tuberculatus), resistant to photosystem II inhibitors, each with this mutation at the target site, can be effectively controlled. Examples of reduced weed sensitivity due to non-target site mutation include Palmer amaranth (Amaranthus palmer), seagrass (Amaranthus tuberculatus), and similar weeds, each of which becomes resistant to a photosystem II inhibitor as a result of the involvement of CYP, GST, or AAA. These weeds can be effectively controlled according to the present invention. As an example, rigid ryegrass (Lolium rigidum), in which CYP71R4 is overexpressed, is known. Resistance to glutamate synthase inhibitors:

[00198] Examples of reduced weed sensitivity due to target site mutation include weeds possessing a mutation capable of causing the Asp171Asn amino acid substitution in a glutamate synthase gene. According to the present invention, Palmer amaranth (Amaranthus palmeri), water amaranth (Amaranthus tuberculatus), and similar weeds resistant to glutamate synthase inhibitors, each with this target site mutation, can be effectively controlled. Examples of reduced weed sensitivity due to non-target site mutation include Palmer amaranth (Amaranthus palmeri), water amaranth (Amaranthus tuberculatus), and similar weeds, each of which becomes resistant to glufosinate as a result of CYP or GST involvement. These weeds can be effectively controlled according to the present invention. As an example, palmer amaranth (Amaranthus palmer) is known to overexpress CYP72A219, CYP81B, and CYP81E8.

[00199] Resistant weeds, each with a combination Petition 870250084601, dated 09 / 19 / 2025, page 58 / 98 52 / 76 A stack of at least two groups selected from the groups mentioned above (two arbitrarily selected groups, three arbitrarily selected groups, four arbitrarily selected groups, five arbitrarily selected groups, six arbitrarily selected groups, seven arbitrarily selected groups, or eight arbitrarily selected groups) can also be effectively controlled. For example, water amaranth (Amaranthus tuberculatus) is known to exhibit resistance to all of the following: photosystem II inhibitor, HPPD inhibitor, 2,4-D, PPO inhibitor, ALS inhibitor, and glyphosate. This weed can also be effectively controlled. The stack can be a combination of mutations at the target site, or a combination of mutations at the non-target site, or a combination of one mutation at the target site and one mutation at the non-target site.

[00200] Examples of herbicides that may be included in the present composition, in addition to compound X and the 2,4-D trolamine salt, or herbicides that may be used in combination with compound X and the 2,4-D trolamine salt in the present method include the following herbicides. These herbicides may also be used in combination with the present composition, which comprises only compound X and the 2,4-D trolamine salt as active ingredients.

