Artificial feed for low-age larvae of the moth bean caterpillar

Abstract

The application provides an artificial feed for low-age larvae of the moth Dendrolimus punctatus Walker, and specifically, the artificial feed comprises: (1) a dispersion system, wherein the dispersion system comprises agar and pure water; (2) a sugar compound, wherein the sugar compound comprises components selected from the group consisting of wheat germ powder, bean leaf powder and salt; and (3) a protein compound, wherein the protein compound comprises components selected from the group consisting of sucrose, casein and egg yolk powder. The feed formula can make newly hatched larvae actively orient and feed on the feed, and can ensure that the larvae molt into the 2nd instar after feeding for 3-5 days and molt into the 3rd instar after feeding for 7-10 days.

Description

Technical Field

[0001] The present invention relates to the field of agricultural breeding, and specifically, the present invention provides an artificial feed formula for young (1st to 2nd instar) larvae of Clanis bilineata Walker. Background Art

[0002] Clanis bilineata Walker, belonging to the genus Clanis, family Sphingidae, order Lepidoptera, class Insecta, is a holometabolous insect. Its entire life cycle includes four insect stages: egg, larva, pupa and adult. The larva has a total of 5 instars, and the mature 5th-instar larva burrows into soil for overwintering. Because its larvae are rich in nutritional value and have unique flavor, they are called "Doudan". There is a habit of eating Doudan in many regions of China, especially Guanyun County, Jiangsu Province, which is famous for producing and consuming Doudan. "Guanyun Doudan" has become a famous local snack and regional business card, and is an essential dish for locals to entertain distinguished guests.

[0003] In recent years, as a distribution base for the breeding, acquisition and sales of Doudan, Guanyun has an annual transaction volume of more than 10,000 tons and an annual output value of hundreds of millions of RMB. It has not only solved the employment problem of some surplus labor forces, but also activated the local economy. The main host plants of Clanis bilineata Walker larvae are soybean (Glycine max), mung bean (Vigna radiata) and cowpea (Vigna unguiculata), and they also feed on the leaves of black locust (Robinia pseudoacacia) and Pueraria omeiensis. Edible Doudan is mostly cultured in field covered nets by planting soybeans, and its production capacity is relatively low. Therefore, if an artificial feed for breeding Doudan can be developed, it can not only produce more Doudan in limited space, improve production efficiency, and solve the employment problem, but also realize year-round supply of Doudan to meet the increasing market demand.

[0004] In summary, there is an urgent need in the art to develop a feed composition suitable for Doudan breeding. Summary of the Invention

[0005] The object of the present invention is to provide a feed composition suitable for Doudan breeding.

[0006] In a first aspect of the present invention, there is provided an artificial feed for young larvae of Clanis bilineata Walker, said artificial feed comprising:

[0007] (1) Dispersion system: said dispersion system comprises agar and pure water;

[0008] (2) Carbohydrate complex: said carbohydrate complex comprises components selected from the group consisting of wheat germ powder, soybean leaf powder, corn flour and table salt;

[0009] (3) Protein complex: The protein complex includes components selected from the group consisting of sucrose, glucose, casein and egg yolk powder.

[0010] In another preferred embodiment, the artificial feed further includes: compound vitamins and / or sorbic acid.

[0011] In another preferred embodiment, the mass ratio of carbohydrate complex to protein complex in the artificial feed is 8-10:7-8.

[0012] In another preferred embodiment, the carbohydrate complex comprises wheat germ powder, soybean leaf powder, and salt; preferably, the mass ratio of the wheat germ powder, soybean leaf powder, and salt is 2-4:4-6:0.8-1.2.

[0013] In another preferred embodiment, the protein complex comprises sucrose, casein, and egg yolk powder; preferably, the mass ratio of sucrose, casein, and egg yolk powder is 12-16:12-16:0.8-1.2.

[0014] In another preferred embodiment, in the artificial feed, the mass ratio of the dispersion system to the total mass of the carbohydrate complex and the protein complex is m(dispersion system):m(carbohydrate complex + protein complex) = 1-3:8-12.

[0015] In another preferred embodiment, the artificial feed comprises:

[0016] Nutrient agar 15-20 pure water 800-850 wheat germ flour 20-40 Bean leaf powder 40-60 Salt 8-12 sucrose 30-40 casein 30-40 Egg yolk powder 2-3 Multivitamin solution 8-12 Sorbic acid 0.2-0.8

[0017] .

