METHOD FOR TREATING OR INHIBITING PLANT INFECTION AND COMPOSITION FOR TREATING A PLANT DISEASE

Description

1 / 47 METHOD FOR TREATING OR INHIBITING PLANT INFECTION AND COMPOSITION FOR TREATING A PLANT DISEASE Separated from BR112020003023-6, filed on August 15, 2018. FIELD OF TECHNIQUE

[001] The present description refers to compositions and treatments for plant diseases. BACKGROUND

[002] Huanglongbing (HLB), also known as citrus greening, yellow bud, or yellow dragon, is a bacterial disease that affects citrus plants. In Florida, HLB was a major contributor to the decline in orange production between 2003 and 2016, as traditional plant pest and disease management systems have not been adequate in treating HLB. SUMMARY

[003] The present invention is based, in part, on the development of novel methods for the treatment of HLB, among other plant diseases. The treatments involve administering to plants, for example, citrus plants, compositions rich in polyphenols (such as proanthocyanidins (PACs)), benzoic acid, quinic acid and / or xyloglucans, such as cranberry compositions. The compositions can be administered to plants, for example, citrus plants, in various ways, for example, injected into an affected plant, sprayed onto an affected plant, implanted into an affected plant, injected into the soil around an affected plant and / or sprayed into the soil around an affected plant. The compositions can be combined with a carrier for improved delivery of the compositions into the plant phloem, where HLB bacteria reside. The compositions can offer the advantage of being easily incorporated into an integrated pest management system already in use in agriculture. Petition 870260043312, dated 08 / 05 / 2026, p. 10 / 129 2 / 47 citrus fruits. The compositions may offer the advantage of being of natural origin and therefore can be used in the production of organic plant products.

[004] Therefore, in one aspect, the present specification provides a composition for the treatment of a plant disease including phenolics of 1 mg / g and 100 mg / g and an adjuvant that allows the composition to be delivered to at least part of the phloem of a citrus plant.

[005] In some implementations, the composition includes quinic acid from 0.01% to 14%. In some cases, the composition includes quinic acid from about 0.1% to about 4%.

[006] In some implementations, the composition includes at least one of malic acid and citric acid in 0.01% to 20%. In some cases, the composition includes citric acid in about 0.1% to about 4%. In some cases, the composition includes malic acid in about 0.1% to about 3%.

[007] In some implementations, the composition includes proanthocyanidins from 1,000 mg / kg to 100,000 mg / kg. In some cases, the composition includes proanthocyanidins from about 2,000 mg / kg to about 5,000 mg / kg. In some cases, the composition includes proanthocyanidins from about 30,000 mg / kg to about 60,000 mg / kg.

[008] In some implementations, the composition includes at least one of a countercurrent extraction concentrate, a co-current extraction concentrate, a concentrated wort, an ultrafiltration retentate, a resin extract, and a subcritical water extraction from leaves, press cake, or fruit.

[009] In some implementations, the composition is derived from cranberries.

[0010] In some implementations, the composition is derived from one or more of the following: cranberries, blueberries, elderberry, chokeberries, linden Petition 870260043312, dated 08 / 05 / 2026, page 11 / 129 3 / 47 gonberries, raspberries, grapes, currants, huckleberries, strawberries, blackberries, cloudberries, blackcurrants, whitecurrants, and cinnamon bark, or any combination thereof.

[0011] In some implementations, the adjuvant includes a surfactant.

[0012] In some implementations, the composition includes a starch-degrading enzyme, in which the adjuvant is configured to deliver the starch-degrading enzyme to at least a portion of the plant phloem. In some cases, the starch-degrading enzyme is an amylase.

[0013] In some implementations, the adjuvant includes at least one of a mineral oil, a horticultural oil, a vegetable oil, and a seed oil.

[0014] In some implementations, the adjuvant includes a tannase enzyme.

[0015] In some implementations, the composition includes at least one antibiotic.

[0016] In some implementations, the composition includes at least one of β-aminobutyric acid, 2-deoxy-D-glucose, salicylic acid, oxalic acid, trehalose, trehalose-6-phosphate, or any combination thereof.

[0017] In some implementations, the composition includes at least one insecticide.

[0018] In some implementations, the composition includes a cranberry solids content of about 1 Brix.

[0019] In some implementations, the composition includes a cranberry solids content of approximately 10 Brix.

[0020] In some implementations, the composition includes a cranberry solids content of approximately 20 Brix.

[0021] In some implementations, the composition includes antocia Petition 870260043312, dated 08 / 05 / 2026, page 12 / 129 4 / 47 ranges from about 1 mg / kg to about 100 mg / kg.

[0022] In some implementations, the composition includes anthocyanins from about 2,000 mg / kg to about 5,000 mg / kg.

[0023] In some implementations, the composition includes total phenolics of about 3 mg / g to about 12 mg / g.

[0024] In some implementations, the composition includes total phenolics of about 35 to about 65 mg / g.

[0025] In some implementations, the composition includes a buffer.

[0026] In another aspect, the present specification provides a method for treating or inhibiting the infection of a plant by a plant pathogen, including contacting the plant with an amount of a composition effective in inhibiting the plant pathogen, wherein the composition includes phenolics from 1 mg / g to 100 mg / g.

[0027] In some implementations, contacting the plant includes brushing or spraying the composition onto the plant, or injecting the composition into the plant.

[0028] In some implementations, contacting the plant includes spraying, soaking, mixing, or injecting the composition into the soil around the plant.

[0029] In some implementations, the composition is derived from cranberries.

[0030] In some implementations, the composition is derived from one or more of the following: cranberries, blueberries, elderberry, chokeberries, lingonberries, raspberries, grapes, currants, huckleberries, strawberries, blackberries, cloudberries, blackcurrants, whitecurrants, and cinnamon bark, or any combination thereof.

[0031] In some implementations, the composition includes a carrier that allows the composition to be delivered to the plant's phloem.

[0032] In some implementations, the carrier includes by the Petition 870260043312, dated 08 / 05 / 2026, p. 13 / 129 5 / 47 contains a mineral oil, a horticultural oil, a vegetable oil, and a seed oil.

[0033] In some implementations, the composition includes a starch-degrading enzyme. In some cases, the starch-degrading enzyme is an amylase.

[0034] In some implementations, the composition includes a tannase enzyme.

[0035] In some implementations, the composition includes at least one antibiotic.

[0036] In some implementations, the plant is a citrus plant and the plant pathogen is Candidatus Liberibacter.

[0037] In some implementations, the composition includes a cranberry solids content of about 1 Brix.

[0038] In some implementations, the composition includes a cranberry solids content of about 10 Brix.

[0039] In some implementations, the composition includes a cranberry solids content of approximately 20 Brix.

[0040] In some implementations, the composition includes at least one of citric and malic acid from 0.01% to 20%. In some cases, the composition includes citric acid from about 0.1% to about 4%. In some cases, the composition includes malic acid from about 0.1% to about 3%.

[0041] In some implementations, the composition includes quinic acid from about 0.01% to about 14%. In some cases, the composition includes quinic acid from about 0.1% to about 4%.

[0042] In some implementations, the composition includes anthocyanins from about 1 mg / kg to about 100 mg / kg.

[0043] In some implementations, the composition includes anthocyanins from about 2,000 mg / kg to about 5,000 mg / kg.

[0044] In some implementations, the composition includes compre Petition 870260043312, dated 08 / 05 / 2026, p. 14 / 129 6 / 47 contains proanthocyanidins from about 1,000 mg / kg to about 100,000 mg / kg. In some cases, the composition includes proanthocyanidins from about 2,000 mg / kg to about 5,000 mg / kg. In some cases, the composition includes proanthocyanidins from about 30,000 mg / kg to about 60,000 mg / kg.

[0045] In some cases, the composition includes total phenolics of about 3 mg / g to about 12 mg / g. In some cases, the composition comprises total phenolics of about 35 to about 65 mg / g.

[0046] In another aspect, the present specification provides a composition for the treatment of a plant disease including phenolics from 1 mg / g to 100 mg / g, wherein the composition is in the form of a fertilizer spike, a solid fertilizer mixture, a liquid fertilizer mixture, a soil spray, a soil comprising the composition, a sprayer, a gel, an injectable composition, or an implantable composition.

[0047] In some implementations, the composition includes at least one of malic acid and citric acid from 0.01% to 20%.

[0048] In some implementations, the composition includes quinic acid from 0.01% to 14%.

[0049] In some implementations, the composition includes proanthocyanidins from 1,000 mg / kg to 100,000 mg / kg.

[0050] In some implementations, the composition is derived from cranberries.

[0051] In another aspect, the present specification provides a cranberry composition for the treatment of a plant disease that includes phenolics from 1 mg / g to 100 mg / g, quinic acid from 0.5% to 2% and proanthocyanidins in more than 20,000 mg / kg.

[0052] In another aspect, the present specification provides a cranberry composition for the treatment of a plant disease. Petition 870260043312, dated 08 / 05 / 2026, p. 15 / 129 7 / 47 which includes phenolics from 1 mg / ga to 100 mg / ge and proanthocyanidins in more than 20,000 mg / kg.

[0053] In another aspect, the present specification provides a cranberry composition for the treatment of a plant disease that includes phenolics from 1 mg / g to 100 mg / g, optionally includes quinic acid from 0.5% to 2% and optionally includes proanthocyanidins in more than 20,000 mg / kg.

[0054] In another aspect, the present specification provides a cranberry composition for the treatment of a plant disease which includes phenolics from 1 mg / g to 100 mg / g, total organic acids (citric acid, malic acid and quinic acid) from 0.5% to 6% and proanthocyanidins in more than 20,000 mg / kg.

[0055] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one skilled in the art to which this invention pertains. Methods and materials are described herein for use in the present invention; other suitable methods and materials known in the art may also be used. The materials, methods, and examples are illustrative only and are not intended to be limiting. All publications, patent applications, patents, sequences, database entries, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, this specification, including definitions, shall prevail.

[0056] Other features and advantages of the invention will be evident from the description, the detailed figures that follow, and the claims. BRIEF DESCRIPTION OF THE FIGURES

[0057] Figure 1 is a schematic of a system for treating plants affected with HLB using cranberry compositions.

[0058] Figure 2 is a flowchart for a treatment method. Petition 870260043312, dated 08 / 05 / 2026, page 16 / 129 8 / 47 of plants affected with HLB with cranberry compositions.

[0059] Figure 3 is a bar graph showing the percentage of inhibition of L. crescens in vitro with various cranberry compositions at concentrations of 1, 2 and 4% solids.

