Multi-wavelength photothermal and photodynamic melanoma laser therapeutic instrument

Abstract

The application provides a multi-wavelength photothermal and photodynamic melanoma laser treatment instrument, which comprises a shell, at least one laser element and at least one optical fiber probe in the shell, wherein the at least one laser element emits one or more laser beams with a wavelength of 600-1350 nm. The laser treatment instrument uses melanin in melanoma as a photosensitizer for photothermal and photodynamic treatment, and does not need to inject an external photosensitizer, thereby solving the problem of toxicity caused by injection of the photosensitizer. Meanwhile, the melanoma itself can induce photothermal and photodynamic treatment, and has good targeting, thereby solving the problem of targeting tumors by the photosensitizer. In addition, the laser treatment instrument adopts multi-wavelength laser combination and invasive optical fiber irradiation, can solve the problem that surface melanin blocks most light energy and cannot irradiate deep melanoma, and can realize deep melanoma treatment.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to a multi-wavelength photothermal and photodynamic laser therapy device for melanoma. Background Technology

[0002] Currently, the main clinical treatments for melanoma include surgical resection, radiotherapy, and chemotherapy. In recent years, phototherapy has also been widely applied to melanoma treatment. Phototherapy methods include photothermal therapy and photodynamic therapy. Photothermal therapy utilizes materials with high photothermal conversion efficiency, injected into the body, and uses targeted recognition technology to concentrate near the tumor tissue. Under the irradiation of an external light source (usually near-infrared light), the light energy is converted into heat energy to kill cancer cells. Photodynamic therapy is a new method for treating tumors using photosensitizing drugs and laser activation. Irradiating the tumor site with a specific wavelength activates the photosensitizing drugs that selectively accumulate in the tumor tissue, triggering a photochemical reaction that destroys the tumor. In the new generation of photodynamic therapy, the photosensitizing drugs transfer energy to the surrounding oxygen, generating highly reactive singlet oxygen. Singlet oxygen can react with nearby biomolecules to produce cytotoxicity, thereby killing tumor cells.

[0003] For example, the patent with application number 201810475100.8: an exosome, a method for preparing exosomes and its application in the preparation of drugs for superficial skin tumors, uses targeted peptide-modified nanoparticles and loads them with the photosensitizer indocyanine green (ICG). After the nanoparticles are transdermally administered, near-infrared light is used to trigger the ICG to generate heat and release singlet oxygen.

[0004] For example, patent application number 201910054759.0: a targeted phase change nanoparticle loaded with PD1 antibody and its preparation method and application. The targeted phase change nanoparticle loaded with PD1 antibody can be used in the preparation of anti-melanoma drugs to target and deliver PD1 antibody to melanoma tissue, increase the concentration of PD1 antibody in melanoma tissue, and at the same time, combined with Fe3O4 nanoparticle photothermal therapy to enhance the anti-tumor immune response of T cells in melanoma tissue, effectively improving the therapeutic efficacy of PD1 antibody.

[0005] However, existing phototherapy methods for melanoma all involve the external injection of photosensitizers, but photosensitizers often have problems with biotoxicity and poor tumor targeting. Summary of the Invention

[0006] The main objective of this invention is to provide a multi-wavelength photothermal and photodynamic laser therapy device for melanoma, aiming to solve the problem that existing melanoma phototherapy methods require external injection of photosensitizers.

[0007] To achieve the above objectives, the present invention proposes a multi-wavelength photothermal and photodynamic laser therapy device for melanoma, comprising:

[0008] case;

[0009] At least one laser element and at least one fiber optic probe are located within the housing;

[0010] The at least one laser element emits one or more laser beams, wherein the one or more laser beams have a wavelength of 600nm-1350nm.

[0011] Optionally, the laser element includes a first laser element and a second laser element, wherein the first laser element emits a laser wavelength of 750-1000nm and the second laser element emits a laser wavelength of 1000-1350nm.

[0012] Optionally, the laser therapy device further includes a third laser element, wherein the laser wavelength emitted by the third laser element is 600nm-750nm.

[0013] Optionally, the power density of the laser is 0.1-2 W / cm². 2 The irradiation time is 5-20 minutes.

[0014] Optionally, the power density of the fiber optic probe is 0.5-1 W / cm². 2 The irradiation time is 5-10 minutes.

[0015] This invention also proposes a treatment method for melanoma, comprising:

[0016] Irradiate the melanoma site with one or more laser beams with wavelengths of 600nm-1350nm;

[0017] Invasive fiber optic irradiation of the melanoma site was performed using at least one fiber optic probe.

[0018] Optionally, the laser is two beams, namely a first laser with a wavelength of 750-1000nm and a second laser with a wavelength of 1000-1350nm.

[0019] Optionally, the laser may further include a third laser with a wavelength of 600nm-750nm.

