Bacteria as a carrier to deliver nucleic acid vectors

Abstract

The present invention relates to the use of bacteria, such as Salmonella, as a carrier for an expression vector that encodes all or a portion of replicon proteins from a positive stranded virus, wherein expression of the replicon proteins is under the control of eukaryotic and / or prokaryotic promoters, and wherein expression of a payload is under the control of a sub-genomic promoter. Also provided are methods of using the bacteria comprising the vector in therapeutics and vaccines.

Description

[0001] BACTERIA AS A CARRIER TO DELIVER NUCLEIC ACID VECTORS

[0002] FIELD OF THE INVENTION

[0003] This invention generally pertains to bacteria as a carrier to deliver nucleic acid vectors. In particular, the invention pertains to attenuated Salmonella to deliver self amplifying nucleic acid vectors and their uses as a therapeutic or vaccine.

[0004] BACKGROUND OF THE INVENTION

[0005] WO2022 / 246559 and PCT / CA2023 / 051444 (herein incorporated by reference) teach self-amplifying expression vectors that encode all or a portion of replicon proteins from a positive stranded virus. Also taught are methods of using the vector in therapeutics and vaccines. Such nucleic acid expression vectors may be delivered unencapsulated (i.e. as a naked vector) or may be incorporated into liposomes, microspheres or other polymer matrices.

[0006] Live bacteria are being explored as carriers for orally administered vaccines. The mucosal surface of the gastrointestinal (Gl) tract is the first line of defense against foreign pathogens and toxins ingested orally. The content of the Gl tract is constantly being sampled by the immune system through specialized epithelial cells known as M-cells, which are present in the Peyer's patches of the gut, providing a thin covering over lymphoid tissue. In this way, once a harmful entity is found the immune system can be immediately activated to kick in the immune response and eliminate the threat.

[0007] Salmonella has been used as a carrier for nucleic acid vectors for use in vaccines and cancer therapeutics. See for example US8,703,153 which teaches Salmonella vectored vaccines against Chlamydia and methods of use. WO2023 / 235633 teaches Salmonella vectored therapies for treatment of cancer. Chabloz et al (Commun Biol 3, 342 (2020). https: / / doi.orq / 10.1038 / s42003-020-1072-4) teaches a Salmonellabased platform for efficient delivery of functional binding proteins to the cytosol.

[0008] Salmonella infect the immune cells in the gut and is immunogenic since they possess Lipopolysaccharide (LPS) and flagellins as biological adjuvants which are agonists for TLR4 and TLR5 / TRL11 respectively. This background information is provided for the purpose of making known information believed by the applicant to be of possible relevance to the present invention. No admission is necessarily intended, nor should be construed, that any of the preceding information constitutes prior art against the present invention.

[0009] SUMMARY OF THE INVENTION

[0010] An object of the present invention is to provide bacteria as a carrier to deliver nucleic acid vectors. In accordance with an aspect of the present invention, there is provided an attenuated Salmonella bacteria comprising a self-amplifying DNA vector encoding one or more payloads, optionally wherein said self-amplifying DNA vector is capable of expressing said one or more payloads (a) only when delivered to a eukaryotic cell; (b) only in said bacteria; or (c) in an eukaryotic cell and said bacteria. In certain embodiments, the self-amplifying DNA vector comprises: one or more promoters; replicon protein genes from Venezuelan equine encephalitis (VEE) virus under the control of the promoter; a sub-genomic promoter from the VEE virus, a nucleic acid encoding a payload under the control of the sub-genomic promoter.

[0011] In accordance with an aspect of the present invention, there is provided an attenuated Gram-negative bacteria comprising a self-amplifying nucleic acid vector encoding one or more payloads, optionally wherein said self-amplifying nucleic acid vector is capable of expressing said one or more payloads: a) only when delivered to a eukaryotic cell; b) only in said bacteria; or c) in a eukaryotic cell and said bacteria. In certain embodiments, the gram-negative bacteria is selected from Salmonella, Escherichia coli, Shigella, Yersinia, or Pseudomonas. In certain embodiments, the self-amplifying nucleic acid vector comprises: a) one or more promoters; b) a replicon system enabling autonomous amplification of said nucleic acid within a eukaryotic cell and / or bacteria; c) a sub-genomic promoter for controlled expression; d) a nucleic acid payload of up to 20kb under the control of said sub-genomic promoter. In certain embodiments, the self-amplifying nucleic acid vector is a) a self-amplifying RNA vector system; or b) a self-amplifying DNA vector system. In certain embodiments, replicon system is derived from a virus selected from a) Alphaviruses (Venezuelan equine encephalitis virus (VEEV), Sindbis virus, Semliki Forest virus);b) Flaviviruses (Dengue virus, Zika virus, Yellow Fever virus);c) Paramyxoviruses (Sendai virus, Measles virus); and d) Rhabdoviruses (Vesicular stomatitis virus, Rabies virus). In accordance with an aspect of the present invention, there is provided a pharmaceutical composition comprising the Salmonella bacteria of the present invention and a pharmaceutically acceptable carrier. In certain embodiments, the pharmaceutical composition is formulated for oral administration.

[0012] In accordance with an aspect of the present invention, there is provided a method of treating, and / or preventing disease in a subject, said method comprising orally administering one or more of the bacteria of the present invention, wherein said vector expresses a therapeutic polypeptide effective against said disease, optionally wherein each of said one or more of the bacteria express one or more different payloads; and optionally in combination with a further therapeutic.

[0013] In accordance with an aspect of the present invention, there is provided a method of stimulating an antigen-specific immune response, said method comprising administering said method comprising orally administering the bacteria of the present invention, wherein said vector expresses one or more immunogens or epitopes from an infectious agent or cancer cell expressing the antigen.

[0014] In certain embodiments of the methods, administering comprises oral delivery; intravenous injection; intranasal administration; intramuscular injection; and / or sublingual administration.

[0015] In certain embodiments of the methods, the bacteria are administered in a single dose, multiple doses, or a time-course regimen. In certain embodiments of the methods, the dose is administered at regular intervals, optionally wherein the dosing schedule is selected based on the therapeutic need and the pharmacokinetics of the bacteria. In certain embodiments of the methods, the time-course of administration involves a series of escalating doses, optionally with increased frequency or dosage over time to achieve optimal therapeutic effects.

[0016] In certain embodiments of the methods, the subject is a mammal, optionally a human, a reptile, a bird, an amphibian, or a fish.

[0017] BRIEF DESCRIPTION OF THE FIGURES These and other features of the invention will become more apparent in the following detailed description in which reference is made to the appended drawings.

[0018] Figure 1 illustrates antibody response to target antigen in mouse plasma 28 days post-administration: IgG response. The antibody responses were also compared to the normal saline injected control (data not shown). Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). One way Analysis of Variance (ANOVA) statistical test was conducted between the groups. Naked Self-amplifying DNA vaccine shows robust immune response when administered using various routes of inoculation. Salmonella vaccine is Self-amplifying (sa)DNA packaged in Salmonella bacteria.

[0019] Figure 2 provides a comparison between antibody response to target antigen to low and high concentration of target antigen coated on the ELISA plate. Salmonella alone control group was compared with Naked Self-amplifying DNA Salmonella vaccine with serum samples collected 42 days post-administration: IgG response. The antibody responses were also compared to the normal saline injected control (data not shown). Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). Paired t test was conducted between the control and the Salmonella vaccine groups. This data demonstrates that high antigen coating reduces non-specific background levels and increases antigen-specific antibody responses in ELISA

[0020] Figure 3 illustrates antibody response to target antigen for different routes of inoculation of naked self-amplifying DNA (100 pg) vaccine in mouse feces 28 days post-administration: IgG response. The antibody responses were also compared to the normal saline injected control (data not shown). Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). One way Analysis of Variance (ANOVA) statistical test was conducted between the groups. The data demonstrate saDNA (self amplifying) Salmonella Vaccine induces high titer of mucosal antibodies.

[0021] Figure 4 illustrates neutralizing antibody response to SARS-Cov-2 was compared between mRNA vaccine and the naked self-amplifying DNA Salmonella vaccine to SARS-CoV-2 using serum samples 42 days post-administration. The antibody responses were also compared to the normal saline injected control (data not shown). The data was plotted on a log scale and paired t test was conducted between the groups. The data demonstrate saDNA Salmonella vaccine to SARS- CoV-2 is superior to mRNA vaccine in inducing high titers of neutralizing antibodies.

[0022] Figure 5a-c illustrates antibody response to target antigen for different routes of inoculation of naked self-amplifying DNA (100 pg) vaccine in mouse plasma 28 (fig 5a), 42 (fig 5b) and 156 (fig 5c) days post-administration: IgG response. The antibody responses were also compared to the normal saline injected control (data not shown). Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). One way Analysis of Variance (ANOVA) statistical test was conducted between the groups. The data demonstrate that saDNA Salmonella vaccine and the saDNA Naked vaccine (intramuscular route) induces strong and long-lasting antibody responses compared to the other routes of inoculation.

[0023] Figure 6 illustrates Mini-Prep of Gemini vector DNA and subsequently RT-PCR on mRNA from the attenuated Salmonella strain, SL1344 transformed with selfamplifying vectors with Cov-2 omicron spike showed they are replicated and transcribed in the Salmonella.

[0024] Figure 7 illustrates Salmonella can infect cells, deliver self-amplifying vector DNA, release it in the cytoplasm and express protein in macrophage cell line (RAW264.7) 48 to 72 hr post infection.

[0025] Figure 8 illustrates ELISA Data to show the anti-Cov-S antibody production from Salmonella carrying the self-amplifying vector pDNA with Cov-2 Spike S after administration through oral gavage. Infecting mice by gavaging the attenuated Salmonella with self-amplifying vectorDNA expressing Cov-2 Spike S in vivo study). 5X106 cells used (equivalent to about 3.1 ng to 6.3ng) GD600 of 1.0 = 8 x 108cells / ml. Typical DNA yield of the construct from Mini-prep of the O / N culture (OD=1): 5 to 10ug 5X106 Salmonella have approximately 3.1 ng to 6.3ng (less than 0.01 ug; lower than other groups used for the LNP-mRNA-Cov-S vaccines. The data demonstrate that the oral route of inoculation induced antigen-specific antibody responses. Salmonella vaccine induced higher antibody responses compared to the naked saDNA oral vaccine

[0026] Figure 9 illustrates the vector map of self-amplifying vector CMV-T7-Vee- Opti_OmicronS. Figure 10 illustrates process with a vector which expresses payload in eukaryotic cells.

[0027] Figure 11 illustrates process with a vector which expresses payload in prokaryotic cells.

[0028] Figure 12 illustrates process with a vector which expresses payload in prokaryotic and eukaryotic cells.

[0029] Figure 13 illustrates the vector configuration allowing for prokaryotic expression (top), eukaryotic expression (middle), and both prokaryotic and eukaryotic expression (bottom of the payload).

[0030] Figure 14 illustrates the vector map of an exemplary vector capable of expressing only in prokaryotic cells.

[0031] Figure 15 illustrates the vector map of an exemplary vector capable of expressing only in eukaryotic cells.

[0032] Figure 16 illustrates the vector map of an exemplary vector capable of expressing in both prokaryotic and eukaryotic cells.

[0033] Figure 17 illustrates the vector platform design: Nucleic acid-based vaccines offer rapid development and manufacturing. The self-amplifying vector set forth in Figure 2), resembles the alphavirus backbone from the Venezuelan Equine Encephalitis (VEE) virus 5-7, which allow amplification of the vector but no viral particle formation. The vector platform boasts an engineered alphavirus replicon, ensuring robust antigen expression. Its construct includes a dual promoter (CMV and T7), replicon protein genes (NSP1-4), a 26S sub-genomic promoter from VEE, and resistance genes for puromycin (PuroR) and ampicillin (AmpR) for various cell cultures. Gemini can manifest as saRNA or saDNA.

[0034] Figure 18 provides the construct map of CAG+T7_VEE_G3_ Eukaryotes + Prokaryotes (Sequence set forth in SEQ ID NO: 7). A new dual expression Self amplifying (SA) vector in Eukaryotes and in Gram negative bacteria like E. coli or Salmonella with Chicken Beta Actin promoter, Bacterial promoter (TRC) I terminator(T7) / Shine Dalgano ribosomal binding site (SD_RBS), VEE conserved regulatory sequences, and Ploy(A) signal for bacterial and eukaryotic transcriptional termination. This vector has Renilla luciferase as a model gene but it can be replaced by a gene for therapeutic drug genes or antigen genes.

[0035] Figure 19 provides the construct map of: CAG+T7_VEE_G3_Eukaryotes (Sequence set forth in SEQ ID NO: 8): Expression vector only in eukaryotic cells. Corresponds to the construct of figure 18 with all the bacterial components are removed. This vector has Renilla luciferase as a model gene but it can be replaced by a therapeutic gene or antigen gene.

[0036] Figure 20 provides the construct map of TRC_VEE_G3_Prokaryotes (SEQ ID NO: 9): only expressed in gram negative bacteria. This vector has Renilla luciferase as a model gene but it can be replaced by a therapeutic gene or antigen gene.

[0037] Figure 21 provides the construct map of CMV+T7_VEE_OmicronS_C2 (SEQ ID NQ:10).

[0038] Figure 22 illustrates that saDNA Salmonella vaccine (map of construct used is in Figure 21) (Oral route) induces strong and Iong4asting antibody responses compared to the Naked DNA group and the control group. Antibody response to different routes of inoculation of Naked Self-amplifying DNA (100 pg) vaccine in mouse plasma up to 112 days post-injection: IgG response. The antibody responses were also compared to the normal saline injected control. Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). 5x105cfu and 5x106cfu doses induced robust antibody response with titers reaching up to 40 micrograms / ml. 5x107cfu and 5x108cfu doses induced very strong antibody response with titers reaching up to 60-80 micrograms / ml. One way Analysis of Variance (ANOVA) statistical test was conducted between the groups.

[0039] Figure 23 illustrates ELISPOT data- Salmonella dose titration experiment. The Y-axis shows the numbers of interferon-gamma spots produced by T cells in response to the peptide stimulation. The data shows a clear dose titration with naked DNA and 5x105Salmonella vaccine group shows least amount of proliferation. 5x106and 5x107Salmonella dose group on the other hand shows the greatest amount of proliferation.

[0040] Figure 24 illustrates comparison between antibody response to Salmonella control and Salmonella-SARS Cov-2 Spike vaccine: IgG response. Salmonella alone control group was compared with Naked Self-amplifying DNA Salmonella vaccine with serum samples collected 42 days post-injection: IgG response. The antibody responses were also compared to the normal saline injected control (data not shown). Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). Paired t test was conducted between the control and the Salmonella vaccine groups.

[0041] Figure 25 illustrates comparison between antibody response to Salmonella control and Salmonella-SARS Cov-2 Spike vaccine: IgG response. Salmonella alone control group was compared with Naked Self-amplifying DNA Salmonella vaccine with serum samples collected 156 days post-injection: IgG response. The antibody responses were also compared to the normal saline injected control (data not shown). Normal serum from age matched unvaccinated K18hAce2 transgenic mice were used as background control in the ELISA (data not shown). Paired t test was conducted between the control and the Salmonella vaccine groups.

[0042] DETAILED DESCRIPTION OF THE INVENTION

[0043] The present inventors, as described in WO2022 / 246559 and PCT / CA2023 / 051444 (incorporated by reference herewith), developed expression vectors that encode all or a portion of replicon proteins from a positive stranded virus, wherein expression of the replicon proteins is under the control of eukaryotic and / or prokaryotic promoters, and the expression of a payload is under the control of a sub-genomic promoter.

[0044] The present invention expands upon the inventor’s previous work. In particular, the present inventors have found that Sa / mone / / a-mediated transfer of the self-amplifying (sa) vectors into mammalian cells (bactofection) resulted in protein expression of the payload in the mammalian cells. In addition, oral inoculation with the Salmonella comprising self-amplifying vectors resulted in enhanced antibody response to target antigen as compared to inoculation of naked vector via various routes. Accordingly, the present invention provides bacteria as a carrier for nucleic acid expression vectors, optionally self-amplifying vectors. The bacteria comprising the nucleic acid expression vector may be used in therapeutics, including but not limited to the use of the vectors in vaccines and immunotherapeutics. In certain embodiments, the bacteria comprising the vectors are for use in mucosal administration, including via oral administration. Bacteria comprising Nucleic Acid Expression Vectors

[0045] The present invention provides bacteria comprising nucleic acid expression vectors capable of delivering the nucleic acid expression vectors into the cytoplasm of eukaryotic cells. In certain embodiments, the bacteria are attenuated. The terms “attenuated” or “attenuation” as used herein refer to the process of rendering certain pathogen virulence attributes needed to cause diseases less able to cause such disease symptoms.

[0046] Appropriate bacteria are known in the art and include but are not limited to Salmonella and E. coll.

[0047] In certain embodiments, the bacteria are Salmonella. Exemplary Salmonella include but are not limited to Salmonella enterica serovar Typhimurium. S. Typhimurium Salmonella enterica serovar Typhi S. Typhi / Salmonella enterica serovar Dublin (S. Dublin), Salmonella enterica serovar Enteritidis (S. Enteritidis). In certain embodiments, the Salmonella is S. Typhimurium. Strains of Salmonella that may be used are known in the art and include SL 1344, x11623, Ty21a-Gad (MD297), AH9, AH12, CWD9, MFA17, x8937(pYA3685),Ty21a-PA-01 , MD149, MD174, MD194, MD196, Ty21a-AR-Ss, LH1160, CKS257, MvP525 (p2810), MvP728 (p3635), SV9699 (plZ2267), CVD 908ssb-TXSVN, CVD 1925 (pSEC10-wzzB), CVD 1943, 618 tolR msbB pagP and 1418 tolR htrB.

[0048] In specific embodiments, the strain of Salmonella is SL1344.

[0049] Methods of introducing nucleic acid expression vectors into the bacteria are known in the art. Such methods include but are not limited to chemical transformation / heat shock and electroporation. In certain embodiments, the nucleic acid expression vectors of the present invention are introduced into attenuated Salmonella via electroporation. In specific embodiments, the Salmonella is strain SL 1344.

[0050] The nucleic acid expression vectors are self-amplifying expression vectors. In some embodiments, the present invention provides expression vectors based on positive stranded viruses belonging to the orders Nidovirales, Martellivirales and Hepelivirales and uses thereof. In particular in certain embodiments, the present invention provides a vector, including but not limited to a self-amplifying plasmid DNA vector, that encodes all or a portion of replicon proteins from a positive virus of interest and includes a multi-cloning site to allow insertion of a sequence of a payload of interest.

[0051] The vectors may be designed to allow for payload expression in prokaryotes, eukaryotes and both prokaryotes and eukaryotes. See figures 10- 13 and 18-21.

[0052] In some embodiments of the invention, the vector is a plasmid DNA vector encoding the replicon from a positive stranded virus where the expression of the replicon proteins is driven by a eukaryotic promoter and / or a prokaryotic promoter or a dual eukaryotic prokaryotic promoter. In some embodiments the promoter is a fused dual eukaryotic prokaryotic promoter. As used herein, the term promoter includes promoters and promoters plus enhancer elements. In specific embodiments, there is provided a vector comprising dual promoter CMV and T7.

[0053] The eukaryotic promoter may be constitutive, inducible or tissue specific. Exemplary eukaryotic promoters include but are not limited to CMV, EF1 a, SV40, PGK1 (human or mouse), Ubc, human beta actin, Chicken Beta Actin promoter, CAG, TRE, UAS, Ac5, Polyhedrin, CaMKIla, GAL1 , 10, TEF1 , GDS, ADH1 , CaMV35S, Ubi, H1 and U6. Exemplary prokaryotic promoters include but are not limited to T7, T7lac, Sp6, TRC, araBAD, trp, lac, Ptac and pL.

[0054] In certain embodiments, the eukaryotic promoter is tissue specific. Exemplary promoters include but are not limited to B29 promoter, CD14 promoter, CD43 promoter, CD45 promoter, CD68 promoter, Desmin promoter, Elastase- 1 promoter, Endoglin promoter, Fibronectin promoter, Flt-1 promoter, GFAP promoter, GPIIb promoter, ICAM-2 promoter, mIFN-p promoter, Mb promoter, Nphsl promoter, OG-2 promoter, SP-B promoter, SYN1 promoter, WASP promoter, SV40 I bAlb promoter, SV40 I hAlb promoter, SV40 I CD43 promoter, SV40 I CD45 promoter and NSE I RU5' promoter.

