一种提高核酸聚合测序质量的方法、反应体系和试剂盒
By adding blocking modified nucleotides to the nucleic acid polymerization sequencing reaction solution, the problem of fluorescent modification affecting the recognition and synthesis efficiency of DNA polymerase was solved, resulting in higher sequencing quality and a lower error rate.
CN115141880BActive Publication Date: 2026-07-17MGI TECH CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- MGI TECH CO LTD
- Filing Date
- 2017-01-10
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
In existing nucleic acid polymerization sequencing methods, the use of fluorescently modified nucleotides leads to low DNA polymerase recognition and synthesis efficiency, limiting read length and accuracy.
Method used
Adding a second type of nucleotide with only blocking modification and no fluorescent modification to the polymerization sequencing reaction solution can supplement the incomplete part of the reaction, improve synthesis efficiency and reduce sequencing error rate.
Benefits of technology
It improved sequencing quality, reduced sequencing error rate and signal interference, and enhanced sequencing accuracy and efficiency.
✦ Generated by Eureka AI based on patent content.
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Figure CN115141880B_ABST
Abstract
本申请公开了一种提高核酸聚合测序质量的方法、反应体系和试剂盒。本申请的提高核酸聚合测序质量的方法，包括在聚合测序的反应液中加入第二类核苷酸，第二类核苷酸具有在3’糖羟基上的阻断修饰但没有荧光修饰。本申请的提高核酸聚合测序质量的方法，在其反应液中添加仅仅具有阻断修饰的dNTPs，由于这类dNTPs没有荧光修饰，更利于DNA聚合酶识别和合成，能够补充反应不完全的部分，提高合成反应的效率，降低反应不充分的风险；与此同时，由于没有荧光修饰，降低了因荧光切除不干净，或者切除的荧光洗脱不干净导致的信号干扰，提高了切除效率，降低了测序错误率。
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