Dyeing machine and dyeing method

Abstract

The application provides a dyeing machine and a dyeing method. The dyeing method comprises the following steps: dyeing a sample smear in a dyeing box; providing water to clean the dyeing box; obtaining a time when the dyeing box is cleaned by the water; obtaining a current time; calculating a time length between the current time and the time when the dyeing box is cleaned by the water; and determining a cleaning mode of cleaning the dyeing box by using a cleaning reagent according to the time length. The dyeing machine can use the dyeing method to dye the sample smear, so that the amount of residual water in the dyeing box can be reduced, and the consumption amount of the cleaning reagent can be reduced.

Description

Technical Field

[0001] This invention relates to the field of sample staining technology, specifically to a staining machine and a staining method. Background Technology

[0002] Staining technology is one of the core technologies in staining machines or slide pushers. Typically, the sample is first mixed thoroughly, then collected and loaded onto a prepared glass slide. Next, it undergoes drying and other processes to form a sample smear, which is then stained.

[0003] One current method for staining sample smears involves placing each smear in a staining box, and then sequentially adding and removing various reagents and / or solutions to complete the staining process. The staining boxes are reusable; therefore, they need to be cleaned after each sample smear is stained.

[0004] Typically, staining boxes are cleaned directly with water. However, the remaining water in the staining box after cleaning can affect the reagents and / or solutions added to the box the next time. How to reduce this effect is one of the problems that slide staining machines need to improve or solve. Summary of the Invention

[0005] According to a first aspect, one embodiment provides a staining machine, comprising:

[0006] A staining box for receiving a staining solution and a sample smear, wherein the sample smear is stained in the staining box;

[0007] A liquid system is used to provide at least a dye solution to stain the sample smear loaded in the staining box, to provide water to clean the staining box, and to provide a cleaning reagent to clean the staining box.

[0008] A transfer mechanism for moving the staining box to a station that cooperates with the liquid path system, and

[0009] The control mechanism, which is communicatively connected to the transfer mechanism and the liquid circuit system, is configured as follows:

[0010] The moment when the staining box is washed with water is obtained;

[0011] Get the current time;

[0012] Calculate the time interval between the current moment and the moment when the dye box is washed with water;

[0013] The cleaning method for the dyeing box is determined based on the time duration.

[0014] According to a second aspect, one embodiment provides a staining method, comprising:

[0015] Stain the sample smears in the staining box;

[0016] Provide water to clean the dyeing box;

[0017] The moment when the staining box is washed with water is obtained;

[0018] Get the current time;

[0019] Calculate the time interval between the current moment and the moment when the dye box is washed with water;

[0020] The cleaning method for cleaning the staining box using cleaning reagents is determined based on the stated time length.

[0021] In some of the above embodiments, after rinsing the staining box with water, a cleaning reagent was added to the staining box to remove residual water and reduce the subsequent impact on the reagents and / or solutions in the staining box.

[0022] The inventors also discovered that the aforementioned methods for removing residual water increased the consumption of cleaning reagents. How to minimize the impact of residual water while avoiding excessive increases in cleaning reagent consumption was another problem the inventors considered.

[0023] To address this issue, in some of the aforementioned embodiments, after the staining box is washed with water, before the sample smear inside is stained again, it is neither stained directly nor has residual water been removed directly with cleaning reagent. Instead, the time between the current moment and the moment the staining box was washed with water is first obtained, and the cleaning method of the staining box is determined based on this time length. For example, the time length can be used to determine whether to provide cleaning reagent or how much reagent to provide. This reduces the amount of residual water in the staining box as much as possible, while also reducing the amount of cleaning reagent provided. This reduces costs and improves the subsequent staining effect. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of a dyeing machine according to one embodiment;

[0025] Figure 2 This is a schematic diagram of the transfer mechanism and liquid circuit system of a dyeing machine according to one embodiment;

[0026] Figure 3 This is a schematic diagram of the structure of a pusher machine according to one embodiment;

[0027] Figure 4 This is a schematic diagram of the pusher from another perspective of one embodiment.