[00201] Glyphosate and a salt thereof (isopropylammonium salt, ammonium salt, potassium salt, guanidine salt, dimethylamine salt, monoethanolamine salt, choline salt, BAPMA salt (N,N-bis(aminopropyl)methylamine), 2,4-D and a salt or ester thereof (ammonium salt, butyl ester, 2-butoxypropyl ester, butyl ester, diethylammonium salt, dimethylammonium salt, diolamine salt, dodecylammonium salt, ethyl ester, 2-ethylhexyl ester, heptylammonium salt, isobutyl ester, isooctyl ester, isopropyl ester, isopropylammonium salt, lithium salt, meptyl ester, methyl ester, octyl ester, pentyl ester, Petition 870250084601, dated 09 / 19 / 2025, page 59 / 98 53 / 76 propyl ester, sodium salt, tefuryl ester, tetradecylammonium salt, triethylammonium salt, tris(2-hydroxypropyl)ammonium salt, choline salt), 2,4DB and a salt or ester thereof (dimethylammonium salt, iso-octyl ester, choline salt), pyroxasulfone, dicamba and a salt or ester thereof (diglycolamine salt, dimethylammonium salt, diolamine salt, isopropylammonium salt, methyl ester, auramine salt, potassium salt, sodium salt, trolamine salt, BAPMA salt (N,N-bis(aminopropyl)methylamine), choline salt, TBA salt (tetrabutylammonium), TBP salt (tetrabutylphosphonium), MCPA and a salt or ester thereof (dimethylammonium salt, 2-ethylhexyl ester, ester of iso-octyl, sodium salt, choline salt), MCPB, mecoprop and a salt or an ester thereof (dimethylammonium salt, diolamine salt, ethadyl ester, 2-ethylhexyl ester, iso-octyl ester, methyl ester, potassium salt, sodium salt, trolamine salt, choline salt),mecoprop-P and a salt or ester thereof (dimethylammonium salt, 2-ethylhexyl ester, isobutyl salt, potassium salt, choline salt), dichlorprop and a salt or ester thereof (butyl ester, dimethylammonium salt, 2-ethylhexyl ester, iso-octyl ester, methyl ester, potassium salt, sodium salt, choline salt), dichlorprop-P, dichlorprop-P-dimethylammonium, quinclorac, quinmerac, bromoxynil, bromoxynil-octanoate, diclobenyl, methiazolinone, ioxynil, ioxynil-octanoate, dilate, butylate, trialate, fenemedifame, chlorprofame, desmedifame, asulam, fenisofame, bentiocarb, molinate, esprocarb, piributicarb, prosulfocarb, orbencarb, EPTC, dimepiperate, swep, propachlor, metazachlor, alachlor, acetochlor, metolachlor, S-metolachlor, butachlor, pretylachlor, tenylchlor, aminocyclopyrachlor, aminocyclopyrachlor-methyl, aminocyclopyrachlor-potassium, trifluralin, pendimethalin, etalfluralin, benfluralin, prodiamine, simazine, atrazine, propazine, cyanazine, ametrine, symethrin,dimethamethrin, promethrin, indaziflam, icafolin-methyl, triaziflam, metribuzin, hexazinone, terbumetone, terbuthylazine, terbutrine, triethazine, isoxaben, diuron, Petition 870250084601, dated 09 / 19 / 2025, page 60 / 98 54 / 76 linurone, metobromurone, methoxurone, monolinurone, sidurone, fluometurone, difenoxurone, methyl-daimurone, isoproturone, isourone, tebutiurone, benzothiazurone, metabenzthiazurone, propanil, mefenacet, clomeprope, naproanilide, bromobutide, daimurone, cumilurone, diflufenzopyr, etobenzanide, bentazone, tridiphane, indanophane, amitrol, fenchlorazole, clomazone, maleic hydrazide, pyridate, chloridazone, norflurazone, bromacil, terbacil, lenacil, oxaziclomephone, cinmethylin, benfuresate, cafenstrol, flufenacet, piritiobac, piritiobac-sodium, piriminobac, piriminobac-methyl, bispiribac, bispiribac-sodium, piribenzoxim, pirimisulfan, pirifalide, triafamonium, fentrazamide, dimethenamide, dimethenamide-P, ACN, dithiopyr, triclopyr and a salt or ester thereof (butyl ester, triethylammonium salt), fluroxypyr, fluroxypyr-meptyl, thiazopyr, aminopyralid and a salt thereof (potassium salt, triisopropanolammonium salt, choline salt), clopyralid and a salt thereof (olamine salt,potassium salt, triethylammonium salt, choline salt), picloram and a salt thereof (potassium salt, triisopropanolammonium salt, choline salt), dalapon, chlorthiamide, amidosulfurone, azinsulfurone, bensulfurone, bensulfurone-methyl, chlorimurone, chlorimurone-ethyl, cyclosulfamurone, ethoxysulfurone, flazasulfurone, flucetosulfurone, flupyrsulfurone, flupyrsulfurone-methyl-sodium, foransulfurone, halosulfurone, halosulfurone-methyl, imazosulfurone, mesosulfurone, mesosulfurone-methyl, metazosulfurone, nicosulfurone, orthosulfamurone, oxasulfurone, primisulfurone, primisulfurone-methyl, propyrissulfurone, pyrazosulfurone, pyrazosulfurone-ethyl, rinsulfurone, sulfometurone, sulfometurone-methyl, sulfosulfurone, trifloxysulfurone-sodium, trifloxysulfurone, chlorsulfurone, cinosulfurone, etametsulfurone, etametsulfurone-methyl, iodosulfurone, iodosulfurone-methyl-sodium, iofensulfurone, iofensulfurone-sodium, metsulfurone, metsulfurone-methyl, prosulfurone, tifensulfuronetifensulfurone-methyl, triasulfurone, tribenurone, tribenurone-methyl, triflusulfurone, triflus, Petition 870250084601, dated 09 / 19 / 2025, page 61 / 98 55 / 76 sulfone-metila, tritosulfurone, diflufenicon, picolinafeno, beflubutamide, norflurazone, fluridone, flurocloridone, flurtamone, rimisoxafene, benzobiciclone, biciclopyrone, mesotrione, sulcotrione, tefuriltrione, tembotrione, isoxaclortol, isoxaflutol, benzofenap, pirasulfotol, pirazolinato, pirazoxifene, topramezone, tolpiralate, lancotrione-sodium, flupoxam, amicarbazone, bencarbazone, flucarbazone, flucarbazone-sodium, ipfencarbazone, propoxicarbazone, propoxicarbazone-sodium, tiencarbazone, tiencarbazone-methyl, cloransulam, cloransulam-methyl, diclosulam, florasulam, flumetsulam, metosulam, penoxsulam, piroxsulam, imazametabenz, imazametabenz-methyl, imazamox, imazamoxamonium, imazapic, imazapic-ammonium, imazapyr, imazapyr-ammonium, imazaquin, imazaquin-ammonium, imazethapyr, imazethapyr-ammonium, clodinafop, clodinafop-propargyl, cihalofop, cihalofop-butyl, diclofop, diclofop-methyl, fenoxaprop, fenoxaprop-ethyl, fenoxaprop-P, fenoxaprop-P-ethyl, fluazifop, fluazifop-butylfluazifop-P, fluazifop-P-butyl, haloxyfop, haloxyfop-methyl, haloxyfop-P, haloxyfop-P-methyl, metamifop, propaquizafop, quizalofop, quizalofop-ethyl, quizalofop-P, quizalofop-P-ethyl, aloxidim, clethodim, sethoxydim, tepraloxidim, tralkoxidim, pinoxaden, methoproxibicyclone, fenoxasulfone, glufosinate, glufosinate-ammonium, glufosinate-P, glufosinate-P-sodium, bialafos, anilophos, bensulide, butamifos, paraquat, paraquat-dichloride, diquat, diquate-dibromide, halauxifen, halauxifen-methyl, florpyrauxifen, florpyrauxifen-benzyl, flumiclorac-pentyl, fomesafen-sodium, lactophen, acifluorfen-sodium, acloniphene, bifenox, clomethoxyphene, chlornitrophene, ethoxyphene-ethyl, fluorodifene, fluoroglycophene-ethyl, fluoronitrophene, halosaphene, nitrophene, nitrofluorfene, oxyfluorfene, cinidone-ethyl, profluazole, pyraclonil, oxadiargil, oxadiazone, pentoxazone, fluazolate, piraflufene-ethyl, benzfendizone, butaphenacil, fluthiacetomethyl, thidiazimine, azaphenidine, carfentrazone-ethyl,sulfentrazone, flufenpyr-ethyl, indolauxipyr or a salt thereof, indolauxipyr-cyanomethyl, bixlozone, cyclopyranil, phenquinotrione, cyclopyranil. Petition 870250084601, dated 19 / 09 / 2025, p. 62 / 98. 56 / 76 rimorate, tetflupirolimet, dimesulfazete and iptriazopyride.