[0060] Figure 4 is a bar graph showing the minimum inhibitory concentration of three cranberry compositions in vitro against L. crescens.

[0061] Figure 5 is a bar graph showing the number of CLa copies present over time in leaves treated with cranberry compositions, as determined by qPCR.

[0062] Figure 6 is a bar graph showing a change in PMA titer over time in leaves treated with cranberry compositions.

[0063] Figures 7A and 7B are photographs of leaves sprayed with cranberry compositions immediately after application and after 48 hours, respectively.

[0064] Figures 8A and 8B are photographs of citrus leaves soaked in control buffer solution at the time of initial treatment and after seven days, respectively.

[0065] Figures 9A and 9B are photographs of citrus leaves soaked in SWE of cranberry filter cake extract at the time of initial treatment and after seven days, respectively. DETAILED DESCRIPTION

[0066] This description describes, for example, methods for treating and preventing the spread of plant pathogens, such as the bacterial pathogen that causes HLB. Traditional methods of treating plant diseases or pests, including insecticides and antibiotics, generally do not work well in treating HLB as the HLB bacteria α-proteobacteria Candidates Liberibacter spp. (also known as CLas or Las) reside in the phloem of Petition 870260043312, dated 08 / 05 / 2026, p. 17 / 129 9 / 47 infected plant. Pairing a composition described herein, such as a cranberry composition, with a suitable phloem carrier provides improved treatment for HLB. The composition and carrier can be delivered to plants via a variety of methods, such as sprays, liquids, aerosols, solid compositions, and / or injectables, among others. In some cases, a composition described herein may not require a carrier to provide treatment for HLB.

[0067] Generally, the methods involve administering an effective amount of a composition described herein to a plant in need of treatment, or which has been determined to be in need of such treatment. As used in this context, treatment means improving at least one symptom of a plant disease, such as HLB, and / or reducing the number, viability and / or growth rate of a plant pathogen, for example, CLas, on the plant. For example, in some cases of HLB treatment, the treatment may result in reduced yellowing in one or more parts of the plant, for example, in the leaves, compared to untreated plants infected with CLas. In other cases, the treatment may result in increased fruit production (e.g., measurable in terms of the number and / or size of the fruit) compared to untreated plants infected with CLas.In other cases, treatment with a composition described here may result in a reduced rate of symptom appearance compared to untreated plants. In still other cases for HLB treatment, the treatment may result in a reduction in the lower Brix and higher acidity, typically associated with citrus greening. In still other cases, the treatment may result in increased Ct values ​​(a measure of CLas titration) when using qPCR to analyze a sample obtained from an infected plant. Petition 870260043312, dated 08 / 05 / 2026, page 18 / 129 10 / 47

[0068] An effective amount is an amount sufficient to effect desired benefits or results. For example, a therapeutic amount is that which achieves the desired therapeutic effect. This amount may be the same as or different from a prophylactically effective amount needed to prevent the onset of disease or disease symptoms. An effective amount may be administered in one or more administrations, applications, or dosages. Compositions may be administered from one or more times a day to one or more times a week; including once every two days, once a month, or once a year. Those skilled in the art will appreciate that certain factors may influence the amount and time required for the effective treatment of a plant, including, but not limited to, the severity of the disease, previous treatments, general health, age and / or type of plant, and the presence of other plant diseases.

[0069] In one example, in cranberry compositions, the concentration of cranberry solids in the composition may be approximately between 1 and 65 Brix, for example, about 1 Brix to about 2 Brix, about 2 Brix to about 4 Brix, about 4 Brix to about 10 Brix, about 10 Brix to about 20 Brix, about 20 to about 30 Brix, about 30 to about 40 Brix, about 40 to about 50 Brix, about 50 to about 60 Brix, about 60 to about 65 Brix, about 60 to about 65 Brix, or about 1 Brix, 2 Brix, 4 Brix, 10 Brix, 20 Brix, 30 Brix, 40 Brix, 50 Brix, 60 Brix or 65 Brix.

[0070] Depending on the treatment method used, varying amounts of cranberry composition can be applied to the plant. For example, in a foliar application (application of liquid to the leaves of a plant) between approximately 20 and 500 gallons of cranberry composition can be applied per acre, where an acre can include approximately 80 to 130 trees for older groves and approximately 140 to 220 trees for new groves. The amount can Petition 870260043312, dated 08 / 05 / 2026, page 19 / 129 11 / 47 may be less when fewer than 80 trees are present per acre. In another example, in a trunk injection, between about 10 ml and about 3 L may be injected per 254 cm (100 inches) diameter trunk. In another example, when using a soil spray application method, about 1 to about 500 gallons may be applied per acre, where an acre includes approximately 80 to 130 trees. The amount may be less when fewer than 80 trees are present per acre. Treatments may be performed as often as necessary by those skilled in the art, for example, between 1 and about 16 times per year, for example, 1, 2, 3, 4, etc. times per year.

[0071] Plants treatable with the compositions described herein, for example, cranberry compositions, include, but are not limited to, citrus plants, for example, oranges, tangerines, grapefruits, lemons and their hybrids. Plant pathogens that can be treated with the compositions described herein, for example, cranberry compositions, include bacteria, viruses, fungi and nematodes, among others. These plant pathogens can attack any plant tissue, including leaves and roots. Description of the disease

[0072] HLB generally causes yellowing of veins and tissues of infected plants, leaf spots, defoliation, root rot, and ultimately plant death. Affected plants may also have stunted growth and may produce small, irregularly shaped fruits with partially green peels. HLB is a bacterial disease spread by psyllid insects (also known as jumping lice). The HLB bacterium, a tedious, phloem-limited prokaryotic αproteobacterium, Candidatus Liberibacter spp. (CLas or often called Las), primarily Ca. L. asiaticus, Ca. L. africanus, and Ca. L. americanus. Ca. L. Petition 870260043312, dated 08 / 05 / 2026, p. 20 / 129 12 / 47 asiaticus, and its vector Diaphorina citri, also known as Asian Citrus Psyllid (ACP), are the primary cause of HLB disease in North America. Psyllids ingest HLB bacteria while consuming plant tissue from an HLB-infected plant and transmit the HLB bacteria to uninfected plants in the process of ingesting tissue from the uninfected plant. In an infected plant, the HLB bacteria reside in the plant's phloem. Phloem is the vascular tissue of plants that conducts sugars and other metabolic products down from the leaves. The HLB bacteria metabolize the sugar that would otherwise be transported through the phloem to other parts of the plant, leading to stunted growth and yellowing of plant parts. Furthermore, CLas create starchy materials in the phloem, further reducing the ability of antibacterial agents to reach the CLas themselves.

[0073] Those skilled in the art will appreciate that other plant diseases caused by phloem-restricted bacteria, such as Liberibacter in addition to CLas, namely Candidates Liberibacter solanaceous, known to cause diseases in potatoes and carrots, and Phlomobacter, such as Candidates Phlomobacter fragariae, identified as causing Marginal Chlorosis of strawberries, could also be treated with the compositions and methods described herein. In addition to the proteobacteria above, cell wall-less bacteria, also known as mollicutes, such as phytoplasmas and spiroplasmas, can also be treated with the compositions and methods described herein. Exemplary plant diseases caused by phytoplasmas include, for example, lethal yellowing diseases of palm and peach, such as Peach Rosette, Little Peach, Yellow Peach, and Red Sutures.Exemplary plant diseases caused by spiroplasmas include, for example, Citrus Stubborn Disease (caused by Spiroplasma citri) and Corn Stunting Disease (caused by Spiroplasma kunkelii). Petition 870260043312, dated 08 / 05 / 2026, p. 21 / 129 13 / 47

[0074] Those skilled in the art will appreciate that other plant diseases, such as those residing in the xylem, generally progress to the phloem and can also be treated with the compositions and methods described herein. For example, Pierce's Disease (caused by Xyllella fastidiosa) and Tomato Canker (caused by Cornebacterium michiganense) can be treated with cranberry compositions. Because delivery to a plant's xylem rather than its phloem is generally easier, the concentrations of cranberry solids in the composition applied to the plant can be lower than in the treatment of a phloem-based disease.

[0075] In addition, fungal diseases such as Soybean Rust (caused by Phakopsora pachyrhizi), Laurel Wilt of woody plants (Raffaelea lauricola) and Sudden Oak Death (caused by Phytophthora ramorum) and viruses such as Corn Streak (caused by tenuivirus) can be treated with cranberry compositions. Cranberry Compounds for Disease Inhibition

[0076] Compositions rich in polyphenols, such as proanthocyanidins, or benzoic acid or quinic acid or xyloglucans or a combination thereof, are effective against plant pathogens such as CLas. Cranberry compositions are particularly useful in the methods currently described. A cranberry composition is a composition comprising a component of, or a component derived from, a cranberry plant, such as a cranberry, which exhibits at least one antibacterial property (e.g., a bactericidal or bacteriostatic property). The activity of the cranberry composition against CLas may include, for example, action as an antimicrobial agent, a biofilm breaker and / or quorum sensing mechanism, or as an anti-adhesion agent to disrupt the microbiome.

[0077] Various cranberry compositions may have different levels Petition 870260043312, dated 08 / 05 / 2026, p. 22 / 129 14 / 47 tes of acids (e.g., citric, malic, and quinic acids) and phenolics, including anthocyanins and PACs. Phenolics include polyphenols and phenolic acids. Within the polyphenols, there are flavonoids and other polyphenols, such as resveratrol. Flavonoids can be flavans or polyflavanols, such as proanthocyanidins (PACs). Flavans can be anthocyanins, or flavonols. Cranberry compositions suitable for treating plant diseases, such as HLB, can be produced by known cranberry processing methods. For example, cranberry compositions may include those derived from a subcritical water extraction (SWE) of cranberry filter cake, leaves, and / or fruit. In another example, cranberry compositions may include a concentrate from a countercurrent extraction (CCE) process. Several examples of cranberry compositions useful in the methods currently described are listed below in Table 1. Table 1. Properties of Cranberry Extract. Type of Extract Brix Citric % Malic % Quinic % Anthocyanins (mg / kg) PACs (mg / kg) Total Phenolics (mg / g) Conc. CCE 22.11 3.23 2.39 3.27 76.15 2151.4 4.48 Must Conc. 20.99 2.84 2.10 3.02 49.55 4742.9 7.4 Resin Extract 18.49 0.27 0.15 0.20 4248.64 59831.7 61.96 Resin and Tanase Extract 18.28 0.27 0.15 0.20 3968.54 50584.96 76.38 Retentate UF 22.05 2.61 1.44 2.07 2419.4 40443.6 14.99 Filter Cake SWE 20.00 0.13 0.08 0.08 10.69 45056.8 40.96 Leaf SWE 17.37 0.24 0.25 0.87 1.9 30871.2 5.02 Fruit SWE (total acid) 19.24 2.71 1.76 2.74 4.05 2269.4 8.05 Fruit SWE (partial acid) 18.74 2.41 1.55 2.59 10.56 4335.9 10.72

[0078] CCE concentrate is a concentrate created by a propitiation 870260043312, dated 08 / 05 / 2026, page 23 / 129 15 / 47 Countercurrent extraction process. CCE concentrate can be created by extracting cranberry juice from thawed frozen fruit that has first been sliced ​​or scarified, using running water in a countercurrent extractor. An extract produced during countercurrent extraction can then be concentrated by ultrafiltration and evaporation. Details of an exemplary countercurrent extraction process can be found in U.S. Patent No. 5,419,251. An exemplary CCE concentrate may have a solids content of about 20 to 25 Brix, about 2 to 4% citric acid, about 2 to 3% malic acid, about 3 to 4% quinic acid, and may contain about 70 to 80 mg / kg of anthocyanins, about 2,000 to 2,500 mg / kg of PACs, and about 4 to 5 mg / g of total phenolics. An exemplary CCE concentrate derived from cranberries is shown in Table 1 above.