[0028] Figure 5 A flowchart of a staining method according to one embodiment;

[0029] 10. Dyeing box;

[0030] 100. Fluid system; 110. First injection needle; 120. Second injection needle; 130. Third injection needle; 140. Fourth injection needle;

[0031] 200. Transfer mechanism; 210. First workstation; 220. Second workstation; 230. Third workstation; 240. Fourth workstation;

[0032] 300. Control mechanism;

[0033] 400. Slide loading mechanism;

[0034] 500. Sample collection and addition mechanism;

[0035] 600. Pushing mechanism. Detailed Implementation

[0036] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid obscuring the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0037] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0038] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0039] Existing methods for staining sample smears include the following steps after placing the sample smear into a staining box:

[0040] Cell prefixation solution is injected into the staining chamber to prefix the cells in the sample smear, a step aimed at maintaining cell morphology. The prefixation solution can be methanol-based or methanol-free. After prefixation, the prefixation solution is drained from the staining chamber, and then staining solution is injected back into the chamber to stain the sample smear. The staining solution can be a mixture of staining solution and buffer solution. After staining, the staining solution is drained from the chamber, and the stained sample smear is removed. The chamber is then rinsed with water, and the water is drained before reuse. This invention improves upon the existing method to enhance staining efficiency and reduce reagent consumption.

[0041] Please refer to Figure 1 and Figure 2 The embodiment shown provides a dyeing machine, including a dyeing box 10, a liquid circuit system 100, a transfer mechanism 200, and a control mechanism 300.

[0042] The staining box 10 is used to receive the staining solution and the sample smear. The sample smear is stained in the staining box 10. In this embodiment, one staining box 10 is used to hold one sample smear. The top of the staining box 10 has an opening through which the sample smear is inserted into the staining box 10.

[0043] The liquid system 100 is used to provide at least one dye solution to stain sample smears loaded in the staining container 10, to provide water to clean the staining container 10, and to provide a cleaning reagent to clean the staining container 10. The dye solution may include the aforementioned cell pre-fixation dye and staining dye, and the cleaning reagent may be methanol or alcohol, etc. In this embodiment, the liquid system 100 includes a first injection needle 110, a second injection needle 120, and a third injection needle 130. The first injection needle 110 is connected to a chamber containing the dye solution and is used to inject the dye solution into the staining container 10. The second injection needle 120 is connected to a chamber containing water and is used to inject water into the staining container 10. The third injection needle 130 is connected to a chamber containing the cleaning reagent and is used to inject the cleaning reagent into the staining container 10. In other embodiments, all or part of the dye solution, water, and cleaning reagent may share a single injection needle. The liquid system 100 of the present invention is not limited to the embodiments described above.

[0044] The transfer mechanism 200 is used to move the dyeing box 10 to a workstation that cooperates with the liquid system 100. In this embodiment, the transfer mechanism 200 is arranged in a disc shape and has multiple placement positions for placing the dyeing box 10. The transfer mechanism 200 can rotate and drive the dyeing box 10 in its placement position to rotate, so that it reaches the corresponding workstation. In this embodiment, the workstations include a first workstation 210, a second workstation 220, and a third workstation 230. A first injection needle 110 is located at the first workstation 210, a second injection needle 120 is located at the second workstation 220, and a third injection needle 130 is located at the third workstation 230. Each workstation may have a drain needle for discharging the corresponding liquid. As the transfer mechanism 200 rotates, the dyeing box 10 passes through the first workstation 210, the second workstation 220, and the third workstation 230 in sequence, and can return to the first workstation 210, and then the next cycle can begin. In other embodiments, the dyeing box 10 may also complete the dyeing at one station or other number of stations, and the transfer mechanism 200 is not limited to the transfer mechanism 200 of the above embodiments.

[0045] The control mechanism 300 is communicatively connected to the transfer mechanism 200 and the liquid system 100. It is configured to: control the liquid system 100 to supply water to the staining box 10 for cleaning; control the liquid system 100 to supply cleaning reagent to the staining box 10 for cleaning; and control the liquid system 100 to supply staining solution to the staining box 10 for staining the sample smears within the staining box 10. In other words, after being cleaned with water at the second station 220, the staining box 10 is then cleaned with cleaning reagent at the third station 230 before proceeding to the first station 210 for staining. By cleaning the staining box 10 with water followed by cleaning reagent, the amount of residual water in the staining box 10 can be reduced, preventing the residual water from diluting the staining solution.

