适用于DNA编码化合物测序文库测序的引物
By designing amplification primers suitable for sequencing DNA-encoded compounds and constructing sequencing libraries using one-step PCR amplification, the problems of low sequencing quality and complex operation in existing technologies are solved, achieving efficient and accurate screening of DNA-encoded compounds.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HITGEN INC
- Filing Date
- 2019-09-29
- Publication Date
- 2026-07-17
AI Technical Summary
Existing DNA-encoded compound sequencing methods suffer from low sequencing quality, numerous operational steps, and the introduction of amplification bias, which affect the authenticity and efficiency of sample screening.
A sequencing library was directly constructed using a one-step PCR amplification with a suitable amplification primer for sequencing DNA-encoded compounds. The primers were designed so that the adapter sequence was linked to the DNA-encoded compound amplification primer sequence, avoiding the formation of complementary sequences and hairpin structures, and optimizing the base content distribution.
It achieves high data utilization and improved sequencing quality, simplifies operation steps, reduces costs, and improves the authenticity of DNA molecules and sequencing accuracy of screened samples.
Smart Images
Figure CN110964794B_ABST