A fungicidal composition containing trifloxystrobin

Abstract

The application discloses a kind of trifloxystrobin-containing fungicidal composition and application thereof, effective component includes trifloxystrobin and benzovindifel, the weight ratio of trifloxystrobin and benzovindifel is 1:80~80:1, preferably 1:50~50:1.The sum of the weight of benzovindifel accounts for 1%~90% of the composition, preferably 10%~60%.The present composition can be configured into an agriculturally permissible dosage form.The present application is reasonable in component, has good fungicidal effect, and its activity and fungicidal effect are not the simple superposition of each component.Compared with single agent, in addition to having significant fungicidal effect, it is good to crop safety.The present application has significant control effect on powdery mildew.

Description

Technical Field

[0001] This invention relates to a bactericidal composition, specifically a bactericidal composition containing oxime ester, belonging to the field of pesticide compound technology. Background Technology

[0002] Trifloxystrobin (CAS No.: 141517-21-7, chemical name: (E)-methoxyimino)-{(E)-α-[1-(α,α,α-trifluorom-tolyl)ethyleneaminooxy]o-tolyl}methyl acetate) is a methoxypropylene ester fungicide and an extraquinone inhibitor. It inhibits mitochondrial respiration by blocking electron transfer at the cytochrome bc1Qo center. It is a semi-systemic, broad-spectrum fungicide with protective properties. Specifically, it achieves redistribution of the agent on the plant through the movement of surface vapor and surface water. It is mainly used for the control of diseases of Ascomycetes, Deuteromycetes, Basidiomycetes, and Oomycetes.

[0003] Ectafenone (CAS No.: 220899-03-6, chemical name: (3-bromo-6'-methoxy-2'-methylphenyl)-(2,3,4-trimethoxy-6-methylphenyl) methyl ketone) is a benzophenone fungicide with preventive, curative and antispore activities; it inhibits the growth of both germ tubes and hyphae; and it can interfere with the establishment and maintenance of polar actin tissue.

[0004] Currently, there are no literature reports on the use of azoxystrobin and benomyl in the control of powdery mildew. Further experiments have shown that mixing azoxystrobin and benomyl, which have different mechanisms of action, has a significant synergistic effect, which can significantly improve the control effect of powdery mildew, reduce the amount of pesticide used, reduce the control cost, and has high safety for crops. Summary of the Invention

[0005] The purpose of this invention is to provide a composition and to prepare it into a fungicide composition with good bactericidal effect, low application cost, and safety for crops.

[0006] Another object of the present invention is to provide a formulation of a bactericidal composition containing oxadiazon and benomyl.

[0007] Another object of the present invention is to provide the use of the above composition in the prevention and control of powdery mildew in crops.

[0008] The technical solution of the present invention is as follows:

[0009] A bactericidal composition containing oxime ester, comprising oxime ester and benomyl;

[0010] The bactericidal composition wherein the weight ratio of oxadiazon to benomyl is 1:80 to 80:1;

[0011] Furthermore, in the bactericidal composition, the weight ratio of oxadiazon to benomyl is 1:50 to 50:1;

[0012] Furthermore, in the bactericidal composition, the weight ratio of oxadiazon to benomyl is 1:20 to 20:1;

[0013] Furthermore, in the bactericidal composition, the weight ratio of oxadiazon to benomyl is 1:10 to 10:1;

[0014] Furthermore, in the bactericidal composition, the weight ratio of oxadiazon to benomyl is 1:3 to 3:1;

[0015] The bactericidal composition wherein the total weight of the active ingredients oxadiazon and benomyl accounts for 1% to 90% of the composition, preferably 10% to 60%.

[0016] The bactericidal composition can be prepared into an agriculturally permissible formulation;

[0017] Furthermore, the composition includes an active ingredient and pesticide formulation adjuvants;

[0018] Furthermore, the pesticide formulation auxiliary components include a carrier and adjuvants;

[0019] Furthermore, the carrier is any one, two, or three of water, solvent, or filler, with deionized water being preferred;

[0020] Further, the solvent is selected from one or more of the following: N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate, or acetonitrile.

[0021] Furthermore, the filler is selected from one or more of the following: kaolin, diatomaceous earth, bentonite, attapulgite, silica, starch, or light calcium carbonate;

[0022] Furthermore, the additives include at least one surfactant, and other functional additives such as antifreeze, thickener, stabilizer, disintegrant, and defoamer may be added depending on the application and requirements.