[0046] In addition to the dyeing machine described above, the above embodiments can also be applied to a slide pusher, for example... Figure 3 and Figure 4 As shown, in addition to the staining box 10, liquid system 100 and control mechanism 300 mentioned above, the slide pusher also includes a slide loading mechanism 400, a sample collection and addition mechanism 500 and a slide pushing mechanism 600.

[0047] The slide loading mechanism 400 is used to extract slides and load them into the sample application position. In some embodiments, after the slide extraction operation is completed, operations such as left-right detection and slide cleaning can be performed before loading the slide. After loading, relevant information can be printed on the slide, and operations such as front-back detection can be performed.

[0048] The sample collection and loading mechanism 500 is used to collect samples and load them onto a glass slide at the loading position. The sample collection and loading mechanism 500 includes a sampling needle. In some embodiments, during sample extraction, the sample is first mixed, and then aspirated using the sampling needle. Depending on the sample container, aspiration can be either puncture aspiration (the sample container has a lid, and the sampling needle passes through the lid) or open aspiration (the sample container is open, and the sampling needle directly aspirates from the open portion). If necessary, blood sample information detection can be performed to obtain and compare information. In this embodiment, the same mechanism performs both aspiration and loading. In other embodiments, a separate aspiration mechanism can be used to perform aspiration, and a separate loading mechanism can be used to perform loading.

[0049] After the sample is loaded onto the glass slide, the slide-pushing mechanism 600 smooths the sample on the slide, pushing it into the shape of a blood film to form a sample smear (when the sample is blood, it can also be called a blood smear). Typically, after the slide-pushing action is completed, the blood film on the glass slide can be dried to stabilize its shape. In some embodiments, the glass slide can be flipped before drying the blood film to meet specific requirements. In some embodiments, the dried blood smear can also undergo a drying test to determine the drying effect of the blood film. In some embodiments, the dried blood smear can also undergo a blood film unfolding test to determine whether the blood film has unfolded and whether the unfolding state meets the requirements.

[0050] After the sample smear is prepared, it can be placed into the staining box 10 of the transfer mechanism 200 for staining.

[0051] In some embodiments, with Figure 1 The difference in the illustrated embodiment is that, when a dyeing box 10 is on the transfer mechanism 200, the control mechanism 300 is also used to obtain the time when the dyeing box 10 is cleaned with water. For example, the time when the second injection needle 120 injects water into the dyeing box 10 can be recorded, or the time when the transfer mechanism 200 moves the dyeing box 10 out of the second station 220 can be recorded as the time when the dyeing box 10 is cleaned with water can be recorded. The method of obtaining the time when the dyeing box 10 is cleaned with water is consistent for the same dyeing box 10. That is, if the last time the dyeing box 10 was cleaned with water was when the second injection needle 120 injected water into the dyeing box 10, then the next time it is cleaned with water is also when the second injection needle 120 injects water into the dyeing box 10. Furthermore, the control mechanism 300 also obtains the current time and calculates the time length between the current time and the time when the dyeing box 10 is cleaned with water, and then determines the cleaning method of the dyeing box 10 based on the time length.

[0052] For example, the control mechanism 300 determines the cleaning method of the staining box 10 based on the time length by: determining whether the time length meets a preset threshold; if the preset threshold is met, the control liquid system 100 does not provide cleaning reagent to clean the staining box 10, because if the time length meets the preset threshold, it means that most or all of the water remaining in the staining box 10 has evaporated, so there is no need to use cleaning reagent to remove the residual water; if the preset threshold is not met, the control liquid system 100 provides cleaning reagent to clean the staining box 10, and the timing of providing cleaning reagent can be before the sample smear is placed in the staining box 10, during the process of placing the sample smear in the staining box 10, or after the sample smear is placed in the staining box 10. In some embodiments, the preset threshold is two days. If the current time is more than two days away from the time the staining box 10 is cleaned with water, then the liquid system 100 will not provide cleaning reagent when the staining box 10 passes through the third station 230. The above-mentioned preset threshold can be determined based on factors related to the water evaporation rate, such as the temperature and / or humidity of the environment in which the staining box 10 is located.

