Human interferon α1b and preparation method thereof

By expressing human interferon α1b in the periplasmic cells of E. coli and optimizing the fermentation and culture conditions, the problem of low purification efficiency in the prior art was solved, and the preparation of human interferon α1b with high efficiency, high purity and high biological activity was achieved.

CN113930468BActive Publication Date: 2025-04-04SHENZHEN KEXING PHARM CO LTD
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Patent Information

Application Number
CN202111344359.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2021-11-12
Publication Date
2025-04-04
Estimated Expiration
2041-11-12

AI Technical Summary

Technical Problem

In the preparation process of recombinant human interferon α1b in the prior art, E. coli cells need to be broken down, resulting in the release of host proteins, increasing purification steps, and reducing purification efficiency.

Method used

E. coli, which can express human interferon α1b in periplasmic cells, is used to improve the expression amount and biological activity of human interferon α1b by optimizing the fermentation medium components, inducer addition timing and culture temperature.

Benefits of technology

The purification process is simplified, the purity and biological activity of human interferon α1b are improved, and the preparation cost is reduced.

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Abstract

The present invention relates to a recombinant human interferon α1b and a preparation method thereof, and belongs to the field of biomedicine. The method is simple to operate, can make Escherichia coli express human interferon α1b in the periplasmic cell space, can capture it without breaking the cells, reduces host protein release, simplifies the purification steps, and the obtained human interferon α1b has high purity and high activity, which is conducive to the industrial production of human interferon α1b.
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Citation Information

Patent Citations

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