Cosmetic liposome composition

Abstract

Provided is a cosmetic liposome composition containing liposomes with an increased content of a cosmetic ingredient. A cosmetic liposome composition containing: liposomes containing a polyoxyethylene phytostanol, lecithin, and at least one of cholesterol and a phytostanol other than the polyoxyethylene phytostanol, and having an average particle diameter of 100 nm to 350 nm; an inner aqueous phase containing water, ethanol, and a cosmetic ingredient, and contained in the liposomes; and an outer aqueous phase containing water and ethanol, and in which the liposomes are dispersed.

Description

Technical Field

[0001] This invention relates to a liposome composition for cosmetic use. Background Technology

[0002] Liposomes are closed endoplasmic reticulum structures formed by lipid bilayers containing lipids such as lecithin, and they contain an aqueous phase (internal aqueous phase) within the space of the closed vesicles. Furthermore, liposomes typically exist as an aqueous solution dispersed outside the closed vesicles (external aqueous phase).

[0003] In recent years, the applicability of liposomes containing pharmaceutical or cosmetic ingredients in the internal aqueous phase in pharmaceutical compositions or cosmetics has been studied.

[0004] For example, Patent Document 1 proposes a liposome composition containing liposomes, which contain pharmaceutical ingredients in an internal aqueous phase and have an average particle size of less than 5 nm to 100 nm.

[0005] Furthermore, Patent Document 2 proposes a cosmetic liposome composition containing liposomes, which contain oil components in the internal aqueous phase and have an average particle size of less than 82 nm.

[0006] Furthermore, Patent Document 3 proposes a cosmetic liposome composition containing liposomes, which contain cosmetic ingredients in an internal aqueous phase and have an average particle size of less than 50 nm.

[0007] Furthermore, Patent Document 4 proposes a cosmetic composition comprising a disc-shaped twin-cell structure formed by a lipid bilayer membrane, rather than liposomes.

[0008] Furthermore, Patent Document 5 proposes a cosmetic product containing vesicles contained in an aqueous phase containing ethanol.

[0009] Patent Document 1: Japanese Patent No. 6251385

[0010] Patent Document 2: Japanese Patent Application Publication No. 2020-93992

[0011] Patent Document 3: Japanese Patent No. 5064717

[0012] Patent Document 4: International Publication No. 2017 / 090740

[0013] Patent Document 5: Japanese Patent Application Publication No. 2016-56198

[0014] When applying liposomes containing beauty ingredients to cosmetics, there is a need for liposomes that can contain a larger amount of beauty ingredients.

[0015] In this study, the inventors have made a new discovery that the liposomes contained in the compositions proposed in Patent Documents 1-3, the twin-cell structure contained in the composition proposed in Patent Document 4, and the vesicles contained in the composition proposed in Patent Document 5 have room for improvement in terms of the content of cosmetic ingredients.

[0016] Furthermore, from the perspective of the content of cosmetic ingredients, it is desirable that the proportion of liposomes (single-layer vesicles) with a single-layer structure having a lipid bilayer membrane as one layer is high among the liposomes contained in the liposome composition.

[0017] The inventors also discovered that the compositions in Patent Documents 2, 3 and 5 have a low proportion of liposomes with a monolayer structure and insufficient content of cosmetic ingredients. Summary of the Invention

[0018] The present invention was made in view of the above circumstances, and the problem it seeks to solve is to provide a cosmetic liposome composition containing liposomes with an increased content of beauty ingredients.

[0019] <1> A cosmetic liposome composition comprising: liposomes containing polyoxyethylene phytosterol, lecithin, cholesterol, and at least one of phytosterols other than the aforementioned polyoxyethylene phytosterol, wherein the average particle size of the liposomes is 100 nm to 350 nm; an inner aqueous phase containing water, ethanol, and cosmetic ingredients, and contained within the aforementioned liposomes; and an outer aqueous phase containing water and ethanol, wherein the aforementioned liposomes are dispersed.

[0020] <2> According to the cosmetic liposome composition described in <1> above, the average molar number of oxyethylene addition of the polyoxyethylene phytosterol is 10 or more.

[0021] <3> The cosmetic liposome composition according to <1> or <2>, wherein the content of the above-mentioned polyoxyethylene phytosterol relative to the cosmetic liposome composition is 0.1% by mass or more.

[0022] <4> The cosmetic liposome composition according to any one of <1> to <3>, wherein the sum of the content of ethanol in the inner aqueous phase and the content of ethanol in the outer aqueous phase relative to the total mass of the cosmetic liposome composition is 0.2% to 30% by mass.

[0023] <5> The cosmetic liposome composition according to any one of <1> to <4>, wherein the content of at least one of the cholesterol and phytosterols other than the polyoxyethylene phytosterol is 0.1% to 0.5% by mass relative to the total mass of the cosmetic liposome composition.

[0024] <6> A cosmetic liposome composition according to any one of <1> to <5>, wherein the cosmetic ingredient is a water-soluble cosmetic ingredient.

[0025] <7> The cosmetic liposome composition according to any one of <1> to <6>, wherein the cosmetic ingredient is at least one selected from caffeine, pyridoxine hydrochloride and niacinamide.

[0026] <8> The cosmetic liposome composition according to any one of <1> to <7>, wherein the total number of liposomes having a monolayer structure is 70% or more relative to the total number of liposomes contained in the cosmetic liposome composition.

[0027] <9> A cosmetic product comprising any one of <1> to <8> cosmetic liposome composition.

[0028] Invention Effects

[0029] According to the present invention, a cosmetic liposome composition containing liposomes with an increased content of cosmetic ingredients can be provided. Attached Figure Description

[0030] Figure 1 The images show TEM (Transmission Electron Microscope) images of the cosmetic liposome compositions obtained in Examples 1-2.

[0031] Figure 2 TEM image showing the cosmetic liposome composition obtained in Example 3-1.

[0032] Figure 3 TEM images showing the cosmetic liposome compositions obtained in Examples 3-2.

[0033] Figure 4 TEM images showing the cosmetic liposome compositions obtained in Comparative Examples 1-2.

[0034] Figure 5 TEM images showing the cosmetic liposome compositions obtained in Comparative Examples 1-5. Detailed Implementation

[0035] The embodiments of the present invention will now be described in detail. However, the present invention is not limited to the embodiments described below. In the embodiments described below, except as specifically stated, the constituent elements are not essential. The same applies to numerical values ​​and their ranges, and they do not limit the present invention.

[0036] In this invention, the numerical range represented by “~” includes the numerical values ​​recorded before and after “~”, which are respectively the minimum and maximum values.

[0037] In the numerical ranges described in stages in this invention, the upper or lower limit value described in one numerical range can be replaced with the upper or lower limit value of other numerical ranges described in stages. Furthermore, the upper or lower limit value of the numerical range described in this invention can also be replaced with the value shown in the embodiments.