[0053] For example, the control mechanism 300 determines the cleaning method of the staining box 10 based on the time length, including: determining the amount of cleaning reagent provided by the liquid system 100 based on the time length. For instance, the control mechanism 300 determines the time period corresponding to the time length, instructs the liquid system 100 to determine the amount of cleaning reagent to be provided based on the time period, and then instructs the liquid system 100 to provide cleaning reagent based on the determined amount. Here, there is at least one time period, and each time period has a pre-set amount of reagent. For example, the amount of reagent corresponding to a day can be half the amount of reagent corresponding to half a day. This is because the amount of residual water in the staining box 10 after being cleaned with water will be less than the amount of residual water after half a day, thus reducing the amount of cleaning reagent used and further reducing the amount of cleaning reagent used.

[0054] In some embodiments, if the number of dyeing boxes 10 on the transfer mechanism 200 is fixed, the above-mentioned time length for a certain dyeing box 10 is obtained as follows:

[0055] The moment when the dyeing box 10 is washed with water is obtained. In this embodiment, the moment when the dyeing box 10 is completely washed with water is taken as the moment when the dyeing box 10 is washed with water.

[0056] Assuming the total number of dyeing boxes 10 on the transfer mechanism 200 is N, after obtaining the time t1 when the dyeing box 10 is being washed with water, calculate the number n1 of dyeing boxes 10 that are currently being dyed or being washed. Each time the control mechanism 300 controls the liquid system 100 to provide dye solution, it means that a new dyeing box 10 has entered the dyeing stage, or that the dyeing machine has started a new dyeing process. Record the number of dyeing processes that are subsequently started as n2. When n1 + n2 = N, it means that the next dyeing box 10 to enter the dyeing stage is this dyeing box 10. At this time, the control mechanism 300 obtains the current time t2, and the time length = t2 - t1.

[0057] In some embodiments, the liquid system 100 is also used to provide methanol immersion staining boxes 10. The process of immersing staining boxes 10 in methanol is also referred to as methanol maintenance. Methanol maintenance can be performed periodically, and each methanol maintenance can target multiple or all staining boxes 10 on the transfer mechanism 200. For example, the liquid system 100 also includes a fourth injection needle 140 for injecting methanol into the staining box 10 to immerse it. Correspondingly, the transfer mechanism 200 can be provided with a fourth station 240. Each staining box 10 on the transfer mechanism 200 completes methanol immersion in the fourth station 240 before receiving sample smears again for staining. The control mechanism 300 determines whether to control the liquid system 100 to provide cleaning reagent to clean the staining box 10 based on whether the liquid system 100 has provided methanol immersion staining boxes 10. If the liquid system 100 has provided methanol immersion staining boxes 10, then the control mechanism 300 controls the liquid system 100 not to provide cleaning reagent to clean the staining box 10. In other words, if the staining box 10 is cleaned with water and then maintained with methanol, the aforementioned time duration is no longer required, or the time duration is no longer used as a basis for the cleaning method of the staining box 10. This is because methanol soaking can also remove residual water inside the staining box 10, and the staining box 10 that has been soaked in methanol does not need to be cleaned with cleaning reagents to remove residual water inside the staining box 10.

[0058] It should be noted that if the staining box 10 is cleaned with water and then maintained with methanol, then the above-mentioned time length need not be obtained, or the time length need not be used as the basis for the cleaning method of the staining box 10. Similarly, if the control mechanism 300 obtains the time length and the time length meets the preset threshold, then the control mechanism 300 need not determine whether the liquid circuit system 100 has provided methanol soaking for the staining box 10, and can directly control the liquid circuit system 100 not to provide cleaning reagent to clean the staining box 10.

[0059] The present invention also provides a staining method, such as Figure 5 As shown, the steps include:

[0060] Step 1000: Stain the sample smear in the staining box 10.

[0061] Step 2000: Provide water to clean the dyeing box 10.

[0062] In some embodiments, the following methods may be used: Figure 1 The staining machine shown stains sample smears and provides a water-washing staining box 10. The staining machine includes a liquid system 100 and a transfer mechanism 200.