[00202] In the present invention, particularly preferred examples of herbicides that can be used in combination with compound X and the 2,4-D trolamine salt are: glyphosate-potassium salt, glyphosate-guanidine salt, glyphosate-dimethylamine salt, glyphosate-monoethanolamine salt, glufosinate-ammonium salt, glyphosate-isopropylammonium salt, diflufenican, rimisoxafen, icafolin-methyl, piroxasulfone, dicamba diglycolamine salt, dicamba BAPMA salt, dicamba TBA salt, dicamba TBP salt, flumiclorac-pentyl, clethodim, metproxibicyclone, lactofen, S-metolachlor, metribuzin, flufenacet, nicosulfurone, rimsulfurone, acetochlor, mesotrione, isoxaflutol, chlorimuron-ethyl, tifensulfuron-methyl, chloransulam-methyl and imazethapyrammonium salt.

[00203] In the present invention, the ratio of the herbicide that can be used in combination with compound X and the 2,4-D trolamine salt to compound X is generally within a range of 0.01 to 1,000 times by weight, and preferably 0.1 to 300 times by weight. In this descriptive report, when the active ingredient of the herbicides is a salt (e.g., glyphosate-potassium salt, glyphosate-monoethanolamine salt), its weight means an acid equivalent, unless otherwise specified.

[00204] Examples of specific combinations that are more preferable when using compound X and 2,4-D trolamine salt in combination with one or more herbicides include compound X + 2,4-D trolamine salt + glyphosate-potassium salt, and compound X + 2,4-D trolamine salt + glyphosate-monoethanolamine salt.

[00205] In cultivating a crop in the present invention, plant nutritional management can be carried out in a common crop. A fertilization system can be based on Precision Agriculture or it can be a conventional homogeneous system. Alternatively, a Petition 870250084601, dated 09 / 19 / 2025, page 63 / 98 57 / 76 A nitrogen-fixing bacterium or a mycorrhizal fungus can be inoculated along with a seed treatment. [Examples]

[00206] The present invention will now be described in more detail by means of Examples. However, the present invention is not limited to these Examples.

[00207] First, the criteria for evaluating the herbicidal effect and the harmful effect on crops demonstrated in the examples mentioned below will be described. [Herbicidal effect and harmful effect on crops]

[00208] The herbicidal effect is classified on a scale of 0 to 100, where 0 is a classification in which the emergence or growth status of a sample weed during a test shows no difference or shows little difference compared to the status of the test weed that was not subjected to treatment, and 100 is a classification in which the test plant is completely killed or the emergence or growth of the test weed is completely suppressed.

[00209] The harmful effect on crops is classified as harmless when little harmful effect is observed, low when a moderate level of harmful effect is observed, medium when a medium level of harmful effect is observed, and high when a high level of harmful effect is observed. Example 1

[00210] The weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faben) are sown in a plastic pot filled with soil. On the same day, 70 g / ha of flumioxazin + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface with Petition 870250084601, dated 09 / 19 / 2025, page 64 / 98 58 / 76 a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 7 days, soybeans are sown, and after 14 days, the herbicidal effect on weeds and crop damage in soybeans are investigated. The synergistic effect in weed control due to the combined use of flumioxazin and 2,4-D trolamine salt is confirmed. Example 2

[00211] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, common ragweed, common barnyardgrass, Echinochloa crus-galli, and giant foxtail, Setaria faben) and soybeans are sown in a plastic pot filled with soil. On the same day, 140 g / ha of flumioxazin + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 21 days, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect on weed control due to the combined use of flumioxazin and 2,4-D trolamine salt is confirmed. Example 3

[00212] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application of 70 g / ha of flumioxazin + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, 14 days after treatment, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect in weed control due to the combined use of flumioxazin and 2,4-D trolamine salt is confirmed. Petition 870250084601, dated 09 / 19 / 2025, pp. 65 / 98 59 / 76 Example 4

[00213] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) are sown in a plastic pot with soil. On the same day, 12.5 g / ha of trifludimoxazine + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface with a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 7 days, soybeans are sown, and after 14 days, the herbicidal effect on weeds and damage to the soybean crop are investigated. The synergistic effect in weed control due to the combined use of trifludimoxazine and 2,4-D trolamine salt is confirmed. Example 5

[00214] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Echinochloa crus-galli, and Setaria faben) and soybeans were sown in a plastic pot filled with soil. On the same day, 25 g / ha of trifludimoxazine + 600 and 900 or 1200 g / ha of 2,4-D trolamine salt were sprayed onto the soil surface at a spray volume of 200 L / ha. The plants were then grown in a greenhouse, and after 21 days, the effect on weeds and damage to the soybean crop was investigated. The synergistic effect on weed control due to the combined use of trifludimoxazine and 2,4-D trolamine salt was confirmed. Example 6

