Cage assembly for animal test subjects

Abstract

A cage assembly (100) may have at least one enclosure. Each enclosure may have: a floor (104) defining a floor area having a principal dimension; and a cover (120, 178) having a bottom surface. The distance between the bottom surface of the cover and the floor may define the cage height. At least one sidewall (108) may extend between the floor and the cover. The ratio of the cage height of each enclosure to the principal dimension of the floor area may be at least 0.70.

Description

[0001] Cross-references to related applications

[0002] This application claims priority and benefit to the filing dates of U.S. Provisional Patent Application No. 62 / 951,599, filed December 20, 2019, and U.S. Provisional Patent Application No. 63 / 104,788, filed October 23, 2020, the entire contents of each of which are incorporated herein by reference. Technical Field

[0003] This invention relates to systems, apparatus, and methods for testing and using tumor treatment fields (TTFields). This disclosure includes descriptions of cage assemblies, treatment assemblies, and rotary joint systems for use with animal test subjects. Background Technology

[0004] A tumor therapeutic field, or TTField, is a low-intensity (e.g., 1-3 V / cm) alternating electric field in the mid-frequency range (100-300 kHz). This non-invasive treatment targets solid tumors and is described in U.S. Patent No. 7,565,205, which is incorporated herein by reference in its entirety. TTField disrupts cell division during mitosis through physical interactions with key molecules. TTField therapy is an approved monotherapy for recurrent glioblastoma and an approved combination therapy with chemotherapy for newly diagnosed patients. These electric fields are non-invasively generated by an array of transducers (i.e., an electrode array) placed directly on the patient's scalp. TTFields also appear to be beneficial for treating tumors in other parts of the body. Laboratory studies have begun testing mid-frequency alternating electric fields (tumor therapeutic fields, or TTFields) on subcutaneous and in situ tumors located in the trunk of small animals (e.g., mice). Summary of the Invention

[0005] In various respects, a cage assembly for animal test subjects is described herein. Optionally, the cage assembly can be used to house one or more animal test subjects undergoing TTField or control treatment.

[0006] A cage assembly may include at least one enclosure. Each enclosure may have: a floor defining a floor area having a principal dimension; and a cover having a bottom surface. The distance between the bottom surface of the cover and the floor may define the cage height. At least one sidewall may extend between the floor and the cover. The ratio of the cage height of each enclosure to the principal dimension of the floor area may be at least 0.70.

[0007] The at least one enclosure may include a first enclosure and a second enclosure.

[0008] The at least one enclosure may consist of a first enclosure and a second enclosure.

[0009] The first enclosure and the second enclosure may share a common sidewall that separates the floor area of ​​the first enclosure from the floor area of ​​the second enclosure.

[0010] The common sidewall may define at least one opening between the first enclosure and the second enclosure.

[0011] Each of the first enclosure and the second enclosure may include a respective shielding sub-assembly extending inwardly from the common sidewall within the enclosure. The shielding sub-assembly may at least partially surround an opening within the common sidewall.

[0012] The floor of each enclosure may define corners, and each corner of the floor of the enclosure shall have a radius of at least 17 mm.

[0013] The main dimensions of the floor area of ​​each enclosure may not exceed 250 mm.

[0014] The floors of the first and second enclosures can be constructed as a single unit.

[0015] The covers of the first enclosure and the second enclosure can be constructed as a whole as a cover assembly.

[0016] The cover assembly may include a first opening and a second opening respectively providing communication with a first enclosure and a second enclosure. The first opening may be configured to provide communication with a first cable, and the second opening may be configured to provide communication with a second cable.

[0017] The cover assembly may include a first rotary connector assembly and a second rotary connector assembly positioned in a covering relationship with the first opening and the second opening, respectively. The first rotary connector assembly may be configured to receive a proximal portion of the first cable, and the second rotary connector assembly may be configured to receive a proximal portion of the second cable.

[0018] Each of the first and second rotary joint assemblies may include a motor housing and a motor received within the motor housing. The motor of the first rotary joint assembly may be configured to be coupled to a first cable to allow adjustment of the first cable. The motor of the second rotary joint assembly may be configured to be coupled to a second cable to allow adjustment of the second cable.

[0019] The at least one enclosure may include a first enclosure. The cover may include a rotary connector assembly positioned in a covering relationship with the first enclosure. The cover may further include an opening providing communication with the first enclosure. This opening may be configured to provide communication between the cable and the rotary connector assembly.

[0020] The rotary joint assembly may include a motor. The motor may be configured to be coupled to the proximal portion of the cable to allow adjustment of the cable's movement.

[0021] The at least one sidewall of each of the first enclosure and the second enclosure may further include: a front sidewall; a rear sidewall; and a transverse sidewall that is opposite to the common sidewall and extends between the front and rear sidewalls.

[0022] At least a portion of the front sidewalls of the first enclosure and the second enclosure may be constructed integrally.

[0023] The rear sidewalls of the first and second enclosures can be constructed as a single unit.

[0024] The front sidewalls of the first and second enclosures may include: a base portion fixed to the transverse sidewalls of the first and second enclosures; and a door pivotally connected to the base portion. The door may be configured to move about and between: a closed position, wherein the door cooperates with the front, transverse, and rear sidewalls of the first and second enclosures and the cover to enclose the interior space within the cage assembly; and an open position, wherein the interior space of the cage assembly is accessible.

[0025] The door can be pivotally connected to the base section via hinge connectors.

[0026] The cage assembly may further include a latch mechanically coupled to the door. The latch may be movable about both and between: a latched position that prevents pivoting movement of the door when the door is in the closed position; and an unlocked position that allows pivoting movement of the door relative to the base portion.

[0027] The floor, cover, and at least one sidewall of each enclosure may comprise polycarbonate.

[0028] The cover of each enclosure and at least a portion of the at least one sidewall may be transparent.

[0029] The cover can define the opening that is configured to receive the wire.

[0030] The floor of each enclosure may include mattress material.

[0031] At least one sidewall of each enclosure may include a ventilation opening.

[0032] The cage assembly may further include at least one filter configured to cover at least one ventilation opening on the at least one sidewall.

[0033] The cage assembly may further include a frame configured to mechanically attach the filter to the at least one sidewall.

[0034] The cage assembly can be sealed such that all or substantially all of the ventilation to each enclosure travels through the at least one filter before entering the ventilation opening.

[0035] Each enclosure can have sidewalls of equal length.

[0036] The ratio of the cage height of each enclosure in the at least one enclosure to the main dimension of the floor area may be at least 1.0.

[0037] A cage assembly may include at least one enclosure. Each enclosure may have: a floor defining a floor area having a principal dimension; and a cover having a bottom surface. The distance between the bottom surface of the cover and the floor may define the cage height. At least one sidewall may extend between the floor and the cover. The height h may be a function of the principal dimension Y of the floor according to the following formula: h ≥ (Y² – 6400) / 320, where h and Y are in millimeters.

[0038] One method may include placing animal test subjects within each enclosure of a cage assembly; and connecting the distal end of a cable to the animal test subject within each enclosure. At least 90% of the floor area of ​​the enclosure may be accessible to the animal test subject.

[0039] The method may further include connecting the proximal end of each cable to a rotary connector assembly. Each cable may have an operating portion having an operating length. Each operating length may be selected such that each test subject is not located within its respective enclosure, i.e., in a location where the spacing between the operating portions of the cables is within a threshold distance from the floor.

[0040] Animal subjects for testing can be mice.

[0041] The cage assembly may include a first enclosure and a second enclosure. A first mouse may be placed inside the first enclosure. A second mouse may be placed inside the second enclosure.

[0042] The first enclosure and the second enclosure may share a common sidewall that separates the floor areas of the first enclosure and the second enclosure. This common sidewall may define at least one opening between the first enclosure and the second enclosure. The at least one opening may allow communication between the first mouse and the second mouse.

[0043] The wires can be connected to the animal test subject via a treatment assembly that includes a transducer array.

[0044] The method may further include: using wires and treatment components to apply an electric field to an animal test subject within at least one enclosure.

[0045] Animal test subjects can have tumors, and the electric field can be a tumor treatment field.

[0046] The method may further include: inspecting or approaching the animal test subject through the cage assembly without removing the animal test subject from the cage assembly.

[0047] The method may further include: removing the animal test subject from the cage assembly; and autoclaving the floor, cover, and at least one sidewall of each enclosure.

[0048] Additional advantages of the invention will be set forth in part in the description which follows, and in part will be apparent from the description, or may be learned by practice of the invention. The advantages of the invention will be realized and achieved by means of the elements and combinations particularly pointed out in the appended claims. It will be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only, and do not limit the invention as claimed. Attached Figure Description

[0049] These and other features of the preferred embodiments of the invention will become more apparent from the detailed description herein with reference to the accompanying drawings, in which:

[0050] Figure 1 This is a system for experimental TTField treatment according to the embodiments disclosed herein.

[0051] Figure 2 They are test subjects with attached therapeutic components.

[0052] Figure 3 This is an exploded view of a cage system according to an embodiment of the present disclosure.

[0053] Figure 4 yes Figure 3 Front view of the cage system.

[0054] Figure 5 yes Figure 3 Side view of the cage system.

[0055] Figure 6 The test subjects were Figure 3 A schematic diagram of the interior of the cage system, approximating... Figure 5 The cross section taken from plane P.

[0056] Figure 7 It is shown Figure 3 A schematic diagram of measurements inside the enclosure of the cage system.

[0057] Figure 8 It is based on the embodiments disclosed herein and as follows Figure 1 An exploded view of the treatment components used together with the system.

[0058] Figure 9 It is based on the embodiments disclosed herein and as follows Figure 1 An exploded view of another treatment component used in conjunction with the system.

[0059] Figure 10A This is an exploded view of a contrast heater assembly according to an embodiment disclosed herein. Figure 10B yes Figure 10A A portion of the circuit board of the reference heater assembly.

[0060] Figure 11A This is an exploded view of another contrast heater assembly according to the embodiments disclosed herein. Figure 11B yes Figure 11A A portion of the circuit board of the reference heater assembly.

[0061] Figure 12A This is a partial exploded view of a cage according to an embodiment disclosed herein, showing the positioning of the rotary joint relative to the associated cage assembly. Figure 12B yes Figure 12A Detailed view of the rotary joint.

[0062] Figure 13 yes Figure 12B Side view of the circuit board of the rotary joint assembly.

[0063] Figure 14 yes Figure 13 A transparent view of the circuit board, showing the internal circuitry.

[0064] Figure 15 yes Figure 12B Exploded view of the rotary joint.

[0065] Figure 16 yes Figure 12B Side view of the rotary joint.

[0066] Figure 17 yes Figure 12B Bottom side view of the rotary joint.

[0067] Figure 18A yes Figure 12B A perspective view of the rotary joint. Figure 18B yes Figure 18A A detailed perspective view of a portion of a rotary joint, illustrating a torque sensor assembly with a pivot in a neutral position.

[0068] Figure 19 Is it like this? Figure 18B A perspective view of the rotary joint section, illustrating the pivot rotating from the neutral position.

[0069] Figure 20 This is a schematic diagram of a treatment component for providing treatment to organ tumors according to embodiments disclosed herein.

[0070] Figure 21 This is a schematic diagram of a treatment component for providing treatment to a subcutaneous tumor according to embodiments disclosed herein.

[0071] Figure 22 Is with Figure 1 A schematic diagram of a computing device used in conjunction with the system.

[0072] Figure 23 This is an exploded view of an embodiment of the rotary joint and its housing.

[0073] Figure 24 This is an exploded view of an exemplary treatment component.

[0074] Figure 25 Is it like this? Figure 24 A top view of an exemplary treatment component.

[0075] Figure 26 It is positioned on the test subject. Figure 24 A perspective view of the treatment components.

[0076] Figure 27 It is positioned on the test subject. Figure 24 A top view of the treatment components.

[0077] Figure 28 yes Figure 24 A top view of the treatment components, showing the communication between the electrodes.

[0078] Figure 29 yes Figure 24 A top view of the treatment components, showing alternating outline sections.

[0079] Figure 30 This is a side view of a collar according to an embodiment disclosed herein.

[0080] Figure 31 yes Figure 30 Side view of the collar.

[0081] Figure 32 yes Figure 30 A top view of the collar. Detailed Implementation

[0082] The invention will now be described more fully below with reference to the accompanying drawings, which illustrate some, but not all, embodiments of the invention. In fact, the invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements. Throughout, similar numerals refer to similar elements. It will be understood that the invention is not limited to the specific methods and protocols described and is therefore subject to variation. It will also be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention.

[0083] Benefiting from the teachings presented in the foregoing description and the associated drawings, those skilled in the art will conceive of many modifications and other embodiments of the invention set forth herein. Therefore, it will be understood that the invention is not limited to the specific embodiments disclosed, and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terminology is used herein, it is used only in a general and descriptive sense and not for limiting purposes.

[0084] As used herein, unless the context clearly indicates otherwise, the singular forms “a,” “an,” and “the” include plural indicators. For example, the term “electrode” can refer to one or more of such electrodes.

