A growth factor extraction device

Abstract

The application discloses a growth factor extraction device, which comprises a centrifuge, an aeration mechanism and a plurality of centrifugal units; the centrifugal table of the centrifuge is provided with a plurality of mounting holes, and the bottom of each mounting hole is provided with an aeration connector; the aeration mechanism is used for aeration to each aeration connector respectively; the centrifugal unit comprises a lower cylindrical body and an upper cylindrical body; the lower cylindrical body is provided with a centrifugal cavity, the centrifugal cavity is adapted to a piston, the lower end of the lower cylindrical body is coaxially provided with a connector, the lower end of the lower cylindrical body is inserted into a mounting hole and is threadedly connected with the aeration connector through the connector; the lower end of the upper cylindrical body is detachably and rotatably connected with the upper end of the lower cylindrical body, and the upper cylindrical body is provided with a white blood cell cavity, a platelet cavity and a red blood cell cavity. The application can automatically separate red blood cells, white blood cells and platelets after blood centrifugal stratification, reduces the separation difficulty, improves the separation efficiency, realizes more thorough separation, and the platelet gel body is not easily damaged in the separation process.

Description

Technical Field

[0001] This invention relates to the field of growth factor extraction technology, and more specifically to a growth factor extraction device. Background Technology

[0002] Growth factors (CGFs) are a class of polypeptides that regulate cell growth and other cellular functions by binding to specific, high-affinity cell membrane receptors. They are found in platelets, various adult and embryonic tissues, and most cultured cells. They can be injected alone or in combination with other biological materials into hard tissue defects or soft tissue injuries to repair defects, induce growth, accelerate the healing of local wounds, and improve the quality of healing, thus improving and enhancing tissue regeneration.

[0003] Blood contains red blood cells, white blood cells, platelets, etc., and growth factors are mainly found in platelets. Therefore, when extracting growth factors, it is necessary to separate red blood cells, white blood cells and platelets in the blood. Currently, the specific method for extracting growth factors from blood is as follows: (1) Collect venous blood in centrifuge tubes; (2) Put the collected venous blood into a centrifuge and centrifuge to separate the layers, obtaining a white blood cell layer, a platelet layer and a red blood cell layer, where the white blood cell layer is the upper clear liquid, the platelet layer is the intermediate gel, and the red blood cell layer is the lower gel; (3) Remove the centrifuge tube from the centrifuge, then pour out the upper clear liquid in the centrifuge tube, then pour out the gel in the centrifuge tube, and finally use tweezers to pick out the platelet gel.

[0004] In existing technologies, after blood is centrifuged and separated, red blood cells, white blood cells, and platelets need to be manually separated. This is not only cumbersome and requires highly skilled operators, but also suffers from incomplete separation and easy destruction of platelet gels. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a growth factor extraction device that can automatically separate red blood cells, white blood cells, and platelets after blood is centrifuged and separated, reducing the difficulty of separation, improving separation efficiency, and making the separation more thorough. Furthermore, platelet gels are not easily destroyed during the separation process.

[0006] This invention provides a growth factor extraction device, comprising:

[0007] A centrifuge, wherein the centrifuge table is provided with a plurality of mounting holes, and each mounting hole is provided with an air inlet at the bottom;

[0008] An inflation mechanism is provided for inflating each of the inflation joints.

[0009] A plurality of centrifuge units, each centrifuge unit comprising a lower column and an upper column; the lower column has an eccentrically disposed centrifuge chamber extending downward from its upper end, a piston adapted within the centrifuge chamber, and an interface coaxially disposed at the lower end of the lower column communicating with the centrifuge chamber; the lower end of the lower column is inserted into a mounting hole, and the interface at the lower end of the lower column is threadedly connected to an inflation connector at the bottom of the mounting hole; the lower end of the upper column abuts against the upper end of the lower column and is detachably and rotatably connected to the upper end of the lower column; the upper column has a white blood cell chamber, a platelet chamber, and a red blood cell chamber extending upward from its lower end, and by rotating the upper column, the lower ends of the white blood cell chamber, platelet chamber, and red blood cell chamber can be sequentially aligned with the upper end of the centrifuge chamber.

