Multifunctional diaphragm and biological microwell plate microscopic imaging light path device

Abstract

The application provides a multifunctional diaphragm and a biological microwell plate microscopic imaging light path device, the multifunctional diaphragm comprises a diaphragm plate provided with at least four through holes; a functional piece is arranged in the through hole, the functional piece comprises any one or a combination of at least two of a homogenizing sheet, a prism or a filter; the biological microwell plate whole-well microscopic imaging light path device comprises a light source assembly, a diaphragm assembly and an imaging lens assembly arranged in sequence, and the diaphragm assembly is the multifunctional light. The multifunctional diaphragm adjusts the incident angle, light flux, light intensity or wavelength of light emitted by the light source assembly into the imaging lens assembly through the functional piece; so that the biological microwell plate microscopic imaging light path device can accurately measure different detection samples.

Description

Technical Field

[0001] This invention belongs to the field of optical technology and relates to a microscope optical path device, particularly a multifunctional aperture and biological microplate microscopic imaging optical path device. Background Technology

[0002] Due to surface tension and the water-repellent properties of liquids in contact with different objects, liquids often exhibit concave liquid surfaces in containers containing liquids. This causes light to scatter at the liquid's edge when a beam of light shines perpendicularly on the liquid, resulting in the light appearing darker at the liquid's edge.

[0003] Similar situations inevitably arise in microscopic imaging. Due to the concave surface of the liquid, edge effects occur during microscopic imaging, causing the edge light to darken and the sample to be unclear.

[0004] CN 108707551A discloses a cell observation device and method. The cell observation device includes: a cell culture plate with multiple wells for holding culture medium; a liquid addition mechanism for adding culture medium to the desired wells; and a microscopic imaging mechanism for observing cells in the wells after the liquid addition mechanism has added culture medium. The microscopic imaging mechanism includes a parallel light source, a microscope objective, a mirror, and a camera arranged sequentially along the display light path, with the cell culture plate located between the parallel light source and the microscope objective. By adding culture medium to the wells for cell observation, the cell observation device changes the liquid surface of the culture medium from a concave liquid surface to a flat liquid surface, eliminating the phenomenon of black rings at the well edges and improving the observation effect.

[0005] However, the aforementioned observation device and corresponding observation method require the addition of an additional object to be observed, which makes the observation results susceptible to various influences and hinders the improvement of detection accuracy. Therefore, it is necessary to improve the detection accuracy by modifying the optical path. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a multifunctional aperture and a biological microplate microscopic imaging optical path device. The multifunctional aperture, used in the biological microplate microscopic imaging optical path device, enables the adjustment of the incident angle, luminous flux, luminous intensity, or wavelength of the light emitted by the light source component into the imaging lens component, thereby enabling the biological microplate microscopic imaging optical path device to accurately measure different test samples with different concave meniscus conditions.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a multifunctional aperture, the multifunctional aperture comprising an aperture plate having at least four through holes;

[0009] The through hole is provided with a functional component, which includes any one or a combination of at least two of the following: a light-diffusing plate, a prism, or a filter.

[0010] The multifunctional aperture of this invention has at least four through holes, for example, four, five, six, seven, eight, nine, or ten, but is not limited to the listed values; other unlisted values ​​within the range are also applicable. Each of the at least four through holes is provided with any one or a combination of at least two of a light-diffusing plate, a prism, or a filter.

[0011] The light homogenizer makes the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light. This invention achieves the "multi-functionality" of the aperture by setting different functional components in different through-holes of the aperture plate.

[0012] The functional component provided in the through hole of the present invention includes any one or at least two combinations of a light-diffusing plate, a prism, or a filter. This means: any one of a light-diffusing plate, a prism, or a filter is provided in the through hole; or, a prism and a light-diffusing plate are provided in the same through hole; or, a prism and a filter are provided in the same through hole; or, a light-diffusing plate and a filter are provided in the same through hole; or, a light-diffusing plate, a prism, or a filter are provided in the same through hole.

[0013] More preferably, the prism is a prism with a light equalization function and / or a prism with a light filtering function.

[0014] This invention does not specifically limit the placement of the light-diffusing plate, prism, and filter in the through hole. Those skilled in the art can reasonably set their positions in the through hole according to their functions.

[0015] Preferably, the shape of the through hole includes any one of a circle, an ellipse, a semicircle, or a combination of shapes; the combination of shapes includes a combination of a semicircle and a square or a combination of an ellipse and a square, and the square includes a rectangle or a square; the shapes of the through holes on the aperture plate are the same and / or different, that is, the shape of each of the at least 4 through holes is any one of a circle, an ellipse, a semicircle, or a combination of shapes.

[0016] Preferably, the apertures of the at least four through holes are different; through holes with different apertures can adjust the light flux entering the imaging lens assembly. By making the apertures different, the present invention can adjust the light flux while adjusting the optical path.

[0017] Preferably, the at least four through holes are evenly distributed with the center of the aperture plate as the center.