[0085] Unless otherwise expressly indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0086] As used herein, the terms “optional” or “optionally” mean that an event or situation described below may or may not occur, and the description includes instances where the event or situation occurs as well as instances where the event or situation does not occur.

[0087] As used herein, the term “at least one of…” is intended to be synonymous with “one or more of…”. For example, “at least one of A, B, and C” explicitly includes only A, only B, only C, and combinations of each.

[0088] A range may be expressed herein as from “about” a particular value and / or to “about” another particular value. When such a range is expressed, the other side includes from that one particular value and / or to that other particular value. Similarly, when a value is expressed as an approximation using the antecedent “about,” it will be understood that the particular value forms another aspect. It will be further understood that the endpoints of each range are both significantly related to and significantly independent of the other endpoint. Optionally, in some aspects, when an estimated value is approximated using the antecedent “about,” it is contemplated that values ​​up to 15%, up to 10%, up to 5%, or up to 1% higher or lower than the particular stated value can be included within the ranges of those aspects. Similarly, the use of “substantially” (e.g., “substantially parallel”) or “approximately” (e.g., “approximately plane”) should be understood to include embodiments in which the angle is within about ten degrees, or five degrees, or one degree.

[0089] As used in this article, the word “or” means any one of the components of a particular list, and also includes any combination of the components of that list.

[0090] It will be understood that, unless expressly stated otherwise, no method described herein is intended to be construed as requiring its steps to be performed in a particular order. Therefore, no inference is intended in any respect regarding the order unless a method claim actually describes the order in which the steps are to be followed, or unless the claims or description specifically state that the steps are limited to a particular order. This applies to any possible non-express basis of interpretation, including: logical questions concerning the arrangement of steps or the flow of operations; obvious meanings derived from grammatical organization or punctuation; and the number or type of aspects described in the description.

[0091] In the following description and claims, whenever the words “comprise” or “include” are used, it should be understood that the words “comprise” and “include” may optionally be replaced with the words “consistent with” or “comprise with” to form another embodiment.

[0092] The following description provides specific details to provide a thorough understanding. However, those skilled in the art will understand that the equipment, system, and associated methods can be implemented and used without employing these specific details. In fact, the equipment, system, and associated methods can be put into practice by modifying the illustrated equipment, system, and associated methods, and can be used in conjunction with any other equipment and technologies routinely used in industry.

[0093] TTFields (also referred to herein as alternating electric fields) have been established as an anti-mitotic cancer therapy because they interfere with proper microtubule assembly during metaphase and ultimately destroy cells during telophase and cytokinesis. Efficacy increases with increasing field strength, and the optimal frequency is cancer cell line-dependent, with 200 kHz being the frequency at which TTField-induced inhibition of glioma cell growth is highest. For cancer therapy, non-invasive devices have been developed utilizing capacitively coupled transducers placed directly in the skin region near the tumor. For patients with glioblastoma multiforme (GBM) (the most common primary, malignant brain tumor in humans), the system used to deliver TTField therapy is called the OPTUNE™ system (Novocure Ltd.).

[0094] Because the action of TTfield is directional, cells dividing parallel to the field are more affected than those dividing in other directions. And because cells divide in all directions, TTfield is typically delivered via two pairs of transducer arrays that generate a vertical field within the treated tumor. More specifically, for the OPTUNE system, one pair of electrodes is positioned on the left and right sides of the tumor (LR), and another pair is positioned on the anterior and posterior sides of the tumor (AP). The cyclic field between these two directions (i.e., LR and AP) ensures targeting to the maximum extent possible with respect to cell orientation.

[0095] Although TTField has been approved for use in certain patients, a system is still needed that allows for reliable, consistent, and safe testing of TTField in animal subjects. In studies involving small animals (e.g., mice) in which electrical components are attached, the animals frequently chew or otherwise damage the components. Additionally, when animals are tethered with cables, they often cause the cables to twist. When too much slack is provided in such cables, the animal can easily roll over, leading to damage or mispositioning of the electrical components. When insufficient slack is provided in such cables, the animal's movement is too restricted. Furthermore, attaching the electrical components to the animal can be difficult without significant adjustments and repositioning.

[0096] In all aspects and reference Figure 1 This document discloses a system 10 for providing TTField to test subjects 12 (e.g., animal test subjects, such as mice). System 10 may include one or more cage components 100 to receive and accommodate one or more test subjects. Some test subjects in experimental group 14 may be equipped with TTField treatment components 200, 200', 200"..." Figure 8 , Figure 9 and Figure 24The TTField treatment assembly may include a transducer array for delivering treatment to test subjects. Other test subjects in control group 16 may be equipped with control heater treatment assemblies 400, 400' (…). Figure 10A-11B The control heater treatment assembly is configured to provide the same weight and heat as the TTField treatment assemblies 200, 200', 200"). Multiple TTField treatment assemblies 200, 200', 200" can be communicatively coupled to the TTField generator 18. Optionally, the TTField generator may be a generator provided as part of the INOVITRO Laboratory Research System (NOVOCURE GMBH). Similarly, multiple control heater treatment assemblies 400 can be communicatively coupled to the same or separate TTField generator 18 (or other generators capable of initiating heat via the control heater treatment assembly, as further disclosed herein). A computer 1001 can be communicatively coupled to the TTField generator 18. The computer 1001 can control the output of the multiple TTField generators 18, as well as log data from the TTField generator 18, treatment assemblies 200, 200', 200", control heater treatment assembly 400, and / or test subject 12.

[0097] TTField treatment components 200, 200', 200” and control heater treatment component 400 can be connected via their respective cables 204 ( Figure 8 The test subject communicates with the TTField generator 18. To allow the test subject to move freely within the cage assembly 100 without tangling with the cable 204, the cable 204 can extend and be connected to a rotary joint 300 (which may also be interchangeably referred to herein as the rotary joint assembly 300). The rotary joint 300 can then be connected to a second cable 20, which extends and is connected to the TTField generator 18. Thus, as further disclosed herein, the rotary joint 300 enables electrical communication from the TTField generator 18, through the second cable 20, through the rotary joint assembly 300 to the cable 204 for communication with the treatment components 200, 200', 200" while preventing tangling of the cable 204.

[0098] Cage assembly

[0099] refer to Figure 3-5The cage assembly 100 may include a body 102. The body 102 may include a floor 104 defining a floor area with a primary dimension. Optionally, the floor 104 may be rectangular or generally rectangular and have corners 16. Optionally, the corners 16 may be rounded. The corners may have a radius of, for example, approximately 17 mm. The primary dimension may be the largest diagonal between the corners 106 of the cage. The body 102 may further include one or more sidewalls 108. For example, the body 102 may include a front sidewall 108A, an opposing rear sidewall 108B, and a pair of opposing sidewalls 108C extending between the respective edges of the front sidewall 108A and the rear sidewall 108B. The intersection between the respective sidewalls may define a rounded corner 110. Optionally, the sidewalls 108 may converge in a direction toward the floor 104 (i.e., extend inwardly at a downward direction) to provide a release angle for manufacturing via injection molding. Optionally, the floor may include bedding material commonly used in conventional animal cages. The bedding material can be, for example, sawdust. Food pellets can be placed on the floor of the enclosure for foraging. A standard water bottle can be attached to the cage for the subjects to replenish their water supply. Optionally, the cage may include an opening in the side wall of each enclosure to receive a dispensing portion of the standard water bottle.

[0100] In the exemplary aspect, and as Figure 3 As shown, sidewall 108 may define a plurality of orifices 130 for ventilation. One or more filters 132 may optionally cover the plurality of orifices in each sidewall 108. Frame 134 may extend around the periphery of filter 132 and receive fasteners (e.g., nuts 136 and bolts 138) for attachment to the body 102 of cage assembly 100. In this way, the cage assembly is sealed such that all or substantially all of the ventilation to each enclosure travels through at least one filter before entering the ventilation opening. Optionally, as Figure 3 As shown, a single filter 132 may cover multiple openings 130 (optionally, all openings) of the sidewall 108. The filter may be removable, autoclaved, and replaceable. The filter minimizes the penetration of infectious materials and organisms while allowing for rapid air exchange. It is envisioned that a mesh, screen, grid, breathable membrane, or other permeable structure may be positioned between the multiple openings 130 and the filter 132 within the cage to prevent test subjects from chewing on the filter.

[0101] like Figure 3As shown, door 112 is pivotally connected to body portion 102 via a pair of hinges 114. In use, door 112 is movable about and between: (1) a closed position, wherein door 112 cooperates with sidewalls to provide enclosure(s); and (2) an open position, wherein door pivots away from the interior of cage assembly to provide one or more openings through which access to the interior of cage assembly is possible.

[0102] The cover 120 may extend across the top of the body 102. The cover 120 may be releasably attached to the body 102 via a latch 122. The latch 122 may be pivotally attached to the body 102 via a hinge 124. A latch 126, pivotable about a hinge 128, may be attached to the door 112. The latch 126 may releasably engage a latching element on the top of the cover 120 for holding the door 112 in a closed position. Optionally, the cover 120 may include one or more rotary joint housings 180 configured to receive at least a portion of a rotary joint, as further disclosed herein.

[0103] The partition 140 may define a common sidewall dividing the interior of the cage into a first enclosure 142 and a second enclosure 144. The partition 140 may optionally be removable. The body may optionally define a slot into which the partition 140 can be inserted. The partition 140 may define an opening 146 (optionally, multiple openings) between the first enclosure 142 and the second enclosure 144 to allow the respective test subjects 12 in each of the first and second enclosures to interact with each other (e.g., through acoustic interaction, through odor, through body warmth, etc.). Thus, the first enclosure 142 and the second enclosure 144 may each have a respective sidewall (or sidewall portion) defined by a front sidewall 108A, a door 112, a rear sidewall 108B, a sidewall 108C extending between the front and rear sidewalls, and the partition 140. In these examples, it is envisioned that the floor area within each enclosure can have its own principal dimension, which can be equal to the maximum diagonal between the corners of the enclosure.

[0104] The sidewalls (e.g., body 104 and partition 140) and lid may optionally comprise polycarbonate and may optionally be autoclaved. Parts of the cage, such as, for example, body 102 and lid 120, may be transparent so that the test subject can be observed when the cage is closed.

[0105] refer to Figure 6The shielding subassembly 150 can extend inwardly from the partition 140 into each of the first enclosure 142 and the second enclosure 144. Within each enclosure, the shielding subassembly 150 may include an arched top, a pair of parallel walls extending vertically downward from the arched top, and optionally a floor covering extending between the bottom edges of the side walls of the shielding subassembly. Within each enclosure, the shielding subassembly 150 can extend from the partition 140 by a selected distance D. Optionally, the distance D can be approximately 4-5 cm. It is contemplated that the distance D can be selected such that the cable will not restrict the interaction between the test subject and the test subject in relation to the enclosure. For example, the cable can be tied to the back of the test subject away from the head of the test subject by a selected distance d. In this way, the test subject will not chew the cable. This selected distance also allows the test subject to enter the shielding subassembly before the cable reaches it. Furthermore, the cable can be flexible enough to bend upon contact with the shielding subassembly. A selectable distance can be chosen for the shielding sub-assembly 150 to protrude from the partition 140 such that when the cable is fully taut relative to the shielding sub-assembly, the test subject (e.g., at least the nose and / or face of the test subject) can reach at least the plane defined by the partition 140. As further disclosed herein, the cable length can be a function of the dimensions of the enclosure. Therefore, the selectable distance D for the shielding sub-assembly 150 to protrude from the partition 140 can be a function of the cable length and the height and width dimensions of the enclosure.

[0106] The cover 120 for the first and second enclosures can be integrally constructed as a cover assembly 178. Optionally, the cover assembly 178 may include a rotary joint housing 180. In these aspects, the cover assembly 178 may further include rotary joints 300, as further disclosed herein, wherein these rotary joints are positioned within their respective rotary joint housings 180. The cover assembly 178 may include a first opening and a second opening between the first and second enclosures and their respective rotary joints 300. The first and second openings may provide communication to allow cables of the treatment assembly to be coupled to their respective rotary joints. Each rotary joint may be in a covering relationship with each of the first and second openings. Depending on various aspects, each rotary joint may extend through its respective opening in the cover assembly and at least partially extend into its respective enclosure to receive its respective cable. In a further embodiment, each cable may extend through each of the first and second openings to be coupled to its respective rotary joint.

[0107] refer to Figure 4-7The distance between cover 120 and floor 104 can define the cage height. To prevent the test subject from having sufficient slack in the cable 204 to flip over or become entangled with the cable, the cage assembly can have a selected cage height h, which is a function of the length R1 and width R2 of each enclosure. For example, the cable 204 can have a selected length to prevent providing sufficient slack for the test subject to coil the cable around its body. Depending on some optional aspects, the dimensions of each enclosure 142, 144 can be selected such that the test subject can approach the corner of the cage, but when the test subject is positioned directly below the cable attachment to the swivel joint 300, the cable does not have sufficient slack to hang from the back of the test subject or extend downwards to touch the floor of the cage. To maximize the usable area for a given cage height, the cable can extend directly above the center of the floor space of each enclosure. Therefore, the cage height can be selected as a function of the main dimensions of the cage floor (for a given enclosure) and the height of the test subject. For example, the height can be selected based on the following formula:

[0108] h ≥ (R1 2 + R2 2 – 16a 2 ) / 16a

[0109] Where h is the height of the cage, R1 is the length of the enclosure, R2 is the width of the enclosure, and a is the height of the animal.