[0010] Furthermore, a rotating sleeve is rotatably connected to the lower end of the upper columnar body, and the lower end of the rotating sleeve is threadedly connected to the upper end of the lower columnar body.

[0011] Furthermore, the upper end of the upper column is provided with three vent holes that are respectively connected to the white blood cell cavity, the platelet cavity and the red blood cell cavity, and each vent hole is equipped with a sealing plug.

[0012] Furthermore, the lower end of the upper columnar body has a sealing position, which can be connected to the upper end of the centrifuge chamber.

[0013] Furthermore, the lower end of the upper columnar body is fixed with a first sealing ring, a second sealing ring, a third sealing ring and a fourth sealing ring. The first sealing ring is located around the white blood cell cavity, the second sealing ring is located around the platelet cavity, the third sealing ring is located around the red blood cell cavity, and the fourth sealing ring is located around the sealing position.

[0014] Furthermore, it also includes a drive mechanism, which includes a drive motor and a drive gear. The drive motor is located at the center of the top of the centrifuge platform, and the drive gear is coaxially mounted on the output shaft of the drive motor. Each centrifuge unit surrounds the drive mechanism, and each centrifuge unit has a driven gear on its upper columnar body that meshes with the drive gear.

[0015] Furthermore, the inflation mechanism includes a pressure tank located inside the centrifuge, a main air supply pipe connected to the pressure tank, and branch air supply pipes connected between the main air supply pipe and each inflation connector, with a solenoid valve installed on each branch air supply pipe.

[0016] Furthermore, it also includes a controller;

[0017] The top of the centrifuge table is provided with several image acquisition units that correspond one-to-one with each of the centrifuge units. The image acquisition units are used to acquire images of the junction between the lower column and the upper column of each centrifuge unit.

[0018] The input terminal of the controller is electrically connected to each of the image acquisition units, and the output terminal of the controller is electrically connected to the drive motor and each of the solenoid valves.

[0019] The beneficial effects of this invention are reflected in:

[0020] When using this device to extract platelet-growth factor, the drawn venous blood is placed in the centrifuge chamber of the lower column. The lower column is then inserted into the mounting hole, and the lower end of the lower column is tightened to the air inlet at the bottom of the mounting hole. Simultaneously, the lower column is connected to the upper end of the centrifuge. The centrifuge is then started to centrifuge and separate the blood within the centrifuge chamber, forming a layer of white blood cells, platelets, and red blood cells arranged sequentially from top to bottom. After the blood has separated, the upper column is rotated so that the lower end of the white blood cell chamber within the upper column aligns with the upper end of the centrifuge chamber. The air inlet mechanism is then controlled to inflate the corresponding air inlet, causing the piston within the centrifuge chamber to rise under air pressure. The piston pushes the white blood cells, platelets, and red blood cells within the centrifuge chamber upwards slowly. Once the white blood cells are pressed into the white blood cell chamber, the upper column is rotated at an angle so that the white blood cells within the upper column... The lower end of the platelet cavity is aligned with the upper end of the centrifuge chamber. The inflation mechanism is then continuously controlled to inflate the corresponding inflation connectors, causing the piston within the centrifuge chamber to rise further. The piston pushes the platelets and red blood cells within the centrifuge chamber upwards slowly. Once the platelets are pressed into the platelet cavity, the upper column is rotated at an angle so that the lower end of the red blood cell cavity within the upper column is aligned with the upper end of the centrifuge chamber. The inflation mechanism is then continuously controlled to inflate the corresponding inflation connectors, causing the piston within the centrifuge chamber to rise further. The piston pushes the red blood cells within the centrifuge chamber upwards slowly. Once the red blood cells are pressed into the platelet cavity, the centrifuge unit is removed. The centrifuge unit is then inverted, and finally, the upper column is removed from the lower column. In this way, white blood cells, platelets, and red blood cells are obtained from the white blood cell cavity, platelet cavity, and red blood cell cavity of the upper column, respectively, thus achieving the separation of the layers after blood centrifugation.

