A multi-adjustable cell migration culture device

Abstract

The application relates to the field of cell culture, and particularly relates to a multi-adjustable cell moving culture device, which comprises a horizontally arranged supporting installation plate, a vertically arranged lifting installation cylinder arranged at the middle position of the supporting installation plate, a vertically arranged lifting installation column matched with the lifting installation cylinder, lifting installation cylinder outer sides between the lifting installation column and the limiting installation plate are vertically arranged with lifting damping springs, lifting direction damping and swing suppression damping are adopted, the stability of the device during movement is remarkably improved, the cell culture conditions can be greatly met through pressure compensation adjustment, humidity adjustment and temperature adjustment, and bacteria and viruses in the air in the device are killed through ultraviolet disinfection combined with air compensation, so that the quality of cell culture is ensured.

Description

Technical Field

[0001] This invention relates to the field of cell culture, specifically a multi-regulatory cell movement culture device. Background Technology

[0002] In the fields of biology and medicine, cell culture is a fundamental experimental technique used to simulate the in vivo environment (sterility, suitable temperature, pH, and specific nutritional conditions, etc.) in vitro, enabling cells to survive, grow, reproduce, and maintain their main structures and functions. It is used to study cell signal transduction, cellular synthesis and metabolism, and cell growth and proliferation. This invention provides a cell culture device under a specific gas, used to test fundamental biological experimental parameters such as cell growth, reproduction, and metabolism under a specific gas environment.

[0003] Chinese patent (publication number CN110628622A) discloses a mobile portable gas-generating cell culture device. Although it is also a completely closed gas reaction generating structure that can carry out portable cell culture, it has defects in terms of stability during movement and multi-parameter adjustment of cell culture. In addition, it lacks sterilization and disinfection environment support, resulting in poor cell culture quality. Summary of the Invention

[0004] The purpose of this invention is to provide a multi-modality adjustable cell movement culture device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A multi-adjustable cell mobile culture device includes a horizontally arranged support mounting plate, a vertically positioned lifting mounting cylinder at the center of the support mounting plate, and a vertically positioned lifting mounting column cooperating with the lifting mounting cylinder. Both ends of the lifting mounting column extend beyond the lifting mounting cylinder. A horizontally positioned swing mounting cover is located at the lower end of the lifting mounting column, and a horizontally positioned limiting mounting plate is located at the upper end of the lifting mounting column. A vertically positioned lifting shock-absorbing spring is located on the outer side of the lifting mounting column between the limiting mounting plate and the lifting mounting cylinder. A rotating mounting disk is positioned at the center of the swing mounting cover via a support shaft. A support mounting cover is horizontally positioned at the lower end of the rotating mounting disk via a fixed column. A liquid storage tank is horizontally positioned at the center of the bottom of the support mounting cover. Gas storage cylinders are horizontally positioned at the bottom of the support mounting covers on both sides of the liquid storage tank. Support mounting frames are symmetrically positioned at the upper ends of the gas storage cylinders, and each support mounting frame has a longitudinally positioned limiting guide groove at its upper end. The upper end of each support mounting frame is vertically equipped with a mesh mounting cylinder. The lower end of each mesh mounting cylinder is equipped with a limit guide post in conjunction with the limit guide groove. Inside the mesh mounting cylinder, several perforated support plates are horizontally arranged at equal intervals. Inside each support mounting frame, an electric auxiliary heating mesh is horizontally arranged. A guide pipe is vertically installed through the air storage cylinder directly below the support mounting frame. A pressure-changing cylinder is vertically installed at the upper end of the liquid storage tank. A pressure-changing piston plate is horizontally arranged inside the pressure-changing cylinder. A hydraulic lifting column is vertically installed at the bottom center of the pressure-changing cylinder. The upper end of the hydraulic lifting column is connected to the pressure-changing piston plate. A slow-flow chamber is set inside the pressure-changing piston plate. Several telescopic guide tubes are arranged at equal angles at the lower end of the slow-flow chamber. The telescopic guide tubes extend downward into the liquid storage tank. A humidifier is vertically installed at the upper center of the pressure-changing piston plate. The lower end of the humidifier extends into the bottom of the slow-flow chamber. A rotating sealing plate is installed at the front end of the support mounting cover via a hinge.

[0006] As a further embodiment of the present invention: lifting guide columns are symmetrically arranged on the outer side of the lifting mounting column, all of which are vertically arranged, and the inner wall of the lifting mounting cylinder is provided with lifting guide grooves in accordance with the lifting guide columns.

