Universal slide holder

By designing a universal slide holder that uses a negative pressure groove and a sealing ring to adsorb slides, the problems of slide damage and residue are solved, enabling the reuse and cleaning of slides, reducing costs, and improving stability and experimental accuracy.

CN115261230BActive Publication Date: 2026-07-14PEKING UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
PEKING UNIV
Filing Date
2022-09-01
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

The glass slides in existing petri dishes are easily damaged or have sample residue, which affects the experimental results. In addition, the petri dishes have low stability and are prone to drifting.

Method used

Design a universal slide holder that uses a negative pressure groove and a sealing ring to firmly attach the slide to the device body. The slide is integrated with the chamber to achieve rapid loading, unloading, cleaning and disinfection. The use of metal material increases stability and is equipped with a liquid exchange assembly for live cell culture.

Benefits of technology

This enables the reuse and cleaning of glass slides, reduces experimental costs, improves the stability of microscopic observation, reduces waste of consumables, and ensures the accuracy of experimental results.

✦ Generated by Eureka AI based on patent content.

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    Figure CN115261230B_ABST
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Abstract

The present application relates to a kind of universal glass holder, device main body has the placement plane for placing glass;Chamber is opened in device main body, chamber is used to place sample, the cavity mouth of chamber is located in the placement plane of device main body;Negative pressure groove is opened in device main body, the slot of negative pressure groove is located in the placement plane of device main body;Vacuum interface is opened in device main body, vacuum interface is communicated with negative pressure groove, for connecting negative pressure generating device.The above-mentioned glass holder, through negative pressure groove and sealing ring, tightly adsorbs glass on the placement plane of device main body, and glass is combined with chamber to constitute culture dish.Through the cooperation of vacuum interface and negative pressure groove, the quick loading and unloading of glass can be achieved, so that new glass can be replaced at any time after glass damage, the influence on subsequent experiment is avoided, and the intact, clean glass can be guaranteed.
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Description

Technical Field

[0001] This invention relates to the field of experimental instrument technology, and in particular to a universal slide holder. Background Technology

[0002] Slide holders are commonly used laboratory equipment. In the lab, petri dishes are frequently used to place samples for observation under a microscope. The part of these petri dishes that actually holds the sample and contacts the objective lens is the slide; the other parts may only be contaminated with some liquid. Theoretically, the entire petri dish can be washed, sterilized, and reused repeatedly. However, after washing, the slide may be damaged, such as scratched, and sample residue may remain, affecting subsequent experimental results.

[0003] Existing petri dishes, such as Pietro plates, are one-piece units. If the same dish is to be reused for observing other samples after an experiment, damage to the slide or the presence of difficult-to-remove sample residue can significantly impact subsequent experimental results, leading to data deviation. Furthermore, the lightweight nature of the petri dish makes it less stable under a microscope, prone to drift. Therefore, disassembling the petri dish into a chamber and a slide to ensure the slide remains intact and clean during reuse is a pressing issue. Summary of the Invention

[0004] Therefore, it is necessary to provide a universal slide holder to address the issue of how to keep slides intact and clean when reusing culture dishes in experiments.

[0005] A universal slide holder, comprising:

[0006] The main body of the device has a flat surface for placing glass slides;

[0007] A chamber is formed on the device body and is used to place a sample. The opening of the chamber is located on the object placement plane of the device body.

[0008] The negative pressure groove is formed on the main body of the device, and the groove opening is located on the placement plane of the main body of the device.

[0009] The vacuum interface is located on the main body of the device and is connected to the negative pressure tank for connecting the negative pressure generating device.

[0010] In this embodiment, the glass slide is tightly adsorbed onto the placement plane of the device body by a negative pressure groove and a sealing ring, and the glass slide and chamber together form a petri dish. The vacuum interface and negative pressure groove allow for rapid loading and unloading of the glass slide, enabling it to be reused after the experiment by simply replacing the slide and cleaning and sterilizing the entire chamber. Slides containing other samples can also be placed on the chamber to form petri dishes for new experimental observations. The experimental consumables have been changed from petri dishes to glass slides, significantly reducing experimental costs. Furthermore, if the glass slide holder body is made of metal, its weight can be increased, effectively improving stability and reducing drift when placed under a microscope.

