Orchid extract with antifungal function, composition thereof and use thereof

Abstract

An orchid extract includes at least one anti-fungal component, and the extract is extracted from a part of an orchid, and the anti-fungal component has an anti-fungal activity. An orchid extract additive product is to add the orchid extract into a raw material to make an anti-fungal composition.

Description

Technical Field

[0001] This invention relates to an orchid extract and composition thereof with antifungal function; particularly to an orchid seed or protocorm extract and composition thereof with antifungal function; and even more particularly to an orchid extract and composition thereof containing vicilin protein with antifungal function. Background Technology

[0002] Existing orchid extracts, such as the invention patent application No. 201318647 of Taiwan, entitled "Extraction Method and Application of Orchid Extract," disclose an orchid extract and its extraction method. The extraction method includes: step 1, extracting an orchid using water or methanol to obtain an orchid aqueous extract or an orchid methanol extract; step 2, adding ethyl acetate and water to the orchid methanol extract obtained in step 1 for extraction to obtain an orchid ethyl acetate extract and an orchid aqueous extract; and step 3, adding n-butanol and water to the orchid aqueous extract obtained in step 2 for extraction to obtain an orchid n-butanol extract.

[0003] As mentioned above, the orchid water extract, orchid methanol extract, orchid ethyl acetate extract, or orchid n-butanol extract of patent No. 201318647 exhibits inhibitory activity against tyrosinase. Furthermore, these orchid water extracts, orchid methanol extracts, orchid ethyl acetate extracts, or orchid n-butanol extracts are effective in inhibiting LPS (lipopolysaccharide)-induced inflammatory responses.

[0004] In fact, the aforementioned Publication No. 201318647 only discloses that orchid water extract, orchid methanol extract, orchid ethyl acetate extract, or orchid n-butanol extract can inhibit tyrosinase activity and inflammatory responses. Therefore, existing orchid extraction technologies still need to be further developed to achieve other related effects using orchid extracts, in order to extract extracts with antifungal, skin-care, and antioxidant effects.

[0005] Another existing seed extract, such as the invention patent application No. 201427674 of Taiwan, entitled "Phaseolus vulgaris seed extract and its preparation method and use," discloses a seed extract of kidney bean (Phaseolus vulgaris L.) and its preparation method. The extraction method includes: step a, grinding the kidney bean seeds to obtain a kidney bean powder; step b, dissolving the kidney bean powder in a first solvent to obtain a homogeneous kidney bean extract; and step c, centrifuging the homogeneous kidney bean extract and collecting a supernatant kidney bean extract. Step b is performed at a ratio of approximately 1 gram of kidney bean powder dissolved in 10 ml of the first solvent.

[0006] Continuing on the above, the first solvent is selected from water, C1-C4 alcohols, and any combination thereof. Alternatively, the first solvent is a 50% aqueous ethanol solution (v / v), and the aqueous ethanol solution is about 40% to about 60% by volume. Alternatively, the first solvent is an 80% aqueous methanol solution (v / v), and the aqueous methanol solution is about 70% to about 90% by volume. In fact, the aforementioned Publication No. 201427674 only discloses the relevant technology of kidney bean seed extract and its preparation method and uses. In fact, existing related seed extraction technologies still have the need to further explore other suitable seeds in order to extract extracts with antifungal, skin care, and antioxidant effects.

[0007] Another existing orchid extraction method utilizes specific orchid parts, such as Taiwan Patent Application Publication No. 201420107, entitled "Extract of White Phalaenopsis Petals and its Preparation Method and Uses," which discloses an extract of the petals of white Phalaenopsis amabilis (or Phalaenopsis aphrodite) and its preparation method. This extract can be used to promote skin whitening, enhance skin hydration, and prevent and / or delay skin aging. However, the aforementioned Publication No. 201420107 only discloses the relevant technology for extracting white Phalaenopsis petals and its preparation method. Therefore, existing orchid extraction technologies still require further development to utilize other orchid parts to extract extracts with antifungal, skin-care, and antioxidant effects.

[0008] Another existing cosmetic containing orchid extract is the U.S. Patent Application Publication No. 20110002968, entitled "Cosmetic or Dermatological Composition Containing an Orchid Extract, and Cosmetic Care Method Using Said Composition," which discloses a cosmetic composition containing orchid extract and a method for treating skin with the cosmetic composition.

