Biological consortia

Abstract

The disclosure provides microbial signalers that increase the plant growth-promoting function of target microbes present in commercial biological agents. The plant-growth promoting functions may include plant pathogen inhibiting functions (e.g., nematicide functions), zinc solubilizing functions, or phosphate solubilizing functions. The disclosure further relates to compositions, comprising: the microbial signalers, and to methods of using the compositions to produce improved soil for plant growth.

Description

BIOLOGICAL CONSORTIACROSS-REFERENCE TO RELATED APPLICATOINS

[0001] This application claims the benefit of priority to U.S. Provisional Application No. 63 / 552,439, filed on February 12, 2024, and U.S. Provisional Application No. 63 / 651,760, filed on May 24, 2024. These applications are hereby incorporated by reference in their entireties for all purposes.REFERENCE TO AN ELECTRONIC SEQUENCE LISTING

[0002] The contents of the electronic sequence listing (BICL_004_02WO_SeqList_ST26.xml; Size: 86,838 bytes; and Date of Creation: February 7, 2025) are herein incorporated by reference in its entirety.BACKGROUND

[0003] Commercially available biological agents (e.g. biological control agents, biofertilizers) may be used to improve soil quality and promote plant growth, based on their ability to suppress the growth of plant pathogens, and / or increase soil nutrient availability.

[0004] However, the use of commercial biological agents has been limited by, for example, a limited base of microbes upon which development is focused and / or a focus on single-strain inoculants. In particular, single strain inoculants can fail to provide a level of plant growth promotion and / or disease suppression that is sufficient to satisfy market demands. The capacity for a single microbial strain to provide protection against any possible soil borne pathogen on diverse crops in a wide range of physical and environmental conditions, and in the presence of complex and highly-variable naturally occurring soil microbial communities, is low. For instance, many commercial biological plant pathogen control agents, such as biological pesticides, perform poorly under low nutrient conditions.

[0005] Therefore, there is a need for compositions and methods for improving the quality of soil and promoting plant growth, particularly, compositions and methods that increase the activity of commercial biological agents to suppress plant pathogens, increase soil nutrient availability, and / or enable the commercial agents to function in low nutrient environments.SUMMARY

[0006] The disclosure provides compositions, comprising at least one microbial signaler, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of a target microbe. The disclosure further provides compositions, comprising: (a) at least one microbial signaler, and (b) at least one target microbe, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of the at least one target microbe. In some embodiments, the plant growth-promoting function comprises: (a) plant pathogen-inhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, or (e) any combination thereof.

[0007] The disclosure also provides methods of method of producing a composition, the method comprising: bringing at least one target microbe in the physical proximity of at least one microbial signaler, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of the at least one target microbe, and methods of enhancing a plant growth-promoting function of a target microbe, the method comprising: bringing the target microbe in the physical proximity of at least one microbial signaler, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of the at least one target microbe.

[0008] The disclosure provides methods of producing an improved soil for plant growth, comprising: applying any one of the compositions disclosed herein to soil, thereby producing the improved soil for plant growth. In some embodiments, the methods comprise allowing a plant to grow in the improved soil. In some embodiments, the growth of the plant is more enhanced in the improved soil, as compared to the growth of the plant in a control soil, wherein the composition is not applied to the control soil.BRIEF DESCRIPTION OF THE FIGURES

[0009] FIG. 1A (upper panel) shows a culture plate showing colonies of the active microbe in Streptomyces spp. in commercial product 1 and the microbial signaler MS2 inoculated > 3 cm apart or 1 cm apart in the presence of the indicator microbe 22-D-2. The bottom panels of FIGs. 1A-1C are schematic legends showing the identity of the colonies on the plate. FIG. IB (upper panel) shows a culture plate showing colonies of the active microbe in Streptomyces spp. in commercial product 2 and the microbial signaler MS8 inoculated > 3 cm apart or 1 cm apart in the presence of the indicator microbe B3. FIG. 1C (upper panel) shows a culture plate showingcolonies of the active microbe in Streptomyces spp. in commercial product 1 and the microbial signaler MS5 inoculated > 3 cm apart or 1 cm apart in the presence of the indicator microbe 22-D-2.

[0010] FIG. 2 shows a culture plate showing colonies of the active microbe in Streptomyces spp. in commercial product 1 and the microbial signaler MS5 inoculated > 3 cm apart or 1 cm apart in the presence of the indicator microbe 33-U-4 either under low or high nutrient conditions.

[0011] FIG. 3 shows a graph depicting the inhibition zone size for Streptomyces spp. in commercial product 1 against the indicator microbes 33-U-4 or B3 under high or low nutrient conditions.

[0012] FIGs. 4A-4B show a graph depicting an increase in inhibition zone for Streptomyces spp. in commercial product 1 against the indicator microbes 33-U-4 (FIG. 4A) or B3 (FIG. 4B) under high nutrient conditions or low nutrient conditions in the presence of the each of the microbial signalers as indicated.

[0013] FIG. 5A depicts the percentage increase in the inhibition of five indicator microbes (Bacillus spp.) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1, as compared to the inhibition of the indicator microbe (Bacillus spp.) in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 5B depicts the percentage increase in the inhibition of an indicator microbe (Bacillus spp. (22-D2)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1, as compared to the inhibition of the indicator microbe (Bacillus spp. (22-D2)) in the presence of Streptomyces spp. In commercial product 1 alone. FIG. 5C depicts the percentage increase in the inhibition of an indicator microbe (Bacillus spp. (33-U-4)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1 , as compared to the inhibition of the indicator microbe (Bacillus spp. (33-U-4)) in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 5D depicts the percentage increase in the inhibition of an indicator microbe (Bacillus spp. (B3)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. In commercial product 1, as compared to the inhibition of the indicator microbe (Bacillus spp. (B3)) in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 5E depicts the percentage increase in the inhibition of an indicator microbe(Bacillus spp. (62-D-2)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1 , as compared to the inhibition of the indicator microbe (Bacillus spp. (62-D-2)) in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 5F depicts the percentage increase in the inhibition of a plant pathogen (Streptomyces scabies) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1, as compared to the inhibition of the plant pathogen (Streptomyces scabies) in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 5G depicts the percentage increase in the inhibition of an indicator microbe (Bacillus spp. (52-U-l)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1 , as compared to the inhibition of the indicator microbe (Bacillus spp. (52-U-l)) in the presence of Streptomyces spp. in commercial product 1 alone.

[0014] FIG. 6A depicts the percentage increase in the inhibition of a plant pathogen (Colletotrichum graminicola) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) target microbe Bacillus spp. , as compared to the inhibition of the plant pathogen (Colletotrichum graminicola) in the presence of Bacillus spp. alone. FIG. 6B depicts the percentage increase in the inhibition of a plant pathogen (Pectobacterium caratovorum) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) target microbe Bacillus spp., as compared to the inhibition of the plant pathogen (Pectobacterium caratovorum) in the presence of target microbe Bacillus spp. alone.

[0015] FIG. 7A depicts the percentage increase in the inhibition of a plant pathogen (Rhizoctonia solani) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) target microbe Bacillus spp., as compared to the inhibition of the plant pathogen (Rhizoctonia solani) in the presence of target microbe Bacillus spp. alone. FIG. 7B depicts the percentage increase in the inhibition of a plant pathogen (Sclerotinia sclerotiorum) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) target microbe Bacillus spp., as compared to the inhibition of the plant pathogen (Sclerotinia sclerotiorum) in the presence of target microbe Bacillus spp. alone.

[0016] FIG. 8A depicts the percentage increase in the inhibition of an indicator microbe (Bacillus spp. (22-D2)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. In commercial product 2, as compared to the inhibition of the indicator microbe (Bacillus spp. (22-D2)) in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 8B depicts the percentage increase in the inhibition of anindicator microbe (Bacillus spp. (B3, 22-D2)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the inhibition of the indicator microbe (Bacillus spp. (B3, 22-D2)) in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 8C depicts the percentage increase in the inhibition of an indicator microbe (Bacillus spp. (B3)) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the inhibition of the indicator microbe (Bacillus spp. (B3)) in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 8D depicts the percentage increase in the inhibition of a plant pathogen (Pythium irregulare) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the inhibition of the plant pathogen (Pythium irregulare) in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 8E depicts the percentage increase in the inhibition of a plant pathogen (Rhizoctonia solani) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the inhibition of the plant pathogen (Rhizoctonia solani) in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 8F depicts the percentage increase in the inhibition of a plant pathogen (Streptomyces scabies) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the inhibition of the plant pathogen (Streptomyces scabies) in the presence of Streptomyces spp. in commercial product 2 alone.

[0017] FIG. 9 depicts the percentage increase in the inhibition of a plant pathogen (Streptomyces scabies) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Bacillus spp. in a commercial product, as compared to the inhibition of the plant pathogen (Streptomyces scabies) in the presence of Bacillus spp. in a commercial product alone.

[0018] FIG. 10A depicts the percentage increase in the inhibition of a plant pathogen (Fusarium culmorum) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Talaromyces spp. in a commercial product , as compared to the inhibition of the plant pathogen (Fusarium culmorum) in the presence of Talaromyces spp. in a commercial product alone. FIG. 10B depicts the percentage increase in the inhibition of a plant pathogen (Fusarium graminearum) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Talaromyces spp. in a commercial product , ascompared to the inhibition of the plant pathogen (Fusarium graminearum) in the presence of Talaromyces spp. in a commercial product alone. FIG. IOC depicts the percentage increase in the inhibition of a plant pathogen (Fusarium oxysporum) in the presence of a combination of:(a) each of the microbial signalers listed on the X axis, and (b) Talaromyces spp. in a commercial product, as compared to the inhibition of the plant pathogen (Fusarium oxysporum) in the presence of Talaromyces spp. in a commercial product alone. FIG. 10D depicts the percentage increase in the inhibition of a plant pathogen (Pythium irregulare) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Talaromyces spp. in a commercial product , as compared to the inhibition of the plant pathogen (Pythium irregulare) in the presence of Talaromyces spp. in a commercial product alone. FIG. 10E depicts the percentage increase in the inhibition of a plant pathogen (Rhizoctonia solani) in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and(b) Talaromyces spp. in a commercial product , as compared to the inhibition of the plant pathogen (Rhizoctonia solani) in the presence of Talaromyces spp. in a commercial product alone.

[0019] FIG. 11A depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1, as compared to the phosphate solubilization in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 11B depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Pseudomonas spp.; Comamonas spp.; Citrobacter spp. ; and Enterobacter spp. in a commercial product, as compared to the phosphate solubilization in the presence of Pseudomonas spp.; Comamonas spp.; Citrobacter spp.; and Enterobacter spp. in a commercial product alone. FIG. 11C depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the phosphate solubilization in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 11D depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Trichoderma spp. in a commercial product, as compared to the phosphate solubilization in the presence of Trichoderma spp. in a commercial product alone. FIG. HE depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Bacillus spp. in a commercial product, ascompared to the phosphate solubilization in the presence of Bacillus spp. in a commercial product alone. FIG. 11F depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Talaromyces spp. in a commercial product , as compared to the phosphate solubilization in the presence of Talaromyces spp. in a commercial product alone.

[0020] FIG. 12A depicts the percentage increase in the zinc solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 1, as compared to the zinc solubilization in the presence of Streptomyces spp. in commercial product 1 alone. FIG. 12B depicts the percentage increase in the zinc solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Streptomyces spp. in commercial product 2, as compared to the zinc solubilization in the presence of Streptomyces spp. in commercial product 2 alone. FIG. 12C depicts the percentage increase in the zinc solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Trichoderma spp. in a commercial product, as compared to the zinc solubilization in the presence of Trichoderma spp. in a commercial product alone. FIG. 12D depicts the percentage increase in the zinc solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) Talaromyces spp. in a commercial product, as compared to the zinc solubilization in the presence of Talaromyces spp. in a commercial product alone.

[0021] FIG. 13A depicts the percentage increase in the inhibition of Fusarium culmorum as measured by Assay 1 described in Example 3 in the presence of a combination of: (a) the microbial signaler listed on the X axis, and (b) a Trichoderma spp. microbe in a commercial product, as compared to the inhibition of the plant pathogen in the presence of the Trichoderma spp. microbe in the commercial product alone. FIG. 13B depicts the percentage increase in the inhibition of Fusarium culmorum as measured by Assay 2 described in Example 3 in the presence of a combination of: (a) the microbial signaler listed on the X axis, and (b) a Trichoderma spp. microbe in a commercial product, as compared to the inhibition of the plant pathogen in the presence of the Trichoderma spp. microbe in the commercial product alone.

[0022] FIG. 14A depicts the percentage increase in the inhibition of a plant pathogen (Phytophthora sojae) as measured by Assay 1 in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) a Trichoderma spp. microbe in a commercial product, as compared to the inhibition of the plant pathogen (Phytophthora sojae) in the presence of the Trichoderma spp. microbe in the commercial product on its own. FIG.14B depicts the percentage increase in the inhibition of a plant pathogen (Phytophthora sojae) as measured by Assay 2 in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) a Trichoderma spp. microbe in a commercial product, as compared to the inhibition of the plant pathogen (Phytophthora sojae) in the presence of the Trichoderma spp. microbe in the commercial product on its own.

[0023] FIG. 15A depicts the percentage increase in Phytophthora sojae disease suppression (wherein a reduction in disease incidence is measured based on a reduction in the proportion of infected plants) on soybean plants inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Trichoderma spp. microbe in a commercial product, as compared to the Trichoderma spp. microbe in the commercial product on its own. FIG. 15B depicts the percentage increase in Phytophthora sojae disease suppression (wherein a reduction in disease severity is assessed on a scale of 1 through 5) on soybean plants inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Trichoderma spp. microbe in a commercial product, as compared to the Trichoderma spp. microbe in the commercial product on its own.

[0024] FIG. 16A depicts the percentage increase in aboveground biomass of corn inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Azospirillum spp. microbe in a commercial product, as compared to the Azospirillum spp. microbe in the commercial product on its own, or just water. FIG. 16B depicts the percentage increase in corn plants reaching vegetative growth stage 4 (V4), which were inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Azospirillum spp. microbe in a commercial product, as compared to the Azospirillum spp. microbe in the commercial product on its own or just water.

[0025] FIG. 17A depicts the percentage increase in aboveground biomass of soybean inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Bradyrhizobium spp. microbe in a commercial product, as compared to the Bradyrhizobium spp. microbe in the commercial product on its own. FIG. 17B depicts the percentage increase in aboveground biomass of soybean inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Bradyrhizobium spp. microbe in a commercial product, as compared to the Bradyrhizobium spp. microbe in the commercial product on its own.

[0026] FIG. 18A depicts the percentage increase in belowground biomass of com plants inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Glomus spp.in a mycorrhizal commercial product, as compared to the Glomus spp. in a mycorrhizal commercial product on its own or just water. FIG. 18B depicts the percentage increase in average growth stage among soybean plants inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Glomus spp. in a mycorrhizal commercial product, as compared to the Glomus spp. in a mycorrhizal commercial product on its own.

[0027] FIG. 19 is an image of com plants inoculated with: (a) the indicated microbial signaling isolate, and (b) a Glomus spp. in a mycorrhizal commercial product, as compared to the Glomus spp. in a mycorrhizal commercial product on its own. The addition of the microbial signaling isolate enhances plant vigor and reduces purple coloration associated with nutrient stress.

[0028] FIG. 20A depicts the percentage increase in fresh belowground biomass of corn plants inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Bacillus spp. in a commercial product, as compared to the Bacillus spp. in a commercial product on its own or just water. FIG. 20B depicts the percentage increase in dry aboveground biomass of soybean plants inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) aBacillus spp. in a commercial product, as compared to the Bacillus spp. in a commercial product on its own or just water. FIG. 20C depicts the percentage increase in frequency of healthy unifoliate leaves of soybean inoculated with: (a) each of the microbial signalers listed on the X axis, and (b) a Bacillus spp. in a commercial product, as compared to the Bacillus spp. in a commercial product on its own or just water.

[0029] FIG. 21 shows images of soybean plants inoculated with: (a) the indicated microbial signaler listed, and (b) a Bacillus spp. in a commercial product, as compared to the Bacillus spp. in a commercial product on its own or just water.

[0030] FIG. 22 depicts the percentage increase in in vitro phosphate solubilization by Pseudomonas spp. ; Comamonas spp. ; Citrobacter spp. ; and Enterobacter spp. in a commercial product at three different inoculum densities (lx undiluted, lOx diluted, lOOx diluted) in presence of a microbial signaler disclosed herein, JBS9225.

[0031] FIG. 23 depicts the percentage reduction in Pythium disease severity in plants inoculated with: (a) a combination of microbial signalers JBS4783, JBS8135, JBS3880, and (b) a Talaromyces spp. target microbe in a commercial product, as compared to the Talaromyces spp. target microbe in a commercial product on its own.

[0032] FIG. 24 depicts the percentage increase in the phosphate solubilization in the presence of a combination of: (a) each of the microbial signalers listed on the X axis, and (b) aTalaromyces spp. target microbe in a commercial product, as compared to the phosphate solubilization in the presence of ) a Talaromyces spp. target microbe in a commercial product alone.

[0033] FIG. 25 depicts the percentage increase in the inhibition of Fusarium culmorum in the presence of a combination of: (a) JBS6226, and (b) a Bacillus spp. microbe in a commercial product, as compared to the inhibition of the plant pathogen in the presence of the Bacillus spp. microbe in the commercial product alone.

[0034] FIGs. 26A-26B depict exemplary instrumentation (FIG. 26A) used to evenly coat seeds with formulations comprising microbial isolates (JBS) and commercial product (FIG.26B)

[0035] FIG. 27A-27B depict photographs providing exemplary differences between untreated seeds (TRT 1) and seeds treated with microbial product, base, and microbial signaler JBS3880 (TRT 7) when assessed for seed germination and plantability.

[0036] FIG. 28 depicts exemplary US locations used to trial field-readiness and ability to support areas with limited field trialing capacities of microbial signalers: Ames, Iowa; Lakeview, Iowa; Atlantic, Iowa; Geneseo, Illinois; Beresford, South Dakota; Jackson, Minnesota; Mendota, Illinois; Nevada, Iowa; Volga, South Dakota, and; Austin, Minnesota.

[0037] FIGs. 29A-29D depict photographs providing exemplary differences between seeds and resultant plant products treated with commercial microbial product and base (i.e., TRT #3) versus commercial microbial product, base, and microbial signaler JBS9311 (TRT #6) at two locations: Mendota, IL (FIGs. 29A and 29B, respectively) and Atlantic, IA (FIGs. 29C and 29D, respectively).

[0038] FIG. 30 depicts a photograph providing exemplary differences in nodulization between seeds and resultant plant products untreated (TRT #1), treated with commercial microbial product and base (TRT #3), or commercial microbial product, base, and microbial signaler JBS9311 (TRT #6).

[0039] FIGs. 31A-31B provides assessments of treatment groups #l-#7 at mid-season for plant vegetation (ND VI; FIG. 31A) and plant health (NDRE; FIG. 31B).

[0040] FIG. 32 provides assessments of average yield time in bushels per acre (bu / acre) by location at harvest time. Locations are as in FIG. 28. Locations were categorized into highyielding location (YLD) and low YLD based on calculated yield found to be higher or lower than 55 bu / acre average yield across all locations, respectively.

[0041] FIG. 33 provides assessments of yield in bushels per acre (bu / acre) by treatment groups (TRT #4-#8) at harvest time. Percent differences are relative to commercial product control (TRT #3).

[0042] FIG. 34 provides assessments of yield average in bushels per acre (bu / acre) by treatment groups (TRT #4-8) separated into high yielding locations (YLD) and low YLD. Yield was calculated at harvest time.DETAILED DESCRIPTIONDefinitions

[0043] While the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to facilitate explanation of the presently disclosed subject matter.

[0044] The term “a” or “an” may refer to one or more of that entity, i.e. can refer to plural referents. As such, the terms “a” or “an”, “one or more” and “at least one” are used interchangeably herein. In addition, reference to “an element” by the indefinite article “a” or “an” does not exclude the possibility that more than one of the elements is present, unless the context clearly requires that there is one and only one of the elements. For example, “at least one target microbe” is interchangeable with “a target microbe”.

[0045] Reference throughout this specification to “one embodiment”, “an embodiment”, “one aspect”, or “an aspect” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present disclosure. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics can be combined in any suitable manner in one or more embodiments.

[0046] As used herein, in particular embodiments, the terms “about” or “approximately” when preceding a numerical value indicates the value plus or minus a range of 10% unless otherwise stated or otherwise evident by the context, and except where such a range would exceed 100% of a possible value, or fall below 0% of a possible value, such as less than 0 CFU / ml of a bacteria, or more than 100% of a inhibition of growth.

[0047] As used herein the terms “microorganism” or “microbe” should be taken broadly. These terms are used interchangeably and include, but are not limited to, the two prokaryotic domains, Bacteria and Archaea, eukaryotic fungi and protozoa, as well as viruses.

[0048] The term “microbial community” means a group of microbes comprising two or more species or strains. Unlike microbial consortia, a microbial community does not have to be carrying out a common function, or does not have to be participating in, or leading to, or correlating with, a recognizable parameter, such as a phenotypic trait of interest (e.g. antimicrobial activity or production of compounds beneficial to plant growth).

[0049] As used herein, “isolate,” “isolated,” “isolated microbe,” and like terms, are intended to mean that the one or more microorganisms has been separated from at least one of the materials with which it is associated in a particular environment (for example soil, water, plant tissue).

[0050] As used herein, “soil” refers to any plant growth medium including any agriculturally acceptable growing media. Growing media may include, for example, soil, sand, compost, peat, soilless growing media containing organic and / or inorganic ingredients, artificial plant-growth substrates, polymer-based growth matrices, hydroponic nutrient and growth solutions, and combinations or mixtures thereof.

[0051] Microbes of the present disclosure may include spores and / or vegetative cells. In some embodiments, microbes of the present disclosure include microbes in a viable but non- culturable (VBNC) state, or a quiescent state. See Liao and Zhao (US Publication US2015267163A1). In some embodiments, microbes of the present disclosure include microbes in a biofilm. See Merritt et al. (U.S. Patent 7,427,408).

[0052] Thus, an “isolated microbe” does not exist in its naturally occurring environment; rather, it is through the various techniques described herein that the microbe has been removed from its natural setting and placed into a non-naturally occurring state of existence. Thus, the isolated strain or isolated microbe may exist as, for example, a biologically pure culture, or as spores (or other forms of the strain) in association with an acceptable carrier.

[0053] As used herein, “spore” or “spores” refer to structures produced by bacteria and fungi that are adapted for survival and dispersal. Spores are generally characterized as dormant structures; however, spores are capable of differentiation through the process of germination. Germination is the differentiation of spores into vegetative cells that are capable of metabolic activity, growth, and reproduction. The germination of a single spore results in a single fungalor bacterial vegetative cell. Fungal spores are units of asexual reproduction, and in some cases are necessary structures in fungal life cycles. Bacterial spores are structures for surviving conditions that may ordinarily be nonconductive to the survival or growth of vegetative cells.

[0054] As used herein, “microbial composition” refers to a composition comprising one or more microbes or one or more microbial signalers of the present disclosure, wherein a microbial composition, in some embodiments, is administered to the soil, field, or plants described herein.

[0055] As used herein, “carrier”, “acceptable carrier”, or “agricultural carrier” refers to a diluent, adjuvant, excipient, or vehicle with which the compound is administered.

[0056] In some embodiments, carriers may be granular in structure, such as soil, sand, soil particles, or sand particles. In further embodiments, the carriers may be dry, as opposed to a moist or wet carrier. In some embodiments, carriers can be in solid or liquid form.

[0057] The terms “multi strain inoculate composition”, “consortium”, “bioconsortia,” “microbial consortia,” and “synthetic consortia” interchangeably refer to a composition comprising two or more microbes. In some embodiments, the microbes in the consortium do not exist together in a naturally occurring environment. In some embodiments, the microbes are present in the consortium at ratios or amounts that are not naturally occurring. In some embodiments, the consortium comprises two or more species, or two or more strains of a species, of microbes.

[0058] In certain embodiments of the disclosure, the isolated microbes exist as isolated and biologically pure cultures (e.g., microbial isolate(s)). It will be appreciated by one of skill in the art, that an isolated and biologically pure culture of a particular microbe, denotes that said culture is substantially free (within scientific reason) of other living organisms and contains only the individual microbe in question. The culture can contain varying concentrations of said microbe. The present disclosure notes that isolated and biologically pure microbes often “necessarily differ from less pure or impure materials.” See, e.g. In re Bergstrom, 427 F.2d 1394, (CCPA 1970)(discussing purified prostaglandins), see also, In re Bergy, 596 F.2d 952 (CCPA 1979)(discussing purified microbes), see also, Parke-Davis & Co. v. H.K. Mulford & Co., 189 F. 95 (S.D.N.Y. 1911) (Learned Hand discussing purified adrenaline), aff’d in part, rev’d in part, 196 F. 496 (2d Cir. 1912), each of which are incorporated herein by reference. Furthermore, in some embodiments, the disclosure provides for certain quantitative measures of the concentration, or purity limitations, that must be found within an isolated andbiologically pure microbial culture. The presence of these purity values, in certain embodiments, is a further attribute that distinguishes the presently disclosed microbes from those microbes existing in a natural state. See, e.g., Merck & Co. v. Olin Mathieson Chemical Corp., 253 F.2d 156 (4thCir. 1958) (discussing purity limitations for vitamin B12 produced by microbes), incorporated herein by reference.

[0059] As used herein, “individual isolates” should be taken to mean a composition, or culture, comprising a predominance of a single genera, species, or strain, of microorganism, following separation from one or more other microorganisms. The phrase should not be taken to indicate the extent to which the microorganism has been isolated or purified. However, “individual isolates” can comprise substantially only one genus, species, or strain, of microorganism.

[0060] The term “growth medium” as used herein, is any medium which is suitable to support growth of a microbe. By way of example, the media may be natural or artificial. It should be appreciated that the media may be used alone or in combination with one or more other media. It may also be used with or without the addition of exogenous nutrients.

[0061] The medium may be amended or enriched with additional compounds or components, for example, a component which may assist in the interaction and / or selection of specific groups of microorganisms. For example, antibiotics (such as penicillin) or sterilants (for example, quaternary ammonium salts and oxidizing agents) could be present and / or the physical conditions (such as salinity, nutrients (for example organic and inorganic minerals (such as phosphorus, nitrogenous salts, ammonia, potassium and micronutrients such as cobalt and magnesium), Ph, and / or temperature), methionine, prebiotics, ionophores, and beta glucans could be amended.

[0062] As used herein, “improved” or “enhanced” should be taken broadly to encompass improvement of a characteristic of interest, as compared to a control group, or as compared to a known average quantity associated with the characteristic in question. In the present disclosure, “improved” does not necessarily demand that the data be statistically significant (i.e. p < 0.05); rather, any quantifiable difference demonstrating that one value (e.g. the average treatment value) is different from another (e.g. the average control value) can rise to the level of “improved.”

[0063] As used herein, “inhibiting” and “suppressing” are used interchangeably and these and other like terms should not be construed to require complete inhibition or suppression, although this may be desired in some embodiments.

[0064] The term “marker” or “unique marker” as used herein is an indicator of unique microorganism type, microorganism strain or activity of a microorganism strain. A marker can be measured in biological samples and includes without limitation, a nucleic acid-based marker such as a ribosomal RNA gene, a peptide- or protein-based marker, and / or a metabolite or other small molecule marker.

[0065] The term “metabolite” as used herein is an intermediate or product of metabolism. A metabolite in one embodiment is a small molecule. Metabolites have various functions, including in fuel, structural, signaling, stimulatory and inhibitory effects on enzymes, as a cofactor to an enzyme, in defense, and in interactions with other organisms (such as pigments, odorants and pheromones). A primary metabolite is directly involved in normal growth, development and reproduction. A secondary metabolite is not directly involved in these processes but usually has an important ecological function. Examples of metabolites include but are not limited to antibiotics and pigments such as resins and terpenes, etc. Some antibiotics use primary metabolites as precursors, such as actinomycin which is created from the primary metabolite, tryptophan. Metabolites, as used herein, include small, hydrophilic carbohydrates; large, hydrophobic lipids and complex natural compounds.

[0066] As used herein, the term “genotype” refers to the genetic makeup of an individual cell, cell culture, tissue, organism, or group of organisms.

