A biological cell activity detection device

Abstract

This invention belongs to the field of biological detection technology, specifically relating to a biological cell activity detection device, including an upper chamber and a lower chamber. The upper chamber is equipped with a test tube placement mechanism, and the lower chamber is equipped with a housing and a heating mechanism. The test tube placement mechanism includes a panel, an inner test tube rack, and an outer test tube rack. The panel has an installation groove, and the inner and outer test tube racks are connected by a sliding cylinder. The installation groove is equipped with a connector, and the connector is connected to a sliding rod. The connector is equipped with a vertical connecting rod, and the vertical connecting rod is connected to a ring platform. The ring platform is equipped with a push rod. The upper chamber is connected to a rotating ring platform. The heating mechanism includes an electric heater, an electric heating grid, and an exhaust fan. This invention enables the test sample tubes inserted into the inner test tube rack and the test sample tubes inserted into the outer test tube rack to maintain the same oscillation amplitude, avoiding the problem of large differences in the test results caused by inconsistent amplitudes of the test sample tubes.

Description

Technical Field

[0001] This invention belongs to the field of biological detection technology, and specifically relates to a biological cell activity detection device. Background Technology

[0002] Cell viability refers to the proportion of healthy cells in a sample population. Detecting cell viability is essential for observing the physiological state of cells during experiments. Among current cell viability detection methods, the MTT assay is an earlier and more classic method. The MTT cell viability detection method mainly uses a reaction reagent to react with a cell solution, and then measures the absorbance of the solution to a specific wavelength of light to determine cell viability.

[0003] An existing utility model patent (application number: CN202120747429.2) discloses a stem cell activity detection kit, including a box body. The bottom of the box body is provided with a removable base plate. The base plate is provided with a rotatable turntable. The turntable is provided with a circular track with varying heights. A tray is provided horizontally above the turntable. The bottom surface of the tray is provided with several spring sleeves. Springs are provided inside the spring sleeves. Wheel plates are provided at the bottom ends of the springs. Rollers for rolling in the track are provided below the wheel plates. A universal joint is provided between the turntable and the tray.

[0004] In the above-mentioned utility model, the test sample tube inserted into the test tube sleeve can be oscillated. However, during the oscillation of the test sample tube, the oscillation amplitude of the test sample tube near the center of the tray is small, while the oscillation amplitude of the test sample tube near the edge of the tray is large. Therefore, the oscillation amplitude of each test sample tube is not the same, which makes the test results of each test sample tube vary greatly. Summary of the Invention

[0005] The purpose of this invention is to provide a biological cell activity detection device to solve the problems mentioned in the background art.

[0006] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0007] A biological cell activity detection device includes an upper chamber and a lower chamber, which are fixedly connected and interconnected. The upper chamber is provided with a test tube placement mechanism, and the lower chamber is provided with a housing and a heating mechanism.

[0008] The test tube placement mechanism includes a panel, several inner test tube racks, and several outer test tube racks. The panel is fixedly mounted on the upper side of the upper housing. The panel has several mounting slots arranged in a circumferential array. The mounting slots extend radially along the panel. The number of inner test tube racks, outer test tube racks, and mounting slots are equal and correspond one-to-one. The inner test tube racks are located on the side of the mounting slots closer to the center of the panel, and the outer test tube racks are located on the side of the mounting slots closer to the edge of the panel.

[0009] The inner test tube rack is hinged to the mounting groove near the center of the panel, and the outer test tube rack is hinged to the mounting groove near the edge of the panel. Sliding cylinders are fixedly connected to the facing sides of both the inner and outer test tube racks. A connector is provided in the middle of the mounting groove, and sliding rods are fixedly connected to both sides of the connector. The sliding rods on both sides are slidably fitted with the two sliding cylinders respectively. A vertical connecting rod is provided at the bottom of the connector, and a ring platform is fixedly connected to the vertical connecting rod. Push rods are symmetrically arranged at the bottom of the ring platform. A rotating ring platform is also rotatably connected to the lower side of the upper housing. The rotating ring platform has two symmetrically arranged protrusions and two symmetrically arranged concave parts, with a smooth transition between the protrusions and concave parts. The push rods abut against the rotating ring platform, and the housing is connected to the rotating ring platform via a transmission mechanism.

[0010] The heating mechanism includes an electric heater, an electric heating grid, and an exhaust fan. The electric heater is electrically connected to the electric heating grid, and the exhaust fan is located above the electric heating grid.

