Apparatus and method for detecting gas generation rate

Abstract

The application provides a device and method for detecting gas generation speed, which comprises a master control device, a gas washing device, a gas detection device and a temperature detection device; the gas washing device is used for washing a gas pipeline and discharging generated gas; the temperature detection device is used for detecting the current temperature of the generated gas; the gas detection device is used for identifying the type of the generated gas, detecting the gas proportion of different gases and the pressure change difference before and after the generated gas; the master control device is provided with a gas production speed calculation model, which is used for calculating the generation speed of different gases according to the gas proportion detected by the gas detection device and the pressure change difference before and after the generated gas, in combination with the current temperature, within a measurement time. The device for detecting gas generation speed provided by the application embodiment can accurately and quickly realize real-time detection of the generation speed of various gases, improve the detection accuracy of the gas generation speed and reduce the detection cost.

Description

Technical Field

[0001] This application relates to the field of gas detection technology, and in particular to a device and method for detecting the rate of gas generation. Background Technology

[0002] Currently, the detection methods for gases generated by microorganisms are mostly concentrated on spectroscopic detection methods, pressure detection methods, and mass spectrometry detection methods in the reaction system.

[0003] However, spectroscopic and mass spectrometric detection methods are costly and require bulky equipment, making them unsuitable for lightweight gas detection and collection devices. Pressure detection methods can only obtain the total amount of gas generated during microbial reactions, but cannot accurately identify the various gases produced or determine their molar mass. Therefore, current methods for detecting gas generation rates from microorganisms suffer from low accuracy and high cost. Furthermore, existing gas generation rate detection devices lack direct and efficient control mechanisms, making them unsuitable for various scenarios in biological experiments, such as fermentation efficiency assessment in brewing and fermentation experiments. Summary of the Invention

[0004] This application provides an apparatus and method for detecting gas generation rate, which aims to improve the detection accuracy and efficiency of gas generation rate through effective control and calculation models, and reduce detection costs.

[0005] In a first aspect, this application provides a device for detecting the gas generation rate, including a main control device, a gas scrubbing device, a gas detection device, and a temperature detection device;

[0006] The gas scrubbing device is used to clean the gas pipeline and discharge the generated gas.

[0007] The temperature detection device is used to detect the current temperature of the generated gas;

[0008] The gas detection device is used to identify the type of gas generated, and to detect the proportion of different gases and the pressure difference before and after gas generation.

[0009] The main control device is used to control the gas washing device, the gas detection device, and the temperature detection device. The main control device has a built-in gas production rate calculation model, which is used to calculate the generation rate of different gases within the measurement time based on the gas percentage detected by the gas detection device and the pressure change difference before and after gas generation, combined with the current temperature of the generated gas detected by the temperature detection device.

[0010] According to the present application, a device for detecting the rate of gas generation is provided, wherein the gas detection device includes a variety of gas molecule sensors and a pressure sensing device;

[0011] The multiple gas molecule sensors are used to identify the types of generated gases and detect the proportion of different gases. The multiple gas molecule sensors are placed in series on the gas pipeline, with one end connected to the internal environment of the device for detecting the gas generation rate and the other end connected to the external atmospheric environment.

[0012] The pressure sensing device is used to detect the pressure difference before and after the gas is generated.

[0013] According to the present application, a device for detecting the gas generation rate is provided, wherein the pressure sensing device includes a hydraulic U-tube and a liquid level sensor placed in the hydraulic U-tube and connected to the atmosphere at the end thereof;

[0014] The hydraulic U-tube contains a light liquid, with one end connected to the external atmospheric environment and the other end connected to the internal environment of the device for detecting the gas generation rate. The pressure change difference is obtained by detecting the liquid level difference before and after gas generation through the liquid level sensor.

[0015] According to the device for detecting the gas generation rate provided in this application, the main control device obtains the current temperature of the gas generated during the gas measurement process, the type of gas generated, the proportion of different gases, and the pressure change difference before and after gas generation through a digital communication protocol port, so as to calculate the generation rate of different gases; the main control device includes a single-chip microcomputer main control device.

[0016] According to the present application, a device for detecting the gas generation rate is provided, wherein the gas washing device includes a gas storage tank and a power unit; the gas storage tank stores inert gas; the main control unit controls the power unit to drive the inert gas in the gas storage tank to clean the gas pipeline and discharge the gas generated during the fermentation process.

[0017] According to the device for detecting the gas generation rate provided in this application, the gas storage tank is configured with a piston-propulsion structure, and the piston in the gas storage tank is pushed by the power device to drive the inert gas to clean the gas pipeline; the power device includes a stepper motor.