[0023] Furthermore, the surfactant is selected from one or more of emulsifiers, dispersants, wetting agents, or penetrants;

[0024] Furthermore, the other functional additives are selected from one or more of antifreeze, thickeners, stabilizers, disintegrants, or defoamers;

[0025] Furthermore, the emulsifier is selected from agricultural emulsion 500# (alkylbenzene sulfonate calcium), OP series phosphate esters (nonylphenol polyoxyethylene ether phosphate ester), 600# phosphate ester (phenylphenol polyoxyethylene ether phosphate ester), styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether magnesium disulfonate, triethanolamine salt, agricultural emulsion 400# (benzyl dimethylphenol polyoxyethylene ether), agricultural emulsion 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ningxia emulsion 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether). The mixture consists of one or more of the following: ethyl ether, phenylethyl phenol polyoxyethylene polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), alkyl aryl polyoxyethylene polyoxypropylene ether, Span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether), or AEO series (fatty alcohol polyoxyethylene ether).

[0026] Further, the dispersant is selected from one or more of the following: polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylbenzene sulfonate calcium, naphthalene sulfonate formaldehyde polymer sodium salt, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, or glycerol fatty acid ester polyoxyethylene ether.

[0027] Furthermore, the wetting agent is selected from one or more of the following: sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, pull-opening powder BX, wetting and penetrating agent F, soapberry powder, silkworm excrement, or soapberry powder;

[0028] Furthermore, the penetrant is selected from one or more of the following: penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone, or organosilicon;

[0029] Furthermore, the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea, or a mixture thereof;

[0030] Furthermore, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose, or magnesium aluminum silicate;

[0031] Furthermore, the stabilizer is selected from one or more of the following: epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0032] Furthermore, the disintegrant is selected from one or more of the following: bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate;

[0033] Furthermore, the defoamer is selected from one or more of the following: silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds, or C8-C10 fatty alcohol compounds;

[0034] All of the above substances are commercially available.

[0035] The compositions of the present invention can be formulated into pesticide-acceptable formulations, including solid formulations, liquid formulations, seed treatment formulations, and other formulations, according to methods known to those skilled in the art.

[0036] Further, solid dosage forms include powders, dispersible tablets, granules, soluble powders, soluble granules, soluble tablets, tablets, milk powders, milk granules, water-dispersible granules, wettable powders, microcapsule granules, powders, large granules, and water-dispersible tablets;

[0037] Furthermore, the liquid formulation includes microcapsule suspensions, dispersible liquids, emulsifiable concentrates, emulsions, granules, oil emulsions, powders, water emulsions, microemulsions, dispersible oil suspensions, oil-dispersible powders, suspensions, suspension emulsions, soluble concentrates, and ultra-low volume liquids.

[0038] Furthermore, the seed treatment formulation is a suspension seed coating agent, a seed treatment dry powder, a seed treatment dispersible powder, a seed treatment suspension, or a seed treatment liquid.

[0039] Furthermore, other formulations include bait, concentrated bait, gaseous formulations, aerosols, and smoke agents;

[0040] Furthermore, the preferred dosage form is a wettable powder, a water-dispersible granule, a suspension, a microemulsion, an emulsifiable concentrate, or an aqueous emulsion;

[0041] Furthermore, the composition is prepared into a wettable powder, and the preferred components and contents are: 0.1% to 80% oxadiazon, 0.1% to 80% benomyl, 2% to 10% dispersant, 2% to 10% wetting agent, and filler to make up the balance;

[0042] Furthermore, the components and contents of the composition prepared into water-dispersible granules are preferably as follows: 0.1% to 80% oxadiazon, 0.1% to 80% benomyl, 3% to 12% dispersant, 1% to 8% wetting agent, 1% to 10% disintegrant, and filler to make up the balance;

[0043] Furthermore, the components and contents of the composition prepared into a suspension are preferably as follows: 0.1%–80% oxadiazon, 0.1%–80% benomyl, 2%–10% dispersant, 2%–10% wetting agent, 0.01%–2% defoamer, 0–2% thickener, 0–8% antifreeze agent, and deionized water to make up the balance;

[0044] Furthermore, the composition is prepared into a microemulsion, and the preferred components and contents are: 0.1%–80% oxadiazon, 0.1%–80% benomyl, 10%–25% solvent, 5%–15% emulsifier, and deionized water to make up the balance;

[0045] Furthermore, the components and contents of the composition prepared into an emulsifiable concentrate are preferably: 0.1% to 80% oxime ester, 0.1% to 80% benomyl, 5% to 15% emulsifier, and solvent to make up the balance;

[0046] Furthermore, the components and contents of the composition prepared into an emulsion are preferably as follows: 0.1% to 80% oxadiazon, 0.1% to 80% benomyl, 0% to 5% disintegrant, 5% to 15% emulsifier, and deionized water to make up the balance;

[0047] The present invention also discloses the use of the bactericidal composition described above for the prevention and control of crop diseases;

[0048] Furthermore, the disease in question is specifically powdery mildew;

[0049] Furthermore, the crop mentioned is cucumber and / or strawberry;

[0050] Furthermore, the crop diseases mentioned are cucumber powdery mildew and / or strawberry powdery mildew.