[00215] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application with 12.5 g / ha of tri Petition 870250084601, dated 09 / 19 / 2025, pp. 66 / 98 60 / 76 g fludimoxazine + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt, at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 14 days of treatment, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect on weed control due to the combined use of trifludimoxazine and 2,4-D trolamine salt is confirmed. Example 7

[00216] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli, and Setaria faben) are sown in a plastic pot filled with soil. On the same day, 25 g / ha of saflufenacil + 600 and 900 or 1200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface with a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 7 days, soybeans are sown. After 14 days, the herbicidal effect on weeds and crop damage in soybeans are investigated. The synergistic effect on weed control due to the combined use of saflufenacil and 2,4-D trolamine salt is confirmed. Example 8

[00217] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Echinochloa crus-galli, and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. On the same day, 50 g / ha of saflufenacil + 600 and 900 or 1200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 21 days, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect on weed control due to the combined use of saflufenacil and 2,4-D trolamine salt is confirmed. Petition 870250084601, dated 09 / 19 / 2025, pp. 67 / 98 61 / 76 Example 9

[00218] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) and soybeans are sown in a plastic pot with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application with 25 g / ha of saflufenacil + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 14 days of treatment, the effect on weeds and damage to the soybean crop are investigated. The synergistic effect in weed control due to the combined use of saflufenacil and 2,4-D trolamine salt is confirmed. Example 10

[00219] The weeds (palmer amaranth, water amaranth, tuber amaranth, common ragweed, giant ragweed, giant ragweed, trifid ragweed, Canadian fleabane, Conyza canadensis, sheepskin, Chenopodium album, Kochia scoparia, barnyardgrass, Echinochloa crus-galli and giant foxtail, Setaria faberi) are sown in a plastic pot filled with soil. On the same day, 25 g / ha of thiafenacil + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 7 days, soybeans are sown. After 14 days, the herbicidal effect on weeds and damage to the soybean crop are investigated. The synergistic effect on weed control due to the combined use of thiafenacil and 2,4-D trolamine salt is confirmed. Example 11

[00220] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Echinochloa crus-galli and Setaria Petition 870250084601, dated 09 / 19 / 2025, pp. 68 / 98 62 / 76 faben) and soybeans are sown in a plastic pot filled with soil. On the same day, 50 g / ha of thiafenacil + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 21 days, the effect on weeds and crop damage in soybeans is investigated. The synergistic effect on weed control due to the combined use of thiafenacil and 2,4-D trolamine salt is confirmed. Example 12

[00221] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faben) and soybeans are sown in a plastic pot filled with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application with 25 g / ha of thiafenacil + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt, at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 14 days of treatment, the effect on weeds and damage to the soybean crop are investigated. The synergistic effect in weed control due to the combined use of thiafenacil and 2,4-D trolamine salt is confirmed. Example 13

[00222] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) are sown in a plastic pot with soil. On the same day, 25 g / ha of flufenoxymacyl + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface with a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 7 days, soybeans are sown, and after 14 days, the herbicide effect is complete. Petition 870250084601, dated 09 / 19 / 2025, page 69 / 98 The effects of 63 / 76 on weeds and crop damage in soybeans are investigated. The synergistic effect on weed control due to the combined use of flufenoxymacyl and 2,4-D trolamine salt is confirmed. Example 14

[00223] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, common ragweed, common barnyardgrass, Echinochloa crus-galli, and giant foxtail, Setaria faben) and soybeans are sown in a plastic pot filled with soil. On the same day, 50 g / ha of flufenoxymacyl + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 21 days, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect on weed control due to the combined use of flufenoxymacyl and 2,4-D trolamine salt is confirmed. Example 15

[00224] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application of 25 g / ha of flufenoxymacyl + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 14 days of treatment, the effect on weeds and damage to the soybean crop are investigated. The synergistic effect in weed control due to the combined use of flufenoxymacyl and 2,4-D trolamine salt is confirmed. Example 16

[00225] Weeds (Amaranthus palmeri, Amaranthus tuber Petition 870250084601, dated 09 / 19 / 2025, pp. 70 / 98 64 / 76 *Culatus*, common ragweed, giant ragweed, trifida ragweed, Canadian fleabane, *Conyza canadensis*, *Chenopodium album*, *Kochia scoparia*, common barnyardgrass, *Echinochloa crus-galli*, and giant foxtail, *Setaria faben*) are sown in a plastic pot filled with soil. On the same day, 25 g / ha of compound 1 + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 7 days, soybeans are sown. After 14 days, the herbicidal effect on weeds and damage to the soybean crop are investigated. The synergistic effect on weed control due to the combined use of compound 1 and 2,4-D trolamine salt is confirmed. Example 17

[00226] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Echinochloa crus-galli, and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. On the same day, 50 g / ha of compound 1 + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 21 days, the effect on weeds and crop damage in soybeans is investigated. The synergistic effect on weed control due to the combined use of compound 1 and 2,4-D trolamine salt is confirmed. Example 18

[00227] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application with 25 g / ha of Petition 870250084601, dated 09 / 19 / 2025, page 71 / 98 65 / 76 compound 1 + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 14 days of treatment, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect on weed control due to the combined use of compound 1 and 2,4-D trolamine salt is confirmed. Example 19

[00228] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, common ragweed, giant ragweed, trifida ragweed, Conyza canadensis, common swamp grass, Chenopodium album, Kochia scoparia, common barnyardgrass, Echinochloa crus-galli, and giant foxtail, Setaria faben) are sown in a plastic pot filled with soil. On the same day, 25 g / ha of compound 2 + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 7 days, soybeans are sown, and after 14 days, the herbicidal effect on weeds and damage to the soybean crop are investigated. The synergistic effect in weed control due to the combined use of compound 2 and the 2,4-D trolamine salt is confirmed. Example 20