[0018] According to the present application, a device for detecting the gas generation rate is provided, wherein the gas generation reaction device is a biological fermentation tank; the number of biological fermentation tanks is at least one, and the inlet and outlet of the biological fermentation tank are provided with electromagnetic valves; the temperature detection device is provided at the outlet of the biological fermentation tank.

[0019] According to the present application, a device for detecting gas generation rate is provided, wherein there is one bio-fermentation tank, which is connected to the gas washing device and the gas detection device through a gas pipeline, and multiple solenoid valves are installed at the inlet and outlet of the gas pipeline; the gas washing device and the pressure sensing device are located on the inlet side of the bio-fermentation tank; and the multiple gas molecule sensor device is located on the outlet side of the bio-fermentation tank.

[0020] Secondly, this application provides a method for detecting the gas generation rate, wherein the method for detecting the gas generation rate is applied to the device for detecting the gas generation rate, comprising:

[0021] The gas scrubbing device is controlled to pressurize inert gas into the gas pipeline to clean the internal environment of the device for detecting the gas generation rate.

[0022] When the rate of gas generation is measured, the solenoid valve is switched on and off and the gas detection device is controlled to identify the types of gases generated during fermentation, as well as to detect the proportion of different gases and the pressure difference before and after gas generation.

[0023] The generation rate of different gases is calculated based on the proportion of different gases, the pressure difference before and after gas generation, and the current temperature of the generated gases.

[0024] In one embodiment, the step of calculating the generation rate of different gases based on the gas proportions and the pressure difference before and after gas generation, combined with the current temperature of the generated gases, includes:

[0025] Based on the proportion of different gases and the pressure difference before and after gas generation, combined with the density of the light liquid in the hydraulic U-tube, the inner diameter of the hydraulic U-tube, the current temperature, and the internal environmental volume of the device for detecting gas generation rate, the generation rate of different gases is calculated using a gas production rate calculation model.

[0026] Thirdly, this application provides a gas production rate detection device, comprising:

[0027] The cleaning control module is used to control the gas scrubbing device to pressurize inert gas into the gas pipeline to clean the internal environment of the device for detecting the gas generation rate.

[0028] The control and detection module is used to control the solenoid valve to switch on and off and control the gas detection device to identify the types of gases generated during fermentation when the gas generation rate is first measured, as well as to detect the proportion of different gases and the pressure change difference before and after gas generation.

[0029] The velocity measurement module is used to calculate the generation rate of different gases based on the proportion of different gases, the pressure difference before and after gas generation, and the current temperature of the generated gas.

[0030] Fourthly, this application also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the program to implement the gas production rate detection method described in the second aspect.

[0031] Fifthly, this application also provides a computer program product, which includes a computer program that, when executed by the processor, implements the gas production rate detection method described in the second aspect.

[0032] In a sixth aspect, this application also provides a non-transitory computer-readable storage medium comprising a computer program that, when executed by the processor, implements the gas production rate detection method of the second aspect.

[0033] The device and method for detecting gas generation rate provided in this application, during the gas generation rate detection process, utilize a main control device to precisely control the solenoid valves before, during, and after the reaction, and a gas detection device to quickly and accurately read the generation rates of various gases during the reaction process, thereby improving the accuracy of gas generation rate detection. Furthermore, the device for detecting gas generation rate provided in this application features simple and low-cost assembly of the various components, thus reducing the cost of gas generation rate detection. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in this application or the prior art, the accompanying drawings in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0035] Figure 1 This is a schematic diagram of the device for detecting gas generation rate provided in this application;

[0036] Figure 2 This is a schematic diagram of an application scenario of an embodiment of the device for detecting gas generation rate provided in this application;

[0037] Figure 3 This is a schematic diagram of the pre-reaction pipeline cleaning process provided in this application;

[0038] Figure 4This is a schematic diagram illustrating the generation of hydraulic pressure difference during the testing process provided in this application;

[0039] Figure 5 This is a structural schematic diagram of the post-reaction pipeline cleaning process provided in this application;

[0040] Figure 6 This is a flowchart illustrating the method for detecting gas generation rate provided in this application. Detailed Implementation

[0041] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0042] In the description of the embodiments of this application, it should be noted that the terms "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application. In addition, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0043] In the description of the embodiments of this application, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this application based on the specific circumstances.

[0044] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the embodiments of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0045] Combination Figures 1 to 6 This application describes the apparatus and method for detecting the rate of gas generation. Figure 1 This is a schematic diagram of the device for detecting gas generation rate provided in this application; Figure 2 This is a schematic diagram of an application scenario of an embodiment of the device for detecting gas generation rate provided in this application; Figure 3 This is a schematic diagram of the pre-reaction pipeline cleaning process provided in this application; Figure 4 This is a schematic diagram illustrating the generation of hydraulic pressure difference during the testing process provided in this application; Figure 5 This is a structural schematic diagram of the post-reaction pipeline cleaning process provided in this application; Figure 6 This is a flowchart illustrating the method for detecting gas generation rate provided in this application.