[0038] In this invention, each component may contain multiple corresponding compounds. When multiple substances corresponding to each component are present in the composition, unless otherwise specified, the content rate of each component represents the total content rate of the multiple substances present in the composition.

[0039] In this invention, "liposomes" are closed endoplasmic reticulum formed by lipid bilayer membranes.

[0040] A lipid bilayer structure consisting of one layer is called a monolayer structure, while a lipid bilayer structure consisting of multiple layers is called a multilayer structure.

[0041] In this invention, "internal aqueous phase" refers to a solution existing within a closed endoplasmic reticulum.

[0042] In this invention, the "external aqueous phase" refers to the solution in which the liposomes are dispersed.

[0043] In this invention, "cholesterol" refers to the general term for sterols contained in animals, and "phytosterols" refers to the general term for sterols contained in plants.

[0044] In this invention, "beauty ingredient" refers to an ingredient that acts on the skin and other parts of the body, and indicates an effective ingredient used in cosmetics and the like.

[0045] Furthermore, "water solubility" refers to a solubility of 0.1g or more in 100g of water at a liquid temperature of 22℃ and a pH of 7.0.

[0046] In this invention, "average particle size" refers to the volume average particle size obtained by dynamic light scattering method.

[0047] Examples of dynamic light scattering particle size analyzers include the FPAR-1000 thick particle size analyzer (manufactured by Otsuka Electronics Co., Ltd.), the Nanotrac UPA (manufactured by Nikkiso Co., Ltd.), and the LB-550 dynamic light scattering particle size distribution measuring device (manufactured by HORIBA, Ltd.).

[0048] The cosmetic liposome composition was diluted with pure water to 10 times its mass, and the average particle size was determined at room temperature (e.g., 25°C).

[0049] In addition, "pure water" refers to pure water obtained through ultrapure water manufacturing equipment manufactured by the Merck Group.

[0050] In this invention, the "ratio of the total number of liposomes with a monolayer structure to the total number of liposomes contained in the cosmetic liposome composition" is determined by observing the liposomes contained in the cosmetic liposome composition using a transmission electron microscope (TEM) under frozen conditions, and counting the total number of liposomes contained in the cosmetic liposome composition and the total number of liposomes with a monolayer structure.

[0051] More specifically, firstly, a TEM image of the cosmetic liposome composition was obtained at 50,000x magnification using a transmission electron microscope.

[0052] At any three points in the TEM image, the total number of liposomes present within a circle with a radius of 2 μm and the total number of liposomes with a monolayer structure are counted respectively, thereby determining the "ratio of the total number of liposomes with a monolayer structure to the total number of liposomes contained in the cosmetic liposome composition".

[0053] Furthermore, in this invention, "the ratio of the total number of liposomes having a monolayer structure to the total number of liposomes contained in the cosmetic liposome composition" is the average of three points.

[0054] In this invention, the content of cosmetic ingredients in liposomes can be evaluated by the "average particle size of liposomes" and the "ratio of the total number of liposomes with a monolayer structure to the total number of liposomes contained in the cosmetic liposome composition".

[0055] (Liposome composition for cosmetic use)

[0056] The cosmetic liposome composition of the present invention comprises: liposomes containing polyoxyethylene phytosterol, lecithin, cholesterol, and at least one of phytosterols other than the aforementioned polyoxyethylene phytosterol, and having an average particle size of 100 nm to 350 nm; an inner aqueous phase containing water, ethanol, and cosmetic ingredients, and contained within the aforementioned liposomes; and an outer aqueous phase containing water and ethanol, and dispersing the aforementioned liposomes.

[0057] The cosmetic liposome composition according to the present invention provides a cosmetic liposome composition containing liposomes with excellent content of beauty ingredients.

[0058] The reasons for the aforementioned effects are speculated as follows, but are not limited to these.

[0059] It is speculated that in the cosmetic liposome composition of the present invention, the liposomes contain polyoxyethylene phytosterol, which hinders the approach of the lipid bilayer membranes to each other, and the polyoxyethylene phytosterol helps the formation of liposomes. Therefore, the average particle size of the formed liposomes and the proportion of liposomes with monolayer structure are increased, and the content of cosmetic ingredients is improved.

[0060] From the viewpoint of increasing the content of cosmetic ingredients, in the liposome composition for cosmetic use, the ratio of the content of polyoxyethylene phytosterol to the content of ethanol (content of polyoxyethylene phytosterol / content of ethanol) is preferably 0.001 to 1, more preferably 0.002 to 0.98, and even more preferably 0.03 to 0.97, based on a mass basis.

[0061] Furthermore, by keeping the ratio of polyoxyethylene phytosterol content to ethanol content within the aforementioned range, it is possible to inhibit the aggregation of liposomes over time, the increase in liposome particle size, and the suspension of the cosmetic liposome composition.

[0062] Furthermore, by keeping the ratio of polyoxyethylene phytosterols to ethanol within the aforementioned range, it is possible to inhibit the aggregation and multilayering of condensed liposomes, thereby reducing the content of cosmetic ingredients.

[0063] Hereinafter, the above-mentioned ability to inhibit liposome aggregation will be referred to as time-dependent stability.

[0064] In addition, the ratio of the content of polyoxyethylene phytosterols to the content of ethanol in the cosmetic liposome composition is rounded to the fourth decimal place.

[0065] In the liposome composition for cosmetic use, the ratio of the content of polyoxyethylene phytosterol to the content of cholesterol (content of polyoxyethylene phytosterol / content of cholesterol) is preferably 2 or less, more preferably 1.7 or less, and even more preferably 1 or less, based on a mass basis.

[0066] By increasing the proportion of liposomes with a monolayer structure in the cosmetic liposome composition by having a ratio of polyoxyethylene phytosterols to cholesterol of less than 2 on a mass basis, the content of beauty ingredients is further improved.

[0067] Furthermore, there is no particular limitation on the lower limit of the ratio of polyoxyethylene phytosterol content to cholesterol content in the cosmetic liposome composition, but from the viewpoint of liposome formation, it is preferably 0.1 or more.

[0068] In addition, the ratio of the content of polyoxyethylene phytosterols to the content of cholesterol in the cosmetic liposome composition is rounded to the second decimal place.

[0069] (liposomes)

[0070] In the cosmetic liposome composition of the present invention, the liposomes have an average particle size of 100 nm to 350 nm.

[0071] By using liposomes with an average particle size of 100 nm or more, the content of cosmetic ingredients is improved. Furthermore, by using liposomes with an average particle size of 100 nm or more, the proportion of liposomes with a monolayer structure in cosmetic liposome compositions tends to increase further.