[0018] Since the shape of the through holes described in this invention includes any one of the following: circular, elliptical, semi-circular, or combined patterns, and the diameters of the through holes are different, the at least four through holes uniformly distributed with the center of the aperture plate as the center of the circle in this invention means that the center of the circular, elliptical, semi-circular, or combined pattern of the through holes is uniformly distributed with the center of the aperture plate as the center of the circle.

[0019] In a second aspect, the present invention provides a biological microplate microscopic imaging optical path device, the biological microplate microscopic imaging optical path device comprising a light source assembly, an aperture assembly and an imaging lens assembly arranged sequentially; the aperture assembly is the multifunctional aperture described in the first aspect.

[0020] The central axis of the light source assembly coincides with the central axis of the multifunctional aperture.

[0021] When measuring a sample with a concave liquid surface using the microscopic imaging optical path device for biological microplates provided by this invention, one of the at least four through holes of the multifunctional aperture is positioned between the light source assembly and the imaging lens assembly. The relevant area is observed through the shape of the through hole and its functional components. After the observation is completed, the other through holes in the multifunctional aperture are switched, and the relevant area of ​​the concave liquid surface is observed using the switched through holes. The above steps are repeated until the entire area of ​​the concave liquid surface is observed. Then, conventional image processing software is used to integrate the observed images to obtain a clear image of the sample with a concave liquid surface.

[0022] The through holes of the multifunctional aperture correspond to the liquid surface area of ​​the sample to be tested. That is, the liquid surface area to be tested is divided into n regions by n through holes. The optical fiber of the imaging lens assembly is introduced through each through hole to illuminate the corresponding region, where n is an integer ≥ 4.

[0023] Because the through-hole of the multifunctional aperture is provided with functional components, the functional components include any one or a combination of at least two of the following: a light homogenizer, a prism, or a filter; wherein the light homogenizer can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; and the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0024] Preferably, the light source assembly includes a light source base, a sealing ring, an LED light source, a fixing component, and a heat sink; the sealing ring and the fixing component are used to fix the LED light source to the light source base; the heat sink is installed on the end face of the light source base and contacts the LED light source for heat dissipation of the LED light source.

[0025] The light source holder described in this invention is a light source holder with a through hole. The LED light source is disposed in the through hole, and the central axis of the LED light source coincides with the central axis of the through hole. The sealing ring and the fixing member are used to securely install the LED light source in the light source holder. The fixing member is a conventional annular fixing member in the art, and this invention does not specifically limit it.

[0026] The heat sink is used to dissipate heat from the LED light source, and includes air-cooled and / or water-cooled heat sinks. This invention does not specifically limit the structure of the air-cooled or water-cooled heat sinks; those skilled in the art can make reasonable settings as needed.

[0027] Preferably, the central axes of the light source holder, sealing ring, LED light source, fixing component, and heat sink are coincident.

[0028] Preferably, the imaging lens assembly includes a lens barrel and a lens group fixed inside the lens barrel by a fixing member.

[0029] Preferably, the principal axes of the lenses in the lens group coincide.

[0030] Preferably, along the direction of light illumination, the lens group includes a first lens, a second lens, a third lens, a fourth lens, and a fifth lens arranged sequentially.

[0031] Preferably, the first lens is a meniscus lens; the second lens is a plano-convex lens; the third lens is a plano-convex lens; the fourth lens is a biconvex lens; and the fifth lens is a meniscus lens.

[0032] The optical fiber passing through the aperture sequentially enters the concave surface of the first lens, the flat surface of the second lens, the flat surface of the third lens, the convex surface of the fourth lens, and the convex surface of the fifth lens.

[0033] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0034] (1) This invention makes the structure of the aperture plate compact by opening at least 4 through holes and setting different functional components in the through holes, and the light homogenizer can make the light from the light source more uniform, thereby improving the light path; the prism can make the angle of the light from the light source change appropriately; the filter can intercept the required monochromatic light for fluorescence experiments other than white light; this invention achieves the "multi-functionality" of the aperture by setting different functional components in different through holes of the aperture plate.

[0035] (2) The present invention combines a light source assembly, an aperture assembly, and an imaging lens assembly into a biological microplate microscopic imaging optical path device. When the biological microplate microscopic imaging optical path device is used to measure a sample with a concave liquid surface, the through holes of the multifunctional aperture correspond to the liquid surface area of ​​the sample to be measured. That is, n through holes divide the liquid surface area to be measured into n regions. The optical fiber of the imaging lens assembly is introduced through each through hole to illuminate the corresponding region, where n is an integer ≥ 4. The biological microplate microscopic imaging optical path device, through the setting of the multifunctional aperture, enables those skilled in the art to adjust the optical path according to the situation of each region of the sample to be measured, so that the final synthesized image of each region is clear and complete. Attached Figure Description

[0036] Figure 1 This is a schematic diagram of the structure of the biological microplate microscopic imaging optical path device of the present invention;