[0063] The liquid system 100 is used to provide at least one staining solution to stain the sample smear loaded in the staining cartridge 10, and to provide water to clean the staining cartridge 10. The staining solution may include the aforementioned cell prefixation staining solution and staining solution, and the cleaning agent may be methanol or alcohol, etc. The transfer mechanism 200 is used to move the staining cartridge 10 to a working station that cooperates with the liquid system 100.

[0064] Step 3000: Obtain the moment when the staining box 10 is washed with water.

[0065] The moment when the dyeing box 10 is washed with water can be either the moment water is added to the dyeing box 10 or the moment when the dyeing box 10 is completely washed with water.

[0066] Step 4000: Obtain the current time.

[0067] Step 5000: Calculate the time between the current moment and the moment when the staining box 10 is washed with water.

[0068] In some embodiments, if the number of dyeing boxes 10 on the transfer mechanism 200 is fixed, the above-mentioned time length for a certain dyeing box 10 is obtained as follows:

[0069] The moment when the dyeing box 10 is washed with water is obtained. In this embodiment, the moment when the dyeing box 10 is completely washed with water is taken as the moment when the dyeing box 10 is washed with water.

[0070] Assuming the total number of dyeing boxes 10 on the transfer mechanism 200 is N, after obtaining the time t1 when the dyeing box 10 is being washed with water, calculate the number n1 of dyeing boxes 10 currently being dyed or washed. Each time the subsequent liquid system 100 provides dye solution, it means that a new dyeing box 10 enters the dyeing stage, or that the dyeing machine starts a new dyeing process. Record the number of subsequent dyeing processes as n2. When n1 + n2 = N, it means that the next dyeing box 10 to enter the dyeing stage is this dyeing box 10. At this time, obtain the current time t2, then the time length = t2 - t1.

[0071] Step 6000: Determine the cleaning method for the staining box 10 based on the time duration. The cleaning reagent can be methanol or alcohol, etc., and can also be provided by the liquid system 100.

[0072] In some embodiments, determining the cleaning method of the staining box 10 based on the time duration includes: determining whether the time duration meets a preset threshold; if it does, no cleaning reagent is provided to clean the staining box 10, because if the time duration meets the preset threshold, it means that most or all of the water remaining in the staining box 10 has evaporated, so there is no need to use cleaning reagent to remove the residual water; if the preset threshold is not met, cleaning reagent is provided to clean the staining box 10, and the timing of providing the cleaning reagent can be before, during, or after the sample smear is placed in the staining box 10. In some embodiments, the preset threshold is two days; if the current time is more than two days away from the time the staining box 10 is cleaned with water, no cleaning reagent is provided. The above-mentioned preset threshold can be determined based on factors related to the water evaporation rate, such as the temperature and / or humidity of the environment in which the staining box 10 is located.

[0073] In other embodiments, determining the cleaning method of the staining box 10 based on the time length includes: determining the amount of cleaning reagent to be provided based on the time length. For example, determining the time period corresponding to the time length, determining the amount of cleaning reagent to be provided based on the time period, and then providing the cleaning reagent based on the determined amount. The time period is at least one, and each time period has a pre-set amount of reagent. For example, the amount of reagent corresponding to a day can be half the amount of reagent corresponding to half a day. This is because the amount of residual water in the staining box 10 after being cleaned with water will be less than the amount of residual water after half a day, thus reducing the amount of cleaning reagent used and further reducing the amount of cleaning reagent used.

[0074] The above embodiments reduce the amount of residual water inside the staining box after it is washed with water, and also minimize the consumption of cleaning reagents. While reducing costs, they can improve the subsequent staining effect.

[0075] The above examples illustrate the present invention only to aid in understanding it and are not intended to limit the scope of the invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the principles of this invention.

Claims

1. A dyeing machine, characterized in that, include: A staining box for receiving a staining solution and a sample smear, wherein the sample smear is stained in the staining box; A liquid system is used to provide at least a dye solution to stain the sample smear loaded in the staining box, to provide water to clean the staining box, and to provide a cleaning reagent to clean the staining box. A transfer mechanism for moving the staining box to a station that cooperates with the liquid path system, and The control mechanism, which is communicatively connected to the transfer mechanism and the liquid circuit system, is configured as follows: Obtain the time when the staining box is washed with water; Get the current time; Calculate the time interval between the current moment and the moment the staining box is washed with water; The cleaning method for the dyeing box is determined based on the time duration.

