Protein detection device and sample analyzer

Abstract

The application provides a protein detection device and a sample analyzer. The protein detection device comprises at least one protein detection channel; the protein detection channel comprises an emitter, a detection cell and a signal receiving amplification processing circuit; the emitter is used to provide a light source; the detection cell is used to perform a specific protein detection on a to-be-detected liquid in multiple specific protein detections by using the light source; the signal receiving amplification processing circuit is a gain-adjustable signal receiving amplification processing circuit, which is used to receive a feedback signal of the detection cell performing the selected specific protein detection, and select a corresponding gain coefficient according to the selected specific protein detection, so as to perform gain amplification and processing on the feedback signal based on the gain coefficient. In the case that the number of detection cells in the protein detection channel is reduced to one, the protein detection channel can still realize the detection of multiple specific proteins, and the structure of the device is simplified and the cost of the device is reduced.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and in particular to a protein detection device and a sample analyzer. Background Technology

[0002] With national medical policies regulating antibiotic use, healthcare professionals aim to differentiate between bacterial and viral infections early in the diagnosis process. C-reactive protein (CRP) and other specific proteins are considered primary indicators of bacterial or viral infections and are often recommended for bundled testing with CRP in outpatient settings. This has led to the development of new blood analysis devices capable of detecting both blood cells and CRP. However, the protein detection components in these devices can only detect CRP and cannot simultaneously measure other specific protein parameters besides CRP. Summary of the Invention

[0003] This application aims to at least solve one of the technical problems existing in the prior art. To this end, one objective of this application is to provide a protein detection device and a sample analyzer that, while reducing the number of detection cells in the first protein detection channel to one, can still achieve the detection of multiple specific proteins in the first protein detection channel, thereby simplifying the structure of the device and reducing the cost of the device.

[0004] To solve the above-mentioned technical problems, one technical solution adopted in this application is: providing a protein detection device, which includes: at least one protein detection channel; the protein detection channel includes: a transmitter, a detection cell, and a signal receiving, amplifying, and processing circuit; wherein, the transmitter is used to provide a light source; the detection cell is used to select one specific protein detection among multiple specific protein detections using the light source; wherein, the signal receiving, amplifying, and processing circuit is a gain-adjustable signal receiving, amplifying, and processing circuit, used to receive the feedback signal from the detection cell performing the selected specific protein detection, and select a corresponding gain coefficient according to the selected specific protein detection, so as to amplify and process the feedback signal based on the gain coefficient.

[0005] The signal receiving and amplification processing circuit includes a receiver, an amplification circuit, and a signal acquisition circuit. The receiver receives the feedback signal from the detection cell performing the detection of a selected specific protein and converts it into a corresponding electrical signal. The amplification circuit is connected to the receiver and is a gain-adjustable amplification circuit used to select the corresponding gain coefficient based on the selected specific protein detection and amplify the electrical signal based on the gain coefficient. The signal acquisition circuit is connected to the amplification circuit and is used to acquire the amplified electrical signal to obtain the parameter information of the selected specific protein.

[0006] The amplification circuit includes a single-stage gain amplifier circuit and a control circuit. The single-stage gain amplifier circuit is connected to the receiver and is used to amplify the converted electrical signal based on the gain coefficient corresponding to the selected specific protein detection. The control circuit is connected to the single-stage gain amplifier circuit and is used to control the single-stage gain amplifier circuit to operate with the gain coefficient corresponding to the selected specific protein detection. The signal acquisition circuit is an analog-to-digital conversion acquisition circuit, and the accuracy of the digital-to-analog conversion acquisition circuit is greater than the preset accuracy.

[0007] The amplification circuit includes a multi-stage gain amplifier circuit and a control circuit. The multi-stage gain amplifier circuit is connected to the receiver and includes multiple gain amplification units for multi-stage gain amplification of the converted electrical signal based on the gain coefficient corresponding to the selected specific protein detection. The control circuit is connected to the multi-stage gain amplifier circuit and controls the multi-stage gain amplifier circuit to operate with the gain coefficient corresponding to the selected specific protein detection. The signal acquisition circuit is an analog-to-digital conversion acquisition circuit, and the accuracy of the digital-to-analog conversion acquisition circuit is no greater than a preset accuracy.