[0072] Liposomes with an average particle size exceeding 350 nm exhibit low stability over time and tend to multiply due to aggregation and agglomeration, resulting in a decrease in the content of cosmetic ingredients. By setting the average particle size of liposomes to 350 nm or less, this multiplying can be suppressed. Furthermore, when the average particle size of liposomes exceeds 350 nm, the turbidity of the manufactured cosmetic liposome composition tends to increase. By setting the average particle size of liposomes to 350 nm or less, the increase in turbidity of the cosmetic liposome composition can be suppressed, resulting in a superior appearance. Moreover, by setting the average particle size of liposomes to 350 nm or less, the permeability of the cosmetic liposome composition to the skin and the like can be improved.

[0073] From the perspective of improving the content of beauty ingredients and the stability over time, the average particle size of liposomes is preferably 110nm to 330nm, more preferably 120nm to 320nm, and even more preferably 150nm to 300nm.

[0074] From the viewpoint of increasing the content of cosmetic ingredients, liposomes are preferably liposomes with a monolayer structure (also known as monolayer vesicles), and the total number of liposomes with a monolayer structure is preferably 50% or more, more preferably 70% or more, relative to the total number of liposomes contained in the liposome composition.

[0075] In addition, liposome compositions may also contain liposomes with a multilayer structure (also known as multilayer endoplasmic reticulum).

[0076] Liposomes contain polyoxyethylene phytosterols, lecithin, cholesterol, and at least one of the following phytosterols other than polyoxyethylene phytosterols:

[0077] More specifically, the lipid bilayer membrane that makes up liposomes contains the above-mentioned components. The components are described below.

[0078] (Polyoxyethylene phytosterols)

[0079] Polyoxyethylene phytosterols are substances composed of (CH2CH2-O) n The term refers to oxyvinyl phytosterols.

[0080] In this invention, phytosterols include hydrogenated phytosterols (phytosterols).

[0081] Specific examples of phytosterols include sitosterol, stigmasterol, fucosterol, spinasterol, brassosterol, and their hydrogenated derivatives.

[0082] From the viewpoint of increasing the content of beauty ingredients, the average molar number of oxyethylene addition of polyoxyethylene phytosterols is preferably 5 or more, more preferably 10 or more, and even more preferably 20 or more.

[0083] Furthermore, by setting the average molar addition of oxyvinyl groups of polyoxyethylene phytosterols to 5 or more, the proportion of liposomes with a monolayer structure in the cosmetic liposome composition can be increased, thereby further improving the content of cosmetic ingredients in the liposomes. The average molar addition of oxyvinyl groups of polyoxyethylene phytosterols can be referenced from the catalog values.

[0084] Examples of commercially available polyoxyethylene phytosterols include Nikkol (registered trademark) BPS-5 (average molar addition of vinyl oxy group 5), Nikkol (registered trademark) BPS-10 (average molar addition of vinyl oxy group 10), Nikkol (registered trademark) BPS-20 (average molar addition of vinyl oxy group 20), Nikkol (registered trademark) BPS-30 (average molar addition of vinyl oxy group 30), and Nikkol (registered trademark) BPSH-25 (average molar addition of vinyl oxy group 25, an ethylene oxide adduct of hydrogenated phytosterol), manufactured by Nikkol Chemicals Co., Ltd., and NIHON EMULSION. EMLEX (registered trademark) manufactures PS-5 (average molar addition of vinyl oxytoluene 5), PS-10 (average molar addition of vinyl oxytoluene 10), PS-20 (average molar addition of vinyl oxytoluene 20), PS-30 (average molar addition of vinyl oxytoluene 30), and PS-25 (average molar addition of vinyl oxytoluene 25), etc.

[0085] The content of polyoxyethylene phytosterol relative to the total mass of the cosmetic liposome composition is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and even more preferably 0.23% by mass or more.

[0086] By increasing the content of polyoxyethylene phytosterols to 0.05% by mass or more, the proportion of liposomes with a monolayer structure in cosmetic liposome compositions can be increased, thereby further improving the content of cosmetic ingredients in liposomes.

[0087] Furthermore, the content of polyoxyethylene phytosterol relative to the total mass of the cosmetic liposome composition is preferably 1% by mass or less, more preferably 0.70% by mass or less. By having a content of polyoxyethylene phytosterol of 1% by mass or less, the formation of disc particles or micelles that replace liposomes can be suppressed.

[0088] (Lecithin)

[0089] Examples of lecithin include natural lecithin obtained from plants such as soybeans, rapeseed, sunflower, safflower, peanuts, cottonseed, corn, rice, and barley, as well as lecithin derived from egg yolks and their derivatives (hereinafter also referred to as lecithin derivatives).

[0090] Examples of lecithin derivatives include hydrogenated lecithin compounds and compounds formed by introducing polyethylene glycol, amino polysaccharides, etc., into the phospholipids of lecithin.

[0091] Of the above, from the viewpoint of acidification stability, hydrogenated lecithin is particularly preferred.

[0092] From the viewpoint of the long-term stability of the liposome composition for cosmetic use, the content of phosphatidylcholine relative to the total mass of lecithin (hereinafter also referred to as PC content) is preferably 80% by mass or more, more preferably 85% by mass or more, and even more preferably 90% by mass or more.

[0093] In addition, the PC content was determined by TLC-FID or HPLC of the phospholipid composition.

[0094] Commercially available lecithin products include COATSOME NC-21 (PC content of 90% or more by mass) manufactured by NOF CORPORATION and Nikkol (registered trademark) Lecinol S-10E (PC content of 75% to 85% or more by mass) manufactured by Nikko Chemicals Co., Ltd.

[0095] From the viewpoint of lipid bilayer formation and stability over time, the content of lecithin relative to the total mass of the cosmetic liposome composition is preferably 0.3% to 10% by mass, more preferably 0.4% to 5% by mass, and even more preferably 0.5% to 3% by mass.

[0096] (Cholesterol and phytosterols other than polyoxyethylene phytosterols)

[0097] In this invention, cholesterol includes cholesterol alcohol and cholesterol derivatives.

[0098] Examples of cholesterol include cholesterol, dihydrocholesterol, dehydrocholesterol, cholesterol oleate, cholesterol isostearate, cholesterol hydroxystearate, and polyoxyethylene cholesterol ether.

[0099] Furthermore, phytosterols other than polyoxyethylene phytosterols refer to phytosterols that do not contain polyoxyethylene.

[0100] Furthermore, from the viewpoint of the content of beauty ingredients, the cosmetic liposome composition of the present invention preferably contains at least cholesterol.

[0101] The content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol relative to the total mass of the cosmetic liposome composition is preferably 0.05% to 0.7% by mass, more preferably 0.1% to 0.5% by mass, and even more preferably 0.2% to 0.4% by mass.