[0037] Figure 2 This is a schematic diagram of the structure of the light source assembly described in this invention;

[0038] Figure 3 This is a schematic diagram of the lens group described in this invention;

[0039] Figure 4 This is a schematic diagram of the structure of the multifunctional aperture in Embodiment 1 of the present invention;

[0040] Figure 5 This is a schematic diagram of the structure of the multifunctional aperture in Embodiment 2 of the present invention;

[0041] Figure 6 This is a schematic diagram of the structure of the multifunctional aperture in Embodiment 3 of the present invention;

[0042] Figure 7 This is a schematic diagram of the structure of the multifunctional aperture in Embodiment 4 of the present invention;

[0043] Figure 8 This is a partition diagram of the liquid surface to be tested in Application Example 1 of the present invention;

[0044] Figure 9 This is a schematic diagram of the structure of the multifunctional aperture in Embodiment 5 of the present invention;

[0045] Figure 10 This is a partition diagram of the liquid surface to be tested in Application Example 4 of the present invention;

[0046] Figure 11 This is the final synthesized image obtained in Application Example 1 of the present invention;

[0047] Figure 12 This is the final synthesized image obtained in Comparative Application Example 1 of the present invention.

[0048] The components are as follows: 1. Fixing component; 2. Lens barrel; 3. Lens group; 3-1. First lens; 3-2. Second lens; 3-3. Third lens; 3-4. Fourth lens; 3-5. Fifth lens; 4. Multifunctional aperture; 4-1. First through hole; 4-2. Second through hole; 4-3. Third through hole; 4-4. Fourth through hole; 4-5. Fifth through hole; 4-6. Sixth through hole; 4-7. Seventh through hole; 4-8. Eighth through hole; 5. Light source holder; 6. Sealing ring; 7. LED light source; 8. Heat sink. Detailed Implementation

[0049] It should be understood that in the description of this invention, the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0050] It should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "set," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0051] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0052] Example 1

[0053] This embodiment provides a microscopic imaging optical path device for biological microplates. A schematic diagram of the structure of the microscopic imaging optical path device for biological microplates is shown below. Figure 1 As shown, it includes three components arranged in sequence: a light source assembly, an aperture assembly, and an imaging lens group; the central axis of the light source assembly, the central axis of the three imaging lens groups, and the central axis of the multifunctional aperture 4 coincide.

[0054] A schematic diagram of the structure of the light source assembly is shown below. Figure 2As shown, it includes a light source base 5, a sealing ring 6, an LED light source 7, a fixing component 1, and a heat sink 8. The central axes of the light source base 5, the sealing ring 6, the LED light source 7, the fixing component 1, and the heat sink 8 are coincident. The sealing ring 6 and the fixing component 1 are used to fix the LED light source 7 to the light source base 5. The heat sink 8 is installed on the end face of the light source base 5 and contacts the LED light source 7 for heat dissipation of the LED light source 7.

[0055] The imaging lens assembly includes a lens barrel 2 and a lens assembly 3 fixed inside the lens barrel 2 by a fixing member 1. A schematic diagram of the structure of the lens assembly 3 is shown below. Figure 3 As shown, the principal axes of all lenses in lens group 3 coincide. Along the direction of light illumination, lens group 3 includes a first lens 3-1, a second lens 3-2, a third lens 3-3, a fourth lens 3-4, and a fifth lens 3-5 arranged sequentially. Specifically, the first lens 3-1 is a meniscus lens; the second lens 3-2 is a plano-convex lens; the third lens 3-3 is a plano-convex lens; the fourth lens 3-4 is a biconvex lens; and the fifth lens 3-5 is a meniscus lens. Light rays passing through the aperture sequentially enter the concave surface of the first lens 3-1, the plane of the second lens 3-2, the plane of the third lens 3-3, the convex surface of the fourth lens 3-4, and the convex surface of the fifth lens 3-5.

[0056] The aperture assembly is as follows: Figure 4 The multifunctional aperture 4 shown includes an aperture plate with four through holes, which are evenly distributed around the center of the aperture plate. Specifically, the four through holes are a first through hole 4-1, a second through hole 4-2, a third through hole 4-3, and a fourth through hole 4-4. The first through hole 4-1 and the fourth through hole 4-4 are circular through holes, while the second through hole 4-2 and the third through hole 4-3 are combined graphic through holes, which are a combination of a semi-circle and a square. A prism is provided in the first through hole 4-1, a light diffuser is provided in the second through hole 4-2, a filter is provided in the third through hole 4-3, and a combination of a prism and a filter is provided in the fourth through hole 4-4.

[0057] The combination of prism and filter includes not only a combination of a single prism and a single filter, but also a prism coated with a filter film.

[0058] When using the biological microplate microscopic imaging optical path device provided in this embodiment to measure the liquid to be tested with a concave liquid surface, a clear image can be obtained.