[00229] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Echinochloa crus-galli, and Setaria faberi) and soybeans are sown in a plastic pot filled with soil. On the same day, 50 g / ha of compound 2 + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L / ha. The plants are then grown in a greenhouse, and after 21 days, the effect on weeds and crop damage in soybeans is investigated. The synergistic effect on weed control due to the combined use of compound 2 and salt is observed. Petition 870250084601, dated 09 / 19 / 2025, pp. 72 / 98 66 / 76 of 2,4-D trolamine is confirmed. Example 21

[00230] Weeds (Amaranthus palmeri, Amaranthus tuberculatus, Ambrosia artemisiifolia, Ambrosia trifida, Conyza canadensis, Chenopodium album, Bassia scoparia, Echinochloa crus-galli and Setaria faben) and soybeans are sown in a plastic pot filled with soil. The plants are then grown in a greenhouse and, 21 days after sowing, are subjected to a foliar application with 25 g / ha of compound 2 + 600 and 900 or 1,200 g / ha of 2,4-D trolamine salt at a spray volume of 200 L / ha. The plants are then grown in a greenhouse and, after 14 days of treatment, the effect on weeds and damage to the soybean crop is investigated. The synergistic effect in weed control due to the combined use of compound 2 and the 2,4-D trolamine salt is confirmed. Examples 22 to 42

[00231] In each of Examples 1 to 21, Roundup WeatherMax (660 g / L of glyphosate-potassium salt, manufactured by Monsanto Company) is added to the spray liquid containing compound X and 2,4-D trolamine salt, so that the application rate is 2.338 L / ha (32 fluid ounces / acre), followed by application in the same manner. Examples 43 to 63

[00232] In each of Examples 22 to 42, XtendiMax (350 g / L of dicamba diglycolamine salt, in terms of dicamba acid, manufactured by Monsanto Company) is added to the spray liquid containing compound X and the 2,4-D trolamine salt, so that the application rate is 1,607 ml / ha (22 fluid ounces / acre), followed by application in the same manner. Examples 64 to 84

[00233] In each of Examples 1 to 21, the RoundupExtend (240 Petition 870250084601, dated 09 / 19 / 2025, p. 73 / 98 67 / 76 g / L of glyphosate monoethanolamine + 120 g / L of dicamba diglycolamine (manufactured by Monsanto Company) is added to the spray liquid containing compound X and the 2,4-D trolamine salt, so that the application rate is 4.677 l / ha (64 fluid ounces / acre), followed by application in the same manner. Examples 85 to 168

[00234] The application is carried out in the same way, except that the soybeans from Examples 1 to 84 are replaced by corn or cotton. Example 169

[00235] NipsIt (600 g / L clothianidin, manufactured by Valent USA LLC) is applied to soybean seeds (variety: Genuity Roundup Ready2Yield soybean) so that the application rate of NipsIt can be 206 mL / kg of seeds (1.28 fluid ounces / 100 nursery seeds). The spray liquid obtained by mixing the formulation containing Valor SX (510 g / kg flumioxazin, manufactured by Valent USA LLC) and the 2,4-D trolamine salt (hereinafter referred to as formulation Y) with water is sprayed onto the agricultural field before soybean sowing, so that the application rate of flumioxazin can be 70, 105 or 140 g / ha and the application rate of the 2,4-D trolamine salt can be 600, 900 or 1,200 g / ha. Seven days after spraying, the soybeans are sown in the field.At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is sprayed onto the field, so that the application rate can be 2,338 L / ha (32 fluid ounces / acre). Example 170

[00236] NipsIt (600 g / L clothianidin, manufactured by Valent USA LLC) is applied to soybean seeds (variety: Genuity Roundup Ready2Yield soybean) such that the application rate of NipsIt is 206 mL / kg of seeds (1.28 fluid ounces / 100 nursery seeds). Petition 870250084601, dated 09 / 19 / 2025, p. 74 / 98 68 / 76 The spray solution obtained by mixing Vulcarus (415.3 g / kg trifludimoxazin, manufactured by BASF Corporation) and formulation Y with water is sprayed onto the field before soybean sowing, so that the application rate of trifludimoxazin can be 12.5 or 25 g / ha and the application rate of 2,4-D trolamine salt can be 600, 900, or 1200 g / ha. Seven days after spraying, the soybeans are sown in the field. At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L glyphosate-potassium salt, manufactured by Monsanto Company) is sprayed onto the field, so that the application rate is 2.338 L / ha (32 fluid ounces / acre). Example 171

[00237] NipsIt (600 g / L clothianidin, manufactured by Valent USA LLC) is applied to soybean seeds (variety: Genuity Roundup Ready2Yield soybean) so that the application rate of NipsIt is 206 mL / kg of seeds (1.28 fluid ounces / 100 nursery seeds). The spray liquid obtained by mixing Sharpen (297.4 g / kg saflufenacil, manufactured by BASF Corporation) and formulation Y with water is sprayed onto the field before soybean sowing, so that the saflufenacil application rate can be 25 or 50 g / ha and the 2,4-D trolamine salt application rate can be 600, 900, or 1200 g / ha. Seven days after spraying, the soybeans are sown in the field. At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is sprayed onto the field, so that the application rate can be 2,338 L / ha (32 fluid ounces / acre). Example 172