[0055] In certain embodiments, the tissue specific promoter is selected from the promoters of POMC, B29, CD4, CD14, CD43, CD45, CD68, Desmin, Elastase, Endoglin, Fibronectin, Flt-1 , GFAP, GPIIb, ICAM-2, INF-beta, Mb, Nphsl, OG-2, SP-B, SYN1 , WASP, Alb, RU5', Nos1 , Thy- 1 (Thymocyte differentiation antigen 1 , CD90), Slc6a4PDGF, PDGFRb, CX3CR1 , TRPA1 , Krt5, actb, aMHC, 1a1 kin, Cck, zDC, cFos, Handl , Rosa, Insl5, Cart, Sctr, Ins1 , Nrsnl , Foxp3, Tph, Cnr1 , Pzp, CD23, Cx40, Foxnl , Rspo3, Krt13, Pnoc, ChAT, MMTV, Myh6, Sftpc, Mlc2a, Atf3, Pirt, Dbh, Villin, Vav1 , Sox2, Dat, Pdx1 , Cal, Gfral, Cr, BAF53b, Cntn2, Nav1.8, ObRb, Krt5, Advillin, Mrgprd, PV, Pax7, Calbl , Mx1 , Nmu, Aldhl , CAG, CD19, Krt14, Vil1 , Stra8, E8i, BAF53b, Pf4, UBC, Vip, TCF21 , Cart, Htr3b, Pdx1 , Mgarp, Mx1 , Nmu, GFAP, vGlut2, Tac1.

[0056] In specific embodiments, the vector is a DNA plasmid driven by a CMV promoter with or without a T7 promoter. In such embodiments, once the plasmid enters the cell, the plasmid DNA will drive expression of the positive stranded RNA replicon that will in turn drive replication of the negative strand RNA that will begin the self-amplifying mRNA cycle.

[0057] In more specific embodiments, the vector is a self-amplifying plasmid DNA vector with dual promoter (such as a CMV and T7) which encodes all or a portion of the replicon proteins from the Venezuelan Equine Encephalitis (VEE) virus genome. In this embodiment, the dual promoter will drive transcription in vivo or in vitro of mRNA encoding all the replicon proteins necessary for self-amplification of mRNAs. One or more sub-genomic promoters will drive expression of downstream payloads.

[0058] The complete genome of VEE virus is known in the art and is published under GenBank Accession NC_001449. In certain embodiments of the invention, the vector includes the full viral replicon (i.e. the 5’ leader sequence, followed by the viral replicase gene), followed by the payload, followed by the viral 3’ terminal segment.

[0059] In specific embodiments, the vector comprises a promoter (including but not limited to a binary eukaryotic prokaryotic promoter), replicon protein genes from VEE virus under the control of the binary promoter, a sub-genomic promoter from the VEE virus, multi-cloning site for insertion of a nucleic acid encoding a payload under the control of the sub-genomic promoter and optionally resistance gene(s) for mammalian and bacterial cell culture, respectively. In more specific embodiments, the vector comprises a CMV and T7 binary promoter, NSP1-4 replicon protein genes from VEE virus under the control of the binary promoter, a 26S sub-genomic promoter from the VEE virus, multi-cloning site for insertion of a nucleic acid encoding a payload under the control of the sub-genomic promoter and optionally resistance genes for puromycin (PuroR) and ampicillin (AmpR) for mammalian and bacterial cell culture, respectively.

[0060] In certain embodiments, the nucleic acid vector lacks a ribsosome binding site RBS necessary for the initiation of translation in bacteria, such as Salmonella. In such embodiments, the DNA and mRNA carried by the bacteria will only be translated (e.g. protein expression) after bactofection (uptake of bacteria by invasion / phagocytosis / infection) of the eukaryotic cells. In alternative embodiments, the vectors comprise Kozak and RBS for universal protein expression in prokaryotic and eukaryotic cells.

[0061] In specific embodiments, the vector comprises the sequence of any of the vectors set in the application. A worker skilled in the art would readily appreciate that the GFP and the Spike sequences are non-limiting exemplary payload sequences which may be replaced with other payload sequences.

[0062] The bacteria comprising the self-amplifying vectors of the present invention may be utilized to express a variety of payloads, including one or more nucleic acids, one or more peptides and one or more polypeptides.

[0063] In certain embodiments, the payload is one or more RNAs including microRNAs and other functional mRNAs.

[0064] In certain embodiments, the payload is one or more polypeptides. The polypeptide(s) may be any peptide or polypeptide. Exemplary peptides and polypeptides include but not limited to immunogens; epitopes; antibodies, SFv; immunomodulatory molecules including but not limited to cytokines; growth factors; fusion proteins; suicide proteins; CRISPR CAS9 or other recombinase system, proteins that enhance infectivity of the bacteria, such as invasion, antimicrobial peptides and any other proteins. The immunogens and / or epitopes may be from an infectious agents including but not limited to viruses such as coronaviruses and influenza, bacteria or fungi; or cancer specific immunogens and / or eptiopes.

[0065] In certain embodiments, the vector further comprises a suicide construct to turn off the vector under the control of an inducible promoters.

[0066] The present invention further comprises pharmaceutical compositions including vaccine formulations comprising the bacteria comprising the nucleic acid vectors of the present invention alone or in combination with one or more pharmaceutically acceptable carriers or diluents and / or one or more additional therapeutic agents.

[0067] The pharmaceutical composition may be formulated for administration by various routes including but not limited to intramuscular, intradermal, subcutaneous, intranasal, oral, Intravenous, intraperitoneal, sublingual, topical, inhalation, suppository, intraosseous, and intraarticular. In certain embodiments, the pharmaceutical composition are formulated for oral administration. In such embodiments, the compositions may comprise a carrier useful for protecting the microorganism from the stomach acid or other chemicals, such as chlorine from tap water, that may be present at the time of administration. For example, the bacteria may be administered as a suspension in a solution containing sodium bicarbonate and ascorbic acid (plus aspartame as sweetener).

[0068] Suitable formulations for oral administration include hard or soft gelatin capsules, pills, tablets, including coated tablets, elixirs, suspensions, syrups or inhalations and controlled release forms thereof. Gelatin capsules can serve as carriers for lypholized vaccines.

[0069] In certain embodiments, the dosage of bacteria comprising the vector for administration is 1.0x1 o3to 1.0x1 o15CFU / ml or cells / ml. For instance, the invention includes a vaccine with about 1 .0X 103, about 1 .0X104, about 1 .0X105, about 1 .0X106, about 1 .0X107, about 1 .0X108, about 1 .0X109, about 1.0xl01°, about 1.0x1011, about 1 .0X1012, about 1.0x1013, about 1 .0X1014, about 1 ,0x1015CFU / ml or cells / ml. In certain embodiments, the dosage administered will be dependent upon the age, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired.

[0070] In certain embodiments, the compositions of this invention may be co-administered along with other compounds, including other therapeutic agents. Such other therapeutic agents may include but are not limited to immunostimulatory agents.

[0071] A vaccine adjuvant is a component that potentiates the immune responses to an antigen and / or modulates it towards the desired immune responses. A vaccine may include one or more adjuvants. Exemplary adjuvants include mineral salts including but not limited to aluminium salts (such as amorphous aluminum hydroxyphosphate sulfate (AAHS), aluminum hydroxide, aluminum phosphate, potassium aluminum sulfate (Alum)) and calcium phosphate gels; Oil emulsions and surfactant based formulations, including but not limited to MF59, QS21 (purified saponin), AS02 [SBAS2] (oil-in-water emulsion + MPL + QS-21), Montanide ISA-51 and ISA-720 (immunoprec water-in-oil emulsion); Particulate adjuvants, including but not limited to virosomes (unilamellar liposomal vehicles incorporating influenza haemagglutinin), AS04 ([SBAS4] Al salt with MPL), ISCOMS (structured complex of saponins and lipids), polylactide co-glycolide (PLG). And ; microbial derivatives (natural and synthetic), including but not limited to monophosphoryl lipid A (MPL), Detox (MPL + M. Phlei cell wall skeleton), AGP [RC-529] (synthetic acylated monosaccharide), DC_Chol (lipoidal immunostimulators), OM-174 (lipid A derivative), CpG motifs (synthetic oligonucleotides containing immunostimulatory CpG motifs), modified LT and CT (genetically modified bacterial toxins to provide non-toxic adjuvant effects); endogenous human immunomodulators, including but not limited to hGM-CSF or h IL- 12 (cytokines that can be administered either as protein or plasmid encoded), Immudaptin (C3d tandem array) and inert vehicles, such as gold particles.

[0072] The pharmaceutical compositions and vaccine formulations may also comprise a stabilizer. Suitable stabilizers are known in the art and include but are not limited to amino acids, antioxidants, cyclodextrins, proteins, sugars / sugar alcohols, and surfactants. See for example Morefield, AAPS J. 2011 Jun; 13(2): 191-200; https: / / www.ncbi.nlm.nih.qov / pmc / articles / PMC3085699 / ).

[0073] A prime-boost protocol is often a suitable method of administering vaccines. In a prime-boost protocol, one or more compositions of the present invention can be utilized in a “prime boost” regimen.

[0074] Methods of Use

[0075] The present invention further provides a method of delivering a payload of interest to an eukaryotic cell, the method comprising contacting the cell (either in vitro or in vivo) with bacteria, optionally Salmonella comprising the self-amplifying vector of the present invention which expresses the payload. The cell may be a mammalian cell. In certain embodiments, expression of the payload prevents, delays and / or treats disease.

[0076] The bacteria, optionally Salmonella comprising the self-amplifying vector of the present invention may be administered to a variety of subjects. In certain embodiments, the vector the subject is a human or other animals, including but not limited to other mammals, such as non-human primates, cats, dogs, equines (including but not limited to horses, donkeys and zebras), camels, sheep, goats, and bovines (including but not limited to cows).

[0077] In certain embodiments, the bacteria, optionally Salmonella comprising the selfamplifying vector of the present invention are used as a vaccine. Accordingly, also provided herein is a method of treating, protecting against, and / or preventing disease associated with the infectious agent in a subject in need thereof by administering the vaccine to the subject. For example, a worker skilled in the art would readily appreciate that a SARS-CoV-2 vaccine may be used for treating, protecting against, and / or preventing disease associated with SARS-CoV-2 (i.e. COVID 19). Likewise, an influenza vaccine may be used for treating, protecting against, and / or preventing disease associated with influenza. Administration of the vaccine to the subject can induce or elicit a specific immune response against the vaccine target in the subject.

[0078] In certain embodiments, the bacteria, optionally Salmonella comprising the selfamplifying vector of the present invention are used as a vaccine against cancer. In such embodiments, the vectors express one or more cancer antigens or eptiopes alone or in combination with other therapeutic proteins such as immunostimulatory molecules.

[0079] The induced immune response can be used to treat, prevent, and / or protect against disease related to the vaccine target. For example, a SARS-CoV-2 vaccine to the subject can induce or elicit a specific immune response against the SARS-CoV-2 virus in the subject. The induced immune response provides the subject administered the vaccine with protection against the vaccine target, such as a SARS-CoV-2 vaccine provides resistance to SARS-CoV-2.

[0080] The induced immune response can include an induced humoral immune response and / or an induced cellular immune response. The induced humoral immune response can include IgG antibodies and / or neutralizing antibodies that are reactive to the antigen. The induced cellular immune response can include a CD8+ T cell response. The number of vaccine doses for effective treatment can be 1 , 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0081] The bacteria, optionally Salmonella comprising the self-amplifying vector of the present invention can be administered prophylactically or therapeutically.

[0082] The bacteria, optionally Salmonella comprising the self-amplifying vector of the present invention may be used in imaging. For example, the vector may express a fluorescent protein. The bacteria, optionally Salmonella comprising the self-amplifying vector of the present invention may be used alone or in combination with other agents.

[0083] To gain a better understanding of the invention described herein, the following examples are set forth. It will be understood that these examples are intended to describe illustrative embodiments of the invention and are not intended to limit the scope of the invention in any way.

[0084] EXAMPLES

[0085] In recent years, the development of vaccines using novel platforms such as mRNA and adenovirus vectors has garnered significant attention due to their potential for rapid development and deployment. However, Salmonella-based expression platforms offer several distinct advantages over these conventional approaches. Salmonella-based expression platforms have demonstrated high efficacy in inducing robust immune responses against a wide range of pathogens. Unlike mRNA vaccines, which rely on the delivery of nucleic acids to host cells for protein expression, Salmonella vectors can efficiently deliver antigens directly to the host's immune system. This direct antigen presentation results in potent immune activation, including both humoral and cellular responses (Curtiss & Xin, 2014). Moreover, Salmonella vectors have been shown to induce long-lasting immunity, providing durable protection against infectious diseases. This is attributed to the ability of Salmonella to persistently colonize the host's mucosal surfaces, allowing for continuous antigen exposure and immune stimulation (Forbes & Forbes, 2010). Salmonella-based expression platforms offer unparalleled versatility in vaccine design and antigen delivery. Salmonella vectors can accommodate a wide range of antigens, including complex proteins and multi-epitope constructs, making them ideal for the development of polyvalent vaccines targeting multiple pathogens or antigenic variants (Curtiss & Xin, 2014). Additionally, Salmonella vectors can be engineered to express antigens from different pathogens, allowing for the development of combination vaccines against co-endemic diseases. This flexibility enables researchers to tailor vaccine formulations to specific epidemiological contexts and public health needs (Mastroeni & Grant, 2011). One of the most significant advantages of Salmonella-mediated antibody expression is its potential for oral delivery, offering a non-invasive and cost-effective route of administration. Salmonella vectors can survive passage through the acidic environment of the stomach and colonize the host's intestinal mucosa, where they efficiently express and secrete antibodies (Minton et al., 2016). Oral delivery of antibodies via Salmonella vectors mimics natural immune responses, stimulating both mucosal and systemic immunity. This mucosal immunity is particularly important for diseases affecting mucosal surfaces, such as gastrointestinal infections and respiratory diseases. Moreover, Salmonella-mediated oral delivery enables repeated administration of antibodies without the need for injections or infusions, improving patient compliance and accessibility (Lalsiamthara et al., 2017). Salmonella-based expression platforms offer numerous advantages over mRNA and adenovirus-based vaccines, including high efficacy, versatility, and potential for oral delivery. These platforms represent promising tools for vaccine development, with the potential to address current challenges in global health, such as infectious disease outbreaks and emerging pathogens.

[0086] In this Example, the possibility of using an attenuated strain of Salmonella (SL1344) as a vaccine or therapeutic drug was explored. Salmonella was used as a carrier for self-amplifying expression vectors that encode all or a portion of replicon proteins from a positive stranded virus (WO2022 / 246559 and PCT / CA2023 / 051444 herein incorporated by reference). The insertion of prokaryotic ribosomal binding site into the construct allows the salmonella to express the recombinant proteins and mRNA inside of salmonella already and we expect antigenic proteins to be processed for the prompt antigen presentation or replicon proteins required for the self replication from APC right after the process called bactofection without any significant delay associated with the transport of transfected plasmid DNA from / to cytoplasm from / to nucleus for transcription and translation. Lac promoter or T7 promoter can be used as a prokaryotic specific promoter. Lac promoter can be used to accomplish the inducible system expressing the cytotoxic recombinant protein if necessary. The system with dual promoters and dual ribosomal binding sites in theory allows it to express vaccine proteins or therapeutic drugs in bacteria and mammalian cells consistently. As a result Salmonella can be such a good and promising DNA vaccine carrier because Salmonella has LPS and flagella on the membrane or from a thin peptidoglycan wall respectively activating TLR4 and TLR5 signalling pathways respectively and in turn the innate immunity. Salmonella based gene delivery platform may provide the immunized people with additional protection by more virulent salmonella infections as well.

[0087] Methods: In this study, three different Salmonella-based platforms will be used: attenuated strains, plasmid-based vectors, and recombinant protein expression systems. For gene expression analysis, quantitative PCR will be used to measure the transcription levels of recombinant genes. Western blot analysis will be used to assess protein expression levels in bacterial lysates. Immunofluorescence staining and flow cytometry will be utilized to visualize and quantify protein expression in host cells. Vaccine efficacy will be evaluated by administering orally vaccinated mice with virulent Salmonella challenge strains. Bacterial burden, survival rates, and clinical symptoms will be monitored to assess vaccine protection. Immune responses will be characterized by measuring B cell antibody responses, neutralizing antibodies, cytokine responses, and T cell memory responses using ELISA, plaque reduction assays, ELISPOT assays, and flow cytometry.

[0088] Preparation of electrocompetent attenuated strain of Salmonella, SL1344: https: / / www.nature.com / articles / s42003-020-1Q72-4

[0089] Making electrocompetent cells:

[0090] 1. Inoculate a flask of LB 1 :100 with a fresh overnight culture, grow at 37 °C with shaking to an O.D.600 of 0.75. Chill the cells in an ice bath for 15 min.

[0091] 2. Harvest by centrifugation (4,000 x g, 10 min., 4 °C).

[0092] 3. Wash one time in the original culture volume with HEPES and one time with 10% glycerol using 1 / 100 of the original volume.

[0093] 4. Resuspend in 10% glycerol.

[0094] Transformation by electroporation with Self-amplifying DNA vector expressing with Cov-2 omicron spike or eGFP

[0095] Electroporation of Salmonella cells:

[0096] 1 . Add 2 pl plasmid DNA (20 pg to 20 ng) to 50 pl of electrocompetent bacterial cells. Homogenize by gently mixing with pipette several times. Transfer mixture into a prechilled cuvette (0.2cm gap).

[0097] 2. Wipe moisture from the cuvette and insert the cuvette into the device.

[0098] 3. Electroporation parameters applied: 2.5kV, 25pF, and 200Q 4. Immediately add 1 ml SOC medium and transfer to a sterile culture tube. Incubate for 1 hour at 37 °C with shaking at 200 rpm.

[0099] 5. Dilute the cells in SOC medium and plate about 50ul of electroporated cells on selective LB plates with the antibiotic of choice (e.g. ampicillin or kanamycin).

[0100] Assessment of replication, transcription and translation (Protein expression) of Gemini vectors in transformed Salmonella.

[0101] Replication of plasmid in the transformers cells can simply be assessed by performing miniprep of plasmid DNA (see: https: / / www.ncbi.nlm.nih.qov / pmc / articles / PMC4972323 / a. Resuspend pellet in 200 pl Plasmid Resuspension Buffer containing the RNaseA. Vortex to ensure cells are completely resuspended. There should be no visible clumps. b. Lyse cells by adding 200 pl Plasmid Lysis Buffer. Gently invert the tube 20 times. Do not vortex! Incubate for 5 minutes. c. Clarify the lysate by spinning for 10 minutes at 16,000 x g. d. Carefully transfer supernatant to the spin column and centrifuge for 1 minute. Discard flow-through. e. Re-insert column in the collection tube and add 200 pl of Plasmid Wash Buffer 1 . Centrifuge for 1 minute and discarding the flow-through f. Add 400 pl of Plasmid Wash Buffer 2 and centrifuge for 1 minute. g. T ransfer column to a clean 1 .5 ml microfuge tube. h. Re-spin the column for 1 minute before inserting it into the clean microfuge tube. i. Add 50pl DNA Elution Buffer to the center of the matrix. Wait for 1 minute, then spin for 1 minute to elute DNA.

[0102] Transcription from the transformed pDNA can be assessed by performing RT-PCR. Traditionally RT-PCR involves two steps: the RT reaction and PCR amplification. RNA is first reverse transcribed into cDNA using a reverse transcriptase, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes in the pDNA like cov-2 spike or eGFP. RNA extraction: Total RNA is extracted from transformed salmonella cells using a standard RNA extraction spin column and dissolved in RNase free water and quantified with the nanodrop at the wavelength of 260 nm.

[0103] Reverse transcription (conversion from RNA to cDNA): The present invention relates to an expression vector that encodes all or a portion of replicon proteins from a positive stranded virus, wherein expression of the replicon proteins is under the control of CMV and T7 promoters, and wherein expression of a payload is under the control of a sub-genomic promoter. Also provided are methods of using the vector in therapeutics and vaccines.