[0059] Example 2

[0060] This embodiment provides a microscopic imaging optical path device for biological microplates. A schematic diagram of the structure of the microscopic imaging optical path device for biological microplates is shown below. Figure 1As shown, it includes three components arranged in sequence: a light source assembly, an aperture assembly, and an imaging lens group; the central axis of the light source assembly, the central axis of the three imaging lens groups, and the central axis of the multifunctional aperture 4 coincide.

[0061] A schematic diagram of the structure of the light source assembly is shown below. Figure 2 As shown, it includes a light source base 5, a sealing ring 6, an LED light source 7, a fixing component 1, and a heat sink 8. The central axes of the light source base 5, the sealing ring 6, the LED light source 7, the fixing component 1, and the heat sink 8 are coincident. The sealing ring 6 and the fixing component 1 are used to fix the LED light source 7 to the light source base 5. The heat sink 8 is installed on the end face of the light source base 5 and contacts the LED light source 7 for heat dissipation of the LED light source 7.

[0062] The imaging lens assembly includes a lens barrel 2 and a lens assembly 3 fixed inside the lens barrel 2 by a fixing member 1. A schematic diagram of the structure of the lens assembly 3 is shown below. Figure 3 As shown, the principal axes of all lenses in lens group 3 coincide. Along the direction of light illumination, lens group 3 includes a first lens 3-1, a second lens 3-2, a third lens 3-3, a fourth lens 3-4, and a fifth lens 3-5 arranged sequentially. Specifically, the first lens 3-1 is a meniscus lens; the second lens 3-2 is a plano-convex lens; the third lens 3-3 is a plano-convex lens; the fourth lens 3-4 is a biconvex lens; and the fifth lens 3-5 is a meniscus lens. Light rays passing through the aperture sequentially enter the concave surface of the first lens 3-1, the plane of the second lens 3-2, the plane of the third lens 3-3, the convex surface of the fourth lens 3-4, and the convex surface of the fifth lens 3-5.

[0063] The aperture assembly is as follows: Figure 5 The multifunctional aperture 4 shown includes an aperture plate with four through holes, which are evenly distributed around the center of the aperture plate. Specifically, the four through holes are a first through hole 4-1, a second through hole 4-2, a third through hole 4-3, and a fourth through hole 4-4. The first through hole 4-1 and the second through hole 4-2 are combined pattern through holes, and the third through holes 4-3 and the third through hole 4-4 are elliptical through holes. The combined pattern is a combination of an ellipse and a square. The first through hole 4-1 contains a combination of a prism and a light-diffusing plate, the second through hole 4-2 contains a combination of a prism and a filter, the third through hole 4-3 contains a prism, and the fourth through hole 4-4 contains a combination of a prism, a light-diffusing plate, and a filter.

[0064] The combination of prism and filter includes not only a combination of a single prism and a single filter, but also a prism coated with a filter film.

[0065] The combination of prism, light diffuser and filter includes not only a combination of a single prism, a single light diffuser and a single filter, but also a combination of a prism coated with a filter film and a single light diffuser.

[0066] When using the biological microplate microscopic imaging optical path device provided in this embodiment to measure the liquid to be tested with a concave liquid surface, a clear image can be obtained.

[0067] Example 3

[0068] This embodiment provides a microscopic imaging optical path device for biological microplates. A schematic diagram of the structure of the microscopic imaging optical path device for biological microplates is shown below. Figure 1 As shown, it includes three components arranged in sequence: a light source assembly, an aperture assembly, and an imaging lens group; the central axis of the light source assembly, the central axis of the three imaging lens groups, and the central axis of the multifunctional aperture 4 coincide.

[0069] A schematic diagram of the structure of the light source assembly is shown below. Figure 2 As shown, it includes a light source base 5, a sealing ring 6, an LED light source 7, a fixing component 1, and a heat sink 8. The central axes of the light source base 5, the sealing ring 6, the LED light source 7, the fixing component 1, and the heat sink 8 are coincident. The sealing ring 6 and the fixing component 1 are used to fix the LED light source 7 to the light source base 5. The heat sink 8 is installed on the end face of the light source base 5 and contacts the LED light source 7 for heat dissipation of the LED light source 7.

[0070] The imaging lens assembly includes a lens barrel 2 and a lens assembly 3 fixed inside the lens barrel 2 by a fixing member 1. A schematic diagram of the structure of the lens assembly 3 is shown below. Figure 3 As shown, the principal axes of all lenses in lens group 3 coincide. Along the direction of light illumination, lens group 3 includes a first lens 3-1, a second lens 3-2, a third lens 3-3, a fourth lens 3-4, and a fifth lens 3-5 arranged sequentially. Specifically, the first lens 3-1 is a meniscus lens; the second lens 3-2 is a plano-convex lens; the third lens 3-3 is a plano-convex lens; the fourth lens 3-4 is a biconvex lens; and the fifth lens 3-5 is a meniscus lens. Light rays passing through the aperture sequentially enter the concave surface of the first lens 3-1, the plane of the second lens 3-2, the plane of the third lens 3-3, the convex surface of the fourth lens 3-4, and the convex surface of the fifth lens 3-5.