[0110] Therefore, according to the following formula, the height can be a function of the main dimension Y of the cage floor:

[0111] h ≥ (Y 2 – 16a 2 ) / 16a.

[0112] A typical test subject, a mouse, can have a height of 20 mm (a). Therefore, in some examples, the cage height h in millimeters can be a function of the main dimension Y (in millimeters) of the cage floor, according to the following formula:

[0113] h ≥ (Y 2 – 6400) / 320 [mm].

[0114] More generally, the height of the cage can be chosen as the main dimension of the cage floor (of each enclosure) multiplied by a factor. Depending on some aspects, the height of the cage can be at least 0.5 times, at least 0.6 times, at least 0.7 times, at least 0.8 times, or at least, or at least 1.1 times, or at least 1.2 times the main dimension of the cage floor.

[0115] In some exemplary embodiments, the floor of the cage assembly may have a length of approximately 315 mm and a width of approximately 185 mm. Therefore, with partitions dividing the floor length, each enclosure may have a floor with a long side of 185 mm and a short side of 157 mm. Thus, the floor area of ​​each enclosure may have a principal dimension of 242 cm (equal to the maximum diagonal between the corners of the enclosure). Therefore, it is contemplated that the cage height may have a minimum height of at least 163 mm, providing a height approximately 0.7 times the principal dimension of the cage floor. Depending on various aspects, the floor area of ​​each enclosure may have the following minimum principal dimensions: at least 160 mm, between approximately 160 mm and approximately 200 mm, between approximately 200 mm and approximately 250 mm, between approximately 250 mm and approximately 300 mm, between approximately 300 mm and approximately 400 mm, or more than 400 mm. In some embodiments, the cage height may be approximately 260 mm. In a further embodiment, the height of the cage may be at least 60 mm, at least 105 mm, at least 175 mm, at least 261 mm, or at least 480 mm.

[0116] It is envisioned that the above formula for selecting the cage height is not absolute, because the cable has a certain amount of stiffness (i.e., the cable's flexibility is limited), thereby limiting the cable's ability to reach the cage floor. Therefore, it is envisioned that the cage height can be less than the minimum height specified in the above formula, while still providing sufficient cage height to prevent the test subject from becoming entangled.

[0117] Optionally, the cage may include a feeder (e.g., a food tray or food dispenser). The feeder may optionally be attached to the partition 140 or other sidewall so that the feeder remains suspended.

[0118] Treatment components

[0119] refer to Figure 8The treatment component 200 can be configured to provide a TTField to an organ tumor. The treatment component 200 may include a flexible circuit board 202 including a connector end 206 and one or more lead ends 208 at the ends of respective electrical leads 205 opposite to the connector end 206. The electrical leads 205 may be provided as part of a cable 204. Optionally, the flexible circuit board 202 may be configured to be coupled to a rotary joint 300. The cable 204 may be elongated and sufficiently flexible to allow a certain amount of twisting without requiring a rotary joint. The connector end 206 may optionally be a USB-C connector (e.g., a male USB-C connector). It is contemplated that using the flexible circuit board 202 as disclosed herein allows multiple electrical leads 205 to be provided as part of a unified structure, thereby avoiding cable or wire tangling and minimizing the space occupied by the electrical leads. Therefore, in some aspects, the cable 204 may be defined by a portion of the flexible circuit board 202.

[0120] In such Figure 8 In the pre-use configuration shown, the lead ends 208 may be spaced apart along the longitudinal dimension 201 of the treatment assembly 200. Optionally, the lead ends 208 may be arranged in one or more longitudinally extending rows. For example, the lead ends 208 may be arranged in two rows of four lead ends each, wherein these two rows extend along the longitudinal dimension 201. As another example, the one or more longitudinally extending rows may comprise a single row of lead ends 208. However, it is contemplated that any desired arrangement of the lead ends may be used. The cable 204 may extend perpendicularly or substantially perpendicularly to the longitudinal dimension 201.

[0121] Each lead end 208 may be configured to engage or be coupled to a respective plate 210. Thus, the treatment assembly 200 may include a plurality of electrodes, each electrode including a lead end 208 that contacts or is otherwise coupled to a respective plate 210. In some optional embodiments, at least one (optionally, each) plate 210 may be a ceramic plate. In other embodiments (such as those in which the treatment assembly 200 serves as a control heating device), it is contemplated that at least one (optionally, each) plate may be a glass plate. In a further aspect, other types of electrodes are contemplated, such as, for example, electrodes formed of metal or other conductive materials.

[0122] The treatment component 200 may include an inner layer 212 having an outer surface 214 and an inner surface 216. The inner layer 212 may include a biocompatible, breathable adhesive, such as, for example, polyurethane. In some embodiments, the inner layer may include a VANCIVE MED 9598A polyurethane membrane with an acrylic adhesive. The inner layer 212 may define a plurality of openings 218 through the inner layer for receiving respective plates 210. The openings 218 may be spaced apart longitudinally along the inner layer. Although depicted as receiving individual plates, it is contemplated that each opening may optionally receive multiple plates (e.g., two plates).

[0123] Plate 210 may have an upper surface 220 and a lower surface 222. The upper surface may be disposed abutting against the electrical lead 208. Hydrogel layer 224 may be disposed abutting against the lower surface 222 of each of the plates 210. Hydrogel layer 224 may optionally cover at least two adjacent plates 210. Optionally, hydrogel layer 224 may cover the adjacent portion of the lower surface 222 of plate 210 and the inner surface of inner layer 212. In some optional aspects, hydrogel layer 224 may be approximately 0.6 mm thick. Hydrogel 224 may include, for example, AG625 sensing gel manufactured by AXELGAARD.

[0124] Cover layer 230 may be attached to the outer surface 214 of inner layer 212. Cover layer 230 may cover multiple electrical leads 208 of flexible circuit board 202. Cover layer 230 may include one or more tab portions extending beyond the periphery of inner layer 212. For example, cover layer 230 may include two opposing tab portions 232 that are complementary to each other when the cover layer defines a circumferential ring (e.g., when wound around the torso of a test subject, as further disclosed herein). Optionally, the tab portions 232 may be approximately half the width of the cover layer at their intersection with the body portion of the cover layer. When the cover layer is wound around the torso of a test subject, the tab portions 232 may extend over each other to attach to the respective portions of the cover layer at their opposing ends.

[0125] Depending on some optional aspects, the cover layer may have an inner surface comprising a biocompatible nonwoven adhesive. The nonwoven adhesive may optionally be elastic along the longitudinal dimension 201. In some embodiments, the cover layer may comprise medical nonwoven tape, product number 1776, manufactured by 3M.

[0126] Release layer 250 may contact and cover the underside of the biocompatible, breathable polyurethane adhesive on the inner surface 216 of inner layer 212, and the underside of hydrogel layer 224. The release layer may protect the adhesive before the therapeutic component is attached to the test subject. Release layer 250 may have a shape complementary to that of cover layer 230. Release layer may include separate tabs 252 configured to cover tab portions 232 of cover layer 230.

[0127] The treatment component may include at least one temperature sensor 260 (not shown, but the temperature sensor 260 may have a corresponding...) Figure 10B and Figure 11B The at least one temperature sensor (the location of temperature sensors 414 and 414') may be, for example, a thermistor or thermocouple. The at least one temperature sensor may optionally be part of the flexible circuit board 202. The at least one temperature sensor may include multiple temperature sensors. For example, temperature sensors may be positioned on the flexible circuit board 202, near each lead end 208. The multiple temperature sensors 260 may provide feedback to prevent the treatment assembly from overheating or causing burns to the test subject. For example, based on a temperature reading from temperature sensor 260 exceeding a threshold (e.g., 40°C), the TTField generator 18 may adjust or stop the sensing of the TTField at one or more electrodes. Additionally, the system 10 may receive feedback from temperature sensor 260 to maintain a consistent temperature in the control heater treatment assembly further disclosed herein. Optionally, the temperature sensors may be positioned within respective holes in their respective plates to measure the temperature between the plate and the hydrogel. In a further aspect, the temperature sensors may be positioned on the side of the respective plate opposite the hydrogel, thereby avoiding the need to form holes in the plate (and potentially making the plate undesirably fragile). In a further embodiment, the temperature sensor may be positioned on one side of the respective plate at a portion of the hydrogel (e.g., within three millimeters of the plate edge). For example, as... Figure 10A As shown, pairs of plates 412 can share a single hydrogel layer 410, and each temperature sensor can be positioned between its respective pair of plates.

[0128] The portion of the treatment component that supports the test subject (e.g., excluding the weight of the cables) may optionally weigh less than about 10 percent of the test subject's body weight. For example, for a typical mouse, the portion of the treatment component that supports the test subject may weigh less than about 2.5 grams.

[0129] The treatment component can be flexible enough to conform to a portion of the torso of the test subject 12. Optionally, in such a way... Figure 8In the pre-use configuration shown, the treatment component has a length along longitudinal dimension 201 sufficient to extend around the torso circumference of the test subject (both when positioned on the animal and during use). Optionally, the treatment component may be pre-formed into a three-dimensional shape configured to complement the torso shape of the test subject 12.

[0130] Optionally, the kit may include multiple treatment components 200, which have different lengths along the longitudinal dimension 201 (in the pre-use configuration). In this way, the test subject can be fitted with treatment components of appropriate size (depending on the animal's girth / circumference). For example, the appropriately sized treatment components may be tightly wrapped around the test subject's torso girth. Optionally, additional covering material (which may be, for example, the same material as the outer layer) may be provided to reinforce attachment to the test subject and seal the edges of the adhesive material against dirt and debris that might hinder good contact.

[0131] refer to Figure 9 The treatment component 200' can be configured for treating subcutaneous tumors. The treatment component 200' can have a configuration generally similar to that of the treatment component 200, having an inner layer 212' defining an opening 218' in which a receiving plate 210' is received. Optionally, it is contemplated that a hydrogel 224' can be received within the opening 218'. The flexible circuit board 202' may include cables 204' configured to connect to a rotary joint 300. Figure 1 The connector end 206' and one or more lead ends 208' at the lead end opposite to the connector end 206'. The lead ends 208' can be configured to be coupled to their respective plates 210'. The outer layer 230' can be attached to the respective upper surfaces of the inner layer and the lead ends. The release layer 250', including separate tabs 252', can be attached to the bottom side of the inner layer 212' and the hydrogel 224'. The outer layer 230' and the release layer 250' can be constructed similarly to the outer layer 230 and the release layer 250.

[0132] The inner layer 212', circuit board 202', and cover layer 230' can cooperate to define a through-hole 270' that extends through the thickness of the treatment assembly 200' (except for the inner release layer (when present)) and is configured to receive a subcutaneous tumor. Optionally, the through-hole 270' can have a diameter between 10 mm and 15 mm. Optionally, the through-hole 270' can have a maximum diameter of approximately 15 mm. A cap 272' defines a receiver 274' configured to receive an outwardly extending portion of a subcutaneous tumor therein, the cap extending across the through-hole 270'. The cap 272' can be attached to the cover layer 230. For example, the cap 272' can define a radially extending peripheral edge 276'. An adhesive ring 278' can engage the flange 276' and the outer layer 230' to secure the cap 272' to the outer layer. Cap 272' prevents dirt and debris (e.g., sawdust floor coverings) from entering the hole and prevents contact between the treatment component and the test subject.

[0133] In an exemplary aspect, the opening 218' received therein in the plate 210' may have a predetermined relationship with respect to the through hole 270'. Optionally, in these aspects, and as... Figure 9 and Figure 11A As shown, the opening 218' can be circumferentially spaced around the periphery of the through hole 270' (and thus around the subcutaneous tumor when the tumor extends through the through hole 270').

[0134] According to some aspects, the kit may include a plurality of treatment components 200', which have through holes 270' of different diameters and corresponding caps 272' of different sizes. The plurality of treatment components 200' with through holes of different diameters may optionally have correspondingly different spacing between the lead end 208' and the plate 210'. In this way, the test subject 12 may be fitted with a treatment component 200' appropriately sized for its subcutaneous tumor. Optionally, the kit may further include a plurality of caps (optionally, of the same size and / or of different sizes) such that these caps can be replaced during treatment.