[0079] Concentrated must can be created by crushing cranberry fruit using a hammer mill, digesting the pectin using pectinase, and decanting or pressing the digested material. An extract can then be concentrated by ultrafiltration and evaporation. An exemplary concentrated must may have a solids content of about 20 to 25 Brix, about 2 to 3% citric acid, about 2 to 3% malic acid, about 2 to 4% quinic acid, and may contain about 40 to 60 mg / kg of anthocyanins, about 4,000 to 5,000 mg / kg of PACs, and about 7 to 8 mg / g of total phenolics. An exemplary concentrated must derived from cranberries is shown in Table 1 above. Resin extract can be created through an anion-exchange chromatography process to remove acids from a cranberry juice stream.One method for producing resin extract may involve the adsorption of organic acids (e.g., citric, malic, and quinic acids) onto a single anion exchange resin or series of resins and elution of each acid type from the mixture. Petition 870260043312, dated 08 / 05 / 2026, page 24 / 129 16 / 47 aqueous separately. In one example, citric acid and malic acid are linked by a first resin and quinic acid is linked by a second resin. In some implementations, an anion exchange may occur outside a column, for example, in a batch adsorption process. An exemplary resin extract may have a solids content of about 18 to 20 Brix, about 0 to 1% citric acid, about 0 to 1% malic acid, about 0 to 1% quinic acid, and may contain about 4,000 to 5,000 mg / kg of anthocyanins, about 50,000 to 70,000 mg / kg of PACs, and about 50 to 70 mg / g of total phenolics. An exemplary resin extract derived from cranberries is shown in Table 1 above.

[0080] The resin extract can be mixed with a tannase enzyme. The tannase enzyme assists in the hydrolysis of PACs present in the resin extract to lower molecular weights. Approximately 0.5% of the tannase enzyme (Sumizyme TAN) is mixed with resin extract to form a solution. An exemplary resin extract and tannase mixture may have a solids content of about 16 to 20 Brix, about 0 to 1% citric acid, about 0 to 1% malic acid, about 0 to 1% quinic acid, and may contain about 4,000 to 5,000 mg / kg anthocyanins, about 40,000 to 60,000 mg / kg PACs, and about 70 to 80 mg / g total phenolics. An exemplary resin extract derived from cranberries is shown in Table 1 above.

[0081] Ultrafiltration (UF) retentate is a solution that includes suspended solids and high molecular weight solutes, including proanthocyanidins, and can be created by ultrafiltration of CCE concentrate. In some examples, UF retentate can be produced by filtering CCE concentrate through an ultrafiltration membrane system with a molecular weight limit between 2,000 and 10,000 Daltons. An exemplary UF retentate may have a solids content of about 20 to 25 Brix, about 2 to 3% citric acid, about 1 to Petition 870260043312, dated 08 / 05 / 2026, p. 25 / 129 17 / 47 2% malic acid, about 1 to 3% quinic acid, and may contain about 2,000 to 3,000 mg / kg of anthocyanins, about 30,000 to 50,000 mg / kg of PACs, and about 12 to 16 mg / g of total phenolics. An exemplary UF retentate derived from cranberries is shown in Table 1 above.

[0082] Subcritical water extractions (SWE) of filter cake, leaves, and fruits can also be used. SWE extractions can be created by treating the filter cake, leaves, or fruits at about 160°C and about 2000 psi pressure. An exemplary SWE concentrate made from filter cake may have a solids content of about 18 to 22 Brix, about 0 to 1% citric acid, about 0 to 1% malic acid, about 0 to 1% quinic acid, and may contain about 5 to 15 mg / kg of anthocyanins, about 40,000 to 50,000 mg / kg of PACs, and about 35 to 45 mg / g of total phenolics. An exemplary SWE concentrate made from leaves may have a solids content of about 16 to 20 Brix, about 0 to 1% citric acid, about 0 to 1% malic acid, about 0 to 1% quinic acid, and may contain about 1 to 3 mg / kg of anthocyanins, about 25,000 to 35,000 mg / kg of PACs, and about 4 to 6 mg / g of total phenolics.An exemplary SWE concentrate made from fruits with a high acid content may have a solids content of about 18 to 22 Brix, about 2 to 4% citric acid, about 1 to 3% malic acid, about 2 to 4% quinic acid, and may contain about 3 to 5 mg / kg of anthocyanins, about 1,000 to 3,000 mg / kg of PACs, and about 7 to 9 mg / g of total phenolics. An exemplary SWE concentrate made from low-acid fruits may have a solids content of about 16 to 20 Brix, about 2 to 3% citric acid, about 1 to 3% malic acid, about 2 to 4% quinic acid, and may contain about 5 to 15 mg / kg of anthocyanins, about 4,000 to 5,000 mg / kg of PACs, and about 8 to 12 mg / g of total phenolics. Exemplary SWE extractions. Petition 870260043312, dated 08 / 05 / 2026, p. 26 / 129 18 / 47 derivatives of cranberries are shown in Table 1 above. Details of an exemplary process for making SWE extractions can be found in US Patent Application Publication No. 2018 / 0220680.

[0083] A co-current concentrate is a concentrate created by a co-current extraction process. A co-current concentrate can be created by extracting fruit juice in a co-current extractor, for example, a DTS system (designed by Dutch TecSource). Those skilled in the art will appreciate that a co-current concentrate can include similar proportions of phenolics, organic acids, and PACs as the exemplary CCE concentrate discussed above.

[0084] Those skilled in the art will appreciate that the methods described above and others may be modified and / or combined as necessary to produce a composition described herein, and the resulting compositions may be adjusted, for example, diluted, concentrated, selectively enriched, etc., as appropriate for particular treatments.

[0085] In some examples, a composition for the treatment of plant diseases, as described herein, includes a Brix content of about 1 to 65 Brix, for example, about 1 Brix to about 2 Brix, about 1 Brix to about 5 Brix, about 1 Brix to about 10 Brix, about 1 Brix to about 20 Brix, about 1 Brix to about 30 Brix, about 1 Brix to about 40 Brix, about 1 Brix to about 50 Brix, about 1 Brix to about 50 Brix, about 1 Brix to about 60 Brix, about 1 Brix to about 65 Brix, about 2 Brix to about 5 Brix, about 2 Brix to about 10 Brix, about 2 Brix to about 20 Brix, about 2 Brix to about 30 Brix, about 2 Brix to about 40 Brix, about 2 Brix to about 50 Brix, about 2 Brix to about 50 Brix, about 2 Brix to about 60 Brix, about 2 Brix to about 65 Brix, about Petition 870260043312, dated 08 / 05 / 2026, page 27 / 129 19 / 47 from 5 Brix to about 10 Brix, about 5 Brix to about 20 Brix, about 5 Brix to about 30 Brix, about 5 Brix to about 40 Brix, about 5 Brix to about 50 Brix, about 5 Brix to about 60 Brix, about 5 Brix to about 65 Brix, about 10 Brix to about 20 Brix, about 10 Brix to about 30 Brix, about 10 Brix to about 40 Brix, about 10 Brix to about 50 Brix, about 10 Brix to about 60 Brix, about 10 Brix to about 65 Brix, about 20 to about 30 Brix, about 20 Brix to about 40 Brix, about 20 Brix to about 50 Brix, about 20 Brix to about 60 Brix, about 20 Brix to about Brix, approximately 30 to approximately 40 Brix, approximately 30 Brix to approximately 50 Brix, about 30 Brix to about 60 Brix, about 30 Brix to about Brix, from about 40 Brix to about 50 Brix, from about 40 to about 60 Brix, from about 40 Brix to about 65 Brix, from about 50 to about 60 Brix, approximately 50 to approximately 65 Brix, approximately 60 to approximately 65 Brix, or approximately 1 Brix, 2 Brix, 4 Brix, 10 Brix, 20 Brix, 30 Brix, 40 Brix, 50 Brix, 60 Brix, or 65 Brix. In some examples, a composition for the treatment of plant diseases as described herein may be a dry composition.