[0009] Another existing cosmetic containing orchid extract is U.S. Patent Application Publication No. 20090258093, entitled "Use ofa brassocattleya marcella koss orchid extract as an active agent to prevent or delay the appearance of signs of cutaneous aging," which discloses an activator that uses orchid extract to prevent or delay skin aging.

[0010] Another existing cosmetic containing orchid extract is the U.S. Patent Application Publication No. 20090238848, entitled "Use ofan Extract of the Orchid Vanda Coerulea as a Skin Hydrating Agent," which discloses a skin moisturizer using Vanda coerulea extract.

[0011] Another existing cosmetic containing orchid extract is the U.S. Patent Application Publication No. 20090232758, entitled "Brassocattleya marcella koss orchid extract and use thereof as skin depigmentation," which discloses a related use of orchid extract for skin depigmentation.

[0012] In fact, the aforementioned U.S. patent applications Nos. 20110002968, 20090258093, 20090238848, and 20090232758 only disclose the use of orchid extracts for various skincare treatments; they do not disclose how orchid seed extracts can achieve antifungal, skincare, and antioxidant effects. Therefore, existing orchid extraction technologies still require further development to utilize other orchid-related parts to extract extracts with skincare and antioxidant effects.

[0013] Another existing cosmetic containing orchid extract is the invention patent "Cosmetics Containing Orchid Embryo Extract" (Taiwan Patent Publication No. I423826), which discloses a cosmetic containing orchid embryo extract. This cosmetic comprises at least one cosmetic ingredient and at least one orchid embryo extract. The orchid embryo extract is added to the cosmetic ingredient to form a cosmetic composition. Furthermore, the orchid embryo extract possesses a highly expressed gene cluster that can produce components beneficial to human skin.

[0014] In fact, the aforementioned Taiwan Patent Application Publication No. I423826 only discloses related technologies for cosmetics containing orchid embryo extract. Therefore, there is still a need to further develop existing orchid embryo extraction technologies to utilize other orchid-related parts to extract extracts with antifungal, skin-care, and antioxidant effects. The aforementioned patent applications and patents are merely for reference and illustration of the current state of technological development and are not intended to limit the present invention.

[0015] In view of this, in order to meet the above-mentioned needs, the present invention provides an orchid extract with antifungal function and a composition thereof, wherein an orchid extract is added to a raw material to form an antifungal composition, and the orchid extract provides an antifungal function, and the orchid extract is a crude orchid extract, thereby expanding the application range of existing orchid extracts. Summary of the Invention

[0016] The main objective of the preferred embodiment of the present invention is to provide an orchid extract and composition thereof with antifungal function, wherein an orchid extract is added to a raw material to form an antifungal composition, and the orchid extract provides an antifungal function, and the orchid extract is a crude orchid extract, so as to achieve the effect of the orchid extract providing antifungal function and providing a natural (non-toxic to humans) antifungal product.

[0017] To achieve the above objectives, a preferred embodiment of the present invention provides an orchid extract comprising an extract derived from a part of an orchid, and the orchid extract contains at least one antifungal component, which has antifungal activity.

[0018] In a preferred embodiment of the present invention, the extract is a crude extract or a purified extract.

[0019] The extract of the preferred embodiment of the present invention contains vicilin protein and other active ingredients.

[0020] The extract of the preferred embodiment of the present invention has a predetermined concentration, which is 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL or 400 μg / mL.

[0021] In a preferred embodiment of the present invention, the orchid part is an orchid seed, an orchid leaf, an orchid pseudobulb, an orchid flower, an orchid flower column, or other flower organs.

[0022] The orchid extract additive composition of a preferred embodiment of the present invention comprises:

[0023] At least one raw material having a first predetermined content; and

[0024] At least one orchid extract having a second predetermined content, wherein the orchid extract is extracted from an orchid part, and the orchid extract is added to the raw material to form an antifungal composition;

[0025] The orchid extract contains at least one antifungal component, which has antifungal activity.

[0026] In a preferred embodiment of the present invention, the extract is a crude extract, and the extract or crude extract is suitable for inhibiting Fusarium, Anthrax, Sclerotium leucosus or other plant fungi.

[0027] The extract of the preferred embodiment of the present invention contains vicilin protein and other active ingredients.

[0028] The extract of the preferred embodiment of the present invention has a predetermined concentration, which is 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL or 400 μg / mL.

[0029] In a preferred embodiment of the present invention, the orchid part is an orchid seed, an orchid leaf, an orchid pseudobulb, an orchid flower, an orchid flower column, or other flower organs.