[0135] The lengths of cables 204 and 204' can be selected based on the enclosure dimensions to prevent the test object 12 from being coiled or tangled with the cable. A certain amount of slack can be attached to the back of the test object to reduce the amount of free length of the cable. Therefore, the cable can have an operating portion not attached to the test object, wherein this operating portion can limit the operating length of the cable. According to some aspects, the operating length of the cable can be selected such that when the test object is directly below the rotary joint, the cable does not have sufficient length to suspend from the back of the test object and touch the floor. Therefore, it can be understood that the maximum operating length of the cable can be approximately estimated as the height of the cage (or the height at which the cable is attached to the rotary joint) plus twice the height of the test object. In a further aspect, the cable length can be selected such that the cable does not have sufficient slack to suspend from the back of the test object to a threshold distance t from the floor of the cage. Figure 6 Therefore, the maximum operating length of the cable can be approximately estimated as the height of the cable attached to the swivel joint from the floor plus twice the height of the test object, minus twice the threshold distance. Optionally, the threshold distance can be 0 mm, 1 mm, 2 mm, 4 mm, 6 mm, 10 mm, or more. In some aspects, the threshold distance can range from about 1 mm to about 10 mm, or from about 2 mm to about 6 mm. Further envisioning this, due to the limited flexibility of the cable, the length of the operating portion of the cable can be slightly greater than twice the height of the test object plus the height of the cable, without the cable reaching the floor of the cage.

[0136] To construct the therapeutic assembly 200, each of the plurality of plates 210 may be positioned within an opening in the inner layer of the therapeutic assembly. For example, in some embodiments, a pair of plates may be positioned within each opening. Alternatively, a single plate may be positioned within each opening. Each of the plurality of guide ends may be positioned to contact a respective plate of the plurality of plates. As previously stated, when coupled to the plates disclosed herein, lead ends form respective electrodes. A capping layer may be attached to the outer surface of the inner layer such that the capping layer covers the plurality of electrodes. A hydrogel layer may be applied to the lower surface of each of the plurality of plates. In some aspects, a pair of plates positioned within a common opening in the inner layer may also share a single hydrogel layer. Optionally, the hydrogel may be applied to adjacent portions of the inner surface of the inner layer.

[0137] refer to Figure 24-29 In a further aspect, it is envisioned that the treatment component 200” can be configured to be at least partially positioned on the head of the test subject. For example, the head covering portion 9a of the treatment component 200” can be attached to at least a portion of the mouse's head, such as... Figure 26As shown in the diagram. The head cover portion 9a of the treatment component 200” may include a head wearable layer 6 configured to extend over a portion of the test subject’s head and be attached to the test subject by an adhesive positioned on one or more inner surfaces of the head wearable layer 6. The treatment component 200” may further include a torso cover portion 9b configured to be placed on the test subject’s body (e.g., torso) (e.g., wrapped around the test subject’s body / torso, as further disclosed herein). The torso cover portion 9b of the treatment component 200” may include an inner adhesive wearable layer 1 configured to engage the test subject’s body (optionally, skin). The flexible circuit board 5 may include a plurality of lead ends. One end 8 of the flexible circuit board may be connected to the TTField generator 18 ( Figure 1 Communication. The inner adhesive patch 4 can be attached to the skin-contact side of the adhesive portion 6, and a portion of the flexible circuit board 5 is positioned therebetween. The outer wearable layer 7 can be attached to the outside of the torso covering portion 9b of the treatment assembly.

[0138] A plate (e.g., a ceramic plate) 3 can be attached to the lead end of a flexible circuit board. Hydrogel 2 can be positioned beneath the ceramic plate to adhere to the patient's skin. An inner adhesive wearable layer 1 of the torso covering portion 9b can define at least one opening (optionally, multiple openings) for receiving the corresponding portion of the hydrogel 2.

[0139] The flexible circuit board 5 may include a plurality of leads (and thus a plurality of electrodes 602) configured to be positioned on the head of a test subject, and one or more leads (e.g., two leads) (and thus a plurality of electrodes 604) configured to be positioned on the body (e.g., torso) of the test subject. In an exemplary aspect, the plurality of leads (for positioning on the head) are configured to be located under the head wearable layer 6, and the one or more leads (for positioning on the torso) are configured to be located under the outer wearable layer 7 of the torso covering portion 9b, wherein each lead covers its respective ceramic plate 3 and hydrogel portion 2. Optionally, the plurality of lead ends configured to be positioned on the head of the test subject may include: a first set of lead ends (e.g., the three lead ends corresponding to electrode 602a), configured to be positioned on a first side of the test subject's head (relative to the midline surface 606 that divides the test subject into left and right sides); and a second set of lead ends (e.g., the three lead ends corresponding to electrode 602b), configured to be positioned on a second opposite side of the test subject's head (relative to the midline surface). The one or more lead ends configured to be positioned on the body (e.g., torso) of the test subject may include: a first lead end (corresponding to electrode 604a), positioned relative to the midline surface on a first side of the test subject's body; and a second lead end (corresponding to electrode 604b), positioned relative to the midline surface on a second side of the test subject's body. Reference Figure 28 The envisioned configuration is that a first lead end positioned on a first side of the body can cooperate with a second set of lead ends positioned on a second side of the test subject's head to provide a TTField, and a second lead end positioned on a second side of the body (torso) can cooperate with a first set of lead ends positioned on a first side of the test subject's head to provide a TTField. TTFields can be provided alternately to provide or facilitate the crossing of TTFields.

[0140] In an exemplary aspect, the flexible circuit board 5 may include wavy (e.g., tortuous), serpentine, wave-like, or zigzag portions (generally referred to as "alternating profile portions" 700) configured to facilitate flexibility to allow the test object to move its neck. In use, it is contemplated that the alternating profile of this portion of the flexible circuit board 5 can provide a reduced initial length (to avoid unnecessary slack in the cable) while also allowing straightening to increase length and accommodate movement of the test object (e.g., neck extension, twisting, and rotation). In these aspects, and as... Figure 24-25 and Figure 27-29 As shown, the alternating contour portion can be positioned between the head cover portion 9a and the torso cover portion 9b. Further envisioning is that the alternating contour portion of the flexible circuit board 5 can be positioned between the plurality of lead ends (for positioning on the head) and the at least one lead end (for positioning on the torso).

[0141] Exemplary, non-limiting dimensions of the treatment component 200” are shown in Figure 29 The measurement is provided in millimeters.

[0142] It is envisioned that the material and properties of the inner adhesive wearable layer 1 of the torso covering portion 9b may be the same as or similar to the material and properties of the covering layers 212, 212' disclosed herein with respect to treatment components 200, 200'. Similarly, it is envisioned that the material and properties of the hydrogel 2 of treatment component 200" may be the same as or similar to the material and properties of the hydrogels 224, 224' disclosed herein with respect to treatment components 200, 200'. It is further envisioned that the material and properties of the plate 3 of treatment component 200" may be the same as or similar to the material and properties of the plates 210, 210' disclosed herein with respect to treatment components 200, 200'. It is further envisioned that the material and properties of the flexible circuit board 5 may be the same as or similar to the material and properties of the flexible circuit boards 202, 202' disclosed herein with respect to treatment components 200, 200'. Furthermore, it is envisioned that the materials and properties of the head wearable layer 6 and the outer wearable layer 7 of the treatment component 200” may be the same as or similar to the materials and properties of the covering / outer layers 230, 230’ disclosed herein with respect to the treatment components 200, 200’.

[0143] refer to Figure 10A-11B The control heater treatment component 400 can be coupled to a control test subject and can be configured to mimic many or all or substantially all aspects of the treatment component 200. Similarly, the control heater treatment component 400' can be configured to mimic all or substantially all aspects of the treatment component 200' and can have a similar configuration and operation as described for the control heater treatment component 400. Likewise, the control heater treatment component can be configured to mimic all or substantially all aspects of the treatment component 200' and can have a similar configuration and operation as described for the control heater treatment component 400. For example, the control heater treatment component 400 can be configured to generate heat to maintain a similar temperature to the skin of the control test subject, thereby limiting differences between aspects of the control and experimental groups. As another example, the control heater treatment component 400 can be configured to have a weight substantially or substantially the same as that of the treatment component capable of generating TTField, as further disclosed herein.

[0144] The reference heater assembly 400 may include a flexible circuit board 402. The flexible circuit board 402 may include a cable 404 and a connector end 406 configured to connect to a rotary joint 300. The flexible circuit board may include a plurality of resistive heaters positioned in locations corresponding to the positions of electrodes in the treatment assembly 200. For example, the flexible circuit board 402 may include eight zones 410 (e.g., four zones 410 in two rows) in which electrodes will be positioned in corresponding treatment assemblies. More generally, the flexible circuit board 402 may have any desired number of zones, each zone corresponding to the position of an electrode in a corresponding treatment assembly. Optionally, each zone 410 may include two resistive heaters 412 (in... Figure 10A The component shown is schematically represented as a single unit separate from circuit board 402 and is shown in detail as a component of circuit board 402 in Figure 11. Temperature sensors 414 may be disposed in each zone 410, optionally at the center of each zone 410, and equally spaced between heaters 412. Heaters 412 may optionally include glass plates.

[0145] Optionally, the control heater assembly 400 may include an inner layer 420 defining a plurality of through-holes through which the heater 412 can be positioned. Optionally, the control heater assembly 400 may include a cover layer 430 extending across the upper side of the flexible circuit board. A release liner 440 may be releasably attached to the lower surface of the inner layer. The inner layer 420 and the cover layer 430 may include the same material and the same geometry of the corresponding treatment assembly in order to feel similar to the test subject. Optionally, a hydrogel layer 416 may cover the lower side of the flexible circuit board 402. Similarly, the control heater assembly 400' may have a structure corresponding to the structure of the treatment assembly 200', having a flexible circuit board 402', an inner layer 420', a release liner 440', a cover liner 430', a cap 450', and an adhesive ring 460'. Similarly, a control heater assembly simulating the shape, weight, heat, and other perceptual experiences of the treatment assembly 200' is further envisioned.

[0146] The control heater assembly 400 can be coupled to the TTField generator 18 via a rotary joint 300. The rotary joint 300 can control the output of the resistance heater 412 based on feedback from the temperature sensor 260. In some embodiments, the control heater assembly 400 can maintain a set temperature (e.g., 38.5 or 39 degrees Celsius) to mimic the temperature reached by the corresponding treatment components 200, 200', 200" as a byproduct of providing TTField. In a further embodiment, the control heater assembly 400 can be selectively controlled to maintain a temperature matching that of the treatment components on the corresponding test subjects receiving TTField treatment.

[0147] The control heater assemblies 400 and 400' can further have a weight similar to that of their respective treatment assemblies 200, 200', and 200" . Therefore, the control heater assemblies can produce the same perceptual experience in the test subjects. In this way, the effects of TTField on tumor development can be isolated from other aspects of the test procedure.

[0148] In an exemplary aspect, a kit may be provided that has both control heater assemblies 400, 400' and treatment assemblies 200, 200', 200"". In these aspects, it is envisioned that each treatment assembly provided in the kit may have a corresponding / corresponding control heater assembly located within the same kit, thereby maximizing consistency between the control group and the experimental / treatment group.

[0149] It is envisioned that, in addition to providing TTField, the embodiments disclosed herein can also be used to provide other currents, fields, and heat to different body parts of the test subject.

[0150] In some optional aspects, the wide treatment assembly (and corresponding control heater assembly) for test subjects with wide torsos may have a length of approximately 200 mm to approximately 250 mm (relative to longitudinal axis 201), a width of approximately 100 mm to approximately 130 mm (optionally, 110 mm to 115 mm), and a thickness of approximately 1.6 mm to approximately 1.7 mm. In some optional aspects, the narrow treatment assembly (and corresponding control heater assembly) for test subjects with narrow torsos may have a length of approximately 200 mm to approximately 250 mm, a width of approximately 80 mm to approximately 115 mm (optionally, 100 mm to 110 mm), and a thickness of approximately 1.6 mm to approximately 1.7 mm. In a further optional aspect, the treatment assembly (and corresponding control heater assembly) for subcutaneous tumors may have a length of approximately 290 mm to approximately 330 mm, a width of approximately 65 mm to approximately 95 mm, and a thickness of approximately 1.6 mm to approximately 1.7 mm.

[0151] Ring

[0152] The envisioned scenario is that test subjects will tend to chew or bite treatment components 200, 200', 200” or control heater treatment components 400, 400'. (Reference) Figure 30-32To prevent this behavior, it is envisioned that the collar 500 may be annular. Optionally, the collar 500 may have two opposing ends joined together to form a ring shape. For example, the collar 500 may include a protrusion 502 positioned at a first end 506, the protrusion being configured to receive into one or more holes 504 in the opposing second end 508. The protrusion 502 may have an enlarged distal end with a diameter larger than the diameter of the one or more holes 504, such that once inserted into the hole, it will not inadvertently fall out (due to the engagement between these surfaces of the protrusion and the portion of the second end defining the hole). It is envisioned that the one or more holes 504 may include a plurality of holes 504 spaced apart circumferentially around the collar 500, such that the collar may have a selectable operating diameter depending on the hole into which the protrusion 502 is inserted, so that the collar can be adapted to test objects defined by different sizes.

[0153] Optionally, the collar 500 may have an inner surface 510, which is irregular, serrated, or toothed. Optionally, the collar 500 may have an outer surface 512, which is axially tapered. The collar 500 may be oriented such that the outer surface tapers away from the head of the test subject.

[0154] It is envisioned that both excessive weight of the collar and sound reflections would shorten the lifespan of the test subject. Therefore, in some aspects, the collar 500 may define a plurality of holes 514, which may optionally extend axially through the collar (through the thickness of the collar). The holes 514 can reduce the amount of material, and thus reduce the weight of the collar, and minimize sound reflections that would cause stress on the test subject.

