固定化赖氨酸脱羧酶、其制备、1,5-戊二胺制备方法及产品

The immobilization method, which involves forming covalent bonds between an aldehyde-modified polymer carrier and lysine decarboxylase, solves the problems of low stability and reusability of immobilized lysine decarboxylase, enabling low-cost and high-efficiency production of 1,5-pentanediamine and promoting the industrialization of biological production.

CN107779447BActive Publication Date: 2026-07-17CATHAY BIOTECH INC +2

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CATHAY BIOTECH INC
Filing Date
2016-08-25
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In the existing technology, the stability and reusability of immobilized lysine decarboxylase are low, resulting in high production costs of 1,5-pentanediamine. In addition, the cost of carrier materials is high, and the immobilization method is complex, making it difficult to achieve industrial production.

Method used

An immobilization method using aldehyde-modified polymeric carriers to form covalent bonds with lysine decarboxylase was developed. Stable immobilized lysine decarboxylase was prepared by reacting low-cost polymeric materials such as cellulose and polyvinyl alcohol with aldehyde-modifying reagents. This immobilized lysine decarboxylase was used to catalyze the decarboxylation reaction of lysine to produce 1,5-pentanediamine.

Benefits of technology

It improves enzyme stability and reusability, reduces production costs, simplifies the preparation process of 1,5-pentanediamine, and promotes the industrialization of biological production.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

本发明涉及固定化赖氨酸脱羧酶、其制备及1,5‑戊二胺制备方法及制得的1,5‑戊二胺。具体涉及一种包括醛基修饰的高分子载体和赖氨酸脱羧酶的固定化赖氨酸脱羧酶,及以醛基修饰的高分子载体制备固定化赖氨酸脱羧酶的方法;及以固定化赖氨酸脱羧酶制备1,5‑戊二胺的方法及其制得的1,5‑戊二胺。该固定化方法对酶的固定化效率高,使用稳定性好,提高酶的使用效率,解决传统工艺中游离态赖氨酸脱羧酶(细胞)使用稳定性差的问题,极大降低载体的使用成本和1,5‑戊二胺生物法生产成本,简化1,5‑戊二胺溶液和酶的分离步骤,加大1,5‑戊二胺生产的自动化程度,推进了生物法生产1,5‑戊二胺的产业化进程。
Need to check novelty before this filing date? Find Prior Art