[0071] The aperture assembly is as follows: Figure 6 The multifunctional aperture 4 shown includes an aperture plate with four semi-circular through holes, which are evenly distributed around the center of the aperture plate. Specifically, the four semi-circular through holes are a first through hole 4-1, a second through hole 4-2, a third through hole 4-3, and a fourth through hole 4-4; wherein the first through hole 4-1 contains a combination of a prism and a filter, the second through hole 4-2 contains a combination of a prism and a filter, the third through hole 4-3 contains a combination of a prism and a filter, and the fourth through hole 4-4 contains a combination of a prism and a filter.

[0072] The combination of prism and filter includes not only a combination of a single prism and a single filter, but also a prism coated with a filter film.

[0073] When using the biological microplate microscopic imaging optical path device provided in this embodiment to measure the liquid to be tested with a concave liquid surface, a clear image can be obtained.

[0074] Example 4

[0075] This embodiment provides a microscopic imaging optical path device for biological microplates. A schematic diagram of the structure of the microscopic imaging optical path device for biological microplates is shown below. Figure 1 As shown, it includes three components arranged in sequence: a light source assembly, an aperture assembly, and an imaging lens group; the central axis of the light source assembly, the central axis of the three imaging lens groups, and the central axis of the multifunctional aperture 4 coincide.

[0076] A schematic diagram of the structure of the light source assembly is shown below. Figure 2 As shown, it includes a light source base 5, a sealing ring 6, an LED light source 7, a fixing component 1, and a heat sink 8. The central axes of the light source base 5, the sealing ring 6, the LED light source 7, the fixing component 1, and the heat sink 8 are coincident. The sealing ring 6 and the fixing component 1 are used to fix the LED light source 7 to the light source base 5. The heat sink 8 is installed on the end face of the light source base 5 and contacts the LED light source 7 for heat dissipation of the LED light source 7.

[0077] The imaging lens assembly includes a lens barrel 2 and a lens assembly 3 fixed inside the lens barrel 2 by a fixing member 1. A schematic diagram of the structure of the lens assembly 3 is shown below. Figure 3 As shown, the principal axes of all lenses in lens group 3 coincide. Along the direction of light illumination, lens group 3 includes a first lens 3-1, a second lens 3-2, a third lens 3-3, a fourth lens 3-4, and a fifth lens 3-5 arranged sequentially. Specifically, the first lens 3-1 is a meniscus lens; the second lens 3-2 is a plano-convex lens; the third lens 3-3 is a plano-convex lens; the fourth lens 3-4 is a biconvex lens; and the fifth lens 3-5 is a meniscus lens. Light rays passing through the aperture sequentially enter the concave surface of the first lens 3-1, the plane of the second lens 3-2, the plane of the third lens 3-3, the convex surface of the fourth lens 3-4, and the convex surface of the fifth lens 3-5.

[0078] The aperture assembly is as follows: Figure 7The multifunctional aperture 4 shown includes an aperture plate with four circular through holes, which are evenly distributed around the center of the aperture plate; moreover, the aperture diameters of the four circular through holes are different. Specifically, the four circular through holes are a first through hole 4-1, a second through hole 4-2, a third through hole 4-3, and a fourth through hole 4-4; wherein the first through hole 4-1 contains a combination of a prism and a filter, the second through hole 4-2 contains a combination of a prism and a filter, the third through hole 4-3 contains a combination of a prism and a filter, and the fourth through hole 4-4 contains a combination of a prism and a filter.

[0079] The combination of prism and filter includes not only a combination of a single prism and a single filter, but also a prism coated with a filter film.

[0080] When using the biological microplate microscopic imaging optical path device provided in this embodiment to measure the liquid to be tested with a concave liquid surface, a clear image can be obtained.

[0081] Example 5

[0082] This embodiment provides a microscopic imaging optical path device for biological microplates. A schematic diagram of the structure of the microscopic imaging optical path device for biological microplates is shown below. Figure 1 As shown, it includes three components arranged in sequence: a light source assembly, an aperture assembly, and an imaging lens group; the central axis of the light source assembly, the central axis of the three imaging lens groups, and the central axis of the multifunctional aperture 4 coincide.

[0083] A schematic diagram of the structure of the light source assembly is shown below. Figure 2 As shown, it includes a light source base 5, a sealing ring 6, an LED light source 7, a fixing component 1, and a heat sink 8. The central axes of the light source base 5, the sealing ring 6, the LED light source 7, the fixing component 1, and the heat sink 8 are coincident. The sealing ring 6 and the fixing component 1 are used to fix the LED light source 7 to the light source base 5. The heat sink 8 is installed on the end face of the light source base 5 and contacts the LED light source 7 for heat dissipation of the LED light source 7.

