A method and application for simultaneously improving the stability of hydrophobic and water-soluble active ingredients by using cage plant ferritin

Abstract

The present application relates to a kind of method for simultaneously improving the stability of hydrophobic and water-soluble active ingredients using cage-like plant ferritin, the steps are as follows: dissolving water-soluble active ingredients in deionized water to prepare mother liquor, dissolving hydrophobic active ingredients in ethanol to prepare mother liquor; adjust pH, add water-soluble active ingredients to denatured ferritin solution, stir, incubate; adjust pH, mix evenly; adjust temperature to induce channel expansion of ferritin, add hydrophobic active ingredient mother liquor, induce hydrophobic active ingredients to pass through the channel into the internal cavity of ferritin; centrifugation, collect supernatant, obtain ferritin-water-soluble active ingredient-hydrophobic active ingredient complex.The complex obtained by the method has good water solubility and high embedding rate, and effectively improves the thermal stability of water-soluble active ingredients, improves the solubility and thermal stability of hydrophobic active ingredients, improves the stability of two active molecules in simulated gastrointestinal fluid, and improves the application range of ferritin.

Images