[0030] Another objective of the preferred embodiment of the present invention is to provide an extract of orchid seeds and an additive product thereof, wherein the extract of orchid seeds contains at least one lipid component, and the lipid component contains at least one fatty acid, and the fatty acid can be used for physiological activities such as anti-inflammatory, antibacterial and antioxidant effects, so as to enhance the economic value of orchid seeds.

[0031] To achieve the above objectives, the orchid seed extract of a preferred embodiment of the present invention comprises an extract extracted from an orchid seed, the extract containing at least one lipid component, the lipid component containing at least one fatty acid, and the fatty acid having applications as physiological activities such as anti-inflammatory, antibacterial and antioxidant effects.

[0032] In a preferred embodiment of the present invention, the orchid seed is selected from the seed of Phalaenopsis orchid.

[0033] In a preferred embodiment of the present invention, the orchid seeds are selected from orchid seeds at different growth stages.

[0034] In a preferred embodiment of the present invention, the fatty acid comprises palmitic acid and linoleic acid.

[0035] In a preferred embodiment of the present invention, the ratio of palmitic acid to linolenic acid is 1:4.

[0036] To achieve the above objectives, the orchid seed extract additive product of a preferred embodiment of the present invention comprises:

[0037] At least one cosmetic ingredient having a first predetermined content; and

[0038] At least one orchid seed extract having a second predetermined content, wherein the orchid seed extract is extracted from an orchid seed, and the orchid seed extract is added to the cosmetic ingredient to form a cosmetic composition.

[0039] The orchid seed extract contains at least one lipid component, and the lipid component contains at least one fatty acid.

[0040] In a preferred embodiment of the present invention, the cosmetic raw material is selected from a skin care product raw material, a makeup product raw material, or a perfume raw material to make a skin care product, a makeup product, or a perfume.

[0041] In a preferred embodiment of the present invention, the cosmetic raw material is selected from a powdered raw material, a paste-like raw material, or a liquid raw material.

[0042] In a preferred embodiment of the present invention, the orchid seed is selected from the seed of Phalaenopsis orchid.

[0043] In a preferred embodiment of the present invention, the orchid seeds are selected from orchid seeds at different growth stages.

[0044] In a preferred embodiment of the present invention, the fatty acid comprises palmitic acid and linoleic acid.

[0045] In a preferred embodiment of the present invention, the ratio of palmitic acid to linolenic acid is 1:4. Attached Figure Description

[0046] Figure 1 This is a schematic flowchart of the orchid seed extraction method according to the first preferred embodiment of the present invention.

[0047] Figure 1A This is a schematic flowchart of the extraction method for orchid pseudo-protozoa according to a second preferred embodiment of the present invention.

[0048] Figure 1BThis is a schematic diagram illustrating the experimental results of using orchid extract with antifungal function at various concentrations to inhibit Fusarium, anthrax, or Sclerotium typhimurium, which is a preferred embodiment of the present invention.

[0049] Figure 2 This is a schematic diagram showing the lipid content in an extract of orchid seeds according to a preferred embodiment of the present invention.

[0050] Figure 3 This is a schematic diagram showing the content of various fatty acid components in the extract of orchid seeds according to a preferred embodiment of the present invention.

[0051] Figure 4 This is a schematic diagram of the process of adding orchid extract to the product according to the first preferred embodiment of the present invention.

[0052] Figure 5 This is a schematic diagram of the process of adding orchid seed extract to the product according to a second preferred embodiment of the present invention.

[0053] Figure Labels

[0054] 1: Raw materials; 1a: Cosmetic raw materials; 2: Orchid extract; 20: Orchid seed extract; 3: Antifungal components; 3a: Cosmetic components; 100: Processing equipment. Detailed Implementation

[0055] To fully understand the present invention, preferred embodiments will be described in detail below with reference to the accompanying drawings, but these are not intended to limit the present invention.

[0056] The orchid extract with antifungal properties, its additive products, and the extraction method thereof, as described in the preferred embodiments of the present invention, are applicable to extracts of various orchids (e.g., Phalaenopsis orchids or other orchids) and their seeds, as well as their additive products and extraction methods, but are not intended to limit the scope of the present invention. Furthermore, the orchid extract of the preferred embodiments of the present invention can be added to various processed products, such as medical products, pharmaceuticals, or cosmetics, but are not intended to limit the scope of the present invention.

