一种淋病奈瑟菌耐药位点多重检测方法及试剂盒
By designing dedicated primers and HRM technology for multiple detection of drug resistance sites in Neisseria gonorrhoeae, the problems of time-consuming, labor-intensive, and costly existing detection methods have been solved, achieving rapid, accurate, and low-cost detection of drug resistance sites, which is suitable for monitoring drug resistance in Neisseria gonorrhoeae.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
- Filing Date
- 2019-09-10
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for detecting drug resistance in Neisseria gonorrhoeae are time-consuming, labor-intensive, and costly. In particular, whole-genome sequencing and traditional PCR methods are expensive when detecting drug resistance sites in Neisseria gonorrhoeae, and require professional personnel and expensive fluorescently labeled primers or probes.
A dedicated primer for multiplex detection of drug resistance sites in Neisseria gonorrhoeae was designed. Combined with high-resolution melting curve analysis (HRM) technology, the primers and EvaGreen fluorescent dye enable rapid and accurate detection of drug resistance sites, avoiding the use of expensive probes and complex subsequent analysis steps.
It enables rapid, accurate, and low-cost multiplex detection of drug-resistant sites in Neisseria gonorrhoeae, reducing detection costs, improving detection specificity and sensitivity, avoiding false positive results, and allowing direct analysis during closed-tube procedures, saving time, manpower, and resources.
Smart Images
Figure CN110643722B_ABST