[0086] In some examples, a composition for the treatment of plant diseases as described herein includes anthocyanins from 0 to 35,000 mg / kg, for example, about 0 mg / kg to about 5 mg / kg, about 0 mg / kg to about 25 mg / kg, about 0 mg / kg to about 50 mg / kg, about 0 mg / kg to about 100 mg / kg, about 0 mg / kg to about 500 mg / kg, about 0 mg / kg to about 1,000 mg / kg, about 0 mg / kg to about 2,500 mg / kg, about 0 mg / kg to about 5,000 mg / kg, about 0 to about 10,000 mg / kg, about 0 mg / kg to about 35,000 mg / kg, about 5 mg / kg to about 25 mg / kg, about 5 mg / kg to about 50 mg / kg, about 5 mg / kg to about 100 mg / kg, about 5 mg / kg to about 500 mg / kg, about 5 mg / kg to about 1,000 mg / kg, about 5 mg / kg to about 2,500 mg / kg, about Petition 870260043312, dated 08 / 05 / 2026, page 28 / 129 20 / 47 from 5 mg / kg to about 5,000 mg / kg, about 5 to about 10,000 mg / kg, about 5 mg / kg to about 35,000 mg / kg, about 25 mg / kg to about 50 mg / kg, about 25 mg / kg to about 100 mg / kg, about 25 mg / kg to about 500 mg / kg, about 25 mg / kg to about 1,000 mg / kg, about 25 mg / kg to about 2,500 mg / kg, about 25 mg / kg to about 5,000 mg / kg, about 25 to about 10,000 mg / kg, about 25 mg / kg to about 35,000 mg / kg, about 50 mg / kg to about 100 mg / kg, about 50 mg / kg to about 500 mg / kg, about 50 mg / kg to about 1,000 mg / kg, about 50 mg / kg to about 2,500 mg / kg, about 50 mg / kg to about 5,000 mg / kg, about 50 to about 10,000 mg / kg, about 50 mg / kg to about 35,000 mg / kg, about 100 mg / kg to about 500 mg / kg, about 100 mg / kg to about 1,000 mg / kg, about 100 mg / kg to about 2,500 mg / kg, about 100 mg / kg to about 5,000 mg / kg, about 100 to about 10,000 mg / kg, about 100 mg / kg to about 35.000 mg / kg, about 500 mg / kg to about 1,000 mg / kg, about 500 mg / kg to about 2,500 mg / kg, about 500 mg / kg to about 5,000 mg / kg, about 500 to about 10,000 mg / kg, about 500 mg / kg to about 35,000 mg / kg, about 1,000 mg / kg to about 2,500 mg / kg, about 1,000 mg / kg to about 5,000 mg / kg, about 1,000 to about 10,000 mg / kg, about 1,000 mg / kg to about 35,000 mg / kg, about 2,500 mg / kg to about 5,000 mg / kg, about 2,500 to about from 10,000 mg / kg, approximately 2,500 mg / kg to approximately 35,000 mg / kg, approximately 5,000 to approximately 10,000 mg / kg, approximately 5,000 mg / kg to approximately 35,000 mg / kg, approximately 10,000 mg / kg to approximately 35,000 mg / kg, or approximately 0 mg / kg, approximately 5 mg / kg, approximately 25 mg / kg, approximately 50 mg / kg, approximately 100 mg / kg, approximately 500 mg / kg, approximately 1,000 mg / kg, approximately 2,500 mg / kg, approximately 5,000 mg / kg, approximately 10,000 mg / kg or approximately 35,000 mg / kg.

[0087] In some examples, a composition for treatment Petition 870260043312, dated 08 / 05 / 2026, p. 29 / 129 21 / 47 of plant diseases as described herein includes proanthocyanidins (PACs) from 0 to 500,000 mg / kg, for example, approximately 0 mg / kg to approximately 2,500 mg / kg, approximately 0 mg / kg to approximately 5,000 mg / kg, approximately 0 mg / kg to approximately 10,000 mg / kg, approximately 0 mg / kg to approximately 25,000 mg / kg, approximately 0 mg / kg to approximately 50,000 mg / kg, approximately 0 mg / kg to approximately 75,000 mg / kg, approximately 0 mg / kg to approximately 100,000 mg / kg, approximately 0 mg / kg to approximately 200,000 mg / kg, approximately 0 mg / kg to approximately 300,000 mg / kg, approximately 0 mg / kg to approximately 400,000 mg / kg, about 0 mg / kg to about 500,000 mg / kg, about 2,500 mg / kg to about 5,000 mg / kg, about 2,500 mg / kg to about 10,000 mg / kg, about 2,500 mg / kg to about 25,000 mg / kg, about 2,500 mg / kg to about 50,000 mg / kg, about 2,500 mg / kg to about 75,000 mg / kg, about 2,500 mg / kg to about 100,000 mg / kg, about 2,500 mg / kg to about 200,000 mg / kg, about 2,500 mg / kg to about 300,000 mg / kg, about 2,500 mg / kg to approximately 400.000 mg / kg, about 2,500 mg / kg to about 500,000 mg / kg, about 5,000 mg / kg to about 10,000 mg / kg, about 5,000 mg / kg to about 25,000 mg / kg, about 5,000 mg / kg to about 50,000 mg / kg, about 5,000 mg / kg to about 75,000 mg / kg, about 5,000 mg / kg to about 100,000 mg / kg, about 5,000 mg / kg to about 200,000 mg / kg, about 5,000 mg / kg to about 300,000 mg / kg, about 5,000 mg / kg to about 400,000 mg / kg, about 5,000 mg / kg to approximately 500,000 mg / kg, approximately 10,000 mg / kg to approximately 25,000 mg / kg, approximately 10,000 mg / kg to approximately 50,000 mg / kg, approximately 10,000 mg / kg to approximately 75,000 mg / kg, approximately 10,000 mg / kg to approximately 100,000 mg / kg, approximately 10,000 mg / kg to approximately 200,000 mg / kg, approximately 10,000 mg / kg to approximately 300,000 mg / kg, approximately 10,000 mg / kg to approximately 400,000 mg / kg, approximately 10,000 mg / kg to approximately 500,000 mg / kg, approximately 25,000 mg / kg to approximately 50,000 mg / kg, about 25,000 mg / kg to about 75,000 mg / kg, about 25.000 mg / kg a cerPetição 870260043312, dated 08 / 05 / 2026, pág. 30 / 129. 22 / 47 ca. 500,000 mg / kg, about 50,000 mg / kg to about 75,000 mg / kg, about 50,000 mg / kg to about 100,000 mg / kg, about 50,000 mg / kg to about 200,000 mg / kg, about 50,000 mg / kg to about 300,000 mg / kg, about 50,000 mg / kg to about 400,000 mg / kg, about 50,000 mg / kg to about 500,000 mg / kg, about 75,000 mg / kg to approximately 100,000 mg / kg, approximately 75,000 mg / kg to approximately 200,000 mg / kg, approximately 75,000 mg / kg to approximately 300,000 mg / kg, approximately 75,000 mg / kg to approximately 400,000 mg / kg, approximately 75,000 mg / kg to approximately 500,000 mg / kg, approximately 100,000 mg / kg to approximately 200,000 mg / kg, approximately 100,000 mg / kg to approximately 300,000 mg / kg, approximately 100,000 mg / kg to approximately 400,000 mg / kg, approximately 100,000 mg / kg to approximately 500,000 mg / kg, approximately from 200,000 mg / kg to about 300,000 mg / kg, about 200,000 mg / kg to about 400,000 mg / kg, about 200,000 mg / kg to about 500,000 mg / kg, about 300,000 mg / kg to about 400,000 mg / kg, about 300,000 mg / kg to about 500,000 mg / kg, about 400,000 mg / kg to about 500,000 mg / kg, or about 0 mg / kg, about 2,500 mg / kg, about 5,000 mg / kg, about 10,000 mg / kg, about 25,000 mg / kg, about 50,000 mg / kg, about 75,000 mg / kg, about 100,000 mg / kg, about 200,000 mg / kg, about 300,000 mg / kg, about 400,000 mg / kg or about 500,000 mg / kg.The proanthocyanidin concentrations in Table 1 were measured using the Ocean Spray dimethylaminocinnamaldehyde (DMAC) method, as described in US Patent Application Publication 2017 / 0181458A1.

[0088] In some examples, a composition useful for the treatment of plant diseases, as described herein, includes total phenolics from 0 to 700 mg / g, for example, about 0 mg / g, about 5 mg / g, about Petition 870260043312, dated 08 / 05 / 2026, p. 31 / 129 23 / 47 of 0 mg / g to approximately 10 mg / g, approximately 0 mg / g to approximately 25 mg / g, approximately 0 mg / g to approximately 50 mg / g, approximately 0 mg / g to approximately 75 mg / g, approximately 0 mg / g to approximately 100 mg / g, approximately 0 mg / g to approximately 150 mg / g, approximately 0 mg / g to approximately 200 mg / g, approximately 0 mg / g to approximately 400 mg / g, approximately 0 mg / g to approximately 600 mg / g, approximately 0 mg / g to approximately 700 mg / g, approximately 5 mg / g to approximately 10 mg / g, approximately 5 mg / g to approximately 25 mg / g, approximately 5 mg / g to approximately 50 mg / g, approximately 5 mg / g to approximately 75 mg / g, approximately 5 mg / g to approximately 100 mg / g, about 5 mg / g, about 150 mg / g, about 5 mg / g, about 200 mg / g, about 5 mg / g, about 400 mg / g, about 5 mg / g, about 600 mg / g, about 5 mg / g, about 700 mg / g, about 10 mg / g, about 25 mg / g, about 10 mg / g, about 50 mg / g, about 10 mg / g, about 75 mg / g, about 10 mg / g, about 100 mg / g, about 10 mg / g, about 150 mg / g, about 10 mg / g, about 200 mg / g, about 10 mg / g, about 400 mg / gapproximately 10 mg / g, approximately 600 mg / g, approximately 10 mg / g, approximately 700 mg / g, approximately 25 mg / g, approximately 50 mg / g, approximately 25 mg / g, approximately 75 mg / g, approximately 25 mg / g, approximately 100 mg / g, approximately 25 mg / g, approximately 150 mg / g, approximately 25 mg / g, approximately 200 mg / g, approximately 25 mg / g, approximately 400 mg / g, approximately 25 mg / g, approximately 600 mg / g, approximately 25 mg / g, approximately 700 mg / g, approximately 50 mg / g, approximately 75 mg / g, approximately 50 mg / g, approximately 100 mg / g, approximately 50 mg / g, approximately 150 mg / g, approximately 50 mg / g, approximately 200 mg / g, about 50 mg / g about 400 mg / g, about 50 mg / g about 600 mg / g, about 50 mg / g about 700 mg / g, about 75 mg / g about 100 mg / g, about 75 mg / g about 150 mg / g, about 75 mg / g about 200 mg / g, about 75 mg / g about 400 mg / g, about 75 mg / g about 600 mg / g, about 75 mg / g about 700 mg / g, about 100 mg / g about 150 mg / g, about 100 mg / g about 200 mg / g, about 100 mg / g about 400 mg / g,approximately 100 mg / g, Petition 870260043312, dated 08 / 05 / 2026, page 32 / 129 24 / 47 to about 600 mg / g, about 100 mg / g to about 700 mg / g, about 150 mg / g to about 200 mg / g, about 150 mg / g to about 400 mg / g, about 150 mg / g to about 600 mg / g, about 150 mg / g to about 700 mg / g, about 200 mg / g to about 400 mg / g, about 200 mg / g to about 600 mg / g, about 200 mg / g to about 700 mg / g, about 400 mg / g to about 600 mg / g, about 400 mg / g to about 700 mg / g, about 600 mg / g to about 700 mg / g, or about 0 mg / g, about 5 mg / g, about 10 mg / g, approximately 25 mg / g, approximately 50 mg / g, approximately 75 mg / g, approximately 100 mg / g, approximately 150 mg / g, approximately 200 mg / g, approximately 400 mg / g, approximately 600 mg / g, or approximately 700 mg / g. The total phenolics for the compositions described here were measured by the Folin-Ciocalteu method.