[0051] The present invention also discloses a method for preventing and controlling crop diseases, which involves applying the fungicidal composition as described above to the growing area and / or leaves and / or stems of the crop;

[0052] Furthermore, the disease in question is specifically powdery mildew;

[0053] Furthermore, the crop mentioned is cucumber and / or strawberry;

[0054] Furthermore, the crop diseases mentioned are cucumber powdery mildew and / or strawberry powdery mildew.

[0055] Compared with the prior art, the present invention has the following beneficial effects: the bactericidal composition of the present invention can produce a high synergistic effect, significantly improve the control effect of powdery mildew, reduce the amount of pesticide used, reduce the control cost, and has high safety for crops. Detailed Implementation

[0056] The present invention will be described in more detail below with reference to the embodiments. The implementation of the present invention is not limited to the following embodiments, and any modifications or alterations to the implementation of the present invention fall within the protection scope of the present invention. Unless otherwise specified, the methods in the following embodiments are conventional methods in the art.

[0057] Formulation preparation case

[0058] Example 1: 10% azoxystrobin·benzoxystrobin wettable powder (5:5)

[0059] Formula: 5% oxime ester, 5% benomyl, 3% calcium lignosulfonate, 5% sodium alkylnaphthalene formaldehyde condensate sulfonate, 2% sodium alkyl sulfonate, clay to make up the balance;

[0060] Preparation method: The active ingredients oxadiazon, benomyl, dispersant, wetting agent and filler are mixed according to the above formula, stirred evenly in a stirring tank, pulverized by an air jet mill and then mixed evenly to prepare the wettable powder formulation of the composition of the present invention.

[0061] Example 2: 15% azoxystrobin·benzoxystrobin wettable powder (10:5)

[0062] Formula: 10% oxime ester, 5% benomyl, 4% alkylphenol polyoxyethylene ether calcium sulfonate, 4% alkyl naphthalene sulfonate formaldehyde condensate, 2% alkyl naphthalene sulfonate sodium, talc to make up the balance;

[0063] Preparation method: Same as in Example 1.

[0064] Example 3: 25% azoxystrobin·benzoxystrobin wettable powder (10:15)

[0065] Formula: 10% oxime ester, 15% benomyl, 5% sodium alkylnaphthalene formaldehyde condensate sulfonate, 3% sodium polycarboxylate, 3% sodium dodecyl sulfate, kaolin to make up the balance;

[0066] Preparation method: Same as in Example 1.

[0067] Example 4: 10% azoxystrobin·benzoxystrobin water-dispersible granules (4:6)

[0068] Formula: 4% oxime ester, 6% benomyl, 3% polycarboxylate GY-D06, 3% sodium alkyl naphthalene sulfonate formaldehyde condensate, 3% sodium dodecylbenzene sulfonate, 5% sodium naphthalene sulfonate, 2% sodium carboxymethyl cellulose, with attapulgite clay to make up the balance;

[0069] The preparation method is as follows: the active ingredients oxadiazon, benomyl, dispersant, wetting agent, disintegrant and filler are mixed evenly, pulverized by ultra-micro airflow pulverizer, kneaded, and then added to fluidized bed granulation dryer for granulation, drying, sieving and sampling analysis, so as to prepare the water-dispersible granule formulation of the composition of the present invention.

[0070] Example 5: 30% azoxystrobin·benzoxystrobin water-dispersible granules (15:15)

[0071] Formula: 15% oxamyl ester, 15% benomyl, 2% sodium alkyl naphthalene sulfonate, 4% sodium lignosulfonate, 2% sodium dibutyl naphthalene sulfonate, 3% sodium dodecyl sulfate, 1% urea, 2% polyvinyl alcohol, clay to make up the balance;

[0072] Preparation method: Same as in Example 4.

[0073] Example 6: 15% azoxystrobin·benzoxystrobin water-dispersible granules (10:5)

[0074] Formula: 10% oxamyl ester, 5% benomyl, 5% dispersant NNO, 3% sodium lignosulfonate, 4% sodium dibutylnaphthalenesulfonate, 3% sodium dodecylbenzenesulfonate, 1.5% sodium bicarbonate, 2% starch, talc to make up the balance;

[0075] Preparation method: Same as in Example 4.

[0076] Example 7: 20% azoxystrobin·benzoxystrobin suspension (10:10)

[0077] Formula: 10% oxamyl ester, 10% benomyl, 3% sodium lignosulfonate, 3% phenethylphenol polyoxyethylene ether phosphate, 4% sodium dodecyl sulfonate, 0.5% carboxymethyl cellulose, 3% polyethylene glycol, 0.2% isothiazolinone, 1% octanol, deionized water to make up the balance;

[0078] Preparation method: Mix the adjuvants and mix them evenly by high-speed shearing. Add the active ingredients oxadiazon and benomyl. Grind the mixture in a ball mill for 2-3 hours until the particle diameter is less than 5 mm. The suspension formulation of the composition of the present invention can then be prepared.