[0018] A second aspect of the present invention provides a method for preparing artificial feed for young larvae of the soybean hawk moth as described in the first aspect of the present invention, the method comprising the steps of: dispersing a carbohydrate complex and a protein complex separately in the dispersion system to obtain the artificial feed.

[0019] In another preferred embodiment, the method further includes adding a complex vitamin solution and / or p-hydroxybenzoic acid to the dispersion system.

[0020] In another preferred embodiment, the method includes:

[0021] Dissolve the agar in pure water at 40-50℃ to obtain the first dispersion;

[0022] The carbohydrate complex and the protein complex were dissolved in pure water to obtain the first solution;

[0023] At 90-100℃, the first dispersion is added to the first solution to form a second dispersion;

[0024] Water or ice is added to the second dispersion to cool it to 40-50°C, and then a compound vitamin solution and / or p-hydroxybenzoic acid are added to obtain a third dispersion.

[0025] The third dispersion is cooled and solidified to obtain the artificial feed.

[0026] In another preferred embodiment, the first solution is prepared under heating conditions.

[0027] In another preferred embodiment, the carbohydrate complex includes wheat germ powder, which is pre-baked at 80-120°C.

[0028] In another preferred embodiment, the method further includes: after the artificial feed has cooled and solidified, cutting it into an appropriate shape for feeding.

[0029] A third aspect of the present invention provides a method for raising young larvae of the soybean hawk moth, the method comprising: feeding young larvae of the soybean hawk moth using the feed described in the first aspect of the present invention.

[0030] It should be understood that, within the scope of this invention, the above-described technical features of this invention and the technical features specifically described below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be described in detail here. Attached Figure Description

[0031] Figure 1 Prepared feed for 1-2 year old soybean cubs;

[0032] Figure 2 The cutting method and shape of the feed;

[0033] Figure 3 This describes the feeding and growth status of the bean weevil in its feed, among which... Figure 3 A represents the feeding status of a newly hatched, 1-year-old bean weevil. Figure 3 B represents the feeding state of the bean weevil after 2-3 days, when its color changes from pale yellow to pale green.

[0034] Figure 4 To feed on artificial feed ( Figure 4 A) and peeling ( Figure 4 B) 2-year-old beetle.

[0035] Figure 5 For comparison of 1st to 3rd instar larvae cultured on artificial feed, 5A represents 1st instar larvae; 5B represents 2nd instar larvae; and 5C represents 3rd instar larvae. Detailed Implementation

[0036] Through long-term and in-depth research, the inventors have developed an artificial feed formula for bean weevils. This formula enables newly hatched larvae to actively orient themselves and feed on the feed, and ensures that they molt and enter the second instar 3-5 days after feeding. Based on the above findings, the inventors have completed this invention.

[0037] the term

[0038] As used in this article, the term "bean hawk moth" (Clanis bilineata Walker) refers to the genus Clanis in the family Sphingidae of the order Lepidoptera. It is a holometabolous insect, with its entire life cycle consisting of four stages: egg, larva, pupa, and adult. The larvae have five instars. In this article, the terms "bean hawk moth larva" and "bean moth" are used interchangeably, both referring to the bean hawk moth in its larval stage.

[0039] As used in this article, the term "young bean hawk moth" refers to 1st-2nd instar bean hawk moth larvae.

[0040] Artificial feed for young larvae of the soybean hawk moth

[0041] This invention provides an artificial feed for early-stage (1st to 2nd instar) larvae of the soybean hawk moth, the artificial feed comprising:

[0042] (1) Dispersion system: The dispersion system includes agar and pure water;

[0043] (2) Carbohydrate complex: The carbohydrate complex includes components selected from the group consisting of wheat germ powder, soybean leaf powder and salt;

[0044] (3) Protein complex: The protein complex includes components selected from the group consisting of sucrose, casein and egg yolk powder.

[0045] The artificial feed of the present invention may also include other nutrients. For example, in a preferred embodiment, the artificial feed further includes: compound vitamins and / or sorbic acid.