[00238] NipsIt (600 g / L clothianidin, manufactured by Valent USA LLC) is applied to soybean seeds (variety: Genuity Round soybean). Petition 870250084601, dated 09 / 19 / 2025, p. 75 / 98 69 / 76 dup Ready2Yield) so that the application rate of NipsIt is 206 mL / kg of seeds (1.28 fluid ounces / 100 nursery seeds). The spray liquid obtained by mixing Gamma (700 g / kg of thiafenacil, manufactured by Helm) and formulation Y with water is sprayed on the field before soybean sowing, so that the application rate of trifludimoxazin can be 25 or 50 g / ha and the application rate of 2,4-D trolamine salt can be 600, 900 or 1,200 g / ha. Seven days after spraying, the soybeans are sown in the field. At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is sprayed onto the field so that the application rate is 2,338 L / ha (32 fluid ounces / acre). Example 173

[00239] NipsIt is applied to soybean seeds in the same manner as in Example 169. Value SX, formulation Y, and RoundupWeatherMax are applied to the field before soybean sowing, so that the flumioxazin application rate can be 70 or 140 g / ha, the 2,4-D trolamine salt application rate can be 600, 900, or 1200 g / ha, and the RoundupWeatherMax application rate (660 g / L glyphosate-potassium salt, manufactured by Monsanto Company) can be 2.338 L / ha (32 fluid ounces / acre). After 7 days, the soybeans are sown in the field. At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field at an application rate of 2,338 L / ha (32 fluid ounces / acre). Example 174

[00240] NipsIt is applied to soybean seeds in the same manner as in Example 169, followed by sowing in the field. The day after sowing, the SX Value and Y formulation are applied to the field so that the flumioxazin application rate is Petition 870250084601, dated 09 / 19 / 2025, p. 76 / 98 70 / 76 of 70 or 140 g / ha and the application rate of 2,4-D trolamine salt is 600, 900 or 1,200 g / ha. At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field so that the application rate is 2,338 L / ha (32 fluid ounces / acre). Example 175

[00241] NipsIt is applied to soybean seeds in the same manner as in Example 169, followed by sowing in the field. The day after sowing, Valor SX, formulation Y, and RoundupWeatherMax are applied to the field, such that the flumioxazin application rate is 70 or 140 g / ha, the 2,4-D trolamine salt application rate is 600, 900, or 1,200 g / ha, and the RoundupWeatherMax application rate (660 g / L glyphosate / potassium salt, manufactured by Monsanto Company) is 2,338 L / ha (32 fluid ounces / acre). At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field at an application rate of 2,338 L / ha (32 fluid ounces / acre). Example 176

[00242] NipsIt is applied to soybean seeds in the same manner as in Example 170. Vulcarus, formulation Y, and RoundupWeatherMax are applied to the field before soybean sowing, so that the application rate of trifludimoxazin can be 12.5 or 25 g / ha, the application rate of 2,4-D trolamine salt can be 600, 900, or 1200 g / ha, and the application rate of RoundupWeatherMax (660 g / L glyphosate-potassium salt, manufactured by Monsanto Company) can be 2.338 L / ha (32 fluid ounces / acre), and after 7 days, the soybean is sown in the field. At the three true leaf stage of the soybean, RoundupWeatherMax (660 g / L glyphosate salt) is applied. Petition 870250084601, dated 09 / 19 / 2025, p. 77 / 98 71 / 76 sate / potassium, manufactured by Monsanto Company) is applied in the agricultural field so that the application rate can be 2,338 L / ha (32 fluid ounces / acre). Example 177

[00243] NipsIt is applied to soybean seeds in the same manner as in Example 170, followed by sowing in the field. The day after sowing, Vulcarus and formulation Y are applied to the field so that the application rate of trifludimoxazin can be 12.5 or 25 g / ha and the application rate of 2,4-D trolamine salt can be 600, 900, or 1,200 g / ha. At the three true leaf stage of soybeans, Roundup WeatherMax (660 g / L glyphosate-potassium salt, manufactured by Monsanto Company) is applied to the field so that the application rate can be 2,338 L / ha (32 fluid ounces / acre). Example 178

[00244] NipsIt is applied to soybean seeds in the same manner as in Example 170, followed by sowing in the field. The day after sowing, Vulcarus, formulation Y, and RoundupWeatherMax are applied to the field, such that the application rate of trifludimoxazin is 12.5 or 25 g / ha, the application rate of 2,4-D trolamine salt is 600, 900, or 1,200 g / ha, and the application rate of RoundupWeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is 2.338 L / ha (32 fluid ounces / acre). At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field at an application rate of 2,338 L / ha (32 fluid ounces / acre). Example 179

[00245] NipsIt is applied to soybean seeds in the same manner as in Example 171. Sharpen, formulation Y and RoundupWeatherPetition 870250084601, dated 09 / 19 / 2025, p. 78 / 98 72 / 76 Max products are applied to the field before soybean sowing, so that the application rate of saflufenacil can be 25 or 50 g / ha, the application rate of 2,4-D trolamine salt can be 600, 900 or 1,200 g / ha, and the application rate of Roundup Weather Max (660 g / L of glyphosate-potassium salt, manufactured by Monsanto Company) can be 2.338 L / ha (32 fluid ounces / acre), and after 7 days, the soybean is sown in the field. At the three true leaf stage of the soybean, Roundup Weather Max (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field, so that the application rate is 2.338 L / ha (32 fluid ounces / acre). Example 180