[0008] The multiple gain amplification units include: a first-stage gain amplification unit and a second-stage gain amplification unit; the first-stage gain amplification unit is connected to the receiver and is used to amplify the electrical signal in the first stage with a first gain coefficient; the second-stage gain amplification unit is connected to the first-stage gain amplification unit and is used to amplify the electrical signal amplified by the first-stage gain amplification unit in the second stage with a second gain coefficient.

[0009] The signal receiving and amplification processing circuit further includes an I / V conversion circuit. The I / V conversion circuit is located between the receiver and the amplification circuit and is used to convert the current signal output by the receiver to obtain a voltage signal. The amplification circuit receives the voltage signal to select the corresponding gain coefficient according to the selected specific protein detection, and amplifies the voltage signal based on the gain coefficient.

[0010] The protein detection channel includes at least one protein detection channel: a first protein detection channel and a second protein detection channel; wherein the first protein detection channel and the second protein detection channel are used to perform different specific protein detections in the same test solution, the same specific protein detections in different test solutions, or the same specific protein detections in different test solutions, respectively.

[0011] The feedback signal is the transmitted light signal and / or the scattered light signal.

[0012] To solve the above-mentioned technical problems, another technical solution adopted in this application is to provide a sample analyzer. This sample analyzer includes the protein detection device as described above.

[0013] The sample analyzer also includes a complete blood count (CBC) testing device, which includes a CBC testing channel. The CBC testing device is used to provide a testing site for the test solution and to test the test solution to obtain testing information for at least one CBC parameter.

[0014] The protein detection device and sample analyzer provided in this application include at least one protein detection channel, which is configured to include an emitter to provide a light source. A detection cell is also included, allowing the detection cell to select one specific protein from multiple specific protein detections using the light source. Furthermore, a gain-adjustable signal receiving, amplifying, and processing circuit is included. This circuit receives the feedback signal from the detection cell performing the selected specific protein detection, selects a corresponding gain coefficient based on the selected specific protein detection, and amplifies and processes the feedback signal based on this gain coefficient to obtain the selected specific protein. The signal receiving and amplifying processing circuit can select the corresponding gain coefficient according to the specific protein detection selected in the detection cell, thus enabling the detection cell to detect different specific proteins. This allows the protein detection device to perform multiple specific protein detections even with only one detection cell in the protein detection channel, simplifying the device structure and reducing costs. Furthermore, the protein detection channel is not limited to detecting specific, fixed proteins but can detect any protein, thereby expanding the protein detection range of the sample analyzer and making the protein detection device more versatile, adaptable, and better suited to the needs of doctors and patients. Attached Figure Description

[0015] The above and / or additional aspects and advantages of this application will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0016] Figure 1 This is a schematic diagram of the protein detection device provided in the first embodiment of this application;

[0017] Figure 2 This is a schematic diagram of the protein detection device provided in the second embodiment of this application;

[0018] Figure 3 This is a schematic diagram of the protein detection device provided in the third embodiment of this application;

[0019] Figure 4 This is a schematic diagram of the protein detection device provided in the fourth embodiment of this application;

[0020] Figure 5 This is a schematic diagram of the protein detection device provided in the fifth embodiment of this application;

[0021] Figure 6 This is a schematic diagram of the protein detection device provided in the sixth embodiment of this application;

[0022] Figure 7 This is a schematic diagram of the protein detection device provided in the seventh embodiment of this application;

[0023] Figure 8 This is a schematic diagram of the sample analyzer provided in the first embodiment of this application;

[0024] Figure 9 This is a schematic diagram of the sample analyzer provided in the second embodiment of this application. Detailed Implementation

[0025] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0026] The terms "first," "second," and "third" in this application are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. All directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of this application are only used to explain the relative positional relationships and movements between components in a specific orientation (as shown in the figures). If the specific orientation changes, the directional indications also change accordingly. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or devices.