[0046] The proportions of the various components can be determined according to actual conditions, but in a preferred embodiment, the mass ratio of the carbohydrate complex to the protein complex is 8-10:7-8. The amount of the dispersion system is determined based on the total mass of the carbohydrate complex and the protein complex. In a preferred embodiment, the mass ratio of the dispersion system to other nutrients is m(dispersion system):m(carbohydrate complex + protein complex) = 1-3:8-12.

[0047] The amount of each component in the aforementioned carbohydrate complex can be determined according to actual needs. In a preferred embodiment, the mass ratio of wheat germ powder, soybean leaf powder, and salt in the carbohydrate complex is 2-4:4-6:0.8-1.2.

[0048] The amount of each component in the protein complex can be determined according to actual needs. In a preferred embodiment, the mass ratio of sucrose, casein, and egg yolk powder in the protein complex is 12-16:12-16:0.8-1.2.

[0049] In a preferred embodiment of the present invention, the artificial feed comprises the following components:

[0050] Nutrient agar 15-20 pure water 800-850 wheat germ flour 20-40 Bean leaf powder 40-60 Salt 8-12 sucrose 30-40 casein 30-40 Egg yolk powder 2-3 Multivitamin solution 8-12 Sorbic acid 0.2-0.8

[0051] .

[0052] Preparation of artificial feed for young larvae of the soybean hawk moth

[0053] The present invention also provides a method for preparing artificial feed for early-stage (1st to 2nd instar) larvae of the soybean hawk moth, the method comprising the steps of: dispersing a carbohydrate complex and a protein complex separately in the dispersion system to obtain the artificial feed, and optionally the step of: adding a compound vitamin solution and / or p-hydroxybenzoic acid to the dispersion system.

[0054] Preferably, the preparation method of the present invention includes the following steps:

[0055] Dissolve the agar in pure water at 40-50℃ to obtain the first dispersion;

[0056] The carbohydrate complex and the protein complex were dissolved in pure water to obtain the first solution;

[0057] At 90-100℃, the first dispersion is added to the first solution to form a second dispersion;

[0058] Water or ice is added to the second dispersion to cool it to 40-50°C, and then a compound vitamin solution and / or p-hydroxybenzoic acid are added to obtain a third dispersion.

[0059] The third dispersion is cooled and solidified to obtain the artificial feed.

[0060] The first solution can be prepared under heating conditions, that is, the first solution is prepared under heating conditions, and then heated to 90-100°C, and the first dispersion is added.

[0061] In a preferred embodiment of the present invention, the carbohydrate complex includes wheat germ powder, and the wheat germ powder is pre-baked at 80-120°C.

[0062] In a preferred embodiment, the method further includes: after the artificial feed has cooled and solidified, cutting it into suitable shapes for feeding. Because the feed of the present invention can be processed into any solid form, it is particularly suitable for grid-based rearing of bean hawk moth larvae.

[0063] Compared with the prior art, the main advantages of the present invention are:

[0064] 1. Using this formula, newly hatched larvae can actively orient themselves and feed on the feed, and can be guaranteed to molt and enter the second instar after 3 to 5 days of feeding. Therefore, it can be used for large-scale, intensive artificial breeding of bean larvae.

[0065] 2. Since all the ingredients of the feed of the present invention are human food, the safety of the edible bean weevils obtained through breeding can be guaranteed.

[0066] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Experimental methods in the following embodiments, unless otherwise specified, are generally performed under conventional conditions or as recommended by the manufacturer. Percentages and parts are by weight unless otherwise stated.

[0067] Example 1: Preparation of artificial feed for young larvae of the soybean hawk moth

[0068] Prepare artificial feed for young larvae of the soybean hawk moth according to the feed formula shown in Table 1.

[0069] Table 1. Artificial feed formula for young larvae of the soybean hawk moth (1000g)

[0070]

[0071]

[0072] After preparing all the raw materials, weigh them according to the quantities in column three, and then prepare the artificial feed for bean weevils according to the order in column one. The specific preparation method is as follows:

[0073] Divide the pure water into three portions: one 300ml portion for dissolving the nutrient agar separately; one 300ml portion for dissolving other ingredients; and one 210ml portion for cooling (or you can use 210g of ice instead). Roast the wheat germ powder at 80-120℃ to develop its aroma. Add the vitamin solution and para-hydroxybenzoic acid at around 45℃ and mix well.