[0089] In some examples, a composition for the treatment of plant diseases, as described herein, includes citric acid from 0% to 20%, for example, about 0% to about 0.5%, about 0% to about 1%, about 0% to about 1.5%, about 0% to about 2%, about 0% to about 2.5%, about 0% to about 3%, about 0% to about 4%, about 0% to about 5%, about 0% to about 10%, about 0% to about 15%, about 0% to about 20%, about 0.1% to about 0.5%, about 0.1% to about 1%, about 0.1% to about 1.5%, about 0.1% to about 2%, about 0.1% to about 2.5%, about 0.1% to about 3%, about 0.1% to about 4%, about 0.1% to about 5%, about 0.1% to about 5%, about 0.1% to about 10%, about 0.1% to about 15%, about 0.1% to about 20%, about 0.5% to about 1%, about 0.5% to about 1.5%, about 0.5% to about 2%, about 0.5% to about 2.5%, about 0.5% to about 3%, about 0.5% to about 4%,about 0.5% to about 5%, about 0.5% to about 5%, about 0.5% to about 10%, about 0.5% to about 15%, about 0.5% to about 20%, about 1% to about 1.5%, about, Petition 870260043312, dated 08 / 05 / 2026, page 33 / 129 25 / 47 from 1% to about 2%, about 1% to about 2.5%, about 1% to about 3%, about 1% to about 4%, about 1% to about 5%, about 1% to about 5%, about 1% to about 10%, about 1% to about 15%, about 1% to about 20%, about 1.5% to about 2%, about 1.5% to about 2.5%, about 1.5% to about 3%, about 1.5% to about 4%, about 1.5% to about 5%, about 1.5% to about 5%, about 1.5% to about 10%, about 1.5% to about 15%, about 1.5% to about 20%, approximately 2% to approximately 2.5%, approximately 2% to approximately 3%, approximately 2% to approximately 4%, approximately 2% to approximately 5%, approximately 2% to approximately 10%, approximately 2% to approximately 15%, approximately 2% to approximately 20%, approximately 2.5% to approximately 3%, approximately 2.5% to approximately 4%, approximately 2.5% to approximately 5%, approximately 2.5% to approximately 10%, approximately 2.5% to approximately 15%, approximately 2.5% to approximately 20%, approximately 3% to approximately 4%, approximately 3% to approximately 5%, approximately 3% to approximately 5%, approximately 3% to approximately 10%,about 3% to about 15%, about 3% to about 20%, about 4% to about 5%, about 4% to about 10%, about 4% to about 15%, about 4% to about 20%, about 5% to about 10%, about 5% to about 15%, about 5% to about 20%, about 10% to about 15%, about 10% to about 20%, about 15% to about 20%, or about 0.1%, about 0.5%, about 1%, about 1.5%, about 2%, about 2.5%, about 3%, about 4%, about 5%, about 10%, about 15% or about 20%.

[0090] In some examples, a composition for the treatment of plant diseases, as described herein, includes malic acid from 0% to 20%, for example, about 0% to about 0.5%, about 0% to about 1%, about 0% to about 1.5%, about 0% to about 2%, about 0% to about 2.5%, about 0% to about 3%, about 0% to about 4%, about 0% to about 5%, about 0% to Petition 870260043312, dated 08 / 05 / 2026, page 34 / 129 26 / 47 close to 10%, close to 0% to close to 15%, close to 0% to close to 20%, close to 0.1% to close to 0.5%, close to 0.1% to close to 1%, close to 0.1% to close to 1.5%, close to 0.1% to close to 2%, close to 0.1% to close to 2.5%, close to 0.1% to close to 3%, close to 0.1% to close to 4%, close to 0.1% to close to 5%, close to 0.1% to close to 5%, close to 0.1% to close to 10%, close to 0.1% to close to 15%, close to 0.1% to close to 20%, close to 0.5% to close to 1%, close to 0.5% to close to 1.5%, approximately 0.5% to approximately 2%, approximately 0.5% to approximately 2.5%, approximately 0.5% to approximately 3%, approximately 0.5% to approximately 4%, approximately 0.5% to approximately 5%, approximately 0.5% to approximately 5%, approximately 0.5% to approximately 10%, approximately 0.5% to approximately 15%, approximately 0.5% to approximately 20%, approximately 1% to approximately 1.5%, approximately 1% to approximately 2%, approximately 1% to approximately 2.5%, approximately 1% to approximately 3%, approximately 1% to approximately 4%, approximately 1% to approximately 5%, approximately 1% to approximately 10%, approximately 1% to approximately 15%,from approximately 1% to approximately 20%, from approximately 1.5% to approximately 2%, from approximately 1.5% to approximately 2.5%, from approximately 1.5% to approximately 3%, from approximately 1.5% to approximately 4%, from approximately 1.5% to approximately 5%, from approximately 1.5% to approximately 10%, from approximately 1.5% to approximately 15%, from approximately 1.5% to approximately 20%, from approximately 2% to approximately 2.5%, from approximately 2% to approximately 3%, from approximately 2% to approximately 4%, from approximately 2% to approximately 5%, from approximately 2% to approximately 10%, from approximately 2% to approximately 15%, from approximately 2% to approximately 20%, from approximately 2.5% to approximately 3%, from approximately 2.5% to approximately 4%, about 2.5% to about 5%, about 2.5% to about 5%, about 2.5% to about 10%, about 2.5% to about 15%, about 2.5% to about 20%, about 3% to about 4%, about 3% to about 5%, about 3% to about 5%, about 3% to about 10%, about 3% to about 15%, about 3% to about 20%, about 4% to about 5%, about 4% to about 10%, about 4% to about 15%, about 4% to about 20%, about 5% to about 10%, about 5% to, Petition 870260043312, dated 08 / 05 / 2026, page 35 / 129 27 / 47 about 15%, about 5% to about 20%, about 10% to about 15%, about 10% to about 20%, about 15% to about 20%, or about 0.1%, about 0.5%, about 1%, about 1.5%, about 2%, about 2.5%, about 3%, about 4%, about 5%, about 10%, about 15%, or about 20%.

[0091] In some examples, a composition for the treatment of plant diseases, as described herein, includes quinic acid from 0% to 14%, for example, about 0% to about 0.5%, about 0% to about 1%, about 0% to about 1.5%, about 0% to about 2%, about 0% to about 2.5%, about 0% to about 3%, about 0% to about 4%, about 0% to about 5%, about 0% to about 10%, about 0% to about 14%, about 0.1% to about 0.5%, about 0.1% to about 1%, about 0.1% to about 1.5%, about 0.1% to about 2%, about 0.1% to about 2.5%, about 0.1% to about 3%, about 0.1% to about 4%, about 0.1% to about 5%, about 0.1% to about 5%, about 0.1% to about 10%, about 0.1% to about 14%, about 0.5% to about 1%, about 0.5% to about 1.5%, about from 0.5% to about 2%, about 0.5% to about 2.5%, about 0.5% to about 3%, about 0.5% to about 4%, about 0.5% to about 5%, about 0.5% to about 10%,from approximately 0.5% to approximately 14%, from approximately 1% to approximately 1.5%, from approximately 1% to approximately 2%, from approximately 1% to approximately 2.5%, from approximately 1% to approximately 3%, from approximately 1% to approximately 4%, from approximately 1% to approximately 5%, from approximately 1% to approximately 10%, from approximately 1% to approximately 14%, from approximately 1.5% to approximately 2%, from approximately 1.5% to approximately 2.5%, from approximately 1.5% to approximately 3%, from approximately 1.5% to approximately 4%, from approximately 1.5% to approximately 5%, from approximately 1.5% to approximately 5%, from approximately 1.5% to approximately 10%, from approximately 1.5% to approximately 14%, from approximately 2% to approximately 2.5%, from about 2% to about 3%, from about 2% to about 4%, from about 2% to about 5%, from about 2% to about 10%, from about 2% to, Petition 870260043312, dated 05 / 08 / 2026, page. 36 / 129 28 / 47 about 14%, about 2.5% to about 3%, about 2.5% to about 4%, about 2.5% to about 5%, about 2.5% to about 5%, about 2.5% to about 10%, about 2.5% to about 14%, about 3% to about 4%, about 3% to about 5%, about 3% to about 5%, about 3% to about 10%, about 3% to about 14%, about 4% to about 5%, about 4% to about 10%, about 4% to about 15%, about 4% to about 20%, about 5% to about 10%, about 5% to about 14%, about 10% to about of 14%, or about 0.1%, about 0.5%, about 1%, about 1.5%, about 2%, about 2.5%, about 3%, about 4%, about 5%, about 10% or about 14%.

[0092] In some examples, a composition for the treatment of plant diseases, as described herein, includes benzoic acid from 0 to 30,000 ppm, for example, about 0 ppm to about 1,000 ppm, about 0 ppm to about 2,000 ppm, about 0 ppm to about 5,000 ppm, about 0 ppm to about 10,000 ppm, about 0 ppm to about 15,000 ppm, about 0 ppm to about 20,000 ppm, about 0 ppm to about 25,000 ppm, about 0 to about 30,000 ppm, about 1,000 ppm to about 5,000 ppm, about 1,000 ppm to about 10,000 ppm, about 1,000 ppm to about 15,000 ppm, about 1,000 ppm to about 20,000 ppm, about 1,000 ppm to about 25,000 ppm, about 1,000 to about 30,000 ppm, about 2,000 ppm to about 5,000 ppm, about 2,000 ppm to about 10,000 ppm, about 2,000 ppm to about 15,000 ppm, about 2,000 ppm to about 20,000 ppm, about 2,000 ppm to about 25,000 ppm, about 2,000 to about 30,000 ppm, about 5,000 ppm to about 10,000 ppm, about 5.000 ppm to about 15,000 ppm, about 5,000 ppm to about 20,000 ppm, about 5,000 ppm to about 25,000 ppm, about 5,000 to about 30,000 ppm, about 10,000 ppm to about 15,000 ppm, about 10,000 ppm to about 20,000. Petition 870260043312, dated 08 / 05 / 2026, page 37 / 129 29 / 47 ppm, about 10,000 ppm to about 25,000 ppm, about 10,000 to about 30,000 ppm, about 15,000 ppm to about 20,000 ppm, about 15,000 ppm to about 25,000 ppm, about 15,000 to about 30,000 ppm, about 20,000 ppm to about 25,000 ppm, about 20,000 to about 30,000 ppm, about 25,000 ppm to about 30,000 ppm, or about 0 ppm, about 1,000 ppm, about 2,000 ppm, about 5,000 ppm, about 10,000 ppm, approximately 15,000 ppm, approximately 20,000 ppm, approximately 25,000 ppm, or approximately 30,000 ppm.