[0027] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0028] The present application will now be described in detail with reference to the accompanying drawings and embodiments.

[0029] Please see Figure 1 , Figure 1 This is a schematic diagram of the structure of the protein detection device provided in the first embodiment of this application; in this embodiment, a protein detection device 10 is provided. The protein detection device 10 includes at least one protein detection channel 11.

[0030] Specifically, the protein detection channel 11 may include a detection cell 111, a transmitter 112, and a signal receiving, amplifying, and processing circuit 113.

[0031] The transmitter 112 is used to provide a light source; the detection cell 111 is used to select one specific protein from multiple specific protein detections in the test solution using the light source; in a specific embodiment, the transmitter 112 and the signal receiving amplification and processing circuit 113 are disposed at both ends of the detection cell 111, and the optical path between the transmitter 112 and the signal receiving and processing circuit passes through the test solution in the detection cell 111 so as to perform the selected specific protein detection on the test solution using the light source through the detection cell 111.

[0032] The signal receiving, amplification, and processing circuit 113 is a gain-adjustable signal receiving, amplification, and processing circuit. This circuit receives the feedback signal from the detection cell 111 performing the selected specific protein detection, and selects a corresponding gain coefficient based on the selected specific protein detection. The feedback signal is then amplified and processed based on this gain coefficient. The feedback signal can be a transmitted light signal and / or a scattered light signal; and each specific protein detection corresponds to one gain coefficient. In specific embodiments, the gain coefficients corresponding to different specific protein detections may be the same or different.

[0033] It is understandable that, since different specific protein-antibody complexes absorb the light beam emitted by the transmitter 112 differently, the feedback signals received by the signal receiving amplification and processing circuit 113 are different. In a specific embodiment, the signal receiving amplification and processing circuit 113 can select the corresponding gain coefficient according to the selected specific protein detection, so as to amplify and process the feedback signal based on the gain coefficient to match the reaction characteristics of different specific proteins. This enables the detection cell 111 to detect different specific proteins, thereby enabling the protein detection device 10 to achieve non-simultaneous detection of multiple specific proteins even when the number of detection cells 111 in the protein detection channel 11 is reduced to one. This simplifies the structure of the device 10 and reduces the cost of the device 10.

[0034] It should be noted that the protein detection channel 11 can detect any specific protein, as long as the detection principle of the protein detection channel 11 can be used for the detection of any specific protein. However, the gain coefficient of the signal receiving amplification processing circuit 113 is not exactly the same for different specific protein detections, and needs to be set or configured in advance according to the gain coefficient corresponding to the selected specific protein detection.

[0035] The protein detection device 10 provided in this embodiment includes at least one protein detection channel 11, which includes an emitter 112 to provide a light source. Simultaneously, a detection cell 111 is provided, allowing the detection cell 111 to select one specific protein from multiple specific protein detections using the light source. Furthermore, a gain-adjustable signal receiving and amplifying processing circuit 113 is provided to receive the feedback signal from the detection cell 111 performing the selected specific protein detection. The circuit selects a corresponding gain coefficient based on the selected specific protein detection, amplifies the feedback signal based on the gain coefficient, and processes it to obtain the parameter information of the selected specific protein. Because the signal receiving and amplifying processing circuit 113 can select a corresponding gain coefficient based on the specific protein detection selected by the detection cell 111, the detection cell 111 can detect different specific proteins. Therefore, even with the number of detection cells 111 in the protein detection channel 11 reduced to one, the protein detection device 10 can still perform multiple specific protein detections, simplifying the structure of the device 10 and reducing its cost. Furthermore, the protein detection channel 11 is not limited to detecting specific, fixed proteins, but can detect any protein, thereby expanding the sample analyzer 100 (see below). Figure 8 The increased protein detection range makes the protein detection device 10 more widely applicable, more adaptable, and better suited to the needs of doctors and patients.