[0084] The imaging lens assembly includes a lens barrel 2 and a lens assembly 3 fixed inside the lens barrel 2 by a fixing member 1. A schematic diagram of the structure of the lens assembly 3 is shown below. Figure 3As shown, the principal axes of all lenses in lens group 3 coincide. Along the direction of light illumination, lens group 3 includes a first lens 3-1, a second lens 3-2, a third lens 3-3, a fourth lens 3-4, and a fifth lens 3-5 arranged sequentially. Specifically, the first lens 3-1 is a meniscus lens; the second lens 3-2 is a plano-convex lens; the third lens 3-3 is a plano-convex lens; the fourth lens 3-4 is a biconvex lens; and the fifth lens 3-5 is a meniscus lens. Light rays passing through the aperture sequentially enter the concave surface of the first lens 3-1, the plane of the second lens 3-2, the plane of the third lens 3-3, the convex surface of the fourth lens 3-4, and the convex surface of the fifth lens 3-5.

[0085] The aperture assembly is as follows: Figure 9 The multifunctional aperture 4 shown includes an aperture plate with eight through holes, which are evenly distributed around the center of the aperture plate. Specifically, the eight through holes are designated as the first through hole 4-1, the second through hole 4-2, the third through hole 4-3, the fourth through hole 4-4, the fifth through hole 4-5, the sixth through hole 4-6, the seventh through hole 4-7, and the eighth through hole 4-8. The first through hole 4-1, the second through hole 4-2, the third through hole 4-3, and the fourth through hole 4-4 are semi-circular through holes, the fifth through hole 4-5 and the sixth through hole 4-6 are elliptical through holes, and the seventh through hole 4-7 and the eighth through hole 4-8 are circular through holes.

[0086] A combination of prism and filter is provided in the first through hole 4-1, the second through hole 4-2, the third through hole 4-3 and the fourth through hole 4-4; a prism is provided in the fifth through hole 4-5 and the sixth through hole 4-6; and a light-diffusing plate is provided in the seventh through hole 4-7 and the eighth through hole 4-8.

[0087] The combination of prism and filter includes not only a combination of a single prism and a single filter, but also a prism coated with a filter film.

[0088] When using the biological microplate microscopic imaging optical path device provided in this embodiment to measure the liquid to be tested with a concave liquid surface, a clear image can be obtained.

[0089] Comparative Example 1

[0090] This comparative example provides a biological microplate microscopic imaging optical path device, which includes a light source assembly, an aperture assembly, and an imaging lens group arranged in sequence; the central axis of the light source assembly, the central axis of the three imaging lens groups, and the central axis of the multifunctional aperture 4 coincide.

[0091] Compared with Example 1, the aperture assembly provided in this comparative example does not include functional components, but is otherwise the same as in Example 1.

[0092] Application Example 1

[0093] This application example provides an application of the biological microplate microscopic imaging optical path device provided in Example 1 to examine a sample with a concave liquid surface. The concave liquid surface is divided into regions A, B, C and D, which correspond to the first through hole 4-1, the second through hole 4-2, the third through hole 4-3 and the fourth through hole 4-4, respectively.

[0094] First, the first through-hole 4-1 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the first through-hole 4-1 and illuminate region A, thus obtaining a detection image of that region. Then, the second through-hole 4-2 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the second through-hole 4-2 and illuminate region B, thus obtaining a detection image of that region. Next, the third through-hole 4-3 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the third through-hole 4-3 and illuminate region C, thus obtaining a detection image of that region. Then, the fourth through-hole 4-4 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the fourth through-hole 4-4 and illuminate region D, thus obtaining a detection image of that region. Using conventional image synthesis software in the art, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample to be tested.

[0095] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0096] The final synthesized image is as follows Figure 11 As shown, by Figure 11 It can be seen that the edges of the resulting synthesized image are clear and the field of view is distinct.

[0097] Application Example 2

[0098] This application example provides an application of the biological microplate microscopic imaging optical path device provided in Example 2 to examine a sample with a concave liquid surface. The concave liquid surface is divided into regions A, B, C and D, which correspond to the first through hole 4-1, the second through hole 4-2, the third through hole 4-3 and the fourth through hole 4-4, respectively.

[0099] Light generated by the light source component simultaneously passes through the first through-hole 4-1, the second through-hole 4-2, the third through-hole 4-3, and the fourth through-hole 4-4, illuminating the corresponding regions A, B, C, and D, thereby obtaining detection images for each region. Adjacent regions may have overlapping areas. Using conventional image synthesis software, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample under test.

[0100] First, the first through-hole 4-1 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the first through-hole 4-1 and illuminate region A, thus obtaining a detection image of that region. Then, the second through-hole 4-2 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the second through-hole 4-2 and illuminate region B, thus obtaining a detection image of that region. Next, the third through-hole 4-3 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the third through-hole 4-3 and illuminate region C, thus obtaining a detection image of that region. Then, the fourth through-hole 4-4 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the fourth through-hole 4-4 and illuminate region D, thus obtaining a detection image of that region. Using conventional image synthesis software in the art, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample to be tested.

