Natural plant preparation for relieving stress in animals and method for its preparation
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-09-02
- Publication Date
- 2026-08-11
AI Technical Summary
[0004]本发明的目的在于提供一种缓解动物应激的天然植物制剂及其制备方法，其解决了现有缓解动物应激的药物制剂所存在的价格昂贵、存在毒副作用的缺陷
[0024]本发明的有益效果在于：该制剂中柑橘富含维生素C和萜类化合物，能起到中枢神经镇静化的作用；青橄榄富含营养元素钙，可避免内质网代谢失衡、功能紊乱；松果富含N-乙酰基-5-甲氧基色胺，有助于调节精神状态和改善睡眠质量；火桑桑叶中的γ-氨基丁酸作为抑制性神经递质，可起到镇静、催眠、抗惊厥的作用；胡杨碱则有利于维持动物机体的电解质和酸碱平衡；而尾穗苋提取物和刺沙蓬提取物的搭配使用，则起到了舒缓降压、镇静作用，且可明显减少动物因应激反应而引起的下丘脑内促肾上腺皮质激素的分泌释放，避免下丘脑-垂体-肾上腺轴的过度激活，从而缓解应激反应以及所带来的动物机体功能损害。
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Abstract
Description
Technical Field
[0001] This invention relates to the field of veterinary drug technology, specifically to a natural plant preparation for relieving animal stress and its preparation method. Background Technology
[0002] The stress response in animals is an adaptive response of the animal body to changes in its internal and external environment. Stress responses are triggered by a variety of factors, including climatic factors, environmental fright, husbandry and management factors, nutritional factors, group feeding stress, density stress, transportation stress, weaning stress, pathogenic factors or diseases, genetic factors, and reproductive factors. These stressors can harm the animal's body, manifesting as lethargy, loss of appetite, extreme excitement, restlessness, reduced feed intake, weakened metabolism, decreased feed conversion rate, decreased production performance, weakened immunity, and allergies.
[0003] To alleviate stress in animals, various types of drug formulations have emerged on the market. However, these drug formulations generally contain chemical components or use components needed by the body as direct raw materials, resulting in high prices and varying degrees of toxic side effects that affect other physiological functions of animals. Summary of the Invention
[0004] The purpose of this invention is to provide a natural plant preparation for relieving animal stress and its preparation method, which solves the defects of existing drug preparations for relieving animal stress, such as high price and toxic side effects.
[0005] The present invention achieves the above objectives through the following technical solutions:
[0006] A natural plant-based preparation for relieving animal stress, comprising, by weight, the following raw materials:
[0007] Citrus fruits 0.1-20 parts;
[0008] 0.1-10 parts green olives;
[0009] 0.5-10 parts pine cones;
[0010] 0.05-5 parts of mulberry leaves;
[0011] Populus alkaloids 0.05-5 parts;
[0012] Plant extracts 0.01-3 parts;
[0013] The plant extract is composed of a mixture of Amaranthus caudatus extract and Amaranthus spinosa extract in a weight ratio of 2:1-4.
[0014] A further improvement is that the plant extract is a mixture of Amaranthus caudatus extract and Amaranthus spinosus extract in a 1:1 weight ratio.
[0015] A further improvement is that the raw materials for preparation also include an adjuvant, which accounts for 1-3% of the total weight of the preparation, and the adjuvant is linoleic acid, oleic acid or a mixture thereof.
[0016] This invention also provides a method for preparing the aforementioned natural plant preparation for alleviating animal stress, comprising the following steps:
[0017] Step 1: Prepare extracts of Amaranthus caudatus and Amaranthus spinosa separately and mix them to obtain plant extracts for later use;
[0018] Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use.