[0067] As used herein, the term “allele(s)” means any of one or more alternative forms of a gene, all of which alleles relate to at least one trait or characteristic. In a diploid cell, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes. Since the present disclosure, in embodiments, relates to QTLs, i.e. genomic regions that may comprise one or more genes or regulatory sequences, it is in some instances more accurate to refer to “haplotype” (i.e. an allele of a chromosomal segment) instead of “allele”, however, in those instances, the term “allele” should be understood to comprise the term “haplotype”. Alleles are considered identical when they express a similar phenotype. Differences in sequence are possible but not important as long as they do not influence phenotype.

[0068] As used herein, the term “locus” (loci plural) means a specific place or places or a site on a chromosome where for example a gene or genetic marker is found.

[0069] As used herein, the term “genetically linked” refers to two or more traits that are coinherited at a high rate during breeding such that they are difficult to separate through crossing.

[0070] A “recombination” or “recombination event” as used herein refers to a chromosomal crossing over or independent assortment. The term “recombinant” refers to an organism having a new genetic makeup arising as a result of a recombination event.

[0071] As used herein, the term “molecular marker” or “genetic marker” refers to an indicator that is used in methods for visualizing differences in characteristics of nucleic acid sequences. Examples of such indicators are restriction fragment length polymorphism (RFLP) markers, amplified fragment length polymorphism (AFLP) markers, single nucleotide polymorphisms (SNPs), insertion mutations, microsatellite markers (SSRs), sequence-characterized amplified regions (SCARs), cleaved amplified polymorphic sequence (CAPS) markers or isozyme markers or combinations of the markers described herein which defines a specific genetic and chromosomal location. Markers further include polynucleotide sequences encoding 16S or 18S Rma, and internal transcribed spacer (ITS) sequences, which are sequences found between small-subunit and large-subunit Rma genes that have proven to be especially useful in elucidating relationships or distinctions among when compared against one another. Mapping of molecular markers in the vicinity of an allele is a procedure which can be performed by the average person skilled in molecular-biological techniques.

[0072] The primary structure of major Rma subunit 16S comprise a particular combination of conserved, variable, and hypervariable regions that evolve at different rates and enable the resolution of both very ancient lineages such as domains, and more modern lineages such as genera. The secondary structure of the 16S subunit include approximately 50 helices which result in base pairing of about 67% of the residues. These highly conserved secondary structural features are of great functional importance and can be used to ensure positional homology in multiple sequence alignments and phylogenetic analysis. Over the previous few decades, the 16S Rma gene has become the most sequenced taxonomic marker and is the cornerstone for the current systematic classification of bacteria and archaea (Yarza et al. 2014. Nature Rev. Micro. 12:635-45).

[0073] A sequence identity of 94.5% or lower for two 16S Rrna genes is strong evidence for distinct genera, 86.5% or lower is strong evidence for distinct families, 82% or lower is strong evidence for distinct orders, 78.5% is strong evidence for distinct classes, and 75% or lower is strong evidence for distinct phyla. The comparative analysis of 16S Rrna gene sequences enables the establishment of taxonomic thresholds that are useful not only for the classification of cultured microorganisms but also for the classification of the many environmental sequences. Yarza et al. 2014. Nature Rev. Micro. 12:635-45).

[0074] As used herein, the term “trait” refers to a characteristic or phenotype. A trait may be inherited in a dominant or recessive manner, or in a partial or incomplete-dominant manner. A trait may be monogenic (i.e. determined by a single locus) or polygenic (i.e. determined by more than one locus) or may also result from the interaction of one or more genes with the environment.

[0075] As used herein, the term “phenotype” refers to the observable characteristics of an individual cell, cell culture, organism (e.g., a bacterium), or group of organisms which results from the interaction between that individual’s genetic makeup (z.e., genotype) and the environment.

[0076] As used herein, the term “chimeric” or “recombinant” when describing a nucleic acid sequence or a protein sequence refers to a nucleic acid, or a protein sequence, that links at least two heterologous polynucleotides, or two heterologous polypeptides, into a single macromolecule, or that re-arranges one or more elements of at least one natural nucleic acid or protein sequence. For example, the term “recombinant” can refer to an artificial combination of two otherwise separated segments of sequence, e.g., by chemical synthesis or by the manipulation of isolated segments of nucleic acids by genetic engineering techniques.

[0077] As used herein, a “synthetic nucleotide sequence” or “synthetic polynucleotide sequence” is a nucleotide sequence that is not known to occur in nature or that is not naturally occurring. Generally, such a synthetic nucleotide sequence will comprise at least one nucleotide difference when compared to any other naturally occurring nucleotide sequence.

[0078] As used herein, the term “nucleic acid” refers to a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides, or analogs thereof. This term refers to the primary structure of the molecule, and thus includes double- and single-stranded DNA, as well as double- and single-stranded RNA. It also includes modified nucleic acids such as methylated and / or capped nucleic acids, nucleic acids containing modified bases, backbone modifications, and the like. The terms “nucleic acid” and “nucleotide sequence” are used interchangeably.

[0079] As used herein, the term “gene” refers to any segment of DNA associated with a biological function. Thus, genes include, but are not limited to, coding sequences and / or the regulatory sequences required fortheir expression. Genes can also include non-expressed DNA segments that, for example, form recognition sequences for other proteins. Genes can be obtained from a variety of sources, including cloning from a source of interest or synthesizingfrom known or predicted sequence information, and may include sequences designed to have desired parameters.

[0080] As used herein, the term “homologous” or “homologue” or “ortholog” is known in the art and refers to related sequences that share a common ancestor or family member and are determined based on the degree of sequence identity. The terms “homology,” “homologous,” “substantially similar” and “corresponding substantially” are used interchangeably herein. They refer to nucleic acid fragments wherein changes in one or more nucleotide bases do not affect the ability of the nucleic acid fragment to mediate gene expression or produce a certain phenotype. These terms also refer to modifications of the nucleic acid fragments of the instant disclosure such as deletion or insertion of one or more nucleotides that do not substantially alter the functional properties of the resulting nucleic acid fragment relative to the initial, unmodified fragment. It is therefore understood, as those skilled in the art will appreciate, that the disclosure encompasses more than the specific exemplary sequences. These terms describe the relationship between a gene found in one species, subspecies, variety, cultivar or strain and the corresponding or equivalent gene in another species, subspecies, variety, cultivar or strain. For purposes of this disclosure homologous sequences are compared. “Homologous sequences” or “homologues” or “orthologs” are thought, believed, or known to be functionally related. A functional relationship may be indicated in any one of a number of ways, including, but not limited to: (a) degree of sequence identity and / or (b) the same or similar biological function. Preferably, both (a) and (b) are indicated. Homology can be determined using software programs readily available in the art, such as those discussed in Current Protocols in Molecular Biology (F.M. Ausubel etal., eds., 1987) Supplement 30, section 7.718, Table 7.71. Some alignment programs are MacVector (Oxford Molecular Ltd, Oxford, U.K.), ALIGN Plus (Scientific and Educational Software, Pennsylvania) and AlignX (Vector NTI, Invitrogen, Carlsbad, CA). Another alignment program is Sequencher (Gene Codes, Ann Arbor, Michigan), using default parameters.

[0081] The term “primer” as used herein refers to an oligonucleotide which is capable of annealing to the amplification target allowing a DNA polymerase to attach, thereby serving as a point of initiation of DNA synthesis when placed under conditions in which synthesis of primer extension product is induced, z.e., in the presence of nucleotides and an agent for polymerization such as DNA polymerase and at a suitable temperature and Ph. The (amplification) primer is preferably single stranded for maximum efficiency in amplification. Preferably, the primer is an oligodeoxyribonucleotide. The primer must be sufficiently long toprime the synthesis of extension products in the presence of the agent for polymerization. The exact lengths of the primers will depend on many factors, including temperature and composition (A / T vs. G / C content) of primer. A pair of bi-directional primers consists of one forward and one reverse primer as commonly used in the art of DNA amplification such as in PCR amplification.

[0082] In some embodiments, the cell or organism has at least one heterologous trait. As used herein, the term “heterologous trait” refers to a phenotype imparted to a transformed host cell or transgenic organism by an exogenous DNA segment, heterologous polynucleotide or heterologous nucleic acid. These results can be achieved by providing expression of heterologous products or increased expression of endogenous products in organisms using the methods and compositions of the present disclosure.

[0083] As used herein “shelf-stable” refers to a functional attribute and new utility acquired by the microbes formulated according to the disclosure, which enable said microbes to exist in a useful / active state outside of their natural environment in a plant or soil (i.e. a markedly different characteristic). Thus, shelf-stable is a functional attribute created by the formulations / compositions of the disclosure and denoting that the microbe formulated into a shelf-stable composition can exist under ambient conditions for a period of time that can be determined depending upon the particular formulation utilized, but in general means that the microbes can be formulated to exist in a composition that is stable under ambient conditions for at least a few days and generally at least one week. Accordingly, a “shelf-stable soil treatment” is a composition comprising one or more microbes of the disclosure, said microbes formulated in a composition, such that the composition is stable under ambient conditions for at least one week.

[0084] As used herein, a “microbial signaler” or a “signaling microbe” refers to any microbe that has the capability to influence or alter a characteristic or function of a second microbe (referred to herein, as a “target microbe” or a “signaled microbe”) which is in its physical proximity. As used herein, “physical proximity” refers to a distance at which a “microbial signaler” is able to influence or alter a characteristic or function of the second microbe (or the target microbe). In some embodiments, the distance is less than around 3 cm. In some embodiments, the microbial signaler is adjacent (at a distance of less than or equal to around 1 cm) to the target microbe.

[0085] In some embodiments, the microbial signaler has the ability to enhance a plant growthpromoting function of the target microbe. As used herein, the “plant growth promoting function” refers to the ability of a microbe (e.g. a microbial signaler, a target microbe, or a combination thereof) to enhance the growth of a plant. The growth of the plant may be reflected by the height of the plant, the yield of the plant, disease resistance, or any combination thereof.

[0086] In some embodiments, the plant growth-promoting function comprises one or more of the following functions: (a) plant pathogen-inhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, (e) nitrogen fixation, (f) nutrient acquisition, (g) production of plant growth hormones or (e) any combination thereof. In some embodiments, the microbial signaler influences or alters the characteristic or function of the target microbe through the use of chemical, physical and / or biological signaling moi eties. In some embodiments, the microbial signaler and the target microbe are in contact with each other. In some embodiments, the microbial signaler and the target microbe are part of the same composition, such as, a composition disclosed herein.

[0087] As used herein, “bioavailability” refers to a form of an element or a compound that is accessible to an organism (e.g. a plant) for uptake, adsorption, and / or absorption. In some embodiments, bioavailable forms of an element or a compound are soluble forms of an element or a compound (e.g. zinc, or phosphate).

[0088] As used herein, “zinc solubilization” refers to the process by which non-bioavailable form of zinc is converted into a bioavailable form of zinc. In some embodiments, the non- bioavailable form of zinc is an insoluble form of zinc. In some embodiments, the bioavailable form of zinc is the soluble form of zinc. In some embodiments, zinc solubilization occurs in the soil, converting an insoluble form of zinc in the soil to a soluble form that is bioavailable for organisms (e.g. plants). Microbes that are capable of promoting or causing zinc solubilization are referred to herein as “zinc-solubilizing microbes”. Zinc solubilization may depend on a variety of factors, such as, Ph of the soil, soil moisture, soil temperature, the presence of zinc solubilizing microbes, or any combination thereof.

[0089] As used herein, “phosphate solubilization” refers to the process by which non- bioavailable form of phosphate is converted into a bioavailable form of phosphate. In some embodiments, the non-bioavailable form of phosphate is an insoluble form of phosphate. In some embodiments, the bioavailable form of phosphate is the soluble form of phosphate. In some embodiments, phosphate solubilization occurs in the soil, converting an insoluble formof phosphate in the soil to a soluble form that is bioavailable for organisms (e.g. plants). Microbes that are capable of promoting or causing phosphate solubilization are referred to herein as “phosphate-solubilizing microbes”. Phosphate solubilization may depend on a variety of factors, such as, Ph of the soil, soil moisture, soil temperature, the presence of phosphate solubilizing microbes, or any combination thereof.

[0090] As used herein, “nutrient acquisition” refers to the ability and / or process by which a plant acquires or uptakes growth-promoting nutrients, such as phosphate, zinc and other nutrients. In some embodiments, the disclosed microbial signalers, the disclosed target microbes, or any combinations thereof enhance the nutrient acquisition of a plant, thereby promoting plant growth. Enhancing nutrient acquisition may be affected by a variety of ways, for example, through improved phosphate solubilization and / or improved zinc solubilization, as described herein.

[0091] As used herein, a “high nutrient” condition, environment, medium, or soil refers to the condition, environment, medium, or soil comprising nutrients at a concentration that is in the standard range for a particular microbe. The standard nutrient concentration for a particular microbe can be ascertained by a person of ordinary skill in the art. As used herein, a “low nutrient” condition, environment, medium, or soil refers to a condition, environment, medium, or soil which comprises a concentration of nutrients that is lower than what is considered standard for that particular microbe. For example, the concentration of nutrients in the “low nutrient” condition may be half, l / 3rd, k?11, l / 5th, l / 6th, l / 7th, l / 8th, l / 9th, 1 / 10th, 1 / 15th, l / 20th, or l / 50thof the standard concentration of nutrients for that particular microbe. In some embodiments, the low nutrient condition is unable to support the pathogen-suppressing and / or antibiotic producing activities of a microbe that is known to possess pathogen-suppressing and / or antibiotic production activities. In some embodiments, the high nutrient condition is a condition under which a microbe that is known to possess pathogen-suppressing and / or antibiotic production activities exhibits the pathogen-suppressing and / or antibiotic production activities.Compositions Comprising Microbial Signalers Disclosed Herein

[0092] The disclosure provides microbial signalers that increase the performance of commercial biological agents, such as target microbes present in target commercial products. Additionally, the microbial signalers disclosed herein reduce variation in the performance ofthese target commercial products across of areas of application, such as areas that differ in nutrient availability.

[0093] The microbial signalers disclosed herein are capable of enhancing the plant growthpromoting function of one or more target microbes. For instance, the microbial signalers disclosed herein are capable of enhancing the plant pathogen-inhibiting function, zinc solubilizing function, phosphate solubilizing function, antibody-producing function, nitrogen fixing function, function of improving a plant’s nutrient acquisition, production of plant growth hormones, or any combination thereof, of one or more target microbes. Furthermore, the microbial signalers disclosed herein are able to rescue the reduction in the plant growthpromoting function (e.g., reduction in pathogen suppression and / or reduction in antibody production) exhibited by the target microbes in low nutrient soils. Therefore, the compositions disclosed herein (comprising at least one of the microbial signalers disclosed herein; and one or more target microbes disclosed herein) have unexpectedly superior plant growth-promotion functions (e.g. remarkably enhanced plant pathogen-inhibiting function, remarkably enhanced zinc solubilizing function, remarkably enhanced phosphate solubilizing function, remarkably enhanced antibody-producing function, remarkably enhanced nitrogen fixing function, remarkably enhanced function of improving a plant’s nutrient acquisition, remarkably enhanced production of plant growth hormones, or any combination thereof), as compared to the one or more target microbes disclosed herein alone, in standard soils as well as in low nutrient soils.

[0094] The disclosure provides compositions, comprising at least one microbial signaler, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of a target microbe. The disclosure further provides compositions, comprising: (a) at least one microbial signaler, and (b) at least one target microbe, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of the at least one target microbe.

[0095] In some embodiments, the compositions disclosed herein are microbial consortia.

[0096] In some embodiments, the compositions disclosed herein comprise one or more microbial signalers disclosed herein, and one or more target microbes disclosed herein. For instance, in some embodiments, the compositions disclosed herein comprise one or more microbial signalers listed in Table 1, and one or more target microbes listed in Table A.

[0097] In some embodiments, the compositions disclosed herein (that is, compositions comprising one or more microbial signalers disclosed herein, and one or more target microbes disclosed herein) have an enhanced ability to inhibit one or more plant pathogens, as compared to the one or more target microbes present in the compositions. In some embodiments, compositions comprising one or more microbial signalers listed in Table 1 and one or more target microbes listed in Table A have an enhanced ability to inhibit one or more plant pathogens listed in Table B, as compared to the one or more target microbes alone. In some embodiments, compositions comprising one or more microbial signalers listed in Table 1 and one or more target microbes listed in Table C have an enhanced ability to inhibit one or more plant pathogens listed in Table B, as compared to the one or more target microbes alone.Target Microbes

[0098] In some embodiments, the at least one target microbe belongs to the genus Talaromyces, Streptomyces, Bacillus, Trichoderma, Pseudomonas, Comamonas, or Enter obacter . In some embodiments, the at least one target microbe is Talaromyces flavus, Streptomyces griseoviridis, Streptomyces lydicus, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus amyloliquefaciens, Trichoderma harzianum, Pseudomonas putida, Pseudomonas chlororaphis, Comamonas testosterone, Citrobacter freundii, Enterobacter cloacae, or any combination thereof. In some embodiments, the at least one target microbe is Talaromyces flavus SAY-Y-94-01. In some embodiments, the at least one target microbe is Streptomyces lydicus WYEC 108.

[0099] In some embodiments, the target microbe is one or more of Talaromyces flavus, Trichoderma harzianum, Bacillus amyloliquefaciens, Streptomyces sp., Bacillus subtilis, Bacillus amyloliquefaciens, Streptomyces lydicus, Pseudomonas chlororaphis, Bacillus subtilis, Azospirillum brasilense, Trichoderma asperellum, Trichoderma gamsii, Pseudomonas putida, Comamonas testosterone, Citrobacter freundii, Enterobacter cloacae, Streptomyces spp., Trichoderma viride, Bacillus megaterium, Azospirillum spp., Bradyrhizobium japonicum, Rhizobium leguminosarum biovar viciae, Bradyrhizobium spp., Rhizobium leguminosarum, Azospirillum amazonense, Azospirillum lipoferum, Glomus intraradices, Rhizophagus intraradices, Glomus mosseaem, or any combination thereof.

[0100] In some embodiments, the target microbe belongs to a genus listed in Table A.Table A: Exemplary Genera of Target Microbes Present in the Compositions and Used in the Methods Disclosed Herein.

[0101] In some embodiments, the target microbe is present in a commercial product. Non-limiting examples of target microbes and the corresponding commercial products that may be present in the compositions disclosed herein, and / or used in the methods disclosed herein are listed in Table C below. Table C also lists the plant-growth promoting function of these exemplary target microbes. As described herein, the disclosed microbial signalers are capable of enhancing any one or more of the plant growth promoting functions of one or more of the target microbes listed in Table C.Table C: Exemplary Target Microbes Contained in Exemplary Commercial Products and Their Plant-growth Promoting Functions

[0102] N / A: Not availableln some embodiments, the target microbes have a genus ofBacillus and or Bradyrhizobium. In some embodiments, the target microbes have a species of one or more of Bacillus sublilis. Bacillus amyloliquefaciens, and Bradyrhizobium japonicum. In some embodiments, the target microbes have a species of one or more of Bacillus subtilis strain BUI 814, Bacillus amyloliquefaciens strain MB 1600, Bradyrhizobium japonicumPlant-Growth Promoting Functions

[0103] In some embodiments, the plant growth-promoting function comprises: (a) plant pathogen-inhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, (e) nitrogen fixing function, (f) a function of improving a plant’s nutrient acquisition, (f) production of plant growth hormones, (g) a nematicide function; or (h) any combination thereof.

[0104] In some embodiments, the at least one microbial signaler is capable of enhancing the plant pathogen-inhibiting function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the at least one microbialsignaler is capable of enhancing the plant pathogen-inhibiting function of the target microbe by at least about 5%.

[0105] In some embodiments, the at least one microbial signaler is capable of enhancing the plant pathogenic disease suppression function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the at least one microbial signaler is capable of enhancing the plant pathogen-inhibiting function of the target microbe by at least about 5%.

[0106] The enhancement of plant pathogenic disease suppression function as disclosed herein may be seen in any plant. Non-limiting examples of plants that may be used with the microbial signalers and / or compositions disclosed herein, or used in the methods disclosed herein are com, soybean, wheat, alfalfa, cotton, rice, oats, barley, oil crops (e.g. sunflower, canola), vegetable crops (e.g. potato, sweet potato, corn, cassava, yams, plantains, tomato, beans, sugar beets), sugar cane, fruit crops, grain crops, pulses, legumes, maize, millet, sorghum, tuber crops, feed crops, ornamental crops, industrial crops, food crops, fiber crops, beverage plants (e.g. tea, coffee), seed trees, nut trees, herb plants, bioenergy crops (e.g. switch grass, aspen), forest trees, and spices plants (e.g. saffron).

[0107] The plant pathogen is not limited, and may be a soil-borne plant pathogen, a seed-borne plant pathogen, a pathogen of leaves and / or foliage, a pathogen of fruit, a pathogen of stem, a pathogen of root, or any combination thereof. Non-limiting examples of the plant pathogen include plant pathogens listed below in Table B.Table B: Exemplary Plant Pathogens Inhibited by the disclosed signaling microbes and / or disclosed compositions, comprising the disclosed signaling microbes and the disclosed target microbes.

[0108] In some embodiments, the at least one microbial signaler is capable of enhancing the zinc solubilizing function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values andsubranges that lie therebetween). In some embodiments, the at least one microbial signaler is capable of enhancing the zinc solubilizing function of the target microbe by at least about 5%.

[0109] In some embodiments, the at least one microbial signaler is capable of enhancing the phosphate solubilizing function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the at least one microbial signaler is capable of enhancing the phosphate solubilizing function of the target microbe by at least about 5%. In some embodiments, the at least one microbial signaler is capable of enhancing the phosphate solubilizing function of the at least one target microbe under low nutrient conditions.

[0110] In some embodiments, the at least one microbial signaler is capable of enhancing the nitrogen fixing function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the at least one microbial signaler is capable of enhancing the nitrogen fixing function of the target microbe by at least about 5%. In some embodiments, the at least one microbial signaler is capable of enhancing the nitrogen fixing of the at least one target microbe under low nutrient conditions.Microbial Signalers

[0111] In some embodiments, the microbial signalers comprise polynucleotide sequences that share at least 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 95.1%, 95.2%, 95.3%, 95.4%, 95.5%, 95.6%, 95.7%,95.8%, 95.9%, 96%, 96.1%, 96.2%, 96.3%, 96.4%, 96.5%, 96.6%, 96.7%, 96.8%, 96.9%, 97%, 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or 100% sequence identity with the 16S Rma sequence, 18S Rma sequence, 23 S Rrna sequence, the internal transcribed spacer (ITS 1) sequence and / orITS2 sequence of any one of the microbial signalers listed in this specification.

[0112] The microbes disclosed herein may be matched to their nearest taxonomic groups by utilizing classification tools of the Ribosomal Database Project (RDP) for 16s Rrna sequences and the User-friendly Nordic ITS Ectomycorrhiza (UNITE) database for ITS Rrna sequences. Examples of matching microbes to their nearest taxa may be found in Lan et al.(2012. PLOS one. 7(3):e32491), Schloss and Westcott (2011. Appl. Environ. Microbiol. 77(10):3219-3226), and Koljalg et al. (2005. New Phytologist. 166(3): 1063-1068).