[0011] In one embodiment, the chamber is a through-hole structure that passes through the main body of the device.

[0012] In this embodiment, the main body of the device is a hexahedron. The hexahedron has a stable structure, which can improve stability and reduce drift when placed on a microscope for observation. The chamber is a through hole on two sides. When in use, the opening near the object placement plane is covered by a glass slide, and the other side is open to facilitate microscope observation. The chamber stores experimental samples, and the objective lens is placed in the opening of the chamber away from the glass slide for observation, which can avoid the objective lens from being inserted into the chamber for observation and colliding with the chamber.

[0013] In one embodiment, the number of negative pressure grooves is at least two, and the at least two negative pressure grooves are respectively arranged on both sides of the chamber.

[0014] In this embodiment, the negative pressure groove is used to form a negative pressure space and generate a pressure difference with the external air pressure, so that the glass slide is firmly adsorbed in the glass slide groove of the device body; the negative pressure grooves respectively set on both sides of the chamber generate adsorption forces on both ends of the glass slide, so that the glass slide is subjected to more uniform force and the adsorption is more stable, and the glass slide will not be broken or damaged due to uneven force.

[0015] In one embodiment, the device body has a connecting slot, and at least two negative pressure slots are connected through the connecting slot.

[0016] In this embodiment, the connecting groove connects the negative pressure grooves distributed at both ends of the chamber, making the negative pressure in the two negative pressure grooves equal and generating equal adsorption forces; at the same time, the other end of the connecting groove is connected to a vacuum port, so that the negative pressure generating device can generate a negative pressure environment in the negative pressure groove through the vacuum port and the connecting groove; the connecting groove connects the two negative pressure grooves, so that only one vacuum port and a negative pressure generating device are needed to make the two negative pressure grooves generate negative pressure simultaneously.

[0017] In one embodiment, a sealing groove is provided on the placement plane, and a sealing element is provided in the sealing groove. The sealing groove is an annular groove structure, the sealing groove surrounds the negative pressure groove, and the opening of the sealing groove is located on the placement plane of the device body.

[0018] In this embodiment, a sealing groove is arranged around the chamber and the negative pressure groove. The sealing groove surrounding the chamber is used to seal the chamber, so that the experimental liquid injected into the chamber will not flow out during the experiment, and the experimental liquid in the chamber will not be contaminated, thus avoiding affecting the experimental results. The sealing groove surrounding the negative pressure groove is used to seal the negative pressure groove, so that when the negative pressure environment is generated, air leakage will not weaken or even disappear the negative pressure environment of the negative pressure groove. It also prevents the glass slide from falling off the placement surface due to air leakage, thus affecting the experimental results.

[0019] In one embodiment, the seal is a silicone ring.

[0020] In this embodiment, a silicone ring is used as the sealing element. The silicone ring has excellent sealing performance, is waterproof and leak-proof, and also has the characteristics of being resistant to high and low temperatures, not deforming, not producing chemical reactions, and not producing harmful substances. Using a silicone ring as the sealing element can maintain good sealing performance without contaminating the experimental sample or producing a chemical reaction with the experimental sample.

[0021] In one embodiment, it includes:

[0022] The fluid exchange assembly is connected to the chamber.

[0023] In this embodiment, the structure of this application can not only be used for ordinary observation experiments, but also be equipped with a liquid exchange component to serve as a live cell culture device for culturing live cells. This embodiment is a preferred embodiment. As a live cell culture device, the negative pressure adsorption device of this application can be used to conveniently replace glass slides, so that the live cell culture device can remain clean after each cleaning, avoiding affecting secondary use.