[0093] Based on the desired application method for delivering the compositions to the plant, as will be described below, the compositions described above may be diluted or concentrated accordingly.Those skilled in the art will appreciate that the compositions may also include one or more additives, for example, antibiotics such as amoxicillin, amphotericin, ampicillin, ceftriaxone, chloramphenicol, ciprofloxacin, clotrimazole, gentamicin, ketoconazole, mupirocin, oxytetracycline, sulfadimethoxine, streptomycin, vancomycin, or any combination thereof, surfactants such as non-ionic surfactants, for example, alcohol alkoxylates, alkyl amine ethoxylates, alkyl phenol ethoxylates, fatty acids or oil-based or sorbitan ethoxylates, polyalkene oxide copolymers or any combination thereof. Alternatively or in addition, anionic surfactants may be used, such as alkylbenzene sulfonates, alkyl naphthalene sulfonates, alkyl sulfonates, fatty acid sulfonates, ligno sulfonates and dioctyl sulfosuccinates or any combination thereof. Additives may include starch-degrading enzymes, adjuvants, oils, etc.Those skilled in the art will appreciate that the inclusion of additives may affect the range of properties of the composition described above.

[0094] Those skilled in the art will appreciate that the composition may be in concentrated, diluted, or dry form. For example, the compounds Petition 870260043312, dated 08 / 05 / 2026, page 38 / 129 30 / 47 solutions as described herein can be concentrated into a powder that retains the same ratio of components (e.g., PACs, total phenolics, acids, etc.) as described. Depending on the composition form, more or less of the composition may be required for application to an affected plant. Methods for Measuring Phenolics in Compositions

[0095] Total phenolics for the compositions described herein were measured by the Folin-Ciocalteu method. In an exemplary method, Folin-Ciocalteu Phenol Reagent 2N (MP Biomedical LLC) was diluted to a 10x solution in deionized water and stored in an amber bottle. Anhydrous sodium carbonate (Sigma-Aldrich) was dissolved in deionized water to a 7.5% solution and then placed on a heated stirring plate until well mixed. The mixture was allowed to cool to room temperature and then stored in an amber bottle. Samples were diluted in deionized water. 100 µL of the prepared samples were added to glass culture tubes. 100 µL of deionized water were added to a glass culture tube to be used as a blank assay. 3.9 mL of deionized water were then added to each tube and vortexed.250 µL of 10x 2N Folin-Ciocalteu reagent were then added to each glass culture tube and vortexed. 750 µL of 7.5% sodium carbonate solution were added to each glass culture tube and then vortexed. The samples were then stored in a dark cabinet for 30 minutes. The samples were removed and then measured at 765 nm on a Hach DR3900 (1.13 cm long) using the deionized water sample as a blank assay (Singleton, V. and Rossi, J. 1965. Colorimetry of Total Phenolics with Phosphomolybdic Phosphotungstic Acid Reagents. Am J. Eno e Vitic. 16: 144 to 158).

[0096] A 0.5% gallic acid solution was prepared (Sigma Petition 870260043312, dated 08 / 05 / 2026, page 39 / 129 31 / 47 Aldrich). A standard curve containing 0 to 200 mg / L was made and measured as indicated above. Any sample result that was outside the absorbance range of the standard curves was rerun with a better dilution to obtain an absorbance within the standard curve range. A control sample that had been tested and verified was also measured at each run. Results are presented as mg / g of gallic acid equivalent (GAE). Methods for Measuring Organic Acids in Compositions

[0097] The organic acid content analyses of the compositions described herein were performed on a Dionex ICS-2100 ion chromatography system (Thermo Scientific Sunnyvale, CA, USA) equipped with an EG40 eluent generator, an ASRS 300, a 4 mm suppressor, and a CR-ATC. The separation of organic acids was performed on an IonPac AS11-HC analytical ion exchange RFIC column, 4 x 250 mm, connected from an IonPac AG11-HC 4 x 50 mm RFIC shielding column and measured by suppressed conductivity detection. A stock standard containing citric acid, fumaric acid, galactronic acid, isocitric acid, malic acid, and quinic acid is prepared in deionized water at a concentration of 5%. The stock standard is then diluted to prepare working standards of 5, 25, and 50 mg / L, which are used to calibrate the instrument. Methods for Measuring Proanthocyanidins in Compositions

[0098] The PAC levels in the compositions described herein were evaluated or quantified using the Ocean Spray dimethyl cinnamaldehyde (DMAC) method, which uses fractionated PACs from specific cranberry sources as a standard. See, for example, Martin et al., Food Res Int 71: 68–82, 2015; and Pascual-Teresa et al., J. Agric Food Chem 46: 4209–4213, 1998, which are incorporated herein by reference. Those skilled in the art will appreciate that other methods may be used. For example, Brunswick Labs uses a standard of pro Petition 870260043312, dated 08 / 05 / 2026, page 40 / 129 32 / 47 Pure cyanidin A-2 / B-2 (dimer) commercially available as a standard. See, for example, Prior et al., J Sci Food Agric 90: 1473–1478, 2010. When a sample is tested for PACs using the Ocean Spray method compared to the Brunswick Labs method, lower PAC values ​​are consistently found using the Brunswick Labs method due to the lower molecular weight of the procyanidin A-2 / B-2 standard compared to the fractionated PAC standard, which contains A-2 / B-2 and all the higher molecular weight PAC oligomers and polymers present in cranberries. For example, a sample that has approximately 110 mg of PACs when tested using the Ocean Spray DMAC method may have only about 36 mg of PACs when tested using the Brunswick Labs DMAC method.

[0099] Those skilled in the art will recognize that both methods measure the total PACs in a sample, but differ only in the way the results are expressed based on the chosen standard equivalence. Therefore, although results may differ when using the two methods on the same sample, this does not mean that the levels are different. As a result, it is recommended that test comparisons of the same material always be made using the same methodology to avoid confusion. Both methods are commonly used in industry. However, the Ocean Spray method better represents the PACs found in cranberry, and therefore, the results obtained by this method are a truer representation of the 'real' amount of PACs in cranberry samples. Consequently, all PAC values ​​described herein are determined using the DMAC Ocean Spray method.

[00100] In an example of performing the DMAC method for Ocean Spray, a Sephadex LH-20 column separation followed by absorbance measurement of the DMAC colorimetric reaction was used to Petition 870260043312, dated 08 / 05 / 2026, page 41 / 129 33 / 47 To determine the PAC content in cranberry samples (Cunningham, D., Vannozzi, S., O'Shea, E., & Turk, R. 2002. Quality Management of Nutraceuticals ACS Symposium series 803, Washington DC.). A 1.0 g aqueous sample was loaded onto a Sephadex LH-20 polyprep prehydration column. Distilled water (10 mL), followed by 25% EtOH solution (10 mL) were used to elute sugars, organic acids, anthocyanins, and flavonols. These eluents were discarded. The columns were then sequentially washed with 70% acetone in aqueous solution (2.5 mL and then another 2.5 mL), collected, and combined in a 15 mL tube. The samples were vortexed, and then 1 mL of the sample was added to a glass culture tube. DMAC at 1% (in 70 / 30 MeOH / HCl) was prepared by adding 3 mL to each culture tube, including the reagent blank.The tubes were vortexed and then the absorbance was measured after 5 min at 640 nm on a Hach DR3900. A reagent blank assay was used to zero the spectrophotometer. Samples needed to be in the ideal range of 0.2 to 0.8, with samples outside this range being run again with dilutions consequently adjusted. PAC concentrations of the samples were calculated as is and reported in mg / kg. Sources for Disease-Inhibiting Compositions

[00101] In addition to compositions derived from cranberry, those skilled in the art will appreciate that compositions useful in the methods currently described can be made from other fruits or plant products with similar properties. For example, as an alternative to, or in addition to, cranberry plant components, the composition may include compositions derived from blueberries, elderberry, chokeberries, lingonberries, raspberries, grapes, currants, huckleberries, strawberries, blackberries, cloudberries, blackcurrants, whitecurrants, and / or any mixture thereof. In some examples, the composition may include Petition 870260043312, dated 08 / 05 / 2026, page 42 / 129 34 / 47 compositions derived from cinnamon bark. These compositions are referred to here as blueberry, elderberry, chokeberry, lingonberry, raspberry, grape, currant, huckleberry, strawberry, blackberry, cloudberry, blackcurrant, whitecurrant, and cinnamon bark compositions, respectively.

[00102] The exemplary compositions in Table 1 were derived from cranberries; however, those skilled in the art will appreciate that compositions with similar Brix values, citric acid, malic acid, and quinic acid content, and / or phenolic content (anthocyanins and PACs) can be produced from other fruits or vegetable products, such as those listed above. Furthermore, those skilled in the art will appreciate that, in some cases, processes similar to those used in the manufacture of the compositions described in Table 1 can be used on the fruit or vegetable products listed below. For example, countercurrent extraction can be performed on blueberries or other fruits to produce a CCE concentrate similar to the CCE concentrate produced from cranberries, shown in Table 1. Delivery to the Plant Phloem and Compositions for Such Delivery

[00103] Some plant diseases, such as HLB, are particularly difficult to treat because the plant pathogen, such as CLas, resides in the phloem. Phloem is the vascular tissue in plants that conducts sugars and other metabolic products down from the leaves. In order to deliver cranberry compositions so that they can come into contact with and thus inhibit phloem-resident pathogens, the compositions may be mixed with additional ingredients, i.e., carriers, for better transport to the phloem. Carriers may include adjuvants. Adjuvants may be activating adjuvants (enhancing the activity of the cranberry component) or modifying adjuvants (enhancing the shelf life, handling, and application of the cranberry components). Petition 870260043312, dated 08 / 05 / 2026, p. 43 / 129 35 / 47

[00104] In one example of the use of an activating adjuvant, compositions, such as cranberry compositions, can be mixed with surfactants for improved spreading and flowability. Surfactants generally decrease the surface tension between two liquids (or between a liquid and a solid) and can allow for better delivery of the compositions to the leaf cuticle and / or phloem. Useful surfactants include, for example, nonionic surfactants such as alcohol alkoxylates, alkyl amine ethoxylates, alkyl phenol ethoxylates, fatty acids or oil-based, polyalkene oxide copolymers, or any combination thereof. Alternatively or in addition, anionic surfactants may be used, such as alkylbenzene sulfonates, alkyl naphthalene sulfonates, alkyl sulfonates, fatty acid sulfonates, linguosulinates and dioctyl sulfosuccinates, or any combination thereof.