[0007] As a further embodiment of the present invention: both ends of the rotating mounting plate are symmetrically provided with connecting mounting posts, and the inside of the swing mounting cover is symmetrically provided with limiting mounting posts, and the limiting mounting posts and the connecting mounting posts are connected one-to-one by a return spring.

[0008] As a further aspect of the present invention: three sets of support mounting rings are vertically arranged at the upper end of the porous support plate. The support mounting rings are arranged in concentric circles, and the height of the support mounting rings increases from the inside to the outside.

[0009] As a further aspect of the present invention: an arc-shaped guide tube is provided directly above each porous support plate, and the outer side of the arc-shaped guide tube is fixed inside the mesh mounting tube.

[0010] As a further aspect of the present invention: each of the guide pipes is equipped with a solenoid valve, and each of the gas storage cylinders is equipped with a horizontally mounted ultraviolet disinfection lamp at the bottom.

[0011] As a further embodiment of the present invention: the internal connecting telescopic conduit of the liquid storage tank is provided with a liquid extraction conduit, the lower end of the liquid extraction conduit is provided with a perforated guide plate, and the upper left end of the liquid storage tank is provided with a water supply pipe.

[0012] Compared with the prior art, the beneficial effects of the present invention are: by lifting and guiding shock absorption and swing suppression shock absorption, the stability of the device during movement is significantly improved. At the same time, by adjusting the pressure, humidity and temperature, the conditions for cell culture can be greatly met. Furthermore, ultraviolet disinfection combined with air compensation kills bacteria and viruses in the air inside the device, ensuring the quality of cell culture. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of a multi-regulatory cell movement culture device.

[0014] Figure 2 for Figure 1 An enlarged schematic diagram of point a in the middle.

[0015] Figure 3 This is a three-dimensional schematic diagram of a porous support plate in a multi-adjustable cell movement culture device.

[0016] 1-Liquid storage tank, 2-Gas storage cylinder, 3-Mesh mounting cylinder, 4-Rotating sealing plate, 5-Support mounting cover, 6-Swing mounting cover, 7-Limit mounting post, 8-Rotating mounting plate, 9-Support shaft, 10-Connecting mounting post, 11-Reset spring, 12-Support mounting plate, 13-Lifting guide groove, 14-Lifting mounting cylinder, 15-Lifting shock absorber spring, 16-Limit mounting plate, 17-Lifting guide post, 18-Lifting mounting post, 19-Support 20-Mounting ring, 21-Porous support plate, 22-Arc-surface guide tube, 23-Limiting guide column, 24-Limiting guide groove, 25-Support mounting frame, 26-Electric auxiliary heating network, 27-Guide pipe, 28-Solenoid valve, 29-Ultraviolet disinfection lamp, 30-Water supply pipe, 31-Porous guide plate, 32-Liquid extraction guide pipe, 33-Hydraulic lifting column, 34-Telescopic guide pipe, 35-Humidifier, 36-Pressure-changing piston plate, 37-Pressure-changing cylinder. Detailed Implementation

[0017] Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0018] The following disclosure provides numerous different embodiments or examples for implementing various structures of the invention. To simplify the disclosure, specific examples of components and arrangements are described below. These are merely examples and are not intended to limit the scope of the invention. Furthermore, reference numerals and / or letters may be repeated in different examples. Such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed.