[0021] Therefore, compared with the prior art, in the process of extracting growth factors, the centrifugation and separation of blood layers in this application are completed on a centrifuge table. It can automatically separate red blood cells, white blood cells and platelets after the blood is centrifuged and separated, which reduces the difficulty of separation, improves the separation efficiency, and makes the separation more thorough. Moreover, the platelet gel is not easily destroyed during the separation process. Attached Figure Description

[0022] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0023] Figure 1 This is a top view of an embodiment of the present invention;

[0024] Figure 2 for Figure 1 AA section view;

[0025] Figure 3 This is a bottom view of the upper columnar body according to an embodiment of the present invention;

[0026] Figure 4 This is a diagram showing the usage state of the centrifuge unit according to an embodiment of the present invention (I);

[0027] Figure 5 This is a diagram showing the usage state of the centrifuge unit according to an embodiment of the present invention (II);

[0028] Figure 6 This is a diagram showing the usage state of the centrifuge unit according to an embodiment of the present invention (III);

[0029] Figure 7 This is a diagram showing the usage state of the centrifuge unit according to an embodiment of the present invention (IV);

[0030] Figure 8 This is a usage state diagram (V) of the centrifuge unit according to an embodiment of the present invention;

[0031] Figure 9 This is a diagram showing the usage state of the centrifuge unit according to an embodiment of the present invention (VI).

[0032] In the attached diagram, 100-centrifuge stand; 110-mounting hole; 111-inflation connector; 200-inflation mechanism; 210-pressure tank; 220-main gas supply pipe; 230-branch gas supply pipe; 240-solenoid valve; 300-centrifuge unit; 310-lower column; 311-centrifuge chamber; 312-piston; 313-interface; 320-upper column; 321-leukocyte chamber; 322-platelet chamber; 323-... - Red blood cell cavity; 324- Rotating sleeve; 325- Vent hole; 326- Sealing plug; 327- Sealing position; 328- First sealing ring; 329- Second sealing ring; 3210- Third sealing ring; 3211- Fourth sealing ring; 400- Drive mechanism; 411- Drive motor; 412- Driving gear; 413- Driven gear; 500- Image acquisition unit; P- Platelet; R- Red blood cell; W- White blood cell. Detailed Implementation

[0033] The embodiments of the technical solution of the present invention will now be described in detail with reference to the accompanying drawings. These embodiments are merely illustrative of the technical solution of the present invention and are therefore intended to limit the scope of protection of the present invention.

[0034] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0035] like Figures 1-9 As shown, an embodiment of the present invention provides a growth factor extraction device, including a centrifuge, an aeration mechanism 200, and several centrifugation units 300.

[0036] The centrifuge platform 100 has several mounting holes 110, and each mounting hole 110 has an air inlet connector 111 at its bottom.

[0037] The inflation mechanism 200 is used to inflate each inflation connector 111. Specifically, the inflation mechanism 200 includes a pressure tank 210 located in the centrifuge 100, a main air supply pipe 220 connected to the pressure tank 210, and a branch air supply pipe 230 connected between the main air supply pipe 220 and each inflation connector 111. Each branch air supply pipe 230 is equipped with a solenoid valve 240. When the solenoid valve 240 on each branch air supply pipe 230 is opened, the inflation connector 111 connected to the corresponding branch air supply pipe 230 can be inflated.

[0038] The centrifuge unit 300 includes a lower columnar body 310 and an upper columnar body 320. An eccentrically positioned centrifuge chamber 311 extending downwards from its upper end is provided within the lower columnar body 310. A piston 312 is fitted inside the centrifuge chamber 311. An interface 313 communicating with the centrifuge chamber 311 is coaxially provided at the lower end of the lower columnar body 310. The lower end of the lower columnar body 310 is inserted into a mounting hole 110, and the interface 313 at the lower end of the lower columnar body 310 is threadedly connected to an inflation connector 111 at the bottom of the mounting hole 110. The lower end of the upper column 320 abuts against the upper end of the lower column 310 and is detachably and rotatably connected to the upper end of the lower column 310. The upper column 320 is provided with a white blood cell cavity 321, a platelet cavity 322 and a red blood cell cavity 323 extending upward from its lower end. By rotating the upper column 320, the lower ends of the white blood cell cavity 321, the platelet cavity 322 and the red blood cell cavity 323 can be sequentially connected to the upper end of the centrifuge cavity 311.

