Evaluation of mRNA transcript levels using DDPCR for the assessment of early cell populations and single-cell clones in cell line development

CL202601233A1Pending Publication Date: 2026-07-24AMGEN INC
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Patent Information

Application Number
CL202601233
Authority / Receiving Office
CL · CL
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-10-17
Filing Date
2026-04-16
Publication Date
2026-07-24
Patent Text Reader

Abstract

This disclosure relates to the field of cell culture technology. It provides a method for facilitating the selection of a single-cell clone or cell populations for the production of an antigen-binding protein with two to four different antibody chains. The method uses ddPCR for the quantification of transcription levels.
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