[0011] The lower inner wall of the upper housing is provided with an annular slide rail, and the side of the rotating ring platform is provided with several connecting rods, each of which extends into the annular slide rail.

[0012] The output shaft of the chassis extends through the lower housing and is fixedly connected to a first helical tooth. A first mounting platform is fixedly provided on the lower side of the upper housing. A first rotating rod is rotatably connected to the bottom of the first mounting platform. A transmission gear ring is fixedly connected to the first rotating rod. A reduction gear ring is fixedly connected to the lower end of the rotating ring platform. The transmission gear ring meshes with the reduction gear ring. A second helical tooth that meshes with the first helical tooth is also fixedly connected to the end of the first rotating rod.

[0013] The tooth ratio between the reduction gear ring and the transmission gear ring is 8:1.

[0014] The lower housing is also fixedly equipped with a frame, which is fixedly connected to a second mounting platform. The second mounting platform is rotatably connected to a second rotating rod, the lower end of which is fixedly connected to the exhaust fan. A transmission mechanism is also provided between the second rotating rod and the first rotating rod.

[0015] The transmission mechanism includes two identical transmission pulleys disposed in the lower housing. One of the transmission pulleys is fixedly connected to the first rotating rod, and the other transmission pulley is fixedly connected to the second rotating rod. The two transmission pulleys are connected by a belt drive.

[0016] A screen is also installed at the bottom of the lower box.

[0017] A mounting base is also provided below the lower housing, and several support rods are fixedly connected to the mounting base. The support rods are fixedly connected to the lower housing.

[0018] When using this device, first seal the test sample tube, then insert it into the inner or outer test tube rack. Turn on the heater and exhaust fan, with the exhaust fan blowing air inwards. The heating element heats the inside of the device, maintaining a suitable temperature. Then, start the machine, which drives the rotating ring platform to rotate. The push rod abuts against the protrusions and recesses of the rotating ring platform, causing the push rod, ring platform, and vertical connecting rod to move up and down reciprocally. This causes the test sample tubes inserted into the inner and outer test tube racks to oscillate synchronously with equal amplitude, thus avoiding inconsistent oscillation amplitudes that could lead to significant differences in the test results. Attached Figure Description

[0019] The present invention can be further illustrated by the non-limiting embodiments given in the accompanying drawings;

[0020] Figure 1 This is a schematic diagram of an embodiment of a biological cell activity detection device according to the present invention;

[0021] Figure 2 This is a schematic diagram of the internal cross-sectional structure of an embodiment of a biological cell activity detection device of the present invention;

[0022] Figure 3 for Figure 2 Enlarged schematic diagram of the structure at point A;

[0023] Figure 4 for Figure 2 Enlarged schematic diagram of the structure at point B;

[0024] Figure 5 This is a schematic diagram of the test tube placement mechanism according to an embodiment of the present invention;

[0025] Figure 6 This is a schematic diagram of the rotating ring platform according to an embodiment of the present invention. Figure 1 ;

[0026] Figure 7 This is a schematic diagram of the rotating ring platform according to an embodiment of the present invention. Figure 2 ;

[0027] Figure 8 This is a schematic diagram of the panel and mounting groove according to an embodiment of the present invention;

[0028] The symbols for the main components are explained below:

[0029] Upper housing 1, rotating ring platform 11, protrusion 111, recess 112, connecting rod 113, reduction gear ring 114, annular slide rail 12, first mounting platform 13, first rotating rod 14, transmission gear ring 141, second helical gear 142, lower housing 2, chassis 21, first helical gear 22, panel 31, mounting groove 311, inner test tube rack 32, outer test tube rack 33, slide cylinder 34, connector 35, slide rod 351, vertical connecting rod 36, ring platform 361, push rod 362, electric heater 41, electric heating mesh 42, exhaust fan 43, frame 51, second mounting platform 52, second rotating rod 53, transmission pulley 61, screen 71, mounting base 72, support rod 73. Detailed Implementation

[0030] To enable those skilled in the art to better understand the present invention, the technical solution of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0031] Example 1:

[0032] like Figures 1-8 As shown, a biological cell activity detection device of the present invention includes an upper box 1 and a lower box 2, which are fixedly connected and interconnected. The upper box 1 is provided with a test tube placement mechanism, and the lower box 2 is provided with a box 21 and a heating mechanism.