[0039] In one specific embodiment, a rotating sleeve 324 is rotatably connected to the lower end of the upper columnar body 320, and the lower end of the rotating sleeve 324 is threadedly sleeved with the upper end of the lower columnar body 310. When connecting the upper columnar body 320 and the lower columnar body 310, the upper columnar body 320 and the lower columnar body 310 can be aligned first, and then, while keeping the upper columnar body 320 stationary, the rotating sleeve 324 under the upper columnar body 320 is tightened onto the lower columnar body 310, thereby achieving accurate alignment and fixed connection of the upper columnar body 320 and the lower columnar body 310.

[0040] When using this device to extract platelet P growth factor, such as Figure 4 As shown, the drawn venous blood is placed in the centrifuge chamber 311 of the lower column 310. Then, the lower column 310 is inserted into the mounting hole 110, and the interface 313 at the lower end of the lower column 310 is tightened to the air inlet connector 111 at the bottom of the mounting hole 110. At the same time, the lower column 310 is connected to the upper end of the lower column 310. Then, the centrifuge can be started to centrifuge and separate the blood in the centrifuge chamber 311 to form a layer of white blood cells (W), platelets (P), and red blood cells (R) arranged from top to bottom in the centrifuge chamber 311 (e.g., white blood cell W layer, platelet P layer, and red blood cell R layer). Figure 5 As shown), after the blood is centrifuged and separated, the upper column 320 is rotated so that the lower end of the white blood cell cavity 321 inside the upper column 320 is aligned with the upper end of the centrifugation cavity 311. Then, the inflation mechanism 200 is controlled to inflate the corresponding inflation connector 111, causing the piston 312 inside the centrifugation cavity 311 to rise under the action of air pressure. The piston 312 pushes the white blood cells W, platelets P, and red blood cells R inside the centrifugation cavity 311 to rise slowly. When the white blood cells W are forced into the white blood cell cavity 321 (as shown), Figure 6 (As shown), rotate the upper column 320 by an angle so that the lower end of the platelet cavity 322 inside the upper column 320 is aligned with the upper end of the centrifuge cavity 311. Then, continue to control the inflation mechanism 200 to inflate the corresponding inflation connector 111, so that the piston 312 inside the centrifuge cavity 311 continues to rise. The piston 312 pushes the platelets P and red blood cells R inside the centrifuge cavity 311 to rise slowly. When the platelets P are pressed into the platelet cavity 322 (as shown), Figure 7 (As shown), rotate the upper column 320 by an angle so that the lower end of the red blood cell cavity 323 inside the upper column 320 is aligned with the upper end of the centrifuge cavity 311. Then, continue to control the inflation mechanism 200 to inflate the corresponding inflation connector 111, so that the piston 312 inside the centrifuge cavity 311 continues to rise. The piston 312 pushes the red blood cells R inside the centrifuge cavity 311 to rise slowly. When the red blood cells R are pressed into the platelet cavity 322 (as shown), Figure 8 As shown), remove the centrifuge unit 300, and then invert the centrifuge unit 300 (as shown). Figure 9As shown), the upper column 320 is finally removed from the lower column 310, so that white blood cells W, platelets P and red blood cells R are obtained in the white blood cell cavity 321, platelet cavity 322 and red blood cell cavity 323 of the upper column 320, respectively, thereby realizing the separation of each layer after blood centrifugation.

[0041] Therefore, compared with the prior art, in the process of extracting growth factors, the centrifugation and separation of blood layers in this application are completed on the centrifuge table 100. It can automatically separate red blood cells (R), white blood cells (W), and platelets (P) after the blood is centrifuged and separated, which reduces the difficulty of separation, improves the separation efficiency, and makes the separation more thorough. Moreover, the platelet P gel is not easily destroyed during the separation process.