[0104] RT-PCR is used to clone expressed genes by reverse transcribing the RNA of interest into its DNA complement through the use of reverse transcriptase. Subsequently, the newly synthesized cDNA is amplified using traditional PCR. The total RNA is used as a template and Oligo (dT) or random primers and reverse transcriptase are used to convert transcribed mRNA into cDNA.

[0105] PCR amplification

[0106] • Thaw all reagents on ice.

[0107] • Assemble reaction mix into 50 pL volume in a thin walled 0.2 mL PCR tubes.

[0108] • Add reagents in following order: water, 2X PCR master mix, template, and primers.

[0109] • Gently mix by tapping tube. Briefly centrifuge to settle tube contents.

[0110] • Prepare negative control reaction without template DNA.

[0111] • Prepare positive control reaction with template of known size and appropriate primers.

[0112] • Program your thermocycler for your PCR reaction

[0113] • Analyze the results of your PCR reaction via gel electrophoresis on 1% agarose gel

[0114] Translation of the plasmid DNA via transcription can be assessed by performing the western blots using total cell lysates from the transformed bacteria

[0115] Preparation of lysate from cell culture: Place the bacterial cell culture tube on ice, quickly spin the bacterial cells and wash the cells with ice-cold PBS. Aspirate the PBS, then add an ice-cold lysis buffer and incubate them for 10 min with occasional mixing by inverting tubes (constant agitation for 30 min at 4°C). Centrifuge the cell suspension in a microcentrifuge at 4°C. Spin the cells for 5 min and gently transfer the cell suspension into a pre-cooled microcentrifuge tube. Discard the pellet.

[0116] Sample preparation: Remove a small volume of lysate to perform a protein quantification assay. Determine the protein concentration for each cell lysate.

[0117] Determine how much protein to load and add an equal volume 2X Laemmli sample buffer. To reduce and denature your samples, boil each cell lysate in the sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

[0118] Loading and running the gel: Load equal amounts of protein into the wells of the SDS-PAGE gel, along with a molecular weight marker. Load 20-30 pg of total protein from cell lysate or tissue homogenate, or 10-100 ng of purified protein. Run the gel for 1-2 h at 100 V.

[0119] Transferring the protein from the gel to the membrane: The membrane can be either nitrocellulose or PVDF. Activate PVDF with methanol for 1 min and rinse with transfer buffer before preparing the stack. The time and voltage of transfer may require some optimization. We recommend following the manufacturer’s instructions. Transfer of proteins to the membrane can be checked using Ponceau S staining before the blocking step.

[0120] Antibody staining: Block the membrane for 1 h at room temperature or overnight at 4°C using blocking buffer. Incubate the membrane with appropriate dilutions of primary antibody in blocking buffer. Overnight incubation at 4°C. Wash the membrane in three washes of PBST, 5 min each. Incubate the membrane with the recommended dilution of conjugated secondary antibody in blocking buffer at room temperature for 1 h. Wash the membrane in three washes of PBST, 5 min each. For signal development, follow the kit manufacturer’s recommendations. Acquire image by performing chemiluminescence based detection method.

[0121] RAW cell in vitro study https: / / www.ncbi.nlm.nih.qov / pmc / articles / PMC6760541 / To assess the ability of the attenuated Salmonella strain (SL1344) to transfect murine macrophage cell line (RAW 264.7) in tissue culture (it is called ‘Bactofection’), in vitro challenge study was performed using Gemini 1.0 expressing eGFP for easy and quick evaluation (green fluorescence from the bactofected RAW264.7 cells). This bactofection by the attenuated salmonella into the various antigen presenting and immune cells in the gut are most critical to the onset of humoral and cellular immunity from vaccines in vivo.

[0122] Cell culture: RAW264.7 macrophages (from ATCC) were split, grown and maintained in DMEM with 10% FBS. Cells were plated onto six-well plates at a plating density of 4 x 105 per well in a total of 3 ml of media 1 day prior to the bactofection.

[0123] Challenge studies: Bacteria from frozen stocks were streaked onto fresh LB agar plates and incubated at 37°C overnight. A single bacterial colony was inoculated into LB broth and incubated in a shaking incubator overnight at 37°C. A 1 : 10 dilution of the overnight culture was further incubated with shaking for 2 h at 37°C. Bacteria were centrifuged at 4300 xg for 10 min, washed in cold PBS and spun twice, and resuspended into an equal volume of phosphate-buffered saline (PBS). Optical density at 600 nm (O.D.600) was measured to determine the bacterial number count, and the inoculum was diluted in PBS (200 pl final volume) to achieve the correct MOI (Salmonella:RAW cells=50: 1 ). RAW 264.7 cells were then infected with 50 MOI of S. enterica in 2% FBS containing DMEM for 3 h. After incubation, the unbound bacteria were washed three times with cold PBS. Growth medium with 10% FBS was then added to the cells with Genetamycin to kill off uninfected Salmonella and cells were grown for 2 additional days until harvesting cells for assays.

[0124] Oral gavage methods: https: / / www.ncbi.nlm.nih.qov / pmc / articles / PMC6687127 / #:~:text=Pretreatment%20of %20C57BI%2F6%20mice,stomach%20via%20a%20feedinq%20needle.

[0125] Except after a meal, when the stomach is full of food, the pH of the normal human stomach can be very low, around pH 2, which will kill a lot of Salmonella and many other bacteria that cause enteric diseases. Therefore, 30 min prior to the oral infection by salmonella, mice were treated with 10% sodium bicarbonate and subsequently 107SL1344 salmonella cells were subsequently injected per mouse using a gavage needle (orally). SARS-CoV-2 spike ELISA protocol: SARS-CoV-2 RBD of spike protein (Proteogenix, Strain B1.1.529, PX-COV-P074) for the Omicron strain was diluted to 100 ng / mL (low antigen concentration) or 1 .0 pg / ml (high antigen concentration) and coated onto 96 well plates using coating buffer (0.1 M Carbonate, pH 9.5). After overnight incubation at 4 °C, plates were washed four times with washing buffer (0.1% Tween-20 in 1X PBS). Subsequently, plates were blocked with blocking buffer (2% BSA, 0.1% Tween-20 in 1X PBS) overnight at 4 °C then washed five times with washing buffer. Serum samples from mice immunized with the SARS-CoV-2 virus Omicron (B.1.1.529) spike protein was serially diluted 3-fold in blocking buffer from 1 :240 to 1 :2160. In each well, 100 pL of serum sample dilutions were added and plates were incubated away from light at 37 °C for 1 hour. Plates were then washed four times with washing buffer before incubating with 100 pL Goat anti-mouse HRP-conjugated secondary antibody (Southern Biotech, 1030-05; 1 :4000 dilution in blocking buffer) at 37 °C for 1 hour. Plates were finally washed with washing buffer five times before adding 100 pl / well of TMB substrate (ThermoFisher Scientific, 34028) and incubated away from light at room temperature for 20 minutes to allow for colour development. Reaction was stopped by adding 100 pl / well of stopping solution (0.16 N H2SO4). Chemiluminescence of the plates were read using ELISA plate reader at 450 nm. For Omicron spike protein, positive serum was made in house by vaccinating mice with Omicron spike protein in alum adjuvant. Quantified antibody from positive control serum was used to set up standard curve using GraphPad prism (Version 10.0.1) from which the unknown antibody values were interpolated and the results expressed in ng / ml.

[0126] Fecal Sample processing: 100 mg of feces were soaked in 1 ml of PBS at room temperature for 10-12 hours. After 10-12 hours of wetting the fecal pellets were vortexed until fecal pellets were dissolved. The samples were then centrifuged at 13,000g / 10 minutes / 4°C. The resulting supernatant was collected and stored at - 20°C until further use.

[0127] Pseudotype virus neutralization assay: A luciferase-reporter based system was used to assess the neutralization ability of sera from vaccinated animals against SARS- CoV. For the neutralization assay, heat-inactivated sera were first serially diluted and incubated with virus for 1 h, and then the serum-virus mixture was transferred into wells pre-seeded HEK-293 cells. After 48 h, cells were lysed, and luciferase activity was measured. The IC5o neutralization was then calculated. The pseudovirus neutralization data titration were obtained based on the standard curve.

[0128] ELISPOT as a measure of T cell immunity

[0129] The T cells from the vaccinated mice are incubated with the antigen presenting cells which are pulsed with peptides from the vaccine. Primed T cells from the vaccinated mice will then proliferate in response to the peptides. The amount of proliferation of T cells is proportionate to the population of primed T cells in the spleens. T cells in response to proliferation release interferon-gamma which is a key pro-inflammatory cytokine. This cytokine is captured on the plate and the readout are the number of interferon-gamma spots produced as a result. The number of interferon-gamma spots are then a measure of the number of primed T cells in the spleen cells and is also an indication of good vaccination. Good vaccine induces higher number of spots compared to the poor vaccine which induces lower number of spots

[0130] Experimental Protocol: In brief, spleens from the vaccinated mice were harvested at the end of the experiment. Spleen cells (containing T cells) were then counted and plated at 5x105 cells / well in an Enzyme Linked Immunospot assay (ELISPOT) plate For antigen presentation, DC 2.4 cells (Dendritic cells- professional antigen presenting cells) were then added which were pulsed with overlapping peptides for Spike protein of SARS Cov-2. The cells were then incubated for 48 hours in the incubator with 5% CO2.

[0131] Results:

[0132] • Salmonella mode of vaccine delivery induces robust B and T cell responses to target antigen.

[0133] • There is no antibody response against the proteins that comprise the VEEV replicon of the vector. This may be because all the replicon proteins are intracellular and never exposed for generating antibody responses.

[0134] • There is no anii-Salmonella response generated when administering orally. This may be because of tolerance to orally given enteric gram negative bacteria because of the subject’s constituent microbiomes. The lack of antibody response to the VEEV replicon and lack of anii-Salmonella response suggests that this approach may be used multiple times on a subject.

[0135] • Very high titer of antibody (up to 600 micrograms / ml) is induced.

[0136] • Strong mucosal immunity is induced as well. • Strong T cell immunity is induced as evident by Peripheral Blood Mononuclear Cell (PBMC) assay and Enyzme Linked Immunospot (ELISPOT) assay.

[0137] • A dose titration with higher doses (5x107cfu and 5x108cfu) of Salmonella corresponding to increased response.

[0138] SEQUENCES

[0139] SEQ ID NO: 1 : DNA sequence of the pDA with codon optimized omicron spike glycoprotein and both prokaryotic (underline) and eukaryotic ribosomal sites (bolded) constructed in Gemini 1.0 VEE SA replicon proteins

[0140] AATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATA GCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGA GGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCG TGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGAT GTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTATAGTCGTCA ACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCGTAGTGGCC CAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACT AGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGA TAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCAAAGTGAACAGCGATTTCC ACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAGATCGGGCTGAGAACAAGA ATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGT ACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTG CGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAGGCAGACG TCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCTTGATA AAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTGCTTTCTCCGCA GGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGT GATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAG TGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCC ACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACCTGCACCATATTGCCACACAT GGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGA CGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTG GGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAG TCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTGC CAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCACCAAAAAAGATCTAGTGGTG AGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAGGGCT GGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAG AGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAG CCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGATCCCAAACAGTGCGGTTTTTTTA ACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCACACAAGTCTTCCACAAAA GCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACA AAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGT ACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTT GCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACCC GTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCACCCACC TCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACT AGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCA CGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACC GGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGTTGGGCCAAGGCTTTAGTGC CGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTGTGGATTAT TTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTC TTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGG AATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGT CCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGAGTCT ATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTACCTGTAA

[0141] ACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTT

[0142] CTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCC

[0143] GTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCG

[0144] GCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACATAATATTTGTTAATGTGAG

[0145] GACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTAGCAT

[0146] GTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTT

[0147] ATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAG

[0148] TTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATTC

[0149] ATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAACCTTGACC

[0150] AACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGGTG

[0151] CGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGG

[0152] ACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGAT

[0153] TTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAAAGGTGCAGCTAAACATATCAT

[0154] TCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTGACAAACAGTTGG

[0155] CAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGA

[0156] TTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGA

[0157] ACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACA

[0158] AGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGAT

[0159] ATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGGGTGCATC

[0160] CGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCA

[0161] TATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCAT

[0162] GTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCATGTATATCCTCGGAGAAAGC

[0163] ATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGGAAGCCTCCACACCACCTAG

[0164] CACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAAAAG

[0165] CCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCA

[0166] CTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCG

[0167] TATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCC

[0168] ATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGAGG

[0169] ATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAG

[0170] CATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTC

[0171] ACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATG

[0172] TGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTAGCGTGACCAGCGGGGCAAC

[0173] GTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGCGACCGGTGC

[0174] CTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCG

[0175] TCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCG

[0176] TGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAG

[0177] CAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATT

[0178] ACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTGC

[0179] ATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAAC

[0180] GGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGC

[0181] CTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACAGTTAAATCCCACACCTGCT

[0182] AACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCATAACAGCTAGACG

[0183] TATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAA

[0184] CCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGG

[0185] TCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACT

[0186] GTATTATTCCAGAGTACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAG

[0187] ACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGG

[0188] AACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTG

[0189] GCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGA

[0190] TTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGA

[0191] AACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAACGTGGTAAATTACATTACCAA

[0192] ATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGAATATGTTGCA

[0193] GGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAG

[0194] GAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTA

[0195] GCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCT

[0196] GCTTCCGAACATTCATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGC

[0197] CGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAA

[0198] GTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGAC GCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCC

[0199] CACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTTCCTCACACTGTTT

[0200] GTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTGAGAGAACGGCTAACCGG

[0201] ATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGGACAA

[0202] ATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGT

[0203] GGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCG

[0204] GCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGGCTGTTTAAGCTTGGCAAACCTCTG

[0205] GCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACAC

[0206] GCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAA

[0207] ACCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCA

[0208] TTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGAC

[0209] ATAGTCTAGTCCGCCAAGTCTGTTTAAACAGCATATGGGCGCGCCCTCAGCATCGATTC

[0210] AATTCAAGGAGATTGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGC

[0211] CAGTGCGTGAACCTTACAACCAGGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCAC

[0212] AAGGGGCGTTTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAG

[0213] GACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAA

[0214] CGGCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCC

[0215] AGCATCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCA

[0216] AGACCCAGAGCCTTCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGA

[0217] GTTCCAGTTCTGCAACGACCCCTTCCTGGACCACAAGAACAACAAGAGCTGGATGGAGA

[0218] GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCC

[0219] CTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTG

[0220] TTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCTATCATCGTGAG

[0221] GGAGCCCGAGGACCTGCCTCAGGGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCC

[0222] ATCGGCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

[0223] ACCTGGAGACAGCAGCTCTGGCTGGACAGCTGGAGCCGCTGCTTACTACGTGGGCTAC

[0224] CTGCAACCTAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGT

[0225] GGACTGCGCCCTTGATCCTCTGTCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGG

[0226] AGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAG

[0227] GTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCG

[0228] CCAGCGTGTACGCTTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGT

[0229] GCTGTACAACCTGGCCCCTTTCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGC

[0230] TGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAA

[0231] GTGAGGCAGATCGCCCCTGGCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGC

[0232] CCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGT

[0233] GAGCGGCAACTATAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

[0234] AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGC

[0235] CGGCTTCAACTGCTACTTCCCCTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTG

[0236] GACACCAGCCTTACAGGGTGGTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCAC

[0237] CGTTTGCGGACCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACT

[0238] TCAATGGCCTGAAGGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTT

[0239] CCAGCAGTTCGGCAGGGACATCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACA

[0240] CTGGAGATCCTGGACATCACCCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTG

[0241] GCACCAACACAAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGT

[0242] GCCTGTTGCCATCCACGCCGACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGC

[0243] AGCAACGTTTTCCAGACAAGGGCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACA

[0244] GCTACGAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGAC

[0245] CAAGAGCCACAGCGTGGCCAGCCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCC

[0246] GAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAG

[0247] CGTGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATG

[0248] TACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCT

[0249] GCACCCAGCTGAAGAGGGCCCTGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCA

[0250] GGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGTACTTCGGCG

[0251] GCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATC

[0252] GAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

[0253] GAGACTGCTTGGGAGACATCGCCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGG

[0254] CCTGACCGTGTTGCCTCCCCTGCTGACCGACGAGATGATCGCTCAGTACACCAGCGCC

[0255] CTGTTGGCCGGCACCATTACATCTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGAT

[0256] CCCCTTTGCCATGCAGATGGCTTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGC TGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAG

[0257] GATAGCCTGAGCAGCACCGCTTCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACA

[0258] ACGCCCAGGCCCTTAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAG

[0259] CAGCGTGCTGAACGACATCTTCAGCAGGCTGGACccccctGAGGCCGAGGTGCAGATCGA

[0260] CAGGCTGATCACAGGCAGGCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATT

[0261] AGGGCCGCCGAGATCAGGGCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCG

[0262] TGCTGGGCCAGAGCAAGAGAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTT

[0263] CCCTCAGAGCGCCCCTCATGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAG

[0264] GAGAAGAACTTCACCACCGCCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAG

[0265] GGAGGGAGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTAC

[0266] GAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGA

[0267] TCGGCATCGTGAACAACACCGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAA

[0268] GGAGGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGAC

[0269] ATCAGCGGAATTAACGCTAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGA

[0270] GGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

[0271] CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCAT

[0272] CGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGC

[0273] TGCTGCTCTTGTGGCAGCTGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGA

[0274] AGGGCGTGAAGCTGCACTACACCtagTCTAGAGTGTTTAAACCGACCCGGGCGGCCGCA

[0275] ACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTA

[0276] ACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTT

[0277] CCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTG

[0278] ACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGT

[0279] CGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCC

[0280] TTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACG

[0281] TGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAG

[0282] TTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCC

[0283] CAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATG

[0284] TGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCC

[0285] TTTGAAAAACACGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCG

[0286] CGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGC

[0287] CACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAA

[0288] CTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGC

[0289] GCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGC

[0290] CGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACA

[0291] GATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCAC

[0292] CGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCC

[0293] CGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGC

[0294] CCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGT

[0295] GCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCA

[0296] AGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTT

[0297] TCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAA

[0298] AACGCGTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGG

[0299] AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC

[0300] ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTC

[0301] AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCA

[0302] CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTT

[0303] ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT

[0304] TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGAC

[0305] GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTA

[0306] CTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG

[0307] CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA

[0308] CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG

[0309] TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT

[0310] GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC

[0311] CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTC

[0312] GGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTC

[0313] GCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC

[0314] ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA

[0315] TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGA

[0316] CCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCA

[0317] AAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC

[0318] ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGG

[0319] TAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG

[0320] GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC

[0321] CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG

[0322] TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT

[0323] TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGC

[0324] CACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAAC

[0325] AGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTC

[0326] GGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGA

[0327] GCCTATGGAAAAACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAACGTTACATAACTT

[0328] ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAA

[0329] TGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGT

[0330] ATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCC

[0331] CTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTAT

[0332] GGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGC

[0333] GGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGT

[0334] CTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCA

[0335] AAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGG

[0336] AGGTCTATATAAGCAGAGCTTAATACGACTCACTATAGGGCCGGCCATGGGCGGCGCAT

[0337] GAGAGAAGCCCAGACCAATTACCTAAAGGAGATTCCCAAAATGGAGAAAGTTCACGTTG

[0338] ACATCGAGGAAGACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAG

[0339] GTAGAAGCCAAGCAGGTCACTGATAATGACCATGCTAATGCCAGAGCGTTTTCGCATCT

[0340] GGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGATCCTTGACATTGGAA

[0341] GTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT

[0342] GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAG

[0343] GAAATAACTGATAAGGAATTGGACAAGAAAATGAAGGAGCTGGCCGCCGTCATGAGCGA

[0344] CCCTGACCTGGAAACTGAGACTATGTGCCTCCACGACGACGAGTCGTGTCGCTACGAAG

[0345] GGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAAGTCTCTATCAC

[0346] CAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTAT

[0347] GTTTAAGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCG

[0348] TGTTAACGGCTCGTAACATAGGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGA

[0349] GGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTG

[0350] GCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGT

[0351] ATTTCACTTACGTGGCAAGCAA

[0352] SEQ ID NO: 2: CMV+T7_Codon optimized omicron spike with Kozak only (original)

[0353] AATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATA

[0354] GCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGA

[0355] GGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCG

[0356] TGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGAT

[0357] GTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTATAGTCGTCA

[0358] ACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCGTAGTGGCC

[0359] CAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACT

[0360] AGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGA

[0361] TAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCAAAGTGAACAGCGATTTCC

[0362] ACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAGATCGGGCTGAGAACAAGA

[0363] ATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGT

[0364] ACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTG

[0365] CGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAGGCAGACG

[0366] TCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCTTGATA

[0367] AAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTGCTTTCTCCGCA

[0368] GGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGT

[0369] GATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAG

[0370] TGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCC ACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACCTGCACCATATTGCCACACAT

[0371] GGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGA

[0372] CGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTG

[0373] GGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAG

[0374] TCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTGC

[0375] CAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCACCAAAAAAGATCTAGTGGTG

[0376] AGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAGGGCT

[0377] GGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAG

[0378] AGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAG

[0379] CCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGATCCCAAACAGTGCGGTTTTTTTA

[0380] ACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCACACAAGTCTTCCACAAAA

[0381] GCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACA

[0382] AAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGT

[0383] ACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTT

[0384] GCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACCC

[0385] GTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCACCCACC

[0386] TCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACT

[0387] AGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCA

[0388] CGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACC

[0389] GGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGTTGGGCCAAGGCTTTAGTGC

[0390] CGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTGTGGATTAT

[0391] TTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTC

[0392] TTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGG

[0393] AATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGT

[0394] CCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGAGTCT

[0395] ATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTACCTGTAA

[0396] ACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTT

[0397] CTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCC

[0398] GTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCG

[0399] GCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACATAATATTTGTTAATGTGAG

[0400] GACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTAGCAT

[0401] GTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTT

[0402] ATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAG

[0403] TTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATTC

[0404] ATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAACCTTGACC

[0405] AACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGGTG

[0406] CGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGG

[0407] ACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGAT

[0408] TTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAAAGGTGCAGCTAAACATATCAT

[0409] TCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTGACAAACAGTTGG

[0410] CAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGA

[0411] TTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGA

[0412] ACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACA

[0413] AGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGAT

[0414] ATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGGGTGCATC

[0415] CGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCA

[0416] TATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCAT

[0417] GTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCATGTATATCCTCGGAGAAAGC

[0418] ATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGGAAGCCTCCACACCACCTAG

[0419] CACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAAAAG

[0420] CCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCA

[0421] CTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCG

[0422] TATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCC

[0423] ATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGAGG

[0424] ATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAG

[0425] CATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTC

[0426] ACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATG

[0427] TGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTAGCGTGACCAGCGGGGCAAC

[0428] GTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGCGACCGGTGC CTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCG

[0429] TCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCG

[0430] TGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAG

[0431] CAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATT

[0432] ACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTGC

[0433] ATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAAC

[0434] GGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGC

[0435] CTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACAGTTAAATCCCACACCTGCT

[0436] AACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCATAACAGCTAGACG

[0437] TATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAA

[0438] CCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGG

[0439] TCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACT

[0440] GTATTATTCCAGAGTACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAG

[0441] ACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGG

[0442] AACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTG

[0443] GCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGA

[0444] TTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGA

[0445] AACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAACGTGGTAAATTACATTACCAA

[0446] ATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGAATATGTTGCA

[0447] GGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAG

[0448] GAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTA

[0449] GCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCT

[0450] GCTTCCGAACATTCATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGC

[0451] CGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAA

[0452] GTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGAC

[0453] GCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCC

[0454] CACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTTCCTCACACTGTTT

[0455] GTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTGAGAGAACGGCTAACCGG

[0456] ATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGGACAA

[0457] ATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGT

[0458] GGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCG

[0459] GCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGGCTGTTTAAGCTTGGCAAACCTCTG

[0460] GCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACAC

[0461] GCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAA

[0462] ACCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCA

[0463] TTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGAC

[0464] ATAGTCTAGTCCGCCAAGTCTGTTTAAACAGCATATGGGCGCGCCCTCAGCATCGATTC

[0465] AATTCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG

[0466] AACCTTACAACCAGGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCACAAGGGGCGT

[0467] TTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTTCC

[0468] TGCCCTTCTTCAGCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAACGGCACCAAG

[0469] AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCATCGAGAA

[0470] GAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAGC

[0471] CTTCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTG

[0472] CAACGACCCCTTCCTGGACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGG

[0473] GTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGA

[0474] CCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATC

[0475] GACGGCTACTTCAAGATCTACAGCAAGCACACCCCTATCATCGTGAGGGAGCCCGAGGA

[0476] CCTGCCTCAGGGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCCATCGGCATCAACA

[0477] TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACACCTGGAGACAG

[0478] CAGCTCTGGCTGGACAGCTGGAGCCGCTGCTTACTACGTGGGCTACCTGCAACCTAGG

[0479] ACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

[0480] TTGATCCTCTGTCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATC

[0481] TACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

[0482] CACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTAC

[0483] GCTTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACC

[0484] TGGCCCCTTTCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTG

[0485] TGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAAGTGAGGCAGA

[0486] TCGCCCCTGGCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGCCCGACGACTTC ACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGAGCGGCAACT

[0487] ATAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACATC

[0488] AGCACCGAGATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGCCGGCTTCAACT

[0489] GCTACTTCCCCTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTGGACACCAGCCT

[0490] TACAGGGTGGTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCACCGTTTGCGGAC

[0491] CCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACTTCAATGGCCTG

[0492] AAGGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCG

[0493] GCAGGGACATCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACACTGGAGATCCT

[0494] GGACATCACCCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTGGCACCAACACAA

[0495] GCAACCAGGTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGTGCCTGTTGCCAT

[0496] CCACGCCGACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGCAGCAACGTTTTC

[0497] CAGACAAGGGCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACAGCTACGAGTGCG

[0498] ACATCCCCATCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGACCAAGAGCCACAGC

[0499] GTGGCCAGCCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGG

[0500] CCTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAG

[0501] ATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCG

[0502] ACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCTGCACCCAGCTGAA

[0503] GAGGGCCCTGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCAGGAGGTGTTCGCC

[0504] CAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGTACTTCGGCGGCTTCAACTTCAG

[0505] CCAGATCCTGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATCGAGGACCTGCTG

[0506] TTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGAGACTGCTTGG

[0507] GAGACATCGCCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGGCCTGACCGTGTT

[0508] GCCTCCCCTGCTGACCGACGAGATGATCGCTCAGTACACCAGCGCCCTGTTGGCCGGC

[0509] ACCATTACATCTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGATCCCCTTTGCCAT

[0510] GCAGATGGCTTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAAC

[0511] CAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGATAGCCTGA

[0512] GCAGCACCGCTTCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACAACGCCCAGGC

[0513] CCTTAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAGCGTGCTG

[0514] AACGACATCTTCAGCAGGCTGGACccccctGAGGCCGAGGTGCAGATCGACAGGCTGATC

[0515] ACAGGCAGGCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATTAGGGCCGCCG

[0516] AGATCAGGGCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCGTGCTGGGCCA

[0517] GAGCAAGAGAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTTCCCTCAGAGC

[0518] GCCCCTCATGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAGGAGAAGAACTT

[0519] CACCACCGCCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAGGGAGGGAGTG

[0520] TTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCCCAGAT

[0521] CATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTG

[0522] AACAACACCGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGG

[0523] ACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGACATCAGCGGAATT

[0524] AACGCTAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGA

[0525] ACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAA

[0526] GTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCATCGTGATGGTGA

[0527] CCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCTCTTGT

[0528] GGCAGCTGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAG

[0529] CTGCACTACACCtagTCTAGAGTGTTTAAACCGACCCGGGCGGCCGCAACTAACTTAAGC

[0530] TAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTAACGTTACTGGCC

[0531] GAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGC

[0532] CGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCT

[0533] AGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGC

[0534] AGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGC

[0535] GGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATAC

[0536] ACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGA

[0537] GTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACC

[0538] CCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGA

[0539] GGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACA

[0540] CGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTC

[0541] CCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCAC

[0542] ACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCA

[0543] CGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTG

[0544] GCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGC CCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGC

[0545] CTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTC

[0546] TCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAG

[0547] GCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTC

[0548] CCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGAC

[0549] CGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCAAGCTGCTTACA

[0550] TAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTTCTTTTCTTTTC

[0551] CGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAACGCGTCGA

[0552] GGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGC

[0553] GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATA

[0554] ACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT

[0555] GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG

[0556] CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACT

[0557] GGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT

[0558] GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAG

[0559] AGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCA

[0560] CAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC

[0561] ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCT

[0562] AACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG

[0563] AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA

[0564] ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA

[0565] ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGG

[0566] CTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT

[0567] GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGA

[0568] GTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT

[0569] AAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTC

[0570] ATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCT

[0571] TAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCT

[0572] TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA

[0573] GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTC

[0574] AGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTT

[0575] CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTG

[0576] CTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT

[0577] AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGA

[0578] ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC

[0579] CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCG

[0580] CACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC

[0581] ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA

[0582] AACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAACGTTACATAACTTACGGTAAATG

[0583] GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTT

[0584] CCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAA

[0585] ACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTC

[0586] AATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCC

[0587] TACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

[0588] GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCA

[0589] TTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTA

[0590] ACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT

[0591] AAGCAGAGCTTAATACGACTCACTATAGGGCCGGCCATGGGCGGCGCATGAGAGAAGC

[0592] CCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAGACAGCC

[0593] CATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTC

[0594] ACTGATAATGACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAA

[0595] ACGGAGGTGGACCCATCCGACACGATCCTTGACATTGGAAGTGCGCCCGCCCGCAGAA

[0596] TGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACA

[0597] GATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAAT

[0598] TGGACAAGAAAATGAAGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGA

[0599] GACTATGTGCCTCCACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACC

[0600] AGGATGTATACGCGGTTGACGGACCGACAAGTCTCTATCACCAAGCCAATAAGGGAGTT

[0601] AGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTAAGAACTTGGCTGGA

[0602] GCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATA GGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAA

[0603] GAAGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGA

[0604] GAAGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGTATTTCACTTACGTGGCAAGC AA

[0605] SEQ ID NO: 3: CMV+T7_Codon optimized omicron spike with Kozak and Shine- Dalgano (Dual RBS system)

[0606] AATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATA

[0607] GCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGA

[0608] GGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCG

[0609] TGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGAT

[0610] GTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTATAGTCGTCA

[0611] ACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCGTAGTGGCC

[0612] CAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACT

[0613] AGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGA

[0614] TAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCAAAGTGAACAGCGATTTCC

[0615] ACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAGATCGGGCTGAGAACAAGA

[0616] ATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGT

[0617] ACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTG

[0618] CGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAGGCAGACG

[0619] TCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCTTGATA

[0620] AAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTGCTTTCTCCGCA

[0621] GGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGT

[0622] GATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAG

[0623] TGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCC

[0624] ACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACCTGCACCATATTGCCACACAT

[0625] GGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGA

[0626] CGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTG

[0627] GGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAG

[0628] TCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTGC

[0629] CAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCACCAAAAAAGATCTAGTGGTG

[0630] AGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAGGGCT

[0631] GGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAG

[0632] AGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAG

[0633] CCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGATCCCAAACAGTGCGGTTTTTTTA

[0634] ACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCACACAAGTCTTCCACAAAA

[0635] GCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACA

[0636] AAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGT

[0637] ACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTT

[0638] GCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACCC

[0639] GTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCACCCACC

[0640] TCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACT

[0641] AGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCA

[0642] CGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACC

[0643] GGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGTTGGGCCAAGGCTTTAGTGC

[0644] CGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTGTGGATTAT

[0645] TTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTC

[0646] TTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGG

[0647] AATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGT

[0648] CCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGAGTCT

[0649] ATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTACCTGTAA

[0650] ACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTT

[0651] CTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCC

[0652] GTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCG

[0653] GCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACATAATATTTGTTAATGTGAG

[0654] GACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTAGCAT GTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTT ATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAG

[0655] TTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATTC

[0656] ATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAACCTTGACC

[0657] AACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGGTG

[0658] CGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGG

[0659] ACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGAT

[0660] TTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAAAGGTGCAGCTAAACATATCAT

[0661] TCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTGACAAACAGTTGG

[0662] CAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGA

[0663] TTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGA

[0664] ACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACA

[0665] AGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGAT

[0666] ATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGGGTGCATC

[0667] CGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCA

[0668] TATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCAT

[0669] GTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCATGTATATCCTCGGAGAAAGC

[0670] ATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGGAAGCCTCCACACCACCTAG

[0671] CACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAAAAG

[0672] CCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCA

[0673] CTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCG

[0674] TATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCC

[0675] ATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGAGG

[0676] ATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAG

[0677] CATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTC

[0678] ACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATG

[0679] TGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTAGCGTGACCAGCGGGGCAAC

[0680] GTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGCGACCGGTGC

[0681] CTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCG

[0682] TCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCG

[0683] TGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAG

[0684] CAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATT

[0685] ACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTGC

[0686] ATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAAC

[0687] GGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGC

[0688] CTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACAGTTAAATCCCACACCTGCT

[0689] AACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCATAACAGCTAGACG

[0690] TATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAA

[0691] CCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGG

[0692] TCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACT

[0693] GTATTATTCCAGAGTACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAG

[0694] ACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGG

[0695] AACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTG

[0696] GCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGA

[0697] TTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGA

[0698] AACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAACGTGGTAAATTACATTACCAA

[0699] ATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGAATATGTTGCA

[0700] GGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAG

[0701] GAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTA

[0702] GCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCT

[0703] GCTTCCGAACATTCATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGC

[0704] CGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAA

[0705] GTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGAC

[0706] GCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCC

[0707] CACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTTCCTCACACTGTTT

[0708] GTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTGAGAGAACGGCTAACCGG

[0709] ATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGGACAA

[0710] ATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGT

[0711] GGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCG

[0712] GCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGGCTGTTTAAGCTTGGCAAACCTCTG GCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACAC

[0713] GCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAA

[0714] ACCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCA

[0715] TTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGAC

[0716] ATAGTCTAGTCCGCCAAGTCTGTTTAAACAGCATATGGGCGCGCCCTCAGCATCGATTC

[0717] AATTtAAGGAGGTcGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTG AGO AGCO

[0718] AGTGCGTGAACCTTACAACCAGGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCACA

[0719] AGGGGCGTTTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGG

[0720] ACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAAC

[0721] GGCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCA

[0722] GCATCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAA

[0723] GACCCAGAGCCTTCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGT

[0724] TCCAGTTCTGCAACGACCCCTTCCTGGACCACAAGAACAACAAGAGCTGGATGGAGAGC

[0725] GAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTT

[0726] CCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTC

[0727] AAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCTATCATCGTGAGGGA

[0728] GCCCGAGGACCTGCCTCAGGGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCCATC

[0729] GGCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACAC

[0730] CTGGAGACAGCAGCTCTGGCTGGACAGCTGGAGCCGCTGCTT ACT ACGTGGGCT ACCT

[0731] GCAACCTAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTG

[0732] GACTGCGCCCTTGATCCTCTGTCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGA

[0733] GAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGG

[0734] TTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCGC

[0735] CAGCGTGTACGCTTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTG

[0736] CTGTACAACCTGGCCCCTTTCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCT

[0737] GAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAA

[0738] GTGAGGCAGATCGCCCCTGGCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGC

[0739] CCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGT

[0740] GAGCGGCAACTATAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

[0741] AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGC

[0742] CGGCTTCAACTGCTACTTCCCCTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTG

[0743] GACACCAGCCTTACAGGGTGGTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCAC

[0744] CGTTTGCGGACCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACT

[0745] TCAATGGCCTGAAGGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTT

[0746] CCAGCAGTTCGGCAGGGACATCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACA

[0747] CTGGAGATCCTGGACATCACCCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTG

[0748] GCACCAACACAAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGT

[0749] GCCTGTTGCCATCCACGCCGACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGC

[0750] AGCAACGTTTTCCAGACAAGGGCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACA

[0751] GCTACGAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGAC

[0752] CAAGAGCCACAGCGTGGCCAGCCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCC

[0753] GAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAG

[0754] CGTGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATG

[0755] TACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCT

[0756] GCACCCAGCTGAAGAGGGCCCTGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCA

[0757] GGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGTACTTCGGCG

[0758] GCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATC

[0759] GAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

[0760] GAGACTGCTTGGGAGACATCGCCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGG

[0761] CCTGACCGTGTTGCCTCCCCTGCTGACCGACGAGATGATCGCTCAGTACACCAGCGCC

[0762] CTGTTGGCCGGCACCATTACATCTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGAT

[0763] CCCCTTTGCCATGCAGATGGCTTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGC

[0764] TGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAG

[0765] GATAGCCTGAGCAGCACCGCTTCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACA

[0766] ACGCCCAGGCCCTTAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAG

[0767] CAGCGTGCTGAACGACATCTTCAGCAGGCTGGACccccctGAGGCCGAGGTGCAGATCGA

[0768] CAGGCTGATCACAGGCAGGCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATT

[0769] AGGGCCGCCGAGATCAGGGCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCG

[0770] TGCTGGGCCAGAGCAAGAGAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTT CCCTCAGAGCGCCCCTCATGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAG

[0771] GAGAAGAACTTCACCACCGCCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAG

[0772] GGAGGGAGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTAC

[0773] GAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGA

[0774] TCGGCATCGTGAACAACACCGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAA

[0775] GGAGGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGAC

[0776] ATCAGCGGAATTAACGCTAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGA

[0777] GGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

[0778] CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCAT

[0779] CGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGC

[0780] TGCTGCTCTTGTGGCAGCTGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGA

[0781] AGGGCGTGAAGCTGCACTACACCtagTCTAGAGTGTTTAAACCGACCCGGGCGGCCGCA

[0782] ACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTA

[0783] ACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTT

[0784] CCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTG

[0785] ACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGT

[0786] CGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCC

[0787] TTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACG

[0788] TGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAG

[0789] TTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCC

[0790] CAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATG

[0791] TGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCC

[0792] TTTGAAAAACACGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCG

[0793] CGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGC

[0794] CACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAA

[0795] CTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGC

[0796] GCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGC

[0797] CGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACA

[0798] GATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCAC

[0799] CGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCC

[0800] CGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGC

[0801] CCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGT

[0802] GCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCA

[0803] AGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTT

[0804] TCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAA

[0805] AACGCGTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGG

[0806] AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC

[0807] ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTC

[0808] AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCA

[0809] CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTT

[0810] ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT

[0811] TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGAC

[0812] GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTA

[0813] CTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG

[0814] CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA

[0815] CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG

[0816] TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT

[0817] GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC

[0818] CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTC

[0819] GGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTC

[0820] GCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC

[0821] ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG

[0822] CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA

[0823] TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGA

[0824] CCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCA

[0825] AAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC

[0826] ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGG

[0827] TAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG

[0828] GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG

[0829] TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT

[0830] TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGC

[0831] CACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAAC

[0832] AGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTC

[0833] GGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGA

[0834] GCCTATGGAAAAACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAACGTTACATAACTT

[0835] ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAA

[0836] TGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGT

[0837] ATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCC

[0838] CTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTAT

[0839] GGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGC

[0840] GGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGT

[0841] CTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCA

[0842] AAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGG

[0843] AGGTCTATATAAGCAGAGCTTAATACGACTCACTATAGGGCCGGCCATGGGCGGCGCAT

[0844] GAGAGAAGCCCAGACCAATTACCTtAAGGAGGTcCCCAAAATGGAGAAAGTTCACGTTGA

[0845] CATCGAGGAAGACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGG

[0846] TAGAAGCCAAGCAGGTCACTGATAATGACCATGCTAATGCCAGAGCGTTTTCGCATCTG

[0847] GCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGATCCTTGACATTGGAAG

[0848] TGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGATG

[0849] TGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGG

[0850] AAATAACTGATAAGGAATTGGACAAGAAAATGAAGGAGCTGGCCGCCGTCATGAGCGAC

[0851] CCTGACCTGGAAACTGAGACTATGTGCCTCCACGACGACGAGTCGTGTCGCTACGAAG

[0852] GGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAAGTCTCTATCAC

[0853] CAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTAT

[0854] GTTTAAGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCG

[0855] TGTTAACGGCTCGTAACATAGGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGA

[0856] GGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTG

[0857] GCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGT

[0858] ATTTCACTTACGTGGCAAGCAA

[0859] SEQ ID NO: 4: T7_Codon optimized Omicron spike with Kozak RBS and Shine- dalgano Ribosomal binding site (RBS)

[0860] AATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATA

[0861] GCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGA

[0862] GGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCG

[0863] TGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGAT

[0864] GTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTATAGTCGTCA

[0865] ACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCGTAGTGGCC

[0866] CAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACT

[0867] AGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGA

[0868] TAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCAAAGTGAACAGCGATTTCC

[0869] ACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAGATCGGGCTGAGAACAAGA

[0870] ATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGT

[0871] ACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTG

[0872] CGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAGGCAGACG

[0873] TCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCTTGATA

[0874] AAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTGCTTTCTCCGCA

[0875] GGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGT

[0876] GATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAG

[0877] TGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCC

[0878] ACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACCTGCACCATATTGCCACACAT

[0879] GGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGA

[0880] CGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTG

[0881] GGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAG

[0882] TCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTGC

[0883] CAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCACCAAAAAAGATCTAGTGGTG AGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAGGGCT

[0884] GGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAG

[0885] AGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAG

[0886] CCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGATCCCAAACAGTGCGGTTTTTTTA

[0887] ACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCACACAAGTCTTCCACAAAA

[0888] GCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACA

[0889] AAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGT

[0890] ACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTT

[0891] GCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACCC

[0892] GTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCACCCACC

[0893] TCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACT

[0894] AGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCA

[0895] CGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACC

[0896] GGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGTTGGGCCAAGGCTTTAGTGC

[0897] CGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTGTGGATTAT

[0898] TTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTC

[0899] TTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGG

[0900] AATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGT

[0901] CCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGAGTCT

[0902] ATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTACCTGTAA

[0903] ACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTT

[0904] CTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCC

[0905] GTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCG

[0906] GCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACATAATATTTGTTAATGTGAG

[0907] GACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTAGCAT

[0908] GTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTT

[0909] ATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAG

[0910] TTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATTC

[0911] ATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAACCTTGACC

[0912] AACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGGTG

[0913] CGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGG

[0914] ACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGAT

[0915] TTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAAAGGTGCAGCTAAACATATCAT

[0916] TCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTGACAAACAGTTGG

[0917] CAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGA

[0918] TTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGA

[0919] ACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACA

[0920] AGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGAT

[0921] ATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGGGTGCATC

[0922] CGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCA

[0923] TATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCAT

[0924] GTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCATGTATATCCTCGGAGAAAGC

[0925] ATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGGAAGCCTCCACACCACCTAG

[0926] CACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAAAAG

[0927] CCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCA

[0928] CTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCG

[0929] TATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCC

[0930] ATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGAGG

[0931] ATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAG

[0932] CATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTC

[0933] ACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATG

[0934] TGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTAGCGTGACCAGCGGGGCAAC

[0935] GTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGCGACCGGTGC

[0936] CTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCG

[0937] TCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCG

[0938] TGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAG

[0939] CAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATT

[0940] ACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTGC

[0941] ATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAAC GGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGC

[0942] CTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACAGTTAAATCCCACACCTGCT

[0943] AACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCATAACAGCTAGACG

[0944] TATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAA

[0945] CCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGG

[0946] TCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACT

[0947] GTATTATTCCAGAGTACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAG

[0948] ACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGG

[0949] AACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTG

[0950] GCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGA

[0951] TTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGA

[0952] AACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAACGTGGTAAATTACATTACCAA

[0953] ATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGAATATGTTGCA

[0954] GGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAG

[0955] GAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTA

[0956] GCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCT

[0957] GCTTCCGAACATTCATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGC

[0958] CGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAA

[0959] GTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGAC

[0960] GCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCC

[0961] CACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTTCCTCACACTGTTT

[0962] GTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTGAGAGAACGGCTAACCGG

[0963] ATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGGACAA

[0964] ATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGT

[0965] GGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCG

[0966] GCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGGCTGTTTAAGCTTGGCAAACCTCTG

[0967] GCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACAC

[0968] GCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAA

[0969] ACCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCA

[0970] TTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGAC

[0971] ATAGTCTAGTCCGCCAAGTCTGTTTAAACAGCATATGGGCGCGCCCTCAGCATCGATTC

[0972] AATTtAAGGAGGTcgccaccATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCA

[0973] GTGCGTGAACCTTACAACCAGGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCACAA

[0974] GGGGCGTTTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

[0975] CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAACG

[0976] GCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAG

[0977] CATCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAG

[0978] ACCCAGAGCCTTCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTT

[0979] CCAGTTCTGCAACGACCCCTTCCTGGACCACAAGAACAACAAGAGCTGGATGGAGAGC

[0980] GAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTT

[0981] CCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTC

[0982] AAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCTATCATCGTGAGGGA

[0983] GCCCGAGGACCTGCCTCAGGGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCCATC

[0984] GGCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACAC

[0985] CTGGAGACAGCAGCTCTGGCTGGACAGCTGGAGCCGCTGCTT ACT ACGTGGGCT ACCT

[0986] GCAACCTAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTG

[0987] GACTGCGCCCTTGATCCTCTGTCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGA

[0988] GAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGG

[0989] TTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCGC

[0990] CAGCGTGTACGCTTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTG

[0991] CTGTACAACCTGGCCCCTTTCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCT

[0992] GAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAA

[0993] GTGAGGCAGATCGCCCCTGGCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGC

[0994] CCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGT

[0995] GAGCGGCAACTATAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

[0996] AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGC

[0997] CGGCTTCAACTGCTACTTCCCCTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTG

[0998] GACACCAGCCTTACAGGGTGGTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCAC

[0999] CGTTTGCGGACCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACT TCAATGGCCTGAAGGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTT

[1000] CCAGCAGTTCGGCAGGGACATCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACA

[1001] CTGGAGATCCTGGACATCACCCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTG

[1002] GCACCAACACAAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGT

[1003] GCCTGTTGCCATCCACGCCGACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGC

[1004] AGCAACGTTTTCCAGACAAGGGCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACA

[1005] GCTACGAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGAC

[1006] CAAGAGCCACAGCGTGGCCAGCCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCC

[1007] GAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAG

[1008] CGTGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATG

[1009] TACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCT

[1010] GCACCCAGCTGAAGAGGGCCCTGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCA

[1011] GGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGTACTTCGGCG

[1012] GCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATC

[1013] GAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

[1014] GAGACTGCTTGGGAGACATCGCCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGG

[1015] CCTGACCGTGTTGCCTCCCCTGCTGACCGACGAGATGATCGCTCAGTACACCAGCGCC

[1016] CTGTTGGCCGGCACCATTACATCTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGAT

[1017] CCCCTTTGCCATGCAGATGGCTTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGC

[1018] TGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAG

[1019] GATAGCCTGAGCAGCACCGCTTCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACA

[1020] ACGCCCAGGCCCTTAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAG

[1021] CAGCGTGCTGAACGACATCTTCAGCAGGCTGGACccccctGAGGCCGAGGTGCAGATCGA

[1022] CAGGCTGATCACAGGCAGGCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATT

[1023] AGGGCCGCCGAGATCAGGGCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCG

[1024] TGCTGGGCCAGAGCAAGAGAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTT

[1025] CCCTCAGAGCGCCCCTCATGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAG

[1026] GAGAAGAACTTCACCACCGCCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAG

[1027] GGAGGGAGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTAC

[1028] GAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGA

[1029] TCGGCATCGTGAACAACACCGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAA

[1030] GGAGGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGAC

[1031] ATCAGCGGAATTAACGCTAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGA

[1032] GGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

[1033] CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCAT

[1034] CGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGC

[1035] TGCTGCTCTTGTGGCAGCTGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGA

[1036] AGGGCGTGAAGCTGCACTACACCtagTCTAGAGTGTTTAAACCGACCCGGGCGGCCGCA

[1037] ACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTA

[1038] ACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTT

[1039] CCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTG

[1040] ACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGT

[1041] CGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCC

[1042] TTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACG

[1043] TGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAG

[1044] TTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCC

[1045] CAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATG

[1046] TGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCC

[1047] TTTGAAAAACACGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCG

[1048] CGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGC

[1049] CACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAA

[1050] CTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGC

[1051] GCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGC

[1052] CGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACA

[1053] GATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCAC

[1054] CGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCC

[1055] CGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGC

[1056] CCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGT

[1057] GCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCA AGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTT

[1058] TCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAA

[1059] AACGCGTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGG

[1060] AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC

[1061] ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTC

[1062] AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCA

[1063] CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTT

[1064] ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT

[1065] TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGAC

[1066] GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTA

[1067] CTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG

[1068] CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA

[1069] CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG

[1070] TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT

[1071] GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC

[1072] CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTC

[1073] GGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTC

[1074] GCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC

[1075] ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG

[1076] CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA

[1077] TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGA

[1078] CCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCA

[1079] AAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC

[1080] ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGG

[1081] TAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG

[1082] GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC

[1083] CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG

[1084] TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT

[1085] TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGC

[1086] CACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAAC

[1087] AGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTC

[1088] GGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGA

[1089] GCCTATGGAAAAACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAATAATACGACTCA

[1090] CTATAGGGCCGGCCATGGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTtAAGGAG

[1091] GTcCCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAGACAGCCCATTCCTCAGAGC

[1092] TTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATGACC

[1093] ATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGAC

[1094] CCATCCGACACGATCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCA

[1095] CAAGTATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACAGATTGTATAAGTA

[1096] TGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAATTGGACAAGAAAAT

[1097] GAAGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTC

[1098] CACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGC

[1099] GGTTGACGGACCGACAAGTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACT

[1100] GGATAGGCTTTGACACCACCCCTTTTATGTTTAAGAACTTGGCTGGAGCATATCCATCAT

[1101] ACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAGGCCTATGCAGC

[1102] TCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAA

[1103] CCATCCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTA

[1104] CTGAGGAGCTGGCACCTGCCGTCTGTATTTCACTTACGTGGCAAGCAA

[1105] SEQ ID NO: 5: Lac Promoter_codon optimized omicron spike with Kozak and SD RBS

[1106] AATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATA

[1107] GCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGA GGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCG

[1108] TGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGAT GTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTATAGTCGTCA

[1109] ACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCGTAGTGGCC CAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACT

[1110] AGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGA

[1111] TAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCAAAGTGAACAGCGATTTCC

[1112] ACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAGATCGGGCTGAGAACAAGA

[1113] ATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGT

[1114] ACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTG

[1115] CGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAGGCAGACG

[1116] TCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCTTGATA

[1117] AAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTGCTTTCTCCGCA

[1118] GGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGT

[1119] GATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAG

[1120] TGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCC

[1121] ACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACCTGCACCATATTGCCACACAT

[1122] GGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGA

[1123] CGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTG

[1124] GGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAG

[1125] TCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTGC

[1126] CAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCACCAAAAAAGATCTAGTGGTG

[1127] AGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAGGGCT

[1128] GGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAG

[1129] AGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAG

[1130] CCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGATCCCAAACAGTGCGGTTTTTTTA

[1131] ACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCACACAAGTCTTCCACAAAA

[1132] GCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACA

[1133] AAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGT

[1134] ACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTT

[1135] GCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACCC

[1136] GTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCACCCACC

[1137] TCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACT

[1138] AGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCA

[1139] CGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACC

[1140] GGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGTTGGGCCAAGGCTTTAGTGC

[1141] CGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTGTGGATTAT

[1142] TTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTC

[1143] TTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGG

[1144] AATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGT

[1145] CCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGAGTCT

[1146] ATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTACCTGTAA

[1147] ACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTT

[1148] CTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCC

[1149] GTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCG

[1150] GCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACATAATATTTGTTAATGTGAG

[1151] GACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTAGCAT

[1152] GTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTT

[1153] ATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAG

[1154] TTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATTC

[1155] ATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAACCTTGACC

[1156] AACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGGTG

[1157] CGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGG

[1158] ACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGAT

[1159] TTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAAAGGTGCAGCTAAACATATCAT

[1160] TCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTGACAAACAGTTGG

[1161] CAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGA

[1162] TTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGA

[1163] ACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACA

[1164] AGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGAT

[1165] ATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGGGTGCATC

[1166] CGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCA

[1167] TATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCAT GTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCATGTATATCCTCGGAGAAAGC

[1168] ATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGGAAGCCTCCACACCACCTAG

[1169] CACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAAAAG

[1170] CCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCA

[1171] CTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCG

[1172] TATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCC

[1173] ATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGAGG

[1174] ATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAG

[1175] CATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTC

[1176] ACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATG

[1177] TGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTAGCGTGACCAGCGGGGCAAC

[1178] GTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGCGACCGGTGC

[1179] CTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCG

[1180] TCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCG

[1181] TGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAG

[1182] CAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATT

[1183] ACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTGC

[1184] ATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAAC

[1185] GGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGC

[1186] CTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACAGTTAAATCCCACACCTGCT

[1187] AACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCATAACAGCTAGACG

[1188] TATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAA

[1189] CCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGG

[1190] TCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACT

[1191] GTATTATTCCAGAGTACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAG

[1192] ACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGG

[1193] AACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTG

[1194] GCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGA

[1195] TTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGA

[1196] AACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAACGTGGTAAATTACATTACCAA

[1197] ATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGAATATGTTGCA

[1198] GGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAG

[1199] GAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTA

[1200] GCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCT

[1201] GCTTCCGAACATTCATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGC

[1202] CGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAA

[1203] GTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGAC

[1204] GCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCC

[1205] CACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTTCCTCACACTGTTT

[1206] GTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTGAGAGAACGGCTAACCGG

[1207] ATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGGACAA

[1208] ATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGT

[1209] GGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCG

[1210] GCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGGCTGTTTAAGCTTGGCAAACCTCTG

[1211] GCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACAC

[1212] GCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAA

[1213] ACCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCA

[1214] TTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGAC

[1215] ATAGTCTAGTCCGCCAAGTCTGTTTAAACAGCATATGGGCGCGCCCTCAGCATCGATTC

[1216] AATTtAAGGAGGTcgccaccATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCA

[1217] GTGCGTGAACCTTACAACCAGGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCACAA

[1218] GGGGCGTTTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

[1219] CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAACG

[1220] GCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAG

[1221] CATCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAG

[1222] ACCCAGAGCCTTCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTT

[1223] CCAGTTCTGCAACGACCCCTTCCTGGACCACAAGAACAACAAGAGCTGGATGGAGAGC

[1224] GAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTT

[1225] CCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTC AAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCTATCATCGTGAGGGA

[1226] GCCCGAGGACCTGCCTCAGGGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCCATC

[1227] GGCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACAC

[1228] CTGGAGACAGCAGCTCTGGCTGGACAGCTGGAGCCGCTGCTT ACT ACGTGGGCT ACCT

[1229] GCAACCTAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTG

[1230] GACTGCGCCCTTGATCCTCTGTCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGA

[1231] GAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGG

[1232] TTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCGC

[1233] CAGCGTGTACGCTTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTG

[1234] CTGTACAACCTGGCCCCTTTCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCT

[1235] GAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAA

[1236] GTGAGGCAGATCGCCCCTGGCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGC

[1237] CCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGT

[1238] GAGCGGCAACTATAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

[1239] AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGC

[1240] CGGCTTCAACTGCTACTTCCCCTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTG

[1241] GACACCAGCCTTACAGGGTGGTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCAC

[1242] CGTTTGCGGACCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACT

[1243] TCAATGGCCTGAAGGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTT

[1244] CCAGCAGTTCGGCAGGGACATCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACA

[1245] CTGGAGATCCTGGACATCACCCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTG

[1246] GCACCAACACAAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGT

[1247] GCCTGTTGCCATCCACGCCGACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGC

[1248] AGCAACGTTTTCCAGACAAGGGCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACA

[1249] GCTACGAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGAC

[1250] CAAGAGCCACAGCGTGGCCAGCCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCC

[1251] GAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAG

[1252] CGTGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATG

[1253] TACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCT

[1254] GCACCCAGCTGAAGAGGGCCCTGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCA

[1255] GGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGTACTTCGGCG

[1256] GCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATC

[1257] GAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

[1258] GAGACTGCTTGGGAGACATCGCCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGG

[1259] CCTGACCGTGTTGCCTCCCCTGCTGACCGACGAGATGATCGCTCAGTACACCAGCGCC

[1260] CTGTTGGCCGGCACCATTACATCTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGAT

[1261] CCCCTTTGCCATGCAGATGGCTTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGC

[1262] TGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAG

[1263] GATAGCCTGAGCAGCACCGCTTCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACA

[1264] ACGCCCAGGCCCTTAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAG

[1265] CAGCGTGCTGAACGACATCTTCAGCAGGCTGGACccccctGAGGCCGAGGTGCAGATCGA

[1266] CAGGCTGATCACAGGCAGGCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATT

[1267] AGGGCCGCCGAGATCAGGGCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCG

[1268] TGCTGGGCCAGAGCAAGAGAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTT

[1269] CCCTCAGAGCGCCCCTCATGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAG

[1270] GAGAAGAACTTCACCACCGCCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAG

[1271] GGAGGGAGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTAC

[1272] GAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGA

[1273] TCGGCATCGTGAACAACACCGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAA

[1274] GGAGGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGAC

[1275] ATCAGCGGAATTAACGCTAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGA

[1276] GGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

[1277] CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCAT

[1278] CGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGC

[1279] TGCTGCTCTTGTGGCAGCTGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGA

[1280] AGGGCGTGAAGCTGCACTACACCtagTCTAGAGTGTTTAAACCGACCCGGGCGGCCGCA

[1281] ACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTA

[1282] ACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTT

[1283] CCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTG ACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGT

[1284] CGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCC

[1285] TTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACG

[1286] TGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAG

[1287] TTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCC

[1288] CAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATG

[1289] TGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCC

[1290] TTTGAAAAACACGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCG

[1291] CGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGC

[1292] CACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAA

[1293] CTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGC

[1294] GCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGC

[1295] CGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACA

[1296] GATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCAC

[1297] CGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCC

[1298] CGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGC

[1299] CCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGT

[1300] GCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCA

[1301] AGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTT

[1302] TCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAA

[1303] AACGCGTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGG

[1304] AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC

[1305] ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTC

[1306] AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCA

[1307] CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTT

[1308] ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT

[1309] TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGAC

[1310] GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTA

[1311] CTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG

[1312] CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA

[1313] CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG

[1314] TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT

[1315] GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC

[1316] CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTC

[1317] GGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTC

[1318] GCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC

[1319] ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG

[1320] CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA

[1321] TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGA

[1322] CCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCA

[1323] AAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC

[1324] ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGG

[1325] TAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG

[1326] GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC

[1327] CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG

[1328] TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT

[1329] TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGC

[1330] CACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAAC

[1331] AGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTC

[1332] GGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGA

[1333] GCCTATGGAAAAACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAATTTACACTTTATG

[1334] CTTCCGGCTCGTATGTTGGCCGGCCATGGGCGGCGCATGAGAGAAGCCCAGACCAATT

[1335] ACCTtAAGGAGGTcCCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAGACAGCCCAT

[1336] TCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACT

[1337] GATAATGACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACG

[1338] GAGGTGGACCCATCCGACACGATCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGT

[1339] ATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACAGAT

[1340] TGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAATTGG

[1341] ACAAGAAAATGAAGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGAC TATGTGCCTCCACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGG

[1342] ATGTATACGCGGTTGACGGACCGACAAGTCTCTATCACCAAGCCAATAAGGGAGTTAGA

[1343] GTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTAAGAACTTGGCTGGAGCA

[1344] TATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAGG

[1345] CCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGA

[1346] AGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGA

[1347] AGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGTATTTCACTTACGTGGCAAGCAA

[1348] SEQ ID NO: 6: Lac promoter with prokaryotic RBS only

[1349] AATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATA

[1350] GCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGA

[1351] GGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCG

[1352] TGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGAT

[1353] GTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTATAGTCGTCA

[1354] ACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCGTAGTGGCC

[1355] CAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACT

[1356] AGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGA

[1357] TAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCAAAGTGAACAGCGATTTCC

[1358] ACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAGATCGGGCTGAGAACAAGA

[1359] ATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGT

[1360] ACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTG

[1361] CGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAGGCAGACG

[1362] TCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCTTGATA

[1363] AAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTGCTTTCTCCGCA

[1364] GGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGT

[1365] GATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAG

[1366] TGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCC

[1367] ACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACCTGCACCATATTGCCACACAT

[1368] GGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGA

[1369] CGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTG

[1370] GGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAG

[1371] TCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTGC

[1372] CAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCACCAAAAAAGATCTAGTGGTG

[1373] AGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAGGGCT

[1374] GGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAG

[1375] AGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAG

[1376] CCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGATCCCAAACAGTGCGGTTTTTTTA

[1377] ACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCACACAAGTCTTCCACAAAA

[1378] GCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACA

[1379] AAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGT

[1380] ACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTT

[1381] GCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACCC

[1382] GTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCACCCACC

[1383] TCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACT

[1384] AGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCA

[1385] CGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACC

[1386] GGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGTTGGGCCAAGGCTTTAGTGC

[1387] CGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTGTGGATTAT

[1388] TTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTC

[1389] TTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGG

[1390] AATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGT

[1391] CCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGAGTCT

[1392] ATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTACCTGTAA

[1393] ACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTT

[1394] CTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCC

[1395] GTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCG

[1396] GCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACATAATATTTGTTAATGTGAG

[1397] GACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTAGCAT GTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTT

[1398] ATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAG

[1399] TTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATTC

[1400] ATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAACCTTGACC

[1401] AACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGGTG

[1402] CGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGG

[1403] ACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGAT

[1404] TTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAAAGGTGCAGCTAAACATATCAT

[1405] TCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTGACAAACAGTTGG

[1406] CAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGA

[1407] TTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGA

[1408] ACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACA

[1409] AGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGAT

[1410] ATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGGGTGCATC

[1411] CGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCA

[1412] TATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCAT

[1413] GTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCATGTATATCCTCGGAGAAAGC

[1414] ATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGGAAGCCTCCACACCACCTAG

[1415] CACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAAAAG

[1416] CCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCA

[1417] CTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCG

[1418] TATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCC

[1419] ATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGAGG

[1420] ATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAG

[1421] CATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTC

[1422] ACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATG

[1423] TGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTAGCGTGACCAGCGGGGCAAC

[1424] GTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGCGACCGGTGC

[1425] CTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCG

[1426] TCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCG

[1427] TGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAG

[1428] CAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATT

[1429] ACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTGC

[1430] ATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAAC

[1431] GGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGC

[1432] CTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACAGTTAAATCCCACACCTGCT

[1433] AACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCATAACAGCTAGACG

[1434] TATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAA

[1435] CCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGG

[1436] TCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACT

[1437] GTATTATTCCAGAGTACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAG

[1438] ACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGG

[1439] AACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTG

[1440] GCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGA

[1441] TTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGA

[1442] AACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAACGTGGTAAATTACATTACCAA

[1443] ATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGAATATGTTGCA

[1444] GGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAG

[1445] GAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTA

[1446] GCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCT

[1447] GCTTCCGAACATTCATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGC

[1448] CGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAA

[1449] GTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGAC

[1450] GCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCC

[1451] CACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTTCCTCACACTGTTT

[1452] GTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTGAGAGAACGGCTAACCGG

[1453] ATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGGACAA

[1454] ATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGT

[1455] GGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCG GCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGGCTGTTTAAGCTTGGCAAACCTCTG

[1456] GCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACAC

[1457] GCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAA

[1458] ACCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCA

[1459] TTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGAC

[1460] ATAGTCTAGTCCGCCAAGTCTGTTTAAACAGCATATGGGCGCGCCCTCAGCATCGATTC

[1461] AATTtAAGGAGGTcATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG

[1462] TGAACCTTACAACCAGGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCACAAGGGGC

[1463] GTTTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTT

[1464] CCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAACGGCACCA

[1465] AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCATCGA

[1466] GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAG

[1467] AGCCTTCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTT

[1468] CTGCAACGACCCCTTCCTGGACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

[1469] GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGAT

[1470] GGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCAAGAAC

[1471] ATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCTATCATCGTGAGGGAGCCCGA

[1472] GGACCTGCCTCAGGGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCCATCGGCATCA

[1473] ACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACACCTGGAGA

[1474] CAGCAGCTCTGGCTGGACAGCTGGAGCCGCTGCTTACTACGTGGGCTACCTGCAACCT

[1475] AGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCG

[1476] CCCTTGATCCTCTGTCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGC

[1477] ATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAA

[1478] CATCACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

[1479] ACGCTTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAA

[1480] CCTGGCCCCTTTCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACC

[1481] TGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAAGTGAGGCA

[1482] GATCGCCCCTGGCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGCCCGACGACT

[1483] TCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGAGCGGCAA

[1484] CTATAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACA

[1485] TCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGCCGGCTTCAA

[1486] CTGCTACTTCCCCTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTGGACACCAGC

[1487] CTTACAGGGTGGTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCACCGTTTGCGGA

[1488] CCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACTTCAATGGCCT

[1489] GAAGGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTC

[1490] GGCAGGGACATCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACACTGGAGATCC

[1491] TGGACATCACCCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTGGCACCAACACA

[1492] AGCAACCAGGTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGTGCCTGTTGCCA

[1493] TCCACGCCGACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGCAGCAACGTTTTC

[1494] CAGACAAGGGCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACAGCTACGAGTGCG

[1495] ACATCCCCATCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGACCAAGAGCCACAGC

[1496] GTGGCCAGCCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGG

[1497] CCTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAG

[1498] ATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCG

[1499] ACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCTGCACCCAGCTGAA

[1500] GAGGGCCCTGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCAGGAGGTGTTCGCC

[1501] CAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGTACTTCGGCGGCTTCAACTTCAG

[1502] CCAGATCCTGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATCGAGGACCTGCTG

[1503] TTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGAGACTGCTTGG

[1504] GAGACATCGCCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGGCCTGACCGTGTT

[1505] GCCTCCCCTGCTGACCGACGAGATGATCGCTCAGTACACCAGCGCCCTGTTGGCCGGC

[1506] ACCATTACATCTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGATCCCCTTTGCCAT

[1507] GCAGATGGCTTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAAC

[1508] CAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGATAGCCTGA

[1509] GCAGCACCGCTTCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACAACGCCCAGGC

[1510] CCTTAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAGCGTGCTG

[1511] AACGACATCTTCAGCAGGCTGGACccccctGAGGCCGAGGTGCAGATCGACAGGCTGATC

[1512] ACAGGCAGGCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATTAGGGCCGCCG

[1513] AGATCAGGGCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCGTGCTGGGCCA GAGCAAGAGAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTTCCCTCAGAGC

[1514] GCCCCTCATGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAGGAGAAGAACTT

[1515] CACCACCGCCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAGGGAGGGAGTG

[1516] TTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCCCAGAT

[1517] CATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTG

[1518] AACAACACCGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGG

[1519] ACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGACATCAGCGGAATT

[1520] AACGCTAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGA

[1521] ACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAA

[1522] GTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCATCGTGATGGTGA

[1523] CCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCTCTTGT

[1524] GGCAGCTGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAG

[1525] CTGCACTACACCtagTCTAGAGTGTTTAAACCGACCCGGGCGGCCGCAACTAACTTAAGC

[1526] TAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTAACGTTACTGGCC

[1527] GAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGC

[1528] CGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCT

[1529] AGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGC

[1530] AGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGC

[1531] GGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATAC

[1532] ACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGA

[1533] GTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACC

[1534] CCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGA

[1535] GGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACA

[1536] CGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTC

[1537] CCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCAC

[1538] ACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCA

[1539] CGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTG

[1540] GCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGC

[1541] CCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGC

[1542] CTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTC

[1543] TCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAG

[1544] GCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTC

[1545] CCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGAC

[1546] CGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCAAGCTGCTTACA

[1547] TAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTTCTTTTCTTTTC

[1548] CGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAACGCGTCGA

[1549] GGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGC

[1550] GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATA

[1551] ACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT

[1552] GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG

[1553] CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACT

[1554] GGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT

[1555] GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAG

[1556] AGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCA

[1557] CAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC

[1558] ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCT

[1559] AACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG

[1560] AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA

[1561] ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA

[1562] ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGG

[1563] CTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT

[1564] GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGA

[1565] GTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT

[1566] AAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTC

[1567] ATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCT

[1568] TAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCT

[1569] TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA

[1570] GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTC

[1571] AGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTT CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTG

[1572] CTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT

[1573] AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGA

[1574] ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC

[1575] CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCG

[1576] CACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC

[1577] ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA

[1578] AACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAATTTACACTTTATGCTTCCGGCTCG

[1579] TATGTTGGCCGGCCATGGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTtAAGGAGG

[1580] TcCCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAGACAGCCCATTCCTCAGAGCTT

[1581] TGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATGACCAT

[1582] GCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCC

[1583] ATCCGACACGATCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACA

[1584] AGTATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACAGATTGTATAAGTATG

[1585] CAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAATTGGACAAGAAAATGA

[1586] AGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCCA

[1587] CGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCG

[1588] GTTGACGGACCGACAAGTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTG

[1589] GATAGGCTTTGACACCACCCCTTTTATGTTTAAGAACTTGGCTGGAGCATATCCATCATA

[1590] CTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAGGCCTATGCAGCT

[1591] CTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAAC

[1592] CATCCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTAC

[1593] TGAGGAGCTGGCACCTGCCGTCTGTATTTCACTTACGTGGCAAGCAA

[1594] SEQ ID NO: 7: CAG+T7_VEE_G3_Eukaryotes+Prokaryotes

[1595] A dual expression Self amplifying (SA) vector in Eukaryotes and in Gram negative bacteria like E. coli or Salmonella with updated regulatory sequences. Includes aChicken Beta Actin promoter, Bacterial promoter (TRC) / terminator(T7) / Shine Dalgano ribosomal binding site (SD_RBS), VEE conserved regulatory sequences, and Ploy(A) signal for bacterial and eukaryotic transcriptional termination. This vector has Renilla luciferase as a model gene but it can be replaced by a gene for therapeutic drug genes or antigen genes.

[1596] AACGGCTCGTAACATAGGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTA GAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCATCCAACAATGTTCTATT CTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGC ACCTGCCGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTG AGACTATAGTTAGTTGCGACGGGTACGTCGTTAAAAGAATAGCTATCAGTCCAG GCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGGGATTC TTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTG

[1597] TGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACTGGCATACTGGCAACA GATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCAACCAGCGTAT AGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTT GCCCGTAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATC AAGAAGATGAAAGGCCACTAGGACTACGAGATAGACAGTTAGTCATGGGGTGTT GTTG GG CTTTTAG AAG G CAC AAG ATAACATCTATTTATAAG CG CCCG G ATACCC AAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAG

[1598] G CAGTAACACATTG G AG ATCGG GCTG AG AACAAG AATCAG G AAAATGTTAG AG GAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGGACGTACAAGAAGCTAA GTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCA G CTCTACCACCTTTGG CAG CTG ATGTTG AG G AG CCCACTCTGG AGG CAG ACGT CGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGAGACACCTCGTGGCT TGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTGTG CTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCG CTGAACAAGTCATAGTGATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGG AACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATGCAATACCCGTCCAG GACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTC GTAAACAG GTACCTGCACC ATATTG CCACACATG G AG G AGOG CTG AACACTG AT GAAGAATATTACAAAACTGTCAAGCCCAGCGAGCACGACGGCGAATACCTGTAC GACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAGGGCT CACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGA GAACACGACCAGCCGCTCCTTACCAAGTACCAACCATAGGGGTGTATGGCGTG CCAG G ATCAG GO AAGTCTGG CATCATTAAAAG CG CAGTC ACC AAAAAAG ATCTA GTG GTG AGCG CCAAG AAAG AAAACTGTG CAG AAATTATAAG GG ACGTCAAG AAA ATG AAAG GG CTGG ACGTCAATG CCAG AACTGTG G ACTCAGTG CTCTTG AATGG ATGCAAACACCCCGTAGAGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGC AGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGCTCTG CGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTT TAACCACGAGATTTGCACACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCAC TAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACGACAAAAAAATGAGAACG ACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCT AAGCAGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAA ATAGATTACAAAGGCAACGAAATAATGACGGCAGCTGCCTCTCAAGGGCTGACC CGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTCTGTACGCA CCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGT GTG G AAAACACTAG CCG GCG ACCCATG G ATAAAAACACTG ACTG CCAAGTACC CTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAGCAGAGCATGATGCCATC ATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGC AAACGTGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAG ACATGACCACTGAACAATGGAACACTGTGGATTATTTTGAAACGGACAAAGCTC ACTCAG CAG AG ATAGTATTG AACCAACTATG CGTG AG GTTCTTTG G ACTCG ATC TGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCA CTGGGATAACTCCCCGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCC GTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCAGTTGCCACTGGAAGA GTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTA GTACCTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACAC CC ACAG AGTG ACTTTTCTTCATTCGTCAG CAAATTG AAG GG CAG AACTGTCCTG GTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGTTGTCAGA CCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATG TGCCCAAATATGACATAATATTTGTTAATGTGAGGACCCCATATAAATACCATCA CTATCAG CAGTGTG AAG ACCATG CCATTAAG CTTAG CATGTTG ACCAAG AAAG C TTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGC TGACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTC CCGGGTATGCAAACCGAAATCCTCACTTGAAGAGACGGAAGTTCTGTTTGTATT CATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTACAAGCTTTCATCAAC CTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTC

[1599] ATATCATGTGGTGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAA TGCTGCTAACAGCAAAGGACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATA AGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACA AAGTTTCGGAGGTTGAAGGTGACAAACAGTTGGCAGAGGCTTATGAGTCCATCG CTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTCCACTGTTGTCCAC CGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCT GACAGCTTTAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAA ATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGGAGAGAAGCAGTGGAGGAGA TATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGAGG GTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGG CAAAACTTTCTCATATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGATAT AGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTAT GCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCG AAGAGTCGGAAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCAT GCCATGACTCCAGAAAGAGTACAGCGCCTAAAAGCCTCACGTCCAGAACAAATT ACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGAAG ATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATC CAAGGAAGTATCTCGTGGAAACACCACCGGTAGACGAGACTCCGGAGCCATCG GCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCACCACTTATAACCGA GGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAG AGGATAGCATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTC G AG GCAG ACATTCACG GG COG CCCTCTGTATCTAG CTCATCCTG GTCC ATTCCT CATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGGGA GCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAA GAGTATGGAGTTTCTGGCGCGACCGGTGCCTGCGCCTCGAACAGTATTCAGGA ACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTGCACCCAGCAGG GCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGAT CACTAGAGAGGAGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGG TCTCGAGAACCAGCCTGGTCTCCAACCCGCCAGGCGTAAATAGGGTGATTACA AGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGG TGCATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTA AGGCAAACGGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTGGAGATTTC GTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTACA GTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAA CATGAAAGCCATAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAA GGCAGAAGGAAAAGTGGAGTGCTACCGAACCCTGCATCCTGTTCCTTTGTATTC ATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTAA CGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAG TACGATGCCTATTTGGACATGGTTGACGGAGCTTCATGCTGCTTAGACACTGCC AGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAACACTCCTATTTGGAA CCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGT CCTGGCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCC CGTATTGGATTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATGCGTGTAA TAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAAAC GTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGA AGACACATAATTTGAATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGA CTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAAAACATACTGAAGAACGGCC CAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCG G AATCC ACCG AG AGCTGGTTAG G AG ATTAAATG CG GTCCTG CTTCCG AACATTC ATACACTGTTTGATATGTCGGCTGAAGACTTTGACGCTATTATAGCCGAGCACTT CCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTGATAAAAGTGA GGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGG ACG CAG AGCTGTTG ACG CTG ATTG AG G CGG CTTTCG GCG AAATTTCATCAATAC ATTTGCCCACTAAAACTAAATTTAAATTCGGAGCCATGATGAAATCTGGAATGTT CCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGTTG AGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATC GTG AAAG G AGTC AAATCG G ACAAATTAATGG CAG ACAG GTG CGCCACCTG GTT GAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGAAAGCGCCTTATTT CTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGG

[1600] CAG ACCCCCTAAAAAG G CTGTTTAAGCTTG GCAAACCTCTG GCAG CAG ACG ATG AACATGATGATGACAGGAGAAGGGCATTGCATGAAGAGTCAACACGCTGGAAC CG AGTG G GTATTCTTTCAG AG CTGTG CAAG GCAG TAG AATCAAG GTATGAAACC GTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAAT CATTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACGGCTAACCTGAATGGA CTACGACATatgggcggcgcatgagagaagcccagaccaattacctacccaaaaggaggatataccaatgg agaaagttcacgttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtag aagccaagcaggtcactgatattgaccttgctaaagccagagcgttttcgcatctggctggcagcggcgccacgaact tctctctgttaaagcaagcaggagatgtggaagaaaaccccggtcctGGCGCGCCtGCCACCATGGCT TCCAAGGTGTACGACCCCGAGCAACGCAAACGCATGATCACTGGGCCTCAGTG GTGGGCTCGCTGCAAGCAAATGAACGTGCTGGACTCCTTCATCAACTACTATGA TTCCGAGAAGCACGCCGAGAACGCCGTGATTTTTCTGCATGGTAACGCTGCCTC CAGCTACCTGTGGAGGCACGTCGTGCCTCACATCGAGCCCGTGGCTAGATGCA TCATCCCTGATCTGATCGGAATGGGTAAGTCCGGCAAGAGCGGGAATGGCTCA TATCGCCTCCTGGATCACTACAAGTACCTCACCGCTTGGTTCGAGCTGCTGAAC CTTCCAAAGAAAATCATCTTTGTGGGCCACGACTGGGGGGCTTGTCTGGCCTTT CACTACTCCTACGAGCACCAAGACAAGATCAAGGCCATCGTCCATGCTGAGAGT GTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCTGACATCGAGGAGGATAT CG CCCTG ATCAAG AG CG AAG AG GG CG AG AAAATG GTGCTTG AG AATAACTTCTT CGTCGAGACCATGCTCCCAAGCAAGATCATGCGGAAACTGGAGCCTGAGGAGT TCGCTGCCTACCTGGAGCCATTCAAGGAGAAGGGCGAGGTTAGACGGCCTACC CTCTCCTGGCCTCGCGAGATCCCTCTCGTTAAGGGAGGCAAGCCCGACGTCGT CCAGATTGTCCGCAACTACAACGCCTACCTTCGGGCCAGCGACGATCTGCCTA AGATGTTCATCGAGTCCGACCCTGGGTTCTTTTCCAACGCTATTGTCGAGGGAG CTAAGAAGTTCCCTAACACCGAGTTCGTGAAGGTGAAGGGCCTCCACTTCAGCC AGGAGGACGCTCCAGATGAAATGGGTAAGTACATCAAGAGCTTCGTGGAGCGC GTGCTGAAGAACGAGCAGTAATCTAGAGTGTTTAAACCGACCCGGGCGGCCGC AACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCC CCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTC TATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAAC CTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAG GAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTT GAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCT GGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAA GGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCA AATGG CTCTCCTCAAG CGTATTCAACAAG GG G CTG AAG G ATG CCCAG AAG GTA CCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTT TAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTT CCTTTGAAAAACACGATAATACCGCCACCATGGTGAGCAAGGGCGAGGAGCTG TTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCC ACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCT GACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCC TCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCAC ATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGA GCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGA AGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTC AAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCA CAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAG CAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCT GAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGG TCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTG TACAAGTAG G AATTGG CAAG CTG CTTACATAG AACTCG CG GCG ATTG GCATG CC GCCTTAAAATTTTTATTTTATTTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAA TATTTCAAAAAAAAAAAAAAAAAAAAAAAAAACGCGTtagcataaccccttggggcctctaaac gggtcttgaggggttttttgctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaa ggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggg gtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatggtggggatgcggtgggctct atggCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGG GAAATGTGGGCCGGCCggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaa gagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccg gaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaagg atctgatggcgcaggggatcaagatctgatcaagagacaggatgaggatcgtttcgcatgagccatattcaacggga aacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaa tcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttg ccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatcc gtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctga ttcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagc gatcgtgtatttcgcctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgt aatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggt gatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagacc gataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggt attgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaaTTCGAACTGTCAGACCA AGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGAT CTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTT TCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGAT CCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAG CGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTG GCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAC TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTT CGTG CACACAGCCC AG CTTG G AG CG AACG ACCTACACCG AACTG AG ATACCTA CAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACA GGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCC AGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACT TGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACG CCAGCAACGCGAGCTCGCGATCGCTTAATTAAGCGTTACATAACTTACGGTAAA TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGG AGTATTTACG GTAAACTG CCCACTTG GO AGTACATC AAGTGTATCATATG CCAAG TACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCA GTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATC GCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCC CCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGC GATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGG GGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGG CCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGTTTAGTGAACCGTCAGATCCG CTAGCCTGCAGGttgacaattaatcatccggctcgtataatgtgtggaattATGGGCGGCGCATGAG AGAAGCCCAGACCAATTACCTACCCAAAaggaggatataccaATGGAGAAAGTTCACG TTG ACATCG AGG AAG ACAGCCCATTCCTCAG AG CTTTG CAG CG G AG CTTCCCG CAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATGACCATGCTAATGCCAGA GCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGAC ACG ATCCTTG ACATTG G AAGTGCG CCCG CCCG CAG AATGTATTCTAAGCACAAG TATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACAGATTGTATAAG TATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAATTGGACA AGAAAATGAAGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAG ACTATGTGCCTCCACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGT TTACCAGGATGTATACGCGGTTGACGGACCGACAAGTCTCTATCACCAAGCCAA TAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTT TAAGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAAC CGTGTT

[1601] SEQ ID NO: 8: CAG+T7_VEE_G3_Eukaryotes: expression vector only in eukaryotic cells.

[1602] AACGGCTCGTAACATAGGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGA

[1603] TGTCCATTCTTAGAAAGAAGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTGGCTC

[1604] GACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGTATTTC ACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGG

[1605] TACGTCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCT GCTACGATGCACCGCGAGGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGG

[1606] AGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACT GGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCA

[1607] ACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTAC CTTTTGCCCGTAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCA

[1608] AGAAGATGAAAGGCCACTAGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGG

[1609] CTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCA

[1610] AAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAG ATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCT

[1611] CATTACCGCCGAGGACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTG

[1612] CGTGAAGCCGAGGAGTTGCGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGC

[1613] CCACTCTGGAGGCAGACGTCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGA

[1614] GACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTT

[1615] ACGCTGTGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTC

[1616] TCGCTGAACAAGTCATAGTGATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAA

[1617] CCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCA

[1618] AGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACC TGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTG

[1619] TCAAGCCCAGCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGT CAAGAAAGAACTAGTCACTGGGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTC

[1620] CATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAAC CATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA

[1621] CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGAC

[1622] GTCAAGAAAATGAAAGGGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAA

[1623] TGGATGCAAACACCCCGTAGAGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAG GTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGAT

[1624] CCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATT

[1625] TGCACACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTC

[1626] GTCTCAACCTTGTTTTACGACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATT

[1627] GTGATTGACACTACCGGCAGTACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTC

[1628] AGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGC

[1629] TGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAA

[1630] ATCCTCTGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGAC CGCATCGTGTGGAAAACACTAGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTA

[1631] CCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGA

[1632] GGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGT TGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAAC

[1633] AATGGAACACTGTGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGA

[1634] ACCAACTATGCGTGAGGTTCTTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCA

[1635] CTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACG GGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCA

[1636] GTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCG

[1637] CATAAACCTAGTACCTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGA ACACCCACAGAGTGACTTTTCTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGT

[1638] GGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCT GAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGA

[1639] CATAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGAC CATGCCATTAAGCTTAGCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGG

[1640] AACCTGTGTCAGCATAGGTTATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTG

[1641] CTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAG

[1642] ACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTAC

[1643] AAGCTTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGT

[1644] GCACCCTCATATCATGTGGTGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTAT

[1645] AAATGCTGCTAACAGCAAAGGACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAG

[1646] AAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAA

[1647] AGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGT

[1648] TGAAGGTGACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATA

[1649] ACAATTACAAGTCAGTAGCGATTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAG

[1650] ATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATG

[1651] TAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGG

[1652] AGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGC

[1653] AGAGCTGGTGAGGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACA

[1654] AGCGATGGCAAAACTTTCTCATATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGA

[1655] TATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCA

[1656] TGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCG

[1657] GAAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGA

[1658] AAGAGTACAGCGCCTAAAAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCC

[1659] ATTGCCGAAGTATAGAATCACTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTT

[1660] CTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGG

[1661] TAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACA

[1662] ACCACCACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCG

[1663] AAGAGGAAGAAGAGGATAGCATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCT

[1664] GCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTC

[1665] CTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTA

[1666] GCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGA

[1667] GTTTCTGGCGCGACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCC

[1668] GCTCCGCGCACAAGAACACCGTCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCC

[1669] TAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCT

[1670] TACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCG

[1671] CCAGGCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACA

[1672] ATGACGGTTTGATGCGGGTGCATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTAC

[1673] AACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTG

[1674] GAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTA

[1675] CAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACAT

[1676] GAAAGCCATAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAG

[1677] GAAAAGTGGAGTGCTACCGAACCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACC

[1678] GTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAAC

[1679] TTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACATGGTT

[1680] GACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTT

[1681] TCCAAAGAAACACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCA

[1682] GAACACGCTCCAGAACGTCCTGGCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAA

[1683] TGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATG

[1684] CGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAA

[1685] ACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGA

[1686] CACATAATTTGAATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGA

[1687] GAGACGTGAAAGTGACTCCAGGAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTG

[1688] ATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGG

[1689] TTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG

[1690] AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACT

[1691] GACATCGCGTCGTTTGATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGAT

[1692] TCTGGAAGACTTAGGTGTGGACGCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGC

[1693] GAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAGCCATGATGAAAT

[1694] CTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAG

[1695] TGTTGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATC

[1696] GTGAAAGGAGTCAAATCGGACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATAT

[1697] GGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGT TTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGG CTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAG GGCATTGCATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCA AGGCAGTAGAATCAAGGTATGAAACCGTAGGAACTTCCATCATAGTTATGGCCATGACTA CTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACG GOT AACCT G AAT GG ACT ACGACAT atgggcggcgcatgagagaagcccagaccaattacctacccaaaagga ggatataccaatggagaaagttcacgttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttg aggtagaagccaagcaggtcactgatattgaccttgctaaagccagagcgttttcgcatctggctggcagcggcgccacgaacttc tctctgttaaagcaagcaggagatgtggaagaaaaccccggtcctGGCGCGCCtGCCACCATGGCTTCCAAG GTGTACGACCCCGAGCAACGCAAACGCATGATCACTGGGCCTCAGTGGTGGGCTCGCT GCAAGCAAATGAACGTGCTGGACTCCTTCATCAACTACTATGATTCCGAGAAGCACGCC GAGAACGCCGTGATTTTTCTGCATGGTAACGCTGCCTCCAGCTACCTGTGGAGGCACGT CGTGCCTCACATCGAGCCCGTGGCTAGATGCATCATCCCTGATCTGATCGGAATGGGTA AGTCCGGCAAGAGCGGGAATGGCTCATATCGCCTCCTGGATCACTACAAGTACCTCACC GCTTGGTTCGAGCTGCTGAACCTTCCAAAGAAAATCATCTTTGTGGGCCACGACTGGGG GGCTTGTCTGGCCTTTCACTACTCCTACGAGCACCAAGACAAGATCAAGGCCATCGTCC ATGCTGAGAGTGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCTGACATCGAGGA GGATATCGCCCTGATCAAGAGCGAAGAGGGCGAGAAAATGGTGCTTGAGAATAACTTCT TCGTCGAGACCATGCTCCCAAGCAAGATCATGCGGAAACTGGAGCCTGAGGAGTTCGCT GCCTACCTGGAGCCATTCAAGGAGAAGGGCGAGGTTAGACGGCCTACCCTCTCCTGGC CTCGCGAGATCCCTCTCGTTAAGGGAGGCAAGCCCGACGTCGTCCAGATTGTCCGCAA CTACAACGCCTACCTTCGGGCCAGCGACGATCTGCCTAAGATGTTCATCGAGTCCGACC CTGGGTTCTTTTCCAACGCTATTGTCGAGGGAGCTAAGAAGTTCCCTAACACCGAGTTC GTGAAGGTGAAGGGCCTCCACTTCAGCCAGGAGGACGCTCCAGATGAAATGGGTAAGT ACATCAAGAGCTTCGTGGAGCGCGTGCTGAAGAACGAGCAGTAATCTAGAGTGTTTAAA CCGACCCGGGCGGCCGCAACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCA ATTCCGCCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTG CGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGA AACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGA ATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACA AACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGC CTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTG CCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAA CAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCT CGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAA CCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAATACCGCCACCATGGTGAGCAAG GGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTA AACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAG CTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCG TGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCA GCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCT TCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCT GGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGG CACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA GAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAG CTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCG ACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAG CTGTACAAGTAGGAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGC CTTAAAATTTTTATTTTATTTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAA AAAAAAAAAAAAAAAAAAAAAAAACGCGTtagcataaccccttggggcctctaaacgggtcttgaggggttttttgc tgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaata aaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggatt gggaagacaatagcaggcatggtggggatgcggtgggctctatggCG AGGGGAATT AATT CTT G AAG ACG AA AGGGCC AGGT GGC ACTTTT CGGGGAAAT GT GGGCCGGCCggatgaatgtcagctactgggctatctg gacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttat ggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctt gccgccaaggatctgatggcgcaggggatcaagatctgatcaagagacaggatgaggatcgtttcgcatgagccatattcaacg ggaaacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcag gtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttac agatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggtt actcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggc agtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgcctcgctcaggcgcaatcacgaat gaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaactt ttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgat gttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaac ggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaaTTCGAACTG TCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAG GATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCG TTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTT CTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTT GCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGA TACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAG CACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAT AAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTC GGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAA CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTC CAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAG CGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG CGAGCTCGCGATCGCTTAATTAAGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCT GACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACG CCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTG GCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAA TGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA CATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCAC TCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTT GTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCG GGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGC GGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAA AGCGAAGCGCGCGGCGGGCGGGTTTAGTGAACCGTCAGATCCGCTAGCCTGCAGGTAA TACGACTCACTATAAGATGGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAA AaggaggatataccaATGGAGAAAGTTCACGTTGACATCGAGGAAGACAGCCCATTCCTCAGA GCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATGA CCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGG ACCCATCCGACACGATCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAG CACAAGTATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACAGATTGTATAAG TATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAATTGGACAAGAAA ATGAAGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCC TCCACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATAC GCGGTTGACGGACCGACAAGTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTA CTGGATAGGCTTTGACACCACCCCTTTTATGTTTAAGAACTTGGCTGGAGCATATCCATC ATACTCTACCAACTGGGCCGACGAAACCGTGTT

[1698] SEQ ID NO: 9 TRC_VEE_G3_Prokaryotes: only expressed in gram negative bacteria

[1699] AACGGCTCGTAACATAGGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGA

[1700] TGTCCATTCTTAGAAAGAAGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTGGCTC

[1701] GACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGTATTTC

[1702] ACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGG

[1703] TACGTCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCT

[1704] GCTACGATGCACCGCGAGGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGG

[1705] AGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACT

[1706] GGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCA

[1707] ACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTAC CTTTTGCCCGTAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCA

[1708] AGAAGATGAAAGGCCACTAGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGG

[1709] CTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCA

[1710] AAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAG

[1711] ATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCT

[1712] CATTACCGCCGAGGACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTG

[1713] CGTGAAGCCGAGGAGTTGCGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGC

[1714] CCACTCTGGAGGCAGACGTCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGA

[1715] GACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTT

[1716] ACGCTGTGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTC

[1717] TCGCTGAACAAGTCATAGTGATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAA

[1718] CCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCA

[1719] AGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACC

[1720] TGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTG

[1721] TCAAGCCCAGCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGT

[1722] CAAGAAAGAACTAGTCACTGGGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTC

[1723] CATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAAC

[1724] CATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA

[1725] CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGAC

[1726] GTCAAGAAAATGAAAGGGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAA

[1727] TGGATGCAAACACCCCGTAGAGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAG

[1728] GTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGAT

[1729] CCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATT

[1730] TGCACACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTC

[1731] GTCTCAACCTTGTTTTACGACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATT

[1732] GTGATTGACACTACCGGCAGTACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTC

[1733] AGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGC

[1734] TGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAA

[1735] ATCCTCTGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGAC

[1736] CGCATCGTGTGGAAAACACTAGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTA

[1737] CCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGA

[1738] GGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGT

[1739] TGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAAC

[1740] AATGGAACACTGTGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGA

[1741] ACCAACTATGCGTGAGGTTCTTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCA

[1742] CTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACG

[1743] GGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCA

[1744] GTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCG

[1745] CATAAACCTAGTACCTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGA

[1746] ACACCCACAGAGTGACTTTTCTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGT

[1747] GGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCT

[1748] GAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGA

[1749] CATAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGAC

[1750] CATGCCATTAAGCTTAGCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGG

[1751] AACCTGTGTCAGCATAGGTTATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTG

[1752] CTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAG

[1753] ACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTAC

[1754] AAGCTTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGT

[1755] GCACCCTCATATCATGTGGTGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTAT

[1756] AAATGCTGCTAACAGCAAAGGACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAG

[1757] AAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAA

[1758] AGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGT

[1759] TGAAGGTGACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATA

[1760] ACAATTACAAGTCAGTAGCGATTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAG

[1761] ATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATG

[1762] TAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGG

[1763] AGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGC

[1764] AGAGCTGGTGAGGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACA

[1765] AGCGATGGCAAAACTTTCTCATATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGA TATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCA TGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCG GAAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGA AAGAGTACAGCGCCTAAAAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCC ATTGCCGAAGTATAGAATCACTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTT CTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGG TAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACA ACCACCACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCG AAGAGGAAGAAGAGGATAGCATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCT GCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTC CTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTA GCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGA

[1766] GTTTCTGGCGCGACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCC GCTCCGCGCACAAGAACACCGTCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCC TAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCT TACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCG CCAGGCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACA ATGACGGTTTGATGCGGGTGCATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTAC AACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTG GAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTA CAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACAT GAAAGCCATAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAG GAAAAGTGGAGTGCTACCGAACCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACC GTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAAC

[1767] TTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACATGGTT GACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTT TCCAAAGAAACACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCA GAACACGCTCCAGAACGTCCTGGCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAA TGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATG CGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAA ACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGA CACATAATTTGAATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGA GAGACGTGAAAGTGACTCCAGGAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTG ATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGG TTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACT

[1768] GACATCGCGTCGTTTGATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGAT TCTGGAAGACTTAGGTGTGGACGCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGC GAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAGCCATGATGAAAT CTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAG TGTTGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATC GTGAAAGGAGTCAAATCGGACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATAT GGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGT TTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGG CTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAG GGCATTGCATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCA AGGCAGTAGAATCAAGGTATGAAACCGTAGGAACTTCCATCATAGTTATGGCCATGACTA CTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACG

[1769] GOT AACCT G AAT GG ACT ACGACAT atgggcggcgcatgagagaagcccagaccaattacctacccaaaagga ggatataccaatggagaaagttcacgttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttg aggtagaagccaagcaggtcactgatattgaccttgctaaagccagagcgttttcgcatctggctggcagcggcgccacgaacttc tctctgttaaagcaagcaggagatgtggaagaaaaccccggtcctGGCGCGCCtGCCACCATGGCTTCCAAG GTGTACGACCCCGAGCAACGCAAACGCATGATCACTGGGCCTCAGTGGTGGGCTCGCT GCAAGCAAATGAACGTGCTGGACTCCTTCATCAACTACTATGATTCCGAGAAGCACGCC GAGAACGCCGTGATTTTTCTGCATGGTAACGCTGCCTCCAGCTACCTGTGGAGGCACGT CGTGCCTCACATCGAGCCCGTGGCTAGATGCATCATCCCTGATCTGATCGGAATGGGTA AGTCCGGCAAGAGCGGGAATGGCTCATATCGCCTCCTGGATCACTACAAGTACCTCACC GCTTGGTTCGAGCTGCTGAACCTTCCAAAGAAAATCATCTTTGTGGGCCACGACTGGGG GGCTTGTCTGGCCTTTCACTACTCCTACGAGCACCAAGACAAGATCAAGGCCATCGTCC ATGCTGAGAGTGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCTGACATCGAGGA GGATATCGCCCTGATCAAGAGCGAAGAGGGCGAGAAAATGGTGCTTGAGAATAACTTCT TCGTCGAGACCATGCTCCCAAGCAAGATCATGCGGAAACTGGAGCCTGAGGAGTTCGCT GCCTACCTGGAGCCATTCAAGGAGAAGGGCGAGGTTAGACGGCCTACCCTCTCCTGGC CTCGCGAGATCCCTCTCGTTAAGGGAGGCAAGCCCGACGTCGTCCAGATTGTCCGCAA CTACAACGCCTACCTTCGGGCCAGCGACGATCTGCCTAAGATGTTCATCGAGTCCGACC CTGGGTTCTTTTCCAACGCTATTGTCGAGGGAGCTAAGAAGTTCCCTAACACCGAGTTC GTGAAGGTGAAGGGCCTCCACTTCAGCCAGGAGGACGCTCCAGATGAAATGGGTAAGT ACATCAAGAGCTTCGTGGAGCGCGTGCTGAAGAACGAGCAGTAATCTAGAGTGTTTAAA CCGACCCGGGCGGCCGCAACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCA ATTCCGCCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTG CGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGA AACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGA ATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACA AACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGC CTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTG CCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAA CAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCT CGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAA CCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAATACCaggaggatataccaATGGTGAG CAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGA CGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGG CAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC CTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCAT CTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGAC

[1770] ACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCC TGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAG CAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG TGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCT GCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAG CGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGG ACGAGCTGTACAAGTAGGAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCAT GCCGCCTTAAAATTTTTATTTTATTTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATA TTTCAAAAAAAAAAAAAAAAAAAAAAAAAACGCGTtagcataaccccttggggcctctaaacgggtcttgag gggtttttgCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAA ATGTGGGCCGGCCggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcag gtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgc cctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagatct gatcaagagacaggatgaggatcgtttcgcatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatggat gctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcg ccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaattt atgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattcca ggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaatt gtccttttaacagcgatcgtgtatttcgcctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacg agcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgat ttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggat cttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaat aaattgcagttcattgatgctcgatgagttttctaaTTCGAACTGTCAGACCAAGTTTACTCATATATACTTT AGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAAT CTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAA AAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA

[1771] AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCG TAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATC CTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAG ACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAG CCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGA AAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGT CGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGT CCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGG GCGGAGCCTATGGAAAAACGCCAGCAACGCGAGCTCGCGATCGCTTAATTAAttgacaattaa tcatccggctcgtataatgtgtggaattATGGGCGGCGCATGAGAGAAGCCCAGACCAATT ACCT AGCO AAAaggaggatataccaATGGAGAAAGTTCACGTTGACATCGAGGAAGACAGCCCATTCCTCA GAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAAT GACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGT GGACCCATCCGACACGATCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTA AGCACAAGTATCATTGTATCTGTCCGATGAGATGTGCGGAAGATCCGGACAGATTGTATA AGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGGAATTGGACAAGA AAATGAAGGAGCTGGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTG CCTCCACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTAT ACGCGGTTGACGGACCGACAAGTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCC TACTGGATAGGCTTTGACACCACCCCTTTTATGTTTAAGAACTTGGCTGGAGCATATCCA