[0019] Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
[0020] A further improvement is that the preparation method of the amaranth extract is as follows: crush the amaranth seeds, add 5-10 times the weight of water, sonicate for 5-20 minutes, filter to obtain the residue, add 5-20 times the weight of 75-90% ethanol solution to the residue, reflux for 1-3 hours, filter to obtain the filtrate and residue, extract the residue again in the same way, combine the two filtrates, concentrate, dry and crush to obtain the amaranth extract.
[0021] A further improvement is that the ultrasonic processing power is 150-300W and the frequency is 30-50kHz.
[0022] A further improvement is that the preparation method of the *Symplocos pubescens* extract is as follows: *Symplocos pubescens* stems are crushed and then 3-5 times their weight of 1-4% lactic acid aqueous solution is added. The pH value is adjusted to 4.5-6, the temperature is raised to 45-50℃, and 0.5-3% of cellulase and 0.5-2% of pectinase by weight of *Symplocos pubescens* are added. The mixture is stirred evenly and enzymatically hydrolyzed for 40-60 minutes. During the enzymatic hydrolysis, an electric field is applied for 5-10 minutes to obtain the hydrolysate. The hydrolysate is filtered to obtain the filtrate, which is then freeze-dried and crushed to obtain the *Symplocos pubescens* extract.
[0023] A further improvement is that the electric field strength of the electric field treatment is 30-40 kV / cm, the pulse time is 400-600 μs, and the pulse frequency is 150-250 Hz.
[0024] The beneficial effects of this invention are as follows: citrus fruits in this preparation are rich in vitamin C and terpenoids, which can have a sedative effect on the central nervous system; green olives are rich in the nutrient element calcium, which can prevent endoplasmic reticulum metabolic imbalance and functional disorders; pine cones are rich in N-acetyl-5-methoxytryptamine, which helps regulate mental state and improve sleep quality; γ-aminobutyric acid in mulberry leaves, as an inhibitory neurotransmitter, can have sedative, hypnotic, and anticonvulsant effects; poplar alkaloids are beneficial for maintaining the electrolyte and acid-base balance of the animal body; and the combined use of extracts from amaranth and sedge has a soothing and sedative effect, and can significantly reduce the secretion and release of adrenocorticotropic hormone in the hypothalamus caused by stress response in animals, avoiding excessive activation of the hypothalamus-pituitary-adrenal axis, thereby alleviating stress response and the resulting damage to animal bodily functions.
[0025] This preparation is made entirely of natural plant ingredients, contains no chemical additives, has no toxic side effects under normal administration, and has a wide range of raw material sources and low prices. It can effectively alleviate stress responses in many types of animals and is suitable for widespread application. Attached Figure Description
[0026] Figure 1 This is a graph showing the results of adrenocorticotropic hormone (ACTH) level measurement. Detailed Implementation
[0027] The present application will be further described in detail below with reference to the embodiments. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.
[0028] Example 1
[0029] A natural plant preparation for relieving animal stress, comprising, by weight, the following raw materials: 0.1 parts citrus; 0.1 parts green olive; 10 parts pine cone; 5 parts fire mulberry leaves; 5 parts poplar alkaloid; and 0.01 parts plant extract; wherein the plant extract is a mixture of amaranth extract and sedge extract in a weight ratio of 2:1.
[0030] The method for preparing the natural plant preparation for alleviating animal stress includes the following steps:
[0031] Step 1: Prepare extracts of Amaranthus caudatus and Amaranthus spinosa separately and mix them to obtain plant extracts for later use;
[0032] The preparation method of the amaranth extract is as follows: crush the amaranth seeds, add 5 times the weight of water, sonicate (power 150W, frequency 30kHz) for 20 minutes, filter to obtain the residue, add 5 times the weight of 75% ethanol solution to the residue, reflux for 1 hour, filter to obtain the filtrate and residue, extract the residue again in the same way, obtain the filtrate again, combine the two filtrates, concentrate, dry and crush to obtain the amaranth extract;
[0033] The preparation method of the *Symplocos buergeriana* extract is as follows: *Symplocos buergeriana* stems and branches are crushed and then mixed with 1% lactic acid aqueous solution (3 times the weight of *Symplocos buergeriana*). The pH value is adjusted to 4.5, the temperature is raised to 45℃, and 0.5% (by weight of *Symplocos buergeriana*) cellulase and 0.5% (by weight of *Symplocos buergeriana*) pectinase are added. The mixture is stirred evenly and enzymatically hydrolyzed for 60 minutes. During the enzymatic hydrolysis, an electric field treatment is applied for 5 minutes (electric field strength of 40 kV / cm, pulse time of 600 μs, pulse frequency of 250 Hz) to obtain the enzymatic hydrolysate. The hydrolysate is filtered to obtain the filtrate, which is then freeze-dried and crushed to obtain the *Symplocos buergeriana* extract.