[0036] Please see Figure 2 , Figure 2 This is a schematic diagram of the protein detection device provided in the second embodiment of this application. In one embodiment, the signal receiving, amplifying, and processing circuit 113 specifically includes a receiver 1131, an amplifying circuit 1132, and a signal acquisition circuit 1133. The signal acquisition circuit 1133 may specifically be an analog-to-digital converter acquisition circuit.

[0037] The receiver 1131 is used to receive the feedback signal from the detection cell 111 when performing the detection of the selected specific protein and convert it into a corresponding electrical signal. In a specific embodiment, the receiver 1131 can be a photodiode. Of course, in other specific embodiments, the receiver 1131 can be other instruments that can convert light signals into electrical signals.

[0038] The amplifier circuit 1132 is connected to the receiver 1131. In a specific embodiment, the amplifier circuit 1132 is a gain-adjustable amplifier circuit. The amplifier circuit 1132 is used to select the corresponding gain coefficient according to the selected specific protein detection, and amplify the electrical signal based on the gain coefficient. Before the chip processes the electrical signal, the amplifier circuit 1132 amplifies the electrical signal converted by the receiver 1131. This not only allows the protein detection device 10 to achieve the detection of multiple specific proteins even with the number of detection pools 111 in the protein detection channel 11 reduced to one, simplifying the structure of the device 10 and reducing its cost, but also ensures that the amplified electrical signal remains within the detection accuracy range of the processing chip, effectively improving the accuracy of the detection results.

[0039] The signal acquisition circuit 1133 is used to acquire the amplified electrical signal to obtain the parameter information of the selected specific protein based on the amplified electrical signal.

[0040] In one specific embodiment, see Figure 3 , Figure 3 This is a schematic diagram of the protein detection device provided in the third embodiment of this application; the amplification circuit 1132 includes a single-stage gain amplification circuit 1132a and a control circuit 1132b; wherein, the single-stage gain amplification circuit 1132a is connected to the receiver 1131 and is used to amplify the converted electrical signal based on the gain coefficient corresponding to the selected specific protein detection; the control circuit 1132b is connected to the single-stage gain amplification circuit 1132a and is used to control the single-stage gain amplification circuit 1132a to operate with the gain coefficient corresponding to the selected specific protein detection.

[0041] It is understood that single-stage gain amplification means that only one stage of gain amplification is performed on the converted electrical signal; however, the gain coefficient of this single-stage gain amplification can be adjusted and changed by the control circuit 1132b according to the selected specific protein detection, so that the detection cell 111 corresponding to this embodiment can also perform non-simultaneous detection of multiple different specific proteins; wherein, since the gain amplification circuit 1132 corresponding to this embodiment is a single-stage amplification, compared with the multi-stage gain amplification circuit 1132c, the number of amplification stages for the feedback signal of the selected specific protein detection performed by the detection cell 111 is less. Therefore, in a specific embodiment, the accuracy of the digital-to-analog conversion acquisition circuit can be greater than the preset accuracy to improve the detection accuracy; specifically, the digital-to-analog conversion acquisition circuit can be a 24-bit high-precision ADC chip.

[0042] In another specific embodiment, see Figure 4 , Figure 4This is a schematic diagram of the structure of the protein detection device 10 provided in the fourth embodiment of this application; the amplification circuit 1132 may include a multi-stage gain amplification circuit 1132c and a control circuit 1132d; wherein, the multi-stage gain amplification circuit 1132c is connected to the receiver 1131; in a specific embodiment, the multi-stage gain amplification circuit 1132c includes multiple gain amplification units, and the multiple gain amplification circuits 1132 are used to perform multi-stage gain amplification on the converted electrical signal based on the gain coefficient corresponding to the selected specific protein detection; the control circuit 1132d is connected to the multi-stage gain amplification circuit 1132c and is used to control the multi-stage gain amplification circuit 1132c to operate with the gain coefficient corresponding to the selected specific protein detection.

