Gold nanoparticle-aptamer conjugate-based antibody delivery system, and preparation method thereof

Abstract

The present invention relates to an antibody carrier based on a gold nanoparticle (AuNP)-aptamer conjugate and a method of preparing the same. The antibody carrier according to the present invention is prepared by binding of an immunoglobulin G (IgG) Fc domain-specific aptamer or a fluorescein isothiocyanate (FITC)-specific aptamer to AuNP, and may effectively deliver an antibody into the cell nucleus, the cytoplasm and mitochondria by specific binding of the antibody to be delivered to the aptamer. In addition, since the AuNP having very low cytotoxicity is used, the antibody carrier is not only harmless to the human body, but may also detect a location in cells easily by reflecting light at various wavelengths, and therefore, it is expected to be effectively used in diagnosis or treatment of a disease.

Description

(DESCRIPTION) Invention Title GOLD NANOPARTICLE-APTAMER CONJUGATE-BASED ANTIBODY DELIVERY SYSTEM, AND PREPARATION METHOD THEREOF

Cross-Reference to Related Applications

Claims

1. An antibody carrier comprising: a gold nanoparticle (AuNP); and an aptamer binding to the surface of the AuNP, wherein the aptamer specifically binding to the Fc domain of the antibody comprises a base sequence of SEQ ID NO: 1, or wherein the aptamer specifically binding to fluorescein isothiocyanate bound to the antibody comprises a base sequence of SEQ ID NO: 2.

2. The antibody carrier of claim 1, wherein the antibody carrier delivers antibodies into cells by binding of one or more monoclonal antibodies selected from the group consisting of IgG, PARP1, VP6, NSD2, Aurora A, Pax5, Vimentin, Rock-1, Rock-2, RhoE, P53, Mcl-1, P50 and BRAF to the aptamer.

3. The antibody carrier of claim 1, wherein the AuNP has a diameter of 10 to 20 nm.

4. The antibody carrier of claim 1, wherein the antibody carrier delivers antibodies into one or more selected from the group consisting of the cell nucleus, the cytoplasm and mitochondria.

5. A method of preparing an antibody carrier, which comprises binding an aptamer to a gold nanoparticle (AuNP), wherein the aptamer specifically binding to the Fc domain of the antibody comprises a base sequence of SEQ ID NO: 1, or wherein the aptamer specifically binding to fluorescein isothiocyanate bound to the antibody comprises a base sequence of SEQ ID NO: 2.

6. A complex prepared by binding the antibody carrier of claim 1 with an antibody to be delivered.

7. A pharmaceutical composition for preventing or treating cancer, which comprises the complex of claim 6 as an active ingredient and a pharmaceutical carrier, excipient or diluent.

8. A use of the pharmaceutical composition of claim 7 to prevent or treat cancer.