[0034] Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use.
[0035] Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
[0036] Example 2
[0037] A natural plant preparation for relieving animal stress, comprising, by weight, the following raw materials: 10 parts citrus; 5 parts green olive; 5 parts pine cone; 3 parts fire mulberry leaves; 3 parts poplar alkaloid; and 1 part plant extract; wherein the plant extract is a mixture of amaranth extract and sedge extract in a 1:1 weight ratio.
[0038] The method for preparing the natural plant preparation for alleviating animal stress includes the following steps:
[0039] Step 1: Prepare extracts of Amaranthus caudatus and Amaranthus spinosa separately and mix them to obtain plant extracts for later use;
[0040] The preparation method of the amaranth extract is as follows: crush the amaranth seeds, add 6 times the weight of water, sonicate (power 200W, frequency 40kHz) for 13 minutes, filter to obtain the residue, add 12 times the weight of 80% ethanol solution to the residue, reflux for 2 hours, filter to obtain the filtrate and residue, extract the residue again in the same way, combine the two filtrates, concentrate, dry and crush to obtain the amaranth extract;
[0041] The preparation method of the *Symplocos pubescens* extract is as follows: *Symplocos pubescens* stems are crushed and then 2% lactic acid aqueous solution (4 times the weight of *Symplocos pubescens*) is added. The pH value is adjusted to 5, the temperature is raised to 50℃, and 2% cellulase and 1% pectinase (by weight of *Symplocos pubescens*) are added. The mixture is stirred evenly and enzymatically hydrolyzed for 50 minutes. During the enzymatic hydrolysis, an electric field treatment (35kV / cm, pulse time 500μs, pulse frequency 200Hz) is applied for 6 minutes to obtain the enzymatic hydrolysate. The hydrolysate is filtered to obtain the filtrate, which is then freeze-dried and crushed to obtain the *Symplocos pubescens* extract.
[0042] Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use.
[0043] Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
[0044] Example 3
[0045] A natural plant preparation for relieving animal stress, comprising, by weight, the following raw materials: 20 parts citrus; 10 parts green olive; 0.5 parts pine cone; 0.05 parts fire mulberry leaf; 0.05 parts poplar alkaloid; 3 parts plant extract; and 1 part linoleic acid; wherein the plant extract is a mixture of amaranth extract and sedge extract in a weight ratio of 1:2.
[0046] The method for preparing the natural plant preparation for alleviating animal stress includes the following steps:
[0047] Step 1: Prepare extracts of Amaranthus caudatus and Amaranthus spinosa separately and mix them to obtain plant extracts for later use;
[0048] The preparation method of the amaranth extract is as follows: crush the amaranth seeds, add 10 times the weight of water, sonicate (power 300W, frequency 50kHz) for 5 minutes, filter to obtain the residue, add 20 times the weight of 90% ethanol solution to the residue, reflux for 3 hours, filter to obtain the filtrate and residue, extract the residue again in the same way, combine the two filtrates, concentrate, dry and crush to obtain the amaranth extract;
[0049] The preparation method of the *Symplocos pubescens* extract is as follows: *Symplocos pubescens* stems are crushed and then 5 times their weight of 4% lactic acid aqueous solution is added. The pH value is adjusted to 6, the temperature is raised to 50℃, and 3% of cellulase and 2% of pectinase by weight of *Symplocos pubescens* are added. The mixture is stirred evenly and enzymatically hydrolyzed for 40 minutes. During the enzymatic hydrolysis, an electric field treatment is applied for 10 minutes (electric field strength of 30kV / cm, pulse time of 400μs, pulse frequency of 150Hz) to obtain the enzymatic hydrolysate. The hydrolysate is filtered to obtain the filtrate, which is then freeze-dried and crushed to obtain the *Symplocos pubescens* extract.