[0020] Optionally, the power density of the laser is 0.1-2 W / cm². 2 The irradiation time is 5-20 minutes.

[0021] Optionally, the power density of the fiber optic probe is 0.5-1 W / cm². 2 The irradiation time is 5-10 minutes.

[0022] The multi-wavelength photothermal and photodynamic laser therapy device for melanoma of this invention utilizes the melanin present in the melanoma itself as a photosensitizer for photothermal and photodynamic therapy, eliminating the need for external photosensitizer injection and solving the toxicity problem associated with photosensitizer injection. Simultaneously, the melanoma itself induces photothermal and photodynamic therapy, exhibiting good targeting, thus solving the problem of photosensitizer targeting the tumor. Furthermore, this laser therapy device employs a multi-wavelength laser combination, coupled with invasive fiber optic irradiation, which can address the issue of surface melanin trapping most of the light energy, preventing it from reaching deeper melanoma layers. This enables deep-level melanoma treatment, killing deep-seated melanoma tissue. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0024] Figure 1 This is an experimental result diagram comparing the photothermal heating of different tumor models in one embodiment of the present invention;

[0025] Figure 2 This is an experimental result diagram comparing the photothermal heating of different tumor models in another embodiment of the present invention;

[0026] Figure 3 This is a diagram showing the experimental results of photothermal and photodynamic therapy for melanoma according to the present invention.

[0027] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0029] This invention proposes a multi-wavelength photothermal and photodynamic laser therapy device for melanoma, which can directly treat melanoma with light without the need for external injection of photosensitizers.

[0030] In this embodiment of the invention, a multi-wavelength photothermal and photodynamic laser therapy device for melanoma is proposed, comprising: a housing; at least one laser element and at least one fiber optic probe located within the housing; wherein the at least one laser element emits one or more laser beams, the one or more laser beams having a wavelength of 600nm-1350nm.

[0031] Specifically, the housing constitutes the overall external structure of the laser therapy device, serving to protect the internal components. The laser element is selected from Er:YAG, pulsed CO2, Ho:YAG, Er:YAP, Er / Cr:YSGG, Ho:YSGG, Er:GGSG, Er:YLF, Tm:YAG, Ho / Nd:YAlO3, cobalt:MgF2, HF reagent, DF reagent, carbon monoxide, deep UV laser, diode laser, Nd:YAG laser with frequency increased to three times, and all combinations thereof.

[0032] Specifically, fiber optic probes are inserted into the melanoma site for invasive laser irradiation, allowing for phototherapy of deeper areas of the melanoma. Fiber optic probes possess excellent properties such as water resistance, high-temperature resistance, chemical corrosion resistance, and resistance to electromagnetic interference and nuclear radiation. The wavelength of the light emitted by the fiber optic probe can be the same as or different from the wavelength emitted by the laser element, but it should still be within the range of 600nm-1350nm.

[0033] The multi-wavelength photothermal and photodynamic laser therapy device for melanoma of this invention utilizes the melanin present in the melanoma itself as a photosensitizer for photothermal and photodynamic therapy, eliminating the need for external photosensitizer injection and solving the toxicity problem associated with photosensitizer injection. Simultaneously, the melanoma itself induces photothermal and photodynamic therapy, exhibiting good targeting, thus solving the problem of photosensitizer targeting the tumor. Furthermore, this laser therapy device employs a multi-wavelength laser combination, coupled with invasive fiber optic irradiation, which can address the issue of surface melanin trapping most of the light energy, preventing it from reaching deeper melanoma layers. This enables deep-level melanoma treatment, killing deep-seated melanoma tissue.

[0034] In one embodiment, the laser therapy device can perform photothermal therapy, which uses a combination of near-infrared biological window I (750-1000nm) laser and near-infrared biological window II (1000-1350nm) laser. Compared with near-infrared biological window I, near-infrared biological window II has a larger upper limit of light exposure energy that the skin can tolerate and a better laser penetration depth, which can achieve deep-level treatment of melanoma. At the same time, it is combined with an invasive fiber optic probe to penetrate into the melanoma and kill deep-level melanoma tissue.

[0035] Specifically, the laser therapy device can have one or two laser elements. Near-infrared biological window I laser and near-infrared biological window II laser can be emitted sequentially through the same laser element. Near-infrared biological window I laser can be emitted first, or near-infrared biological window II laser can be emitted first. Near-infrared biological window I laser and near-infrared biological window II laser can also be emitted sequentially or simultaneously through two different laser elements. No limitation is made here.

[0036] More specifically, the optical fiber probe can be illuminated simultaneously with the laser, or it can be illuminated sequentially with the laser. The order of illumination is not limited; the optical fiber probe can be illuminated before or after the laser.