[0079] Example 8: 30% azoxystrobin·benzoxystrobin suspension (10:20)

[0080] Formula: 10% oxamyl ester, 20% benomyl, 3% sodium lignosulfonate, 3% sodium naphthalenesulfonate, 4% sodium dodecyl sulfonate, 0.5% carboxymethyl cellulose, 3% magnesium aluminum silicate, 0.2% propylene glycol, 1% hexanol, deionized water to make up the balance;

[0081] Preparation method: Same as in Example 7.

[0082] Example 9: 40% azoxystrobin·benzoxystrobin suspension (10:30)

[0083] Formula: 10% oxime ester, 30% benomyl, 3% phenethylphenol polyoxyethylene ether phosphate, 4% sodium dodecyl sulfonate, 0.5% carboxymethyl cellulose, 3% magnesium aluminum silicate, 1% octanol, deionized water to make up the balance;

[0084] Preparation method: Same as in Example 7.

[0085] Example 10: 20% azoxystrobin·benzoxystrobin microemulsion (10:10)

[0086] Formula: 10% azoxystrobin, 10% benomyl, 3% agricultural emulsion 1601, 3% calcium dodecylbenzenesulfonate, 3% methanol, 3% xylene, 1% epichlorohydrin, deionized water to make up the balance;

[0087] Preparation method: The active ingredients oxadiazon and benomyl are completely dissolved in solvent and co-solvent, then other adjuvants are added and mixed evenly. Finally, water is added and stirred thoroughly to prepare the microemulsion formulation of the composition of the present invention.

[0088] Example 11: 40% azoxystrobin·benzoxystrobin microemulsion (10:30)

[0089] Formula: 10% azoxystrobin, 30% benomyl, 5% agricultural emulsion 600#, 3% isopropanol, 3% toluene, 1% triphenyl phosphite, deionized water to make up the balance;

[0090] Preparation method: Same as in Example 10.

[0091] Example 12: 10% azoxystrobin·benzoxystrobin microemulsion (10:5)

[0092] Formula: 10% azoxystrobin, 5% benomyl, 3% agricultural emulsifier 700#, 3% agricultural emulsifier 2201, 3% n-butanol, 2% toluene, 1% epichlorohydrin, deionized water to make up the balance;

[0093] Preparation method: Same as in Example 10.

[0094] Example 13: 20% azoxystrobin·benzoxystrobin EC (10:10)

[0095] Formula: 10% azoxystrobin, 10% benomyl, 3% agricultural emulsifier 600#, 5% Span-60#, 2% methanol, 2% epichlorohydrin, xylene to make up the balance;

[0096] Preparation method: The active ingredients oxamyl ester and benomyl are added to the carrier, and surfactants and other functional adjuvants are added thereto. The mixture is stirred and mixed evenly in a stirring mixing tank to prepare the emulsifiable concentrate formulation of the composition of the present invention.

[0097] Example 14: 30% azoxystrobin·benzoxystrobin EC (10:20)

[0098] Formula: 10% azoxystrobin, 20% benomyl, 5% emulsifier T-60, 5% agricultural emulsion 1601#, 2% cyclohexanone, 2% acetic anhydride, toluene to make up the balance;

[0099] Preparation method: Same as in Example 13.

[0100] Example 15: 50% azoxystrobin·benzoxystrobin EC (25:25)

[0101] Formula: 25% azoxystrobin, 25% benomyl, 5% emulsifier T-60, 5% agricultural emulsion 1601#, 2% acetone, 2% triphenyl phosphite, biodiesel to make up the balance;

[0102] Preparation method: Same as in Example 13.

[0103] Example 16: 30% azoxystrobin·benzoxystrobin water-in-oil emulsion (10:20)

[0104] Formula: 10% azoxystrobin, 20% benomyl, 5% agricultural emulsion 1601, 2% toluene, 1% triphenyl phosphite, 2% propylene glycol, 5% bentonite, 0.2% sodium benzoate, deionized water to make up the balance;

[0105] Preparation method: The active ingredients oxamyl ester, benomyl, and adjuvants are added together to dissolve into a uniform oil phase. While stirring at high speed in a reaction vessel, the oil phase is added to the aqueous phase. Water is slowly added until the phase inversion point is reached. A shearing machine is turned on for high-speed shearing, and the remaining water is added. Shearing is carried out for about half an hour to prepare the water emulsion formulation of the composition of the present invention.