[0042] In this embodiment, the upper end of the upper columnar body 320 is also provided with three vent holes 325 that are respectively connected to the white blood cell cavity 321, the platelet cavity 322 and the red blood cell cavity 323. Each vent hole 325 is equipped with a sealing plug 326. During the process of white blood cell W being pushed into the white blood cell cavity 321, platelet P being pushed into the platelet cavity 322 and red blood cell R being pushed into the red blood cell cavity 323, the three vent holes 325 can exhaust air from the white blood cell cavity 321, the platelet cavity 322 and the red blood cell cavity 323 respectively. When the centrifuge unit 300 is inverted, each vent hole 325 is blocked by the sealing plug 326, which can prevent liquid from flowing out of the vent hole 325.

[0043] In this embodiment, the lower end of the upper column 320 has a sealing position 327, which can be connected to the upper end of the centrifuge chamber 311. During centrifugation, the sealing position 327 of the upper column 320 seals the upper end of the centrifuge chamber 311, thereby preventing blood leakage.

[0044] Preferably, the lower end of the upper columnar body 320 is fixed with a first sealing ring 328, a second sealing ring 329, a third sealing ring 3210, and a fourth sealing ring 3211. The first sealing ring 328 is disposed around the leukocyte cavity 321 to prevent leakage between the lower end of the leukocyte cavity 321 and the lower columnar body 310. The second sealing ring 329 is disposed around the platelet cavity 322 to prevent leakage between the lower end of the platelet cavity 322 and the lower columnar body 310. The third sealing ring 3210 is disposed around the erythrocyte cavity 323 to prevent leakage between the lower end of the erythrocyte cavity 323 and the lower columnar body 310. The fourth sealing ring 3211 is disposed around the sealing position 327 to prevent leakage between the sealing position 327 and the lower columnar body 310.

[0045] During the separation of white blood cells (W), platelets (P), and red blood cells (R), the upper column 320 can be rotated manually or automatically by a drive mechanism 400. In this embodiment, the rotation of the upper column 320 is preferably driven by the drive mechanism 400. Specifically, the drive mechanism 400 includes a drive motor 411 and a drive gear 412. The drive motor 411 is located at the center of the top of the centrifuge stage 100, and the drive gear 412 is coaxially mounted on the output shaft of the drive motor 411. Each centrifuge unit 300 surrounds the drive mechanism 400, and each centrifuge unit 300 has a driven gear 413 on the outside of its upper column 320 that meshes with the drive gear 412. This drive mechanism 400 can satisfy the requirement of driving the upper column 320 to rotate without affecting the insertion and removal of each centrifuge unit 300.

[0046] This embodiment also includes a controller. The top of the centrifuge table 100 is provided with a plurality of image acquisition units 500 corresponding one-to-one with each centrifuge unit 300. The image acquisition units 500 are used to acquire images of the junction of the lower column 310 and the upper column 320 of each centrifuge unit 300. The input terminal of the controller is electrically connected to each image acquisition unit 500, and the output terminal of the controller is electrically connected to the drive motor 411 and each solenoid valve 240 respectively.

[0047] In this embodiment, the lower columnar body 310 and the upper columnar body 320 are made of transparent material, making their internal structures clearly visible. After the blood is centrifuged and separated, the controller outputs a signal to control the drive motor 411 to rotate the upper columnar body 320 by an angle, aligning the lower end of the leukocyte cavity 321 with the upper end of the centrifugation cavity 311. Then, the controller outputs a signal to control the solenoid valve 240 to open, driving the piston 312 to push the blood in the centrifugation cavity 311 upwards. During this process, the image acquisition unit 500 acquires images of the junction between the lower columnar body 310 and the upper columnar body 320 of each centrifugation unit 300. The controller identifies the images acquired by the image acquisition unit 500. When it is found that the separation position of the leukocyte W layer and the platelet P layer reaches the junction between the lower columnar body 310 and the upper columnar body 320, the controller outputs a signal to control the solenoid valve 240 to close, and then outputs a signal to control the drive motor. The controller drives the upper column 320 to rotate at an angle, so that the lower end of the platelet cavity 322 is aligned with the upper end of the centrifuge cavity 311. Then, the controller outputs a signal to control the solenoid valve 240 to open, so as to drive the piston 312 to continue to push the blood in the centrifuge cavity 311 upward. When the separation position of the platelet P layer and the erythrocyte R layer is found to reach the junction of the lower column 310 and the upper column 320, the controller outputs a signal to control the solenoid valve 240 to close, and then outputs a signal to control the drive motor 411 to drive the upper column 320 to rotate at an angle, so that the lower end of the erythrocyte cavity 323 is aligned with the upper end of the centrifuge cavity 311. Then, the controller outputs a signal to control the solenoid valve 240 to open, so as to drive the piston 312 to continue to push the blood in the centrifuge cavity 311 upward, until the erythrocyte R in the centrifuge cavity 311 is completely pushed into the erythrocyte cavity 323, thereby realizing the fully automatic separation of white blood cells W, platelets P and erythrocyte R.