[0024] In one embodiment, the fluid exchange assembly includes an inlet pipe and an outlet pipe, both of which are in communication with the chamber.

[0025] In this embodiment, the medium exchange assembly includes an inlet pipe and an outlet pipe. The inlet pipe is used to deliver the nutrient solution, and the outlet pipe is responsible for discharging the used waste liquid. The medium exchange assembly is connected to the chamber, allowing the inlet pipe to deliver the nutrient solution into the chamber to provide nutrition for the culture of live cells, and the outlet pipe to discharge the used waste liquid, facilitating the re-injection of new nutrient solution by the inlet pipe. The inlet and outlet pipes work together to enable convenient replacement of the nutrient solution in the live cell culture device, ensuring that the live cells are always in a sufficiently nutrient-rich environment during the culture process.

[0026] In one embodiment, the device body has two through holes, both of which are connected to the chamber. The inlet pipe and the outlet pipe are connected to the chamber through the two through holes respectively.

[0027] In this embodiment, two through holes with dimensions matching the liquid inlet and liquid outlet pipes are opened on the side adjacent to the placement plane of the device body. The liquid inlet and liquid outlet pipes extend into the two through holes and are connected to the chamber, so that the liquid inlet pipe can deliver new nutrient solution into the chamber and the liquid outlet pipe can deliver waste liquid out of the chamber.

[0028] In one embodiment, the device body is a regular hexahedron, the placement plane is one of the surfaces of the device body, and the through hole is formed on the surface of the device body adjacent to the placement plane.

[0029] In this embodiment, the through-hole for setting the inlet and outlet pipes begins on the surface adjacent to the placement plane, which is also the side outside the non-opening sides of the chamber. This through-hole facilitates the insertion of the fluid exchange assembly into the chamber from the side. Attached Figure Description

[0030] Figure 1 This is a structural diagram of the slide holder of the present invention.

[0031] Figure 2 This is a partial cross-sectional view of the glass slide holder of the present invention.

[0032] Figure 3 This is a diagram of an embodiment of the slide holder of the present invention.

[0033] Figure 4 This is a diagram of the liquid exchange tube in one embodiment of the slide holder of the present invention.

[0034] Figure 5 This is a detailed view of the liquid exchange tube of the slide holder of the present invention.

[0035] Figure label:

[0036] 1- Device body;

[0037] 2-chamber;

[0038] 3-Negative pressure groove;

[0039] 4-Sealing ring;

[0040] 5-Vacuum interface;

[0041] 6-Connecting slot;

[0042] 7-Connecting slot and negative pressure slot through-hole;

[0043] 8-Through hole;

[0044] 9- Fluid changing tube. Detailed Implementation

[0045] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0046] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0047] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0048] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0049] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0050] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0051] This application provides a universal slide holder. Specifically, it can be a negative pressure adsorption slide holder composed of a device body 1, a chamber 2, a negative pressure groove 3, and a vacuum interface 5. The slide holder will be analyzed in detail below with reference to the accompanying drawings.

[0052] Specifically, in one embodiment of the present invention, such as Figure 1 The main body 1 of the device shown is a hexahedral solid, with one side for holding the glass slide serving as the placement plane. A slide groove is formed on the placement plane, the size, shape, and dimensions of which correspond to the glass slide to be held. The depth of the slide groove is equal to the thickness of the glass slide, which is typically less than 2 mm. In experiments, due to aberration correction in the objective lens design, the slide thickness is marked on the objective lens. Most often, a 0.17 mm coverslip is used to directly contact the objective lens for observation. After the glass slide is firmly held in place by negative pressure, the surface of the glass slide near the placement plane is aligned with the placement plane. Optionally, the material of the main body 1 is not limited; it can be any material that meets experimental requirements and will not affect the experimental sample, such as metal or plastic. Metals can be iron, aluminum, alloys, etc. Optionally, in a preferred embodiment, the shape of the main body 1 is not limited; it can be any shape capable of holding and stably placing the glass slide, such as a cylinder or hexahedron. Optionally, the size of the device body 1 is not limited and can be any size that meets the requirements for clamping the glass slide.