[0113] In some embodiments, the at least one microbial signaler is any microbe listed in Table 1 or Table D. A sample deposit of each of the microbial signalers disclosed in this application, including those set forth below in Table 1 or Table D, are maintained by Applicant at their internal laboratory facilities in St. Paul, Minnesota. In addition, a deposit of any one of the microbial signalers disclosed herein, including those set forth below in Table 1 or Table D, may be made in the future with an International Depositary Authority, as established under the Budapest Treaty and according to 37 CFR § 1.803(a)(1).Table 1: Exemplary Microbial SignalersName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolate>Name of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateCCAGATAATAATACCGCCTGCATGGCCGGGGGGTGAAAGCCCCGGCGGTGAAAGATGAGCCCGCGGCCTATCAGCTTGTTGGGGGGGGAAAGGCCCACCAAGGCGACGACGGGTAGCCCGCCTGAAAAGGCCAACGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAStreptomyces verneACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGC Streptomyces vinaceusGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTA Streptomyces virginiaeACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCG xanthophaeusAGGCTTAACCTCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACC GGTGGCGAAGGCGGATCTCTGGGCCATTACTGACG CTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTA GATACCCTGGTAGTCCACGCCGTAAACGTTGGGAA CTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGC AGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACG GCCGCAAGGCTAAAACCAAAAGAAATGAACGGGG GCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCG ACGCAACGCGAAGAACCTTACCAAGGCTTGACATA TACCGGAAAGCATTAGAGATAGGGCCCCCCTTGTG GTCGGTATACAGGGGGTGCATGGCTGTCGTCAGCTC GTGTCCGGAGAGATGTTGGGTTAAGTCCCGCAACG AGCGCAACCCCTGGCCTGTGTTGCCAGCATGCCCTT CGGGGGGATGGGGACTCACAGGAAACCGCCGGGGC AAACCCGGAGGAAGGTGGGGACGACGTCAAGTCAT CATGCCCCTTATGTCTTGGGCTGCACACGTGCTACA ATGGCCGGTACAATGAGCTGCGATACCGTGAGGTG GAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATT GGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATGCTGCGGTATCCGCCGGGGCGACTCATGACAAATGCAAAAAGAAAGATGAACCCCCTTCGCGAGGGGATTAGTGGCGAAACGGTGAGTAACACGTGCACAACCTGCACTTCACTCTGGGACAAAACCCGGAAACCGAGTCTAATACCGGATACGAACACACACCGCAGCATCGCGGGGTGGAAAGCTCCGGCGGTGAAGAAGAAGCCCGCGGCCTATAACAGCGGTGGTGGGGTAATGGCCCACCAAGGCGGACGACGGGTAGCCGGCCCGAAAAGGCCAACGGCCACACTGGGACTGAGAAAACGCCCAAAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCCCGGGTCTGCATTCGATACGGGCAAGCTAGAGTGCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCAATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAAGCCTTGACATACACCGGName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAAAACACTAGAGACAGGGCCCCCCTTGTGGGCCGT ATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCG TGAGAATGTTGGGTTAAGTCCCGCAACGAGCGCAA CCCTTGTCCTGTGTTGCCAGCATGCCCTTCGGGGTG ATGGGGACTCACAGGAGACCGCCGGGGCAAACTCC GAAGAAGGTGGGGACAACGTCAAGTCATCATGCCC CTTAAGTCCTGGGCTGCACAAGGCCAAAAAGCGCC ACAACAATGACATGCGATACCGCGAGGTGGAGCGA ATCTCAAAAAGCCGCTCTCAGTCCGAATGGAAGAC AGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTA ATCGCAGATCAGCATGCTGCGTTAATGCTC 1 AGGGGAGGTGCTTACACACGCACTCGAACCATGAAGACATCAGGGGTGAAAGAGTAGCCAACGGGGAAATAACACACGGGCAAACTGCACTGCACTCGGAGACAAACCCTGGAAACCGGGTCAAACAACAGACACCACGCATGCCTGCATGGGCGGGGGTGGAAAGCAACGCCGCGGAAAGATGAGCCCGCGCCATACAACCGTGTGGGTGGGGTAATGCCCCACAAAAGCGACGACGGCGAGACGGCCGCAGAGAGCGACACGCAAAACGGGAACGAAAACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCCCGGGTCTGCATTCGATACGGGCAAGCTAGAGTGCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAG GCGGATCTCTGGGCCAATACTGACGCTGAGGAGCG AAAGCGTGGGGAGCGAACAGGATTAGATACCCTGG TAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGG GCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCA TTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGA AGAACCTTACCAAGGCTTGACATACACCGGAAAAC ACTAGAGACAGGGCCCCCCTTGGGGTCGGTATACA GGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGA TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGT CCTGTGTGCCAACAAGCCCTTCCGGGGGAAGGGAC ACACACAAAAGACGGCACGCGAAAACGCGGAGGA AGGTGGGGACGACGGCAAGTCATCATGCCCCTTAT GTCTGGCGCTGCACACGTGCTACAAGCGCACATACAATGAACTGCGATACCGGCGAGGTGGAGACAATCT CAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCA ACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTAATGCCCT22 TCGGGCCCCTAAACACACAGGCAAGTCCAACGATGAAGCCTTTCGGGACCGATGAGCCGCGAAAGGGAGAGGAAAACGTGAGCAACCTGCCCATCACTCTGGCACAAGACCAGCAAACGACGGCTAAAACACGATACGACAAACGACCGCATGGTCTGGGTGTGGAAAGCTCCGGCGGTGAAAGAAGACCCCGCGACCTATCAGCTTGGTGGTGGGGTGATGGCATACCAAAGCGACGACGGGGAACCCGCCTGAGAGGGCGACCGCCAACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGAC>Name of 16S 16s rRNA sequence microbial rRNA signaler SEQ ID isolate NO:CTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTAATGCTACGGGAGGTGCCTACAAAACCACTCGAAAGACAAACCTACGACTCCGCAGAGGAATAGGGGCGAACGGGTGAGTAACACCGGGGAAAACTGCACTGCACTCAGGGACAAGCCCTGGAAACGGGCTCTAAAACCAGAAACGACATACGACCGCAACGCATGGTCGTGGAGGAAGCTCCGCCGGTGAAGGAAGAGCCCCCGCCCTATCAGCTGGTTGGTGGGGTGATGACCCAACAAGGCGACGACGGGTAGCCGGCCGAAAAGGGCGACCGGCCACACTGGGACTGAGAAAACGCCCAAAACCCTACGGGAGGCAGCAGTGGGGAATATGCAAAAATGGGCCAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTCCGGGTTGGAAAACCCCTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCCTGGAGACAGGGTCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTAAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTGCCAAGCATGCCCTTCGGGGTGATGGACACTCACAGGAGACTGCCGGGGCAAACGAGAAGGAAGGACGGGACGAACGCAAGTCATCATGCCCCTAATCTCGTGGCCTGCACACCGGCTACAATGACCCGTACAATGAACTGCGAAAACCCCAAGTGGAGCAAATCTCAAAAAGCCAGACCCAATTCGGACTGCACTCGGCAACTCGAACCCATGAACCCGGAATTGCTAATAAACACACAACACAATGCTGCGTAATGCCGTCCGGGCGGGTGCTTAAACATGCACGCAAACGATGAACCTCCCTCGTCGGAGGGGAATAGTGGCAAACGGGTGAGTAACACGTGGACAACCTGCCATTCACTACGCGACAAGCCCTGGAAACGGACACCAAAACCGGATAAGACCACCGACCCGAAGCATCTCGTGGTGGAAAGCTCCGCCGGTGAAAGATGAGCCCGCCGCCAATCAGCTTStreptomyces libaniGGGAACCCGCCTGAGAGGGCGACCGGCCACACTGG Streptomyces lydicusGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA StreptomycesTGCAGCGACGCCGCGTGAGGGATGACCGCCCTTCG nigrescensGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAGA Streptomyces platensisGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAG Streptomyces rimosusCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAG tubercidicusAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGA>Name of 16S 16s rRNA sequence microbial rRNA signaler SEQ ID isolate NO:GGAGCGAAAGCGTGGGGAGCGAACAGGATTAGAT ACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTA GGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGC TAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCC GCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCC GCACAAGCAGCGGAGCATGTGGCTTAATTCGACGC AACGCGAAGAACCTTACCAAGGCTTGACATACACC GGAAAACCCTGGAGACAGGGTCCCCCTTGTGGTCG GTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGT CGTGAAGATGTTGGGTTAAGTCCCGCAACGAGCGC AACCCTGGTCCGGGGTGGCCAGCATGCCCTTCGGG GTGATGGGGACTCACAGAAAACGGCCGGGGTCAAC TCGGAGGAAGGTGGGGACGACGTCAAGTCATCATG CCCCTTATGTCTTGGGCTGCACACGTGCTACAATGG CCGGTACAATGAGCTGCGATACCGCGAGGTGGAGC GAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGG TCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTATGCGGCTGATTCGAAAGGCCGCGGCTAACACATGCAGGCGAACGATGAACCTCCTTCGGGAGGGGATTAGTGGCGAACGGGTGAGTAACACCTCGACAATATGCCAATCACGCAGGAACAAGCCATGGAAACAGGGACAAATAAACGGTATATCAAGACCACCCAACGCATGGTCTGCTGGGGGAAAGCTCCCGCGGCGAAAGATGAGCCCCCGCCCTATCAGCTGGGTGGGGGGATGATGGCCTACAAAAGCGACGACGGGTAGCCGACCGCAGAGAGCGACACGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATGCAAAAAGGGGCCAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCCTGGAGAACAGGGTCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAAATGTTGGGATAAAGCCCCGCAACGAGCGCAAACCCTTGTTCTGTGTTGCCAGCATGCCCCTCCGGGGGAAGGGGACTCACAGAAAACTGCCGGGGCCAACTCGAAGAAAGGGGACGACGAAGTCAAGTCATCAGGCCCCTAAGGTCTTGGGCTGCAAACGTGCCAAAATGGCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTAATGCGCAGGGGCGGGGTGCTTACACATGCAAGTCGAACGAT Streptomyces auratusGAACCCCGGCGGGCTGGAACACTGGCAAACGAACGAGGAACACCTCGACAATATGCCACCGTCACACCGGGACAAGCCCTGCAAACACAGCCTAAAACCGGATAAName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the i l SE ID i bi l i l>Name of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAACCCTGGAGACAGGGTCCCCCTTGTGGTCGGTGTA CAGGTGGTGCATGGCTGTCGTCACCTCGTGTCGTGA GATGTTGGGTTAAGTCCCGCAACAACCGCAACCCTT GTTCCGGGGTGCCAGCATGCCCTTCGGGGTGATGG GCACTCACAAGAAACGGCCCGGGTCAACCCCGAGG AAGGTGGGGACGACGTCAAGTCATCATGCCCCTTA TGTCTTGGGCTGCACACGTGCTACAATGGCCGGTAC AATGAGCTGCGATACCGCGAGGTGGAGCGAATCTC AAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCA GATCAGCATGCTGCGTATGCACCCAGCCGGTGCTTAAACACACAGTACAAGTCGACA Streptomyces avidiniiAAAGATAAAGCCCTTCGGAGGGGATTAGTGGCAAACGGGTGAGTAACACGTGAGCAACCTGCCATTCACCCTCAGACAAACAAGCGGAAACGGAGTCTAATACAC colombiensisGATACCACTCCTCCACGCATGGTCCGGGGGTGGAAStreptomyces flaveusAGCTCCGGCGGTGAAGAAGGAGCCCGCCGCCTATCAGCTGGTGGGGGGGGTAAGAGCCCACCAAGGCGAC StreptomycesGACCGGGAACCCGCCTGAGAGGGCGACCGGCCACA goshikiensis CTGGGACTGAGACACGGCCCAGACTCCTACGGGAG GCAGCAGTGGGGAATATTGCACAATGGGCGAAAGC Streptomyces CTGATGCAGCGACGCCGCGTGAGGGATGACGGCCT lavendulaeTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCG Streptomyces AAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAA spororaveus CTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGC AAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTC Streptomyces subrutilus GTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCCCGGGTCTGCATTCGATACGGGCAAG CTAGAGTGCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCAATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGA CGCAACGCGAAGAACCTTACCAAGGCTTGACATAC ACCGGAAAACACTAGAGACAGGGCCCCCCTTGTGG TCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCG TGTCGTGAGATGTTGGGTTAAGTCCCCAAACGAGC GCAACCCTTGTCCTGTGTTGCCAGCATGCCCTTCGG GGTGATGGGAACCCACAAGAAACCGCCGGGGTCAA CTCGAAGAAAGGGGGGGAACAACTCAAGTCATCAT GCCCCTAATCTCTTGGGCTGCACACGTGCTACAATG GCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGG GTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGGTAATGCTCCGGTGCCGACGAACACAGGCAAGGCGAAAGAACAACCAACTGCGAGAAGGGATAAGCCGCGAACGAATAAGGAAAACGTGAGCAACCTGCCCATCACTCCAGCACAAACCCTGGAAACGGGGTCTAATACACGATACGACCTCCGACCGCAAGGTCTGGGGGTGGAAAGCTCCGCCGGAGAAGGAAGAACCCGCGGCCTATCAGCTGGGTGGGGGGGTGATGCCCTACAAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCACCCAAAATGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATGGCAAAAAGGGCCAAAACCTGATGC StreptomycesAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTT nigrescensGGAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGA Streptomyces platensisName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateCGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTG Streptomyces r mosus CCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTT:GTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCG Streptomyces GCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAA tubercidicus CCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTT CGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTG AAATGCGCAGATATCAGGAGGAACACCGGTGGCGA AGGCGGATCTCTGGGCCGATACTGACGCTGAGGAG CGAAAGCGTGGGGAGCGAACAGGATTAGATACCCT GGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGT GGGCGACATTCCACGTCGTCCGTGCCGCAGCTAAC GCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAA GGCTAAAACTCAAAGGAATTGACGGGGGCCCGCAC AAGCAGCGGAGCATGTGGCTTAATTCGACGCAACG CGAAGAACCTTACCAAGGCTTGACATACACCGGAA AACCCTGGAGACAGGGTCCCCCTTGTGGTCGGTGTA CAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGA GATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTT GTTCTGTGTTGCCAGCATGCCCTTCGGGGTGATGGG GACCCAAAAGAGACTGCCGGGGCCAACTCGGAGAA AGGGGACCACGACAGCAAGTCATCATGCCCCTAAT GTCTGGCGCCAAACACGTGCTACAAGGGACCGGAC AATGAACTGCCATACCGCGAAGGTGGAACGAATCT CAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCA ACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGC AGATCAGCATGCTGCGGTATTGCTC9 AAGGCGCGCGAACACAAATGCAAGTCCAAAAAGG Streptomyces AAACCCTTCGAGGTGGAATAGGCGAGAACGGGTGA pinnamonensis GTAACACGGGGGCAACCGGCACTGCACTCACGGAC AAGCCCAAGAAAAACGGTCTAATACACGATAATAC Streptomyces cirratus TCCTGCCGCAATGGGCCGGGGTTGAAAGCTCCGGC Streptomyces GCGGAAAGATGAGCCCGCGGCCTATCAGCTGGTTG GTGGGGTAATGGCCCACAAAGGCGACGACGGGTAG goshikiensis CCAGCCGCAGAGAGCGACACGCCACACTGGGACTG Streptomyces AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTG lavendulcieGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG Streptomyces senoensis CGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGT AAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACG Streptomyces GTACCTGCAGAAGAAGCGCCGGGCTAACTACGTGC sporoverrucosus CAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG TCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGG Streptomyces vinaceus CTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACC Streptomyces virginiae TCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAG GCGGATCTCTGGGCCATTACTGACGCTGAGGAGCG AAAGCGTGGGGAGCGAACAGGATTAGATACCCTGG TAGTCCACGCCGTAAACGTTGGGAACTAGGTGTTG GCGACATTCCACGTCGTCGGTGCCGCAGCTAACGC ATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGG CTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCG AAGAACCTTACCAAGGCTTGACATATACCGGAAAG CATTAGAGATAGTGCCCCCCTTGTGGTCGGTATACA GGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGA TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTGG CCCGGTGTTGCCAGCATGCCCTTCGGGGGAAGGGA CACCAAAAGAAAACCGCCGCGGTCAACTCGAAGAAAGGTGGGGACGACGTCAAGTCATCAAGCCCCTTAT GGCTCGGGCTGCACACGTGCTACAATGCCCGGTACAATGAACTGCCATACCGAGAAGTGAAGCGAATCTCName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAAAAAGCCGGCCCCACATCCGATTGGGGGCGGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATGCTGCGGTTATGCTAAGGGCGGCGTGCTGACACATAAAACTCAAACGATGAAGCAAGTCCGAATCAATGAATGCCGAAAGGGAAGAGGAACACGTGAGCAACCGGCCAATCACTCCGCGACAAGCCCTGGAAACGGGGTCTAATACACGATACCAATCCTGCCTGCATGACCGGGGGGTGAAAGCTAAGGCGGCGAAAAAAGAACCCGCGGCCAATCAGCTTGGCGGTGGGGGAAAAGCCCACCAAGGCGACGACGGGTAGCCGCCCTGAAAAGGCCAACGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCTCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTAACCAAGGCTTGACATATACCGGAAAGCATTAGAGATAGTGCCCCCCTGGGGGTCGCGAAAAAAGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTAAAGCCCCCAAAAGAGCACAACCCTTGTCCTCTGTGGCCAGCATGCCCTTCGGGGGAAGGAGGACTCACAGGAAACCCCCGGGGTCAACTCGGAGAAAGGTGGGGACGACCTCAAGTCATCATGCCCCAGACGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGTGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGC AGATCAGCATGCTGCGTAATGGCCAATCATGGCGCCGGGCTAACACATGCAGTCCAACG Streptomyces echinatus AAGAAGCCCGGCGGGGTGGATTAGAGGCGAAACG GTGAGTAACACGTGAGCAACCTGCACCGCACTCTG Streptomyces GGACAAGCCCTGGAAAAGGGGTCCAAGACCGGATA filipinensis TAAGACTGAGAGGCAGCATCCCCGGGTGTAAAGCT CCGCCGGTGCAAGATGAGCCCCCCGCCTATCAGCT Streptomyces AGCTGGTGAGAGAAAGGCTCACCAAGGCGACGACG gulbargensis GGTAGCCCGCCTGAGAGGGCGACCGGCCACACTGG Streptomyces GACTGAGACACGGCCCAGACTCCTACGGGAGGCAG longwoodensis CAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA TGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGG Streptomyces GTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAGAG myxogenesTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTAC Streptomyces GTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGC novaecaesareae GTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAG GCGGCTTGTCACGTCGATTGTGAAAGCCCGAGGCTT Streptomyces AACCTCGGGTCTGCAGTCGATACGGGCTAGCTAGA spectcibilis GTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCG StreptomycesGTGAAATGCGCAGATATCAGGAGGAACACCGGTGG tanashiensisCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATAC CCTGGTAGTCCACGCCGTAAACGGTGGGAACTAGG TGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTA ACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGC AAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGC ACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAA CGCGAAGAACCTTACCAAGGCTTGACATACACCGG AAAGCATTAGAGATAGTGCCCCCCTTGTGGTCGGTG TAACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCG GGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAC CCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGGAA TGGGAACCCACAGAAAACCCCCGGGCAAAACTCGA AGAAAGGGACGGACGACCTCAAGTCATCATGCCCCTCATATCTCGGGCTGCAAACGTGCTACAATGGCCGG TACAATGAACTGCAAAACCGAGAGGTAGAGCGAAT CTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGC AACTCGACCCCATGAAGTCGGAGTCGCTAGTAATC GCAGATCAGCATGCTGCGTATGCGACAGCGCCGCGTGCGTACACAAACAGTCCAACGACGAACACCATCGGGAAGCATCAGTGGAGAACGGGTGAGGAAAACGTGAGCAACCGGCACTGCACCATGGGACAAGCCCTGGAAACGCGGACGAATACAGAAGATGAACCTGGGAGGCATCTCCCGGCGGGTAAAACTCCGGCGGTGCAGCAGAAGACCCCCGCCTATCAACCTGTGGGGGAGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCGAAAAGGGCCAACGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGG GGAATATTGCACAATGGGCGAAAACCTGATGCAGC GACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTA AACCTCTTTCAGCAGGGAAGAAGCGAGAGTGACGG TACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTC CGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCT TGTCACGTCGATTGTGAAAGCCCGAGGCTTAACCTC GGGTCTGCAGTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGG CGGATCTCTGGGCCATTACTGACGCTGAGGAGCGA AAGCGTGGGGAGCGAACAGGATTAGATACCCTGGT AGTCCACGCCGTAAACGGTGGGAACTAGGTGTTGG CGACATTCCACGTCGTCGGTGCCGCAGCTAACGCAT TAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCT AAAACTCAAAGGAATTGACGGGGGCCCGCACAAGC GGCGGAGCATGTGGCTTAATTCGACGCAACGCGAA GAACCTTACCAAGGCTTGACATACACCGGAAAGCA TTAGAGATAGTGCCCCCCTTGTGGTCGGTGTACAGG TGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATG TTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCC GGGGTTGCCAGCATGCCCTCCGGGGTGATGGGGAC TCAAAAGAAACCGCCGGGGTCAACTCGGAGAAAGG TGGGAACAACGTCAAGTCATCATGCCCCTTATGTCT TGGGCCGGCAACACGTGCTACAATGGCCGGTACAA TGAGCTGCGATACCGTGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTC GACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATGCTGCGGTAATGCGCAGGGGGGGCGAGCATACACACGAAGTCCAACCACGAACATCCTTCGCGAAACGATGAGCGCCGAAAAGATGAGCAACACGTGGGCAAGATAACCTGCACACTGCCACAAGCACAGCAAAAACGGACTAATACCGGATAAC>Name of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAAATCAAACCGCATGCACGGCGGGGGGAAAGCTCC StreptomycesGGCGGTGAAAGATGAGCCCGCGGCCTATCAGCTGG lavendulaeGTGGGGAGGCCACGGCCTACCAAGGCGAAAACGACGACCCGACCGCAGAGAGCGACACGCCACACTGGGA StreptomycesCTGAGACACGGCCCAGACTCCTACGGGAGGCAGCA sioyaensisGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGStreptomycesCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGT spororaveusTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGT Streptomyces verneGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGT Streptomyces virginiaeTGTCCGGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTT SAACCCCGGGTCTGCATTCGATACGGGCAAGCTAGAGTGCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGG CGAAGGCGGATCTCTGGGCCAATACTGACGCTGAG GAGCGAAAGCGTGGGGAGCGAACAGGATTAGATAC CCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGG TGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTA ACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGC AAGGCTAAAACTCAAAGAAATGAACGGGGGCCCGC ACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAA ACCCAAAAAACCTAACAAAGCTTGACATACACCGG AAAACACTAAAAACAGGGCCCCCCTTGTGGTCGGT ATACAGGGGGGCAAGGCCTGTCGTCAGCTCGTGTC GTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA CCCTTGTCCTGTGTTGCCAGCATGCCCTTCGGGGTG ATGGGGACTCACAGGAGACCGCCGGGGTCAACTCG GAGGAAGGTGGGGACGACGTCAAGTCATCATGCCC CTTATGTCTTGGGCTGCACACGTGCTACAATGGCCG GTACAATGAGCTGCGATACCGTGAGGTGGAGCGAA TCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAAT CGCAGATCAGCATGCTGCGGTAATGGGCCGGGCGCGTGCTTACACATGCAAGTCGAACCAAAAACCACCGTCAAGAGGCGATAAGTGGCGAACGGGTGACAAACACGGAGGCAATATGCCCCACACTCAGGAACAAGCCATGGAAAACGGGACAAATAACGGGATACAACATACGACCGCAACGCATGGTCGTGGAAAACTCCGGCCGCGAAAGATGAGCCCGCGCCATATCAGCTTGCAGCTGGGGTGAGGGCATACCAAAGCGACGACGGGTAGCCGGCCGCAGAGAGCGACACCCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCCGGGTGGAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGA>Name of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAAACCCTGGAGACAGGGTCCCCCTTGTGGTCGGTGT ACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTG AGAAGGTGGGTTAAGTCCCGCAACGAGCGCAACCC TTGTTCTGTGTTGCAACCAAGCCCTTCGGGGTGATG GGGACTCACAGGAGACTGCCGGGGTCAACCCGGAAGAAAGTGGGGACGACGTCAAGTCATCATGCCCCTT ATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTA CAATGAGCTGCGATACCGCGAGGTGGAGCGAATCT CAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCA ACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTAATGCACACGCGGCGGTGCTTAAACACGCAAGCGAACGATGAAGCCCTTCGGGGTGGATTAGAGAAGAACGGGTGAGTAACACACGGGCAACCTGCCATTCACTCCGGCACAAACCCTGGAAACAGGGTCTAATAACCGATACCACTACAACCTGCAGCAGCCGGGGTTGAAAGCTCCCGCGGTGAAGAAAGAACCCGCGGCCTATCAGCTGGTTGGTGGGGGAAAGGCCCAACAAAGCGACGACGGGTAGCCGCCCAGAGAAGGCCAACGGCAAAACGGGAACGGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCTCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCG AAGAACCTTACCAAGGCTTGACATATACCGGAAAG CATTAGAGATAGTGCCCCCCTTGTGGTCGGTATACA GGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAAA GGTGGGGTAAAGTCCCGCAACAAGCGCAACCCTTG TCCTGTGTTGCCAGCATGCCCTTCGGGGTGATGGGG ACTCACAGGAGACCGCCGGGGTCAACTCGGAGGAA GGTGGGGACGACGTCAAGTCATCATGCCCCTTATGT CTTGGGCTGCACACGTGCTACAATGGCCGGTACAAT GAGCTGCGATACCGTGAGGTGGAGCGAATCTCAAA AAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCG ACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGAT CAGCATGCTGCGGTAATGCGTCCGGGCGCGCGAAAAAACATGCAAGTCGAACAATGAACCCCCTTCGGAAGGAGATTAGTGGCGAACGGGTGAGGAAAACGTGGGCAATCTGCCCTTCACTAACGGACAAGCCCCGGAAACCGGACCGAATAACCGATACGACCACACAACGCATGGTCTGGTGGGGGAAAGCTCCGCCGGTGAAAGATGAGCCCGCGCCCTATCAGCTGGTGGGTGAGGTGATGGCCTACCAAAGCGACGACGGGTAGCAGCCCGCAGAGAGCGACACGCCACACTGGGACTGAGACACGGCCCAGACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTT StreptomycesGTAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGA:ossamyceticusCGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTT Streptomyces platensisGTCCGGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAACGTCTGGAGACAGGCGCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGGAAGGGGAACCCACAGGAGACTGCCGGGCACAACTCGGAGAAAGGGGGGGAACAACGCAAGTCATCATGCCCCTTATGTCTGGCGCCCAACACGTGCTACAATGGCCGGTACAAGGAACTGCAACACAGCGAAATGGAGCCAATCTCAAAAAGCCGGTCTCAATTCGAATTGGAGGCACCAACTCGACCCCAAGAAGTCGGAGTGGCTAATAATCCAATCACAGATCAGCACGCTGCGTATGCGC13 CGGGCGGGTGCTTACACATGCAGTCGAACAATGAACCACATCGGGGTAGATTAGTAACCAACGGATGAGATATGAGGAACACGTGAGCAAACTGCCATTCACACTGGGACAAGCCCTGGAAACGGAACCGAATACACGATAACACTCTGTCCCGCATCGCACGACCGGTAAAAGCTCCGGCGGTGAAAGATGAGACCCCGCCCTATCAGCTTGTGGGTGGGGTAATGGCCTACAAAAGCGACGACGGGTAGCCCGCCCGAAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAA GCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTAG AGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGC GGTGAAATGCGCAGATATCAGGAGGAACACCGGTG GCGAAGGCGGATCTCTGGGCCATTACTGACGCTGA GGAGCGAAAGCGTGGGGAGCGAACAGGATTAGAT ACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTA GGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGC TAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCC GCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCC GCACAAGCAGCGGAGCATGTGGCTTAATTCGACGC AACGCGAAGAACCTTACCAAGGCTTGACATATACC GGAAAGCATCAGAGATGGTGCCCCCCTTGTGGTCG GTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGT CGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA ACCCTTGTTCTGTGTTGCCAGCATGCCCTCCGGGGGAAGGGACACCAAAAGAAGACTGCCGGGGTCAACCCCGAAAAAAATGGGGACGACGTCAAGTCATCATGCCName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolate>Name of 16S 16s rRNA sequence microbial rRNA signaler SEQ ID isolate NO:CAGGGGGGTGCTTACACATGCAGTCGAACGATCAACAACTGCGCGCTGGATTAACAGCGAAAGGGTGAGTAAAACGTGGGCAATATGACCTTCACTCAGAGACAAGAACGGGAAAACCGGGCCAATACCGCATAACACCTGCAACGGCATCCGGATACGATAAAAGCTCCGGCATGCAGGTGAAGCTCCGCGAAAGATGAGCCCGCGCCA TAACAGCTGGTTGGTGAGGGAGGGGATCACAAAGGAGACGACGACGAGCAGCCCTGAGAGGGCGACCGCCAACACTGGGACTGAGACACGGCCCAGACTCCAACGGGAGGCAGCAGTGGGGAATATGCAACAAAGGGCCAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAG GACGG ACC GCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGA GTACGGCCGCAAGGCTAAAACTCAAAGGAATTGAC GGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAA TTCGACGCAACGCGAAGAACCTTACCAAGGCTTGA CATACACCGGAAACCACCAGAGATAGTCCCCCCCT GGGGGTCGGTATACAGGTGGTGCAAGGCTGTCGTC AGCTCGTGTCGTGAGATGTTGGGTAAAGCCCCCAA ACAACCCCAACCCTTGTTCCGGTGTTGCCAGCATGCCCTTCCGGGTGATGGGGACTCACAGGAGACCGCCG GGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAG TCATCATGCCCCTTATGTCTTGGGCTGCACACGTGC TACAATGGCCGGTACAATGAGCTGCGATACCGTGA GGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCG GATTGGGGTCTGCAACTCGACCCCATGAAGTCGGA GTCGCTAGTAATCGCAGATCAGCATGCTGCGTATCC GCGGGGGGCGTGCTTACACATGCAAGTCGAACGATAAAACACTCCGCAATGGAATACAGACGAACCCGTCAGTAACACGTGGAAAATCGCAACGTCACTAACGGACAAGCCCTGCAAACGGACAAGAAGACCGGTTTATACCAACACCGCCTGCATGGCCGGGGGTTGAAAGCTCCGGCGCGGAAAGATGAGCCCGCGCCCTATCAGCTGGTTGGTGGGGGAAAAGCCCACCAAGGCGACGACGGGTAGCCGCCCGCAGAGAGCGACACCCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCTCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGG>Name of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateCCCACTCGCAGTGGATTAGTGGCAAACGAGAGAGT colombiensisAACACGTGAACAAGCGCACCTTCACGCTAGGACAAGCCATGGAAACGGGACAGAAGACCGGACAACACG Streptomyces flaveusACAGCATCCAGCGGCAGGGCAGGAAAGAACCGCCG StreptomycesCGGAAAGATGAGCCCGCGGCCTATCAGCTTGGTGG lavendulae>Name of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID i bi l i l isolate NO:AGATGTTGGGTTAAGTCCCCCAAACAACCCAACCCT TGTTCTGTGTTGCCAGCATGCCCTTCCAGGTGATGG GGACTCACAGGAAAACGCCCGGGGTCAACCCCGAG GAAGGGGACGACGACGTCAAGTCATCATGCCCCTT ATGTCCTGGGCTGCACACGGGCTACAAGCGCACAT AAAAGCAGCTGCGAAACCGCCAAGGGGAACCAATC TCAAAAAGCCAGTCTCAGTCCGAATTGGGGTCTGCAACCCGAACACAAGAACTCAGAGGCGCTAGGAACC CCAGATCAGCATGCTGCTTAATGCGACCTGGCGCGTCCAAACACAATGCAAGTCCAACGAAG AAGCCCTTCGGGGTGGATTAGTGGCCAACGAGTGA GTAACCAACGGGGACAAACTGCCAATCACTCACGGACAAGACAAGCAAAACGGGTCTAATACCAGAAAAT ACACCTGCCAGCATCGGCGGGGGTGGAAAAAGCCG GCGGGCAAGAAGAAGACCCCCGCCTATCAGCTGGT GGGGGAGGTAATGGCCCACCAAAGCGACGACGGGT AGCCCGCCGAAAAGGGCGACCGCCAACACTGGGACTGAAAAAACGGCCCAGACTCCTACGGGAGGCAGCA Streptomyces mirabilis GTGGGGAATATTGCACAATGGGCGAAAGCCTGATG CAGCGACGCCGCGTGAGGGATGACGGCCTCCGGGTTGGAAAACCCCTTCAGCAGGGAAGAAGCGAAAGTG ACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGT GCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGT TGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGA GCGAAAGCGTGGGGAGCGAACAGGATTAGATACCC TGGTAGTCCACGCCGTAAACGGTGGGAACTAGGTG TTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAAC GCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAA GGCTAAAACTCAAAGGAATTGACGGGGGCCCGCAC AAGCAGCGGAGCATGTGGCTTAATTCGACGCAACG CGAAGAACCTTACCAAGGCTTGACATACACCGGAA ACCACCAGAGATAGTCCCCCCCTGGGGGCCGGAGA AAAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTG AGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCC TTGTTCTGTGTTGCCAGCATGCCCTTCGGGGTGATG GGGACTCACAGGAGACTGCCGGGGTCAACTCGGAG GAAGGTGGGGACGACGTCAAGTCATCATGCCCCTT ATGTCTTGGGCTGCACACGTGCTACAATGGCAGGTA CAATGAGCTGCGAAGCCGTGAGGCGGAGCGAATCTCAAAAAGCCTGTCTCAGTTCGGATTGGGGTCTGCAA CTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTATGCCTACGGGCGGGTGCTTACACATGCAGTCGAACGATGAAGCCCTTCGGGGTGGATTAAGAACAAACGGGCGAGTCTAACACCAAGGCAAGCTGCCCTACACTCGAAGACAAGCACGGGAAAACGGGCCAAAGACCGCATAACAAGCCTCACGCCTCCATGCGAGCGAGGCGAAACATCCCGCGGTGAAGGATCAGCGGGAGATGACCCGCGGCCTATCAGCTGGTTGGTGGGGGAAACGCCCACCAAGGCGACGACGGGAACCCGGCCCGAAAAGGCCAACGCCAACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGCCGAAAACCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCCGGGTGTAAACCTCTTTCAGCAGGGAAGName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAAGCGAAAGTGACGGTAACTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG xanthophaeusGGCGCAAGCGTTGTCCGGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGGCTTAACCTCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGA ACACCGGTGGCGAAGGCGGATCTCTGGGCCATTAC TGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACA GGATTAGATACCCTGGTAGTCCACGCCGTAAACGTT GGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGT GCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGA AGAACGCCCCAAAGCCAAAAACCAAAGGAATTGAC GGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAA TTCGACGCAACGCGAAGAACCTTACCAAGGCTTGA CATATACCGGAAAGCATTAGAGATAGTGCCCCCCTT GTGGTCGGTAAAAAAGGTGGTGCATGGCTGTCGTC AGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA CGAGCGCAACCCTTGTCCTGTGTTGCCAGCATGCCC TTCGGGGTGATGGGGACTCACAGGAGACCGCCGGG GTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTC ATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTA CAATGGCCGGTACAATGAGCTGCGATACCGTGAGG TGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGA TTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTC GCTAGTAATCGCAGATCAGCATGCTGCGTATGCGG GCGGGGGGCGTGAATACACATGCAATGTCGAACCATAAAACCCTTCGGGGAGGATTAGTGGCGAACGGGTAAGGAAAACGTGAGCAACCTGCACGCCACTCGGGGACAAGCCCTGGAAACGGACCAGAATAACGGATAACACCAGACACCGCAACCAGCTGAGAGAAAAGCTCCGGCGGTGAAGGATGAGCCCGCGGCCTATCAGCTGGTGGGTGAAGTAGTGGCTCACCAAGGCAACGACGACGAGCAGCCCCGAGAGAGCCAACGCCAACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGGTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGG CTAAAACTCAAAGAAATGAACGGGGGCCCGCACAA GCAGCGGAGCATGTGGCTTAATTCCAACCAACGCG AAGAACCTTACCAAGGCTTGACATACACCGGAAACGGCCAGAGATGGTCGCCCCCTGGGGGGCCGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGGAAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCAGGTACName of 16S 16s rRNA sequence Possible Taxonomic microbial rRNA Assignments for the signaler SEQ ID microbial signaler isolate NO: isolateAATGAGCTGCGAAGCCGTGAGGCGGAGCGAATCTCAAAAAGCCTGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGTAATTGCCGTGJBS5615 TCGTGGCGCGCGCGTGCTAACACATGAAAGTCGAACAAGGAACCTCCTTCGCGAGAAGACTAGTGGCAAACGGGTGAGTAACACCGGAGAAATCTGCCATTCACTACGGGACAAGCCCTGGAAAACGGGTCTAATACCGGATACCAATACACGAGGCAGCACCTGGAGGTGGAAAGCTCCGGCGGTGAAGAAGAAGCCCCCCGCCTATCAGCTGGGTGGTGAGGTAACGCCCCAACAAAGCGACGACGGGTAGCAGCCCCGAAAAGGCCAACGGCAACACTGGGACTGAGACACGGCCCAGACTCCAACGGAAGGCAGCAGTGGGGAATATTGCACAATGGCCGAAAGCCGAAGCAACCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGAGG CTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTA GAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAG;CGGTGAAATGCGCAGATATCAGGAGGAACACCGGT GGCGAAGGCGGATCTCTGGGCCATTACTGACGCTG AGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGA : TACCCTGGTAGTCCACGCCGTAAACGGTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAG i CTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAACCACTAGAGACAAGCGCCCCCTTGTGGTCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGGCCGGAAGAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGTGATGGGGACTCACAGGAGACCGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGTGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATGCTGCGGTAAATGCGACTable D: Exemplary Microbial Signalers and Examples of Enhanced Target Microbe Functions