2. The dyeing machine as described in claim 1, characterized in that, The step of determining the cleaning method of the staining box based on the time length includes: Determine whether the time length meets a preset threshold. If it does, control the liquid circuit system not to provide cleaning reagent to clean the staining box; otherwise, control the liquid circuit system to provide cleaning reagent to clean the staining box.

3. The dyeing machine as described in claim 2, characterized in that, The preset threshold is determined based on the temperature and / or humidity of the environment in which the dyeing box is located.

4. The dyeing machine as described in claim 2, characterized in that, Also includes: The system determines the time period corresponding to the time length, instructs the liquid circuit system to determine the amount of cleaning reagent to be provided based on the time period, and instructs the liquid circuit system to provide cleaning reagent based on the determined amount of reagent. The time period is at least one, and each time period has a preset amount of reagent.

5. The dyeing machine as described in claim 1, characterized in that, The step of determining the cleaning method of the staining box based on the time length includes: The amount of cleaning reagent provided by the liquid circuit system is determined based on the time length, specifically including: The system determines the time period corresponding to the time length, instructs the liquid circuit system to determine the amount of cleaning reagent to be provided based on the time period, and instructs the liquid circuit system to provide cleaning reagent based on the determined amount of reagent. The time period is at least one, and each time period has a preset amount of reagent.

6. The dyeing machine as described in claim 1, characterized in that, The cleaning agent is methanol.

7. The dyeing machine as described in claim 1, characterized in that, The liquid circuit system is also used to provide methanol immersion in the dyeing box; The control mechanism is also configured to: Based on whether the liquid circuit system has provided methanol to soak the staining box, determine whether to control the liquid circuit system to provide cleaning reagent to clean the staining box; If the liquid circuit system has provided methanol to soak the staining box, then the liquid circuit system is controlled not to provide cleaning reagent to clean the staining box.

8. A dyeing machine, characterized in that, include: A staining box for receiving a staining solution and a sample smear, wherein the sample smear is stained in the staining box; A liquid system is used to provide at least one dye solution to stain the sample smear loaded in the staining box, to provide water to clean the staining box, to provide a cleaning reagent to clean the staining box, and to provide methanol to maintain the staining box. A transfer mechanism for moving the staining box to a station that cooperates with the liquid path system, and The control mechanism, which is communicatively connected to the transfer mechanism and the liquid circuit system, is configured as follows: Determine whether the staining box has undergone methanol maintenance. If the staining box has undergone methanol maintenance, control the liquid circuit system not to provide the cleaning reagent to clean the staining box; otherwise, control the liquid circuit system to provide the cleaning reagent to clean the staining box.

9. A staining method, characterized in that, include: Stain the sample smears in the staining box; Provide water to clean the dyeing box; Obtain the time when the staining box is washed with water; Get the current time; Calculate the time interval between the current moment and the moment the staining box is washed with water; The cleaning method for cleaning the staining box using cleaning reagents is determined based on the stated time length.

10. The method as described in claim 9, characterized in that, The step of determining the cleaning method for cleaning the staining box using cleaning reagents based on the time length includes: Determine whether the time length meets a preset threshold. If not, provide cleaning reagent to clean the staining box; otherwise, do not provide cleaning reagent to clean the staining box.

11. The method as described in claim 10, characterized in that, Also includes: The amount of cleaning reagent provided is determined based on the time length, specifically including: Determine the time period corresponding to the time length, and determine the amount of cleaning reagent to be provided based on the time period, wherein the time period is at least one, and each time period has a preset amount of reagent.

12. The method as described in claim 9, characterized in that, The step of determining the cleaning method for cleaning the staining box using cleaning reagents based on the time length includes: Determining the amount of cleaning reagent to be provided based on the time length specifically includes: determining the time period corresponding to the time length, determining the amount of cleaning reagent to be provided based on the time period, and providing the cleaning reagent based on the determined amount of reagent, wherein the time period is at least one, and each time period has a preset amount of reagent.

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