[0053] Specifically, in one embodiment of the present invention, such as Figure 1 The chamber 2 shown is disposed on the main body 1 of the device, penetrating through the object placement plane and facing it. The chamber is used to place experimental samples. In use, one end of the chamber near the object placement plane is sealed with a glass slide, while the other end remains open. Optionally, the shape of the chamber is not limited and can be any shape that facilitates the placement of experimental samples, such as quadrilaterals, circles, etc.

[0054] Specifically, in one embodiment of the present invention, such as Figure 1The negative pressure grooves 3 shown are formed on the main body 1 of the device, with at least two negative pressure grooves 3 located on both sides of the chamber 2, symmetrically distributed. The opening of the negative pressure groove 3 is located on the placement plane of the main body 1, and the other end is sealed. In use, the negative pressure generating device evacuates the negative pressure groove to create a vacuum environment, forming a negative pressure adsorption of the glass slide, so that the glass slide is firmly fixed in the glass slide groove of the main body 1. Optionally, in a preferred embodiment of the present invention, the number of negative pressure grooves 3 is not limited, and can be any number that can firmly adsorb the glass slide, such as: 1, 2, 3, etc. Optionally, in a preferred embodiment of the present invention, the size of the negative pressure grooves 3 is not limited, and can be any size that can generate a sufficient pressure difference with the outside to firmly adsorb the glass slide. Optionally, in a preferred embodiment of the present invention, the shape of the negative pressure grooves 3 is not limited, and can be any shape with sufficient adsorption area to firmly adsorb the glass slide, such as: rounded quadrilateral, circle, etc. Optionally, in a preferred embodiment of the present invention, the position of the negative pressure grooves 3 is not limited, and can be any position that can firmly adsorb the glass slide.

[0055] Specifically, in one embodiment of the present invention, the vacuum port 5 is disposed on the side adjacent to the placement plane of the device body 1. The vacuum port 5 is used in conjunction with the negative pressure generating device to generate negative pressure in the negative pressure groove 3 through the connecting groove 6, thereby adsorbing the glass slide. Optionally, in a preferred embodiment of the present invention, the position of the vacuum port 5 is not limited and can be any position that can connect to the connecting groove 6.

[0056] Specifically, in one embodiment of the present invention, the connecting groove 6 is disposed inside the device body 1, and the connecting groove 6 is connected to the negative pressure groove 3, with the other end connected to the vacuum port 5. In use, the air inside the negative pressure groove 3 is drawn out by the negative pressure generating device and flows through the connecting groove 6 to the vacuum port 5. After the air inside the negative pressure groove 3 is evacuated through the connecting groove 6, a negative pressure vacuum environment is formed, creating a pressure difference with the external atmospheric pressure, which firmly adheres and fixes the glass slide. Optionally, in a preferred embodiment of the invention, the position of the connecting groove 6 is not limited and can be any position connecting the negative pressure groove 3 and the vacuum port 5; correspondingly, the size of the groove opening of the connecting groove 6 is not limited and can be any size that does not affect the normal operation of the negative pressure device.

[0057] Specifically, in one embodiment of the present invention, a sealing ring 4 is provided in the slide groove of the device body 1. The sealing ring 4 is specifically disposed around the opening of the negative pressure groove 3 and around the opening of the chamber 2 near the slide. It is used to seal the edge of the contact between the negative pressure groove and the slide when the slide is adsorbed under negative pressure, preventing air leakage that could lead to unstable negative pressure adsorption. It also serves to maintain a seal when the sample is placed in the chamber 2, preventing the sample from flowing out from the end of the chamber 2 near the slide and affecting the experimental results. Optionally, in a preferred embodiment of the present invention, the sealing ring 4 can be any sealing device, such as a rubber ring.