[0155] The collar 500 may optionally be flexible. Optionally, the collar may comprise a polymer, such as, for example, silicone.

[0156] Rotary joint assembly

[0157] Refer to Figure 12 and Figure 15 The rotary joint 300 can be mounted to a cover 120 within the rotary joint housing 180. The rotary joint housing 180 may include a sidewall 182, which is part of the cover 120. The rotary joint housing 180 may receive a removable inner circumferential insert 184. The rotary joint housing may further include a top cover 186 coupled to the sidewall 182.

[0158] The rotary joint 300 may include a damping plate 302 attached to a top cover 186 via screws or other fasteners. The top cover 186 may be part of the rotary joint module. The damping plate 302 may be coupled to a motor mounting plate 304 via screws 306. The rotary joint 300 may have a central axis 308, about which a motor 310 may rotate. The motor 310 may be coupled to the motor mounting plate 306 via screws 312. A rotatable base 314 may be attached to the motor 310 via screws 316. Thus, the rotatable base may be rotatable relative to the motor mounting plate 304 via the motor. A bearing housing 316 may be coupled to the rotatable base 314 via a support 318. The rotatable base 314 may define a suspension tab 320, which may be coupled to a flexible circuit assembly 322.

[0159] In some optional aspects, the top cover 186 may include at least one input device (e.g., a button) configured to start and stop treatment (e.g., TTField or heat). Optionally, the at least one input device may include multiple input devices, each configured to control the operation of different components of the system. In some aspects, the respective input devices may be configured to control TTField application and heat application. In a further aspect, the input devices may be configured to start and stop the operation of the rotary joint. In a further aspect, the top cover 186 may include a display configured to display information such as, for example, experiment identification information (e.g., cage number, electrode type) or operating mode (e.g., idle, treatment, heating, pause). In various aspects, the top cover 186 may provide a communication port that can communicate with the rotary joint 300 to provide a signal generator 12 ( Figure 1 Communication between the treatment / control heater assembly and the treatment heater assembly.

[0160] Also refer to Figure 13 and Figure 15 The flexible circuit assembly 322 may include a printed circuit board (PCB) 324 to which an input / output connector 326 is attached. The connector 326 may provide communication to the upper portion of the rotary joint. The PCB 324 may define a patterned portion 328 extending between a base portion 330 and a cable connector end 332. The patterned portion 328 may have a structure that allows the PCB 324 to be twisted, such that the connector end 332 can pivot relative to the base portion 330, as further described herein. In an exemplary aspect, the patterned portion 328 may have a serpentine, wavy, zigzag, or wave-like pattern. A pair of sensor connector portions 334 may extend from the base portion 330.

[0161] Also refer to Figure 18A , Figure 18B and Figure 19 The bearing housing 316 may accommodate a bearing 340 (e.g., a ball bearing or a nylon bearing) that receives and supports a pivot 342 within its inner race. The pivot 342 may define a groove 344 therein that receives a connector end 332 of a printed circuit board 324. The groove 344 receives and engages the connector end 332 such that the pivot can pivot accordingly as the connector end pivots about a central axis 308. (Although the figures show the connector end 332 held in place as the pivot pivots, it should be understood that in use, the connector end pivots together with the pivot.) The pivot 342 may define a cantilevered tab 346 extending parallel to the central axis 308.

[0162] A centering spring 348 may extend from a support 350 attached to the bearing housing 316 to engage a protrusion 352 or other radially extending surfaces spaced apart from or extending away from the central axis 308 of the rotary joint 300. The centering spring 348 can bias the pivot 316 to a neutral position 354. In use, the pivot can be in the neutral position 354 when the printed circuit board 324 is not subjected to or substantially not subjected to torque.

[0163] refer to Figure 15 , Figure 18A , Figure 18B and Figure 19 A pair of sensors 356 (e.g., electro-optic sensors, such as the VISHAY TCPT1600X01 sensor) can be attached to the bearing housing 316 via their respective sensor mounts 358. The sensors 356 can communicate with the PCB 324 at the sensor connector portion 334. The sensors 356 can also communicate with a processor (e.g., a PLC controller on circuit board 359, such as...) Figure 23 (as shown in the diagram) communication. The sensor may have a light source, a photodetector, and an optical path between the light source and the photodetector. Sensor 356 may be positioned such that when pivot 316 is in the neutral position, cantilever tab 346 can block both sensors 356. When the test object moves within the cage, it can allow cable 204 ( Figure 8 The pivot body is twisted, thereby applying torsion to the pivot body 342 and causing the pivot body to pivot from its neutral position. When the pivot body moves from the neutral position 350 along the first direction 360 (see...) Figure 19When fully pivoted, the cantilever tab 346 can be positioned outside the optical path of the first sensor 356A, which can detect light from a light source in the photodetector. In this way, the rotary joint 300 can detect cable twisting along a first direction. The processor can rotate the motor 310 along the first direction 360 to alleviate the twisting in the cable. Optionally, the rotary joint can be configured to remain stationary until a minimum threshold angle is reached with respect to the neutral position 350 to prevent excessive movement that could cause motor wear. Optionally, the motor can rotate a minimum angular distance to minimize an excessive number of minute movements. Similarly, when the pivot is fully pivoted from the neutral position 350 along a second direction 362 (opposite to the first direction), the second optical sensor 356B can detect this, and the processor can rotate the motor along the second direction to alleviate the twisting in the cable. In this way, the rotary joint can limit the amount of twisting in the cable 204, thereby allowing the test object to move freely within the cage.

[0164] Limiter 364 may be attached to each sensor mount 358. Each limiter 364 may include a central extension that can be used as a stop to prevent the pivot from pivoting from the neutral position 350 beyond a threshold angle, thereby preventing the printed circuit board 324 from breaking.

[0165] Pivot 342 may define connector 380, which is configured to receive connector ends 206, 206', 406, 406' of a flexible circuit board and electrically connect the flexible circuit board to PCB 332. For example, connector 380 may include a USB-C connector (e.g., a female USB-C connector) complementary to the connector ends of the flexible circuit board. Optionally, connector 380 may include a CAN bus connector.

[0166] The rotary joint may include a slip ring that can maintain electrical communication, thereby enabling communication between the treatment assembly 200 (or treatment assembly 200' or treatment assembly 200"), the heater assembly 400 (or heater assembly 400'), and the TTField generator 18. The slip ring can enable communication through at least twenty communication channels.

[0167] The computing device 1001 can communicate with the rotary joint 300 (e.g., via cable 20 or another cable) to track and / or record various measurements. In a further aspect, the computing device 1001 can be embodied in a PCB 359. Figure 23The controller on the rotary joint. For example, the metric may include some or all of the following: number of rotations, number of rotations within a first selected duration, frequency of motor movement, frequency of motor movement within a second selected duration, number of motor movements including changes in direction, number of motor movements including changes in direction within a third selected duration, duration of constant movement, and a log of motor movement and the corresponding time of motor movement. Such a metric can indicate abnormal behavior in the test subject. Further, such a metric can indicate a malfunction of the rotary joint 300. In some aspects, a warning (e.g., a warning light or audible alarm) can be activated to notify the user that the rotary joint 300 has malfunctioned or that the test subject is behaving abnormally. In a further aspect, the computing device or controller communicating with the rotary joint can be configured to receive user input. The user input can define one or more thresholds, such as, for example, a frequency threshold (e.g., the frequency of change along the direction of rotation) or a time threshold (e.g., the duration of a constant rotation threshold). A warning can be activated when the respective threshold is exceeded.

[0168] In a further aspect, the computing device can generate and output a log report that includes at least one of the collected metrics. The log report can further output a metric comparison based on a comparison with an average metric. The average metric can be an average metric for similar test subjects, or an average metric for a given test subject over a selected time period (e.g., over an hour, a day, or a week). For example, the average metric could include the average amount of movement the test subject makes each day. Therefore, it is envisioned that a decrease in daily movement could indicate a deterioration in the health of the test subject. It is further envisioned that excessively high or low movement compared to other similar test subjects could indicate the relative health of the test subject. It is further envisioned that monitoring various metrics could allow for the identification of modifications to ensure the survival of animal test subjects until the end of the experimental period.

[0169] refer to Figure 1Multiple test subjects 12 in experimental group 14 may be equipped with their own treatment components. Treatment components may be selected to treat specific tumors (e.g., treatment component 200 for organ tumors or treatment component 200' for subcutaneous tumors). The treatment components may be appropriately sized. For example, for organ tumors, the longitudinal length of the treatment component may be adapted to wrap snugly around the torso circumference of the test subject at a given tumor location. For subcutaneous tumors, the through-holes and caps of the treatment component may be selected to fit the subcutaneous tumor with minimal excess space. As disclosed herein, the cable length of the treatment component may be selected to allow the test subject to move freely across the floor area of ​​the enclosure without providing excess length that could become tangled. Release pads may be removed to allow adhesive to adhere to the test subject's skin. Hair on the test subject may be removed at the application area using a trimming device and / or depilatory cream (e.g., VEET depilatory cream). Similarly, test subjects 12 in the control group may be equipped with their own control heater treatment component 400 or control heater treatment component 400' (to match the counterparts in the experimental group).

[0170] The treatment component can be positioned on the body such that the electrodes are as close as possible to the tumor localization. In various aspects, the treatment component (or control heater component) can be positioned on the test subject's body to minimize or eliminate disruption to natural movement. For example, the treatment component can be positioned away from the hind or forelegs of the mouse to allow for natural movement. The treatment component can be selected based at least in part on the size of the mouse. For example, a narrow treatment component can be positioned on mice weighing less than 23 grams, and a wide treatment component can be positioned on mice weighing more than 23 grams.

[0171] The treatment array (or control heater assembly) can be oriented such that the cables extend toward the tail / rear end of the test subject. It is envisioned that the subcutaneous treatment assembly and control heater assembly can have formed bends (e.g., 90-degree bends) that allow the cables to extend to the middle of the test subject's back and then extend along the back toward the rear end. The formed bends can be provided in either direction, depending on the side of the test subject on which the subcutaneous tumor is located. An adhesive (e.g., a plaster) can be applied to the test subject to facilitate adhesion of the treatment assembly (or control heater assembly).

[0172] Test subjects can be placed within their respective enclosures of the cage assembly. The connector end of each treatment assembly and control heater treatment assembly 400 can be attached to its respective rotary joint.

[0173] The treatment component can be controlled to provide TTFields to the test subjects. For example, refer to... Figure 20 and Figure 21TTFields from 50-500 kHz (optionally 150-500 kHz) can be delivered to organ tumors 22 ( Figure 20 ) or subcutaneous tumor 22' ( Figure 21 Optionally, TTFields can be delivered sequentially along separate propagation axes (to provide alternating fields). For example, a first pair of opposing electrodes can apply a first electric field across the tumor along a first propagation axis 60. A second pair of electrodes, positioned with a second propagation axis 62 perpendicular or substantially perpendicular to the first propagation axis, can alternate with the first pair of opposing electrodes to provide alternating fields across the tumor. For the treatment assembly 200, each electrode can cooperate with its respective electrode positioned furthest from its location (e.g., on the opposite side of the subject's body) to provide alternating TTFields. Optionally, each electrode can be independently controlled to provide a customized treatment. The control heater treatment assembly can be controlled via a TTField generator or other controller to provide null control heat to match or substantially match the temperature of the treatment assembly. Treatment can optionally last for approximately 1-2 weeks.

[0174] Tumors in the experimental and control groups can be compared during and after treatment. For example, for subcutaneous tumors, the cap can be removed to expose the tumor while the treatment component is attached to the test subject, and the tumor size can be measured with calipers. Organ tumors can be measured via scans such as magnetic resonance imaging (MRI), ultrasound (US), or computed tomography (CT). The treatment component can be removed before such scans.

[0175] Computing device

[0176] Figure 22 A system 1000 is shown, which includes an exemplary configuration of a computing device 1001 for use in system 10.

[0177] The computing device 1001 may include one or more processors 1003, system memory 1012, and a bus 1013 that connects various components of the computing device 1001 (including the one or more processors 1003) to the system memory 1012. In the case of multiple processors 1003, the computing device 1001 can utilize parallel computing.

[0178] Bus 1013 may include one or more of several possible bus structures, such as memory bus, memory controller, peripheral bus, accelerated graphics port, and processor or local bus using any of the various bus architectures.

[0179] The computing device 1001 is operable on and / or includes various computer-readable media (e.g., non-transitory). The computer-readable media can be any available medium accessible by the computing device 1001, and includes non-transitory, volatile and / or non-volatile media, removable and non-removable media. The system memory 1012 has a computer-readable medium in the form of volatile memory (such as random access memory (RAM)) and / or non-volatile memory (such as read-only memory (ROM)). The system memory 1012 can store data, such as temperature data 1007 (i.e., data from signals received from electrodes); and / or program modules, such as operating system 1005 and TTField-provided software 1006, which the one or more processors 1003 can access and / or operate on.