[0106] Example 17: 20% azoxystrobin·benzoxystrobin water-in-oil emulsion (10:10)

[0107] Formula: 10% azoxystrobin, 10% benomyl, 6% Tween-60#, 2% cyclohexanone, 1% epoxidized soybean oil, 2% ethylene glycol, 5% polyvinyl alcohol, 0.2% methanol, deionized water to make up the balance;

[0108] Preparation method: Same as in Example 16.

[0109] Example 18: 40% azoxystrobin·benzoxystrobin water-in-oil emulsion (30:10)

[0110] Formula: 30% azoxystrobin, 10% benomyl, 2% Span-60#, 4% agricultural emulsion 400#, 2% xylene, 2% sodium chloride, 0.2% benzoic acid, deionized water to make up the balance;

[0111] Preparation method: Same as in Example 16.

[0112] Active Case

[0113] The following analysis of the combined effects of oxadiazon and benomyl will illustrate the types of combined effects of these mixtures on powdery mildew in crops.

[0114] Example 19: Determination of the combined effect of a mixture of oxadiazon and benomyl on cucumber powdery mildew.

[0115] Test basis: NY / T 1156.11-2008 "Guidelines for Indoor Bioassay Testing of Pesticides - Fungicides Part 11: Pot Test for Control of Powdery Mildew in Cucurbits"

[0116] Test target: Cucumber powdery mildew (Erysiphe cichoracearum), cucumber potted plants cultivated to the 2-4 true leaf stage, numbered for future use.

[0117] Test reagents: 98% azoxystrobin technical grade and 98% benomyl technical grade, provided by the R&D Center of Hailier Pharmaceutical Group Co., Ltd.

[0118] Preparation of spore suspension: Fresh spores from plant leaves infested with powdery mildew were washed with purified water containing a small amount of Tween-80, filtered through double-layered gauze, and prepared to a spore concentration of 1×10⁻⁶. 5 A suspension of 1 spore / mL is prepared for later use.

[0119] Preparation of the reagent: Weigh 0.1053g of azoxystrobin technical and benzoyl peroxide technical respectively, and dissolve them in 2.00g of N-dimethylformamide respectively. Then dilute them with 0.1% Tween-80 aqueous solution and make up to 100mL. Stir well to prepare a 1000mg / L stock solution.

[0120] Blank control stock solution: Weigh 2.00 g of N-dimethylformamide, dilute with 0.1% Tween-80 aqueous solution and bring the volume to 100 mL. Set up 5 treatment concentration gradients with the prepared solution for later use.

[0121] Chemical treatment: Spray the solution evenly onto the leaves until they are completely wet, and allow the solution to air dry naturally before use. Each treatment consists of 3 pots, with 4 replicates, and a blank control is included.

[0122] Inoculation and culture: Inoculate with spore suspension by spray. After inoculation, the test material is allowed to air dry naturally, and then transferred to a greenhouse for culture at a temperature of 20℃-24℃ for 7 days.

[0123] Investigation: When the disease rate of the blank control reaches 80% or more, conduct a graded investigation of the disease incidence in each treatment. At least 30 leaves should be investigated for each treatment. The grading criteria are as follows:

[0124] Grade 0: No lesions;

[0125] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0126] Grade 3: Lesions cover 6% to 10% of the total leaf area;

[0127] Level 5: Lesions cover 11%–20% of the total leaf area;

[0128] Level 7: Lesions cover 21%–40% of the total leaf area;

[0129] Level 9: The lesion area accounts for more than 40% of the total leaf area.

[0130] Data statistics and analysis:

[0131] Calculate the disease index, and then use the disease index to calculate the relative efficacy of each concentration.

[0132] Disease index = [∑(number of diseased leaves at each level × relative level) / (total number of leaves surveyed × 9)] × 100

[0133] Prevention and control efficacy (%) = [(Control disease index - Treatment disease index) / Control disease index] × 100

[0134] The EC value of each drug was calculated using DPS software. 50 Values ​​and corresponding b-values, correlation coefficients.

[0135] SR=EC 50 (Theoretical value) / EC 50 (Observations)

[0136] EC 50 (Theoretical value) = (a+b) / (a / A's EC) 50 EC +b / B 50 )

[0137] Where: A is oxadiazon, B is benomyl; a is the percentage content of oxadiazon in the combination, and b is the percentage content of benomyl in the combination.

[0138] The synergistic ratio (SR) of two drugs in different ratios was calculated using the Wadley method. SR < 0.5 indicates antagonistic effect, 0.5 ≤ SR ≤ 1.5 indicates additive effect, and SR > 1.5 indicates synergistic effect.

[0139] Experimental Results and Analysis:

[0140] The results of indoor toxicity tests on azoxystrobin, benomyl, and different ratios of the two against cucumber powdery mildew are shown in Table 1. The toxicity regression equations, correlation coefficients r, and EC50 values ​​for each agent are also presented. 50 Value, Theoretical EC 50 The values ​​and the efficiency ratio (SR) are shown in Table 1.