[0074] Configure it by following these steps:

[0075] First, heat 300ml of water to 45℃, add the weighed nutrient agar, continue heating and stirring until the agar is completely dissolved, and set aside.

[0076] The second step is to add wheat germ powder, bean leaf powder and salt to 300ml of water and stir well. Then start heating. Then add sucrose, casein and egg yolk powder in sequence and heat to 95℃ while stirring. Stop heating and add the melted agar solution.

[0077] Third, add 210ml of water or 210g of ice to the above solution, test the solution temperature, and when the temperature drops to 45℃, add the multivitamin solution and para-hydroxybenzoic acid, continue stirring until well mixed, and then pour into a suitable container for later use (e.g., Figure 1 (As shown).

[0078] Fourth, after the feed has cooled, cut it into appropriate squares or strips for feeding bean weevils (see...). Figure 2 ).

[0079] Newly hatched one-year-old beetle larvae will actively go to the feed to eat. Figure 3 Under conditions of 25℃ and 60-80% relative humidity, the yellowish-yellow color of newly hatched eggs will change to pale green after 2-3 days. Figure 3 B), after 3-5 days, peeling begins, marking the start of the second stage ( Figure 4 After 7-10 days, the skin begins to peel, marking the start of the 3rd stage (age 3). Figure 5 From hatching from the egg to molting and entering the 3rd instar, the fastest time for a bean weevil is only 7 days, and the slowest is 10 days.

[0080] Example 2: Preparation of artificial feed for young larvae of the soybean hawk moth

[0081] Prepare artificial feed for young larvae of the soybean hawk moth according to the feed formula shown in Table 2.

[0082] Table 2. Artificial feed formula for young larvae of the soybean hawk moth (1000g)

[0083]

[0084]

[0085] After preparing all the raw materials, weigh them according to the quantities in column three, and then prepare the artificial feed for bean weevils according to the order in column one. The specific preparation method is as follows:

[0086] Divide the pure water into three portions: one 300ml portion for dissolving the nutrient agar separately; one 300ml portion for dissolving other ingredients; and one 210ml portion for cooling (or you can use 210g of ice instead). Roast the corn flour at 80-120℃ to develop its aroma. Add the vitamin solution and para-hydroxybenzoic acid at around 45℃ and mix well.

[0087] Configure it by following these steps:

[0088] First, heat 300ml of water to 45℃, add the weighed nutrient agar, continue heating and stirring until the agar is completely dissolved, and set aside.

[0089] The second step is to add corn flour, bean leaf powder and salt to 300ml of water and stir well. Then start heating. Then add sucrose, casein and egg yolk powder in order and heat to 95℃ while stirring. Stop heating and add the melted agar solution.

[0090] Third, add 210ml of water or 210g of ice to the above solution, test the solution temperature, and when the temperature drops to 45℃, add the compound vitamin solution and para-hydroxybenzoic acid, continue to stir evenly, and then pour it into a suitable container for later use.

[0091] The fourth step is to cut the feed into appropriate squares or strips after it has cooled down for use in feeding bean weevils.

[0092] Example 3: Preparation of artificial feed for young larvae of the soybean hawk moth 2

[0093] Prepare artificial feed for young larvae of the soybean hawk moth according to the feed formula shown in Table 3.

[0094] Table 3. Artificial feed formula for young larvae of the soybean hawk moth (1000g)

[0095]

[0096]

[0097] After preparing all the raw materials, weigh them according to the quantities in column three, and then prepare the artificial feed for bean weevils according to the order in column one. The specific preparation method is as follows:

[0098] Divide the pure water into three portions: one 300ml portion for dissolving the nutrient agar separately; one 300ml portion for dissolving other ingredients; and one 210ml portion for cooling (or you can use 210g of ice instead). Roast the wheat germ powder at 80-120℃ to develop its aroma. Add the vitamin solution and para-hydroxybenzoic acid at around 45℃ and mix well.

[0099] Configure it by following these steps:

[0100] First, heat 300ml of water to 45℃, add the weighed nutrient agar, continue heating and stirring until the agar is completely dissolved, and set aside.

[0101] The second step is to add wheat germ powder, soybean leaf powder and salt to 300ml of water and stir well. Then start heating. Then add glucose, casein and egg yolk powder in sequence and heat to 95℃ while stirring. Stop heating and add the dissolved agar solution.