[0101] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0102] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0103] Application Example 3

[0104] This application example provides an application of the biological microplate microscopic imaging optical path device provided in Example 3 to examine a sample with a concave liquid surface. The concave liquid surface is divided into regions A, B, C and D, which correspond to the first through hole 4-1, the second through hole 4-2, the third through hole 4-3 and the fourth through hole 4-4, respectively.

[0105] Light generated by the light source component simultaneously passes through the first through-hole 4-1, the second through-hole 4-2, the third through-hole 4-3, and the fourth through-hole 4-4, illuminating the corresponding regions A, B, C, and D, thereby obtaining detection images for each region. Adjacent regions may have overlapping areas. Using conventional image synthesis software, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample under test.

[0106] First, the first through-hole 4-1 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the first through-hole 4-1 and illuminate region A, thus obtaining a detection image of that region. Then, the second through-hole 4-2 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the second through-hole 4-2 and illuminate region B, thus obtaining a detection image of that region. Next, the third through-hole 4-3 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the third through-hole 4-3 and illuminate region C, thus obtaining a detection image of that region. Then, the fourth through-hole 4-4 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the fourth through-hole 4-4 and illuminate region D, thus obtaining a detection image of that region. Using conventional image synthesis software in the art, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample to be tested.

[0107] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0108] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0109] Application Example 4

[0110] This application example provides an application of the biological microplate microscopic imaging optical path device provided in Example 4 to examine a sample with a concave liquid surface. The concave liquid surface is divided into regions A, B, C and D, which correspond to the first through hole 4-1, the second through hole 4-2, the third through hole 4-3 and the fourth through hole 4-4, respectively.

[0111] First, the first through-hole 4-1 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the first through-hole 4-1 and illuminate region A, thus obtaining a detection image of that region. Then, the second through-hole 4-2 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the second through-hole 4-2 and illuminate region B, thus obtaining a detection image of that region. Next, the third through-hole 4-3 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the third through-hole 4-3 and illuminate region C, thus obtaining a detection image of that region. Then, the fourth through-hole 4-4 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the fourth through-hole 4-4 and illuminate region D, thus obtaining a detection image of that region. Using conventional image synthesis software in the art, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample to be tested.

[0112] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light; the apertures of the first through-hole 4-1, the second through-hole 4-2, the third through-hole 4-3 and the fourth through-hole 4-4 are different, thereby realizing the adjustment of the light flux in different areas.

[0113] Application Example 5

[0114] This application example provides an application of the biological microplate microscopic imaging optical path device provided in Example 5 to examine a sample with a concave liquid surface. The concave liquid surface is divided into regions A, B, C, D, E, F, G, and H, where regions A, B, C, D, E, F, G, and H correspond to the first through hole 4-1, the second through hole 4-2, the third through hole 4-3, the fourth through hole 4-4, the fifth through hole 4-5, the sixth through hole 4-6, the seventh through hole 4-7, and the eighth through hole 4-8, respectively.

[0115] Light generated by the light source assembly simultaneously passes through the first through-hole 4-1, the second through-hole 4-2, the third through-hole 4-3, the fourth through-hole 4-4, the fifth through-hole 4-5, the sixth through-hole 4-6, the seventh through-hole 4-7, and the eighth through-hole 4-8, illuminating the corresponding regions A, B, C, D, E, F, G, and H, thereby obtaining detection images for each region. Adjacent regions may have overlapping areas. Using conventional image synthesis software, the detection images obtained from regions A, B, C, D, E, F, G, and H are synthesized to complete the detection of the sample to be tested.

[0116] First, the first through-hole 4-1 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the first through-hole 4-1 and illuminate region A, obtaining a detection image of that region. Then, the second through-hole 4-2 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the second through-hole 4-2 and illuminate region B, obtaining a detection image of that region. Next, the third through-hole 4-3 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the third through-hole 4-3 and illuminate region C, obtaining a detection image of that region. Then, the fourth through-hole 4-4 is positioned between the light source assembly and the imaging lens group (3 components), allowing light generated by the light source assembly to pass through the fourth through-hole 4-4 and illuminate region D, obtaining a detection image of that region. This process is repeated for regions E, F, G, and H. Using conventional image synthesis software, the detection images obtained from regions A, B, C, D, E, F, G, and H are synthesized to complete the detection of the sample under test.

[0117] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0118] The light homogenizer in the through-hole can make the light from the light source more uniform, thereby improving the optical path; the prism can appropriately change the angle of the light from the light source; the filter can intercept the required monochromatic light for fluorescence experiments other than white light.

[0119] Comparative Application Example 1

[0120] This comparative application example provides an application of using the biological microplate microscopic imaging optical path device provided in Comparative Example 1 to examine a sample with a concave liquid surface. The concave liquid surface is divided into regions A, B, C and D, where regions A, B, C and D correspond to the first through hole 4-1, the second through hole 4-2, the third through hole 4-3 and the fourth through hole 4-4, respectively.