[0057] For example, in a preferred embodiment of the present invention, the orchid extract with antifungal function is extracted from a part of an orchid, such as an orchid seed, an orchid leaf, an orchid pseudobulb (or embryo), an orchid flower, an orchid flower column, or other floral organs, but this is not intended to limit the scope of the present invention.

[0058] The orchid seed extract of a preferred embodiment of the present invention is rich in lipids, which possess anti-inflammatory, antibacterial, and antioxidant properties. Furthermore, the orchid seed extract has no particular odor.

[0059] For example, in a preferred embodiment of the present invention, Phalaenopsis orchid seeds are used. Phalaenopsis is a monocotyledonous plant, and academic research has shown that Phalaenopsis orchid seeds do not necessarily contain endosperm tissue. Therefore, the present invention uses histochemical staining to confirm that Phalaenopsis orchid seeds are rich in oily components.

[0060] Figure 1 A schematic flowchart illustrating the orchid seed extraction method of the first preferred embodiment of the present invention is shown below. Please refer to... Figure 1 As shown, the orchid seed extraction method of the first preferred embodiment of the present invention includes step S1: First, a predetermined amount of orchid seeds is provided, and the orchid seeds are crushed to obtain orchid seed fragments. For example, the orchid seeds are selected from Phalaenopsis orchid seeds, and the orchid seeds are selected from orchid seeds at different growth stages. For example, the orchid seed fragments are obtained by properly and thoroughly crushing and stirring using a blender or other device with stirring function.

[0061] Please refer to again Figure 1 As shown, the orchid seed extraction method of the first preferred embodiment of the present invention includes step S2: Next, the orchid seed fragments are added to a solvent (e.g., Hexane) in a container to form a suspension.

[0062] Please refer to again Figure 1 As shown, the orchid seed extraction method of the first preferred embodiment of the present invention includes step S3: Next, the suspension is subjected to appropriate extraction treatment to obtain an orchid seed extract. For example, the orchid seed extract is prepared into an orchid seed extract, and the orchid seed extract contains antifungal components, carbohydrate components, fatty acid components, and other components.

[0063] Please refer to again Figure 1 As shown, the orchid seed extraction method of the first preferred embodiment of the present invention includes step S4: Finally, the orchid seed extract is analyzed for its components using a suitable analytical instrument to obtain an antifungal component or other effective components. For example, the analytical instrument includes a nuclear magnetic resonance spectrometer (NMR), a gas chromatography-mass spectrometry (GC-MS), or a high performance liquid chromatography (HPLC).

[0064] For example, this invention uses nuclear magnetic resonance spectroscopy (NMR) to analyze the components contained in Phalaenopsis orchid seeds, and the results show that Phalaenopsis orchid seeds are rich in triglycerides. Furthermore, analysis of Phalaenopsis orchid seeds at different growth stages shows that the oil content of Phalaenopsis orchid seeds decreases over time, indicating that Phalaenopsis orchid seeds do indeed provide this energy for germination and growth.

[0065] Figure 1A A schematic flowchart illustrating the extraction method of orchid pseudo-spheres according to a second preferred embodiment of the present invention is shown below. Please refer to... Figure 1A As shown, the extraction method of orchid pseudospheres according to the second preferred embodiment of the present invention includes step S1: First, a predetermined amount of orchid pseudospheres is provided, and the pseudospheres are crushed to obtain pseudosphere fragments. For example, the orchid pseudospheres are selected from Phalaenopsis orchids, and the orchid pseudospheres are selected from orchid pseudospheres at different growth stages. For example, a pulverizer or other device with a stirring function (mixer) is used to perform appropriate and sufficient pulverization and stirring to obtain the pseudosphere fragments.

[0066] Please refer to again Figure 1A As shown, the extraction method of orchid pseudospheres in the second preferred embodiment of the present invention includes step S2: Next, the pseudosphere fragments are added to a solvent (e.g., Hexane) in a container to form a suspension.

[0067] Please refer to again Figure 1A As shown, the extraction method of orchid pseudospheres according to the second preferred embodiment of the present invention includes step S3: Next, the suspension is subjected to appropriate extraction treatment to obtain a pseudosphere extract. For example, the pseudosphere extract is prepared into a pseudosphere extract, and the pseudosphere extract contains antifungal components, carbohydrate components, fatty acid components, and other components.