[0114] In some embodiments, the at least one microbial signaler is Streptomyces avidinii, Streptomyces colombiensis, Streptomyces lavendulae, Streptomyces roseochromogenus, Streptomyces spororaveus, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces xanthophaeus, Streptomyces angustmyceticus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces platensis, Streptomyces rimosus, Streptomyces tubercidicus, Streptomyces bungoensis, Streptomyces cyslabdanicus, Streptomyces galbus, Streptomyces kagawaensis, Streptomyces lasaliensis, Streptomyces lasalocidi, Streptomyces longwoodensis, Streptomyces spinichromogenes, Streptomyces cirratus, Streptomyces nojiriensis, Streptomyces verne, Streptomyces vinaceus, Streptomyces virginiae, Streptomyces catenulae, Streptomyces cinereus, Streptomyces griseocarneus, Streptomyces sioyaensis, Streptomyces subrutilus, Streptomyces atrolaccus, Streptomyces auratus, Streptomyces fagopyri, Streptomyces kaempferi, Streptomyces mirabilis, Streptomyces olivochromogenes, Streptomyces chattanoogensis, Streptomyces flaveus, Streptomyces goshikiensis, Streptomyces cinnamonensis, Streptomyces senoensis, Streptomyces echinatus, Streptomyces fdipinensis, Streptomyces gulbargensis, Streptomyces myxogenes, Streptomyces novaecaesareae, Streptomyces spectabilis, Streptomyces tanashiensis, Streptomyces ginsengisoli, Streptomyces graminisoli, Streptomyces lucensis, Streptomyces yaanensis, Streptomyces caniferus, Streptomyces decoyicus, Streptomyces glebosus, Streptomyces ossamyceticus, Streptomyces badius, Streptomyces cyaneofuscatus, Streptomyces flavogriseus, Streptomyces griseus, Streptomyces mediolani, Streptomyces praecox, Streptomyces pratensis, Streptomyces omiyaensis, Streptomyces aquilus, Streptomyces caeruleatus, Streptomyces griseochromogenes, Streptomyces pseudovenezuelae, Streptomyces viridochromogenes, Streptomyces argenteolus, Streptomyces chrestomyceticus, Streptomyces coelicolor, Streptomyces microsporus, Streptomyces aureus, Streptomyces lutosisoli, Streptomyces minoensis, Streptomyces rhizosphaerihabitans, Streptomyces griseoruber, Streptomyces scabiei, Streptomyces achromogenes, Streptomyces canarius, Streptomyces capoamus, Streptomyces cellostaticus, or Streptomyces katrae. In some embodiments, the at least one microbial signaler is Streptomyces echinatus, Streptomyces galbus, Streptomyces lavendulae, Streptomyces libani, Streptomyces lydicus, Streptomyces mirabilis, or Streptomyces venezuelae .

[0115] In some embodiments, the at least one microbial signaler belongs to the genus Streptomyces, Fusarium, o Bacillus. In some embodiments, the at least one microbial signaler belongs to the genus Streptomyces .

[0116] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID Nos: 1-36. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to one or more of the following SEQ ID Nos: 1-36. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID Nos: 1-36.

[0117] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 1. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 1. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 1. In some embodiments, the at least one microbial signaler is Streptomyces echinatus, Streptomyces filipinensis, Streptomyces gulbargensis, Streptomyces longwoodensis, Streptomyces myxogenes, Streptomyces novaecaesareae, Streptomyces spectabilis, or Streptomyces tanashiensis .

[0118] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at leastabout 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 2. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 2. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 2. In some embodiments, the at least one microbial signaler is Streptomyces echinatus, Streptomyces ginsengisoli, Streptomyces graminisoli, Streptomyces gulbargensis, Streptomyces longwoodensis, Streptomyces lucensis, Streptomyces tanashiensis, or Streptomyces yaanensis.

[0119] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 3. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 3. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 3. In some embodiments, the at least one microbial signaler is Streptomyces bungoensis, Streptomyces cyslabdanicus, Streptomyces galbus, Streptomyces kagawaensis, Streptomyces lasaliensis, Streptomyces lasalocidi, Streptomyces longwoodensis, or Streptomyces spinichromogenes .

[0120] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 4. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 4. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 4. In some embodiments, the at least one microbial signaler is Streptomyces auratus, Streptomyces cyslabdanicus, Streptomyces fagopyri, Streptomyces galbus, Streptomyces kaempferi, Streptomyces mirabilis, or Streptomyces olivochromogenes .

[0121] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 5. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 5. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 5. In some embodiments, the at least one microbial signaler is Streptomyces achromogenes, Streptomyces bungoensis, Streptomyces canarius, Streptomyces capoamus, Streptomyces cellostaticus, Streptomyces galbus, Streptomyces katrae, or Streptomyces spinichromogenes .

[0122] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 6. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 6. In some embodiments, the at least one microbial signalercomprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 6. In some embodiments, the at least one microbial signaler is Streptomyces avidinii, Streptomyces colombiensis, Streptomyces lavendulae, Streptomyces roseochromogenus, Streptomyces spororaveus, Streptomyces sporoverrucosus, Streptomyces venezuelae, or Streptomyces xanthophaeus .

[0123] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 7. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 7. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 7. In some embodiments, the at least one microbial signaler is Streptomyces avidinii, Streptomyces cirratus, Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces spororaveus, Streptomyces subrutilus, Streptomyces venezuelae, or Streptomyces xanthophaeus.

[0124] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 8. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 8. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 8. In some embodiments, the at least one microbial signaler is Streptomyces avidinii,Streptomyces colombiensis, Streptomyces flaveus, Streptomyces goshikiensis, Streptomyces lavendulae, Streptomyces spororaveus, Streptomyces subrutilus, or Streptomyces venezuelae .

[0125] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 9. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 9. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 9. In some embodiments, the at least one microbial signaler is Streptomyces cinnamonensis, Streptomyces cirratus, Streptomyces goshikiensis, Streptomyces lavendulae, Streptomyces senoensis, Streptomyces sporoverrucosus, Streptomyces vinaceus, or Streptomyces virginiae.

[0126] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 10. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 10. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 10. In some embodiments, the at least one microbial signaler is Streptomyces cinnamonensis, Streptomyces flaveus, Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces spororaveus, Streptomyces venezuelae, Streptomyces virginiae, or Streptomyces xanthophaeus .

[0127] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 11. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 11. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 11. In some embodiments, the at least one microbial signaler is Streptomyces auratus, Streptomyces cinnamonensis, Streptomyces lavendulae, Streptomyces sioyaensis, Streptomyces spororaveus, Streptomyces verne, Streptomyces virginiae, or Streptomyces xanthophaeus .

[0128] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 12. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 12. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 12. In some embodiments, the at least one microbial signaler is Streptomyces cinnamonensis, Streptomyces flaveus, Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces spororaveus, Streptomyces venezuelae, Streptomyces virginiae, or Streptomyces xanthophaeus.

[0129] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at leastabout 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 13. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 13. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 13. In some embodiments, the at least one microbial signaler is Streptomyces badius, Streptomyces cyaneofuscatus, Streptomyces flavogriseus, Streptomyces griseus, Streptomyces lavendulae, Streptomyces mediolani, Streptomyces praecox, or Streptomyces pratensis.

[0130] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 14. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 14. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 14. In some embodiments, the at least one microbial signaler is Streptomyces avidinii, Streptomyces cirratus, Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces omiyaensis, Streptomyces spororaveus, Streptomyces subrutilus, or Streptomyces vinaceus.

[0131] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 15. In someembodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 15. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 15. In some embodiments, the at least one microbial signaler is Streptomyces cinnamonensis, Streptomyces flaveus, Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces vinaceus, Streptomyces virginiae, or Streptomyces xanthophaeus .

[0132] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 16. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 16. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 16. In some embodiments, the at least one microbial signaler is Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces spororaveus, Streptomyces subrutilus, Streptomyces venezuelae, Streptomyces virginiae, or Streptomyces xanthophaeus.

[0133] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 17. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 17. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 17. In some embodiments, the at least one microbial signaler is Streptomycescinnamonensis, Streptomyces flaveus, Streptomyces goshikiensis, Streptomyces lavendulae, Streptomyces nojiriensis, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces virginiae, or Streptomyces xanthophaeus .

[0134] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 18. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 18. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 18. In some embodiments, the at least one microbial signaler is Streptomyces colombiensis, Streptomyces flaveus, Streptomyces lavendulae, Streptomyces senoensis, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces vinaceus, Streptomyces virginiae, or Streptomyces xanthophaeus.

[0135] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 19. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 19. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 19. In some embodiments, the at least one microbial signaler is Streptomyces flaveus, Streptomyces lavendulae, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces vinaceus, Streptomyces virginiae, or Streptomyces xanthophaeus.

[0136] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 20. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 20. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 20. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces platensis, Streptomyces rimosus, or Streptomyces tubercidicus .

[0137] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 21. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 21. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 21. In some embodiments, Streptomyces angustmyceticus, Streptomyces catenulae, Streptomyces cinereus, Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces nigrescens, or Streptomyces sioyaensis.

[0138] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, atleast about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 22. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 22. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 22. In some embodiments, the at least one microbial signaler is Streptomyces atrolaccus, Streptomyces auratus, Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces sioyaensis, or Streptomyces tubercidicus .

[0139] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 23. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 23. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 23. In some embodiments, the at least one microbial signaler is Streptomyces atrolaccus, Streptomyces auratus, Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces sioyaensis, or Streptomyces tubercidicus.

[0140] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lietherebetween) sequence identity to one or more of the following SEQ ID NO: 24. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 24. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 24. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces atrolaccus, Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces nigrescens, Streptomyces sioyaensis, or Streptomyces tubercidicus .

[0141] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 25. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 25. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 25. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces platensis, Streptomyces rimosus, or Streptomyces tubercidicus.

[0142] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 26. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 26. In some embodiments, the at least one microbial signalercomprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 26. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces platensis, Streptomyces rimosus, or Streptomyces sioyaensis.

[0143] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 27. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 27. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 27. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces atrolaccus, Streptomyces chattanoogensis, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces sioyaensis, or Streptomyces tubercidicus .

[0144] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 28. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 28. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 28. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus,Streptomyces nigrescens, Streptomyces platensis, Streptomyces rimosus, or Streptomyces tubercidicus .

[0145] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 29. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 29. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 29. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces atrolaccus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces sioyaensis, or Streptomyces tubercidicus.

[0146] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 30. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 30. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 30. In some embodiments, the at least one microbial signaler is Streptomyces caniferus, Streptomyces decoyicus, Streptomyces glebosus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces ossamyceticus, or Streptomyces platensis.

[0147] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 31. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 31. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 31. In some embodiments, the at least one microbial signaler is Streptomyces angustmyceticus, Streptomyces catenulae, Streptomyces cinereus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces platensis, or Streptomyces tubercidicus .

[0148] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 32. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 32. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 32. In some embodiments, the at least one microbial signaler is Streptomyces argenteolus, Streptomyces atrolaccus, Streptomyces chattanoogensis, Streptomyces chrestomyceticus, Streptomyces coelicolor, Streptomyces lydicus, Streptomyces microsporus, Streptomyces nigrescens, Streptomyces rimosus, or Streptomyces sioyaensis.

[0149] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at leastabout 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 33. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 33. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 33. In some embodiments, the at least one microbial signaler is Streptomyces aquilus, Streptomyces caeruleatus, Streptomyces fagopyri, Streptomyces griseochromogenes, Streptomyces mirabilis, Streptomyces nojiriensis, Streptomyces pseudovenezuelae, Streptomyces viridochromogenes, or Streptomyces viridochromogenes .

[0150] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 34. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 34. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 34. In some embodiments, the at least one microbial signaler is Streptomyces aquilus, Streptomyces aureus, Streptomyces fagopyri, Streptomyces lutosisoli, Streptomyces minoensis, Streptomyces mirabilis, Streptomyces olivochromogenes, or Streptomyces rhizosphaerihabitans .

[0151] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 35. In someembodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 35. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 35. In some embodiments, the at least one microbial signaler is Streptomyces aquilus, Streptomyces fagopyri, Streptomyces griseoruber, Streptomyces lutosisoli, Streptomyces minoensis, Streptomyces mirabilis, Streptomyces olivochromogenes, or Streptomyces scabiei.

[0152] In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 70% (for example, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 97.5%, at least about 98%, at least about 98.1%, at least 98.2%, at least about 98.3%, at least about 98.4%, at least about 98.5%, at least about 98.6%, at least about 98.7%, at least about 98.8%, at least about 98.9%, at least about 99%, at least about 99.5%, or about 100% sequence identity, including all subranges and values that lie therebetween) sequence identity to one or more of the following SEQ ID NO: 36. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence of any one of the following SEQ ID NO: 36. In some embodiments, the at least one microbial signaler comprises a 16S nucleic acid sequence having at least about 97% sequence identity to SEQ ID NO: 36. In some embodiments, the at least one microbial signaler is Streptomyces cirratus, Streptomyces nojiriensis, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces verne, Streptomyces vinaceus, Streptomyces virginiae, or Streptomyces xanthophaeus .Methods of Preparing Compositions Comprising Microbial Signalers Disclosed Herein

[0153] The disclosure provides methods of producing a composition, the method comprising: bringing at least one target microbe in the physical proximity of any one or more of the microbial signalers disclosed herein. The disclosure provides methods of producing a composition, the method comprising: bringing at least one target microbe in the physical proximity of at least one microbial signaler belonging to the genus Streptomyces, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of the at least one target microbe.

[0154] In some embodiments, the methods disclosed herein comprises bringing the target microbe in contact with the at least one microbial signaler. In some embodiments, themethod comprises preparing a composition, comprising the target microbe and the at least one microbial signaler.

[0155] The isolation, identification, and culturing of the microbes of the present disclosure can be effected using standard microbiological techniques. Examples of such techniques may be found in Gerhardt, P. (ed.) Methods for General and Molecular Microbiology. American Society for Microbiology, Washington, D.C. (1994) and Lennette, E. H. (ed.) Manual of Clinical Microbiology, Third Edition. American Society for Microbiology, Washington, D.C. (1980), each of which is incorporated by reference.

[0156] Isolation can be effected by streaking the specimen on a solid medium (e.g., nutrient agar plates) to obtain a single colony, which is characterized by the phenotypic traits described herein (e.g., Gram positive / negative, capable of forming spores aerobically / anaerobically, cellular morphology, carbon source metabolism, acid / base production, enzyme secretion, metabolic secretions, etc.) and to reduce the likelihood of working with a culture which has become contaminated.

[0157] For example, for microbes of the disclosure, biologically pure isolates can be obtained through repeated subculture of biological samples, each subculture followed by streaking onto solid media to obtain individual colonies or colony forming units. Methods of preparing, thawing, and growing lyophilized bacteria are commonly known, for example, Ghema, R. L. and C. A. Reddy. 2007. Culture Preservation, p 1019-1033. In C. A. Reddy, T. J. Beveridge, J. A. Breznak, G. A. Marzluf, T. M. Schmidt, and L. R. Snyder, eds. American Society for Microbiology, Washington, D.C., 1033 pages; herein incorporated by reference. Thus freeze dried liquid formulations and cultures stored long term at -70° C in solutions containing glycerol are contemplated for use in providing formulations of the present disclosure.

[0158] The microbes of the present disclosure can be propagated in a liquid or solid medium under aerobic conditions, or alternatively anaerobic conditions. Medium for growing the bacterial strains of the present disclosure may include a carbon source, a nitrogen source, and inorganic salts, as well as specially required substances such as vitamins, amino acids, nucleic acids and the like. In some embodiments, the media comprises water and agar. Examples of suitable carbon sources which can be used for growing the microbes include, but are not limited to, starch, peptone, yeast extract, amino acids, sugars such as glucose, arabinose, mannose, glucosamine, maltose, and the like; salts of organic acids such as aceticacid, fumaric acid, adipic acid, propionic acid, citric acid, gluconic acid, malic acid, pyruvic acid, malonic acid and the like; alcohols such as ethanol and glycerol and the like; oil or fat such as soybean oil, rice bran oil, olive oil, corn oil, sesame oil. The amount of the carbon source added varies according to the kind of carbon source and is typically between 1 to 100 gram(s) per liter of medium. Preferably, glucose, starch, and / or peptone is contained in the medium as a major carbon source, at a concentration of 0.1-5% (W / V). Examples of suitable nitrogen sources which can be used for growing the bacterial strains of the present disclosure include, but are not limited to, amino acids, yeast extract, tryptone, beef extract, peptone, potassium nitrate, ammonium nitrate, ammonium chloride, ammonium sulfate, ammonium phosphate, ammonia or combinations thereof. The amount of nitrogen source varies according to the type of nitrogen source, typically between 0.1 to 30 gram(s) per liter of medium. The inorganic salts, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, magnesium chloride, ferric sulfate, ferrous sulfate, ferric chloride, ferrous chloride, manganous sulfate, manganous chloride, zinc sulfate, zinc chloride, cupric sulfate, calcium chloride, sodium chloride, calcium carbonate, sodium carbonate can be used alone or in combination. The amount of inorganic acid varies according to the kind of the inorganic salt, typically between 0.001 to 10 gram(s) per liter of medium. Examples of specially required substances include, but are not limited to, vitamins, nucleic acids, yeast extract, peptone, meat extract, malt extract, dried yeast and combinations thereof. Cultivation can be effected at a temperature, which allows the growth of the microbial strains, essentially, between 20°C and 46°C. In some embodiments, a temperature range is 30°C-39°C. For optimal growth, in some embodiments, the medium can be adjusted to Ph 6.0-7.4. It will be appreciated that commercially available media may also be used to culture the microbial strains, such as Nutrient Broth or Nutrient Agar available from Difco, Detroit, MI. It will be appreciated that cultivation time may differ depending on the type of culture medium used and the concentration of sugar as a major carbon source.

[0159] In some embodiments, cultivation lasts between about 24 to about 96 hours. In some embodiments, cultivation lasts longer than 96 hours, such as, for example, about 4 days, about 5 days, about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, or about 2 months. Microbial cells thus obtained are isolated using methods, which are well known in the art. Examples include, but are not limited to, membrane filtration and centrifugal separation. The Ph may be adjusted using sodium hydroxide and the like and the culture may be dried using a freeze dryer, until the water content becomes equal to 4% or less. Microbialco-cultures may be obtained by propagating each strain as described hereinabove. In some embodiments, microbial multi-strain cultures may be obtained by propagating two or more of the strains described hereinabove. It will be appreciated that the microbial strains may be cultured together when compatible culture conditions can be employed.

[0160] The disclosure provides compositions produced using any one of the methods of producing compositions disclosed herein.Methods of Enhancing Plant Growth Promoting Function of Target Microbes

[0161] The disclosure provides methods of enhancing a plant growth-promoting function of a target microbe, the method comprising: bringing the target microbe in the physical proximity of any one or more of the microbial signalers disclosed herein. The disclosure also provides methods of enhancing a plant growth-promoting function of a target microbe, the method comprising: bringing the target microbe in the physical proximity of at least one microbial signaler belonging to the genus Streptomyces disclosed herein.

[0162] In some embodiments, the methods comprise increasing the plant growthpromoting function of the target microbe by at least about 1% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the methods comprise increasing plant growthpromoting function of the target microbe by about 1% to about 900%, about 2% to about 800%, about 3% to about 700%, about 4% to about 600%, about 5% to about 500%, about 1% to about 100%, about 2% to about 200%, about 3% to about 300%, about 4% to about 400%, or by about 5% to about 1000%. In some embodiments, the methods comprise increasing plant growth-promoting function of the target microbe by about 10% to about 100%, about 10% to about 200%, about 10% to about 300%, about 10% to about 400%, about 10% to about 500%, about 10% to about 1000%, about 25% to about 500%, about 25% to about 1000%, about 50% to about 500%, or by about 50% to about 1000%, including all values and subranges that lie therebetween.In some embodiments, the plant growth-promoting function comprises: (a) plant pathogeninhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, (e) nitrogen fixing function, (f) a function of improving a plant’s nutrient acquisition, (f) production of plant growth hormones, or (g) any combination thereof.

[0163] In some embodiments, the methods disclosed herein enhance the plant pathogen-inhibiting function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the plant pathogen-inhibiting function of the target microbe by at least about 5%. In some embodiments, the methods comprise enhancing the plant pathogen-inhibiting function of the target microbe by about 1% to about 900%, about 2% to about 800%, about 3% to about 700%, about 4% to about 600%, about 5% to about 500%, about 1% to about 100%, about 2% to about 200%, about 3% to about 300%, about 4% to about 400%, or by about 5% to about 1000%. In some embodiments, the methods enhancing the plant pathogen-inhibiting function of the target microbe by about 10% to about 100%, about 10% to about 200%, about 10% to about 300%, about 10% to about 400%, about 10% to about 500%, about 10% to about 1000%, about 25% to about 500%, about 25% to about 1000%, about 50% to about 500%, or by about 50% to about 1000%, including all values and subranges that lie therebetween.

[0164] In some embodiments, the method disclosed herein enhance the zinc solubilizing function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lietherebetween). In some embodiments, the method enhances the zinc solubilizing function of the target microbe by at least about 5%. In some embodiments, the methods comprise enhancing the zinc solubilizing function of the target microbe by about 1% to about 900%, about 2% to about 800%, about 3% to about 700%, about 4% to about 600%, about 5% to about 500%, about 1% to about 100%, about 2% to about 200%, about 3% to about 300%, about 4% to about 400%, or by about 5% to about 1000%. In some embodiments, the methods comprise enhancing the zinc solubilizing function of the target microbe by about 10% to about 100%, about 10% to about 200%, about 10% to about 300%, about 10% to about 400%, about 10% to about 500%, about 10% to about 1000%, about 25% to about 500%, about 25% to about 1000%, about 50% to about 500%, or by about 50% to about 1000%, including all values and subranges that lie therebetween.

[0165] In some embodiments, the method disclosed herein enhance the phosphate solubilizing function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the phosphate solubilizing function of the target microbe by at least about 5%. In some embodiments, the methods comprise enhancing the phosphate solubilizing function of the target microbe by about 1% to about 900%, about 2% to about 800%, about 3% to about 700%, about 4% to about 600%, about 5% to about 500%, about 1% to about 100%, about 2% to about 200%, about 3% to about 300%, about 4% to about 400%, or by about 5% to about 1000%. In some embodiments, the methods comprise enhancing the phosphate solubilizing function of the target microbe by about 10% to about 100%, about 10% to about 200%, about 10% to about 300%, about 10% to about 400%, about 10% to about 500%, about 10% to about 1000%, about 25% to about 500%, about 25% to about 1000%, about 50% to about 500%, or by about 50% to about 1000%, including all values and subranges that lie therebetween.

[0166] In some embodiments, the method disclosed herein enhance the plant-growth promoting function of the target microbe under low nutrient conditions.Methods of Enhancing Nematicide Function of Target Microbes

[0167] In embodiments, provided herein are methods of enhancing nematicide function of a target microbe, the method comprising: bringing the target microbe in the physical proximity of any one or more of the microbial signalers disclosed herein. The disclosure also provides methods of enhancing a nematicide function of a target microbe, the method comprising: bringing the target microbe in the physical proximity of at least one microbial signaler belonging to the genus Streptomyces disclosed herein. In some embodiments, the microbial signaler and the target microbe are in contact (e.g., exposed to each other) prior to nematicide function. In some embodiments, the microbial signaler and the target microbe are physically mixed (e.g., as a composition) prior to nematicide function. In some embodiments, the microbial signaler and the target microbe are brought in physical proximity concurrently with nematicide function. In some embodiments, the microbial signaler and the target microbe are brought in physical proximity sequentially with nematicide function. In embodiments, the microbial signaler and target microbe are both applied to a seed. In embodiments, the microbial signaler and target microbe are applied to a plant. In embodiments, the microbial signaler and target microbe are both applied to soil near a seed and / or plant.