[0180] The computing device 1001 may also include other removable / non-removable, volatile / non-volatile computer storage media. The mass storage device 1004 can provide non-volatile storage for computer code, computer-readable instructions, data structures, program modules, and other data for the computing device 1001. The mass storage device 1004 may be a hard disk, removable disk, removable optical disk, magnetic tape cassette or other magnetic storage device, flash memory card, CD-ROM, digital versatile disc (DVD) or other optical storage device, random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), etc.

[0181] Any number of program modules can be stored on the mass storage device 1004. Operating system 1005 and TTField providing software 1006 can be stored on the mass storage device 1004. One or more of operating system 1005 and TTField providing software 1006 (or some combination thereof) may include program modules and TTField providing software 1006. Temperature data 1007 can also be stored on the mass storage device 1004. Temperature data 1007 can be stored in any of one or more databases known in the art. The databases can be centralized or distributed across multiple locations within network 1015.

[0182] Users can use input devices (not shown) to input commands and information into computing device 1001. Such input devices include, but are not limited to, keyboards, clicking devices (e.g., computer mice, remote controls), microphones, joysticks, scanners, tactile input devices (such as gloves and other body coverings), motion sensors, etc. These and other input devices can be connected to the one or more processors 1003 via human-machine interface 1002 coupled to bus 1013, but can also be connected via other interfaces and bus structures (such as parallel ports, game ports, IEEE 1394 ports (also known as FireWire ports), serial ports, network adapter 1008, and / or Universal Serial Bus (USB)).

[0183] Display device 1011 may also be connected to bus 1013 using an interface such as display adapter 1009. It is envisioned that computing device 1001 may have more than one display adapter 1009 and more than one display device 1011. Display device 1011 may be a monitor, LCD (liquid crystal display), light-emitting diode (LED) display, television, smart lens, smart glass, and / or projector. In addition to display device 1011, other output peripherals may also include components that can be connected to computing device 1001 using input / output interface 1010, such as speakers (not shown) and printers (not shown). Any step and / or result of these methods may be output (or caused to be output) to an output device in any form. Such output may be any form of visual representation, including but not limited to text, graphics, animation, audio, haptic feedback, etc. Display 1011 and computing device 1001 may be part of a single device or separate devices.

[0184] Computing device 1001 can operate in a network environment via a logical connection to one or more remote computing devices 1014a, 1014b, 1014c. The remote computing devices 1014a, 1014b, 1014c can be personal computers, computing stations (e.g., workstations), portable computers (e.g., laptops, mobile phones, tablets), smart devices (e.g., smartphones, smartwatches, activity trackers, smart clothing, smart accessories), security and / or monitoring devices, servers, routers, network computers, peer-to-peer devices, edge devices, or other common network nodes. A network 1015 (such as a local area network (LAN) and / or a general wide area network (WAN)) can be used to establish the logical connection between computing device 1001 and the remote computing devices 1014a, 1014b, 1014c. This network connection can be achieved through a network adapter 1008. The network adapter 1008 can be implemented in both wired and wireless environments. This networking environment is common and routine in residential, office, and enterprise-wide computer networks, intranets, and the Internet. It is envisioned that the remote computing devices 1014a, 1014b, 1014c may optionally have some or all of the components disclosed as part of the computing device 1001.

[0185] Application programs and other executable program components (such as operating system 1005) are shown herein as discrete blocks; however, it should be recognized that such programs and components may reside in different storage units of computing device 1001 at various times and be executed by one or more processors 1003 of computing device 1001. Implementations of electrode data processing software 1006 may be stored on or transmitted across some form of computer-readable medium. Any disclosed methods may be executed by processor-executable instructions embodied on a computer-readable medium.

[0186] Exemplary aspects

[0187] In view of the products, systems, and methods described and their variations, certain more specific aspects of the invention are described below. However, these specifically described aspects should not be construed as limiting any of the different claims that incorporate the different or more general teachings described herein, or as limiting the “specific” aspects in some respects beyond the inherent meaning of the language used in the writing herein.

[0188] Aspect 1A: A cage assembly comprising: at least one enclosure, each enclosure having: a floor defining a floor area having a principal dimension; a cover having a bottom surface, wherein a distance between the bottom surface of the cover and the floor defines a cage height; and at least one sidewall extending between the floor and the cover, wherein the ratio of the cage height of each enclosure to the principal dimension of the floor area is at least 0.70.

[0189] Aspect 2A: The cage assembly according to aspect 1A, wherein the at least one enclosure includes a first enclosure and a second enclosure.

[0190] Aspect 3A: The cage assembly according to aspect 1A, wherein the at least one enclosure is composed of a first enclosure and a second enclosure.

[0191] Aspect 4A: The cage assembly according to aspect 2A or aspect 3A, wherein the first enclosure and the second enclosure share a common sidewall that separates the floor area of ​​the first enclosure from the floor area of ​​the second enclosure.

[0192] Aspect 5A: The cage assembly according to aspect 4A, wherein the common sidewall defines at least one opening between the first enclosure and the second enclosure.

[0193] Aspect 6A: The cage assembly according to aspect 5A, wherein each of the first enclosure and the second enclosure includes a respective shielding sub-assembly extending inwardly from the common sidewall within the enclosure, and wherein the shielding sub-assembly at least partially surrounds the opening within the common sidewall.

[0194] Aspect 7A: The cage assembly according to any one of the preceding aspects, wherein the floor of each enclosure defines a corner, and each corner of the floor of the enclosure has a radius of at least 17 mm.

[0195] Aspect 8A: The cage assembly according to any one of the preceding aspects, wherein the principal dimension of the floor area of ​​each enclosure is not greater than 250 mm.

[0196] Aspect 9A: The cage assembly according to any one of Aspects 2A-8A, wherein the floor of the first enclosure and the second enclosure is integrally constructed.

[0197] Aspect 10A: A cage assembly according to any one of Aspects 2A-9A, wherein the covers of the first enclosure and the second enclosure are integrally constructed as a cover assembly.

[0198] Aspect 11A: The cage assembly according to aspect 10A, wherein the cover assembly includes a first opening and a second opening respectively provided in communication with the first enclosure and the second enclosure, wherein the first opening is configured to provide communication with a first cable, and wherein the second opening is configured to provide communication with a second cable.

[0199] Aspect 12A: The cage assembly according to aspect 11A, wherein the cover assembly includes a first rotary joint assembly and a second rotary joint assembly positioned in a covering relationship with the first opening and the second opening, respectively, wherein the first rotary joint assembly is configured to receive a proximal portion of the first cable, and wherein the second rotary joint assembly is configured to receive a proximal portion of the second cable.

[0200] Aspect 13A: The cage assembly according to aspect 12A, wherein each of the first rotary joint assembly and the second rotary joint assembly includes a motor housing and a motor received within the motor housing, wherein the motor of the first rotary joint assembly is configured to be coupled to a first cable to allow adjustment of the first cable, and wherein the motor of the second rotary joint assembly is configured to be coupled to a second cable to allow adjustment of the second cable.

[0201] Aspect 14A: The cage assembly according to aspect 1A, wherein the at least one enclosure includes a first enclosure, wherein the cover includes a rotary joint assembly positioned in a covering relationship with the first enclosure, wherein the cover further includes an opening providing communication with the first enclosure, wherein the opening is configured to provide communication between the cable and the rotary joint assembly.

[0202] Aspect 15A: The cage assembly according to aspect 14A, wherein the rotary joint assembly includes a motor, wherein the motor is configured to be coupled to a proximal portion of the cable to allow adjustment of the movement of the cable.

[0203] Aspect 16A: The cage assembly according to any one of aspects 4A-13A, wherein the at least one sidewall of each of the first enclosure and the second enclosure further comprises: a front sidewall; a rear sidewall; and a transverse sidewall that is opposite to the common sidewall and extends between the front sidewall and the rear sidewall.

[0204] Aspect 17A: The cage assembly according to aspect 16A, wherein at least a portion of the front sidewalls of the first enclosure and the second enclosure are integrally constructed.

[0205] Aspect 18A: The cage assembly according to aspect 16A or aspect 17A, wherein the rear sidewalls of the first enclosure and the second enclosure are integrally constructed.

[0206] Aspect 19A: The cage assembly according to aspect 17A, wherein the front sidewalls of the first enclosure and the second enclosure include: a base portion fixed to the lateral sidewalls of the first enclosure and the second enclosure; a door pivotally coupled to the base portion, wherein the door is configured to move about and between: a closed position, wherein the door cooperates with the front sidewalls, lateral sidewalls, and rear sidewalls of the first enclosure and the second enclosure, and the cover, to enclose an interior space within the cage assembly; and an open position, wherein the interior space of the cage assembly is accessible.

[0207] Aspect 20A: The cage assembly according to aspect 19A, wherein the door is pivotally connected to the base portion via a hinge connector.

[0208] Aspect 21A: The cage assembly according to aspect 19A or aspect 20A further includes a latch mechanically coupled to the door, wherein the latch is movable about and between: a latch position that prevents pivoting movement of the door when the door is in the closed position; and an unlock position that allows pivoting movement of the door relative to the base portion.

[0209] Aspect 22A: The cage assembly according to any one of the preceding aspects, wherein the floor, the cover and the at least one sidewall of each enclosure comprises polycarbonate.

[0210] Aspect 23A: The cage assembly according to any one of the preceding aspects, wherein at least a portion of the cover of each enclosure and at least one sidewall is transparent.

[0211] Aspect 24A: The cage assembly according to any one of the preceding aspects, wherein the cover defines an opening configured to receive an electrical wire.

[0212] Aspect 25A: The cage assembly according to any one of the preceding aspects, wherein the floor of each enclosure comprises bedding material.

[0213] Aspect 26A: The cage assembly according to any one of the preceding aspects, wherein at least one sidewall of each enclosure includes a ventilation opening.

[0214] Aspect 27A: The cage assembly according to aspect 26A further includes at least one filter, said at least one filter being configured to cover at least one ventilation opening of said at least one sidewall.

[0215] Aspect 28A: The cage assembly according to aspect 27A further includes a frame configured to mechanically connect the filter to the at least one sidewall.

[0216] Aspect 29A: The cage assembly according to aspect 27A, wherein the cage assembly is sealed such that all or substantially all of the ventilation to each enclosure travels through the at least one filter before entering the ventilation opening.

[0217] Aspect 30A: The cage assembly according to any one of the preceding aspects, wherein the sidewalls of each enclosure have equal lengths.

[0218] Aspect 31A: The cage assembly according to any one of the preceding aspects, wherein the ratio of the cage height of each of the at least one enclosure to the main dimension of the floor area is at least 1.0.

[0219] Aspect 32A: A cage assembly comprising: at least one enclosure, each enclosure having: a floor defining a floor area having a principal dimension; a cover having a bottom surface, wherein the distance between the bottom surface of the cover and the floor defines a cage height; and at least one sidewall extending between the floor and the cover, wherein the height h is a function of the principal dimension Y of the floor according to the following formula: h ≥ (Y² – 6400) / 320, where h and Y are in millimeters.

[0220] Aspect 33A: A method comprising: placing an animal test subject within each enclosure of a cage assembly according to any one of the preceding aspects; and connecting a distal end of a cable to the animal test subject within each enclosure, wherein at least 90% of the floor area of ​​the enclosure is accessible to the animal test subject.

[0221] Aspect 34A: The method of claim 33, further comprising: connecting the proximal end of each cable to a rotary joint assembly, wherein each cable has an operating portion having an operating length, wherein each operating length is selected such that each test subject is not in a position within its respective enclosure, i.e., in which the spacing between the operating portions of the cables is within a threshold distance of the floor.

[0222] Aspect 35A: The method according to aspect 33A or aspect 34A, wherein the animal test subject is a mouse.

[0223] Aspect 36A: The method according to aspect 35A, wherein the cage assembly includes a first enclosure and a second enclosure, wherein a first mouse is placed in the first enclosure, and wherein a second mouse is placed in the second enclosure.

[0224] Aspect 37A: The method according to aspect 36A, wherein the first enclosure and the second enclosure share a common sidewall that separates the floor area of ​​the first enclosure from the floor area of ​​the second enclosure, wherein the common sidewall defines at least one opening between the first enclosure and the second enclosure, and wherein the at least one opening allows communication between the first mouse and the second mouse.

[0225] Aspect 38A: The method according to any one of aspects 33A-37A, wherein the wire is connected to an animal test subject via a treatment assembly including a transducer array.

[0226] Aspect 39A: The method according to aspect 38A further includes: using the wire and the treatment component to apply an electric field to an animal test subject within at least one enclosure.

[0227] Aspect 40A: The method according to aspect 39A, wherein the animal test subject has a tumor, and wherein the electric field is a tumor therapeutic field.

[0228] Aspect 41A: The method according to any one of aspects 33A-40A further comprises: inspecting or approaching the animal test subject through the cage assembly without removing the animal test subject from the cage assembly.

[0229] Aspect 42A: The method according to any one of aspects 33A-41A further comprises: removing the animal test subject from the cage assembly; and autoclaving the floor, the cover and the at least one sidewall of each enclosure.