[0068] Please refer to again Figure 1A As shown, the extraction method of orchid pseudomorphs in the second preferred embodiment of the present invention includes step S4: Finally, the components of the pseudomorph extract are analyzed by appropriate analytical instruments to obtain antifungal components or other effective components.

[0069] For example, another preferred embodiment of the present invention describes an extraction method for an antifungal orchid extract comprising: preparing a first extract solution comprising Tris-HCl buffer (pH 8.3, 4.8 g), lithium dodecyl sulfate (2.0 g), glycerol (35 mL), ethylenediaminetetraacetic acid (EDTA) (0.5 M stock, 0.4 mL), and a mixture thereof. The extract solution is then diluted with deionized water (ddH2O) to 100 mL (pH 8.25-8.75) and stored at -20°C.

[0070] For example, another preferred embodiment of the extraction method of the antifungal orchid extract of the present invention includes: preparing a second extract (50 mL) comprising a first extract (12.5 mL), glycerol (0.625 mL), lithium dodecyl sulfate (0.75 g), deionized water (ddH2O, 36.875 mL), and a mixture thereof.

[0071] For example, another preferred embodiment of the present invention describes an extraction method for an antifungal orchid extract comprising: preparing a protease inhibitor comprising 1 mM phenymethylsulfonyl fluoride (PMSF), 100 mM phenymethylsulfonyl fluoride (0.174 g) added to methanol (MeOH, 1 mL), and 1 mM phenymethylsulfonyl fluoride (0.1 mL) added to a second extract (per 10 mL).

[0072] For example, another preferred embodiment of the present invention describes a method for extracting an antifungal orchid extract comprising: adding an orchid part to liquid nitrogen and grinding it into powder in a container (e.g., a mortar and pestle) to obtain an orchid powder. Then, the orchid powder is transferred to another container (e.g., a 30 mL glass tube), and 2 mL of extraction solution is added to every 1 gram of the orchid powder to obtain a crude orchid extract.

[0073] For example, another preferred embodiment of the present invention describes an extraction method for an antifungal orchid extract, which includes centrifuging the crude orchid extract in a centrifuge at a predetermined speed and a predetermined temperature (e.g., 4°C) for a predetermined centrifugation time (e.g., 3 minutes) to obtain a crude orchid extract centrifuged liquid.

[0074] For example, another preferred embodiment of the present invention describes an extraction method for an antifungal orchid extract, which includes collecting the supernatant (containing protein) of the crude extract centrifuged liquid of the orchid, wherein the supernatant contains a protein extract with antifungal function, and preferably collecting the supernatant into a microcentrifuge tube to measure its concentration using the protein quantification method (BCA method).

[0075] Figure 1B This diagram illustrates the experimental results (three experiments) of using orchid extract with antifungal function of a preferred embodiment of the present invention to inhibit Fusarium oxysporum fsp. lycopersici, Colletotrichum capsici, or Sclerotium rolfsii at various concentrations. Please refer to... Figure 1B As shown, for example, in preferred embodiments of the present invention, various concentrations (e.g., 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL, 400 μg / mL) are selected to effectively inhibit Fusarium (e.g.) Figure 1B (as shown in the upper part), anthrax bacteria (such as...) Figure 1B As shown in the middle), white silk fungus (such as...) Figure 1B (as shown at the bottom) or other plant fungi.

[0076] Please refer to again Figure 1B As shown, in a preferred embodiment of the present invention, the extract is a crude extract or a purified extract, which contains vicilin protein and other active ingredients, and has a predetermined concentration of 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL, or 400 μg / mL. Furthermore, in a preferred embodiment of the present invention, the orchid part is an orchid seed, an orchid pseudobulb, an orchid column, or other floral organs.

[0077] Figure 2 A schematic diagram illustrating the lipid content in an orchid seed extract according to a preferred embodiment of the present invention. Please refer to... Figure 2 As shown, triacylglycerol contained in orchid seeds was hydrolyzed and analyzed by gas chromatography-mass spectrometry (GC-MS). Then, by comparing the compound molecular database with different time delays, this invention discovered that the orchid seeds are rich in lipid components, which contain at least one fatty acid, and this fatty acid can be used for physiological activities such as anti-inflammatory, antibacterial, and antioxidant effects.