[0141] Table 1. Results of the combined effects of azoxystrobin and benomyl on cucumber powdery mildew.

[0142]

[0143] Table 1 shows the EC50 of azoxystrobin and benomyl against cucumber powdery mildew. 50The values ​​were 1.5234 mg / L and 1.6153 mg / L, respectively. The synergistic ratios of oxadiazon and benomyl at ratios of 3:1, 2:1, 1:1, 1:2, and 1:3 were 1.2889, 1.6012, 2.0686, 1.7708, and 1.5056, respectively, indicating that these ratios showed a certain degree of synergistic effect against cucumber powdery mildew. The SR value was relatively high at a ratio of 1:1; however, its field efficacy requires further verification through field trials.

[0144] Table 2. Raw data of combined toxicity assay of azoxystrobin and benomyl against cucumber powdery mildew.

[0145]

[0146]

[0147] Field efficacy trials

[0148] Example 20: Field trial for the control of powdery mildew in cucumber.

[0149] Test locations: Shouguang City, Shandong Province, and Sanya City, Hainan Province.

[0150] Test reagents: 20% azoxystrobin·benzoxystrobin EC, 20% azoxystrobin·benzoxystrobin SC, 20% azoxystrobin·benzoxystrobin microemulsion, 30% azoxystrobin·benzoxystrobin SC, 30% azoxystrobin·benzoxystrobin EC, 25% azoxystrobin SC, 42% benomyl SC, and water control.

[0151] Experimental target: Cucumber powdery mildew (Erysiphe cichoracearum).

[0152] Application time and frequency: Shouguang was treated once on June 22, 2018; Sanya was treated for the first time on March 26, 2019, and then treated a second time 7 days later.

[0153] The experimental reagents and dosages are detailed in Tables 3 and 4. A blank control was also included. Each treatment was replicated four times, with each plot measuring 20 mg / dL. 2 The cells were randomly arranged. A conventional spraying method was used to evenly spray the solution on both sides of the leaves.

[0154] Investigation and statistical methods: The baseline disease incidence was investigated before pesticide application, and the survey was conducted 7 days after the last pesticide application. Four points were randomly selected from each plot, and all leaves of 1-5 plants were investigated at each point. Each leaf was graded according to the percentage of leaf area covered by disease spots.

[0155] Grading method:

[0156] Grade 0: No lesions;

[0157] Grade 1: The lesion area accounts for less than 5% of the total leaf area;

[0158] Grade 3: The lesion area accounts for 6% to 10% of the total leaf area;

[0159] Level 5: The lesion area accounts for 11% to 20% of the total leaf area;

[0160] Level 7: Lesions cover 21% to 40% of the total leaf area;

[0161] Level 9: The lesion area accounts for more than 40% of the total leaf area.

[0162] Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)] × 100

[0163] Control efficacy (%) = [1 - (disease index before treatment × disease index after treatment) / (disease index after treatment × disease index before treatment)] × 100

[0164] Table 3. Results of the experiment on the control of powdery mildew in cucumber using a mixture of azoxystrobin and benomyl (Shouguang)

[0165]

[0166] Note: Test location: Shouguang City, Shandong Province; first application date: June 22, 2018; one plant was surveyed at each site.

[0167] Table 4. Results of the experiment on the control of cucumber powdery mildew by mixing azoxystrobin and benomyl (Sanya)

[0168]

[0169]

[0170] Note: Test location: Sanya City, Hainan Province; first application date: March 26, 2019; second application 7 days later; 10 plants were surveyed at each site.

[0171] Field efficacy results (see Tables 3 and 4) showed that 20% azoxystrobin·benzoxystrobin EC, 20% azoxystrobin·benzoxystrobin SC, 20% azoxystrobin·benzoxystrobin Microemulsion, 30% azoxystrobin·benzoxystrobin SC, and 30% azoxystrobin·benzoxystrobin EC all had a control efficacy of over 80% against cucumber powdery mildew, which was significantly higher than that of single agents.

[0172] A safety survey of cucumbers showed that no phytotoxicity occurred in cucumbers after application of the pesticides.

[0173] Table 5. Raw data from the experiment on the control of cucumber powdery mildew by mixing azoxystrobin and benomyl (Shouguang)

[0174]

[0175]

[0176] Table 6. Raw data from the experiment on the control of cucumber powdery mildew by mixing azoxystrobin and benomyl (Sanya)

[0177]

[0178]

[0179]

[0180] Example 21: Experiment on the control of strawberry powdery mildew by mixing azoxystrobin and benomyl.

[0181] Test location: Yifengdian Test Base, Jimo City, Qingdao, Shandong Province.