[00105] In an example of the use of a modifying adjuvant, compositions, such as cranberry compositions, may have additional benzoic acid, propionic acid, sorbic acid and / or its salts, parabens, such as methyl, ethyl, butyl and propyl paraben, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) or a combination thereof to improve the shelf life of the composition.

[00106] In another example of the use of a modifying adjuvant, compositions, such as cranberry compositions, may include water that has been treated with, for example, polyglucosides, polyglycosides, polyoxyethylene glycol or its derivatives, ammonium salts, citrophosphate, glycerol acids, hydroxycarboxylic acids, phosphate esters, phosphoric acid, polycarboxylic acid, polyacrylic acid, sulfates, urea or any combination thereof.

[00107] In another example, oils, such as mineral oil (petroleum-based), horticultural oil or vegetable oils (seed oils) and their esterified derivatives, or mixtures of oils, may be used. Petition 870260043312, dated 08 / 05 / 2026, page 44 / 129 36 / 47 as adjuvants. An emulsifier can be used to mix hydrophilic ingredients with oil. The emulsion formed can be an emulsion, a microemulsion, or a nanoemulsion. Oils can facilitate transport to the phloem because they can mask the polarity of the compositions, such as cranberry compositions.

[00108] In another example, ammonium salts, such as ammonium sulfate, can be used as an adjuvant. Ammonium salts are believed to improve the absorption of pesticides.

[00109] In another example, starch-degrading enzymes, such as α-amylase, can be added to the cranberry composition. Since the HLB bacterium, CLas, produces starchy materials within the phloem, the introduction of a starch-degrading enzyme can improve the distribution of cranberry compositions throughout the phloem. Furthermore, the introduction of a starch-degrading enzyme can provide additional space for the cranberry compositions to come into contact with CLas, improving bacterial inhibition.

[00110] In another example, a tannase enzyme can be added to the cranberry composition. A tannase enzyme could be, for example, Sumizyme TAN. The tannase enzyme helps in the hydrolysis of PACs present in the cranberry compositions into smaller molecular weights. A greater number of lower molecular weight PAC molecules can not only improve the effectiveness of the inhibition, but is also more likely to reach the phloem where disease-causing pathogens, such as bacteria and vectors, reside.

[00111] In another example, the composition may include one or more buffers, such as citrate and / or malate, to slightly increase the pH of the composition. Sodium and potassium hydroxides may be used to create citrate and malate buffers due to citric and malic acid already being present in the composition. In other cases, a commercially available buffer, such as phosphate and Imidazole-HCl, may be added. Petition 870260043312, dated 08 / 05 / 2026, page 45 / 129 37 / 47 related to the composition.

[00112] In another example, the composition may include β-aminobutyric acid (BABA), 2-deoxy-D-glucose (DDG), salicylic acid (SA), oxalic acid (OA), trehalose or trehalose-6-phosphate, or any mixture and / or salts thereof. Such compounds may act as hormones to revive tree tissues damaged by HLB.

[00113] In some instances, adjuvants included in the composition may be naturally derived. Naturally derived adjuvants include, but are not limited to, oils including horticultural and vegetable oils, and surfactants including cassava extract, Castile soap, saponaria, and quillaja bark extract. Compositions, for example, cranberry compositions, which include only ingredients of natural origin, may be used in organic farming to produce organic plant products. Methods of Administering Compounds to Plants

[00114] The compositions can be applied to plants in various ways, as illustrated in Figure 2. For example, cranberry compositions can be applied to citrus plants by spraying. Mechanical or hydraulic sprayers consisting of a tank, a pump, a lance (for single nozzles), and a nozzle (or multiple nozzles) can be used. For example, cranberry ingredients in liquid form can be spread on the foliage as pressurized droplets, which can be large raindrops or tiny, almost invisible particles. A schematic of a spraying system for treating an affected plant with cranberry compositions is illustrated in Figure 1. Additionally or alternatively, cranberry compositions can be applied to affected plants, or to the surrounding soil using aerosols, liquids, sprayers, or injections. Cranberry compositions can be applied by brushing the compositions onto leaves or other parts of the plants or Petition 870260043312, dated 08 / 05 / 2026, page 46 / 129 38 / 47 applied manually to the plants.

[00115] The compositions described herein can be introduced via stem implantation or stem injection. Injecting or implanting the composition directly into the stem can aid in delivering the composition to the plant's phloem. Due to the proximity of the composition to the phloem when the composition is injected or implanted into the stem, disease inhibition results can be produced more rapidly than by other application methods. Furthermore, compositions with higher concentrations of larger molecules (e.g., PACs) may benefit from being injected or implanted into the stem, as these larger molecules do not need to diffuse far enough to reach the bacteria in the phloem.

[00116] The compositions can be introduced into plants via soil injection, soil spraying, doping, and / or basal soil spraying. The compositions can be absorbed into the plant through the roots and delivered to the phloem.

[00117] Compositions may be included in solid compositions, such as spikes, pellets, powders, dusts, etc. Compositions may be mixed with fertilizers or soils. Compositions mixed in fertilizers or soil mixtures may be solid (e.g., pellets, powders) or liquid (e.g., solutions, pastes, gels). In some implementations, a solid composition may be configured to dissolve upon contact with water, for example, as a time-release formulation. When the solid composition is brought into contact with water, the composition may be released into the soil surrounding a plant and absorbed by the plant. Solid compositions, for example, fertilizers, pellets, spikes, may be configured to release a predetermined dose of compositions over a defined period of time. Those skilled in the art will appreciate that the dosing of these solid compositions may Petition 870260043312, dated 08 / 05 / 2026, page 47 / 129 39 / 47 can be defined based on the level of infection, plant type, soil environment, etc.

[00118] Depending on the treatment method used, varying amounts of the composition may be applied to the plant. Furthermore, the amount or frequency of application of the composition may be affected by the age of the plant or the severity of the disease being treated. Treatment in Psyllids

[00119] The compositions described herein can be used to inhibit CLas bacteria that grow in the gut of psyllids, specifically Asian citrus psyllids (ACPs). Psyllids transfer CLas bacteria between plants as they move and feed on the plants. As a psyllid ingests plant tissue that has been treated with a cranberry composition, the antibacterial properties of the cranberry composition can inhibit the CLas bacteria growing in the psyllid's gut. A psyllid lacking CLas bacteria in its gut does not transfer the bacteria from plant to plant. As such, as psyllids ingest the tissue of treated plants, and the bacteria carried by the psyllids are inhibited, disease spread can be limited.

[00120] While traditional insecticides aim to repel psyllids from plants to prevent the spread of disease, in some cases it may be beneficial to attract psyllids to treated plants so that the bacteria carried by the psyllids can be inhibited. Compositions that are attractive to psyllids, for example, citric acid, can be included in the cranberry composition. Volatile compositions that attract psyllids can also be used. For example, psyllids are also attracted to the Indian curry leaf, Bergera koenigii. Volatile compositions from these plants can also be added to cranberry ingredients to increase psyllid attraction. Petition 870260043312, dated 08 / 05 / 2026, page 48 / 129 40 / 47

[00121] Those skilled in the art will appreciate that the compositions described in this document can be used for other phloem-bound vectors, such as leafhoppers, and xylem-bound vectors, such as sharpshooters. Plant pathogens can also be inhibited in vectors such as aphids, leafhoppers, mealybugs, whiteflies, and membranous insects.

[00122] A trap that includes a composition as described here can also be used to attract the vector and inhibit the vector's intestinal pathogens. Integrated Pest Management Systems

[00123] The methods currently described can be used as part of an integrated pest management (IPM) program. Integrated pest management programs, including treatments with cranberry compositions, for example, include treating plants and vectors with cranberry ingredients along with biological control, mechanical, cultural, physical / environmental modification, and host resistance or genetic manipulation. For example, cranberry compositions can be administered together with antibiotics. Antibiotics that can be used with the compositions described here include oxytetracycline, streptomycin, amoxicillin, amphotericin, ampicillin, ceftriaxone, chloramphenicol, ciprofloxacin, clotrimazole, gentamicin, ketoconazole, mupirocin, sulfadimycintoxin, vancomycin. This combination can allow for a reduction in the total amount of antibiotics used in the treatment of citrus plants.For example, the introduction of cranberry compositions can reduce the amount of agricultural streptomycin (Firewall®) and agricultural oxytetracycline (Fireline®) applied to plants due to a synergistic effect between the antibiotics and the cranberry compositions. EXAMPLES Example 1: In Vitro Inhibitory Activity of Cranberry Compositions Petition 870260043312, dated 08 / 05 / 2026, page 49 / 129 41 / 47 berry

[00124] Liberibacter crescens is the closest relative in culture of Candidates Liberibacter asiaticus (CLas), the suggested causative agent for citrus greening, or Huanglongbing (HLB). An exemplary assay was performed to bring Liberibacter crescens into contact with the various cranberry extracts described herein at different concentrations. The aim of this assay was to identify agents that could inhibit the growth of the pathogen and prevent the spread of HLB. Since CLas cannot grow outside its hosts, L. crescens was used as a surrogate to test potential treatments.

[00125] In an exemplary assay procedure, L. crescens was cultured in 2 mL of BM7 medium in small test tubes to the exponential phase (3 to 4 days). The BM7 medium includes α-ketoglutarate (2 g), ACES buffer (10 g), KOH (3.75 g), and distilled water (550 mL) (after sterilization and cooling of this medium, 150 mL of fetal bovine serum and 300 mL of TMN-FH medium (Sigma T1032) are added to form the BM7 medium). 500 µL of cells cultured in the exponential phase were added to 20 mL of fresh BM7 and aliquoted into 96-well plates. The L. crescens cultures were then treated with the test composition (here, a cranberry extract) at the different concentrations being tested in triplicate, and the cultures were incubated.