[00246] NipsIt is applied to soybean seeds in the same manner as in Example 171, followed by sowing in the field. The day after sowing, Sharpen and formulation Y are applied to the field, so that the application rate of saflufenacil can be 25 or 50 g / ha and the application rate of 2,4-D trolamine salt can be 600, 900 or 1,200 g / ha. At the three true leaf stage of soybeans, Roundup WeatherMax (660 g / L glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field, so that the application rate is 2,338 L / ha (32 fluid ounces / acre). Example 181

[00247] NipsIt is applied to soybean seeds in the same manner as in Example 171, followed by sowing in the agricultural area. The day after sowing, Sharpen, formulation Y, and Roundup WeatherMax are applied to the crop, such that the saflufenacil application rate is 25 or 50 g / ha, the 2,4-D trolamine salt application rate is 600, 900, or 1200 g / ha, and the Roundup WeatherMax application rate (660 g / L of glyphosate-potassium salt, manufactured) Petition 870250084601, dated 09 / 19 / 2025, p. 79 / 98 73 / 76 by Monsanto Company) is 2,338 L / ha (32 fluid ounces / acre). At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate-potassium salt, manufactured by Monsanto Company) is applied to the crop, so that the application rate is 2,338 L / ha (32 fluid ounces / acre). Example 182

[00248] NipsIt is applied to soybean seeds in the same manner as in Example 172. Gamma, formulation Y, and Roundup WeatherMax are applied to the field before soybean sowing, so that the application rate of thiafenacil can be 25 or 50 g / ha, the application rate of 2,4-D trolamine salt can be 600, 900, or 1,200 g / ha, and the application rate of Roundup WeatherMax (660 g / L of glyphosate-potassium salt, manufactured by Monsanto Company) can be 2.338 L / ha (32 fluid ounces / acre). After 7 days, the soybeans are sown in the field. At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field at an application rate of 2,338 L / ha (32 fluid ounces / acre). Example 183

[00249] NipsIt is applied to soybean seeds in the same manner as in Example 172, followed by sowing in the field. The day after sowing, Gamma and formulation Y are applied to the field, so that the application rate of thiafenacil can be 25 or 50 g / ha and the application rate of 2,4-D trolamine salt can be 600, 900 or 1,200 g / ha. At the three true leaf stage of soybeans, Roundup WeatherMax (660 g / L glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field, so that the application rate is 2,338 L / ha (32 fluid ounces / acre). Example 184 Petition 870250084601, dated 09 / 19 / 2025, pp. 80 / 98 74 / 76

[00250] NipsIt is applied to soybean seeds in the same manner as in Example 172, followed by sowing in the field. The day after sowing, Gamma, formulation Y, and Roundup WeatherMax are applied to the field, such that the application rate of thiafenacil is 25 or 50 g / ha, the application rate of 2,4-D trolamine salt is 600, 900, or 1,200 g / ha, and the application rate of Roundup WeatherMax (660 g / L glyphosate / potassium salt, manufactured by Monsanto Company) is 2,338 L / ha (32 fluid ounces / acre). At the three-true-leaf stage of soybeans, Roundup WeatherMax (660 g / L of glyphosate / potassium salt, manufactured by Monsanto Company) is applied to the field at an application rate of 2,338 L / ha (32 fluid ounces / acre). Examples 185 to 200

[00251] In each of Examples 169 to 184, when applying Roundup WeatherMax the day after sowing or at the three true leaf stage of soybeans, XtendiMax is added to Roundup WeatherMax so that the application rate of XtendiMax (350 g / L of dicamba diglycolamine salt in terms of dicamba acid, manufactured by Monsanto Company) can be 1,607 ml / ha (22 fluid ounces / acre), followed by application. Examples 201 to 216

[00252] In each of Examples 169 to 184, when applying Roundup WeatherMax the day after sowing or at the three true leaf stage of soybeans, Roundup Extend (240 g / L glyphosate monoethanolamine + 120 g / L dicamba diglycolamine, manufactured by Monsanto Company) is used in place of Roundup WeatherMax and applied so that the application rate of Roundup Extend is 4.677 L / ha (64 fluid ounces / acre). Examples 217 to 264

[00253] In each of Examples 169 to 216, INOVATE (160 g / L Petition 870250084601, dated 09 / 19 / 2025, p. 81 / 98 75 / 76 clothianidin + 13 g / L metalaxyl + 8 g / L ipconazole, manufactured by Valent USA) is used in place of NipsIt, and is applied so that the application rate of INOVATE is 309 mL / 100 kg of seeds (4.74 fluid ounces / 100 nursery seeds). Examples 265 to 312

[00254] In each of Examples 169 to 216, CruiserMAXX Vibrance (240 g / L thiamethoxam + 36 g / L metalaxyl M + 12 g / L fludioxonil + 12 g / L sedaxane, manufactured by Syngenta Ltd.) is used in place of NipsIt and applied in such a way that the application rate of CruiserMAXX Vibrance can be 235 mL / 100 kg of seeds (3.22 fluid ounces / 100 nursery seeds). Examples 313 to 360

[00255] In each of Examples 169 to 216, the Acceleron system (DX-612 (326 g / L fluxapyroxad, manufactured by Monsanto Company) 31 ml / 100 kg of seeds + DX-309 (313 g / L metalaxyl, manufactured by Monsanto Company), 242 ml / 100 kg of seeds (1.5 fluid ounces / 100 pond seeds) + DX-109 (200 g / L pyraclostrobin, manufactured by Monsanto Company) 242 ml / 100 kg of seeds (1.5 fluid ounces / 100 pond seeds) + IX-104 (600 g / L imidacloprid, manufactured by Monsanto Company) 515 ml / 100 kg of seeds (3.2 fluid ounces / 100 pond seeds)) is applied instead of the NipsIt application for seeds of soy. Examples 361 to 552