[0043] It is understood that multi-stage gain amplification refers to performing one, two, or three stages of gain amplification on the converted electrical signal; and the gain coefficient of each stage of gain amplification can be adjusted by the control circuit 1132d according to the selected specific protein detection, so that the detection cell 111 corresponding to this embodiment can also perform non-simultaneous detection of multiple different specific proteins; wherein, since the gain amplification circuit corresponding to this embodiment is multi-stage amplification, compared with the single-stage gain amplification circuit 1132a, the number of amplification stages for the feedback signal of the selected specific protein detection performed by the detection cell 111 is more. Therefore, in a specific embodiment, the accuracy of the digital-to-analog conversion acquisition circuit can be made not greater than the preset accuracy, so as to reduce costs.

[0044] In one embodiment, see Figure 5 , Figure 5 This is a schematic diagram of the protein detection device provided in the fifth embodiment of this application. Specifically, the multiple gain amplification units can be two gain amplification units: a first-stage gain amplification unit c1 and a second-stage gain amplification unit c2. The first-stage gain amplification unit c1 is connected to the receiver 1131 and is used to amplify the electrical signal using a first gain coefficient. The second-stage gain amplification unit c2 is connected to the first-stage gain amplification unit c1 and is used to amplify the amplified electrical signal using a second gain coefficient. Specifically, the first-stage gain amplification coefficient and the second-stage gain amplification coefficient can be adjusted by the control circuit 1132d according to the gain coefficient corresponding to the selected specific protein detection, so as to perform non-simultaneous detection of different specific proteins.

[0045] Before the chip processes the electrical signal, this application amplifies the electrical signal converted by the receiver 1131 through the amplifier circuit 1132. This not only enables the protein detection device 10 to detect multiple specific proteins even when the number of detection pools 111 in the protein detection channel 11 is reduced to one, but also simplifies the structure of the device 10, reduces the cost of the device 10, and effectively improves the accuracy of the detection results by keeping the amplified electrical signal within the detection accuracy range of the processing chip.

[0046] In one embodiment, please refer to Figure 6 , Figure 6 This is a schematic diagram of the protein detection device provided in the sixth embodiment of this application; the signal receiving and amplifying processing circuit 113 further includes an I / V conversion circuit 1134; the I / V conversion circuit 1134 is specifically disposed between the receiver 1131 and the amplification circuit 1132, and is used to convert the current signal output by the receiver 1131 to obtain a voltage signal; in a specific embodiment, the amplification circuit 1132 receives the voltage signal converted by the I / V conversion circuit 1134 to select the corresponding gain coefficient according to the selected specific protein detection, and amplifies the voltage signal based on the gain coefficient; the signal acquisition circuit 1133 is specifically used to acquire the amplified voltage signal to obtain the parameter information of the selected specific protein based on the amplified voltage signal.

[0047] In one embodiment, please refer to Figure 7 , Figure 7 This is a schematic diagram of the structure of the protein detection device provided in the seventh embodiment of this application; at least one protein detection channel 11 includes: a first protein detection channel 11A and a second protein detection channel 11B.

[0048] The first protein detection channel 11A and the second protein detection channel 11B are used to perform detection of different specific proteins in the same test solution, detection of different specific proteins in different test solutions, or detection of the same specific protein in different test solutions, so as to effectively improve the detection speed.

[0049] Please refer to the protein detection channel 11 in the above embodiment for the structure of the first protein detection channel 11A and the second protein detection channel 11B.