[0058] Specifically, in one embodiment of the present invention, a through port 7 for connecting the connecting groove and the negative pressure groove is provided, which is located at one end of the negative pressure groove 3 near the connecting groove 6, for connecting the negative pressure groove 3 and the connecting groove 6.

[0059] This application provides a universal slide holder that fixes the slide to the chamber by forming a negative pressure adsorption through a negative pressure groove. This slide holder is suitable for slides of any shape.

[0060] In a preferred embodiment of this application, a fluid exchange tube can also be added for use in a live cell culture apparatus. Specifically, as shown... Figure 3 As shown, two through holes 8 are made on the side of the device body 1 where the placement plane and the vacuum interface 5 are adjacent, as shown. Figure 4 As shown, the fluid exchange pipe is disposed in the through hole 8; specifically, in one embodiment, the fluid exchange pipe includes an inlet pipe and an outlet pipe.

[0061] The slide holder described in this application has no restrictions on the shape of the main body of the device, and therefore the shape of the slide groove set on the placement plane of the main body is also unrestricted. In experiments, circular slides (φ24mm, φ20mm, φ14mm, φ8mm) and rectangular slides (25×75mm) are usually used. Different slides usually require slide holders of different sizes and shapes, which can greatly affect the experiment. The slide holder described in this application can firmly adsorb slides of any size and shape through negative pressure adsorption, which is more versatile and more convenient.

[0062] The slide holder described in this application adopts a separate slide and chamber design. Compared with those culture dishes where the chamber and the bottom slide are integrated, after one experiment, there is no need to discard the entire culture dish. Only the slide needs to be replaced to conduct the next experiment, which greatly reduces the waste of consumables and saves experimental costs. At the same time, when a new experiment is conducted after replacing the slide, the new slide will not have any traces left from the previous experiment, so that the new experiment will not be affected.

[0063] In Example 1, the main body of the device is a hexahedron, and the cavity is a through hole with a rectangular cross-section, one of which has an opening located on the placement plane. A rounded rectangular negative pressure groove is provided on each side of the cavity, and the two negative pressure grooves are symmetrically distributed with respect to the axis of symmetry of the rectangular cross-section cavity.

[0064] The negative pressure groove is open at one end near the surface and closed at the other. A portion of it near the connecting groove has an opening that connects to the connecting groove. The two negative pressure grooves are interconnected via the connecting groove, ensuring equal air pressure inside each groove. When a negative pressure environment is created inside the groove, the adsorption force exerted on the glass slide by the two grooves is equal, resulting in uniform and balanced force on the slide, allowing it to be more firmly adsorbed onto the surface.

[0065] A sealing groove is provided on the side of the cavity near the placement surface, surrounding the cavity opening. A sealing element, a silicone ring, is installed inside the sealing groove. During use, the glass slide is firmly adsorbed onto the placement surface, and the silicone ring contacts the glass slide, acting as a seal to isolate the inside and outside of the cavity. This prevents the experimental sample liquid placed in the cavity from flowing out from the opening on the side near the placement surface during the experiment, thus avoiding contamination. At the same time, the silicone ring has the characteristics of not easily undergoing chemical reactions and not easily deteriorating or deforming, which can prevent the experimental sample liquid from undergoing a chemical reaction after contacting the sealing strip, thereby affecting the experimental results.

[0066] The connecting slot has two openings on its side, each connecting to a negative pressure slot. A vacuum interface is located on the side of the device body adjacent to the placement plane, connecting the connecting slot to the vacuum interface. The vacuum interface consists of two through holes of different sizes. The first through hole, farther from the device body, is smaller and matches the size of the connecting slot opening, allowing the connecting slot opening to connect with this first through hole. The second through hole, closer to the device body surface, is larger and matches the size of the negative pressure generating device interface, making it easier and more secure to install the negative pressure generating device into the vacuum interface.