[0033] The test tube placement mechanism includes a panel 31, several inner test tube racks 32 and several outer test tube racks 33. The panel 31 is fixedly installed on the upper side of the upper box 1. The panel 31 has several mounting slots 311 arranged in a circular array. The mounting slots 311 extend radially along the panel 31. The number of inner test tube racks 32, outer test tube racks 33 and mounting slots 311 are equal and correspond one-to-one. The inner test tube racks 32 are located on the side of the mounting slots 311 near the center of the panel 31, and the outer test tube racks 33 are located on the side of the mounting slots 311 near the edge of the panel 31.

[0034] The inner test tube rack 32 is hinged to the mounting groove 311 on the side near the center of the panel 31, and the outer test tube rack 33 is hinged to the mounting groove 311 on the side near the edge of the panel 31. Sliding cylinders 34 are fixedly connected to the facing sides of both the inner and outer test tube racks 32 and 33. A connector 35 is provided in the middle of the mounting groove 311, and sliding rods 351 are fixedly connected to both sides of the connector 35. The sliding rods 351 on both sides are slidably fitted with the two sliding cylinders 34 respectively. A bottom part of the connector 35 is provided with… There is a vertical connecting rod 36, which is fixedly connected to a ring platform 361. Push rods 362 are symmetrically arranged at the bottom of the ring platform 361. A rotating ring platform 11 is also rotatably connected to the lower side of the upper housing 1. The rotating ring platform 11 is machined with two symmetrically arranged protrusions 111 and two symmetrically arranged concave parts 112. The protrusions 111 and the concave parts 112 are smoothly transitioned. The push rods 362 abut against the rotating ring platform 11. The housing 21 is connected to the rotating ring platform 11 through a transmission.

[0035] The heating mechanism includes an electric heater 41, an electric heating grid 42, and an exhaust fan 43. The electric heater 41 is electrically connected to the electric heating grid 42, and the exhaust fan 43 is located above the electric heating grid 42.

[0036] The mounting slot 311 is used on both sides to mount the inner test tube rack 32 and the outer test tube rack 33. During biological cell activity detection, the test sample tube is placed in the inner test tube rack 32 and the outer test tube rack 33. The inner test tube rack 32 is hinged to the mounting slot 311 on the side near the center of the panel 31, and the outer test tube rack 33 is hinged to the mounting slot 311 on the side near the edge of the panel 31. Therefore, both the inner test tube rack 32 and the outer test tube rack 33 can be rotated in the mounting slot 311. When the chassis 21 is started, the rotating ring platform 11 can rotate. During the rotation, the protrusion 111 and the concave part 112 of the rotating ring platform 11 can continuously abut against the push rod 362, so that the ring platform 361 and the vertical connecting rod 36 move along... The protrusion 111 and the recess 112 move up and down repeatedly, causing the connector 35 to move up and down. During this process, when the push rod 362 moves to the highest point of the protrusion 111, the connector 35 rises to its highest point. The slide rods 351 on both sides of the connector 35 extend upward from the two slide cylinders 34, causing the lower ends of the inner test tube rack 32 and the outer test tube rack 33 to swing closer together. This causes the test sample tubes in the inner test tube rack 32 and the outer test tube rack 33 to flip and oscillate, bringing them closer together. When the push rod 362 moves to the middle position between the protrusion 111 and the recess 112, the connector 35 is in the mounting groove 311, and the slide rods 351 on both sides of the connector 35 are fully retracted into the two slide cylinders 34. In cylinder 34, the lower end of the inner test tube rack 32 and the outer test tube rack 33 are in a vertical state, causing the test sample tubes in the inner test tube rack 32 and the test sample tubes in the outer test tube rack 33 to rotate and oscillate in opposite directions until they are parallel. As the push rod 362 moves to the lowest point of the recess 112, the connector 35 descends to the lowest point. The slide rods 351 on both sides of the connector 35 extend downward from the two slide cylinders 34, causing the lower ends of the inner test tube rack 32 and the outer test tube rack 33 to swing away from each other. This causes the test sample tubes in the inner test tube rack 32 and the test sample tubes in the outer test tube rack 33 to rotate and oscillate in opposite directions and move away from each other. Through the continuous rotation of the rotating ring 11, the test sample tubes in the inner test tube rack 32 are further rotated away from each other. The test sample tubes in the outer test tube rack 33 can reciprocate and oscillate. Since the connector 35 is located in the middle of the mounting groove 311, the oscillation amplitude of the inner test tube rack 32 and the outer test tube rack 33 is always consistent. This ensures that the oscillation amplitude of the test sample tubes in the inner test tube rack 32 and the outer test tube rack 33 is always consistent. Furthermore, regardless of how the test sample tubes are placed in the inner test tube rack 32 or the outer test tube rack 33, the oscillation amplitude of all test sample tubes remains consistent. The exhaust fan 43 is used for ventilation. The electric heater 41 heats the electric heating grid 42, which heats the drawn-in air, thereby maintaining the internal temperature of the device at a suitable level, which is more conducive to the detection of cell activity.