[0037] In another embodiment, the laser therapy device can also perform photodynamic therapy, using a visible light wavelength (600-750nm) laser to irradiate the melanoma, combined with an invasive fiber optic probe, to kill the melanoma.

[0038] Specifically, the light irradiation of the fiber optic probe can be simultaneous with visible light wavelength laser irradiation, or they can be irradiated sequentially. The light irradiation of the fiber optic probe can be before or after the laser irradiation, and there is no limitation on this.

[0039] In a preferred embodiment, the laser therapy device can perform photothermal therapy and photodynamic therapy, using a combination of near-infrared biological window I (750-1000nm) laser, near-infrared biological window II (1000-1350nm) laser, and visible light wavelength (600-750nm) laser, and simultaneously using an invasive fiber optic probe to penetrate into the melanoma and kill deep-seated melanoma tissue.

[0040] Specifically, the laser therapy device described in this embodiment can have one, two, or three laser elements, which are not limited here. When there is one laser element, the near-infrared biological window I laser, the near-infrared biological window II laser, and the visible light wavelength laser are emitted sequentially through the same laser element, and the emission order is not limited. When there are two laser elements, for example, the near-infrared biological window I laser and the near-infrared biological window II laser can be emitted through the same laser element, while the visible light wavelength laser is emitted through another laser element. When there are three laser elements, the near-infrared biological window I laser, the near-infrared biological window II laser, and the visible light wavelength laser are emitted through different laser elements. The three lasers can irradiate simultaneously or sequentially, and the order is not limited.

[0041] Optionally, the laser element includes a first laser element and a second laser element, wherein the first laser element emits a laser wavelength of 750-1000nm and the second laser element emits a laser wavelength of 1000-1350nm.

[0042] Optionally, the laser therapy device further includes a third laser element, wherein the laser wavelength emitted by the third laser element is 600nm-750nm.

[0043] Optionally, the power density of the laser is 0.1-2 W / cm². 2 The irradiation time is 5-20 minutes.

[0044] Optionally, the power density of the fiber optic probe is 0.5-1 W / cm². 2 The irradiation time is 5-10 minutes.

[0045] This invention also proposes a treatment method for melanoma, comprising:

[0046] Irradiate the melanoma site with one or more laser beams with wavelengths of 600nm-1350nm;

[0047] Invasive fiber optic irradiation of the melanoma site was performed using at least one fiber optic probe.

[0048] It is understandable that laser irradiation of the melanoma site and invasive fiber optic irradiation of the melanoma site using a fiber optic probe can be performed simultaneously or sequentially. Laser irradiation can be performed before or after the melanoma site, and there is no limitation on this.

[0049] The melanoma treatment method of this invention utilizes the melanin present in the melanoma itself as a photosensitizer for photothermal and photodynamic therapy, eliminating the need for external photosensitizer injections and solving the toxicity problem associated with photosensitizer injections. Simultaneously, the melanoma itself induces photothermal and photodynamic therapy, exhibiting good targeting, thus solving the problem of photosensitizers targeting tumors. Furthermore, this method employs a combination of multi-wavelength lasers and invasive fiber optic irradiation, which can overcome the problem of surface melanin trapping most of the light energy and preventing it from reaching deeper melanoma layers, enabling deep-level melanoma treatment and killing deep-seated melanoma tissue.

[0050] In one embodiment, the treatment method uses a combination of near-infrared biological window I (750-1000nm) laser and near-infrared biological window II (1000-1350nm) laser. Compared with near-infrared biological window I, near-infrared biological window II has a larger upper limit of light exposure energy that the skin can tolerate and a better laser penetration depth, which can achieve deep treatment of melanoma. At the same time, it is combined with an invasive fiber optic probe to penetrate into the melanoma and kill deep melanoma tissue.

[0051] Specifically, near-infrared biological window I laser and near-infrared biological window II laser can irradiate the melanoma site sequentially or simultaneously, without limitation.

[0052] In another embodiment, the melanoma treatment method can also use visible light wavelength (600-750nm) laser to irradiate the melanoma site for photodynamic therapy, combined with an invasive fiber optic probe to kill the melanoma.

[0053] In a preferred embodiment, the treatment method uses a combination of near-infrared biological window I (750-1000nm) laser, near-infrared biological window II (1000-1350nm) laser, and visible light wavelength (600-750nm) laser to irradiate the melanoma site, while simultaneously using an invasive fiber optic probe to penetrate into the melanoma and kill deep-seated melanoma tissue.

[0054] Specifically, near-infrared biological window I laser, near-infrared biological window II laser, and visible wavelength laser can irradiate the melanoma site simultaneously, or they can be irradiated in a specific order. For example, near-infrared biological window I laser and near-infrared biological window II laser can be irradiated before or after the visible wavelength laser, without any limitation.