[0067] In this embodiment, the glass slide is pressed from the surface of the placement plane towards the surface opposite to the placement plane, placing it in the slide groove of the placement plane. The slide is then held firmly against the sealing silicone ring, and the negative pressure generating device is activated, creating a negative pressure environment in the negative pressure groove. This creates a pressure difference with the external atmospheric pressure, firmly adhering the slide to the slide groove of the placement plane. With the slide securely held, experimental sample liquid can be injected through another opening in the cavity. The slide holder is then placed on a microscope for experimental observation. After the experiment, the negative pressure generating device is turned off, allowing the air pressure in the negative pressure groove to return to atmospheric pressure. The slide then loses its adhesive force and falls off. At this point, only the slide needs to be replaced and the slide holder cleaned to continue the experiment. The only consumable is the glass slide, greatly reducing the time required for slide replacement, improving experimental efficiency, and also reducing experimental costs.

[0068] In Example 2, two through holes are provided on one surface adjacent to the device body and the placement plane. One opening of the through hole is on the surface of the device body, and the other opening is on the inner surface of the cavity. In this example, a liquid exchange assembly is provided, which includes an inlet pipe and an outlet pipe, which are connected to the inside and outside of the cavity through the through holes respectively. The inlet pipe is used to transport the nutrient solution into the cavity, and the outlet pipe is responsible for removing the waste liquid from the inside of the cavity.

[0069] After the glass slide is firmly adsorbed by the negative pressure tank, the opening on the side of the cavity near the placement plane is sealed, forming a space with only one opening inside the cavity. When combined with the liquid exchange assembly, it can be transformed into a live cell culture device.

[0070] The cavity for holding live cells and culture medium is open at one end and sealed with a glass slide at the other end, allowing for convenient and clear observation from both the top and bottom. It is also equipped with inlet and outlet tubes, which ensure that the cultured live cells are not affected while easily changing the nutrient solution, keeping the live cells in a suitable environment at all times.

[0071] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0072] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A universal glass slide holder, characterized in that, include: The device body has a placement plane for placing a glass slide; A chamber is formed on the main body of the device and is used to place a sample. The opening of the chamber is located on the placement plane of the main body of the device. The chamber extends through the placement plane and is face to face with the placement plane. In use, one end of the chamber near the placement plane is sealed by a glass slide, while the other end remains open. The glass slide and the chamber together form a culture dish. An objective lens is placed in the chamber away from the opening of the glass slide for observation. A negative pressure groove is formed on the main body of the device, and the groove opening is located on the placement plane of the main body of the device; A vacuum port is provided on the main body of the device and is connected to the negative pressure groove for connecting a negative pressure generating device. A fluid exchange assembly is connected to the chamber; the fluid exchange assembly includes an inlet pipe and an outlet pipe, both of which are connected to the chamber.

2. The slide holder according to claim 1, characterized in that, The cavity is a through-hole structure that penetrates the main body of the device.

3. The slide holder according to claim 1, characterized in that, The number of negative pressure grooves is at least two, and the at least two negative pressure grooves are respectively arranged on both sides of the chamber.

4. The slide holder according to claim 3, characterized in that, The main body of the device has a connecting groove, and at least two of the negative pressure grooves are connected through the connecting groove.

5. The slide holder according to claim 1, characterized in that, A sealing groove is provided on the placement plane, and a sealing element is provided in the sealing groove. The sealing groove is an annular groove structure, and the sealing groove surrounds the negative pressure groove. The opening of the sealing groove is located on the placement plane of the device body.

6. The slide holder according to claim 5, characterized in that, The sealing element is a silicone ring.

7. The slide holder according to claim 1, characterized in that, The device body has two through holes, both of which are connected to the chamber. The liquid inlet pipe and the liquid outlet pipe are connected to the chamber through the two through holes respectively.

8. The slide holder according to claim 7, characterized in that, The main body of the device is a hexahedron, the placement plane is one of the surfaces of the main body of the device, and the through hole is opened on the surface of the main body of the device adjacent to the placement plane.