A dual reporter probe for monitoring pre-mrna splicing efficiency and preparation method thereof

Abstract

This invention discloses a dual reporter gene probe for monitoring pre-mRNA splicing efficiency and its preparation method. The probe includes a vector pcDNA3.1, gene fragments A, B, and C. Gene fragments A, B, and C are recombined with a linearized pcDNA3.1 vector in an A-B-C sequence. Fragment A is Fluc, fragment B consists of exon 1, intron, and exon 2, and fragment C is Rluc. The nucleotide sequence of the probe is shown in SEQ ID NO. 5. By analyzing the signal ratio Fluc / Rluc generated by the two luciferases using the reporter gene molecular imaging probe of this invention, the splicing efficiency of in vivo pre-mRNA can be analyzed in real time and non-invasively, providing an effective screening tool for drug research regulating pre-mRNA splicing.

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