一种核酸测序方法、反应体系和试剂盒
By using ddNTPs to terminate the sequencing reaction at different positions on the nucleic acid template, the problems of short read lengths and resource waste in high-throughput sequencing are solved, enabling the reading of longer sequence fragments and simultaneous sequencing of multiple samples, thus improving sequencing quality and efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- MGI TECH CO LTD
- Filing Date
- 2017-01-18
- Publication Date
- 2026-07-17
AI Technical Summary
Existing high-throughput sequencing technologies suffer from problems such as short read lengths and data waste, especially in next-generation sequencing, where short read lengths lead to low sequence assembly efficiency and insufficient resource utilization when sequencing multiple samples.
Dideoxynucleotides (ddNTPs) are used to sequence at different positions on the nucleic acid template. Sequencing is blocked by a termination reaction, and excess ddNTPs are removed by elution reagents to achieve multi-segment sequencing of the same nucleic acid template. ddNTPs are used to ligate the ends of the generated sequencing strand to block the first segment of sequencing, and new primers are used to hybridize at other positions to perform the next segment of sequencing.
The sequencing read length was increased, the amount of data was increased, and simultaneous sequencing of multiple samples was achieved, thereby improving resource utilization and sequencing quality.
Smart Images
Figure CN108330186B_ABST