[0050] Unlike existing technologies, the protein detection device 10 of this application includes a first protein detection channel 11A and a second protein detection channel 11B. In practical applications, different blood samples and the same specific protein antibody reagent can be added to the detection pool 111 of the first protein detection channel 11A and the detection pool 111 of the second protein detection channel 11B. The first protein detection channel 11A and the second protein detection channel 11B emit light beams of the same intensity as light sources to achieve rapid detection of the same specific protein in different test solutions. Alternatively, the same blood sample and different specific protein antibody reagents can be added to the detection pool 111 of the first protein detection channel 11A and the detection pool 111 of the second protein detection channel 11B. The protein detection device 10 uses light beams of different intensities emitted by the first protein detection channel 11A and the second protein detection channel 11B as light sources to detect different specific proteins in the same test solution. Different blood samples and different specific protein antibody reagents can also be added to the detection pools 111 of the first protein detection channel 11A and the second protein detection channel 11B. The first protein detection channel 11A and the second protein detection channel 11B emit light beams of different intensities as light sources to detect different specific proteins in different test solutions. This allows for simultaneous detection by multiple protein detection channels 11, shortening the detection time for multiple blood samples or multiple tests on a single blood sample. It should be noted that the protein detection device 10 is not limited to detecting specific, fixed proteins, but can detect any first or second specific protein, thereby expanding the specific protein detection range of the sample analyzer, making the protein detection device 10 more versatile, adaptable, and better suited to the needs of doctors and patients.

[0051] In one embodiment, the first specific protein is serum amyloid A protein (SAA), and the second specific protein is C-reactive protein (CRP).

[0052] C-reactive protein (CRP) and serum amyloid A protein (SAA) are early inflammatory markers of infectious diseases, and are crucial for the diagnosis and assessment of the degree of inflammation in bacterial, viral, and tissue infections. CRP is an acute-phase reactant protein synthesized by the liver. As an acute-phase reactant, it rises within hours after bacterial infection and reaches its peak within 48 hours. A CRP concentration greater than 10 mg / L strongly suggests bacterial infection.

[0053] Serum amyloid A protein (SAA) is a precursor to tissue amyloid A and is an acute-phase reactive protein. Serum SAA is a normal component present in the blood at low levels. It rises rapidly during viral or bacterial infections, active lesions, or extensive tissue damage. It rises rapidly and significantly during acute viral infections (48-72 hours) and declines rapidly during the recovery period. Therefore, serum SAA is a highly effective auxiliary diagnostic indicator for viral infections, and its short half-life of only 50 minutes allows it to effectively and promptly reflect the severity of the disease.

[0054] The working principle of the protein detection device 10 will be explained in detail below with a specific embodiment.

[0055] Taking SAA protein detection and CRP protein detection as examples; where gain coefficient k1 corresponds to SAA protein detection and gain coefficient k2 corresponds to CRP protein detection; if detection cell 111 is selected to perform SAA protein detection, then blood sample and SAA antibody reagent are added to detection cell 111, transmitter 112 emits light source, and the feedback signal of SAA protein detection performed by detection cell 111 using light source is received by receiver 1131, and receiver 1131 converts the feedback signal to obtain a corresponding current signal, which is received by I / V conversion circuit 1134 and converted to obtain a corresponding voltage signal; control circuit 1132b controls single-stage gain amplifier circuit 1132a or control circuit 1132d controls multi-stage gain amplifier circuit 1132c to work with gain coefficient k1, so as to amplify the voltage signal through single-stage gain amplifier circuit 1132a or multi-stage gain amplifier circuit 1132c; then the signal acquisition circuit 1133 acquires the amplified voltage signal. The SAA protein parameter information is then obtained from the amplified voltage signal. If the detection cell 111 is selected to perform CRP protein detection, blood sample and CRP antibody reagent are added to the detection cell 111. The transmitter 112 emits a light source, and the feedback signal of the detection cell 111 performing CRP protein detection using the light source is received by the receiver 1131. The receiver 1131 converts the feedback signal to obtain a corresponding current signal, which is received by the I / V conversion circuit 1134 and converted to obtain a corresponding voltage signal. The control circuit 1132b controls the single-stage gain amplifier circuit 1132a or the control circuit 1132d controls the multi-stage gain amplifier circuit 1132c to operate with a gain coefficient k2, so as to amplify the voltage signal through the single-stage gain amplifier circuit 1132a or the multi-stage gain amplifier circuit 1132c. Then, the signal acquisition circuit 1133 acquires the amplified voltage signal, and the CRP protein parameter information is obtained from the amplified voltage signal.