[0078] Figure 3 A schematic diagram illustrating the content of various fatty acid components in an extract of orchid seeds according to a preferred embodiment of the present invention, compared with... Figure 2 Please refer to Figure 2 and Figure 3 As shown, the fatty acid mainly comprises palmitic acid, linolenic acid, and other fatty acid components. Furthermore, in a preferred embodiment of the present invention, the ratio of palmitic acid to linolenic acid is 1:4.

[0079] Please refer to again Figure 2 and Figure 3 As shown, for example, palmitic acid has antioxidant properties; while linoleic acid, with its structure of two double bonds, is an ω-6 unsaturated fatty acid, thus possessing anti-inflammatory properties. Figure 3 The right half is shown.

[0080] Figure 4 This is a schematic diagram illustrating the process of adding orchid extract to a product according to a first preferred embodiment of the present invention. Please refer to... Figure 4 As shown, in the first preferred embodiment of the present invention, an antifungal composition 3 is prepared by processing a raw material 1 and at least one or more orchid extracts (or orchid extract liquid or powder) 2 in an appropriate proportion in a processing device 100.

[0081] For example, raw material 1 is a medicinal ointment or an oil, which includes petrolatum (white or yellow), lanolin, menthol (oil), borneol, borneol, salicylic acid, wintergreen oil, camphor powder (oil), citronella oil, clove oil, silicone oil, tallow, paraffin oil, linseed oil, oleic acid, turpentine, rosin, eucalyptus oil, or others.

[0082] Figure 5 This is a schematic diagram illustrating the process of adding orchid seed extract to a product according to a second preferred embodiment of the present invention. Please refer to... Figure 5 As shown, compared to the first embodiment, the second preferred embodiment of the present invention prepares a cosmetic composition 3a by processing a cosmetic raw material 1a and at least one or more orchid seed extracts (or orchid seed extract liquid or powder) 20 in an appropriate proportion in the processing equipment 100. For example, the cosmetic raw material 1a is selected from a skin care product raw material, a makeup product raw material, or a perfume raw material to prepare a skin care product, a beauty and skin care product, a makeup product, or a perfume. In addition, the cosmetic raw material 1a is selected from a powdered raw material, a paste-like raw material, or a liquid raw material. The beauty and skin care product includes skin conditioning products (e.g., face masks, creams, or serums).

[0083] In another preferred embodiment of the present invention, at least one or more orchid seed extracts (or orchid seed extract liquid or powder) 20 are added to various processed products, which include a medical product, a pharmaceutical product or a cosmetic product.

[0084] The foregoing preferred embodiments are merely illustrative of the present invention and its technical features. The technology of these embodiments can still be implemented by various substantially equivalent modifications and / or substitutions. Therefore, the scope of the present invention shall be determined by the scope defined in the appended claims.

Claims

1. An orchid extract, characterized in that, The product contains at least one antifungal component, obtained by extracting a part of an orchid (Phalaenopsis orchid) using a second extract solution. The orchid part is an orchid pseudosphere. The second extract solution comprises 12.5 mL of a first extract solution, 0.625 mL of glycerol, 0.75 g of lithium dodecyl sulfate, and 36.875 mL of deionized water. 0.1 mL of 1 mM benzyl sulfonyl fluoride is added to every 10 mL of the second extract solution. The first extract solution comprises 4.8 g of pH 8.3 tris(hydroxymethyl)aminomethane hydrochloride buffer, 35 mL of glycerol, 2.0 g of lithium dodecyl sulfate, and 0.4 mL of 0.5 M ethylenediaminetetraacetic acid. Deionized water is added to a final volume of 100 mL. The pH of the first extract solution is 8.25-8.

75. The antifungal component contains vicilin protein and exhibits inhibitory activity against Fusarium oxysporum fsp. lycopersici and Colletotrichum anthracnose. Capsici and / or Sclerotium rolfsii activity.

2. A composition for adding orchid extract, characterized in that, Include: At least one raw material, having a first predetermined content, wherein the at least one raw material comprises a medicinal paste ingredient; and The orchid extract as described in claim 1 has a second predetermined content.

3. The orchid extract additive composition as described in claim 2, characterized in that, The second predetermined content is 150 μg / mL to 400 μg / mL.

4. The use of an orchid extract in the preparation of a composition for inhibiting fungi, characterized in that, The composition comprises the orchid extract as described in claim 1, wherein the orchid extract is present in a concentration of 150 μg / mL to 400 μg / mL, and the fungus is Fusarium oxysporum fsp. lycopersici, Colletotrichum capsici, and / or Sclerotium rolfsii.

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