[0055] Optionally, the laser is two beams, namely a first laser with a wavelength of 750-1000nm and a second laser with a wavelength of 1000-1350nm.

[0056] Optionally, the laser may further include a third laser with a wavelength of 600nm-750nm.

[0057] Optionally, the power density of the laser is 0.1-2 W / cm². 2 The irradiation time is 5-20 minutes.

[0058] Optionally, the power density of the fiber optic probe is 0.5-1 W / cm². 2 The irradiation time is 5-10 minutes.

[0059] The embodiments of the present invention will be described in detail below with reference to specific examples. However, those skilled in the art will understand that the following examples are for illustrative purposes only and should not be considered as limiting the scope of the invention. Unless otherwise specified in the examples, conventional conditions or conditions recommended by the manufacturer are followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0060] Example 1: Comparison of photothermal heating in different tumor models

[0061] Three tumor models—B16 melanoma, A20 lymphoma, and SMMC-7721 liver tumor—were selected. Different tumors were irradiated with light, and the temperature rise of the three tumor models was observed after irradiation. The results are as follows: Figure 1 As shown in the figure. Among them, the B16 melanoma model showed significant temperature rise, at 0.5 W / cm². 2 Under irradiation with wavelengths of 808nm and 1064nm, the temperature can rise to 62 degrees Celsius after 10 minutes of irradiation. However, for A20 lymphoma and SMMC-7721 liver tumors that do not contain melanin, the maximum temperature can only rise to 30 degrees Celsius, which fully demonstrates the photothermal conversion performance of melanoma.

[0062] Example 2: Comparison of photothermal heating in different tumor models

[0063] We selected three tumor models: B16 melanoma, A20 lymphoma, and HCT116 tumor. We compared the temperature rise of different tumors under laser wavelengths of 808 nm and 1064 nm. The results are as follows: Figure 2 And as shown in Table 1. At 1 W / cm 2 After 10 minutes of laser irradiation, the temperature of B16 melanoma rapidly increased to 130 degrees, while that of A20 and HCT116 tumors only increased by 1-2 degrees. This further demonstrates the excellent photothermal conversion properties of melanoma itself.

[0064] Table 1. Temperature rise of different tumors under near-infrared light irradiation

[0065]

[0066] Example 3: Photothermal and Photodynamic Therapy for Melanoma

[0067] To highlight the therapeutic effects of photothermal and photodynamic therapy on melanoma, we selected three tumor models—B16 melanoma, A20 lymphoma, and SMMC-7721 liver tumor—for treatment comparison. Among them, A20 lymphoma and SMMC-7721 liver tumor are tumor models without melanin.

[0068] The basic approach involves using a combination of near-infrared lasers with good penetration (808nm and 1064nm) and visible light wavelengths (630nm or 660nm) to irradiate the lesions of three tumor models: B16 melanoma, A20 lymphoma, and SMMC-7721 liver tumor. For larger tumors where non-contact laser ablation is not feasible, a fiber optic probe is used to penetrate the tumor, with a power density of 1W / cm². 2 The irradiation time was 10 minutes, and the treatment was repeated every other day (day 0, day 2, day 4, ...) to eliminate the tumor. The results were as follows. Figure 3 As shown in the figure, by comparing the treatment effects on different tumors, it can be found that B16 melanoma can achieve good treatment results under light irradiation, with a significant reduction in tumor volume, while light irradiation cannot achieve the ablation treatment effect on A20 and SMMC-7721 tumors.

[0069] The above description is merely an optional embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A multi-wavelength photothermal and photodynamic laser therapy device for melanoma, characterized in that, include: case; At least one laser element and at least one fiber optic probe are located within the housing, wherein the laser element includes a first laser element, a second laser element, and a third laser element; The laser element is configured to emit a first wavelength laser, the second laser element is configured to emit a second wavelength laser, and the third laser element is configured to emit a third wavelength laser. The first wavelength laser is in the range of 750-1000nm and is used to stimulate the endogenous melanin in melanoma to produce a photothermal effect. The second wavelength laser is in the range of 1000-1350nm. The third wavelength laser is in the range of 600-750nm and is used to stimulate the endogenous melanin to produce a photodynamic effect. The laser therapy device is configured to achieve synergistic photothermal and photodynamic therapy using only the endogenous melanin without the application of an exogenous photosensitizer.

2. The multi-wavelength photothermal and photodynamic laser therapy device for melanoma as described in claim 1, characterized in that, The power density of the laser is 0.1-2 W / cm2, and the irradiation time is 5-20 min.

3. The multi-wavelength photothermal and photodynamic laser therapy device for melanoma as described in claim 2, characterized in that, The power density of the fiber optic probe is 0.5-1W / cm2, and the irradiation time is 5-10min.

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