[00126] After incubation, an appropriate sample of the culture was transferred and Presto Blue™ Cell Viability Reagent was added to each sample. Presto Blue™ Cell Viability Reagent uses a non-fluorescent blue resazurin-based compound that is reduced to resorfuin, a red fluorescent compound, in the cytosol of a viable cell. The fluorescence measurement reflects the conversion of the compound and is proportional to the amount of metabolite cells. Petition 870260043312, dated 08 / 05 / 2026, page 50 / 129 42 / 47 clinically active. After adding Presto Blue™ Cell Viability Reagent to each sample, the samples were incubated and the fluorescence of each sample was read using an HT Synergy Biotek microplate reader.

[00127] After collecting the fluorescence data, the replicate wells (those treated with the same compound at the same concentration) were averaged, and the readings were normalized by subtracting the average fluorescence from the wells treated only with media. The fluorescence data were then compared to no-treatment wells to find a relative percentage inhibition for each plate. As such, the exact readings are not comparable between runs, but the relative percentages are comparable. The inhibition results for 20%, 10%, and 5% dilutions of various cranberry extracts are shown below in Table 2. Table 2. Inhibition of Liberibacter crescens using cranberry extracts. Extract Type Dilution Inhibition Dilution Inhibition Dilution Inhibition Cone. CCE 20% 15.44 10% 8.15 5% 1.54 Wort Cone. 20% 79.23 10% 36.36 5% 12.38 Resin extract 20% 100.79 10% 100.06 5% 94.83 Retentate UF 20% 99.26 10% 96.92 5% 87.06 Filter cake SWE 20% 100.04 10% 98.21 5% 95.36 Leaf SWE 20% 99.65 10% 97.51 5% 92.02 Fruit SWE (total acid) 20% 96.40 10% 89.62 5% 70.17 Fruit SWE (partial acid) 20% 97.41 10% 95.49 5% 83.64

[00128] As shown in Table 2, a variety of cranberry extracts, upon contact with Liberibacter crescens, produced Petition 870260043312, dated 08 / 05 / 2026, page 51 / 129 43 / 47 inhibition of 80% or more. Cranberry extract dilutions that produced between 80% and 90% inhibition are shaded light gray, and cranberry extract dilutions that produced more than 90% inhibition are shaded dark gray. Without intending to be limited by theory, it is believed that PACs and phenolics have a greater impact on the inhibition of Liberibacter crescens than the acids in cranberry extracts, for example, malic, citric, and quinic acids. However, it is also believed that weak acids present in cranberry extracts may be beneficial in delivering the cranberry extract to the phloem due to the lower dissociation constants of weak acids. The dilutions shown in Table 2 correspond to dilutions of compositions similar to the exemplary compositions shown in Table 1, which are approximately 20 Brix.Therefore, a 20% dilution corresponds to approximately 4 Brix, a 10% dilution corresponds to approximately 2 Brix, and a 5% dilution corresponds to approximately 1 Brix. Example 2: In Vitro Inhibitory Activity of Cranberry Compositions

[00129] Cranberry compositions with solids contents of 0.2%, 0.5%, 1%, 2%, and 4% were tested to determine the inhibitory properties of each cranberry composition, as shown in Figures 3 and 4. These cranberry compositions were produced using the processes described in Example 1. The minimum inhibitory concentration was 1 Brix (1% solids content) for each of the cranberry compositions. Oxytetracycline was used as a control and provided 100% inhibition at each level tested. Example 3: In Vivo Inhibitory Activity of Cranberry Compositions

[00130] Three compositions of cranberry, Resin Extract, Resin Extract and Tanase, and Filter Cake Extract SWE were added as a soil spray to ten young citrus plants with average trunk diameters of 0.7 cm. Each cranberry composition Petition 870260043312, dated 08 / 05 / 2026, page 52 / 129 44 / 47 had a solids content of 10 Brix. Water and streptomycin were used as negative and positive controls, respectively. Leaf samples were collected (three per plant) immediately before application of the cranberry compositions. qPCR was conducted on pre-treatment leaf samples, as well as on leaf samples collected two days, fourteen days, and twenty-eight days after treatment.

[00131] The qPCR process was completed on an Applied Biosystems 7500 rapid real-time PCT system. Each reaction was conducted with 1 pL of DNA sample in a total reaction volume of 26 pL. The cox2 gene was measured as an internal plant control. After initial application of the cranberry compositions, plants were tested for CLas by qRT-PCR and for visual toxicity.

[00132] Figure 5 shows the copy number of CLas genes present in leaf samples over time, as determined by qPCR analysis of the leaf samples. All changes in copy number and CT titer were reported as changes from baseline. Initially, plants treated with cranberry and streptomycin compositions had a high copy number compared to plants treated with water. However, after 28 days, plants treated with streptomycin along with plants treated with cranberry compositions showed a significant reduction in the copy number of CLas genes (p < 0.05), indicating the effectiveness of treating HLB-infected leaves with cranberry compositions. In Figure 5, bars representing a statistically significant difference in the reduction of CLas copy number from baseline are indicated by a star.

[00133] There was an increase in the number of copies of CLas genes in plants treated with SWE from the filter cake extract after fourteen days, but a reduction in the number of copies after twenty and Petition 870260043312, dated 08 / 05 / 2026, page 53 / 129 45 / 47 eight days. Plants treated with Filter Cake Extract SWE started with a higher number of CLa bacteria (shown at 0 hours in Figure 5) compared to the other plants. A possible explanation for the increase in CLa before the decrease in CLa is that it was more difficult for the Filter Cake Extract SWE than other types of cranberry composition to rapidly inhibit a high number of bacteria. Furthermore, the proanthocyanidins (PACs) in the Filter Cake Extract SWE are larger in size than the PACs contained in the Resin Extract and the Resin and Tanase Extract. It is possible that the Filter Cake Extract SWE took longer to enter the plant phloem than the other extract types due to the larger size of the PACs.

[00134] Figure 6 shows a change in the titration of Propidium Monoazide (PMA) from 0 hours (pre-treatment) to two days, fourteen days, and twenty-eight days for each cranberry composition treatment. The PMA titration provides an indication of the presence of live bacteria, here CLas. To quantify live bacteria, samples were treated with excess PMA. PMA selectively enters dead cells and, after photoactivation, it intercalates and covalently binds to DNA, strongly inhibiting its amplification by PCR. Therefore, mainly live DNA is amplified. A positive value for the change in PMA titration indicates a reduction in live bacteria. The predominantly negative values ​​for the change in PMA titration for water-treated plants (the control) indicate an increase in live CLas bacteria in the treated plants.Plants treated with streptomycin, resin extract, and resin extract and tannase showed a response to treatment within 48 hours, indicated by positive values ​​for the change in PMA titration. The bacterial population in plants treated with SWE of filter cake extract initially increased after forty-eight hours, as shown. Petition 870260043312, dated 08 / 05 / 2026, page 54 / 129 46 / 47 demonstrated by the negative change in PMA titer, and then began to die after 2 weeks, as demonstrated by the positive change in PMA titer. The PMA titer results are consistent with the qPCR results discussed in relation to Figure 5. Example 4: Phytotoxicity Spray Test

[00135] A small portion of a grapefruit tree was sprayed with a 10% cranberry extract. Figure 7A shows the sprayed leaves immediately after spraying, and Figure 7B shows the sprayed leaves forty-eight hours after spraying. The sprayed leaves showed no signs of phytotoxicity after forty-eight hours. The treated leaves were subsequently monitored for more than seven days (not shown in the figures) for any signs of phytotoxicity, and none were found. Example 5: Phytotoxicity Immersion Test

[00136] Citrus leaves were immersed in a 1% (1 Brix) solids solution of Filter Cake Extract (SWE) for a period of seven days. Figure 9A shows the leaves immersed on day 0 and Figure 9B shows the leaves immersed on day 7. Citrus leaves immersed in cranberry extract showed no signs of phytotoxicity after seven days. Additional citrus leaves were immersed in a control buffer solution for the same seven-day period. Figures 8A and 8B show the citrus leaves immersed in control buffer on Day 0 and Day 7, respectively. Citrus leaves immersed in control buffer showed no signs of phytotoxicity after seven days. Other Modalities

[00137] It is understood that, although the invention has been described in conjunction with the detailed description thereof, the preceding description is intended to illustrate and not limit the scope of the invention, which is Petition 870260043312, dated 08 / 05 / 2026, p. 55 / 129 47 / 47 defined by the scope of the attached claims. Other aspects, advantages, and modifications are within the scope of the following claims. Petition 870260043312, dated 08 / 05 / 2026, p. 56 / 129

Claims

1 / 2 CLAIMS 1. A method for treating or inhibiting the infection of a plant by a plant pathogen, characterized in that it comprises: contacting the plant with an amount of a composition effective in inhibiting the plant pathogen, the composition comprising phenolics from 1 mg / g to 100 mg / g, the phenolics comprising anthocyanins from 1 mg / kg to 5000 mg / kg and proanthocyanidins from 100000 mg / kg to 100000 mg / kg to treat the plant disease, quinic acid from 0.01% to 14%; and a cranberry solids content from 1 Brix to 30 Brix.

2. Method according to claim 1, characterized in that the composition comprises quinic acid at 0.05% to 14%.

3. Method according to claim 1, characterized in that the plant pathogen is α-proteobacterium Candidates liberibacter asiaticus.

4. A method according to claim 1, characterized in that the composition is in the form of a fertilizer stick, a solid fertilizer mixture, a liquid fertilizer mixture, a soil spray, a soil comprising the composition, a sprayer, a gel, an injectable composition, or an implantable composition.

5. Composition for the treatment of a plant disease, characterized in that it comprises: phenolics from 1 mg / g to 100 mg / g; wherein the phenolics comprise anthocyanins from 1 mg / kg to 5000 mg / kg and proanthocyanidins from 1,000 mg / kg to 100,000 mg / kg; quinic acid from 0.01% to 14%; a cranberry solids content from 1 Brix to 30 Brix; and Petition 870260043312, dated 08 / 05 / 2026, page. 118 / 129 2 / 2 an adjuvant that allows the composition to be administered to at least part of the phloem of a citrus plant, wherein the composition is in the form of a fertilizer spike, a solid fertilizer mixture, a liquid fertilizer mixture, a soil spray, a soil comprising the composition, a sprayer, a gel, an injectable composition, or an implantable composition.

6. Composition according to claim 5, characterized in that the adjuvant comprises a surfactant.

7. Composition, according to claim 5, characterized in that it further comprises a starch-degrading enzyme, wherein the adjuvant is configured to supply the starch-degrading enzyme to at least a portion of the plant phloem. Petition 870260043312, dated 08 / 05 / 2026, pp. 119 / 129

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