[0019] Example 1 Please see Figures 1-3In this embodiment of the invention, a multi-adjustable cell movement culture device includes a horizontally arranged support mounting plate 12. A lifting mounting cylinder 14 is vertically arranged in the middle of the support mounting plate 12. A lifting mounting column 18 is vertically arranged in conjunction with the lifting mounting cylinder 14. Both ends of the lifting mounting column 18 extend out of the lifting mounting cylinder 14. A swing mounting cover 6 is horizontally arranged at the lower end of the lifting mounting column 18. A limiting mounting plate 16 is horizontally arranged at the upper end of the lifting mounting column 18. A lifting shock-absorbing spring 15 is vertically arranged on the outer side of the lifting mounting column 18 between the limiting mounting plate 16 and the lifting mounting cylinder 14. Lifting guide columns 17 are symmetrically arranged on the outer side of the lifting mounting column 18. The lifting guide columns 17 are all vertically arranged, and the inner wall of the lifting mounting cylinder 14 is equipped with... Each of the lifting guide columns 17 is vertically equipped with a lifting guide groove 13. A rotating mounting plate 8 is mounted on the middle of the swing mounting cover 6 via a supporting shaft 9. A supporting mounting cover 5 is horizontally mounted on the lower end of the rotating mounting plate 8 via a fixed column. Connecting mounting columns 10 are symmetrically arranged at both ends of the rotating mounting plate 8. Limiting mounting columns 7 are symmetrically arranged inside the swing mounting cover 6. The limiting mounting columns 7 and connecting mounting columns 10 are connected one-to-one by return springs 11. A liquid storage tank 1 is horizontally arranged at the middle of the bottom of the supporting mounting cover 5. Air storage cylinders 2 are horizontally arranged at the bottom of the supporting mounting covers 5 on both sides of the liquid storage tank 1. Support mounting frames 24 are symmetrically arranged at the upper ends of the air storage cylinders 2. Limiting guide grooves 23 are longitudinally arranged at the upper ends of the support mounting frames 24. Each support mounting frame 24 has a vertically installed mesh mounting cylinder 3 at its upper end. The lower end of each mesh mounting cylinder 3, in conjunction with the limiting guide groove 23, is equipped with a limiting guide post 22. Inside the mesh mounting cylinder 3, several perforated support plates 20 are horizontally arranged at equal intervals. Each perforated support plate 20 has three sets of vertically installed support mounting rings 19 at its upper end. The support mounting rings 19 are concentric circles, with their height increasing from the inside out. Each perforated support plate 20 has an arc-shaped guide tube 21 directly above it, with the outer side of the arc-shaped guide tube 21 fixed inside the mesh mounting cylinder 3. The support mounting frame 24 has a horizontally installed electric auxiliary heating mesh 25. A guide pipe 26 is vertically installed through the gas storage tank 2 directly below the support mounting frame 24. Each of the components 6 is equipped with a solenoid valve 27. Each of the gas storage cylinders 2 has a horizontally mounted ultraviolet disinfection lamp 28 at its bottom. Each of the liquid storage tanks 1 has a vertically mounted pressure-changing cylinder 37 at its upper end. Inside the pressure-changing cylinder 37, a pressure-changing piston plate 36 is horizontally mounted. A hydraulic lifting column 32 is vertically mounted at the bottom center of the pressure-changing cylinder 37. The upper end of the hydraulic lifting column 32 is connected to the pressure-changing piston plate 36. Inside the pressure-changing piston plate 36, a slow-flow chamber 34 is provided. At equal angles, several telescopic guide tubes 33 are mounted on the lower end of the slow-flow chamber 34. The telescopic guide tubes 33 extend downwards into the liquid storage tank 1. Each of the telescopic guide tubes 33 is connected to a liquid extraction tube 31 inside the liquid storage tank 1. Each liquid extraction tube 31 has a perforated guide plate 30 at its lower end. A water supply pipe 29 is located on the upper left side of the liquid storage tank 1.A humidifier 35 is vertically mounted at the upper center of the transformer piston plate 36. The lower end of the humidifier 35 extends into the bottom of the slow-flow chamber 34. A rotating sealing plate 4 is hinged to the front end of the support mounting cover 5.

[0020] By placing the cell culture dish on the support mounting ring 19, and then installing the mesh mounting cylinder 3 on the support mounting frame 24 through the limiting guide column 22 and the limiting guide groove 23, the cell culture dish is placed by rotating the sealing plate 4. The device is then fixed on the mobile transfer equipment with the fixed mounting plate. When the device is in the moving state, the vertical shock absorption is achieved by the cooperation of the lifting mounting column 18 and the lifting mounting cylinder 14, and by the lifting shock absorption spring 15. The swing is suppressed by the return spring 11, which significantly reduces the swing amplitude of the support mounting cover 5, making the device more stable during the movement. The internal pressure is adjusted by lifting the pressure-changing piston plate 36 through the hydraulic lifting column 32. The pressure is restored by the solenoid valve 27. The internal temperature range is adjusted by the electric auxiliary heating network 25. The liquid in the storage tank 1 is drawn by the humidifier 35 and enters the slow flow chamber 34 through the porous guide plate 30, the liquid drawing pipe 31, and the telescopic pipe 33, thereby adjusting the internal humidity.