[0102] By ensuring that the content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol is within the above-mentioned range, the proportion of liposomes with a monolayer structure in the cosmetic liposome composition can be increased, thereby further improving the content of cosmetic ingredients in the liposomes.

[0103] Furthermore, in this invention, "the content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol relative to the total mass of the cosmetic liposome composition" refers to the content of cholesterol when the cosmetic liposome composition contains only cholesterol, the content of cholesterol when the cosmetic liposome composition contains only phytosterols, and the sum of their contents when the cosmetic liposome composition contains both cholesterol and phytosterols.

[0104] (Internal and external water phases)

[0105] The cosmetic liposome composition of the present invention contains an internal aqueous phase and an external aqueous phase.

[0106] The inner aqueous phase contains at least water, ethanol, and cosmetic ingredients, while the outer aqueous phase contains at least water and ethanol. By including ethanol in both the inner and outer aqueous phases, the formation and long-term stability of liposomes can be improved.

[0107] (water)

[0108] Examples of water types include ion-exchanged water, pure water, purified water, and tap water. Among these, purified water is preferred for its suitability for cosmetics.

[0109] The water content relative to the total mass of the cosmetic liposome composition is not particularly limited, but is preferably 1% to 99% by mass, more preferably 10% to 95% by mass, and even more preferably 20% to 90% by mass.

[0110] Furthermore, in this invention, "the water content relative to the total mass of the cosmetic liposome composition" refers to the sum of the water content in the internal aqueous phase relative to the total mass of the cosmetic liposome composition and the water content in the external aqueous phase relative to the total mass of the cosmetic liposome composition.

[0111] (ethanol)

[0112] From the viewpoint of long-term stability, the content of ethanol relative to the total mass of the cosmetic liposome composition is preferably 0.2% to 30% by mass.

[0113] Furthermore, the content of ethanol relative to the total mass of the cosmetic liposome composition can be 3% by mass or more, 9% by mass or more, or 15% by mass or more.

[0114] Furthermore, the content of ethanol relative to the total mass of the cosmetic liposome composition may be less than 27% by mass, less than 25% by mass, less than 23% by mass, or less than 10% by mass.

[0115] Furthermore, in this invention, "the content of ethanol relative to the total mass of the cosmetic liposome composition" refers to the sum of the content of ethanol in the internal aqueous phase relative to the total mass of the cosmetic liposome composition and the content of ethanol in the external aqueous phase relative to the total mass of the cosmetic liposome composition.

[0116] In addition, ethanol includes anhydrous ethanol.

[0117] (beauty ingredients)

[0118] As cosmetic ingredients, examples include ascorbic acid, ascorbic acid stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylpentene ethyl ester, disodium adenosine triphosphate, disodium adenosine monophosphate, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin-β-glycyrrhetinic acid, albumin, inositol, erythritol, glucosamine hydrochloride, and pyridoxine hydrochloride. Alcohol, Hydroxyproline, Orotic Acid, Hydrolyzed Elastin, Caffeine Hydrate, Chondroitin Sulfate Sodium, Cyanocobalamin, Water-Soluble Elastin, Taurine, Sodium Palmitoyl Methyl Taurate, Sodium Myristoyl Methyl Taurate, Thiamine Hydrochloride, Theanine, Deoxyribonucleic Acid, Ascorbyl Palmitate, Panthenol, Sodium Pantothenate, Calcium Pantothenate, Pyridoxine, Phytic Acid, Placental Extract, Flavin Adenine Dinucleotide Disodium Dihydrate, Anhydrous Caffeine, Riboflavin Riboflavin butyrate, riboflavin phosphate, resorcinol, acetylpropionic acid, glycine, arginine, lysine solution, lauroyl lysine, aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, retinyl palmitate, alginic acid, γ-undecyl lactone, perilla oil, estradiol, estrone, ergocalciferol, β-carotene, glucosamine, cholecalciferol, tocopherol acetate, retinyl acetate, shikonin, ascorbate dipalmitate, pyridoxine dipalmitate, natural vitamin E, α-tocopherol, horse oil, γ-nonalactone, bisabolol, vitamin A oil, cypress thiol, fumaric acid, powdered vitamin A, linoleic acid tocopherol, δ-tocopherol, isoleucine, phenylalanine, valine, leucine, ascorbate tetra-2-hexyldecanoate, etc. In addition, caffeine includes anhydrous caffeine.

[0119] From a solubility perspective, the cosmetic ingredients contained in the inner aqueous phase are preferably water-soluble cosmetic ingredients. Among the above, preferred ingredients include ascorbic acid, ascorbic acid stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylacetonate, disodium adenosine triphosphate, disodium adenosine monophosphate, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin β-glycyrrhetinic acid, albumin, inositol, erythritol, glucosamine hydrochloride, pyridoxine hydrochloride, hydroxyproline, orotic acid, hydrolyzed elastin, caffeine hydrate, sodium chondroitin sulfate, cyanocobalamin, and water-soluble ingredients. Elastin, taurine, sodium palmitoyl methyl taurate, sodium myristoyl methyl taurate, thiamine hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, panthenol, sodium pantothenate, calcium pantothenate, pyridoxine, phytic acid, placental extract, disodium flavin adenine dinucleotide dihydrate, anhydrous caffeine, riboflavin, riboflavin butyrate, riboflavin phosphate, resorcinol, acetylpropionic acid, glycine, arginine, lysine solution, lauroyl lysine, aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, etc.

[0120] Furthermore, from the perspective of anti-aging, anti-wrinkle, anti-freckle, and anti-sagging, the aqueous phase preferably contains at least one selected from caffeine, pyridoxine hydrochloride, and nicotinamide.

[0121] In addition, cosmetic ingredients can also be contained in the external aqueous phase.

[0122] The content of the cosmetic ingredient relative to the total mass of the cosmetic liposome composition is preferably 0.3% to 5% by mass, more preferably 0.5% to 3% by mass.

[0123] The internal and external aqueous phases may also contain physiological saline, sugars, pH adjusters, preservatives, and other components. Examples of sugars include glucose, fructose, lactose, sucrose, trehalose, lactulose, and maltitol. Examples of pH adjusters include sodium hydrogen phosphate, sodium dihydrogen phosphate, anhydrous disodium hydrogen phosphate, sodium hydroxide (caustic soda), citric acid, acetic acid, and triethanolamine. Examples of preservatives include methylparaben.

[0124] The pH of the internal and external aqueous phases is not particularly limited, but is preferably 5 to 9, more preferably 7 to 8. The pH of the internal and external aqueous phases can be adjusted by using the pH adjuster described above.

[0125] The following describes a method for manufacturing the cosmetic liposome composition of the present invention, but is not limited thereto.

[0126] Liposome compositions for cosmetic use can be manufactured by mixing an oil phase composition and an aqueous phase composition, stirring them to emulsify them, and forming liposomes.