[1772] TCATACTCTACCAACTGGGCCGACGAAACCGTGTT

[1773] SEQ ID NO: 10: CMV+T7_VEE_OmicronS_C2 DNA

[1774] AACGGCTCGTAACATAGGCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGA

[1775] TGTCCATTCTTAGAAAGAAGTATTTGAAACCATCCAACAATGTTCTATTCTCTGTTGGCTC

[1776] GACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGCCGTCTGTATTTC

[1777] ACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGG

[1778] TACGTCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCT

[1779] GCTACGATGCACCGCGAGGGATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGG

[1780] AGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAGCTACATTGTGTGACCAAATGACT

[1781] GGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTGGGCTCA

[1782] ACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTAC

[1783] CTTTTGCCCGTAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCA

[1784] AGAAGATGAAAGGCCACTAGGACTACGAGATAGACAGTTAGTCATGGGGTGTTGTTGGG

[1785] CTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGGATACCCAAACCATCATCA

[1786] AAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGGAG

[1787] ATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCT

[1788] CATTACCGCCGAGGACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTG

[1789] CGTGAAGCCGAGGAGTTGCGCGCAGCTCTACCACCTTTGGCAGCTGATGTTGAGGAGC

[1790] CCACTCTGGAGGCAGACGTCGACTTGATGTTACAAGAGGCTGGGGCCGGCTCAGTGGA

[1791] GACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTT

[1792] ACGCTGTGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTC

[1793] TCGCTGAACAAGTCATAGTGATAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAA

[1794] CCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATGCAATACCCGTCCAGGACTTTCA

[1795] AGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACAGGTACC

[1796] TGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTG

[1797] TCAAGCCCAGCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGT

[1798] CAAGAAAGAACTAGTCACTGGGCTAGGGCTCACAGGCGAGCTGGTGGATCCTCCCTTC

[1799] CATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTCCTTACCAAGTACCAAC

[1800] CATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA

[1801] CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGAC

[1802] GTCAAGAAAATGAAAGGGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAA

[1803] TGGATGCAAACACCCCGTAGAGACCCTGTATATTGACGAAGCTTTTGCTTGTCATGCAG

[1804] GTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGCTCTGCGGGGAT

[1805] CCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATT

[1806] TGCACACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTC

[1807] GTCTCAACCTTGTTTTACGACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATT

[1808] GTGATTGACACTACCGGCAGTACCAAACCTAAGCAGGACGATCTCATTCTCACTTGTTTC

[1809] AGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAATGACGGCAGC

[1810] TGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAA

[1811] ATCCTCTGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGAC

[1812] CGCATCGTGTGGAAAACACTAGCCGGCGACCCATGGATAAAAACACTGACTGCCAAGTA

[1813] CCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAGCAGAGCATGATGCCATCATGA

[1814] GGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACGTGTGT TGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAAC

[1815] AATGGAACACTGTGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGA

[1816] ACCAACTATGCGTGAGGTTCTTTGGACTCGATCTGGACTCCGGTCTATTTTCTGCACCCA

[1817] CTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCCCGTCGCCTAACATGTACG

[1818] GGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGGCA

[1819] GTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCG

[1820] CATAAACCTAGTACCTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGA

[1821] ACACCCACAGAGTGACTTTTCTTCATTCGTCAGCAAATTGAAGGGCAGAACTGTCCTGGT

[1822] GGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGTTGTCAGACCGGCCT

[1823] GAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGA

[1824] CATAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGAC

[1825] CATGCCATTAAGCTTAGCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGG

[1826] AACCTGTGTCAGCATAGGTTATGGTTACGCTGACAGGGCCAGCGAAAGCATCATTGGTG

[1827] CTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCTCACTTGAAGAG

[1828] ACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATTCTTAC

[1829] AAGCTTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGT

[1830] GCACCCTCATATCATGTGGTGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTAT

[1831] AAATGCTGCTAACAGCAAAGGACAACCTGGCGGAGGGGTGTGCGGAGCGCTGTATAAG

[1832] AAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGACTGGTCAA

[1833] AGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGT

[1834] TGAAGGTGACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATA

[1835] ACAATTACAAGTCAGTAGCGATTCCACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAG

[1836] ATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTTTAGACACCACTGATGCAGATG

[1837] TAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGGCTAGG

[1838] AGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGC

[1839] AGAGCTGGTGAGGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACA

[1840] AGCGATGGCAAAACTTTCTCATATTTGGAAGGGACCAAGTTTCACCAGGCGGCCAAGGA

[1841] TATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCAATGAGCAGGTATGCA

[1842] TGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCG

[1843] GAAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGA

[1844] AAGAGTACAGCGCCTAAAAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCC

[1845] ATTGCCGAAGTATAGAATCACTGGTGTGCAGAAGATCCAATGCTCCCAGCCTATATTGTT

[1846] CTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGGAAACACCACCGG

[1847] TAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACA

[1848] ACCACCACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCG

[1849] AAGAGGAAGAAGAGGATAGCATAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCT

[1850] GCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTATCTAGCTCATCCTGGTCCATTC

[1851] CTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGGGAGCTA

[1852] GCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGA

[1853] GTTTCTGGCGCGACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCC

[1854] GCTCCGCGCACAAGAACACCGTCACTTGCACCCAGCAGGGCCTGCTCGAGAACCAGCC

[1855] TAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGGAGCTCGAGGCGCT

[1856] TACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCG

[1857] CCAGGCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACA

[1858] ATGACGGTTTGATGCGGGTGCATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTAC

[1859] AACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAGTGGTGTTGGAGAGGACCGAATTG

[1860] GAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCAAGAAATTA

[1861] CAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACAT

[1862] GAAAGCCATAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAG

[1863] GAAAAGTGGAGTGCTACCGAACCCTGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACC

[1864] GTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTAACGCCATGTTGAAAGAGAAC

[1865] TTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACATGGTT

[1866] GACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTT

[1867] TCCAAAGAAACACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCA

[1868] GAACACGCTCCAGAACGTCCTGGCAGCTGCCACAAAAAGAAATTGCAATGTCACGCAAA

[1869] TGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGGAATGCTTCAAGAAATATG

[1870] CGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAGAAA

[1871] ACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGA

[1872] CACATAATTTGAATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGA GAGACGTGAAAGTGACTCCAGGAACAAAACATACTGAAGAACGGCCCAAGGTACAGGTG

[1873] ATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAATCCACCGAGAGCTGG

[1874] TTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG

[1875] AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACT

[1876] GACATCGCGTCGTTTGATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGAT

[1877] TCTGGAAGACTTAGGTGTGGACGCAGAGCTGTTGACGCTGATTGAGGCGGCTTTCGGC

[1878] GAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAGCCATGATGAAAT

[1879] CTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAG

[1880] TGTTGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATC

[1881] GTGAAAGGAGTCAAATCGGACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATAT

[1882] GGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGAAAGCGCCTTATTTCTGTGGAGGGT

[1883] TTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCCTAAAAAGG

[1884] CTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAG

[1885] GGCATTGCATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCA

[1886] AGGCAGTAGAATCAAGGTATGAAACCGTAGGAACTTCCATCATAGTTATGGCCATGACTA

[1887] CTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAGGGGCCCCTATAACTCTCTACG

[1888] GCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAGTCTGTTTCTAGAGCCACCAT

[1889] GTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTTACAACCA

[1890] GGACCCAGCTGCCTCCCGCCTACACCAACAGCTTCACAAGGGGCGTTTACTACCCCGA

[1891] CAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCA

[1892] GCAACGTGACCTGGTTCCACGTGATCAGCGGCACCAACGGCACCAAGAGGTTCGACAA

[1893] CCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCATCGAGAAGAGCAACATCA

[1894] TCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAGCCTTCTGATCGT

[1895] GAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCT

[1896] TCCTGGACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAG

[1897] CGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGC

[1898] AAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

[1899] CAAGATCTACAGCAAGCACACCCCTATCATCGTGAGGGAGCCCGAGGACCTGCCTCAG

[1900] GGATTCTCTGCCCTGGAACCTTTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTT

[1901] CCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACACCTGGAGACAGCAGCTCTGGC

[1902] TGGACAGCTGGAGCCGCTGCTTACTACGTGGGCTACCTGCAACCTAGGACCTTCCTGCT

[1903] GAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTTGATCCTCTG

[1904] TCTGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCA

[1905] GCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCT

[1906] GTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCTTGGAAC

[1907] AGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACCTGGCCCCTT

[1908] TCTTCACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACC

[1909] AACGTGTACGCCGACAGCTTCGTGATCAGGGGCGATGAAGTGAGGCAGATCGCCCCTG

[1910] GCCAGACCGGAAACATCGCTGACTACAATTACAAGCTGCCCGACGACTTCACCGGCTGC

[1911] GTGATCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGAGCGGCAACTATAACTACCT

[1912] GTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAG

[1913] ATCTACCAGGCCGGCAACAAGCCCTGTAATGGAGTGGCCGGCTTCAACTGCTACTTCCC

[1914] CTTGAGGAGCTACAGCTTCAGGCCCACCTACGGCGTTGGACACCAGCCTTACAGGGTG

[1915] GTGGTGCTGTCTTTCGAGCTGCTGCATGCCCCTGCCACCGTTTGCGGACCCAAGAAGA

[1916] GCACCAACCTGGTGAAGAACAAGTGCGTGAATTTCAACTTCAATGGCCTGAAGGGCACC

[1917] GGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACA

[1918] TCGCCGACACCACAGATGCTGTGAGGGACCCTCAGACACTGGAGATCCTGGACATCAC

[1919] CCCTTGCAGCTTCGGCGGCGTGTCCGTGATTACCCCTGGCACCAACACAAGCAACCAG

[1920] GTGGCCGTGCTGTACCAGGGCGTTAACTGCACCGAGGTGCCTGTTGCCATCCACGCCG

[1921] ACCAGTTAACCCCTACCTGGAGGGTGTACAGCACCGGCAGCAACGTTTTCCAGACAAGG

[1922] GCCGGCTGCCTGATTGGCGCTGAGTACGTGAACAACAGCTACGAGTGCGACATCCCCA

[1923] TCGGCGCCGGCATCTGCGCCTCTTATCAAACCCAGACCAAGAGCCACAGCGTGGCCAG

[1924] CCAAAGCATTATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGC

[1925] AACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

[1926] CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACAGCACC

[1927] GAGTGCAGCAACCTGCTGCTGCAGTACGGAAGCTTCTGCACCCAGCTGAAGAGGGCCC

[1928] TGACCGGCATTGCCGTTGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

[1929] GCAGATCTACAAGACCCCTCCCATCCCCCCCTTCGGCGGCTTCAACTTCAGCCAGATCC

[1930] TGCCCGACCCCAGCAAGCCCTCTAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGAGACTGCTTGGGAGACATCG

[1931] CCGCCAGGGACCTGATCTGCGCTCAGAAGTTCAAGGGCCTGACCGTGTTGCCTCCCCT

[1932] GCTGACCGACGAGATGATCGCTCAGTACACCAGCGCCCTGTTGGCCGGCACCATTACAT

[1933] CTGGCTGGACATTTGGAGCCGGCGCCGCCTTACAGATCCCCTTTGCCATGCAGATGGCT

[1934] TACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGAT

[1935] CGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGATAGCCTGAGCAGCACCGCT

[1936] TCTGCCCTGGGCAAGCTGCAGGATGTGGTGAACCACAACGCCCAGGCCCTTAACACCC

[1937] TGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAGCGTGCTGAACGACATCTT

[1938] CAGCAGGCTGGACAAGGTGGAGGCCGAGGTGCAGATCGACAGGCTGATCACAGGCAG

[1939] GCTGCAGAGCTTGCAGACCTACGTGACCCAGCAGCTGATTAGGGCCGCCGAGATCAGG

[1940] GCCTCTGCTAACCTGGCTGCCACCAAGATGTCTGAGTGCGTGCTGGGCCAGAGCAAGA

[1941] GAGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGCTTCCCTCAGAGCGCCCCTCA

[1942] TGGAGTGGTGTTCCTGCACGTGACCTACGTGCCTGCTCAGGAGAAGAACTTCACCACCG

[1943] CCCCTGCCATCTGCCACGACGGCAAGGCTCATTTTCCTAGGGAGGGAGTGTTCGTGAG

[1944] CAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCA

[1945] CCGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACAC

[1946] CGTGTACGACCCTCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTAC

[1947] TTCAAGAACCACACCAGCCCCGACGTGGACCTCGGCGACATCAGCGGAATTAACGCTA

[1948] GCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAA

[1949] CGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCC

[1950] TGGTACATCTGGCTGGGCTTCATCGCCGGCCTTATCGCCATCGTGATGGTGACCATCAT

[1951] GCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCTCTTGTGGCAGC

[1952] TGCTGTAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACT

[1953] ACACCGGTTCTTCTGAAAACCTGTACTTCCAATCCAAATCTTCTCACCATCACCATCACCA

[1954] TCACCATCACCATTAGAAACCGACCCGGGCGGCCGCAACTAACTTAAGCTAGCAACGGT

[1955] TTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTT

[1956] GGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGG

[1957] CAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTT

[1958] CCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTG

[1959] GAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCC

[1960] ACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAG

[1961] GCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCT

[1962] CTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGG

[1963] GATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAAC

[1964] GTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAATACCAT

[1965] GACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGT

[1966] ACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCG

[1967] GACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGC

[1968] TCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCA

[1969] CGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCC

[1970] GAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCG

[1971] CACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCAC

[1972] CAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCG

[1973] CGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAG

[1974] CGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGG

[1975] TGCATGACCCGCAAGCCCGGTGCCTGAGAATTGGCAAGCTGCTTACATAGAACTCGCG

[1976] GCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTTCTTTTCTTTTCCGAATCGGATT

[1977] TTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAACGCGTCGAGGGGAATTAAT

[1978] TCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT

[1979] TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAAT

[1980] GCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT

[1981] CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA

[1982] AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAG

[1983] CGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAA

[1984] AGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTC

[1985] GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATC

[1986] TTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACA

[1987] CTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG

[1988] CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGC CATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCA

[1989] AACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGG

[1990] AGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT

[1991] GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGC

[1992] CAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG

[1993] GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT

[1994] GTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAA

[1995] GGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTC

[1996] GTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT

[1997] TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTT

[1998] GCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGA

[1999] TACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAG

[2000] CACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAT

[2001] AAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTC

[2002] GGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAA

[2003] CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG

[2004] CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTC

[2005] CAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAG

[2006] CGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

[2007] CGAGCTCGCGATCGCTTAATTAACGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

[2008] ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGC

[2009] CAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGG

[2010] CAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAAT

[2011] GGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTAC

[2012] ATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGG

[2013] CGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGG

[2014] GAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCC

[2015] ATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGT

[2016] TTAGTGAACCGTCAGATCCGCTAGTAATACGACTCACTATAGGGCCGGCCATGGGCGGC

[2017] GCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATC

[2018] GAGGAAGACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGA

[2019] AGCCAAGCAGGTCACTGATAATGACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTT

[2020] CAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGATCCTTGACATTGGAAGTGCG

[2021] CCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGATGTGCG

[2022] GAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATA

[2023] ACTGATAAGGAATTGGACAAGAAAATGAAGGAGCTGGCCGCCGTCATGAGCGACCCTGA

[2024] CCTGGAAACTGAGACTATGTGCCTCCACGACGACGAGTCGTGTCGCTACGAAGGGCAA

[2025] GTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAAGTCTCTATCACCAAGC

[2026] CAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTAA

[2027] GAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTT

[2028] Although the invention has been described with reference to certain specific embodiments, various modifications thereof will be apparent to those skilled in the art without departing from the spirit and scope of the invention. All such modifications as would be apparent to one skilled in the art are intended to be included within the scope of the following claims.

Claims

WE CLAIM:

1. An attenuated Salmonella bacteria comprising a self-amplifying DNA vector encoding one or more payloads, optionally wherein said self-amplifying DNA vector is capable of expressing said one or more payloads (a) only when delivered to a eukaryotic cell; (b) only in said bacteria; or (c) in an eukaryotic cell and said bacteria.

2. The bacteria of claim 1 or 2, wherein said self-amplifying DNA vector comprises: one or more promoters; replicon protein genes from Venezuelan equine encephalitis (VEE) virus under the control of the promoter; a sub-genomic promoter from the VEE virus, a nucleic acid encoding a payload under the control of the sub-genomic promoter.

3. A pharmaceutical composition comprising the Salmonella bacteria of claim 1 or 2 and a pharmaceutically acceptable carrier.

4. The pharmaceutical composition of claim 3, wherein said pharmaceutical composition is formulated for oral administration.

5. A method of treating, and / or preventing disease in a subject, said method comprising orally administering one or more of the bacteria of claim 1 or 2, wherein said vector expresses a therapeutic polypeptide effective against said disease, optionally wherein each of said one or more of the bacteria express one or more different payloads; and optionally in combination with a further therapeutic.

6. A method of stimulating an antigen-specific immune response, said method comprising administering said method comprising orally administering the bacteria of claim 1 or 2, wherein said vector expresses one or more immunogens or epitopes from an infectious agent or cancer cell expressing the antigen.

7. An attenuated Gram-negative bacteria comprising a self-amplifying nucleic acid vector encoding one or more payloads, optionally wherein said self-amplifying nucleic acid vector is capable of expressing said one or more payloads:a) only when delivered to a eukaryotic cell; b) only in said bacteria; or c) in a eukaryotic cell and said bacteria.

8. The bacteria of claim 1 , wherein said Gram-negative bacteria is selected from Salmonella, Escherichia coli, Shigella, Yersinia, or Pseudomonas.

9. The bacteria of claim 7 or 8, wherein said self-amplifying nucleic acid vector comprises: a) one or more promoters; b) a replicon system enabling autonomous amplification of said nucleic acid within a eukaryotic cell and / or bacteria; c) a sub-genomic promoter for controlled expression; d) a nucleic acid payload of up to 20kb under the control of said sub-genomic promoter.

10. The bacteria of claim 3, wherein said self-amplifying nucleic acid vector comprises: a) a self-amplifying RNA vector system; or b) a self-amplifying DNA vector system.

11. A pharmaceutical composition comprising the bacteria of any one of claims 7 to 10 and a pharmaceutically acceptable carrier.

12. A method of treating, and / or preventing disease in a subject, said method comprising administering one or more of the bacteria of any one of claims 7 to 10, wherein said vector expresses a therapeutic polypeptide effective against said disease, optionally wherein each of said one or more of the bacteria express one or more different payloads; and optionally in combination with a further therapeutic.

13. The method of claim 12, wherein said administering comprises: oral delivery; intravenous injection; intranasal administration; intramuscular injection; and / or sublingual administration.

14. The method of claim 12, wherein the bacteria are administered in a single dose, multiple doses, or a time-course regimen.

15. The method of claim 14, wherein the dose is administered at regular intervals, optionally wherein the dosing schedule is selected based on the therapeutic need and the pharmacokinetics of the bacteria.

16. The method of claim 15, wherein the time-course of administration involves a series of escalating doses, optionally with increased frequency or dosage over time to achieve optimal therapeutic effects.

17. The method of claim 29, wherein the subject is a mammal, optionally a human, a reptile, a bird, an amphibian, or a fish.

18. The bacteria of claim 9, wherein said replicon system is derived from a virus selected from a) Alphaviruses (Venezuelan equine encephalitis virus (VEEV), Sindbis virus, Semliki Forest virus); b) Flaviviruses (Dengue virus, Zika virus, Yellow Fever virus); c) Paramyxoviruses (Sendai virus, Measles virus); and d) Rhabdoviruses (Vesicular stomatitis virus, Rabies virus).