[0037] When using the device, first seal the test sample tube, then insert it into the inner test tube rack 32 or the outer test tube rack 33. Start the electric heater 41 and the exhaust fan 43. The exhaust fan 43 blows air inward and heats the device through the electric heating grid 42, keeping the inside of the device at a suitable temperature. Then, start the machine box 21 to drive the rotating ring platform 11 to rotate. Through the contact between the push rod 362 and the protrusion 111 and the concave part 112 of the rotating ring platform 11, the push rod 362, the ring platform 361 and the vertical connecting rod 36 reciprocate up and down, thereby causing the test sample tubes inserted into the inner test tube rack 32 and the outer test tube rack 33 to oscillate synchronously with equal amplitude.

[0038] The present invention enables the test sample tubes inserted into the inner test tube rack 32 and the test sample tubes inserted into the outer test tube rack 33 to maintain the same oscillation amplitude, thereby avoiding the problem of inconsistent oscillation amplitudes of the test sample tubes during oscillation, which leads to large differences in the test results of the test sample tubes.

[0039] Please see Figure 2 The lower inner wall of the upper housing 1 is provided with an annular slide rail 12, and the side of the rotating ring platform 11 is provided with several connecting rods 113, each of which extends into the annular slide rail 12.

[0040] By setting the annular slide rail 12 and connecting rod 113, the rotating ring platform 11 can be stably connected to the upper box 1, which has a simple structure and is easy to process.

[0041] Please see Figure 3 The output shaft of the chassis 21 extends through the lower housing 2 and is fixedly connected to the first helical gear 22. The lower side of the upper housing 1 is fixedly provided with a first mounting platform 13. The bottom of the first mounting platform 13 is rotatably connected to a first rotating rod 14. The first rotating rod 14 is fixedly connected to a transmission gear ring 141. The lower end of the rotating ring platform 11 is fixedly connected to a reduction gear ring 114. The transmission gear ring 141 and the reduction gear ring 114 mesh and match. The gear ratio between the reduction gear ring 114 and the transmission gear ring 141 is 8:1. The end of the first rotating rod 14 is also fixedly connected to a second helical gear 142 that meshes and matches the first helical gear 22.

[0042] When the chassis 21 is started, it drives the first helical gear 22 to rotate. Since the second helical gear 142 meshes with the first helical gear 22, the second helical gear 142 rotates, which drives the first rotating rod 14 to rotate relative to the first mounting platform 13, thereby causing the transmission gear ring 141 to rotate. The transmission gear ring 141 drives the reduction gear ring 114 to rotate at low speed, which in turn causes the rotating ring platform 11 to rotate at low speed, causing the test tubes in the inner test tube rack 32 and the outer test tube rack 33 to oscillate at low speed.

[0043] Example 2:

[0044] Based on Example 1, the following improvements are made:

[0045] Please see Figure 2 and Figure 4 Inside the lower housing 2, a frame 51 is fixedly installed. The frame 51 is fixedly connected to a second mounting platform 52. The second mounting platform 52 is rotatably connected to a second rotating rod 53. The lower end of the second rotating rod 53 is fixedly connected to an exhaust fan 43. A transmission mechanism is also provided between the second rotating rod 53 and the first rotating rod 14. The transmission mechanism includes two identical transmission pulleys 61 installed in the lower housing 2. One transmission pulley 61 is fixedly connected to the first rotating rod 14, and the other transmission pulley 61 is fixedly connected to the second rotating rod 53. The two transmission pulleys 61 are connected by a belt drive.

[0046] In this embodiment, the frame 51 and the second mounting platform 53 are used to mount the second rotating rod 53. Through the transmission mechanism, when the first rotating rod 14 rotates, the second rotating rod 53 can rotate through the belt drive between the two transmission pulleys 61. The transmission structure is simple, and the two transmission pulleys 61 are of the same specification, so that the rotation speed between the first rotating rod 14 and the second rotating rod 53 is the same, which will not cause the exhaust fan 43 to rotate at a low speed, and the exhaust fan 43 can be rotated without the need to install other motors.