[0182] Test reagents: 20% azoxystrobin·benzoxystrobin EC, 20% azoxystrobin·benzoxystrobin SC, 20% azoxystrobin·benzoxystrobin microemulsion, 30% azoxystrobin·benzoxystrobin SC, 30% azoxystrobin·benzoxystrobin EC, 25% azoxystrobin SC, 42% benomyl SC, and water control.

[0183] Experimental target: strawberry powdery mildew (Sphaerotheca macularis).

[0184] Application time and frequency: The first application was on July 14, 2018.

[0185] The experimental reagents and dosages are detailed in Table 7. A blank control was also included. Each treatment was replicated four times, with each plot containing 20 mg / dL. 2 The cells were randomly arranged. A conventional spraying method was used to evenly spray the solution on both sides of the leaves.

[0186] Survey and statistical methods: The baseline disease incidence was assessed before and 7 days after application. Five points were sampled diagonally across each plot, with one plant sampled at each point, and all leaves of each plant were analyzed. Grading method:

[0187] Grade 0: No lesions;

[0188] Grade 1: The lesion area accounts for less than 5% of the total leaf area;

[0189] Grade 3: Lesions cover 6% to 15% of the total leaf area;

[0190] Level 5: Lesions cover 16% to 25% of the total leaf area;

[0191] Level 7: Lesions cover 26% to 50% of the total leaf area;

[0192] Level 9: The lesion area accounts for more than 51% of the total leaf area;

[0193] Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)] × 100

[0194] Control efficacy (%) = [1 - (disease index before treatment × disease index after treatment) / (disease index after treatment × disease index before treatment)] × 100

[0195] Experimental results:

[0196] Table 7. Control efficacy of a mixture of oxadiazon and benomyl against powdery mildew in strawberries (Sanya)

[0197]

[0198] Field efficacy studies (see Table 7) showed that 20% azoxystrobin·benzoxystrobin EC, 20% azoxystrobin·benzoxystrobin SC, 20% azoxystrobin·benzoxystrobin Microemulsion, 30% azoxystrobin·benzoxystrobin SC, and 30% azoxystrobin·benzoxystrobin EC all had good control effects on strawberry powdery mildew, with control efficacy all above 80%, significantly higher than that of single agents.

[0199] Table 8. Raw data on the control effect of a mixture of oxadiazon and benomyl on strawberry powdery mildew (Sanya)

[0200]

[0201]

[0202]

[0203] Example 22: Control of powdery mildew in zucchini by mixing azoxystrobin and benomyl

[0204] Test location: Ma'an, Huicheng District, Huizhou City, Guangdong Province.

[0205] Test reagents: 20% azoxystrobin·benzoxystrobin EC, 20% azoxystrobin·benzoxystrobin SC, 20% azoxystrobin·benzoxystrobin microemulsion, 30% azoxystrobin·benzoxystrobin SC, 30% azoxystrobin·benzoxystrobin EC, 30% azoxystrobin·benzoxystrobin Aqueous Emulsion, 30% azoxystrobin·benzoxystrobin Aqueous Emulsion, 40% azoxystrobin·benzoxystrobin SC, 40% azoxystrobin·benzoxystrobin Aqueous Emulsion, 25% azoxystrobin SC, 40% benomyl SC, and water control.

[0206] Experimental target: powdery mildew of zucchini.

[0207] The experimental reagents and dosages are detailed in Table 9. A blank control was also included. Each treatment was replicated four times, with each plot containing 20 mg / mL. 2The cells were randomly arranged. A conventional spraying method was used to evenly spray the solution on both sides of the leaves.

[0208] Survey and Statistical Methods: The baseline disease incidence was assessed before pesticide application. Pesticide was applied on November 17, 2018, with a second application 7 days later. The baseline disease incidence was assessed 7 days after the second application. Five points were sampled diagonally across each plot, with one plant sampled at each point, and all leaves of each plant were analyzed. Grading Method:

[0209] Grade 0: No lesions;

[0210] Grade 1: The lesion area accounts for less than 5% of the total leaf area;

[0211] Grade 3: Lesions cover 6% to 15% of the total leaf area;

[0212] Level 5: Lesions cover 16% to 25% of the total leaf area;

[0213] Level 7: Lesions cover 26% to 50% of the total leaf area;

[0214] Level 9: The lesion area accounts for more than 51% of the total leaf area;

[0215] Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)] × 100

[0216] Control efficacy (%) = [1 - (disease index before treatment × disease index after treatment) / (disease index after treatment × disease index before treatment)] × 100

[0217] Experimental results:

[0218] Table 9: Effects of a mixture of azoxystrobin and benomyl on the control of powdery mildew in zucchini (Huizhou)

[0219]

[0220] Field efficacy studies (see Table 9) showed that 20% azoxystrobin·benzoxystrobin EC, 20% azoxystrobin·benzoxystrobin SC, 20% azoxystrobin·benzoxystrobin Microemulsion, 30% azoxystrobin·benzoxystrobin SC, 30% azoxystrobin·benzoxystrobin EC, 30% azoxystrobin·benzoxystrobin Emulsifiable Concentrate, 30% azoxystrobin·benzoxystrobin Emulsifiable Concentrate, 40% azoxystrobin·benzoxystrobin SC, and 40% azoxystrobin·benzoxystrobin Emulsifiable Concentrate all had good control effects on powdery mildew of zucchini, with control efficacy all above 80%, significantly higher than that of single agents.