[0230] Aspect 1B: A therapeutic assembly comprising: an inner layer having an inner surface and an outer surface, wherein the inner layer defines a plurality of openings extending therethrough; a plurality of plates, each plate being at least partially received within a respective opening of the plurality of openings in the inner layer; a therapeutic circuit system comprising: a cable including a plurality of electrical leads; and a plurality of electrical lead ends, each electrical lead being electrically connected to a respective electrical lead end of the plurality of electrical lead ends; and a cover layer attached to the outer surface of the inner layer and covering the plurality of electrical lead ends of the cable, wherein the plurality of lead ends contact a respective plate of the plurality of plates to define a plurality of electrodes, each of the plurality of electrodes including a respective lead end and a respective plate.

[0231] Aspect 2B: The therapeutic assembly according to aspect 1B, wherein at least one of the plurality of plates comprises a ceramic plate.

[0232] Aspect 3B: The therapeutic assembly according to aspect 1B, wherein at least one of the plurality of plates comprises a glass plate.

[0233] Aspect 4B: A treatment assembly according to any one of aspects 1B-3B, wherein at least one of the plurality of electrodes is configured to generate an electric field through a corresponding plate of the plurality of plates.

[0234] Aspect 5B: A treatment assembly according to any one of Aspects 1B-4B, wherein the plurality of electrodes of the treatment circuit system have respective top surfaces, and wherein the cover layer extends across the top surfaces of the electrodes of the cable.

[0235] Aspect 6B: A treatment assembly according to any one of aspects 1B-5B, wherein each of the plurality of plates has a lower surface and an opposing upper surface, wherein the treatment assembly further includes a hydrogel layer on the lower surface of each of the plurality of plates.

[0236] Aspect 7B: The treatment component according to any one of aspects 1B-6B, wherein the treatment circuit system further includes at least one temperature sensor.

[0237] Aspect 8B: A treatment component according to any one of Aspects 1B-7B, wherein the inner layer and the overlying layer cooperate to define a pore through the treatment component, wherein the pore is configured to receive a subcutaneous tumor.

[0238] Aspect 9B: The treatment component according to aspect 8B further includes a cap that extends across the orifice and defines therein a receptor configured to receive the subcutaneous tumor, wherein the cap is attached to the covering layer.

[0239] Aspect 10B: A treatment assembly according to aspect 8B or aspect 9B, wherein the plurality of plates are positioned radially outside the aperture defined through the treatment assembly.

[0240] Aspect 11B: The treatment assembly according to aspect 9B or aspect 10B, wherein the cap includes a peripheral edge, and wherein the treatment assembly further includes an adhesive ring that covers the peripheral edge and secures the cap to the cover layer.

[0241] Aspect 12B: A treatment component according to any one of Aspects 1B-11B, wherein the treatment component has a longitudinal dimension in a pre-use configuration, wherein the covering layer comprises a biocompatible nonwoven adhesive, wherein the nonwoven adhesive is elastic along the longitudinal dimension.

[0242] Aspect 13B: The treatment assembly according to aspect 12B, wherein, in the configuration of use, the cable extends perpendicularly or substantially perpendicularly to the longitudinal dimension.

[0243] Aspect 14B: A therapeutic component according to any one of Aspects 1B-13B, wherein the inner layer comprises a biocompatible, breathable polyurethane adhesive on the inner surface of the inner layer.

[0244] Aspect 15B: The therapeutic component according to aspect 14B, wherein the covering layer has an inner surface comprising a biocompatible nonwoven adhesive.

[0245] Aspect 16B: A treatment assembly according to any one of aspects 1B-15B, wherein the plurality of electrodes comprises a plurality of electric field generating electrodes, wherein the plurality of electric field generating electrodes are configured to transmit an electric field through a corresponding plate of the plurality of plates.

[0246] Aspect 17B: The treatment assembly according to aspect 16B, wherein the treatment circuit system further includes a plurality of thermistors.

[0247] Aspect 18B: The therapeutic assembly according to aspect 17B, wherein each of the plurality of electrodes and each of the plurality of thermistors communicates with each of the plurality of plates.

[0248] Aspect 19B: A treatment component according to any one of aspects 1B-18B, wherein the weight of the treatment component is less than 2.5 grams.

[0249] Aspect 20B: A treatment component according to any one of aspects 1B-19B, wherein the treatment component is sufficiently flexible to circumferentially conform to a portion of the torso of the animal test subject.

[0250] Aspect 21B: A treatment assembly according to any one of aspects 1B-20B, wherein the cable includes an end connector positioned at one end of the cable opposite to the plurality of electrodes, wherein the end connector is configured to allow connection of the cable to an electrical signal generator.

[0251] Aspect 22B: The therapeutic component according to aspect 14B further includes a release layer that contacts a biocompatible, breathable polyurethane adhesive on the inner surface of the inner layer.

[0252] Aspect 23B: The therapeutic component according to aspect 22B, wherein the release layer has a shape complementary to the shape of the covering layer.

[0253] Aspect 24B: A treatment component according to any one of aspects 1B-23B, wherein the covering layer defines at least one tab portion extending beyond the inner layer.

[0254] Aspect 25B: The therapeutic assembly according to aspect 2B, wherein the at least one tab portion comprises two opposing tab portions that are complementary to each other when the cover layer defines a circumferential ring.

[0255] Aspect 26B: A treatment component according to any one of aspects 1B-7B or aspects 12B-25B, wherein the plurality of openings comprises a plurality of longitudinally spaced openings.

[0256] Aspect 27B: The treatment assembly according to any one of aspects 1B-26B, wherein the treatment circuit system and the cables are integrally constructed as a flexible printed circuit board.

[0257] Aspect 28B: A method of manufacturing a treatment assembly according to any one of aspects 1B-27B, the method comprising: positioning the plurality of plates within respective openings in the inner layer of the treatment assembly; positioning each of the plurality of electrodes of the treatment circuit system in contact with one of the plurality of plates; and attaching the cover layer to an outer surface of the inner layer, wherein the cover layer covers the plurality of electrodes of the treatment circuit system.

[0258] Aspect 29B: The method according to aspect 28B further includes applying a hydrogel layer to the lower surface of each of the plurality of plates.

[0259] Aspect 30B: The method according to aspect 29B, wherein the lower surfaces of at least two of the plurality of plates share a hydrogel layer.

[0260] Aspect 31B: A method comprising: electrically connecting at least a portion of the electrodes of a treatment assembly according to any one of aspects 1B-27B to an electrical signal generator; attaching the treatment assembly to an animal subject suffering from a tumor, wherein the plates of the treatment assembly surround at least a portion of the tumor; and using the electrical signal generator to generate an electrical signal; and using the at least a portion of the electrodes of the treatment assembly to generate an electric field from the electrical signal passing through a corresponding plate of the plurality of plates.

[0261] Aspect 32B: The method according to aspect 31B, wherein the tumor is an organ tumor, wherein, in the pre-use configuration, the plurality of openings and the plurality of plates are longitudinally spaced along the longitudinal axis of the treatment assembly, and wherein, in the use configuration, the plurality of openings and the plurality of plates are circumferentially spaced around the trunk of the animal test subject to surround the organ tumor.

[0262] Aspect 33B: The method according to aspect 31B, wherein the tumor is a subcutaneous tumor, wherein the plurality of openings are radially spaced from a hole extending through the treatment component, and wherein the hole receives at least a portion of the subcutaneous tumor.

[0263] Aspect 34B: The method according to aspect 33B further includes: positioning the cap on the subcutaneous tumor and securing the cap to the covering layer of the treatment component.

[0264] Aspect 35B: The method according to any one of Aspects 31B-34B, wherein generating the electrical signal using the electrical signal generator comprises: sequentially generating a first electrical signal and a second electrical signal, and wherein generating the electric field from the electrical signal using at least a portion of the electrodes of the treatment component comprises: using the first electrode and the second electrode to generate a first electric field across the tumor from the first electrical signal; and using the third electrode and the fourth electrode to generate a second electric field across the tumor from the second electrical signal.

[0265] Aspect 36B: According to the method of aspect 35B, wherein the first electric field and the second electric field have their respective propagation axes, and wherein the propagation axis of the first electric field intersects the propagation axis of the second electric field.

[0266] Aspect 37B: The method according to any one of Aspects 28B-36B, wherein using the electrical signal generator to generate the electric field comprises generating the electric field at a frequency between 50 and 500 kHz.

[0267] Aspect 38B: The method according to any one of Aspects 31B-37B, wherein the animal test subject is a member of an experimental group, and wherein the method further comprises: electrically connecting at least a portion of the electrodes of the control heating device according to any one of Aspects 39B-42B to an electrical signal generator; attaching the control heating device to a second animal test subject suffering from a tumor, wherein the second animal test subject is a member of a control group, wherein the heater of the second treatment component surrounds at least a portion of the tumor; and using the electrical signal generator to generate heat through the heater of the control heating device, wherein the at least a portion of the electrodes of the second treatment component delivers heat through corresponding plates of the plurality of plates, and wherein the heat generated by the control heating device simulates the heat generated by the first treatment component during the delivery of the electric field.

[0268] Aspect 39B: A control heating device comprising: a circuit system including: a plurality of zones positioned in a spaced configuration that matches the configuration of the plurality of electrodes of a treatment assembly as described in any one of aspects 1B-27B; at least one heater positioned in each of the plurality of zones; at least one temperature sensor; and a cable communicating with the at least one heater and the at least one temperature sensor of the circuit system.

[0269] Aspect 40B: The contrast heating apparatus according to aspect 39B, wherein the at least one temperature sensor comprises a plurality of temperature sensors, wherein each of the plurality of temperature sensors is located in each of the plurality of zones.

[0270] Aspect 41B: The comparative heating apparatus according to aspect 39B or aspect 40B, wherein the circuit system and the cables are integrally constructed as a flexible printed circuit board.

[0271] Aspect 42B: The contrast heating apparatus according to any one of aspects 39B-41B further comprises: an inner layer having an upper surface and including a plurality of openings, wherein each of the regions is disposed within one of the plurality of openings; and a covering layer extending across the upper surface of the inner layer.

[0272] Aspect 43B: A treatment assembly according to any one of aspects 1B-27B, wherein the plurality of electrodes comprises: a plurality of head electrodes configured to be positioned on the head of the test subject; and a plurality of trunk electrodes configured to be positioned on the trunk of the test subject.

[0273] Aspect 44B: The treatment assembly according to aspect 43B, wherein the treatment circuit system includes a serpentine portion extending from the plurality of torso electrodes to the plurality of head electrodes.

[0274] Aspect 45B: The treatment assembly according to aspect 43B or aspect 44B, wherein the plurality of head electrodes comprises: a first set of electrodes positioned on a first side relative to a median plane; and a second plurality of electrodes positioned on a second side opposite to the first side relative to the median plane, wherein the plurality of trunk electrodes comprises at least a first trunk electrode positioned on the first side and at least a second trunk electrode positioned on the second side.

[0275] Aspect 1C: A rotary joint assembly having a longitudinal axis and comprising: an upper portion; a lower portion rotatably coupled to the upper portion, wherein the lower portion has a connector configured to securely engage a cable, wherein the lower portion is configured to maintain electrical communication with the upper portion when the lower portion rotates relative to the upper portion; a motor disposed between the upper portion and the lower portion and configured to selectively rotate the lower portion; a sensor configured to detect torsion in the cable; and a controller communicating with the sensor and the motor, wherein, upon receiving from the sensor a signal indicating a threshold torsion in the cable, the controller is configured to rotate the motor in a direction corresponding to the direction of torsion in the cable.

[0276] Aspect 2C: The rotary joint assembly according to aspect 1C, wherein the connector of the lower portion is an electrical connector, wherein the electrical connector is configured to securely engage electrical cables.

[0277] Aspect 3C: The rotary joint assembly according to aspect 2C, wherein the electrical connector is configured for electrical communication with an electrical signal generator, and wherein the electrical connector is further configured to allow electrical communication between the electrical signal generator and the electrical cable.

[0278] Aspect 4C: A rotary joint assembly according to any one of Aspects 1C-3C, wherein the controller is configured to rotate the motor in a direction corresponding to the twist direction of the cable until the controller receives from the sensor a signal indicating that the twist in the cable has decreased below a second threshold.

[0279] Aspect 5C: The rotary joint assembly according to any one of Aspects 1C-4C further includes a mounting assembly attached to the upper portion of the rotary joint relative to the longitudinal axis, wherein the lower portion of the rotary joint is rotatably coupled to the mounting assembly via the upper portion of the rotary joint.

[0280] Aspect 6C: The rotary joint assembly according to aspect 5C, wherein the mounting assembly is configured to be fixed to the top surface of the cage.

[0281] Aspect 7C: The rotary joint assembly according to aspect 5C or aspect 6C, wherein the mounting assembly defines an opening communicating with the cable outlet.

[0282] Aspect 8C: A rotary joint assembly according to any one of Aspects 1C-7C, wherein the lower portion includes a base plate located below and engaging the motor, and wherein the motor imparts rotational force to the lower portion through the base plate.

[0283] Aspect 9C: A rotary joint assembly according to any one of Aspects 1C-8C, wherein the upper portion includes a support plate covering the motor.