[0127] A cosmetic liposome composition containing liposomes, an inner aqueous phase, and an outer aqueous phase is manufactured by mixing, stirring, and emulsifying the oil phase composition and the aqueous phase composition into an O / W (oil-in-water) type.

[0128] As a stirring method, ultrasound or mechanical shearing force is used. Furthermore, to achieve uniform particle size, extrusion processing or microfluidic processing via a filter with a constant pore size can be performed. Through extrusion processing or similar methods, multi-capsule liposomes containing multiple liposomes can be separated into multiple liposomes.

[0129] The liquid temperature of the mixture can be adjusted appropriately, but it is preferred to set it to a temperature higher than the phase transition temperature of the lecithin contained in the mixture, for example, preferably 35°C to 70°C.

[0130] In the manufacture of liposome compositions for cosmetic use, organic solvents and water can also be evaporated from an aqueous solution containing liposomes.

[0131] The aforementioned evaporation includes either intentionally causing part or all of the organic solvent and water to evaporate, or causing part or all of the organic solvent and water to evaporate naturally during stirring and emulsification.

[0132] When the organic solvent and water are intentionally evaporated, there is no particular limitation on the evaporation method. Examples include heating the organic solvent and water, allowing the cosmetic liposome composition to stand, stirring the cosmetic liposome composition, and performing vacuum degassing.

[0133] There are no particular limitations on the method of containing cosmetic ingredients in liposomes; it can be done by using an aqueous composition in which the cosmetic ingredients are dissolved during emulsification.

[0134] The obtained cosmetic liposome composition can be subjected to dialysis, filtration, or extrusion processes to ensure a uniform average particle size of the contained liposomes. Extrusion processing involves passing the cosmetic liposome composition through a fine-pore filter, thereby applying physical shear force to micronize it. When passing the cosmetic liposome composition through the filter, rapid micronization can be achieved by maintaining both the cosmetic liposome composition and the filter at a temperature above the phase transition temperature of lecithin.

[0135] (use)

[0136] In one embodiment, the cosmetic liposome composition of the present invention is a cosmetic product. The form of the cosmetic product is not particularly limited; it can be a liquid, a jelly, a gel, a cream, a solid such as a stick, or a semi-solid form. Examples of cosmetic products include skincare cosmetics (lotions, lotions, serums, sunscreens, etc.), body cosmetics (body lotions, etc.), scalp cosmetics, and face masks, but are not limited to these.

[0137] All documents, patent applications and technical standards described in this specification are referenced in this specification to the same extent that each document, patent application and technical standard is specifically and separately described and referenced by reference.

[0138] Example

[0139] The above embodiments will be described in more detail below through examples, but the above embodiments are not limited to these examples.

[0140] <Example 1-1>

[0141] (Preparation of oil phase composition)

[0142] Hydrogenated soybean lecithin (hydrogenated lecithin, PC content ≥ 90% by mass), polyoxyethylene phytosterol A (average addition molar number of oxyethylene: 20), cholesterol, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition A.

[0143] (Preparation of aqueous phase composition)

[0144] Anhydrous caffeine (a beauty ingredient), niacinamide, anhydrous disodium hydrogen phosphate, sodium dihydrogen phosphate, and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition A.

[0145] (Manufacturing of liposome compositions for cosmetic use)

[0146] The aqueous phase composition A obtained above was kept at 60°C and stirred using a homogenizer (3400 rpm). Oil phase composition A was then added to the aqueous phase composition A.

[0147] After addition, stirring was continued at 3400 rpm for 45 minutes. After stirring, the mixture was cooled to below 35°C and then filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

[0148] In addition, the content of each component in the cosmetic liposome composition is shown in Table 1.

[0149] In the cosmetic liposome composition obtained in Examples 1-1, the ratio of the content of polyoxyethylene phytosterol to the content of ethanol (content of polyoxyethylene phytosterol / content of ethanol) is 0.002 on a mass basis.

[0150] Furthermore, in the cosmetic liposome composition obtained in Examples 1-1, the ratio of the content of polyoxyethylene phytosterols to the content of cholesterol (content of polyoxyethylene phytosterols / content of cholesterol) is 0.2 on a mass basis.

[0151] In addition, in Table 1, “P / E” represents the content of polyoxyethylene phytosterols / ethanol, and “P / C” represents the content of polyoxyethylene phytosterols / cholesterol.

[0152] For subsequent embodiments, the content of each component, P / E and P / C are also shown in Tables 1 to 3.

[0153] <Examples 1-2 to Examples 1-4 and Comparative Examples 1-1 to 1-5>

[0154] The composition of the cosmetic liposome composition was changed as shown in Table 1, except that the cosmetic liposome composition was manufactured in the same manner as in Examples 1-1.

[0155] In addition, liposomes were not formed in Comparative Example 1-1, so the subsequent evaluation results are marked as "-" in Table 1.

[0156] <Examples 1-5>

[0157] (Preparation of oil phase composition)

[0158] Hydrogenated soybean lecithin (containing more than 90% by mass of hydrogenated lecithin and PC), polyoxyethylene phytosterol A (average molar addition of oxyethylene: 20), cholesterol, oleic acid, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition B.

[0159] (Preparation of aqueous phase composition)

[0160] Pyridoxine hydrochloride (a cosmetic ingredient), nicotinamide (a cosmetic ingredient), anhydrous disodium hydrogen phosphate, sodium hydroxide solution (caustic soda), and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition B.

[0161] (Preparation of liposome compositions)

[0162] The aqueous phase composition B obtained above was kept at 60°C and stirred using a homogenizer (3000 rpm). Then, the oil phase composition B was added to the aqueous phase composition B.

[0163] After addition, continue stirring at 3000 rpm for 45 minutes. After stirring, cool to below 35°C, filter through a 230-mesh filter cloth, and then evaporate the ethanol using an evaporator to obtain a liposome composition for cosmetic use.

[0164] In addition, the content of each component in Table 1 is the content in the cosmetic liposome composition after ethanol evaporation.

[0165] <Examples 1-6 to Examples 1-7>

[0166] The composition of the cosmetic liposome composition was changed as shown in Table 1, except that the cosmetic liposome composition was manufactured in the same manner as in Examples 1-5.

[0167] <Example 2-1 to Example 2-3>

[0168] (Preparation of oil phase composition)

[0169] Hydrogenated soybean lecithin (hydrogenated lecithin, PC content ≥ 90% by mass), polyoxyethylene phytosterol B (average addition molar number of oxyethylene: 30), cholesterol, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition C1.

[0170] (Preparation of aqueous phase composition)

[0171] Anhydrous caffeine (cosmetic ingredient), niacinamide (cosmetic ingredient), anhydrous disodium hydrogen phosphate, sodium dihydrogen phosphate, and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition C.