[0168] In some embodiments, the target microbe is Bacillus, Pseudomonas, Rhizobium, Bradyrhizobium, Kosakona, Trichoderma, Azotobacter, or Azospirillum or combinations thereof In some embodiments, the target microbe is Accharomyces cerevisiae, Agrobacterium radiobacter, Bacillus amyloliquefaciens, Bacillus amyloliquefaciens subspecies plantarum, Bacillus firmus, Bacillus licheniformis, Bacillus megaterium, Bacillus methylotrophicus, Bacillus pumilus, Bacillus spp., Bacillus subtilis, Bacillus subtilis, Bradyrhizobium japonicum, Bradyrhizobium-sp. (Coronilla), Bradyrhizobium-sp. (Lespedeza), Bradyrhizobium-sp. (Onobrychis), Endomycorrhizal spp., Gigaspora margarita, Glomus aggregatum, Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices, Glomus monosporus, Glomus mosseae, Laccaria laccata, Lactobacillus casei, Paraglomus brasilianum, Phanaerochaete chrysosporium, Pisolithus tinctorius, Pseudomonas, Rhizobium leguminosarum biovar trifolii , Rhizobium leguminosarum biovar viceae, Rhizobium loti, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon rubescens, Rhizopogon villosuli, Rhodopseudomonas palustris, Scleroderma cepa, Scleroderma citrinum, Streptomyce griseus, Trichoderma harzainum, and Tricoderma reesei, or combinations thereof. In some embodiments, the target microbe is Bacillus. In some embodiments, the target microbe is Bacillus spp. In some embodiments, the target microbe is Pseudomonas. In some embodiments,the target microbe is Rhizobium. In some embodiments, the target microbe is Bradyrhizobium . In some embodiments, the target microbe is Kosakonia. In some embodiments, the target microbe is Trichoderma. In some embodiments, the target microbe is Azotobacter. In some embodiments, the target microbe is Azospirillum. In some embodiments, the target microbes are Bacillus subtilis strain BUI 814, Bacillus amyloliquefaciens strain MB 1600, and Bradyrhizobium japonicum. In some embodiments, the target microbe is Bacillus firmus strain 1-1582. In some embodiments, the target microbes are Bacillus subtilis, Bacillus methylotrophicus and Bacillus licheniformis. In some embodiments, the target microbes are Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and Bacillus coagulans. In some embodiments, the target microbes are Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus coagulans Endomycorrhizal fungi, and Trichoderma harzianum. In some embodiments, the target microbes are Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and Bacillus subtilis. In some embodiments, the target microbe is Bacillus amyloliquefaciens, Glomus aggregatum, Glomus etunicatum, Glomus clarum, Glomus deserticola, Glomus intraradices, Glomus monosporus, Glomus mosseae, Gigaspora margarita, Paraglomus brasilianum, Laccaria laccata, Pisolithus tinctorius, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon rubescens, Rhizopogon villosuli, Scleroderma cepa, and Scleroderma citrinum. In some embodiments, the target microbe is Bacillus amyloliquefaciens. In some embodiments, the target microbes are Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and Bacillus subtilis. In some embodiments, the target microbe is Bacillus amyloliquefaciens strain FZB45. In some embodiments, the target microbe is Bacillus amyloliquefaciens subspecies plantarum and Bacillus subtilis. In some embodiments, the target microbe is Rhizobium leguminosarum biovar trifolii. In some embodiments, the target microbe is Bradyrhizobium-sp. (Coronilla). In some embodiments, the target microbes are Bacillus amyloliquefaciens subspecies plantarum and Bacillus subtilis. In some embodiments, the target microbe is Agrobacterium radiobacter isolate 84. In some embodiments, the target microbe is Bradyrhizobium-sp. (Lespedeza). In some embodiments, the target microbe is Bradyrhizobium japonicum WB74. In some embodiments, the target microbe is Rhizobium loti. In some embodiments, the target microbes are Lactobacillus casei, Lactobacillus casei, Accharomyces cerevisiae, Bacillus subtilis, Streptomyce griseus, and Rhodopseudomonas palustris. In some embodiments, the target microbe is Pseudomonas. In some embodiments, the target microbe is Bradyrhizobium-sp.(Onobrychis) strain XHH1. In some embodiments, the target microbe is Bradyrhizobium japonicum strain WB74. In some embodiments, the target microbe is Trichoderma harzainum.

[0169] In embodiments, the methods provided herein enhance the nematicide function of target microbes against one or more nematodes. In some embodiments, the methods provided herein enhance nematicide function against e.g., Root-knot nematode (Meloidogyne spp.), Burrowing nematode (Radopholus similis . Cyst nematode Heterodera spp. and / or Globodera spp.), Reniform nematode (Rotylenchulus reniformis), False root-knot nematode (Nacobbus spp.), Spiral nematode (Helicotylenchus spp.), Sting nematode (Belonolaimus spp.), or Root lesion nematode (Pratylenchus spp.), or combinations thereof. Exemplary nematode genera may include: Pratylenchus (e.g., P. penetrans, P. brachyurus, P. scribneri, and P. zaea) also known as root lesion nematodes (RLN); Meloidogyne (e.g., M. incognita, M. enterlobii, M. javanica, M. japla, and M. arenaria) also known as root knot nematodes (RKN); Rotylenchulus (e.g., R reniformis) also known as reniform nematodes; Hoplolaimus (H. columbus also known as lance nematodes, and; Belonolaimus (B. longicaudatus) also known as sting, stunt, and pin nematodes. Exemplary nematode species may include Heterodera glycines (H. glycines), also known as soybean cyst nematodes (SCN). In embodiments, the methods provided herein enhance the nematicide function of target microbes against one or more nematode selected from the group consisting of: P. penetrans, P. brachyurus, P. scribneri, P. zaea, M. incognita, M. enterlobii, M. javanica, M. japla, M. arenaria, R. reniformis, H. columbus, B. longicaudatus, and H. glycines, and combinations thereof

[0170] In some embodiments, the methods comprise increasing the nematicide function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise increasing the nematicide function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise increasing the nematicide function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about100%. In some embodiments, the methods comprise increasing the nematicide function of the target microbe by at least about 1% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween).

[0171] In some embodiments, the nematicide function comprises: (a) plant root biomass function, (b) shoot biomass function, (c) soybean cyst nematode (SCN) female counts function, (d) SCN egg counts function, (e) root lesion nematodes (RLN) female counts function, (f) RLN egg counts function, (g) root knot nematode (RKN) female counts function, (h) RKN egg counts function, (i) root lesion counts function, (j) root galling counts function, or (k) any combinations thereof. In some embodiments, enhancing nematicide function comprises one or more of increasing plant root biomass, increasing shoot biomass, decreasing SCN female counts, decreasing SCN egg counts, decreasing RLN female counts, decreasing RLN egg counts, decreasing RKN female counts, decreasing RKN egg counts, decreasing root lesion counts, and decreasing root galling counts..

[0172] In some embodiments, the methods comprise enhancing the plant root biomass function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the plant root biomass function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the plant root biomass function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed hereinenhance the plant root biomass function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the plant root biomass function of the target microbe by at least about 5%. In some embodiments, enhancing plant root biomass function of the target microbe comprises increasing plant root biomass.

[0173] In some embodiments, the methods comprise enhancing the shoot biomass function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the shoot biomass function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the shoot biomass function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the shoot biomass function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lietherebetween). In some embodiments, the method enhances the shoot biomass function of the target microbe by at least about 5%. In some embodiments, enhancing shoot biomass function of the target microbe comprises increasing shoot biomass.

[0174] In some embodiments, the methods comprise enhancing the SCN female counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 500%. In some embodiments, the methods comprise enhancing the SCN female counts function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the SCN female counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the SCN female counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the SCN female counts function of the target microbe by at least about 5%. In some embodiments, enhancing SCN female counts function of the target microbe comprises decreasing SCN female counts.

[0175] In some embodiments, the methods comprise enhancing the SCN egg counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, themethods comprise enhancing the SCN egg counts of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the SCN egg counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the SCN egg counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the SCN egg counts function of the target microbe by at least about 5%. In some embodiments, enhancing SCN egg counts function of the target microbe comprises decreasing SCN egg counts.

[0176] In some embodiments, the methods comprise enhancing the RLN female counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the RLN female counts function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the RLN female counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the RLN female counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the RLN female counts function of the target microbe by at least about 5%. In some embodiments, enhancing RLN female counts function of the target microbe comprises decreasing RLN female counts.

[0177] In some embodiments, the methods comprise enhancing the RLN egg counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the RLN egg counts function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the RLN egg counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the RLN egg counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, atleast about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the RLN egg counts function of the target microbe by at least about 5%. In some embodiments, enhancing the RLN egg counts function of the target microbe comprises decreasing the RLN egg counts.

[0178] In some embodiments, the methods disclosed herein enhance the RKN female counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the RKN female counts function of the target microbe by at least about 5%. In some embodiments, enhancing the RKN female counts function of the target microbe comprises decreasing the RKN female counts.

[0179] In some embodiments, the methods comprise enhancing the RKN egg counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the RKN egg counts function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the RKN egg counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the RKN egg counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the RKN egg counts function of the target microbe by at least about 5%. In some embodiments, enhancing the RKN egg counts function of the target microbe comprises decreasing the RKN egg counts function.

[0180] In some embodiments, the methods comprise enhancing the root lesion counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the root lesion counts function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the root lesion counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the root lesion counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the root lesion counts function on of the target microbe by at least about 5%. In some embodiments, enhancingthe root lesion counts function of the target microbe comprises decreasing the root lesion counts.

[0181] In some embodiments, the methods comprise enhancing the root galling counts function of the target microbe from about 1% to about 900%, from about 2% to about 800%, from about 3% to about 700%, from about 4% to about 600%, from about 5% to about 500%, from about 1% to about 100%, from about 2% to about 200%, from about 3% to about 300%, from about 4% to about 400%, or from about 5% to about 1000%. In some embodiments, the methods comprise enhancing the root galling counts function of the target microbe from about 10% to about 100%, from about 10% to about 200%, from about 10% to about 300%, from about 10% to about 400%, from about 10% to about 500%, from about 10% to about 1000%, from about 25% to about 500%, from about 25% to about 1000%, from about 50% to about 500%, or from about 50% to about 1000%, including all values and subranges that lie therebetween. In some embodiments, the methods comprise enhancing the root galling counts function of the target microbe from about 10% to about 25%, from about 25% to about 50%, from about 25% to about 100%, from about 50% to about 100%, from about 75% to about 100%, or from about 90% to about 100%. In some embodiments, the methods disclosed herein enhance the root galling counts function of the target microbe by at least about 2% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 100%, at least about 200%, at least about 300%, at least about 400%, at least about 500%, at least about 600%, at least about 700%, at least about 800%, at least about 900% or at least about 1000%, including all values and subranges that lie therebetween). In some embodiments, the method enhances the root galling counts function of the target microbe by at least about 5%. In some embodiments, enhancing the root galling counts function of the target microbe comprises decreasing root galling counts.Microbial Compositions

[0182] The disclosure provides microbial compositions comprising any one or more of the microbial signalers disclosed herein and / or compositions disclosed herein. In some embodiments, the microbial compositions may further comprise suitable carrier and other additives. In some embodiments, the microbial compositions of the present disclosure are solid.Where solid compositions are used, it may be desired to include one or more carrier materials including, but not limited to: mineral earths such as silicas, talc, kaolin, limestone, chalk, clay, dolomite, diatomaceous earth; calcium sulfate; magnesium sulfate; magnesium oxide; zeolites, calcium carbonate; magnesium carbonate; trehalose; chitosan; shellac; and starch.

[0183] In some embodiments, the microbial compositions of the present disclosure are liquid. In further embodiments, the liquid comprises a solvent that may include water or an alcohol or a saline or carbohydrate solution, and other plant-safe solvents. In some embodiments, the microbial compositions of the present disclosure include binders such as plant-safe polymers, carboxymethylcellulose, starch, polyvinyl alcohol, and the like.

[0184] In some embodiments, the microbial compositions of the present disclosure comprise thickening agents such as silica, clay, natural extracts of seeds or seaweed, synthetic derivatives of cellulose, guar gum, locust bean gum, alginates, and methylcelluloses. In some embodiments, the microbial compositions comprise anti-settling agents such as modified starches, polyvinyl alcohol, xanthan gum, and the like.

[0185] In some embodiments, the microbial compositions of the present disclosure comprise colorants including organic chromophores classified as nitroso; nitro; azo, including monoazo, bisazo and polyazo; acridine, anthraquinone, azine, diphenylmethane, indamine, indophenol, methine, oxazine, phthalocyanine, thiazine, thiazole, triarylmethane, xanthene. In some embodiments, the microbial compositions of the present disclosure comprise trace nutrients such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc. In some embodiments, the microbial compositions comprise dyes, both natural and artificial.

[0186] In some embodiments, the microbial compositions of the present disclosure may include combinations of fungal spores and bacterial spores, fungal spores and bacterial vegetative cells, fungal vegetative cells and bacterial spores, fungal vegetative cells and bacterial vegetative cells. In some embodiments, compositions of the present disclosure comprise bacteria only in the form of spores. In some embodiments, compositions of the present disclosure comprise bacteria only in the form of vegetative cells. In some embodiments, compositions of the present disclosure comprise bacteria in the absence of fungi. In some embodiments, compositions of the present disclosure comprise fungi in the absence of bacteria. In some embodiments, compositions of the present disclosure comprise viable but non- culturable (VBNC) bacteria and / or fungi. In some embodiments, compositions of the present disclosure comprise bacteria and / or fungi in a quiescent state. In some embodiments,compositions of the present disclosure include dormant bacteria and / or fungi. Bacterial spores may include endospores and akinetes. Fungal spores may include statismospores, ballistospores, autospores, aplanospores, zoospores, mitospores, megaspores, microspores, meiospores, chlamydospores, urediniospores, teliospores, oospores, carpospores, tetraspores, sporangiospores, zygospores, ascospores, basidiospores, ascospores, and asciospores.

[0187] In some embodiments, the microbial compositions of the present disclosure comprise a plant-safe virucide, parasiticide, bacteriocide, fungicide, biopesticide, or nematicide. In some embodiments, microbial compositions of the present disclosure comprise one or more oxygen scavengers, denitrifies, nitrifiers, heavy metal chelators, and / or dechlorinators; and combinations thereof.

[0188] In some embodiments, microbial compositions of the present disclosure comprise one or more preservatives. The preservatives may be in liquid or gas formulations. The preservatives may be selected from one or more of monosaccharide, disaccharide, trisaccharide, polysaccharide, acetic acid, ascorbic acid, calcium ascorbate, erythorbic acid, iso-ascorbic acid, erythrobic acid, potassium nitrate, sodium ascorbate, sodium erythorbate, sodium iso-ascorbate, sodium nitrate, sodium nitrite, nitrogen, benzoic acid, calcium sorbate, ethyl lauroyl arginate, methyl- / ?-hydroxy benzoate, methyl paraben, potassium acetate, potassium benzoiate, potassium bisulphite, potassium diacetate, potassium lactate, potassium metabisulphite, potassium sorbate, propyl - -hydroxy benzoate, propyl paraben, sodium acetate, sodium benzoate, sodium bisulphite, sodium nitrite, sodium diacetate, sodium lactate, sodium metabisulphite, sodium salt of methyl- / ?-hydroxy benzoic acid, sodium salt of propyl- -hydroxy benzoic acid, sodium sulphate, sodium sulfite, sodium dithionite, sulphurous acid, calcium propionate, dimethyl dicarbonate, natamycin, potassium sorbate, potassium bisulfite, potassium metabisulfite, propionic acid, sodium diacetate, sodium propionate, sodium sorbate, sorbic acid, ascorbic acid, ascorbyl palmitate, ascorbyl stearate, butylated hydro-xyanisole, butylated hydroxytoluene (BHT), butylated hydroxyl anisole (BHA), citric acid, citric acid esters of mono- and / or diglycerides, L-cysteine, L-cysteine hydrochloride, gum guaiacum, gum guaiac, lecithin, lecithin citrate, monoglyceride citrate, monoisopropyl citrate, propyl gallate, sodium metabisulphite, tartaric acid, tertiary butyl hydroquinone, stannous chloride, thiodipropionic acid, dilauryl thiodipropionate, distearyl thiodipropionate, ethoxyquin, sulfur dioxide, formic acid, or tocopherol(s).

[0189] In some embodiments, microbial compositions of the present disclosure comprise one or more non-plant food ingredients. Exemplary non-plant food ingredientsinclude amino acids, vitamins, sugars, salts, and ions. In some embodiments, microbial compositions of the present disclosure comprise one or more culture mediums know to an ordinary person of skill in the art. Culture media may be liquid, solid, or semisolid, for example. Culture media may be for selection, enrichment, storage, or any other purpose used in the art.

[0190] In some embodiments, microbial compositions of the present disclosure comprise one or more additional compounds. Additional compounds may provide plant pathogen-inhibiting function. In some embodiments, one or more additional compounds may enhance the nematicide function of commercial products containing target microbes. Exemplary compounds include, but are not limited to, clothianidin and fluazaindolizine. In some embodiments, microbial compositions comprise clothianidin. In some embodiments, microbial compositions comprise fluazaindolizine.

[0191] In some embodiments, the microbial compositions are shelf stable in a refrigerator (35-40°F) for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 days. In some embodiments, the microbial compositions are shelf stable in a refrigerator (35-40°F) for a period of about 1-10 days, about 5-20 days, about 10-60 days, about 1-60 days, about 30-60 days, about 5-60 days, or about 5-50 days. In some embodiments, the microbial compositions are shelf stable in a refrigerator (35-40°F) for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 weeks. In some embodiments, the microbial compositions are shelf stable in a refrigerator (35- 40°F) for a period of about 1-10 weeks, about 5-20 weeks, about 10-60 weeks, about 1-60 weeks, about 30-60 weeks, about 5-60 weeks, or about 5-50 weeks. In some embodiments, the microbial compositions are shelf stable in a refrigerator (35-40°F) for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 years. In some embodiments, the microbial compositions are shelf stable in a refrigerator (35-40°F) for a period of about 1-10 years, about 5-20 years, about 10- 60 years, about 1-60 years, about 30-60 years, about 5-60 years, or about 5-50 years.

[0192] In some embodiments, the microbial compositions are shelf stable at room temperature (68-72°F) or between 50-77°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 days. In some embodiments, the microbial compositions are shelf stable at room temperature (68-72°F) or between 50-77°F for a period of about 1-10 days, about 5-20 days, about 10-60 days, about 1-60 days, about 30-60 days, about 5-60 days, or about 5-50 days. In some embodiments, the microbial compositions are shelf stable at room temperature (68-72°F) or between 50-77°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 weeks. In some embodiments, the microbial compositions are shelf stable at room temperature (68-72°F) or between 50-77°F for a period of about 1-10 weeks, about 5-20 weeks, about 10-60 weeks, about 1-60 weeks, about 30-60 weeks, about 5-60 weeks, or about 5-50 weeks. In some embodiments, the microbial compositions are shelf stable at room temperature (68-72°F) or between 50-77°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 years. In some embodiments, the microbial compositions are shelf stable at room temperature (68-72°F) or between 50-77°F for a period of about 1-10 years, about 5-20 years, about 10-60 years, about 1-60 years, about 30-60 years, about 5-60 years, or about 5-50 years.

[0193] In some embodiments, the microbial compositions are shelf stable at -23-35°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 days. In some embodiments, the microbial compositions are shelf stable at -23-35°F for a period of about 1-10 days, about 5-20 days, about 10-60 days, about 1-60 days, about 30-60 days, about 5-60 days, or about 5-50 days. In some embodiments, the microbial compositions are shelf stable at -23-35°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 weeks. In some embodiments, the microbial compositions are shelf stable at -23-35°F for a period of about 1-10 weeks, about 5-20 weeks, about 10-60 weeks, about 1-60 weeks, about 30-60 weeks, about 5-60 weeks, or about 5-50 weeks. In some embodiments, the microbial compositions are shelf stable at -23 -35 °F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,55, 56, 57, 58, 59, or 60 years. In some embodiments, the microbial compositions are shelf stable at -23-35°F for a period of about 1-10 years, about 5-20 years, about 10-60 years, about 1-60 years, about 30-60 years, about 5-60 years, or about 5-50 years.

[0194] In some embodiments, the microbial compositions are shelf stable at 77-100°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 days. In some embodiments, the microbial compositions are shelf stable at 77-100°F for a period of about 1-10 days, about 5-20 days, about 10-60 days, about 1-60 days, about 30-60 days, about 5-60 days, or about 5-50 days. In some embodiments, the microbial compositions are shelf stable at 77-100°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52,53, 54, 55, 56, 57, 58, 59, or 60 weeks. In some embodiments, the microbial compositions are shelf stable at 77-100°F for a period of about 1-10 weeks, about 5-20 weeks, about 10-60 weeks, about 1-60 weeks, about 30-60 weeks, about 5-60 weeks, or about 5-50 weeks. In some embodiments, the microbial compositions are shelf stable at 77-100°F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, or 60 years. In some embodiments, the microbial compositions are shelf stable at 77-100°F for a period of about 1-10 years, about 5-20 years, about 10-60 years, about 1-60 years, about 30-60 years, about 5-60 years, or about 5-50 years.

[0195] In some embodiments, the microbial compositions are shelf stable at 101-213 °F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 days. In some embodiments, the microbial compositions are shelf stable at 101-213°F for a period of about 1-10 days, about 5-20 days, about 10-60 days, about 1-60 days, about 30-60 days, about 5-60 days, or about 5-50 days. In some embodiments, the microbial compositions are shelf stable at 101 -213 °F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 weeks. In some embodiments, the microbial compositions are shelf stable at 101-213°F for a period of about 1-10 weeks, about 5-20 weeks, about 10-60 weeks, about 1-60 weeks, about 30-60 weeks, about 5-60 weeks, or about 5-50 weeks. In someembodiments, the microbial compositions are shelf stable at 101 -213 °F for a period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 years. In some embodiments, the microbial compositions are shelf stable at 101-213°F for a period of about 1-10 years, about 5-20 years, about 10-60 years, about 1-60 years, about 30-60 years, about 5-60 years, or about 5-50 years.

[0196] In some embodiments, the microbial compositions of the present disclosure are shelf stable at refrigeration temperatures (35-40°F), at room temperature (68-72°F), between 50-77°F, between -23-35°F, between 70-100°F, or between 101-213°F for a period of about 1 to 100, about 1 to 95, about 1 to 90, about 1 to 85, about 1 to 80, about 1 to 75, about 1 to 70, about 1 to 65, about 1 to 60, about 1 to 55, about 1 to 50, about 1 to 45, about 1 to 40, about 1 to 35, about 1 to 30, about 1 to 25, about 1 to 20, about 1 to 15, about 1 to 10, about 1 to 5, about 5 to 100, about 5 to 95, about 5 to 90, about 5 to 85, about 5 to 80, about 5 to 75, about 5 to 70, about 5 to 65, about 5 to 60, about 5 to 55, about 5 to 50, about 5 to 45, about 5 to 40, about 5 to 35, about 5 to 30, about 5 to 25, about 5 to 20, about 5 to 15, about 5 to 10, about 10 to 100, about 10 to 95, about 10 to 90, about 10 to 85, about 10 to 80, about 10 to 75, about 10 to 70, about 10 to 65, about 10 to 60, about 10 to 55, about 10 to 50, about 10 to 45, about 10 to 40, about 10 to 35, about 10 to 30, about 10 to 25, about 10 to 20, about 10 to 15, about 15 to 100, about 15 to 95, about 15 to 90, about 15 to 85, about 15 to 80, about 15 to 75, about 15 to 70, about 15 to 65, about 15 to 60, about 15 to 55, about 15 to 50, about 15 to 45, about 15 to 40, about 15 to 35, about 15 to 30, about 15 to 25, about 15 to 20, about 20 to 100, about 20 to 95, about 20 to 90, about 20 to 85, about 20 to 80, about 20 to 75, about 20 to 70, about 20 to 65, about 20 to 60, about 20 to 55, about 20 to 50, about 20 to 45, about 20 to 40, about 20 to 35, about 20 to 30, about 20 to 25, about 25 to 100, about 25 to 95, about 25 to 90, about 25 to 85, about 25 to 80, about 25 to 75, about 25 to 70, about 25 to 65, about 25 to 60, about 25 to 55, about 25 to 50, about 25 to 45, about 25 to 40, about 25 to 35, about 25 to 30, about 30 to 100, about 30 to 95, about 30 to 90, about 30 to 85, about 30 to 80, about 30 to 75, about 30 to 70, about 30 to 65, about 30 to 60, about 30 to 55, about 30 to 50, about 30 to 45, about 30 to 40, about 30 to 35, about 35 to 100, about 35 to 95, about 35 to 90, about 35 to 85, about 35 to 80, about 35 to 75, about 35 to 70, about 35 to 65, about 35 to 60, about 35 to 55, about 35 to 50, about 35 to 45, about 35 to 40, about 40 to 100, about 40 to 95, about 40 to 90, about 40 to 85, about 40 to 80, about 40 to 75, about 40 to 70, about 40 to 65, about 40 to 60, about 40 to 55, about 40 to 50, about 40 to 45, about 45 to 100, about 45 to 95, about 45 to 90, about 45to 85, about 45 to 80, about 45 to 75, about 45 to 70, about 45 to 65, about 45 to 60, about 45 to 55, about 45 to 50, about 50 to 100, about 50 to 95, about 50 to 90, about 50 to 85, about 50 to 80, about 50 to 75, about 50 to 70, about 50 to 65, about 50 to 60, about 50 to 55, about 55 to 100, about 55 to 95, about 55 to 90, about 55 to 85, about 55 to 80, about 55 to 75, about 55 to 70, about 55 to 65, about 55 to 60, about 60 to 100, about 60 to 95, about 60 to 90, about 60 to 85, about 60 to 80, about 60 to 75, about 60 to 70, about 60 to 65, about 65 to 100, about 65 to 95, about 65 to 90, about 65 to 85, about 65 to 80, about 65 to 75, about 65 to 70, about 70 to 100, about 70 to 95, about 70 to 90, about 70 to 85, about 70 to 80, about 70 to 75, about 75 to 100, about 75 to 95, about 75 to 90, about 75 to 85, about 75 to 80, about 80 to 100, about 80 to 95, about 80 to 90, about 80 to 85, about 85 to 100, about 85 to 95, about 85 to 90, about 90 to 100, about 90 to 95, or 95 to 100 weeks.