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention.

Claims

1. A growth factor extraction device, characterized in that, include: A centrifuge, wherein the centrifuge table is provided with a plurality of mounting holes, and each mounting hole is provided with an air inlet at the bottom; An inflation mechanism is provided for inflating each of the inflation joints. A plurality of centrifuge units, each centrifuge unit comprising a lower column and an upper column; the lower column has an eccentrically disposed centrifuge chamber extending downward from its upper end, a piston adapted within the centrifuge chamber, and an interface coaxially disposed at the lower end of the lower column communicating with the centrifuge chamber; the lower end of the lower column is inserted into a mounting hole, and the interface at the lower end of the lower column is threadedly connected to an inflation connector at the bottom of the mounting hole; the lower end of the upper column abuts against the upper end of the lower column and is detachably and rotatably connected to the upper end of the lower column; the upper column has a white blood cell chamber, a platelet chamber, and a red blood cell chamber extending upward from its lower end; by rotating the upper column, the lower ends of the white blood cell chamber, platelet chamber, and red blood cell chamber can be sequentially aligned with the upper end of the centrifuge chamber; It also includes a drive mechanism, which includes a drive motor and a drive gear. The drive motor is located at the center of the top of the centrifuge platform, and the drive gear is coaxially mounted on the output shaft of the drive motor. Each centrifuge unit surrounds the drive mechanism, and each centrifuge unit has a driven gear that meshes with the drive gear on its upper columnar body.

2. The growth factor extraction device according to claim 1, characterized in that, The lower end of the upper column is rotatably connected to a rotating sleeve, and the lower end of the rotating sleeve is threadedly connected to the upper end of the lower column.

3. The growth factor extraction device according to claim 1, characterized in that, The upper end of the upper column is also provided with three vents that are respectively connected to the white blood cell cavity, the platelet cavity and the red blood cell cavity, and each vent is equipped with a sealing plug.

4. The growth factor extraction device according to claim 1, characterized in that, The lower end of the upper column has a sealing position, which can be connected to the upper end of the centrifuge chamber.

5. The growth factor extraction device according to claim 4, characterized in that, The lower end of the upper columnar body is fixed with a first sealing ring, a second sealing ring, a third sealing ring and a fourth sealing ring. The first sealing ring is located around the white blood cell cavity, the second sealing ring is located around the platelet cavity, the third sealing ring is located around the red blood cell cavity, and the fourth sealing ring is located around the sealing position.

6. The growth factor extraction apparatus according to claim 1, characterized in that, The inflation mechanism includes a pressure tank located inside the centrifuge, a main air supply pipe connected to the pressure tank, and branch air supply pipes connected between the main air supply pipe and each inflation connector. Each branch air supply pipe is equipped with a solenoid valve.

7. The growth factor extraction apparatus according to claim 6, characterized in that, It also includes the controller; The top of the centrifuge table is provided with several image acquisition units that correspond one-to-one with each of the centrifuge units. The image acquisition units are used to acquire images of the junction between the lower column and the upper column of each centrifuge unit. The input terminal of the controller is electrically connected to each of the image acquisition units, and the output terminal of the controller is electrically connected to the drive motor and each of the solenoid valves.

Images