[0050] Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use.
[0051] Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
[0052] Comparative Example 1
[0053] A natural plant preparation for relieving animal stress, by weight, comprises the following raw materials: 200 parts citrus; 10 parts green olive; 0.5 parts pine cone; 0.05 parts fire mulberry leaf; 0.05 parts poplar alkaloid; 3 parts plant extract; and 1 part linoleic acid; wherein all of the plant extract is amaranth extract.
[0054] The method for preparing the natural plant preparation for alleviating animal stress includes the following steps:
[0055] Step 1: Prepare amaranth extract as a plant extract for later use;
[0056] The preparation method of the amaranth extract is as follows: crush the amaranth seeds, add 10 times the weight of water, sonicate (power 300W, frequency 50kHz) for 5 minutes, filter to obtain the residue, add 20 times the weight of 90% ethanol solution to the residue, reflux for 3 hours, filter to obtain the filtrate and residue, extract the residue again in the same way, combine the two filtrates, concentrate, dry and crush to obtain the amaranth extract;
[0057] Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use.
[0058] Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
[0059] Comparative Example 2
[0060] A natural plant preparation for relieving animal stress, by weight, comprises the following raw materials: 20 parts citrus; 10 parts green olive; 0.5 parts pine cone; 0.05 parts fire mulberry leaf; 0.05 parts poplar alkaloid; 3 parts plant extract; and 1 part linoleic acid; wherein all of the plant extract is *Symplocos edulis* extract.
[0061] The method for preparing the natural plant preparation for alleviating animal stress includes the following steps:
[0062] Step 1: Prepare *Symplocos lanceolata* extract as a plant extract for later use;
[0063] The preparation method of the *Symplocos pubescens* extract is as follows: *Symplocos pubescens* stems are crushed and then 5 times their weight of 4% lactic acid aqueous solution is added. The pH value is adjusted to 6, the temperature is raised to 50℃, and 3% of cellulase and 2% of pectinase by weight of *Symplocos pubescens* are added. The mixture is stirred evenly and enzymatically hydrolyzed for 40 minutes. During the enzymatic hydrolysis, an electric field treatment is applied for 10 minutes (electric field strength of 30kV / cm, pulse time of 400μs, pulse frequency of 150Hz) to obtain the enzymatic hydrolysate. The hydrolysate is filtered to obtain the filtrate, which is then freeze-dried and crushed to obtain the *Symplocos pubescens* extract.
[0064] Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use.
[0065] Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
[0066] Performance testing:
[0067] I. Adrenocorticotropic hormone (ACTH) level test
[0068] A number of mice (half male and half female, weighing 18-22g, purchased from Chengdu Dashuo Experimental Animal Co., Ltd.) under the same feeding conditions were randomly divided into two groups: one group of 60 mice as stress mice and the other group of 15 mice as normal mice. All mice were placed in separate cages. All stress mice were placed at room temperature (25℃) and shaken continuously for 8 hours on a shaker at 180 times / min. Normal mice were simply placed at room temperature (25℃) without any treatment. The stress mice were randomly divided into 4 groups of 15 mice each. Immediately after the shaking, the mice were administered the preparations prepared in Example 3 (Group 1), Comparative Example 1 (Group 2), and Comparative Example 2 (Group 3), as well as physiological saline (Group 4). The normal mice were administered physiological saline at the same time (Group 5). The gavage volume was the same for all groups, 20ml / kg.