[0121] Light generated by the light source component simultaneously passes through the first through-hole 4-1, the second through-hole 4-2, the third through-hole 4-3, and the fourth through-hole 4-4, illuminating the corresponding regions A, B, C, and D, thereby obtaining detection images for each region. Adjacent regions may have overlapping areas. Using conventional image synthesis software, the detection images obtained from regions A, B, C, and D are synthesized to complete the detection of the sample under test.

[0122] The synthesized image obtained in this comparative application example is as follows: Figure 12 As shown, by Figure 12 It is known that it is impossible to obtain an image of the edge of the concave liquid surface.

[0123] In summary, this invention achieves a compact structure for the aperture plate by creating at least four through holes and incorporating different functional components within these holes. The light homogenizer ensures more uniform light distribution from the light source, thus improving the optical path. The prism allows for appropriate changes in the angle of the light emitted from the light source. The filter intercepts the desired monochromatic light for fluorescence experiments involving light other than white light. This invention achieves the "multi-functionality" of the aperture by incorporating different functional components within the through holes. Furthermore, this invention combines the light source assembly, aperture assembly, and imaging lens assembly into a biological microplate display. In the micro-imaging optical path device, when measuring a sample with a concave liquid surface using the aforementioned biological microplate micro-imaging optical path device, the through holes of the multifunctional aperture correspond to the liquid surface area of ​​the sample, that is, n through holes divide the liquid surface area into n regions. An optical fiber of the imaging lens assembly is introduced through each through hole to illuminate the corresponding region, where n is an integer ≥ 4. The biological microplate micro-imaging optical path device, through the setting of the multifunctional aperture, enables those skilled in the art to adjust the optical path according to the condition of each region of the sample, thereby making the final synthesized image of each region clear and complete.

[0124] The applicant declares that the above description is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention fall within the protection and disclosure scope of the present invention.

Claims

1. A method for accurately measuring different test samples with different concave meniscus conditions, characterized in that, The method uses a biological microplate microscopic imaging optical path device to measure a sample with a concave liquid surface, including: positioning one of at least four through holes of a multifunctional aperture between the light source assembly and the imaging lens assembly, and observing the relevant area through the shape of the through hole and its functional components; after observation, switching the other through holes in the multifunctional aperture, and using the switched through holes to observe the relevant area of ​​the concave liquid surface; repeating the above steps until the entire area of ​​the concave liquid surface has been observed, and then using conventional image processing software to integrate the observed images to obtain a clear image of the sample with a concave liquid surface. The biological microplate microscopic imaging optical path device includes a light source assembly, an aperture assembly, and an imaging lens assembly arranged in sequence. The aperture assembly is a multi-functional aperture; The central axis of the light source assembly coincides with the central axis of the imaging lens assembly; The multifunctional aperture includes an aperture plate with at least four through holes; different functional components are provided in the through holes, and the functional components include any one or a combination of at least two of the following: a light diffuser, a prism, or a filter; The through-holes of the multifunctional aperture correspond to the liquid surface area of ​​the sample to be tested; the n through-holes of the multifunctional aperture divide the liquid surface area of ​​the sample to be tested into n regions, where n is an integer ≥ 4.

2. The method according to claim 1, characterized in that, The shape of the through hole includes any one of the following: circle, ellipse, semicircle, or combination of shapes; the combination of shapes includes a combination of semicircle and square or a combination of ellipse and square.

3. The method according to claim 1, characterized in that, The diameters of the at least four through holes are different.

4. The method according to claim 1, characterized in that, The at least four through holes are evenly distributed with the center of the aperture plate as the center.

5. The method according to claim 1, characterized in that, The light source assembly includes a light source holder, a sealing ring, an LED light source, a fixing component, and a heat sink; The sealing ring and the fastener are used to fix the LED light source to the light source holder; The heat sink is installed on the end face of the light source holder and in contact with the LED light source for heat dissipation of the LED light source.

6. The method according to claim 5, characterized in that, The central axes of the light source holder, sealing ring, LED light source, fixing component, and heat sink are coincident.

7. The method according to claim 1, characterized in that, The imaging lens assembly includes a lens barrel and a lens group fixed inside the lens barrel by a fixing member.

8. The method according to claim 7, characterized in that, The principal axes of all lenses in the lens group coincide.

9. The method according to claim 8, characterized in that, Along the direction of light illumination, the lens group includes a first lens, a second lens, a third lens, a fourth lens, and a fifth lens arranged sequentially.

10. The method according to claim 9, characterized in that, The first lens is a meniscus lens; the second lens is a plano-convex lens; the third lens is a plano-convex lens; the fourth lens is a biconvex lens; and the fifth lens is a meniscus lens. Light rays passing through the aperture sequentially enter the concave surface of the first lens, the flat surface of the second lens, the flat surface of the third lens, the convex surface of the fourth lens, and the convex surface of the fifth lens.

Images