[0056] Please see Figure 8 , Figure 8 This is a schematic diagram of the structure of the sample analyzer provided in the first embodiment of this application; in this embodiment, a sample analyzer 100 is provided; the sample analyzer 100 includes a protein detection device, which may be the protein detection device 10 provided in any of the above embodiments.

[0057] like Figure 8 As shown, the sample analyzer 100 of this embodiment further includes a routine blood count (RBC) testing device 20, which includes a RBC testing channel. The RBC testing channel provides a testing site for the test solution and performs testing on the test solution to obtain detection information for at least one RBC parameter.

[0058] The protein detection device 10 can operate in parallel with the blood routine testing device 20, or it can operate in a non-parallel manner. Parallel operation means that the protein detection device 10 and the blood routine testing device 20 can operate simultaneously to detect their respective relevant parameters. Non-parallel operation means that the protein detection device 10 and the blood routine testing device 20 can operate at different times to detect their respective relevant parameters.

[0059] Please see Figure 8 The sample analyzer 100 in this embodiment further includes a controller 30. The controller 30 is configured to, in response to a mode switching command, control the blood routine detection channel to perform blood routine detection on the test solution, and / or control the first protein detection channel 11A to perform first specific protein detection on the test solution or control the second protein detection channel 11B to perform second specific protein detection on the test solution.

[0060] Unlike existing technologies, the sample analyzer 100 of this application includes a protein detection device 10 and a blood routine test device 20. The protein detection device 10 includes a first protein detection channel 11A and a second protein detection channel 11B. In this way, the detection of a first specific protein or a second specific protein can be performed simultaneously with the blood routine test, shortening the test time. In addition, the protein detection device 10 is not limited to detecting specific, fixed specific proteins, but can detect any specific protein, thereby expanding the specific protein detection range of the sample analyzer 100, making the sample analyzer 100 more versatile, more adaptable, and better meeting the needs of doctors and patients.

[0061] Unlike existing technologies, the sample analyzer 100 of this application includes a protein detection device 10 and a blood routine test device 20. The protein detection device 10 includes a first protein detection channel 11A and a second protein detection channel 11B. In this way, when performing a blood routine test, the first specific protein and / or the second specific protein can be detected simultaneously, shortening the test time. In addition, the protein detection device 10 is not limited to detecting specific, fixed specific proteins, but can detect any specific protein, thereby expanding the specific protein detection range of the sample analyzer 100, making the sample analyzer 100 more versatile, more adaptable, and better meeting the needs of doctors and patients.

[0062] In some embodiments, see Figure 9 , Figure 9 This is a schematic diagram of the sample analyzer provided in the second embodiment of this application; the sample analyzer 100 further includes a sampling unit 50 and a reagent unit 60. The sample analyzer 100 is provided with several cells, such as a first detection cell 111, a second detection cell 121, and a blood routine detection cell 211. The sampling unit 50 is configured to aspirate a sample, wherein the sample can be a blood sample, which may contain serum or plasma. The reagent unit 60 is configured to dispense processing reagents into the several cells. The several cells are configured to contain a test solution, wherein the test solution is the liquid resulting from the reaction of the sample with the processing reagents.

[0063] The complete blood count chamber 211 can be a white blood cell reaction chamber and / or a red blood cell counting chamber. The white blood cell reaction chamber is used for reacting the blood sample with a hemolytic agent. The red blood cell counting chamber is used to count the red blood cells and platelets in the blood sample.

[0064] In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be through some interfaces, or indirect coupling or communication connection between apparatuses or units, and may be electrical, mechanical, or other forms.

[0065] Furthermore, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0066] The above are merely embodiments of this application and do not limit the scope of this patent application. Any equivalent structural or procedural changes made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the scope of patent protection of this application.