[0069] Blood samples were collected from the femoral vein at six time points: 2, 4, 8, 12, 24, and 36 hours after gavage. Five mice were collected from each group at each time point. Blood collection was performed by puncturing the femoral vein with an injection needle. Two blood samples (approximately 0.2–0.3 ml each) were collected from each mouse. One sample was injected into a tube pre-washed with 10% EDTA dipotassium and aprotinin, and the other into a tube pre-washed with heparin. Both samples were centrifuged at 4°C, 3000 rpm for 10 minutes to separate the plasma, and stored at -20°C for later use. Before analysis, frozen samples were thawed at room temperature or in cold water, centrifuged again at 4°C, 3000 rpm for 5 minutes, and the supernatant was used to determine adrenocorticotropic hormone (ACTH) levels. The assay was performed using the ELISAL kit (purchased from Beijing Northern Biotechnology Research Institute, stored at 2–8°C protected from light. Composition: ACTH standard (S0–S5)). 125 The following reagents were used: I-ACTH (red), rabbit anti-ACTH antiserum (blue), donkey anti-rabbit immunosorbent, and ACTH buffer (colorless).
[0070] The final adrenocorticotropic hormone (ACTH) level measurement results are shown below. Figure 1 As can be seen, in the first group, the ACTH concentration obtained by gavage using the natural plant preparation prepared in Example 3 was highest at 2 hours, but less than 20 pg / mL. Subsequently, the ACTH concentration gradually decreased, even falling below that of the fifth group at 36 hours. In the second and third groups, the ACTH concentration obtained by gavage using the natural plant preparations prepared in Comparative Examples 1 and 2, respectively, reached its highest point at 4 hours, significantly higher than that of the first group, as the preparations only contained extracts of *Amaranthus urinaria* and *Solanum lyratum*, respectively. The ACTH concentration gradually decreased thereafter, but remained as high as approximately 23 pg / mL at 36 hours. In the fourth group, the ACTH concentration obtained by gavage using physiological saline was the highest, exceeding 40 pg / mL at 4 hours. The fifth group, as a blank control, showed a stable ACTH concentration of approximately 15 pg / mL in normal mice. This demonstrates that the natural plant preparation obtained in this invention can avoid excessive activation of the hypothalamus-pituitary-adrenal axis and reduce the secretion and release of adrenocorticotropic hormone, thereby effectively alleviating stress responses.
[0071] II. Aquaculture Test
[0072] Fifty healthy domestic pigs aged 120 days were selected, numbered, weighed, and divided into 5 groups of 10 each, designated as groups 1-5. Group 1 was fed a basal diet containing the natural plant preparation prepared in Example 3; Group 2 was fed a basal diet containing the natural plant preparation prepared in Comparative Example 1; Group 3 was fed a basal diet containing the natural plant preparation prepared in Comparative Example 2; and Groups 4 and 5 were fed a basal diet with the plant preparation added at a ratio of 0.5% by weight.
[0073] The specific feeding methods are as follows: For groups 1-4, transportation stimulation is provided. Starting from the first day of feeding, the pigs are transported by truck on a bumpy road for 0.5 hours every morning; for group 5, no transportation stimulation is provided.
[0074] The five groups were raised under the same environmental conditions: the temperature in the pigsty was maintained at around 25℃, the relative humidity was maintained at around 70%, the pigs were raised on raised beds, and they had free access to feed and water. The manure was removed twice a day to keep the pigsty clean, and the mental state and number of pigs with diarrhea in each group were monitored and recorded at any time.
[0075] Fifteen days later, each pig was weighed, and the average daily feed intake, daily weight gain, and feed conversion ratio of each pig were calculated. The average value of each data point for each group was also calculated.