[0284] Aspect 10C: A rotary joint assembly according to any one of Aspects 1C-9C, wherein the upper portion further includes a damping plate covering the motor.

[0285] Aspect 11C: A rotary joint assembly according to any one of Aspects 1C-10C, wherein the controller is configured to detect abnormal rotation of the rotary joint assembly, wherein the abnormal rotation is detected by at least one of the following: (1) one or more signals from the sensor indicating a change in the direction of rotation at a frequency exceeding a frequency threshold; or (2) one or more signals from the sensor indicating continuous rotation in a single direction exceeding a time threshold.

[0286] Aspect 12C: The rotary joint assembly according to aspect 11C, wherein the controller is configured to receive user input to define at least one of the frequency threshold or the time threshold.

[0287] Aspect 13C: The rotary joint assembly according to aspect 11C or aspect 12C further includes a warning indicator, wherein the controller is configured to activate the warning indicator when abnormal rotation is detected.

[0288] Aspect 14C: The rotary joint assembly according to aspect 13C, wherein the warning indicator includes at least one of a visible indicator or an audible indicator.

[0289] Aspect 15C: A rotary joint assembly according to any one of Aspects 1C-14C, wherein the sensor is a torque sensor comprising: a pivot body pivotally coupled about a pivot axis to the lower portion of the rotary joint and configured to be coupled to the cable such that torsion of the cable applies torque to the pivot body, thereby causing pivoting of the pivot body relative to the lower portion of the rotary joint, wherein the pivot body includes a radially extending surface extending radially outward relative to the pivot axis; and a spring configured to deflect the pivot body relative to the lower portion of the rotary joint. The pivot is positioned in a neutral position; a first electro-optic sensor and a second electro-optic sensor are radially spaced from the pivot axis of the pivot and are in their respective angular positions relative to the pivot, wherein, when the pivot pivots from the neutral position along a first direction for a first threshold angular distance, the radially extending surface of the pivot is configured to realize a state change in the first electro-optic sensor, and wherein, when the pivot pivots from the neutral position along an opposite second direction for a second threshold angular distance, the radially extending surface of the pivot is configured to realize a state change in the second electro-optic sensor.

[0290] Aspect 16C: The rotary joint assembly according to aspect 15C further includes a printed circuit board having a first portion rotatably fixed to the lower portion of the rotary joint, a second portion pivotable with the pivot body, and at least one cutout such that the printed circuit board includes a patterned portion that enables the first portion to pivot relative to the second portion.

[0291] Aspect 17C: A rotary joint assembly according to aspect 15C or aspect 16C, wherein each of the first electro-optic sensor and the second electro-optic sensor includes a light source, a photodetector, and an optical path between the light source and the photodetector, wherein when the pivot is in the neutral position, the radially extending surface of the pivot is configured to block the respective optical path of each of the first electro-optic sensor and the second electro-optic sensor, wherein a state change in the first electro-optic sensor when the pivot pivots from the neutral position along the first direction by a first threshold angular distance includes: the first electro-optic sensor no longer detects that the radially extending surface blocks the optical path of the first electro-optic sensor.

[0292] Aspect 18C: The rotary joint assembly according to aspect 17C, wherein the radially extending surface of the pivot body includes a protrusion.

[0293] Aspect 19C: A rotary joint assembly according to any one of Aspects 1C-18C, wherein the motor is a brushless universal joint fixed motor.

[0294] Aspect 20C: The rotary joint assembly according to any one of Aspects 1C-19C further includes the cable, wherein the cable has a proximal portion fixed to the connector and an opposing distal portion, wherein the cable is configured to move in response to a force applied to the distal portion of the cable.

[0295] Aspect 21C: The rotary joint assembly according to any one of Aspects 1C-20C further includes a slip ring configured to provide electrical communication between the upper and lower portions when the lower portion rotates relative to the upper portion.

[0296] Aspect 22C: A rotary joint assembly according to any one of Aspects 1C-21C, wherein the cable is part of a flexible printed circuit board.

[0297] Aspect 23C: A method comprising: securing a proximal portion of a cable to a connector of a rotary joint assembly according to any one of aspects 1C-22C; and, in response to a torsion in the cable in a first direction, using the controller to rotate the motor in a direction corresponding to the first direction of the torsion of the cable.

[0298] Aspect 24C: The method according to aspect 23C, wherein the cable is an electrical cable, and wherein the cable has a distal portion electrically connected to the electrode array.

[0299] Aspect 25C: The method according to aspect 24C, wherein the electrode array is coupled to an animal test subject.

[0300] Aspect 26C: The method according to aspect 25C, wherein the animal test subject is placed in a cage, and wherein the rotary joint assembly is fixed to the cage.

[0301] Aspect 27C: The method according to any one of aspects 23C-26C further comprises: using the controller to determine abnormal rotation based on at least one of the following: (1) one or more signals from the sensor indicating a change frequency in the direction of rotation exceeding a frequency threshold; or (2) one or more signals from the sensor indicating continuous rotation in a single direction exceeding a time threshold.

[0302] Aspect 28C: A system comprising: a rotary joint assembly as described in any one of aspects 1C-22C; a computing device including a memory in communication with at least one processor, wherein the memory includes instructions that, when executed, cause the at least one processor to perform a method comprising: storing in the memory at least one metric selected from the group consisting of: number of rotations, number of rotations within a first selected duration, frequency of motor movement, frequency of motor movement within a second selected duration, number of motor movements including changes in direction, number of motor movements including changes in direction within a second selected duration, duration of constant movement, and a log of motor movement and corresponding times of motor movement.

[0303] Aspect 29C: The rotary joint assembly according to aspect 28C, wherein the memory includes instructions that, when executed, cause the at least one processor to perform the method, the method further comprising: generating a log report, wherein the log report includes the at least one metric.

[0304] Aspect 30C: The rotary joint assembly according to aspect 29C, wherein the log report further includes at least one comparison of the at least one metric and the average value of the at least one metric over a specified time period.

[0305] Aspect 1D: A system comprising: a cage assembly according to any one of aspects 1A-32A; and a rotary joint assembly according to any one of aspects 1C-22C.

[0306] Aspect 2D: The system according to aspect 1D, further comprising a treatment component according to any one of aspects 1B-27B.

[0307] Aspect 3D: The system according to aspect 1D further includes a control heating device according to any one of aspects 39B-42B.

[0308] Aspect 4D: A method of using the system according to any one of aspects 1D-3D.

[0309] Although the foregoing invention has been described in some detail by way of illustration and examples for the purpose of clear understanding, certain changes and modifications may be practiced within the scope of the appended claims.

Claims

1. A cage assembly comprising: The first enclosure and the second enclosure, each enclosure having: Floor, which defines a floor area with primary dimensions; A lid having a bottom surface, wherein the distance between the bottom surface of the lid and the floor defines the cage height; and At least one sidewall extends between the floor and the cover. Wherein, the ratio of the cage height of each of the at least one enclosure to the main dimension of the floor area is at least 0.

70. The first enclosure and the second enclosure share a common sidewall that separates the floor area of ​​the first enclosure from the floor area of ​​the second enclosure. The common sidewall defines at least one opening between the first enclosure and the second enclosure. Each of the first enclosure and the second enclosure includes a respective shielding sub-assembly extending inward from the common sidewall within the enclosure, and wherein the shielding sub-assembly at least partially surrounds the opening within the common sidewall.

2. The cage assembly according to claim 1, wherein, The floor of each enclosure defines a corner, and each corner of the floor of the enclosure has a radius of at least 17 mm.

3. The cage assembly according to claim 1, wherein, The principal dimension of the floor area of ​​each enclosure is no greater than 250 mm.

4. The cage assembly according to claim 1, wherein, The floors of the first enclosure and the second enclosure are constructed integrally.

5. The cage assembly according to claim 1, wherein, The covers of the first enclosure and the second enclosure are integrally constructed as a cover assembly.

6. The cage assembly according to claim 5, wherein, The cover assembly includes a first opening and a second opening respectively providing communication with the first enclosure and the second enclosure, wherein the first opening is configured to provide communication with a first cable, and wherein the second opening is configured to provide communication with a second cable.

7. The cage assembly according to claim 6, wherein, The cover assembly includes a first rotary connector assembly and a second rotary connector assembly positioned in a covering relationship with the first opening and the second opening, respectively, wherein the first rotary connector assembly is configured to receive a proximal portion of the first cable, and wherein the second rotary connector assembly is configured to receive a proximal portion of the second cable.

8. The cage assembly according to claim 7, wherein, Each of the first rotary joint assembly and the second rotary joint assembly includes a motor housing and a motor received within the motor housing, wherein the motor of the first rotary joint assembly is configured to be coupled to a first cable to allow adjustment of the first cable, and wherein the motor of the second rotary joint assembly is configured to be coupled to a second cable to allow adjustment of the second cable.

9. The cage assembly according to claim 1, wherein, The at least one enclosure includes a first enclosure, wherein the cover includes a rotary joint assembly positioned in a covering relationship with the first enclosure, wherein the cover further includes an opening providing communication with the first enclosure, wherein the opening is configured to provide communication between the cable and the rotary joint assembly.

10. The cage assembly according to claim 9, wherein, The rotary joint assembly includes a motor, wherein the motor is configured to be coupled to a proximal portion of the cable to allow adjustment of the cable's movement.

11. The cage assembly according to claim 1, wherein, The at least one sidewall of each of the first enclosure and the second enclosure further includes: Anterior sidewall; The posterior sidewall; and A transverse sidewall, which is opposite to the common sidewall and extends between the front sidewall and the rear sidewall.

12. The cage assembly according to claim 11, wherein, At least a portion of the front sidewalls of the first enclosure and the second enclosure are integrally constructed.

13. The cage assembly according to claim 11, wherein, The rear sidewalls of the first enclosure and the second enclosure are integrally constructed.

14. The cage assembly according to claim 12, wherein, The front sidewalls of the first enclosure and the second enclosure include: The base portion, which is fixed to the lateral sidewalls of the first enclosure portion and the second enclosure portion; and A door, which is pivotally connected to the base portion, wherein the door is configured to move about a closed position and an open position and between the two: in the closed position, the door cooperates with the front sidewalls, lateral sidewalls and rear sidewalls of the first enclosure and the second enclosure, as well as the cover, to enclose the interior space within the cage assembly, and in the open position, the interior space of the cage assembly is accessible.

15. The cage assembly according to claim 14, wherein, The door is pivotally connected to the base portion via a hinge connector.

16. The cage assembly of claim 14, further comprising a latch mechanically coupled to the door, wherein, The latch is movable about a latched position and an unlocked position and between the two: the latched position prevents pivoting of the door when the door is in the closed position, and the unlocked position allows pivoting of the door relative to the base portion.

17. The cage assembly according to claim 1, wherein, The floor, the cover, and the at least one sidewall of each enclosure comprise polycarbonate.

18. The cage assembly according to claim 1, wherein, At least a portion of the cover and at least one sidewall of each enclosure is transparent.

19. The cage assembly according to claim 1, wherein, The cover defines an opening configured to receive electrical wires.

20. The cage assembly according to claim 1, wherein, The floor of each enclosure includes a mattress.

21. The cage assembly according to claim 1, wherein, At least one sidewall of each enclosure includes a ventilation opening.

22. The cage assembly of claim 21, further comprising at least one filter configured to cover at least one ventilation opening of the at least one sidewall.

23. The cage assembly of claim 22, further comprising a frame configured to mechanically connect the filter to the at least one sidewall.

24. The cage assembly according to claim 22, wherein, The cage assembly is sealed such that all or substantially all of the ventilation to each enclosure travels through the at least one filter before entering the ventilation opening.

25. The cage assembly according to claim 1, wherein, The sidewalls of each enclosure have equal lengths.

26. The cage assembly according to claim 1, wherein, The ratio of the cage height of each of the at least one enclosure to the main dimension of the floor area is at least 1.

0.

27. The cage assembly according to claim 1, wherein, According to the following formula, the cage height h is a function of the principal dimension Y of the floor area: h ≥ (Y 2 – 6400) / 320, where h and Y are in millimeters.

28. A method of using a cage assembly, comprising: Animal test subjects are placed within each enclosure of the cage assembly according to any one of the preceding claims; as well as Wherein, at least 90% of the floor area of ​​the enclosure is accessible to the animal test subjects.

29. The method according to claim 28, wherein, The animal subjects used in the experiments were rats.

30. The method according to claim 29, wherein, The cage assembly includes a first enclosure and a second enclosure, wherein a first mouse is placed in the first enclosure and a second mouse is placed in the second enclosure.

31. The method according to claim 30, wherein, The first enclosure and the second enclosure share a common sidewall that separates the floor area of ​​the first enclosure from the floor area of ​​the second enclosure, wherein the common sidewall defines at least one opening between the first enclosure and the second enclosure, and wherein the at least one opening allows communication between the first mouse and the second mouse.

32. The method of claim 28, further comprising: The animal subject can be inspected or approached through the cage assembly without removing the animal subject from the cage assembly.

33. The method of claim 28, further comprising: Remove the animal test subject from the cage assembly; as well as The floor, the cover, and the at least one sidewall of each enclosure are autoclaved.

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