[0221] Table 10. Raw data on the control effect of a mixture of oxadiazon and benomyl on powdery mildew in zucchini (Huizhou)

[0222]

[0223]

[0224]

[0225] Example 23: Control of powdery mildew in bitter gourd by mixing azoxystrobin and benomyl.

[0226] Test location: Sanya, Hainan.

[0227] Test reagents: 25% azoxystrobin suspension, 40% benomyl suspension, 20% azoxystrobin·benomyl suspension, 20% azoxystrobin·benomyl emulsifiable concentrate, 20% azoxystrobin·benomyl microemulsion, 30% azoxystrobin·benomyl suspension, 30% azoxystrobin·benomyl emulsifiable concentrate, 25% azoxystrobin suspension, 40% benomyl suspension, and water control.

[0228] Experimental target: Powdery mildew of bitter gourd.

[0229] The experimental reagents and dosages are detailed in Table 7. A blank control was also included. Each treatment was replicated four times, with each plot containing 20 mg / dL. 2 The cells were randomly arranged. A conventional spraying method was used to evenly spray the solution on both sides of the leaves.

[0230] Survey and statistical methods: The baseline disease incidence was assessed before pesticide application. Pesticide was applied on March 7, 2019, with a second application 7 days later. The baseline disease incidence was assessed 7 days after the second application. Five points were sampled diagonally across each plot, with 25 branches sampled at each point, and all leaves sampled from each branch. Grading method:

[0231] Grade 0: No lesions;

[0232] Grade 1: The lesion area accounts for less than 5% of the total leaf area;

[0233] Grade 3: Lesions cover 6% to 15% of the total leaf area;

[0234] Level 5: Lesions cover 16% to 25% of the total leaf area;

[0235] Level 7: Lesions cover 26% to 50% of the total leaf area;

[0236] Level 9: The lesion area accounts for more than 51% of the total leaf area;

[0237] Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)] × 100

[0238] Control efficacy (%) = [1 - (disease index before treatment × disease index after treatment) / (disease index after treatment × disease index before treatment)] × 100

[0239] Experimental results:

[0240] Table 11. Effects of a mixture of azoxystrobin and benomyl on the control of powdery mildew in bitter gourd (Sanya)

[0241]

[0242] Field efficacy studies (see Table 11) showed that 5% azoxystrobin suspension, 40% benomyl suspension, 20% azoxystrobin·benomyl suspension, 20% azoxystrobin·benomyl emulsifiable concentrate, 20% azoxystrobin·benomyl microemulsion, 30% azoxystrobin·benomyl suspension, and 30% azoxystrobin·benomyl emulsifiable concentrate all had good control effects on powdery mildew of bitter gourd, with control efficacy all above 80%, significantly higher than that of single agents.

[0243] Table 12. Raw data on the efficacy of a mixture of azoxystrobin and benomyl in controlling powdery mildew of bitter gourd (Sanya)

[0244]

[0245]

Claims

1. The use of a fungicide composition containing oxime ester for the prevention and control of crop diseases, characterized in that, The active ingredients of the bactericidal composition are oxadiazon and benomyl, with a weight ratio of oxadiazon to benomyl of 1:3 to 2:

1. The crop is cucumber and / or strawberry, and the crop disease is cucumber powdery mildew and / or strawberry powdery mildew.

2. The use according to claim 1, characterized in that, The total weight of oxadiazon and benomyl accounts for 1% to 90% of the composition.

3. The use according to claim 1, characterized in that, The total weight of oxadiazon and benomyl accounts for 10% to 60% of the composition.

4. The use according to claim 1, characterized in that, This bactericidal composition can be formulated into any formulation suitable for agricultural use.

5. The use according to claim 4, characterized in that, The bactericidal composition can be prepared into an agriculturally permissible formulation, wherein the formulation is selected from wettable powder, water-dispersible granules, suspension concentrate, microemulsion, emulsifiable concentrate or water emulsion.

6. A method for preventing and controlling crop diseases, characterized in that, The fungicidal composition as described in any one of claims 1-5 is applied to the growing area and / or leaves and / or stems of the crop.

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