[0197] In some embodiments, the microbial compositions of the present disclosure are shelf stable at refrigeration temperatures (35-40°F), at room temperature (68-72°F), between 50-77°F, between -23 -35 °F, between 70-100°F, or between 101-213°F for a period of 1 to 100, 1 to 95, 1 to 90, 1 to 85, 1 to 80, 1 to 75, 1 to 70, 1 to 65, 1 to 60, 1 to 55, 1 to 50, 1 to 45, 1 to 40, 1 to 35, 1 to 30, 1 to 25, 1 to 20, 1 to 15, 1 to 10, 1 to 5, 5 to 100, 5 to 95, 5 to 90, 5 to 85, 5 to 80, 5 to 75, 5 to 70, 5 to 65, 5 to 60, 5 to 55, 5 to 50, 5 to 45, 5 to 40, 5 to 35, 5 to 30, 5 to 25, 5 to 20, 5 to 15, 5 to 10, 10 to 100, 10 to 95, 10 to 90, 10 to 85, 10 to 80, 10 to 75, 10 to 70, 10 to 65, 10 to 60, 10 to 55, 10 to 50, 10 to 45, 10 to 40, 10 to 35, 10 to 30, 10 to 25, 10 to 20, 10 to 15, 15 to 100, 15 to 95, 15 to 90, 15 to 85, 15 to 80, 15 to 75, 15 to 70, 15 to 65, 15 to 60, 15 to 55, 15 to 50, 15 to 45, 15 to 40, 15 to 35, 15 to 30, 15 to 25, 15 to 20, 20 to 100, 20 to 95, 20 to 90, 20 to 85, 20 to 80, 20 to 75, 20 to 70, 20 to 65, 20 to 60, 20 to 55, 20 to 50, 20 to 45, 20 to 40, 20 to 35, 20 to 30, 20 to 25, 25 to 100, 25 to 95, 25 to 90, 25 to 85, 25 to 80, 25 to 75, 25 to 70, 25 to 65, 25 to 60, 25 to 55, 25 to 50, 25 to 45, 25 to 40, 25 to 35, 25 to 30, 30 to 100, 30 to 95, 30 to 90, 30 to 85, 30 to 80, 30 to 75, 30 to 70, 30 to 65, 30 to 60, 30 to 55, 30 to 50, 30 to 45, 30 to 40, 30 to 35, 35 to 100, 35 to 95, 35 to 90, 35 to 85, 35 to 80, 35 to 75, 35 to 70, 35 to 65, 35 to 60, 35 to 55, 35 to 50, 35 to 45, 35 to 40, 40 to 100, 40 to 95, 40 to 90, 40 to 85, 40 to 80, 40 to 75, 40 to 70, 40 to 65, 40 to 60, 40 to 55, 40 to 50, 40 to 45, 45 to 100, 45 to 95,45 to 90, 45 to 85, 45 to 80, 45 to 75, 45 to 70, 45 to 65, 45 to 60, 45 to 55, 45 to 50, 50 to 100,50 to 95, 50 to 90, 50 to 85, 50 to 80, 50 to 75, 50 to 70, 50 to 65, 50 to 60, 50 to 55, 55 to 100,55 to 95, 55 to 90, 55 to 85, 55 to 80, 55 to 75, 55 to 70, 55 to 65, 55 to 60, 60 to 100, 60 to 95,60 to 90, 60 to 85, 60 to 80, 60 to 75, 60 to 70, 60 to 65, 65 to 100, 65 to 95, 65 to 90, 65 to 85, 65 to 80, 65 to 75, 65 to 70, 70 to 100, 70 to 95, 70 to 90, 70 to 85, 70 to 80, 70 to 75, 75 to100, 75 to 95, 75 to 90, 75 to 85, 75 to 80, 80 to 100, 80 to 95, 80 to 90, 80 to 85, 85 to 100, 85 to 95, 85 to 90, 90 to 100, 90 to 95, or 95 to 100 weeks.

[0198] In some embodiments, the microbial compositions of the present disclosure are shelf stable at refrigeration temperatures (35-40°F), at room temperature (68-72°F), between 50-77°F, between -23-35°F, between 70-100°F, or between 101-213°F for a period of about 1 to 36, about 1 to 34, about 1 to 32, about 1 to 30, about 1 to 28, about 1 to 26, about 1 to 24, about 1 to 22, about 1 to 20, about 1 to 18, about 1 to 16, about 1 to 14, about 1 to 12, about 1 to 10, about 1 to 8, about 1 to 6, about 1 one 4, about 1 to 2, about 4 to 36, about 4 to 34, about 4 to 32, about 4 to 30, about 4 to 28, about 4 to 26, about 4 to 24, about 4 to 22, about 4 to 20, about 4 to 18, about 4 to 16, about 4 to 14, about 4 to 12, about 4 to 10, about 4 to 8, about 4 to 6, about 6 to 36, about 6 to 34, about 6 to 32, about 6 to 30, about 6 to 28, about 6 to 26, about 6 to 24, about 6 to 22, about 6 to 20, about 6 to 18, about 6 to 16, about 6 to 14, about 6 to 12, about 6 to 10, about 6 to 8, about 8 to 36, about 8 to 34, about 8 to 32, about 8 to 30, about 8 to 28, about 8 to 26, about 8 to 24, about 8 to 22, about 8 to 20, about 8 to 18, about 8 to 16, about 8 to 14, about 8 to 12, about 8 to 10, about 10 to 36, about 10 to 34, about 10 to 32, about 10 to 30, about 10 to 28, about 10 to 26, about 10 to 24, about 10 to 22, about 10 to20, about 10 to 18, about 10 to 16, about 10 to 14, about 10 to 12, about 12 to 36, about 12 to34, about 12 to 32, about 12 to 30, about 12 to 28, about 12 to 26, about 12 to 24, about 12 to22, about 12 to 20, about 12 to 18, about 12 to 16, about 12 to 14, about 14 to 36, about 14 to34, about 14 to 32, about 14 to 30, about 14 to 28, about 14 to 26, about 14 to 24, about 14 to22, about 14 to 20, about 14 to 18, about 14 to 16, about 16 to 36, about 16 to 34, about 16 to32, about 16 to 30, about 16 to 28, about 16 to 26, about 16 to 24, about 16 to 22, about 16 to20, about 16 to 18, about 18 to 36, about 18 to 34, about 18 to 32, about 18 to 30, about 18 to28, about 18 to 26, about 18 to 24, about 18 to 22, about 18 to 20, about 20 to 36, about 20 to34, about 20 to 32, about 20 to 30, about 20 to 28, about 20 to 26, about 20 to 24, about 20 to22, about 22 to 36, about 22 to 34, about 22 to 32, about 22 to 30, about 22 to 28, about 22 to26, about 22 to 24, about 24 to 36, about 24 to 34, about 24 to 32, about 24 to 30, about 24 to28, about 24 to 26, about 26 to 36, about 26 to 34, about 26 to 32, about 26 to 30, about 26 to28, about 28 to 36, about 28 to 34, about 28 to 32, about 28 to 30, about 30 to 36, about 30 to34, about 30 to 32, about 32 to 36, about 32 to 34, or about 34 to 36 months.

[0199] In some embodiments, the microbial compositions of the present disclosure are shelf stable at refrigeration temperatures (35-40°F), at room temperature (68-72°F), between 50-77°F, between -23-35°F, between 70-100°F, or between 101-213°F for a period of 1 to 36,1 to 34, 1 to 32, 1 to 30, 1 to 28, 1 to 26, 1 to 24, 1 to 22, 1 to 20, 1 to 18, 1 to 16, 1 to 14, 1 to 12, 1 to 10, 1 to 8, 1 to 6, 1 to 4, 1 to 2, 4 to 36, 4 to 34, 4 to 32, 4 to 30, 4 to 28, 4 to 26, 4 to 24, 4 to 22, 4 to 20, 4 to 18, 4 to 16, 4 to 14, 4 to 12, 4 to 10, 4 to 8, 4 to 6, 6 to 36, 6 to 34, 6 to 32, 6 to 30, 6 to 28, 6 to 26, 6 to 24, 6 to 22, 6 to 20, 6 to 18, 6 to 16, 6 to 14, 6 to 12, 6 to 10, 6 to 8, 8 to 36, 8 to 34, 8 to 32, 8 to 30, 8 to 28, 8 to 26, 8 to 24, 8 to 22, 8 to 20, 8 to 18, 8 to 16, 8 to 14, 8 to 12, 8 to 10, 10 to 36, 10 to 34, 10 to 32, 10 to 30, 10 to 28, 10 to 26, 10 to 24, 10 to 22, 10 to 20, 10 to 18, 10 to 16, 10 to 14, 10 to 12, 12 to 36, 12 to 34, 12 to 32, 12 to30, 12 to 28, 12 to 26, 12 to 24, 12 to 22, 12 to 20, 12 to 18, 12 to 16, 12 to 14, 14 to 36, 14 to34, 14 to 32, 14 to 30, 14 to 28, 14 to 26, 14 to 24, 14 to 22, 14 to 20, 14 to 18, 14 to 16, 16 to36, 16 to 34, 16 to 32, 16 to 30, 16 to 28, 16 to 26, 16 to 24, 16 to 22, 16 to 20, 16 to 18, 18 to36, 18 to 34, 18 to 32, 18 to 30, 18 to 28, 18 to 26, 18 to 24, 18 to 22, 18 to 20, 20 to 36, 20 to34, 20 to 32, 20 to 30, 20 to 28, 20 to 26, 20 to 24, 20 to 22, 22 to 36, 22 to 34, 22 to 32, 22 to30, 22 to 28, 22 to 26, 22 to 24, 24 to 36, 24 to 34, 24 to 32, 24 to 30, 24 to 28, 24 to 26, 26 to36, 26 to 34, 26 to 32, 26 to 30, 26 to 28, 28 to 36, 28 to 34, 28 to 32, 28 to 30, 30 to 36, 30 to34, 30 to 32, 32 to 36, 32 to 34, or 34 to 36 months.

[0200] In some embodiments, the microbial compositions of the present disclosure are shelf stable at refrigeration temperatures (35-40°F), at room temperature (68-72°F), between 50-77°F, between -23-35°F, between 70-100°F, or between 101-213°F for a period of about 1 to 36, about 1 to 34, about 1 to 32, about 1 to 30, about 1 to 28, about 1 to 26, about 1 to 24, about 1 to 22, about 1 to 20, about 1 to 18, about 1 to 16, about 1 to 14, about 1 to 12, about 1 to 10, about 1 to 8, about 1 to 6, about 1 one 4, about 1 to 2, about 4 to 36, about 4 to 34, about 4 to 32, about 4 to 30, about 4 to 28, about 4 to 26, about 4 to 24, about 4 to 22, about 4 to 20, about 4 to 18, about 4 to 16, about 4 to 14, about 4 to 12, about 4 to 10, about 4 to 8, about 4 to 6, about 6 to 36, about 6 to 34, about 6 to 32, about 6 to 30, about 6 to 28, about 6 to 26, about 6 to 24, about 6 to 22, about 6 to 20, about 6 to 18, about 6 to 16, about 6 to 14, about 6 to 12, about 6 to 10, about 6 to 8, about 8 to 36, about 8 to 34, about 8 to 32, about 8 to 30, about 8 to 28, about 8 to 26, about 8 to 24, about 8 to 22, about 8 to 20, about 8 to 18, about 8 to 16, about 8 to 14, about 8 to 12, about 8 to 10, about 10 to 36, about 10 to 34, about 10 to 32, about 10 to 30, about 10 to 28, about 10 to 26, about 10 to 24, about 10 to 22, about 10 to20, about 10 to 18, about 10 to 16, about 10 to 14, about 10 to 12, about 12 to 36, about 12 to34, about 12 to 32, about 12 to 30, about 12 to 28, about 12 to 26, about 12 to 24, about 12 to22, about 12 to 20, about 12 to 18, about 12 to 16, about 12 to 14, about 14 to 36, about 14 to34, about 14 to 32, about 14 to 30, about 14 to 28, about 14 to 26, about 14 to 24, about 14 to22, about 14 to 20, about 14 to 18, about 14 to 16, about 16 to 36, about 16 to 34, about 16 to32, about 16 to 30, about 16 to 28, about 16 to 26, about 16 to 24, about 16 to 22, about 16 to20, about 16 to 18, about 18 to 36, about 18 to 34, about 18 to 32, about 18 to 30, about 18 to28, about 18 to 26, about 18 to 24, about 18 to 22, about 18 to 20, about 20 to 36, about 20 to34, about 20 to 32, about 20 to 30, about 20 to 28, about 20 to 26, about 20 to 24, about 20 to22, about 22 to 36, about 22 to 34, about 22 to 32, about 22 to 30, about 22 to 28, about 22 to26, about 22 to 24, about 24 to 36, about 24 to 34, about 24 to 32, about 24 to 30, about 24 to28, about 24 to 26, about 26 to 36, about 26 to 34, about 26 to 32, about 26 to 30, about 26 to28, about 28 to 36, about 28 to 34, about 28 to 32, about 28 to 30, about 30 to 36, about 30 to34, about 30 to 32, about 32 to 36, about 32 to 34, or about 34 to 36 years.

[0201] In some embodiments, the microbial compositions of the present disclosure are shelf stable at refrigeration temperatures (35-40°F), at room temperature (68-72°F), between 50-77°F, between -23-35°F, between 70-100°F, or between 101-213°F for a period of 1 to 36, 1 to 34, 1 to 32, 1 to 30, 1 to 28, 1 to 26, 1 to 24, 1 to 22, 1 to 20, 1 to 18, 1 to 16, 1 to 14, 1 to 12, 1 to 10, 1 to 8, 1 to 6, 1 to 4, 1 to 2, 4 to 36, 4 to 34, 4 to 32, 4 to 30, 4 to 28, 4 to 26, 4 to 24, 4 to 22, 4 to 20, 4 to 18, 4 to 16, 4 to 14, 4 to 12, 4 to 10, 4 to 8, 4 to 6, 6 to 36, 6 to 34, 6 to 32, 6 to 30, 6 to 28, 6 to 26, 6 to 24, 6 to 22, 6 to 20, 6 to 18, 6 to 16, 6 to 14, 6 to 12, 6 to 10, 6 to 8, 8 to 36, 8 to 34, 8 to 32, 8 to 30, 8 to 28, 8 to 26, 8 to 24, 8 to 22, 8 to 20, 8 to 18, 8 to 16, 8 to 14, 8 to 12, 8 to 10, 10 to 36, 10 to 34, 10 to 32, 10 to 30, 10 to 28, 10 to 26, 10 to 24, 10 to 22, 10 to 20, 10 to 18, 10 to 16, 10 to 14, 10 to 12, 12 to 36, 12 to 34, 12 to 32, 12 to30, 12 to 28, 12 to 26, 12 to 24, 12 to 22, 12 to 20, 12 to 18, 12 to 16, 12 to 14, 14 to 36, 14 to34, 14 to 32, 14 to 30, 14 to 28, 14 to 26, 14 to 24, 14 to 22, 14 to 20, 14 to 18, 14 to 16, 16 to36, 16 to 34, 16 to 32, 16 to 30, 16 to 28, 16 to 26, 16 to 24, 16 to 22, 16 to 20, 16 to 18, 18 to36, 18 to 34, 18 to 32, 18 to 30, 18 to 28, 18 to 26, 18 to 24, 18 to 22, 18 to 20, 20 to 36, 20 to34, 20 to 32, 20 to 30, 20 to 28, 20 to 26, 20 to 24, 20 to 22, 22 to 36, 22 to 34, 22 to 32, 22 to30, 22 to 28, 22 to 26, 22 to 24, 24 to 36, 24 to 34, 24 to 32, 24 to 30, 24 to 28, 24 to 26, 26 to36, 26 to 34, 26 to 32, 26 to 30, 26 to 28, 28 to 36, 28 to 34, 28 to 32, 28 to 30, 30 to 36, 30 to34, 30 to 32, 32 to 36, 32 to 34, or 34 to 36 years.

[0202] In some embodiments, the microbial compositions of the present disclosure are shelf stable at any of the disclosed temperatures and / or temperature ranges and spans of time at a relative humidity of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, or 98%. In some embodiments, the microbial compositions of the present disclosure are shelf stable at any of the disclosed temperatures and / or temperature ranges and spans of time at a relative humidity of about l%-98%, about 5%-95%, about 1%-10%, about 5%-20%, about 15%-30%, about 20%-50%, about 40%-80%, about 50%-70%, about 40%-80%, about 60%-90%, about 70%-80%, about 70%-98%, or about 90%-98%.

[0203] In some embodiments, the microbial composition of the present disclosure possesses a water activity (aw) of less than 0.750, 0.700, 0.650, 0.600, 0.550, 0.500, 0.475, 0.450, 0.425, 0.400, 0.375, 0.350, 0.325, 0.300, 0.275, 0.250, 0.225, 0.200, 0.190, 0.180, 0.170, 0.160, 0.150, 0.140, 0.130, 0.120, 0.110, 0.100, 0.095, 0.090, 0.085, 0.080, 0.075, 0.070, 0.065, 0.060, 0.055, 0.050, 0.045, 0.040, 0.035, 0.030, 0.025, 0.020, 0.015, 0.010, or 0.005. In some embodiments, the microbial composition of the present disclosure possesses a water activity (aw) about 0.005 to about 0.750, about 0.010 to about 0.700, about 0.015 to about 0.550, about 0.020 to about 0.500, about 0.100 to about 0.400, about 0.005 to about 0.030, about 0.500 to about 0.750, about 0.080 to about 0.800, or about 0.040 to about 0.700.

[0204] In some embodiments, the microbial composition of the present disclosure possesses a water activity (aw) of less than about 0.750, about 0.700, about 0.650, about 0.600, about 0.550, about 0.500, about 0.475, about 0.450, about 0.425, about 0.400, about 0.375, about 0.350, about 0.325, about 0.300, about 0.275, about 0.250, about 0.225, about 0.200, about 0.190, about 0.180, about 0.170, about 0.160, about 0.150, about 0.140, about 0.130, about 0.120, about 0.110, about 0.100, about 0.095, about 0.090, about 0.085, about 0.080, about 0.075, about 0.070, about 0.065, about 0.060, about 0.055, about 0.050, about 0.045, about 0.040, about 0.035, about 0.030, about 0.025, about 0.020, about 0.015, about 0.010, or about 0.005.

[0205] The water activity values are determined by the method of Saturated Aqueous Solutions (Multon, “Techniques d’ Analyse E De Controle Dans Les Industries Agroalimentaires” APRIA (1981)) or by direct measurement using a viable Robotronic BT hygrometer or other hygrometer or hygroscope.

[0206] In some embodiments, the microbial composition comprises at least two different microbes, and wherein the at least two microbes are present in the composition at a ratio of 1 :2, 1 :3, 1 :3, 1 :5, 1 :6, 1 :7, 1 :8, 1 :9, 1 : 10, 1: 11, 1 : 12, 1 : 13, 1 : 14, 1 : 15, 1 : 16, 1 : 17, 1 : 18, 1 : 19, 1 :20, 1 :21, 1 :22, 1 :23, 1 :24, 1 :25, 1 :26, 1 :27, 1 :28, 1 :29, 1 :30, 1 :40, 1 :50, 1 :60, 1 : 100,1 : 125, 1 :150, 1 : 175, or 1 :200 or the inverse thereof. In some embodiments, the microbial composition comprises at least three different microbes, and wherein the three microbes are present in the composition at a ratio of 1 :2: 1, 1 : 1 :2, 2:2: 1, 1 :3: 1, 1 : 1 :3, 3: 1 :1, 3:3: 1, 1 :5: 1, 1 : 1 :5, 5: 1 : 1, 5:5: 1, or 1 :5:5.Encapsulating Compositions

[0207] In some embodiments, any one of the microbial signalers, microbial compositions of the disclosure are encapsulated in an encapsulating composition. An encapsulating composition protects the microbes from external stressors. In some embodiments, external stressors include thermal and physical stressors. In some embodiments, external stressors include chemicals present in the compositions. Encapsulating compositions further create an environment that may be beneficial to the microbes, such as minimizing the oxidative stresses of an aerobic environment on anaerobic microbes. See Kalsta et al. (US 5,104,662A), Ford (US 5,733,568A), and Mosbach and Nilsson (US 4,647,536A) for encapsulation compositions of microbes, and methods of encapsulating microbes.

[0208] In one embodiment, any one of the microbes, or microbial compositions of the present disclosure exhibits a thermal tolerance, which is used interchangeably with heat tolerance and heat resistance. In one embodiment, thermal tolerant compositions of the present disclosure are resistant to heat-killing and denaturation of the cell wall components and the intracellular environment.

[0209] In one embodiment, any one of the microbes, or microbial compositions of the present disclosure exhibits a pH tolerance, which is used interchangeably with acid tolerance and base tolerance. In one embodiment, pH tolerant compositions of the present disclosure are tolerant of the rapid swings in pH (high to low, low to high, high to neutral, low to neutral, neutral to high, and neutral to low) associated with one or more steps of preparing the composition. In some embodiments, any one of the microbes, or microbial compositions of the present disclosure exhibits a pH tolerance above at least pH 7, 8, 9, 10, 11, or 12. In some embodiments, any one of the microbes, or microbial compositions of the present disclosure exhibits a pH tolerance below at least pH 7, 6, 5, 4, 3, 2, or 1.

[0210] In one embodiment, the encapsulation is a reservoir-type encapsulation. In one embodiment, the encapsulation is a matrix-type encapsulation. In one embodiment, the encapsulation is a coated matrix-type encapsulation. Burgain et al. (2011. J. Food Eng. 104:467-483) discloses numerous encapsulation embodiments and techniques.

[0211] In some embodiments, the microbes, microbial compositions of the present disclosure are encapsulated in one or more of the following: gellan gum, xanthan gum, K- Carrageenan, cellulose acetate phthalate, chitosan, starch, milk fat, whey protein, Ca-alginate, raftilose, raftiline, pectin, saccharide, glucose, maltodextrin, gum arabic, guar, seed flour, alginate, dextrins, dextrans, celluloase, gelatin, gelatin, albumin, casein, gluten, acacia gum, tragacanth, wax, paraffin, stearic acid, monodiglycerides, and diglycerides. In some embodiments, the compositions of the present disclosure are encapsulated by one or more of a polymer, carbohydrate, sugar, plastic, glass, polysaccharide, lipid, wax, oil, fatty acid, or glyceride. In one embodiment, the microbial composition is encapsulated by glucose. In one embodiment, the microbial composition is encapsulated by a glucose-containing composition. In one embodiment, formulations of the microbial composition comprise a glucose encapsulant. In one embodiment, formulations of the microbial composition comprise a glucose-encapsulated composition.

[0212] In some embodiments, the encapsulation of the microbes, or microbial compositions of the present disclosure is carried out by an extrusion, emulsification, coating, agglomeration, lyophilization, vitrification, foam drying, preservation by vaporization, vacuum-drying, or spray-drying.

[0213] In some embodiments, the encapsulated compositions of the present disclosure are vitrified. In some embodiments, encapsulation involves a process of drying a composition of the present disclosure in the presence of a substance which forms a glassy, amorphous solid state, a process known as vitrification, and in doing so encapsulates the composition. In some embodiments, the vitrified composition is protected from degradative conditions that would typically destroy or degrade microbes. Many common substances have the property of vitrification; that is, they will form a glassy solid state under certain conditions. Among these substances are several sugars, including sucrose and maltose, and other more complex compounds, such as polyvinylpyrrolidone (PVP). As any solution dries down, the molecules in the solution can either crystalize, or they can vitrify. A solute which has an extensive asymmetry may be a superior vitrifier, because of the hindrances to nucleation of crystals during drying. A substance that inhibits the crystallization of another substance may result in the combined substances forming a superior vitrification, such as raffinose in the presence of sucrose. See U.S. Patent Nos. 5,290,765 and 9,469,835.

[0214] In some embodiments, a microbial composition is produced that is encapsulated in a vitrified substance. The vitrified composition may be created by selecting a mixtureincluding cells; combining said mixture with sufficient quantity of one or more vitrifying solutes to protect said mixture during drying and to inhibit destructive reactions; and drying said combination by exposing said combination to a desiccant, or desiccating conditions, at a temperature above that which said combination will freeze and below that at which said vitrifying solutes achieve the vitrified state, at approximately normal atmospheric pressure, until said combination is substantially dry.

[0215] In one embodiment, the encapsulating composition comprises microcapsules having a multiplicity of liquid cores encapsulated in a solid shell material. For purposes of the disclosure, a “multiplicity” of cores is defined as two or more.

[0216] One category of fusible materials useful as encapsulating shell materials is that of waxes. Representative waxes contemplated for use herein are as follows: animal waxes, such as beeswax, lanolin, shell wax, and Chinese insect wax; vegetable waxes, such as carnauba, candelilla, bayberry, and sugar cane; mineral waxes, such as paraffin, microcrystalline petroleum, ozocerite, ceresin, and montan; synthetic waxes, such as low molecular weight polyolefin (e.g., CARBOWAX), and polyol ether-esters (e.g., sorbitol); Fischer-Tropsch process synthetic waxes; and mixtures thereof. Water-soluble waxes, such as CARBOWAX and sorbitol, are not contemplated herein if the core is aqueous. Still other fusible compounds useful herein are fusible natural resins, such as rosin, balsam, shellac, and mixtures thereof.

[0217] In some embodiments, the microbes, or microbial compositions of the present disclosure is embedded in a wax, such as the waxes described in the present disclosure. In some embodiments, the microbes or microbial composition is embedded in wax balls. In some embodiments, the microbes or microbial composition is already encapsulated prior to being embedded in wax balls. In some embodiments, the wax balls are 10 microns, 20 microns, 30 microns, 40 microns, 50 microns, 60 microns, 70 microns, 80 microns, 90 microns, 100 microns, 150 microns, 200 microns, 250 microns, 300 microns, 350 microns, 400 microns, 450 microns, 500 microns, 550 microns, 600 microns, 650 microns, 700 microns, 750 microns, 800 microns, 850 microns, 900 microns, 950 microns, or 1,000 microns in diameter.

[0218] In some embodiments, the wax balls are about 10 microbes, about 20 microns, about 30 microns, about 40 microns, about 50 microns, about 60 microns, about 70 microns, about 80 microns, about 90 microns, about 100 microns, about 150 microns, about 200 microns, about 250 microns, about 300 microns, about 350 microns, about 400 microns, about 450 microns, about 500 microns, about 550 microns, about 600 microns, about 650 microns, about700 microns, about 750 microns, about 800 microns, about 850 microns, about 900 microns, about 950 microns, or about 1,000 microns in diameter.

[0219] In some embodiments, the wax balls are between 10-20 microns, 10-30 microns,10-40 microns, 10-50 microns, 10-60 microns, 10-70 microns, 10-80 microns, 10-90 microns, 10-100 microns, 10-250 microns, 10-500 microns, 10-750 microns, 10-1,000 microns, 20-30 microns, 20-40 microns, 20-50 microns, 20-60 microns, 20-70 microns, 20-80 microns, 20-90 microns, 20-100 microns, 20-250 microns, 20-500 microns, 20-750 microns, 20-1,000 microns, 30-40 microns, 30-50 microns, 30-60 microns, 30-70 microns, 30-80 microns, 30-90 microns, 30-100 microns, 30-250 microns, 30-500 microns, 30-750 microns, 30-1,000 microns, 40-50 microns, 40-60 microns, 40-70 microns, 40-80 microns, 40-90 microns, 40-100 microns, 40-250 microns, 40-500 microns, 40-750 microns, 40-1,000 microns, 50-60 microns, 50-70 microns, 50-80 microns, 50-90 microns, 50-100 microns, 50-250 microns, 50-500 microns, 50-750 microns, 50-1,000 microns, 60-70 microns, 60-80 microns, 60-90 microns, 60-100 microns, 60-250 microns, 60-500 microns, 60-750 microns, 60-1,000 microns, 70-80 microns 70-90 microns, 70-90 microns, 70-100 microns, 70-250 microns, 70-500 microns, 70- 750 microns, 70-1,000 microns, 80-90 microns, 80-100 microns, 80-250 microns, 80-500 microns, 80-500 microns, 80-750 microns, 80-1,000 microns, 90-100 microns, 90-250 microns, 90-500 microns, 90-750 microns, 90-1,000 microns, 100-250 microns, 100-500 microns, 100-750 microns, 100-1,000 microns, 250-500 microns, 250-750 microns, 250-1,000 microns, 500-750 microns, 500-1,000 microns, or 750-1,000 microns in diameter.

[0220] In some embodiments, the wax balls are between about 10-20 microns, about 10-30 microns, about 10-40 microns, about 10-50 microns, about 10-60 microns, about 10-70 microns, about 10-80 microns, about 10-90 microns, about 10-100 microns, about 10-250 microns, about 10-500 microns, about 10-750 microns, about 10-1,000 microns, about 20-30 microns, about 20-40 microns, about 20-50 microns, about 20-60 microns, about 20-70 microns, about 20-80 microns, about 20-90 microns, about 20-100 microns, about 20-250 microns, about 20-500 microns, about 20-750 microns, about 20-1,000 microns, about 30-40 microns, about 30-50 microns, about 30-60 microns, about 30-70 microns, about 30-80 microns, about 30-90 microns, about 30-100 microns, about 30-250 microns, about 30-500 microns, about 30-750 microns, about 30-1,000 microns, about 40-50 microns, about 40-60 microns, about 40-70 microns, about 40-80 microns, about 40-90 microns, about 40-100 microns, about 40-250 microns, about 40-500 microns, about 40-750 microns, about 40-1,000 microns, about 50-60 microns, about 50-70 microns, about 50-80 microns, about 50-90microns, about 50-100 microns, about 50-250 microns, about 50-500 microns, about 50-750 microns, about 50-1,000 microns, about 60-70 microns, about 60-80 microns, about 60-90 microns, about 60-100 microns, about 60-250 microns, about 60-500 microns, about 60-750 microns, about 60-1,000 microns, about 70-80 microns about 70-90 microns, about 70-90 microns, about 70-100 microns, about 70-250 microns, about 70-500 microns, about 70-750 microns, about 70-1,000 microns, about 80-90 microns, about 80-100 microns, about 80-250 microns, about 80-500 microns, about 80-500 microns, about 80-750 microns, about 80-1,000 microns, about 90-100 microns, about 90-250 microns, about 90-500 microns, about 90-750 microns, about 90-1,000 microns, about 100-250 microns, about 100-500 microns, about 100- 750 microns, about 100-1,000 microns, about 250-500 microns, about 250-750 microns, about 250-1,000 microns, about 500-750 microns, about 500-1,000 microns, or about 750-1,000 microns in diameter.