[00256] In each of Examples 169 to 360, corn or cotton seeds are used in place of soybean seeds. Examples 553 to 936

[00257] In each of Examples 169 to 552, the application is carried out in the same way, except that the crop to be grown is replaced by crops with the Roundup Ready 2 Xtend characteristic. Examples 937 to 1320 Petition 870250084601, dated 09 / 19 / 2025, pp. 82 / 98 76 / 76

[00258] In each of Examples 169 to 552, the application is carried out in the same manner, except that the crop to be grown is replaced by crops with the Roundup Ready 2 Xtend trait and a trait tolerant to a PPO inhibitor. Examples 1321 to 1704

[00259] In each of Examples 169 to 552, the application is carried out in the same manner, except that the crop to be grown is replaced by crops with the Roundup Ready 2 Xtend trait, a trait tolerant to a PPO inhibitor, and a trait tolerant to an HPPD inhibitor. [Industrial Applicability]

[00260] According to the present invention, it is possible to control weeds with high effectiveness. Petition 870250084601, dated 09 / 19 / 2025, pp. 83 / 98

Claims

1 / 5 CLAIMS 1. Herbicidal composition, characterized in that it comprises at least one compound selected from the following group of compounds X and a salt of 2,4-D trolamine: Group of compounds X: group consisting of flumioxazin, trifludimoxazin, saflufenacil, thiafenacil, flufenoximacyl, a compound represented by the following formula (I): and a compound represented by the following formula (II): 0 (H).

2. Herbicidal composition, according to claim 1, characterized in that the weight ratio of at least one compound selected from the group of compounds X to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:1 to 1:

100.

3. Herbicidal composition, according to claim 1, characterized in that at least one compound selected from the group of compounds X is flumioxazin.

4. Herbicidal composition, according to claim 3, characterized in that the weight ratio of flumioxazin to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:5 to 1:

20.

5. Herbicidal composition, according to claim 1, Petition 870250084601, dated 09 / 19 / 2025, page 84 / 98 2 / 5, characterized in that at least one compound selected from the group of compounds X is trifludimoxazine.

6. Herbicidal composition, according to claim 5, characterized in that the weight ratio of trifludimoxazine to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:

100.

7. Herbicidal composition, according to claim 1, characterized in that at least one compound selected from the group of compounds X is saflufenacil.

8. Herbicidal composition, according to claim 7, characterized in that the weight ratio of saflufenacil to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:

50.

9. Herbicidal composition, according to claim 1, characterized in that at least one compound selected from the group of compounds X is thiaphenacil.

10. Herbicidal composition, according to claim 9, characterized in that the weight ratio of thiaphenacyl to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:

50.

11. Herbicidal composition, according to claim 1, characterized in that at least one compound selected from the group of compounds X is flufenoxymacyl.

12. Herbicidal composition, according to claim 11, characterized in that the weight ratio of flufenoxymacyl to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:

50.

13. Herbicidal composition, according to claim 1, characterized in that at least one compound selected from the group of compounds X is a compound represented by the formula - Petition 870250084601, dated 09 / 19 / 2025, page 85 / 98 3 / 5 Ia (D- 14. Herbicidal composition, according to claim 13, characterized in that the weight ratio between the compound represented by formula (I) and an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:

50.

15. Herbicidal composition according to claim 1, characterized in that at least one compound selected from the group of compounds X is a compound represented by formula (II).

16. Herbicidal composition, according to claim 15, characterized in that the weight ratio between the compound represented by formula (II) and an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:

100.

17. Method for weed control, characterized in that it comprises a step of simultaneous or separate application, in any order, of at least one compound selected from the following group of compounds X and a salt of 2,4-D trolamine: Group of compounds X: group consisting of flumioxazin, trifludimoxazin, saflufenacil, thiafenacil, flufenoximacil, a compound represented by the following formula (I): ° (D and a compound represented by the following formula (II): (II). Petition 870250084601, dated 19 / 09 / 2025, page 86 / 98 4 / 5 18. Method according to claim 17, characterized in that the weight ratio of at least one compound selected from the group of compounds X to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:1 to 1:

100.

19. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is flumioxazin.

20. Method according to claim 19, characterized in that the weight ratio of flumioxazin to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:5 to 1:

20.

21. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is trifludimoxazine.

22. Method according to claim 21, characterized in that the weight ratio of trifludimoxazine to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:

100.

23. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is saflufenacil.

24. Method according to claim 23, characterized in that the weight ratio of saflufenacil to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:

50.

25. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is thiaphenacyl.

26. Method according to claim 25, characterized in that the weight ratio of thiaphenacyl to an equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:

50.

27. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is flufenoxymacyl.

28. Method according to claim 27, characterized in that the weight ratio of flufenoxymacyl to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:

50.

29. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is a compound represented by formula (I).

30. Method according to claim 29, characterized in that the weight ratio of the compound represented by formula (I) to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:10 to 1:

50.

31. Method according to claim 17, characterized in that at least one compound selected from the group of compounds X is a compound represented by formula (II).

32. Method according to claim 31, characterized in that the weight ratio of the compound represented by formula (II) to an acid equivalent of a 2,4-D trolamine salt is within a range of 1:15 to 1:

100. Petition 870250084601, dated 09 / 19 / 2025, pp. 88 / 98