[0172] (Manufacturing of liposome compositions for cosmetic use)

[0173] The aqueous phase composition C obtained above was kept at 60°C and stirred using a homogenizer (3400 rpm) before the oil phase composition C1 was added to the aqueous phase composition C.

[0174] After addition, stirring was continued at 3400 rpm for 45 minutes. After stirring, the mixture was cooled to below 35°C and then filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

[0175] <Example 2-4>

[0176] (Preparation of oil phase composition)

[0177] Hydrogenated soybean lecithin (hydrogenated lecithin, PC content ≥ 90% by mass), polyoxyethylene phytosterol C (average addition molar number of oxyethylene: 10), cholesterol, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition C2.

[0178] (Preparation of aqueous phase composition)

[0179] Anhydrous caffeine (cosmetic ingredient), niacinamide (cosmetic ingredient), anhydrous disodium hydrogen phosphate, sodium dihydrogen phosphate, and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition C.

[0180] (Manufacturing of liposome compositions for cosmetic use)

[0181] The aqueous phase composition C obtained above was kept at 60°C and stirred using a homogenizer (3400 rpm) before the oil phase composition C2 was added to the aqueous phase composition C.

[0182] After addition, stirring was continued at 3400 rpm for 45 minutes. After stirring, the mixture was cooled to below 35°C and then filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

[0183] <Examples 2-5>

[0184] (Preparation of oil phase composition)

[0185] Hydrogenated soybean lecithin (hydrogenated lecithin, PC content ≥ 90% by mass), polyoxyethylene phytosterol D (average addition molar number of oxyethylene: 5), cholesterol, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition C3.

[0186] (Preparation of aqueous phase composition)

[0187] Anhydrous caffeine (cosmetic ingredient), niacinamide (cosmetic ingredient), anhydrous disodium hydrogen phosphate, sodium dihydrogen phosphate, and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition C.

[0188] (Manufacturing of liposome compositions for cosmetic use)

[0189] The aqueous phase composition C obtained above was kept at 60°C and stirred using a homogenizer (3400 rpm) before the oil phase composition C3 was added to the aqueous phase composition C.

[0190] After addition, stirring was continued at 3400 rpm for 45 minutes. After stirring, the mixture was cooled to below 35°C and then filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

[0191] <Example 3-1>

[0192] (Preparation of oil phase composition)

[0193] Hydrogenated soybean lecithin (hydrogenated lecithin, PC content ≥ 90% by mass), polyoxyethylene phytosterol A (average addition molar number of oxyethylene: 20), cholesterol, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition D.

[0194] (Preparation of aqueous phase composition)

[0195] Pyridoxine hydrochloride (cosmetic ingredient), anhydrous caffeine (cosmetic ingredient), nicotinamide (cosmetic ingredient), anhydrous disodium hydrogen phosphate, sodium dihydrogen phosphate, and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition D-1.

[0196] Furthermore, methylparaben and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition D-2.

[0197] (Manufacturing of liposome compositions for cosmetic use)

[0198] The aqueous phase composition D-1 obtained above was kept at 60°C and stirred using a homogenizer (3400 rpm) before the oil phase composition D was added to the aqueous phase composition D-1.

[0199] After addition, stirring was continued at 3400 rpm for 45 minutes. Following stirring, aqueous phase composition D-2 was added, and the mixture was stirred uniformly for 5 minutes, then cooled to below 35°C. After cooling, the mixture was filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

[0200] <Example 3-2>

[0201] (Preparation of oil phase composition)

[0202] Hydrogenated soybean lecithin (containing more than 90% by mass of hydrogenated lecithin and PC), polyoxyethylene phytosterol A (average addition molar number of oxyethylene: 20), cholesterol, oleic acid, anhydrous ethanol and ascorbic acid tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes to obtain oil phase composition E.

[0203] (Preparation of aqueous phase composition)

[0204] Pyridoxine hydrochloride (a cosmetic ingredient), nicotinamide (a cosmetic ingredient), anhydrous disodium hydrogen phosphate, sodium hydroxide solution (caustic soda), and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous composition E-1.

[0205] Furthermore, methylparaben and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain an aqueous composition E-2.

[0206] (Manufacturing of liposome compositions for cosmetic use)

[0207] The aqueous phase composition E-1 obtained above was kept at 60°C and stirred using a homogenizer (3000 rpm) before the oil phase composition E was added to the aqueous phase composition E-1.

[0208] After addition, stirring was continued at 3000 rpm for 45 minutes. Following stirring, aqueous phase composition E-2 was added, and the mixture was stirred uniformly for 5 minutes, then cooled to below 35°C. After cooling, the mixture was filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

[0209] <<Inclusion Rate Evaluation - 1 (Proportion of Liposomes with Monolayer Structure)>>

[0210] The liposomes contained in the cosmetic liposome compositions manufactured in the examples and comparative examples were obtained at 50,000x magnification using a transmission electron microscope in a frozen state.

[0211] At any three locations in the TEM image, the average ratio of the total number of liposomes within a circle with a radius of 2 μm and the total number of liposomes with a monolayer structure was determined according to the following evaluation criteria. The evaluation results are shown in Tables 1 to 3.

[0212] Furthermore, TEM images of Examples 1-2, 3-1, 3-2, Comparative Examples 1-2, and 1-5 are shown below. Figures 1-5 In addition, regarding the total number of liposomes and the total number of liposomes with a monolayer structure, Figures 1-5 The circle shown represents any one point from which the total number of liposomes and the total number of liposomes with a monolayer structure are counted.

[0213] (Evaluation Criteria)

[0214] A: The proportion of liposomes with a monolayer structure is over 70%.

[0215] B: The proportion of liposomes with a monolayer structure is more than 50% and less than 70%.

[0216] C: The proportion of liposomes with a monolayer structure is more than 30% and less than 50%.

[0217] D: The proportion of liposomes with a monolayer structure is less than 30%.

[0218] <<Inclusion Evaluation - 2 (Average Particle Size of Liposomes)>>

[0219] The cosmetic liposome compositions manufactured in the examples and comparative examples were diluted 10 times their mass with pure water, and the volume average particle size of the contained liposomes was determined by dynamic light scattering using a thick-film particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.). The volume average particle size determination was performed within 20 to 24 hours after manufacturing the cosmetic liposome compositions. The ambient temperature for the determination was set to 25°C. The results are shown in Tables 1 to 3.

[0220] <<Time-bound Stability Evaluation>>

[0221] The cosmetic liposome compositions prepared in Examples 1-5 to 1-7, Examples 2-1 to 2-5, Examples 3-1 to 3-2 and Comparative Examples 1-4 were left to stand at 50°C for 1 week.