[0102] Third, add 210ml of water or 210g of ice to the above solution, test the solution temperature, and when the temperature drops to 45℃, add the compound vitamin solution and para-hydroxybenzoic acid, continue to stir evenly, and then pour it into a suitable container for later use.

[0103] The fourth step is to cut the feed into appropriate squares or strips after it has cooled down for use in feeding bean weevils.

[0104] Example 4: Rearing Experiment of Young Soybean Hawk Moth Larvae

[0105] The soybean hawk moth was reared using the artificial feed described in Examples 1-3. 200 eggs were introduced, and after hatching, the moth was fed the artificial feed as described in Examples 1-3 until it reached the third instar. Results showed that under conditions of 25℃ and 60-80% relative humidity, molting and entering the third instar occurred within 7-10 days. The fastest time from hatching to molting and entering the third instar was only 7 days, and the slowest was 10 days. (See...) Figure 5 Afterwards, the soybeans can be fed with artificial feed for older soybeans, or the feed can be directly placed on the soybeans in the field. They can then be marketed after 15-20 days. The results of feeding the formulas in each embodiment are shown in the table below:

[0106]

[0107] Since the survival rate of 1st and 2nd instar larvae under field rearing conditions is only 60%, this artificial feed can ensure that the survival rate of 1st instar larvae hatched is over 90% and the survival rate of 2nd instar larvae is over 95%. Therefore, using this artificial feed can significantly improve the rearing efficiency of bean weevils, speed up their entry into the market, and significantly improve production efficiency.

[0108] All documents mentioned in this invention are incorporated herein by reference as if each document were individually incorporated by reference. Furthermore, it should be understood that after reading the foregoing teachings of this invention, those skilled in the art can make various alterations or modifications to this invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. An artificial feed for young larvae of the soybean hawk moth, characterized in that, The artificial feed is composed of the following components: (1) Dispersion system: The dispersion system includes agar and pure water; (2) First complex: The first complex is: wheat germ powder, soybean leaf powder and salt; and the mass ratio of wheat germ powder, soybean leaf powder and salt is 2-4:4-6:0.8-1.2; (3) Second complex: The second complex is sucrose, casein and egg yolk powder; the mass ratio of sucrose, casein and egg yolk powder is 12-16:12-16:0.8-1.2; Furthermore, the mass ratio of the first complex to the second complex is 8-10:7-8; (4) Multivitamins and sorbic acid.

2. The artificial feed as described in claim 1, characterized in that, In the artificial feed, the ratio of the total mass of the dispersion system to the first complex and the second complex is m(dispersion system):m(first complex + second complex) = 1-3:8-12.

3. The artificial feed as described in any one of claims 1-2, characterized in that, By weight, the artificial feed consists of the following components: 15-20 parts nutrient agar, 800-850 parts pure water, 20-40 parts wheat germ powder, 40-60 parts soybean leaf powder, 8-12 parts salt, 30-40 parts sucrose, 30-40 parts casein, 2-3 parts egg yolk powder, 8-12 parts compound vitamin solution, and 0.2-0.8 parts sorbic acid.

4. The method for preparing artificial feed for young larvae of the soybean hawk moth as described in claim 1, characterized in that, Including the following steps: Dissolve the agar in pure water at 40-50℃ to obtain the first dispersion; The first complex and the second complex were dissolved in pure water to obtain the first solution; At 90-100℃, the first dispersion is added to the first solution to form a second dispersion; Water or ice is added to the second dispersion to cool it to 40-50°C, and then a compound vitamin solution and sorbic acid are added to obtain a third dispersion. The third dispersion is cooled and solidified to obtain the artificial feed.

5. The method as described in claim 4, characterized in that, In the method described, the first solution is prepared under heating conditions.

6. The method as described in claim 4, characterized in that, The wheat germ flour is pre-baked at 80-120℃.

7. The method as described in claim 4, characterized in that, The method further includes: after the artificial feed has cooled and solidified, cutting it into appropriate shapes for feeding.

8. A method for rearing young larvae of the soybean hawk moth, characterized in that, The method includes: feeding young larvae of the soybean hawk moth using the feed as described in any one of claims 1-3.

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