[0221] Various adjunct materials are contemplated for incorporation in fusible materials according to the present disclosure. For example, antioxidants, light stabilizers, dyes and lakes, flavors, essential oils, anti-caking agents, fillers, Ph stabilizers, sugars (monosaccharides, disaccharides, trisaccharides, and polysaccharides) and the like can be incorporated in the fusible material in amounts which do not diminish its utility for the present disclosure.

[0222] The core material contemplated herein constitutes from about 0.1% to about 50%, about 1% to about 35%, or about 5% to about 30% by weight of the microcapsules. In some embodiments, the core material contemplated herein constitutes no more than about 30% by weight of the microcapsules. In some embodiments, the core material contemplated herein constitutes about 5% by weight of the microcapsules. The core material is contemplated as either a liquid or solid at contemplated storage temperatures of the microcapsules.

[0223] The cores may include other additives well-known in the agricultural art, including other potentially useful supplemental core materials will be apparent to those of ordinary skill in the art. Emulsifying agents may be employed to assist in the formation of stable emulsions. Representative emulsifying agents include glyceryl monostearate, polysorbate esters, ethoxylated mono- and diglycerides, and mixtures thereof.

[0224] For ease of processing, and particularly to enable the successful formation of a reasonably stable emulsion, the viscosities of the core material and the shell material should be similar at the temperature at which the emulsion is formed. In particular, the ratio of theviscosity of the shell to the viscosity of the core, expressed in centipoise or comparable units, and both measured at the temperature of the emulsion, should be from about 22: 1 to about 1 : 1, desirably from about 8: 1 to about 1 : 1, and preferably from about 3: 1 to about 1 : 1. A ratio of 1 : 1 would be ideal, but a viscosity ratio within the recited ranges is useful.

[0225] Encapsulating compositions are not limited to microcapsule compositions as disclosed above. In some embodiments encapsulating compositions encapsulate the microbial compositions in an adhesive polymer that can be natural or synthetic without toxic effect. In some embodiments, the encapsulating composition may be a matrix selected from sugar matrix, gelatin matrix, polymer matrix, silica matrix, starch matrix, foam matrix, glass / glassy matrix etc. See Pirzio et al. (U.S. Patent 7,488,503). In some embodiments, the encapsulating composition may be selected from polyvinyl acetates; polyvinyl acetate copolymers; ethylene vinyl acetate (EVA) copolymers; polyvinyl alcohols; polyvinyl alcohol copolymers; celluloses, including ethylcelluloses, methylcelluloses, hydroxymethylcelluloses, hydroxypropylcelluloses and carboxymethylcellulose; polyvinylpyrolidones; polysaccharides, including starch, modified starch, dextrins, maltodextrins, alginate and chitosans; monosaccharides; fats; fatty acids, including oils; proteins, including gelatin and zeins; gum arabics; shellacs; vinylidene chloride and vinylidene chloride copolymers; calcium lignosulfonates; acrylic copolymers; polyvinylacrylates; polyethylene oxide; acrylamide polymers and copolymers; polyhydroxyethyl acrylate, methylacrylamide monomers; and polychloroprene.

[0226] In some embodiments, the encapsulating compositions comprise at least one layer of encapsulation. In some embodiments, the encapsulating compositions comprise at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, or at least 20 layers of encapsulation / encapsulants. In some embodiments, the encapsulating compositions comprise about 1 to about 20 layers, about 2 to about 20 layers, about 2 to about 5 layers, about 1 to about 5 layers, about 10 to about 20 layers, about 15 to about 20 layers, about 5 to about 10 layers, about 5 to about 15 layers, or about 2 to about 7 layers of encapsulation / encapsulants.

[0227] In some embodiments, the encapsulating compositions comprise at least two layers of encapsulation. In some embodiments, each layer of encapsulation confers a different characteristic to the composition. In some embodiments, no two consecutive layers confer the same characteristic. In some embodiments, at least one layer of the at least two layers ofencapsulation confers thermostability, shelf stability, ultraviolet resistance, moisture resistance, hydrophobicity, hydrophilicity, lipophobicity, lipophilicity, pH stability, acid resistance, and base resistance.

[0228] In some embodiments, the encapsulating compositions comprise two layers of encapsulation; the first layer confers thermostability and / or shelf stability, and the second layer provides pH resistance. In some embodiments, the encapsulating layers confer a timed release of the microbial composition held in the center of the encapsulating layers. In some embodiments, the greater the number of layers confers a greater amount of time before the microbial composition is exposed, post administration.

[0229] In some embodiments, the encapsulating shell of the present disclosure can be up to 10pm, 20pm, 30pm, 40pm, 50pm, 60pm, 70pm, 80pm, 90pm, 100pm, 110pm, 120pm, 130pm, 140pm, 150pm, 160pm, 170pm, 180pm, 190pm, 200pm, 210pm, 220pm, 230pm,240pm, 250pm, 260pm, 270pm, 280pm, 290pm, 300pm, 310pm, 320pm, 330pm, 340pm,350pm, 360pm, 370pm, 380pm, 390pm, 400pm, 410pm, 420pm, 430pm, 440pm, 450pm, 460pm, 470pm, 480pm, 490pm, 500pm, 510pm, 520pm, 530pm, 540pm, 550pm, 560pm,570pm, 580pm, 590pm, 600pm, 610pm, 620pm, 630pm, 640pm, 650pm, 660pm, 670pm,680pm, 690pm, 700pm, 710pm, 720pm, 730pm, 740pm, 750pm, 760pm, 770pm, 780pm,790pm, 800pm, 810pm, 820pm, 830pm, 840pm, 850pm, 860pm, 870pm, 880pm, 890pm,900pm, 910pm, 920pm, 930pm, 940pm, 950pm, 960pm, 970pm, 980pm, 990pm, 1000pm, 1010pm, 1020pm, 1030pm, 1040pm, 1050pm, 1060pm, 1070pm, 1080pm, 1090pm,1100pm, 1110pm, 1120pm, 1130pm, 1140pm, 1150pm, 1160pm, 1170pm, 1180pm,1190pm, 1200pm, 1210pm, 1220pm, 1230pm, 1240pm, 1250pm, 1260pm, 1270pm,1280pm, 1290pm, 1300pm, 1310pm, 1320pm, 1330pm, 1340pm, 1350pm, 1360pm,1370pm, 1380pm, 1390pm, 1400pm, 1410pm, 1420pm, 1430pm, 1440pm, 1450pm, 1460pm, 1470pm, 1480pm, 1490pm, 1500pm, 1510pm, 1520pm, 1530pm, 1540pm,1550pm, 1560pm, 1570pm, 1580pm, 1590pm, 1600pm, 1610pm, 1620pm, 1630pm,1640pm, 1650pm, 1660pm, 1670pm, 1680pm, 1690pm, 1700pm, 1710pm, 1720pm,1730pm, 1740pm, 1750pm, 1760pm, 1770pm, 1780pm, 1790pm, 1800pm, 1810pm,1820pm, 1830pm, 1840pm, 1850pm, 1860pm, 1870pm, 1880pm, 1890pm, 1900pm, 1910pm, 1920pm, 1930pm, 1940pm, 1950pm, 1960pm, 1970pm, 1980pm, 1990pm,2000pm, 2010pm, 2020pm, 2030pm, 2040pm, 2050pm, 2060pm, 2070pm, 2080pm,2090pm, 2100pm, 2110pm, 2120pm, 2130pm, 2140pm, 2150pm, 2160pm, 2170pm,2180pm, 2190pm, 2200pm, 2210pm, 2220pm, 2230pm, 2240pm, 2250pm, 2260pm,2270pm, 2280pm, 2290pm, 2300pm, 2310pm, 2320pm, 2330pm, 2340pm, 2350pm,2360pm, 2370pm, 2380pm, 2390pm, 2400pm, 2410pm, 2420pm, 2430pm, 2440pm,2450pm, 2460pm, 2470pm, 2480pm, 2490pm, 2500pm, 2510pm, 2520pm, 2530pm,2540pm, 2550pm, 2560pm, 2570pm, 2580pm, 2590pm, 2600pm, 2610pm, 2620pm,2630pm, 2640pm, 2650pm, 2660pm, 2670pm, 2680pm, 2690pm, 2700pm, 2710pm,2720pm, 2730pm, 2740pm, 2750pm, 2760pm, 2770pm, 2780pm, 2790pm, 2800pm,2810pm, 2820pm, 2830pm, 2840pm, 2850pm, 2860pm, 2870pm, 2880pm, 2890pm,2900pm, 2910pm, 2920pm, 2930pm, 2940pm, 2950pm, 2960pm, 2970pm, 2980pm,2990pm, or 3000pm thick. In some embodiments, the encapsulating shell of the present disclosure is about 10 pm to about 1000 pm, about 20pm to about 200 pm, about 20 pm to about 1000 pm, about 100 pm to about 1000 pm, about 100 pm to about 500 pm, about 10 pm to about 50 pm, about 300 pm to about 500 pm, about 2000 pm to about 3000 pm, about 1000 pm to about 2500 pm, or about 2500 pm to about 3000 pm thick.

[0230] In some embodiments, the encapsulation composition of the present disclosure possesses a water activity (aw) of less than 0.750, 0.700, 0.650, 0.600, 0.550, 0.500, 0.475, 0.450, 0.425, 0.400, 0.375, 0.350, 0.325, 0.300, 0.275, 0.250, 0.225, 0.200, 0.190, 0.180, 0.170, 0.160, 0.150, 0.140, 0.130, 0.120, 0.110, 0.100, 0.095, 0.090, 0.085, 0.080, 0.075, 0.070, 0.065, 0.060, 0.055, 0.050, 0.045, 0.040, 0.035, 0.030, 0.025, 0.020, 0.015, 0.010, or 0.005. In some embodiments, the encapsulation composition of the present disclosure possesses a water activity (aw) about 0.005 to about 0.750, about 0.010 to about 0.700, about 0.015 to about 0.550, about 0.020 to about 0.500, about 0.100 to about 0.400, about 0.005 to about 0.030, about 0.500 to about 0.750, about 0.080 to about 0.800, or about 0.040 to about 0.700.

[0231] In some embodiments, the encapsulation composition of the present disclosure possesses a water activity (aw) of less than about 0.750, about 0.700, about 0.650, about 0.600, about 0.550, about 0.500, about 0.475, about 0.450, about 0.425, about 0.400, about 0.375, about 0.350, about 0.325, about 0.300, about 0.275, about 0.250, about 0.225, about 0.200, about 0.190, about 0.180, about 0.170, about 0.160, about 0.150, about 0.140, about 0.130, about 0.120, about 0.110, about 0.100, about 0.095, about 0.090, about 0.085, about 0.080, about 0.075, about 0.070, about 0.065, about 0.060, about 0.055, about 0.050, about 0.045, about 0.040, about 0.035, about 0.030, about 0.025, about 0.020, about 0.015, about 0.010, or about 0.005.

[0232] In one embodiment, the microbe(s) are first dried by spray dry, lyophilization, or foam drying along with excipients that may include one or more sugars, sugar alcohols,disaccharides, trisaccharides, polysaccharides, salts, amino acids, amino acid salts, or polymers.

[0233] In some embodiments, the microbes or compositions comprising the microbes are milled to a size of 10 microns, 20 microns, 30 microns, 40 microns, 50 microns, 60 microns, 70 microns, 80 microns, 90 microns, 100 microns, 150 microns, 200 microns, 250 microns, 300 microns, 350 microns, 400 microns, 450 microns, 500 microns, 550 microns, 600 microns, 650 microns, 700 microns, 750 microns, 800 microns, 850 microns, 900 microns, 950 microns, or 1,000 microns in diameter.

[0234] In some embodiments, the microbes or compositions comprising the microbes are milled to a size of about 10 microns, about 20 microns, about 30 microns, about 40 microns, about 50 microns, about 60 microns, about 70 microns, about 80 microns, about 90 microns, about 100 microns, about 150 microns, about 200 microns, about 250 microns, about 300 microns, about 350 microns, about 400 microns, about 450 microns, about 500 microns, about 550 microns, about 600 microns, about 650 microns, about 700 microns, about 750 microns, about 800 microns, about 850 microns, about 900 microns, about 950 microns, or about 1,000 microns in diameter.

[0235] In some embodiments, the microbes or compositions comprising the microbes are milled to a size of between 10-20 microns, 10-30 microns, 10-40 microns, 10-50 microns, 10-60 microns, 10-70 microns, 10-80 microns, 10-90 microns, 10-100 microns, 10-250 microns, 10-500 microns, 10-750 microns, 10-1,000 microns, 20-30 microns, 20-40 microns, 20-50 microns, 20-60 microns, 20-70 microns, 20-80 microns, 20-90 microns, 20-100 microns, 20-250 microns, 20-500 microns, 20-750 microns, 20-1,000 microns, 30-40 microns, 30-50 microns, 30-60 microns, 30-70 microns, 30-80 microns, 30-90 microns, 30-100 microns, 30- 250 microns, 30-500 microns, 30-750 microns, 30-1,000 microns, 40-50 microns, 40-60 microns, 40-70 microns, 40-80 microns, 40-90 microns, 40-100 microns, 40-250 microns, 40- 500 microns, 40-750 microns, 40-1,000 microns, 50-60 microns, 50-70 microns, 50-80 microns, 50-90 microns, 50-100 microns, 50-250 microns, 50-500 microns, 50-750 microns, 50-1,000 microns, 60-70 microns, 60-80 microns, 60-90 microns, 60-100 microns, 60-250 microns, 60-500 microns, 60-750 microns, 60-1,000 microns, 70-80 microns 70-90 microns, 70-90 microns, 70-100 microns, 70-250 microns, 70-500 microns, 70-750 microns, 70-1,000 microns, 80-90 microns, 80-100 microns, 80-250 microns, 80-500 microns, 80-500 microns, 80-750 microns, 80-1,000 microns, 90-100 microns, 90-250 microns, 90-500 microns, 90-750 microns, 90-1,000 microns, 100-250 microns, 100-500 microns, 100-750 microns, 100-1,000microns, 250-500 microns, 250-750 microns, 250-1,000 microns, 500-750 microns, 500-1,000 microns, or 750-1,000 microns in diameter.

[0236] In some embodiments, the microbes or compositions comprising the microbes are milled to a size of between about 10-20 microns, about 10-30 microns, about 10-40 microns, about 10-50 microns, about 10-60 microns, about 10-70 microns, about 10-80 microns, about 10-90 microns, about 10-100 microns, about 10-250 microns, about 10-500 microns, about 10-750 microns, about 10-1,000 microns, about 20-30 microns, about 20-40 microns, about 20-50 microns, about 20-60 microns, about 20-70 microns, about 20-80 microns, about 20-90 microns, about 20-100 microns, about 20-250 microns, about 20-500 microns, about 20-750 microns, about 20-1,000 microns, about 30-40 microns, about 30-50 microns, about 30-60 microns, about 30-70 microns, about 30-80 microns, about 30-90 microns, about 30-100 microns, about 30-250 microns, about 30-500 microns, about 30-750 microns, about 30-1,000 microns, about 40-50 microns, about 40-60 microns, about 40-70 microns, about 40-80 microns, about 40-90 microns, about 40-100 microns, about 40-250 microns, about 40-500 microns, about 40-750 microns, about 40-1,000 microns, about 50-60 microns, about 50-70 microns, about 50-80 microns, about 50-90 microns, about 50-100 microns, about 50-250 microns, about 50-500 microns, about 50-750 microns, about 50-1,000 microns, about 60-70 microns, about 60-80 microns, about 60-90 microns, about 60-100 microns, about 60-250 microns, about 60-500 microns, about 60-750 microns, about 60-1,000 microns, about 70-80 microns about 70-90 microns, about 70-90 microns, about 70-100 microns, about 70-250 microns, about 70-500 microns, about 70-750 microns, about 70-1,000 microns, about 80-90 microns, about 80-100 microns, about 80-250 microns, about 80-500 microns, about 80-500 microns, about 80-750 microns, about 80-1,000 microns, about 90-100 microns, about 90-250 microns, about 90-500 microns, about 90-750 microns, about 90-1,000 microns, about 100-250 microns, about 100-500 microns, about 100-750 microns, about 100- 1,000 microns, about 250-500 microns, about 250-750 microns, about 250-1,000 microns, about 500-750 microns, about 500-1,000 microns, or about 750-1,000 microns in diameter.

[0237] In some embodiments, the microbes or compositions comprising the microbes are combined with a wax, fat, oil, fatty acid, or fatty alcohol, and spray congealed into beads of about 10 microns, about 20 microns, about 30 microns, about 40 microns, about 50 microns, about 60 microns, about 70 microns, about 80 microns, about 90 microns, about 100 microns, about 150 microns, about 200 microns, about 250 microns, about 300 microns, about 350 microns, about 400 microns, about 450 microns, about 500 microns, about 550 microns, about600 microns, about 650 microns, about 700 microns, about 750 microns, about 800 microns, about 850 microns, about 900 microns, about 950 microns, or about 1,000 microns in diameter.

[0238] In some embodiments, the microbes or compositions comprising the microbes are combined with a wax, fat, oil, fatty acid, or fatty alcohol, and spray congealed into beads of between 10-20 microns, 10-30 microns, 10-40 microns, 10-50 microns, 10-60 microns, 10- 70 microns, 10-80 microns, 10-90 microns, 10-100 microns, 10-250 microns, 10-500 microns, 10-750 microns, 10-1,000 microns, 20-30 microns, 20-40 microns, 20-50 microns, 20-60 microns, 20-70 microns, 20-80 microns, 20-90 microns, 20-100 microns, 20-250 microns, 20- 500 microns, 20-750 microns, 20-1,000 microns, 30-40 microns, 30-50 microns, 30-60 microns, 30-70 microns, 30-80 microns, 30-90 microns, 30-100 microns, 30-250 microns, SO- SOO microns, 30-750 microns, 30-1,000 microns, 40-50 microns, 40-60 microns, 40-70 microns, 40-80 microns, 40-90 microns, 40-100 microns, 40-250 microns, 40-500 microns, 40- 750 microns, 40-1,000 microns, 50-60 microns, 50-70 microns, 50-80 microns, 50-90 microns, 50-100 microns, 50-250 microns, 50-500 microns, 50-750 microns, 50-1,000 microns, 60-70 microns, 60-80 microns, 60-90 microns, 60-100 microns, 60-250 microns, 60-500 microns, 60- 750 microns, 60-1,000 microns, 70-80 microns 70-90 microns, 70-90 microns, 70-100 microns, 70-250 microns, 70-500 microns, 70-750 microns, 70-1,000 microns, 80-90 microns, 80-100 microns, 80-250 microns, 80-500 microns, 80-500 microns, 80-750 microns, 80-1,000 microns, 90-100 microns, 90-250 microns, 90-500 microns, 90-750 microns, 90-1,000 microns, 100-250 microns, 100-500 microns, 100-750 microns, 100-1,000 microns, 250-500 microns, 250-750 microns, 250-1,000 microns, 500-750 microns, 500-1,000 microns, or 750- 1,000 microns in diameter.

[0239] In some embodiments, the microbes or compositions comprising the microbes are combined with a wax, fat, oil, fatty acid, or fatty alcohol, and spray congealed into beads of between about 10-20 microns, about 10-30 microns, about 10-40 microns, about 10-50 microns, about 10-60 microns, about 10-70 microns, about 10-80 microns, about 10-90 microns, about 10-100 microns, about 10-250 microns, about 10-500 microns, about 10-750 microns, about 10-1,000 microns, about 20-30 microns, about 20-40 microns, about 20-50 microns, about 20-60 microns, about 20-70 microns, about 20-80 microns, about 20-90 microns, about 20-100 microns, about 20-250 microns, about 20-500 microns, about 20-750 microns, about 20-1,000 microns, about 30-40 microns, about 30-50 microns, about 30-60 microns, about 30-70 microns, about 30-80 microns, about 30-90 microns, about 30-100 microns, about 30-250 microns, about 30-500 microns, about 30-750 microns, about 30-1,000microns, about 40-50 microns, about 40-60 microns, about 40-70 microns, about 40-80 microns, about 40-90 microns, about 40-100 microns, about 40-250 microns, about 40-500 microns, about 40-750 microns, about 40-1,000 microns, about 50-60 microns, about 50-70 microns, about 50-80 microns, about 50-90 microns, about 50-100 microns, about 50-250 microns, about 50-500 microns, about 50-750 microns, about 50-1,000 microns, about 60-70 microns, about 60-80 microns, about 60-90 microns, about 60-100 microns, about 60-250 microns, about 60-500 microns, about 60-750 microns, about 60-1,000 microns, about 70-80 microns about 70-90 microns, about 70-90 microns, about 70-100 microns, about 70-250 microns, about 70-500 microns, about 70-750 microns, about 70-1,000 microns, about 80-90 microns, about 80-100 microns, about 80-250 microns, about 80-500 microns, about 80-500 microns, about 80-750 microns, about 80-1,000 microns, about 90-100 microns, about 90-250 microns, about 90-500 microns, about 90-750 microns, about 90-1,000 microns, about 100-250 microns, about 100-500 microns, about 100-750 microns, about 100-1,000 microns, about 250- 500 microns, about 250-750 microns, about 250-1,000 microns, about 500-750 microns, about 500-1,000 microns, or about 750-1,000 microns in diameter.

[0240] In some embodiments, the microbes or compositions comprising the microbes are combined with a wax, fat, oil, fatty acid, or fatty alcohol as well as a water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol and spray congealed into beads, the size of which are described herein. In some embodiments, the water-soluble polymer, salt, polysaccharide, sugar, or sugar alcohol serves as a disintegrant. In some embodiments, the disintegrant forms pores once the beads are dispersed in the soil.

[0241] In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves within 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, or 60 minutes of being administered. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves within about 1, about 5, about 10, about 15, about 20, about 25, about 30, about 35, about 40, about 45, about 50, about 55, or about 60 minutes of being administered. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves within about 1-10 minutes, 2-10 minutes, 1-5 minutes, 10-20 minutes, 15-20 minutes, 30-45 minutes, 1-60 minutes, 20-60 minutes, 30-60 minutes or about 50-60 minutes of being administered.

[0242] In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves within 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10,10.5, 11, 11.5, or 12 hours of being administered. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves within about 1, about 1.5, about 2, about 2.5, about 3, about 3.5, about 4, about 4.5, about 5, about 5.5, about 6, about 6.5, about 7, about7.5, about 8, about 8.5, about 9, about 9.5, about 10, about 10.5, about 11, about 11.5, or about 12 hours of being administered. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves within 1-2 hours, 1-5 hours, 2-6 hours, 10-12 hours, 5-12 hours, 5-10 hours, 3-9 hours, or about 9-12 hours of being administered.

[0243] In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves at a temperature of at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 °C. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves at a temperature of at least about 10, least about 11, least about 12, least about 13, least about 14, least about 15, least about 16, least about 17, least about 18, least about 19, least about 20, least about 21, least about 22, least about 23, least about 24, least about 25, least about 26, least about 27, least about 28, least about 29, least about 30, least about 31, least about 32, least about 33, least about 34, about 35, about 36, about 37, about 38, about 39, about 40, about 41, about 42, about 43, about 44, least about 45, least about 46, least about 47, least about 48, least about 49, or least about 50 °C. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves at a temperature of about 10-20 °C, about 10-25 °C, about 10-30 °C, about 20-40 °C, about 40-509 °C, about 45-50 °C, about 30-50 °C, about 20-25 °C, or about 20-35 °C.

[0244] In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves at a pH of at least 3.8, 3.9, 4. 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2,7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3,9.4, 9.5, 9.6, 9.7, 9.8, 9.9 or 10.0. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves at a pH of at least about 3.8, least about 3.9, least about 4, least about 4.1, least about 4.2, least about 4.3, least about 4.4, least about 4.5, least about 4.6, least about 4.7, least about 4.8, least about 4.9, least about 5.0, least about 5.1, least about 5.2, least about 5.3, least about 5.4, least about 5.5, least about 5.6, least about 5.7, least about 5.8, least about 5.9, least about 6.0, least about 6.2, least about 6.3, least about 6.4, least about 6.5, least about 6.6, least about 6.7, least about 6.8, least about 6.9, least about 7.0, least about 7.1, least about 7.2, least about 7.3, least about 7.4, least about 7.5, least about 7.6, least about 7.7, least about 7.8, least about 7.9, least about 8.0, least about 8.1, least about 8.2, least about 8.3, least about 8.4, least about 8.5, least about 8.6, least about 8.7, least about 8.8, least about 8.9, least about 9.0, least about 9.1, least about 9.2, least about 9.3, least about 9.4, least about 9.5, least about 9.6, least about 9.7, least about 9.8, least about 9.9, or least about 10.0. In some embodiments, the composition of the water-soluble polymer, salt, polysaccharide, sugar, polypeptide, protein, or sugar alcohol is modified such that the disintegrant dissolves at a pH of about 3-6, about 3-5, about 3-4, about 4-5, about 4-6, about 3-7, about 4-7, about 5-7, about 6-7, about 6-8, about 7-8, about 7-9, about 8-10, about 9-10, about 9-11, or about 8-11.

[0245] In some embodiments, the microbes or compositions comprising the microbes are coated with a polymer, a polysaccharide, sugar, sugar alcohol, gel, wax, fat, fatty alcohol, or fatty acid

[0246] In some embodiments, the microbes or compositions comprising the microbes are coated with a polymer, a polysaccharide, sugar, sugar alcohol, gel, wax, fat, fatty alcohol, or fatty acid.

[0247] In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves within 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, or 60 minutes of being administered. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves within about 1, about 5, about 10, about 15, about 20, about 25, about 30, about 35, about 40, about 45, about 50, about 55, or about 60 minutes of being administered. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves within about 1-60, about 1-10, about 10-15, about 2-10, about 5-20, about 5- 30, about 30-60, about 45-60, or about 5-60 minutes of being administered.

[0248] In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves within 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5,5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, or 12 hours of being administered. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves within about 1, about 1.5, about 2, about 2.5, about 3, about 3.5, about 4, about 4.5, about 5, about 5.5, about 6, about 6.5, about 7, about 7.5, about 8, about8.5, about 9, about 9.5, about 10, about 10.5, about 11, about 11.5, or about 12 hours of being administered. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves within about 1-2 hours, about 1-3 hours, about 1-5 hours, about 3-6 hours, about 5-10 hours, about 5-12 hours, about 6-12 hours, about 8-12 hours, or about 10-12 hours of being administered.

[0249] In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves at a temperature of at least 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 °C. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves at a temperature of at least about 10, least about 11, least about 12, least about 13, least about14, least about 15, least about 16, least about 17, least about 18, least about 19, least about 20, least about 21, least about 22, least about 23, least about 24, least about 25, least about 26, least about 27, least about 28, least about 29, least about 30, least about 31, least about 32, least about 33, least about 34, about 35, about 36, about 37, about 38, about 39, about 40, about 41, about 42, about 43, about 44, least about 45, least about 46, least about 47, least about 48, least about 49, or least about 50 °C. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves at a temperature of about 10-20 °C, about 15-20 °C, about 10-25 °C, about 20-30 °C, about 40-50 °C, about 35-45 °C, about 45-50 °C, or about 30-50 °C.