[0222] The cosmetic liposome composition, after being left to stand, was diluted with pure water to 10 times its mass, and the volume average particle size of the liposomes contained in the cosmetic liposome composition was determined by the above method.

[0223] The volume-average particle size of the liposomes in the liposome composition after standing was calculated relative to the volume-average particle size of the liposomes in the freshly manufactured cosmetic liposome composition (volume-average particle size of liposomes in the liposome composition after standing / volume-average particle size of liposomes in the freshly manufactured cosmetic liposome composition). The long-term stability of the cosmetic liposome composition was evaluated according to the following evaluation criteria. The evaluation results are shown in Tables 1 to 3.

[0224] In addition, in Comparative Examples 1-2 and 1-3, since the proportion of liposomes with monolayer structure was small and the content of cosmetic ingredients was insufficient, no time-stability evaluation was performed. In Table 1, the evaluation results are marked as "-".

[0225] Furthermore, in Comparative Examples 1-5, the internal rate evaluation was insufficient, therefore no long-term stability evaluation was performed. In Table 1, the evaluation result is marked as "-".

[0226] (Evaluation Criteria)

[0227] A: The ratio of volume average particle size is less than 1.1.

[0228] B: The ratio of volume average particle size is 1.1 or higher and less than 1.4.

[0229] C: The ratio of volume average particle size is 1.4 or higher.

[0230]

[0231] [Table 2]

[0232]

[0233] [Table 3]

[0234]

[0235] As shown in Table 1, the liposome compositions obtained in Examples 1-1 to 1-7, compared with the liposome compositions obtained in Comparative Examples 1-2 to 1-3 that do not contain at least one of polyoxyethylene phytosterol and ethanol, have a higher proportion of liposomes with a monolayer structure and a better content of cosmetic ingredients.

[0236] Furthermore, as shown in Table 1, the liposome compositions obtained in Examples 1-1 to 1-7, compared with the liposome compositions obtained in Comparative Examples 1-5 that do not contain cholesterol and at least one of the phytosterols other than polyoxyethylene phytosterol, but contain liposomes with an average particle size of less than 100 nm, have a higher proportion of liposomes with a monolayer structure and a superior content of cosmetic ingredients.

[0237] Furthermore, it can be seen that the liposome compositions obtained in Examples 1-1 to 1-7 contain liposomes with a large average particle size and excellent content of cosmetic ingredients.

[0238] In addition, in Comparative Examples 1-2 to 1-3, the content of the cosmetic ingredients was the same as in Examples 1-1 to 1-7, but the proportion of liposomes with a monolayer structure in the obtained liposome compositions was small. Therefore, most of the cosmetic ingredients were contained in the outer aqueous phase rather than the inner aqueous phase.

[0239] Compared with the liposome compositions obtained in Comparative Examples 1-4 that do not contain polyoxyethylene phytosterols but contain liposomes with an average particle size of more than 350 nm, the liposome compositions obtained in Examples 1-1 to 1-7 have excellent stability over time.

[0240] In addition, since Comparative Example 1-1 does not contain ethanol, it is presumed that the liposome formation is low.

[0241] As can be seen from the results in Tables 2 and 3, the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 have a large proportion of liposomes with a monolayer structure and excellent content of cosmetic ingredients.

[0242] Furthermore, it can be seen that the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 contain liposomes with large average particle size and excellent content of cosmetic ingredients.

[0243] Furthermore, it is known that the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 exhibit excellent stability over time.

[0244] Depend on Figures 1-5 It can be seen that the liposome composition obtained in the examples has a higher proportion of liposomes with a monolayer structure and a superior content of cosmetic ingredients compared to the liposome composition obtained in the comparative examples. Furthermore, from Figure 5 It is known that it contains a large number of disk particles, rather than liposomes.

[0245] Hereinafter, examples of formulations of the cosmetic liposome composition of the present invention are disclosed when it is used as a cosmetic.

[0246] <Example 4-1: Cream>

[0247] A cream having the following composition (total amount 100% by mass) was prepared by conventional methods.

[0248] (composition)

[0249]

[0250]

[0251] <Example 4-2: Cream>

[0252] A cream having the following composition (total amount 100% by mass) was prepared by conventional methods.

[0253] (composition)

[0254]

[0255]

[0256] It is evident that the creams obtained in Examples 4-1 and 4-2 have good properties in terms of feel and fragrance, and the cosmetic liposome composition of the present invention can be appropriately used to prepare creams.

[0257] <Example 5-1: Toner>

[0258] A toner with the following composition (total amount 100% by mass) was prepared using conventional methods.

[0259] (composition)

[0260]

[0261]

[0262] (Average molar addition of ethylene oxide: 60)

[0263]

[0264] (Manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-20)

[0265]

[0266] <Example 5-2: Toner>

[0267] A toner with the following composition (total amount 100% by mass) was prepared using conventional methods.

[0268] (composition)

[0269]

[0270]

[0271] (Average molar addition of ethylene oxide: 60)

[0272]

[0273] (Manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-20)

[0274]

[0275] It is evident that the toners obtained in Examples 5-1 and 5-2 have excellent properties in terms of user experience and fragrance, and the cosmetic liposome composition of the present invention can be appropriately used to prepare toners.

[0276] The lycopene-containing emulsified compositions used in Examples 5-1 and 5-2 were prepared by the following method.

[0277] <Preparation of emulsified compositions containing lycopene>

[0278] (Preparation of oil phase composition a)

[0279] Weigh the following components in a container and mix them on a hot plate at 150°C while stirring for 5 minutes to obtain oil phase composition a.

[0280] (composition)

[0281]

[0282] (Manufactured by Riken Vitamin Co., Ltd., Riken E Oil800)

[0283] (Preparation of aqueous composition a)

[0284] The components of the following composition were weighed in a container and mixed by stirring and heating in a constant temperature bath at 70°C to obtain aqueous composition a.

[0285] (composition)

[0286] · 10 parts by weight of oleic acid decaglyceride

[0287] (Manufactured by Nikko Chemicals Co., Ltd., Decaglin 1-O)

[0288] 45 parts by weight of glycerin

[0289] · 30 parts by weight of purified water

[0290] The obtained aqueous phase composition a was added to the oil phase composition a and stirred. The mixture was then dispersed for a specified time using an ultrasonic homogenizer to obtain a coarse dispersion.

[0291] Then, the obtained coarse dispersion was emulsified at 200 MPa using an ultra-high pressure emulsification device (manufactured by SUGINO MACHINE LIMITED, ULTIMAIZER) to prepare an emulsified composition containing lycopene (lycopene content: 0.17% by mass).

[0292] The obtained lycopene-containing emulsion composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was determined using an FPAR-1000 particle size analyzer (manufactured by Otsuka Electronics Co., Ltd.), which showed a result of 52 nm.