[0021] Example 2 Based on Example 1, multiple sets of support mounting rings 19 can adapt to cell culture dishes of different sizes, making the fixation of cell culture dishes simple and stable. The air entering the air storage box is sterilized under the action of ultraviolet disinfection lamp 28, ensuring the safety of the culture environment and improving the quality of cell culture.

[0022] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0023] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A multi-adjustable cell movement culture device, comprising a horizontally arranged support mounting plate (12), a vertically arranged lifting mounting cylinder (14) at the middle position of the support mounting plate (12), a vertically arranged lifting mounting column (18) cooperating with the lifting mounting cylinder (14), both ends of the lifting mounting column (18) extending out of the lifting mounting cylinder (14), symmetrically arranged lifting guide columns (17) on the outer side of the lifting mounting column (18), the lifting guide columns (17) being vertically arranged, and the inner wall of the lifting mounting cylinder (14) cooperating with the lifting guide columns (17) being vertically arranged with lifting guide grooves (13), a horizontally arranged swing mounting cover (6) at the lower end of the lifting mounting column (18), and a horizontally arranged limit mounting plate (16) at the upper end of the lifting mounting column (18), characterized in that, A lifting damping spring (15) is vertically installed on the outside of the lifting mounting column (18) between the limiting mounting plate (16) and the lifting mounting cylinder (14). A rotating mounting plate (8) is installed in the middle of the swing mounting cover (6) via a support shaft (9). Connecting mounting columns (10) are symmetrically installed at both ends of the rotating mounting plate (8). Limiting mounting columns (7) are symmetrically installed inside the swing mounting cover (6). The limiting mounting columns (7) and the connecting mounting columns (10) are connected one-to-one by a return spring (11). A support mounting cover (5) is horizontally installed at the lower end of the rotating mounting plate (8) via a fixing column. A liquid storage tank (1) is horizontally installed at the middle of the bottom of the support mounting cover (5). Support mounting covers are installed on both sides of the liquid storage tank (1). (5) A gas storage cylinder (2) is horizontally arranged at the bottom. A support mounting frame (24) is symmetrically arranged at the upper end of the gas storage cylinder (2). A limit guide groove (23) is longitudinally arranged at the upper end of the support mounting frame (24). A mesh mounting cylinder (3) is vertically arranged at the upper end of the support mounting frame (24). A limit guide post (22) is arranged at the lower end of the mesh mounting cylinder (3) in conjunction with the limit guide groove (23). Several perforated support plates (20) are horizontally arranged vertically at equal intervals inside the mesh mounting cylinder (3). Three sets of support mounting rings (19) are vertically arranged at the upper end of each perforated support plate (20). The support mounting rings (19) are arranged in concentric circles, and the height of the support mounting rings (19) increases from the inside to the outside. Each perforated support plate (20) has a height of 10 mm. Above each of the gas storage tanks (1) is an arc-shaped guide tube (21), and the outer side of the arc-shaped guide tube (21) is fixed inside the mesh mounting tube (3). The inside of the support mounting frame (24) is horizontally equipped with an electric auxiliary heating net (25). The gas storage tank (2) directly below the support mounting frame (24) is vertically equipped with a guide tube (26). The inside of the guide tube (26) is equipped with a solenoid valve (27). The bottom of the gas storage tank (2) is horizontally equipped with an ultraviolet disinfection lamp (28). The upper end of the liquid storage tank (1) is vertically equipped with a pressure-changing cylinder (37). The inside of the pressure-changing cylinder (37) is horizontally equipped with a pressure-changing piston plate (36). The bottom middle position of the pressure-changing cylinder (37) is vertically equipped with a hydraulic lifting column (32). The upper end of the container is connected to the pressure-changing piston plate (36). The pressure-changing piston plate (36) is provided with a slow-flow chamber (34). Several telescopic conduits (33) are provided at equal angles at the lower end of the slow-flow chamber (34). The telescopic conduits (33) extend downward into the liquid storage tank (1). A humidifier (35) is vertically provided at the middle position of the upper end of the pressure-changing piston plate (36). The lower end of the humidifier (35) extends into the bottom of the slow-flow chamber (34). A rotating sealing plate (4) is provided at the front end of the support mounting cover (5) through a hinge. The telescopic conduits (33) connected inside the liquid storage tank (1) are all provided with a liquid extraction conduit (31). The lower end of the liquid extraction conduit (31) is provided with a multi-hole guide plate (30). A water supply pipe (29) is provided at the upper left end of the liquid storage tank (1).

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