[0250] In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves at a pH of at least 3.8, 3.9, 4. 4.1, 4.2,4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.2, 6.3, 6.4,6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5,8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9 or 10.0. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves at a pH of at least about 3.8, least about 3.9, least about 4, least about 4.1,least about 4.2, least about 4.3, least about 4.4, least about 4.5, least about 4.6, least about 4.7, least about 4.8, least about 4.9, least about 5.0, least about 5.1, least about 5.2, least about 5.3, least about 5.4, least about 5.5, least about 5.6, least about 5.7, least about 5.8, least about 5.9, least about 6.0, least about 6.2, least about 6.3, least about 6.4, least about 6.5, least about 6.6, least about 6.7, least about 6.8, least about 6.9, least about 7.0, least about 7.1, least about 7.2, least about 7.3, least about 7.4, least about 7.5, least about 7.6, least about 7.7, least about 7.8, least about 7.9, least about 8.0, least about 8.1, least about 8.2, least about 8.3, least about 8.4, least about 8.5, least about 8.6, least about 8.7, least about 8.8, least about 8.9, least about 9.0, least about 9.1, least about 9.2, least about 9.3, least about 9.4, least about 9.5, least about 9.6, least about 9.7, least about 9.8, least about 9.9, or least about 10.0. In some embodiments, the coating of the microbes or compositions comprising the microbes is modified such that the coating dissolves at a pH of about 3-4, about 3-5, about 4-5, about 5-6, about 5-7, about 4-7, about 6-7, about 6-8, about 7-8, about 7-9, about 8-10, about 8-9, about 8-11, about 9-11, or about 10-11.Agricultural Applications of Microbial Compositions

[0251] The microbial compositions disclosed herein may be in the form of a dry powder, a slurry of powder and water, a granular material, or a flowable seed treatment. The compositions comprising microbe populations disclosed herein may be coated on a surface of a seed, and may be in liquid form.

[0252] The composition can be fabricated in bioreactors such as continuous stirred tank reactors, batch reactors, and on the farm. In some examples, compositions can be stored in a container, such as a jug or in mini bulk. In some examples, compositions may be stored within an object selected from the group consisting of a bottle, jar, ampule, package, vessel, bag, box, bin, envelope, carton, container, silo, shipping container, truck bed, and / or case.

[0253] In some examples, one or more compositions may be coated onto a seed. In some examples, one or more compositions may be coated onto a seedling. In some examples, one or more compositions may be coated onto a surface of a seed. In some examples, one or more compositions may be coated as a layer above a surface of a seed. In some examples, a composition that is coated onto a seed may be in liquid form, in dry product form, in foam form, in a form of a slurry of powder and water, or in a flowable seed treatment. In some examples, one or more compositions may be applied to a seed and / or seedling by spraying, immersing, coating, encapsulating, and / or dusting the seed and / or seedling with the one ormore compositions. In some examples, multiple bacteria or bacterial populations can be coated onto a seed and / or a seedling of the plant. In some examples, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, or more than ten bacteria of a bacterial combination can be selected from any one of the microbes disclosed herein.

[0254] In some embodiments, one or more compositions may be applied. In some embodiments, a composition is applied by way of spraying onto a plant, dripping onto a plant, applying as a seed coat, applying to a field that will then be planted with seed, applying to a field already planted with seed, and / or applying to a field with adult plants. In some embodiments, a composition is applied to seed, plant, soil, or combinations thereof. In some embodiments, a composition is applied to plant parts (e.g., shoot, root, leaves, flower, fruit, and seed). In some embodiments, a composition is applied to above-ground plant parts. In some embodiments, a composition is applied to below-ground plant parts. In some embodiments, a composition of the disclosure is capable of enhancing nematicide function of a target microbe.

[0255] Examples of compositions may include seed coatings for commercially important agricultural crops, for example, sorghum, canola, tomato, strawberry, barley, rice, maize, and wheat. Examples of compositions can also include seed coatings for corn, soybean, canola, sorghum, potato, rice, vegetables, cereals, and oilseeds. Seeds as provided herein can be genetically modified organisms (GMO), non-GMO, organic, or conventional. In some examples, compositions may be sprayed on the plant aerial parts, or applied to the roots by inserting into furrows in which the plant seeds are planted, watering to the soil, or dipping the roots in a suspension of the composition. In some examples, compositions may be dehydrated in a suitable manner that maintains cell viability and the ability to artificially inoculate and colonize host plants. The bacterial species may be present in compositions at a concentration of between lOs to lOio CFU / ml. In some examples, compositions may be supplemented with trace metal ions, such as molybdenum ions, iron ions, manganese ions, or combinations of these ions. The concentration of ions in examples of compositions as described herein may between about 0.1 Mm and about 50 Mm. Some examples of compositions may also be formulated with a carrier, such as beta-glucan, carboxylmethyl cellulose (CMC), bacterial extracellular polymeric substance (EPS), sugar, animal milk, or other suitable carriers. In some examples, peat or planting materials can be used as a carrier, or biopolymers in which a composition is entrapped in the biopolymer can be used as a carrier. The compositions comprising the bacterial populations described herein can improve plant traits, such aspromoting plant growth, maintaining high chlorophyll content in leaves, increasing fruit or seed numbers, and increasing fruit or seed unit weight.

[0256] The compositions comprising the bacterial populations described herein may be coated on to the surface of a seed. As such, compositions comprising a seed coated with one or more bacteria described herein are also contemplated. The seed coating can be formed by mixing the bacterial population with a porous, chemically inert granular carrier. Alternatively, the compositions may be inserted directly into the furrows into which the seed is planted or sprayed onto the plant leaves or applied by dipping the roots into a suspension of the composition. An effective amount of the composition can be used to populate the sub-soil region adjacent to the roots of the plant with viable bacterial growth, or populate the leaves of the plant with viable bacterial growth. In general, an effective amount is an amount sufficient to result in plants with improved traits (e.g. a desired level of nitrogen fixation).

[0257] In some embodiments, the microbes, or microbial compositions of the present disclosure may be formulated using an agriculturally acceptable carrier. The formulation useful for these embodiments may include at least one member selected from the group consisting of a tackifier, a microbial stabilizer, a fungicide, a biopesticide, an antibacterial agent, a preservative, a stabilizer, a surfactant, an anti-complex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a fertilizer, a rodenticide, a dessicant, a bactericide, a nutrient, a hormone, or any combination thereof. In some examples, compositions may be shelf-stable. For example, any of the compositions described herein can include an agriculturally acceptable carrier (e.g., one or more of a fertilizer such as a nonnaturally occurring fertilizer, an adhesion agent such as a non- naturally occurring adhesion agent, and a pesticide such as a non-naturally occurring pesticide). A non-naturally occurring adhesion agent can be, for example, a polymer, copolymer, or synthetic wax. For example, any of the coated seeds, seedlings, or plants described herein can contain such an agriculturally acceptable carrier in the seed coating. In any of the compositions or methods described herein, an agriculturally acceptable carrier can be or can include a non-naturally occurring compound (e.g., a non-naturally occurring fertilizer, a non-naturally occurring adhesion agent such as a polymer, copolymer, or synthetic wax, or a non-naturally occurring pesticide). Non- limiting examples of agriculturally acceptable carriers are described below. Additional examples of agriculturally acceptable carriers are known in the art.

[0258] In some cases, the microbes, or microbial compositions of the present disclosure may be mixed with an agriculturally acceptable carrier. The carrier can be a solid carrier orliquid carrier, and in various forms including microspheres, powders, emulsions and the like. The carrier may be any one or more of a number of carriers that confer a variety of properties, such as increased stability, wettability, or dispersibility. Wetting agents such as natural or synthetic surfactants, which can be nonionic or ionic surfactants, or a combination thereof can be included in the composition. Water-in-oil emulsions can also be used to formulate a composition that includes the isolated bacteria (see, for example, U.S. Patent No. 7,485,451). Suitable formulations that may be prepared include wettable powders, granules, gels, agar strips or pellets, thickeners, and the like, microencapsulated particles, and the like, liquids such as aqueous flowables, aqueous suspensions, water-in-oil emulsions, etc. The formulation may include grain or legume products, for example, ground grain or beans, broth or flour derived from grain or beans, starch, sugar, or oil.

[0259] In some embodiments, the agricultural carrier may be soil or a plant growth medium. Other agricultural carriers that may be used include water, fertilizers, plant-based oils, humectants, or combinations thereof. Alternatively, the agricultural carrier may be a solid, such as diatomaceous earth, loam, silica, alginate, clay, bentonite, vermiculite, seed cases, other plant and animal products, or combinations, including granules, pellets, or suspensions. Mixtures of any of the aforementioned ingredients are also contemplated as carriers, such as but not limited to, pesta (flour and kaolin clay), agar or flour-based pellets in loam, sand, or clay, etc. Formulations may include food sources for the bacteria, such as barley, rice, or other biological materials such as seed, plant parts, sugar cane bagasse, hulls or stalks from grain processing, ground plant material or wood from building site refuse, sawdust or small fibers from recycling of paper, fabric, or wood.

[0260] For example, a fertilizer can be used to help promote the growth or provide nutrients to a seed, seedling, or plant. Non-limiting examples of fertilizers include nitrogen, phosphorous, potassium, calcium, sulfur, magnesium, boron, chloride, manganese, iron, zinc, copper, molybdenum, and selenium (or a salt thereof). Additional examples of fertilizers include one or more amino acids, salts, carbohydrates, vitamins, glucose, NaCl, yeast extract, NH4H2PO4, (NFUkSCri, glycerol, valine, L-leucine, lactic acid, propionic acid, succinic acid, malic acid, citric acid, KH tartrate, xylose, lyxose, and lecithin. In one embodiment, the formulation can include a tackifier or adherent (referred to as an adhesive agent) to help bind other active agents to a substance (e.g., a surface of a seed). Such agents are useful for combining bacteria with carriers that can contain other compounds (e.g., control agents that are not biologic), to yield a coating composition. Such compositions help create coatings aroundthe plant or seed to maintain contact between the microbe and other agents with the plant or plant part. In one embodiment, adhesives are selected from the group consisting of: alginate, gums, starches, lecithins, formononetin, polyvinyl alcohol, alkali formononetinate, hesperetin, polyvinyl acetate, cephalins, Gum Arabic, Xanthan Gum, Mineral Oil, Polyethylene Glycol (PEG), Polyvinyl pyrrolidone (PVP), Arabino-galactan, Methyl Cellulose, PEG 400, Chitosan, Polyacrylamide, Polyacrylate, Polyacrylonitrile, Glycerol, Triethylene glycol, Vinyl Acetate, Gellan Gum, Polystyrene, Polyvinyl, Carboxymethyl cellulose, Gum Ghatti, and polyoxyethylene-polyoxybutylene block copolymers.

[0261] In some embodiments, the adhesives can be, e.g. a wax such as carnauba wax, beeswax, Chinese wax, shellac wax, spermaceti wax, candelilla wax, castor wax, ouricury wax, and rice bran wax, a polysaccharide (e.g., starch, dextrins, maltodextrins, alginate, and chitosans), a fat, oil, a protein (e.g., gelatin and zeins), gum arables, and shellacs. Adhesive agents can be nonnaturally occurring compounds, e.g., polymers, copolymers, and waxes. For example, nonlimiting examples of polymers that can be used as an adhesive agent include: polyvinyl acetates, polyvinyl acetate copolymers, ethylene vinyl acetate (EVA) copolymers, polyvinyl alcohols, polyvinyl alcohol copolymers, celluloses (e.g., ethylcelluloses, methylcelluloses, hydroxymethylcelluloses, hydroxypropylcelluloses, and carboxymethylcelluloses), polyvinylpyrolidones, vinyl chloride, vinylidene chloride copolymers, calcium lignosulfonates, acrylic copolymers, polyvinylacrylates, polyethylene oxide, acylamide polymers and copolymers, polyhydroxyethyl acrylate, methylacrylamide monomers, and polychloroprene.

[0262] In some examples, one or more of the adhesion agents, anti-fungal agents, growth regulation agents, and pesticides (e.g., insecticide) are non-naturally occurring compounds (e.g., in any combination). Additional examples of agriculturally acceptable carriers include dispersants (e.g., polyvinylpyrrolidone / vinyl acetate PVPIVA S-630), surfactants, binders, and filler agents. The formulation can also contain a surfactant. Nonlimiting examples of surfactants include nitrogen-surfactant blends such as Prefer 28 (Cenex), Surf-N(US), Inhance (Brandt), P-28 (Wilfarm) and Patrol (Helena); esterified seed oils include Sun-It II (AmCy), MSO (UAP), Scoil (Agsco), Hasten (Wilfarm) and Mes-100 (Drexel); and organo-silicone surfactants include Silwet L77 (UAP), Silikin (Terra), Dyne-Amic (Helena), Kinetic (Helena), Sylgard 309 (Wilbur-Ellis) and Century (Precision). In one embodiment, the surfactant is present at a concentration of between 0.01% v / v to 10% v / v. In another embodiment, the surfactant is present at a concentration of between 0.1% v / v to 1% v / v.

[0263] In certain cases, the formulation includes a microbial stabilizer. Such an agent can include a desiccant, which can include any compound or mixture of compounds that can be classified as a desiccant regardless of whether the compound or compounds are used in such concentrations that they in fact have a desiccating effect on a liquid inoculant. Such desiccants are ideally compatible with the bacterial population used, and should promote the ability of the microbial population to survive application on the seeds and to survive desiccation. Examples of suitable desiccants include one or more of trehalose, sucrose, glycerol, and Methylene glycol. Other suitable desiccants include, but are not limited to, non-reducing sugars and sugar alcohols (e.g., mannitol or sorbitol). The amount of desiccant introduced into the formulation can range from about 5% to about 50% by weight / volume, for example, between about 10% to about 40%, between about 15% to about 35%, or between about 20% to about 30%. In some cases, it is advantageous for the formulation to contain agents such as a fungicide, a biopesticide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, bactericide, or a nutrient. In some examples, agents may include protectants that provide protection against seed surface-borne pathogens. In some examples, protectants may provide some level of control of soil-borne pathogens. In some examples, protectants may be effective predominantly on a seed surface.Methods of Improving Soil and Promoting Plant Growth

[0264] The disclosure provides methods of producing an improved soil for plant growth, comprising: applying any one of the compositions disclosed herein to soil, thereby producing the improved soil for plant growth. In some embodiments, the method comprises allowing a plant to grow in the improved soil.

[0265] In some embodiments, the growth of the plant is more enhanced in the improved soil, as compared to the growth of the plant in a negative control soil, wherein the composition is not applied to the negative control soil. In some embodiments, the method inhibits a plant pathogen in the improved soil.

[0266] In some embodiments, a method comprises application of a composition (i.e., a composition comprising a microbial signaler and / or a target microbe) of the disclosure. In some embodiments, a method comprises application of a composition to a seed, a plant, a plant part, soil, and / or the soil surrounding the seed and / or the plant. In some embodiments, a method comprises application of a composition to soil, thereby producing improved soil. In some embodiments, a method comprises enhancing nematicide function of a target microbe. In someembodiments, a method comprises increasing plant root biomass and / or shoot biomass. In some embodiments, a method comprises decreasing SCN female counts, SCN egg counts, RLN female counts, RLN egg counts, RKN female counts, RKN egg counts, root lesion counts and / or root galling counts. In some embodiments, a method comprises increasing plant root biomass and / or shoot biomass and decreasing SCN female counts, SCN egg counts, RLN female counts, RLN egg counts, RKN female counts, RKN egg counts, root lesion counts and / or root galling counts. In some embodiments, a method comprises application of a composition by way of spraying onto a plant, dripping onto a plant, applying as a seed coat, applying to a field that will then be planted with seed, applying to a field already planted with seed, and / or applying to a field with adult plants. In some embodiments, a method comprises application of a composition to seed, plant, soil, or combinations thereof. In some embodiments, a method comprises application of a composition to plant parts (e.g., shoot, root, leaves, flower, fruit, and seed). In some embodiments, a method comprises application of a composition to above-ground plant parts. In some embodiments, a method comprises application of a composition to below-ground plant parts. In some embodiments, a method comprises contacting a target microbe with a microbial signaler of a composition disclosed herein. In some embodiments, contacting a target microbe with a microbial signaler of a composition occurs prior to application. In some embodiments, contacting a target microbe with a microbial signaler of a composition occurs concurrently with application. In some embodiments, contacting a target microbe with a microbial signaler of a composition occurs after application.

[0267] In some embodiments, the growth of the plant is more enhanced in the improved soil, as compared to the growth of the plant in a negative control soil, wherein the composition is not applied to the negative control soil. In some embodiments, the method inhibits a plant pathogen in the improved soil. In some embodiments, the inhibition of a plant pathogen in the improved soil is higher than in a negative control soil, wherein the composition is not applied to the negative control soil. In some embodiments, the inhibition of a plant pathogen in the improved soil is at least about 1% (for example, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, or about 100%, including all values and subranges that lie therebetween) higher than in a negativecontrol soil, wherein the composition is not applied to the negative control soil. In some embodiments, the inhibition of a plant pathogen in the improved soil is about 1-5%, about 1- 10%, about 2-10%, about 5-15%, about 10-20%, about 15-30%, about 25-50%, about 40-60%, about 50-70%, about 50-80%, about 60-80%, about 80-100%, about 1-100%, abo...

Claims

CLAIMSWhat is claimed is:

1. A composition, comprising: at least one microbial signaler, wherein the at least one microbial signaler is capable of enhancing a plant growth-promoting function of at least one target microbe.

2. The composition of claim 1, wherein the at least one microbial signaler is a species of any one of: Streptomyces angustmyceticus, Streptomyces atrolaccus, Streptomyces aurcitus, Streptomyces echinatus, Streptomyces catenulae, Streptomyces cine reus. Streptomyces cinnamonensis. Streptomyces flaveus, Streptomyces ginsengisoli, Streptomyces graminisoli. Streptomyces griseocarneus. Streptomyces gulbargensis. Streptomyces hygroscopicns . Streptomyces lavendulae, Streptomyces libani, Streptomyces longwoodensis, Streptomyces lucensis. Streptomyces lydicus, Streptomyces nigrescens. Streptomyces nojiriensis. Streptomyces platensis, Streptomyces rimosus, Streptomyces sioyaensis, Streptomyces spororaveus, Streptomyces tanashiensis, Streptomyces tubercidicus, Streptomyces venezuelae, Streptomyces virginiae. Streptomyces xanthophaeus, Streptomyces yaanensis. or any combination thereof.

3. The composition of claim 1, comprising: at least one target microbe.

4. The composition of claim 1, comprising: at least two target microbes.

5. The composition of claim 1, comprising: at least two microbial signalers.

6. The composition of claim 1, wherein the at least one target microbe is a genus ofBacillus, Bradyrhizobium, Delftia, Rhizophagus, or a combination thereof, optionally wherein the at least one target microbe is a species selected from any one of Bacillus amyloliquefaciens, Bacillus subtilis, Bradyrhizobium elkanii, Bradyrhizobium japonicum, Delftia acidovorans, Rhizophagus irregularis, or a combination thereof7. The composition of claim 1, wherein the plant growth-promoting function of a target microbe is selected from any one of: (a) plant pathogen-inhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, (e)nitrogen fixation, (f) nutrient acquisition, (g) production of plant growth hormones, (h) a nematicide function, or (i) any combination thereof.

8. The composition of claim 1, wherein the plant growth-promoting function is a nematicide function.

9. The composition of claim 1, wherein the plant growth-promoting function is a nematicide function and wherein said function comprises one or more of increasing plant root biomass, increasing shoot biomass, decreasing SCN female counts, decreasing SCN egg counts, decreasing RLN female counts, decreasing RLN egg counts, decreasing RKN female counts, decreasing RKN egg counts, decreasing root lesion counts, and decreasing root galling counts.

10. The composition of claim 1, wherein the at least one target microbe is from a genus of any one of Bacillus, Burkholderia, Streptomyces, and Paecilomyces.

11. The composition of claim 1, wherein the at least one target microbe is a species of any one of Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus subtilis, Bacillus thuringiensis, Bacillus velenzensis, Burkholderia amyloliquefaciens, Burkholderia rinojensis, and Paecilomyces lilacinum.

12. The composition of claim 1, wherein the at least one microbial signaler is capable of enhancing a nematicide function of at least one target microbe against a nematode having a genus of any one of Belonolaimus, Heterodera, Hoplolaimus, Meloidogyne, Pratylenchus, and Rotylenchulus .

13. The composition of any one of claims 1-12, comprising: at least two microbial signalers and / or at least two target microbes.

14. A composition, comprising: at least one microbial signaler, wherein the at least one microbial signaler is capable of enhancing a nematicide function of at least one target microbe.

15. The composition of claim 14, comprising: at least one target microbe.

16. The composition of claim 14, comprising: at least two target microbes.

17. The composition of claim 14, comprising: at least two microbial signalers.

18. The composition of claim 14, wherein enhancing nematicide function comprises one or more of increasing plant root biomass, increasing shoot biomass, decreasing SCN female counts, decreasing SCN egg counts, decreasing RLN female counts, decreasing RLN egg counts, decreasing RKN female counts, decreasing RKN egg counts, decreasing root lesion counts, and decreasing root galling counts.

19. The composition of claim 14, wherein the at least one target microbe is from a genus of any one of Bacillus, Burkholderia, Streptomyces, and Paecilomyces.

20. The composition of claim 14, wherein the at least one target microbe is a species of any one of Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus subtilis, Bacillus thuringiensis, Bacillus velenzensis, Burkholderia amyloliquefaciens, Burkholderia rinojensis, and Paecilomyces lilacinum.

21. The composition of claim 14, wherein the at least one microbial signaler is capable of enhancing a nematicide function of at least one target microbe against a nematode having a genus of any one of Belonolaimus, Heterodera, Hoplolaimus, Meloidogyne, Pratylenchus, and Rotylenchulus .

22. The composition of any one of claims 14-21, comprising: at least two microbial signalers and / or at least two target microbes.

23. A method of enhancing nematicide function of at least one target microbe, the method comprising: bringing the at least one target microbe in the physical proximity of the composition of claim 14.

24. The method of claim 23, wherein enhancing nematicide function comprises one or more of increasing plant root biomass, increasing shoot biomass, decreasing SCN female counts, decreasing SCN egg counts, decreasing RLN female counts, decreasing RLN egg counts,decreasing RKN female counts, decreasing RKN egg counts, decreasing root lesion counts, and decreasing root galling counts.

25. The method of claim 23, wherein the at least one target microbe is from a genus of any one of Bacillus, Burkholderia, Streptomyces, and Paecilomyces.

26. The method of claim 23, wherein the at least one target microbe is a species of any one of Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus subtilis, Bacillus thuringiensis, Bacillus velenzensis, Burkholderia amyloliquefaciens, Burkholderia rinojensis, and Paecilomyces lilacinum.

27. The method of claim 23, wherein the at least one microbial signaler is capable of enhancing a nematicide function of at least one target microbe against a nematode having a genus of any one of Belonolaimus, Heterodera, Hoplolaimus, Meloidogyne, Pratylenchus, and Rotylenchulus .

28. The method of claim 23, wherein the at least one microbial signaler is a species selected from any one of Streptomyces avidinii, Streptomyces colombiensis, Streptomyces lavendulae, Streptomyces roseochromogenus, Streptomyces spororaveus, Streptomyces sporoverrucosus, Streptomyces venezuelae, Streptomyces xanthophaeus, Streptomyces angustmyceticus, Streptomyces hygroscopicus, Streptomyces libani, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces platensis, Streptomyces rimosus, Streptomyces tubercidicus, Streptomyces bungoensis, Streptomyces cyslabdanicus, Streptomyces galbus, Streptomyces kagawaensis, Streptomyces lasaliensis, Streptomyces lasalocidi, Streptomyces longwoodensis, Streptomyces spinichromogenes, Streptomyces cirratus, Streptomyces nojiriensis, Streptomyces verne, Streptomyces vinaceus, Streptomyces virginiae, Streptomyces catenulae, Streptomyces cinereus, Streptomyces griseocarneus, Streptomyces sioyaensis, Streptomyces subrutilus, Streptomyces atrolaccus, Streptomyces auratus, Streptomyces fagopyri, Streptomyces kaempferi, Streptomyces mirabilis, Streptomyces olivochromogenes, Streptomyces chattanoogensis, Streptomyces flaveus, Streptomyces goshikiensis, Streptomyces cinnamonensis, Streptomyces senoensis, Streptomyces echinatus, Streptomyces fdipinensis, Streptomyces gulbargensis, Streptomyces myxogenes, Streptomyces novaecaesareae, Streptomyces spectabilis, Streptomyces tanashiensis, Streptomyces ginsengisoli, Streptomycesgraminisoli, Streptomyces lucensis, Streptomyces yaanensis, Streptomyces caniferus, Streptomyces decoyicus, Streptomyces glebosus, Streptomyces ossamyceticus, Streptomyces badius, Streptomyces cyaneofuscatus, Streptomyces flavogriseus, Streptomyces griseus, Streptomyces mediolani, Streptomyces praecox, Streptomyces pratensis, Streptomyces omiyaensis, Streptomyces aquilus, Streptomyces caeruleatus, Streptomyces griseochromogenes, Streptomyces pseudovenezuelae, Streptomyces viridochromogenes, Streptomyces argenteolus, Streptomyces chrestomyceticus, Streptomyces coelicolor, Streptomyces microsporus, Streptomyces aureus, Streptomyces lutosisoli, Streptomyces minoensis, Streptomyces rhizosphaerihabitans, Streptomyces griseoruber, Streptomyces scabiei, Streptomyces achromogenes, Streptomyces canarius, Streptomyces capoamus, Streptomyces cellostaticus, or Streptomyces katrae.

29. The method of claim 23, wherein the at least one microbial signaler is a species selected from any one of: Streptomyces angustmyceticus , Streptomyces atrolaccus, Streptomyces auratus, Streptomyces echinatus, Streptomyces catenulae, Streptomyces cinereus, Streptomyces cinnamonensis, Streptomyces flaveus, Streptomyces ginsengisoli, Streptomyces graminisoli, Streptomyces griseocarneus, Streptomyces gulbargensis, Streptomyces hygroscopicus , Streptomyces lavendulae, Streptomyces libani, Streptomyces longwoodensis, Streptomyces lucensis, Streptomyces lydicus, Streptomyces nigrescens, Streptomyces nojiriensis, Streptomyces platensis, Streptomyces rimosus, Streptomyces sioyaensis, Streptomyces spororaveus, Streptomyces tanashiensis, Streptomyces tubercidicus, Streptomyces venezuelae, Streptomyces virginiae, Streptomyces xanthophaeus , and Streptomyces yaanensis30. A method of enhancing a plant growth-promoting function of at least one target microbe, comprising: administering a composition of claim 1.

31. The method of claim 30, wherein the composition comprises: the at least one target microbe.

32. The method of claim 30, wherein the composition comprises: at least two microbial signalers and / or at least two target microbes.

33. The method of claim 30, wherein the at least one target microbe has a genus of Bacillus, Bradyrhizobium, Delftia, Rhizophagus, or a combination thereof, optionally wherein the atleast one target microbe is a species selected from any one of Bacillus amyloliquefaciens, Bacillus sublilis. Bradyrhizobium elkanii, Bradyrhizobium japonicum, Delftia acidovorans, Rhizophagus irregularis, or any combination thereof34. The method of claim 30, wherein the plant growth-promoting function of a target microbe is selected from any one of: (a) plant pathogen-inhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, (e) nitrogen fixation, (f) nutrient acquisition, (g) production of plant growth hormones, (h) a nematicide function, or (i) any combination thereof.

35. A method of improving plant root biomass, shoot biomass, plant growth, plant vigor, stand count, or nodulization, comprising: applying a composition of any one of claim 1-22 to a seed, or soil around the seed, or to a plant.

36. The method of claim 35, wherein the composition, comprises: the at least one target microbe.

37. The method of claim 35, wherein the composition comprises: at least two microbial signalers and / or at least two target microbes.

38. The method of claim 35, wherein the at least one target microbe has a genus of Bacillus, Bradyrhizobium, Delftia, Rhizophagus, or a combination thereof, optionally wherein the at least one target microbe is a species selected from any one of Bacillus amyloliquefaciens, Bacillus subtilis, Bradyrhizobium elkanii, Bradyrhizobium japonicum, Delftia acidovorans, Rhizophagus irregularis, or any combination thereof39. The method of claim 35, wherein the composition improves a plant growth-promoting function of a target microbe, and wherein the plant-growth promoting function is selected from any one of: (a) plant pathogen-inhibiting function, (b) zinc solubilizing function, (c) phosphate solubilizing function, (d) production of an antibiotic, (e) nitrogen fixation, (f) nutrient acquisition, (g) production of plant growth hormones, (h) a nematicide function, or (i) any combination thereof.