[0293] <Example 6-1: Jelly-like Beauty Essence>

[0294] A jelly-like beauty liquid with the following composition (total amount 100% by mass) was prepared by conventional methods.

[0295] (composition)

[0296] • 2.0% by mass of the liposome composition of Example 3-1

[0297] ·0.1% by weight of Haematococcus pluvialis extract

[0298] • 0.2% by weight of an emulsion composition containing astaxanthin

[0299] (Krill extract, the composition prepared by the method described below)

[0300]

[0301] (Manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-20)

[0302] · 0.2% by weight of polyoxyethylene hydrogenated castor oil

[0303] (Average molar addition of ethylene oxide: 60)

[0304]

[0305] <Example 6-2: Jelly-like Beauty Essence>

[0306] A jelly-like beauty liquid with the following composition (total amount 100% by mass) was prepared by conventional methods.

[0307] (composition)

[0308] • 2.0% by mass of the liposome composition of Example 3-2

[0309] ·0.1% by weight of Haematococcus pluvialis extract

[0310] • 0.2% by weight of an emulsion composition containing astaxanthin

[0311] (Krill extract, the composition prepared by the method described below)

[0312]

[0313] (Manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-20)

[0314] · 0.2% by weight of polyoxyethylene hydrogenated castor oil

[0315] (Average molar addition of ethylene oxide: 60)

[0316]

[0317]

[0318] It is evident that the jelly-like beauty liquids obtained in Examples 6-1 and 6-2 possess excellent properties in terms of user experience and fragrance, and the cosmetic liposome composition of the present invention can be appropriately used to prepare jelly-like beauty liquids.

[0319] The astaxanthin-containing emulsified compositions used in Examples 6-1 and 6-2 were prepared by the following method.

[0320] <Preparation of emulsified compositions containing astaxanthin>

[0321] (Preparation of aqueous composition b)

[0322] The following components were heated at 70°C and dissolved for 1 hour to obtain aqueous composition b.

[0323] (composition)

[0324]

[0325] (Preparation of oil phase composition b)

[0326] The following components were heated at 70°C and dissolved for 1 hour to obtain oil phase composition b.

[0327] (composition)

[0328] Krill extract 15.0g

[0329] Mixed tocopherols 32.0g

[0330] (Manufactured by Riken Vitamin Co., Ltd., Riken E Oil800)

[0331] Medium-chain triglycerides 93.0g

[0332] (Manufactured by Kao Corporation, Coconard (registered trademark) MT)

[0333] Lecithin 10.0g

[0334] (Manufactured by Riken Vitamin Co., Ltd., LECION (registered trademark) P, derived from soybeans)

[0335] The aqueous phase composition B obtained above was kept at 70°C and stirred (10,000 rpm) using a homogenizer (SMTCo., Ltd., HP93). Then, the oil phase composition B was added to the aqueous phase composition B to obtain a pre-emulsion.

[0336] The obtained pre-emulsion was cooled to approximately 40°C and subjected to high-pressure emulsification at 200 MPa using a ULTIMAIZER HJP-25005 (manufactured by SUGINOMACHINE LIMITED). The emulsion was then filtered through a microfilter with an average pore size of 1 μm to prepare an astaxanthin-containing emulsion composition (astaxanthin content: 0.3% by mass).

[0337] The obtained astaxanthin emulsion composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was determined using an FPAR-1000 particle size analyzer (manufactured by Otsuka Electronics Co., Ltd.), which showed a result of 58 nm.

[0338] <Example 7-1: Sunscreen Agent>

[0339] A sunscreen agent with the following composition (total amount 100% by mass) was prepared by conventional methods.

[0340] (composition)

[0341]

[0342]

[0343] (Manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-20)

[0344] · 0.2% by weight of polyoxyethylene hydrogenated castor oil

[0345] (Average molar addition of ethylene oxide: 60)

[0346]

[0347] <Example 7-2: Sunscreen Agent>

[0348] A sunscreen agent with the following composition (total amount 100% by mass) was prepared by conventional methods.

[0349] (composition)

[0350]

[0351]

[0352] (Manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-20)

[0353] · 0.2% by weight of polyoxyethylene hydrogenated castor oil

[0354] (Average molar addition of ethylene oxide: 60)

[0355]

[0356] It is evident that the sunscreens obtained in Examples 7-1 and 7-2 have good properties in terms of user experience and fragrance, and the cosmetic liposome composition of the present invention can be appropriately used to prepare sunscreens.

Claims

1. A liposome composition for cosmetic use, comprising: The liposomes contain polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and phytosterols other than the polyoxyethylene phytosterol, and the average particle size of the liposomes is 100 nm to 350 nm. An internal aqueous phase containing water, ethanol, and cosmetic ingredients, and contained within the liposomes; and An external aqueous phase containing water and ethanol is used to disperse the liposomes. The cosmetic ingredient is a water-soluble cosmetic ingredient. in, The average molar number of oxyethylene vinyl groups added to the polyoxyethylene phytosterol is 5 or more. Relative to the total mass of the cosmetic liposome composition, the content of the polyoxyethylene phytosterol is 0.05% to 0.70% by mass, the content of the lecithin is 0.3% to 10% by mass, the content of at least one selected from cholesterol and phytosterols other than the polyoxyethylene phytosterol is 0.05% to 0.7% by mass, the sum of the content of ethanol in the inner aqueous phase and the content of ethanol in the outer aqueous phase is 0.2% to 30% by mass, and the content of the cosmetic ingredient is 0.3% to 5% by mass.

2. The cosmetic liposome composition according to claim 1, wherein, The average molar number of oxyethylene vinyl groups added to the polyoxyethylene phytosterol is 10 or more.

3. The cosmetic liposome composition according to claim 1, wherein, The polyoxyethylene phytosterol content relative to the cosmetic liposome composition is 0.1% to 0.70% by mass.

4. The cosmetic liposome composition according to claim 1, wherein, The sum of the content of ethanol in the inner aqueous phase and the content of ethanol in the outer aqueous phase relative to the total mass of the cosmetic liposome composition is 0.2% to 25% by mass.

5. The cosmetic liposome composition according to claim 1, wherein, The content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol is 0.1% to 0.5% by mass relative to the total mass of the cosmetic liposome composition.

6. The cosmetic liposome composition according to claim 1, wherein, The cosmetic ingredient is selected from at least one of caffeine, pyridoxine hydrochloride, and niacinamide.

7. The cosmetic liposome composition according to any one of claims 1 to 6, wherein, The total number of liposomes with a monolayer structure accounts for more than 70% of the total number of liposomes contained in the cosmetic liposome composition.

8. A cosmetic product comprising a liposome composition for cosmetic use according to any one of claims 1 to 7.

Images