[0047] Example 3:

[0048] Based on Example 2, the following improvements are made:

[0049] Please see Figure 1 , Figure 2 and Figure 8 The bottom of the lower box 2 is also equipped with a screen 71, and a mounting base 72 is also provided below the lower box 2. Several support rods 73 are fixedly connected to the mounting base 72, and the support rods 73 are fixedly connected to the lower box 2.

[0050] In this embodiment, when the exhaust fan 43 rotates to exhaust air, the screen 71 can filter impurities in the air, thereby keeping the interior of the upper box 1 and the lower box 2 clean. The mounting base 72 and support rod 73 can provide stable support for the entire device, making the device more stable when placed.

[0051] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A biological cell activity detection device, comprising an upper chamber and a lower chamber, wherein the upper chamber and the lower chamber are fixedly connected and interconnected, characterized in that: The upper chamber is equipped with a test tube placement mechanism, and the lower chamber is equipped with a cabinet and a heating mechanism. The test tube placement mechanism includes a panel, several inner test tube racks, and several outer test tube racks. The panel is fixedly mounted on the upper side of the upper housing. The panel has several mounting slots arranged in a circumferential array. The mounting slots extend radially along the panel. The number of inner test tube racks, outer test tube racks, and mounting slots are equal and correspond one-to-one. The inner test tube racks are located on the side of the mounting slots closer to the center of the panel, and the outer test tube racks are located on the side of the mounting slots closer to the edge of the panel. The inner test tube rack is hinged to the mounting groove near the center of the panel, and the outer test tube rack is hinged to the mounting groove near the edge of the panel. Sliding cylinders are fixedly connected to the facing sides of both the inner and outer test tube racks. A connector is provided in the middle of the mounting groove, and sliding rods are fixedly connected to both sides of the connector. The sliding rods on both sides are slidably fitted with the two sliding cylinders respectively. A vertical connecting rod is provided at the bottom of the connector, and a ring platform is fixedly connected to the vertical connecting rod. Push rods are symmetrically arranged at the bottom of the ring platform. A rotating ring platform is also rotatably connected to the lower side of the upper housing. The rotating ring platform has two symmetrically arranged protrusions and two symmetrically arranged concave parts, with a smooth transition between the protrusions and concave parts. The push rods abut against the rotating ring platform, and the housing is connected to the rotating ring platform via a transmission mechanism. The heating mechanism includes an electric heater, an electric heating grid, and an exhaust fan. The electric heater is electrically connected to the electric heating grid, and the exhaust fan is located above the electric heating grid.

2. The biological cell activity detection device according to claim 1, characterized in that: The lower inner wall of the upper housing is provided with an annular slide rail, and the side of the rotating ring platform is provided with several connecting rods, each of which extends into the annular slide rail.

3. The biological cell activity detection device according to claim 1, characterized in that: The output shaft of the chassis extends through the lower housing and is fixedly connected to a first helical tooth. A first mounting platform is fixedly provided on the lower side of the upper housing. A first rotating rod is rotatably connected to the bottom of the first mounting platform. A transmission gear ring is fixedly connected to the first rotating rod. A reduction gear ring is fixedly connected to the lower end of the rotating ring platform. The transmission gear ring meshes with the reduction gear ring. A second helical tooth that meshes with the first helical tooth is also fixedly connected to the end of the first rotating rod.

4. The biological cell activity detection device according to claim 3, characterized in that: The tooth ratio between the reduction gear ring and the transmission gear ring is 8:

1.

5. The biological cell activity detection device according to claim 4, characterized in that: The lower housing is also fixedly equipped with a frame, which is fixedly connected to a second mounting platform. The second mounting platform is rotatably connected to a second rotating rod, the lower end of which is fixedly connected to the exhaust fan. A transmission mechanism is also provided between the second rotating rod and the first rotating rod.

6. The biological cell activity detection device according to claim 5, characterized in that: The transmission mechanism includes two identical transmission pulleys disposed in the lower housing. One of the transmission pulleys is fixedly connected to the first rotating rod, and the other transmission pulley is fixedly connected to the second rotating rod. The two transmission pulleys are connected by a belt drive.

7. The biological cell activity detection device according to claim 6, characterized in that: A screen is also installed at the bottom of the lower box.

8. The biological cell activity detection device according to claim 7, characterized in that: A mounting base is also provided below the lower housing, and several support rods are fixedly connected to the mounting base. The support rods are fixedly connected to the lower housing.

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