[0076] The calculation results are summarized in the following table:
[0077]
[0078] As can be seen from the table above, Group 1, which used the natural plant preparation prepared in Example 3 as a feed additive, allowed pigs to maintain good mental state and experience less diarrhea after transportation stress. Their average daily feed intake reached 2234.4g, average daily weight gain reached 912.3g, and the feed conversion ratio was controlled at 2.45. These figures are very close to those of Group 5, indicating that the use of the natural plant preparation significantly reduced the impact of transportation stress on pigs, to the point of being negligible. In contrast, Groups 2 and 3, which used only extracts of Amaranthus urinaria and Erigeron spinosa respectively in their preparations, showed significantly worse results. Their average daily feed intake decreased by 6.2% and 7.7% respectively, their average daily weight gain decreased by 7.4% and 8.8% respectively, and their feed conversion ratios also increased. This suggests that Amaranthus urinaria extract and Erigeron spinosa extract are most effective when used in combination.
[0079] The embodiments described above are merely examples of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.
Claims
1. A natural plant formulation for alleviating stress in animals, characterized in that, The raw materials for its preparation, by weight, include: Citrus fruits 0.1-20 parts; 0.1-10 parts green olives; 0.5-10 parts pine cones; 0.05-5 parts of mulberry leaves; Populus alkaloids 0.05-5 parts; Plant extracts 0.01-3 parts; The plant extract is composed of a mixture of Amaranthus caudatus extract and Erigeron burmannii extract in a weight ratio of 1:
2. The method for preparing the natural plant preparation for alleviating animal stress includes: Step 1: Prepare extracts of Amaranthus caudatus and Amaranthus spinosa separately and mix them to obtain plant extracts for later use. The preparation method of Amaranthus caudatus extract is as follows: Crush Amaranthus caudatus seeds, add 5-10 times their weight of water, sonicate for 5-20 minutes, filter to obtain residue, add 5-20 times their weight of 75-90% ethanol solution to the residue, reflux for 1-3 hours, filter to obtain filtrate and residue, extract the residue again in the same manner, combine the two filtrates, concentrate, dry, and crush to obtain the final extract. The preparation method of the Amaranthus urinaria extract and the Amaranthus spinosa extract is as follows: Pulverize the Amaranthus spinosa stems and add 3-5 times the weight of Amaranthus spinosa in a 1-4% lactic acid aqueous solution, adjust the pH to 4.5-6, heat to 45-50℃, add 0.5-3% of Amaranthus spinosa cellulase and 0.5-2% of Amaranthus spinosa pectinase by weight, stir evenly, and enzymatically hydrolyze for 40-60 minutes. During the enzymatic hydrolysis, apply an electric field for 5-10 minutes to obtain the enzymatic hydrolysate, filter to obtain the filtrate, freeze-dry and pulverize the filtrate to obtain the Amaranthus spinosa extract. Step 2: Take peeled citrus fruits, green olive fruits, mature pine cones, mulberry leaves, and poplar alkali crystals, remove impurities, wash them with clean water, dry them, mix them together, and crush them to obtain the main material slurry for later use. Step 3: While stirring, sprinkle the plant extract into the main ingredient slurry. After mixing evenly, sterilize and package to obtain the natural plant preparation.
2. The natural plant formulation for alleviating stress in animals as claimed in claim 1 wherein, The raw materials also include adjuvants, which account for 1-3% of the total weight of the preparation, and the adjuvants are linoleic acid, oleic acid or a mixture thereof.
3. The natural plant formulation for alleviating stress in animals as claimed in claim 1 wherein, The ultrasonic treatment has a power of 150-300W and a frequency of 30-50kHz.
4. A natural plant preparation for relieving animal stress according to claim 1, characterized in that, The electric field strength of the electric field treatment is 30-40 kV / cm, the pulse time is 400-600 μs, and the pulse frequency is 150-250 Hz.