Claims

1. A protein detection device, characterized in that, include: At least one protein detection channel; The protein detection channel includes: Emitter, used to provide a light source; The detection cell is used to select one specific protein from multiple specific protein detections using the light source; different specific protein-antibody complexes absorb the light beam emitted by the emitter differently; A signal receiving, amplifying, and processing circuit, wherein the signal receiving, amplifying, and processing circuit is a gain-adjustable signal receiving, amplifying, and processing circuit, used to receive the feedback signal from the detection cell performing the selected specific protein detection, and select a corresponding gain coefficient according to the selected specific protein detection, so as to amplify and process the feedback signal based on the gain coefficient; wherein, different specific protein detections correspond to different gain coefficients. The signal receiving and amplification processing circuit includes: A receiver is used to receive the feedback signal from the detection pool when performing the detection of the selected specific protein and convert it into a corresponding electrical signal. An amplifier circuit is connected to the receiver, wherein the amplifier circuit is a gain-adjustable amplifier circuit, used to select the corresponding gain coefficient according to the selected specific protein detection, and to amplify the electrical signal based on the gain coefficient; A signal acquisition circuit, connected to the amplification circuit, is used to acquire the amplified electrical signal to obtain parameter information of the selected specific protein based on the amplified electrical signal. The amplifier circuit includes: A multi-stage gain amplifier circuit is connected to the receiver, wherein the multi-stage gain amplifier circuit includes multiple gain amplification units for performing multi-stage gain amplification on the converted electrical signal based on the gain coefficient corresponding to the selected specific protein detection; the multiple gain amplification units are connected in series. A control circuit, connected to the multi-stage gain amplifier circuit, is used to control the multi-stage gain amplifier circuit to operate with the gain coefficient corresponding to the selected specific protein detection. Alternatively, the amplifier circuit includes: A single-stage gain amplifier circuit, connected to the receiver, is used to amplify the converted electrical signal based on the gain coefficient corresponding to the selected specific protein detection. A control circuit, connected to the single-stage gain amplifier circuit, is used to control the single-stage gain amplifier circuit to operate with the gain coefficient corresponding to the selected specific protein detection. The signal receiving and amplification processing circuit further includes: an I / V conversion circuit disposed between the receiver and the amplification circuit, used to convert the current signal output by the receiver to obtain a voltage signal, wherein the amplification circuit receives the voltage signal to select the corresponding gain coefficient according to the selected specific protein detection, and amplifies the voltage signal based on the gain coefficient; The signal acquisition circuit is an analog-to-digital conversion acquisition circuit. When the amplification circuit includes the single-stage gain amplifier circuit, the accuracy of the analog-to-digital conversion acquisition circuit is greater than the preset accuracy, so as to improve the detection accuracy by combining the single-stage gain amplifier circuit with the high-precision analog-to-digital conversion acquisition circuit. The analog-to-digital conversion acquisition circuit is a 24-bit high-precision ADC chip. When the amplification circuit includes the multi-stage gain amplifier circuit, the accuracy of the analog-to-digital conversion acquisition circuit is not greater than the preset accuracy.

2. The protein detection device according to claim 1, characterized in that, The plurality of gain amplification units include: The first-stage gain amplification unit is connected to the receiver and is used to amplify the electrical signal with a first gain coefficient. The second-stage gain amplification unit is connected to the first-stage gain amplification unit and is used to perform second-stage gain amplification on the electrical signal after gain amplification by the first-stage gain amplification unit with a second gain coefficient.

3. The protein detection device according to claim 1, characterized in that, The at least one protein detection channel includes: a first protein detection channel and a second protein detection channel; The first protein detection channel and the second protein detection channel are respectively used to perform the detection of different specific proteins in the same test solution, the detection of the same specific protein in different test solutions, or the detection of different specific proteins in different test solutions.

4. The protein detection device according to any one of claims 1-3, characterized in that, The feedback signal is a transmitted light signal and / or a scattered light signal.

5. A sample analyzer, characterized in that, include: The protein detection device as described in any one of claims 1-4.

6. The sample analyzer according to claim 5, characterized in that, The sample analyzer also includes: a routine blood test device, which includes a routine blood test channel; The blood routine testing channel is used to provide a testing site for the test solution and to test the test solution to obtain testing information for at least one blood routine parameter.

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