[0046] This application provides embodiments of a device for detecting the rate of gas generation. It should be noted that although a logical order is shown in the flowchart, under certain data conditions, the steps shown or described may be performed in a different order than that shown here.

[0047] Reference Figure 1 , Figure 1 This is a schematic diagram of the device for detecting gas generation rate provided in this application. The device for detecting gas generation rate provided in this application includes a main control device 10, a gas scrubbing device 20, a temperature detection device 30, and a gas detection device 40;

[0048] The gas washing device 20 and the gas detection device 40 are connected to the gas generating reaction device through a gas pipeline, and the gas pipeline is equipped with multiple solenoid valves at the inlet and outlet.

[0049] The gas scrubbing device 20 is used to clean the gas pipeline and discharge the generated gas;

[0050] The temperature detection device 30 is used to detect the current temperature of the generated gas;

[0051] The gas detection device 40 is used to identify the type of gas generated, and to detect the proportion of different gases and the pressure change difference before and after the gas is generated.

[0052] The main control device 10 is used to control the gas washing device 20, the temperature detection device 30, the gas detection device 40, and multiple solenoid valves. The main control device 10 is equipped with a gas production rate calculation model, which is used to calculate the generation rate of different gases within a measurement time based on the gas ratio detected by the gas detection device 40 and the pressure change difference before and after gas generation, combined with the current temperature of the generated gas detected by the temperature detection device 30.

[0053] It should be noted that in this embodiment, the main control device 10 communicates with the gas scrubbing device 20, multiple solenoid valves, temperature detection device 30, and gas detection device 40 via wired or wireless communication. Wired communication includes, but is not limited to, port communication and wired connection communication; wireless communication includes, but is not limited to, Bluetooth communication and wireless local area network communication. Furthermore, the main control device 10 includes a display that can show the on / off status, type, data, and connection relationship of the solenoid valves, gas scrubbing device 20, temperature detection device 30, and gas detection device 40. Furthermore, the gas scrubbing device 20 and gas detection device 40 are connected to the gas-generating reaction device via gas pipelines, and multiple solenoid valves are installed at the inlet and outlet of the gas pipelines.

[0054] Furthermore, the solenoid valve can be a switching device, so it can be understood that the main control device 10 can control the opening or closing of multiple solenoid valves to control the opening or closing of the gas inlet and outlet of the gas pipeline, thereby controlling the connection status between the gas washing device 20, the gas detection device 40 and the gas generating reaction device.

[0055] Furthermore, the gas-generating reaction device can carry out a fermentation reaction to generate gas, and a temperature detection device 30 is installed at the gas outlet of the gas-generating reaction device to detect the current temperature of the generated gas.

[0056] Furthermore, the gas scrubbing device 20 is a driving device, so it can be understood that the main control device 10 can control the driving of the gas scrubbing device 20, clean the gas pipeline and discharge the generated gas.

[0057] Furthermore, the gas detection device 40 includes multiple gas molecule sensors and a pressure sensing device. The pressure sensing device is equipped with a liquid level sensor. Therefore, it can be understood that the main control device 10 can obtain the liquid level difference between the two ends of the pressure sensing device through the liquid level sensor; and identify the type of gas generated and the proportion of different gases through multiple gas molecule sensors.

[0058] Furthermore, the main control device 10 includes a gas production rate calculation model. This model can calculate the production rate of different gases within the measurement time based on the gas percentage detected by the gas detection device 40, the pressure difference before and after gas generation, and the current temperature of the generated gas detected by the temperature detection device 30. Specifically: the temperature detection device 30 detects the current temperature of the generated gas during the gas production rate measurement process; the liquid level sensor at the end of the gas detection device 40 (liquid U-tube) connected to the atmospheric environment can read the hydraulic pressure difference between the two ends of the liquid U-tube, thereby determining the pressure difference before and after gas generation; the gas detection device 40 (multi-gas molecule sensor) identifies the types of generated gases and the gas percentages of different gases, and calculates the average gas percentage read by the i-th type of gas detection sensor during the process.

[0059] Based on the proportion of different gases and the pressure difference before and after gas generation, combined with the density of the light liquid in the hydraulic U-tube, the inner diameter of the hydraulic U-tube, the current temperature of the gas generated during the gas measurement process, and the internal environmental volume of the equipment used to detect the gas generation rate, the generation rate of different gases is calculated through a gas production rate calculation model.

[0060] In the gas production rate calculation model, the average rate of the i-th type of gas generated during the reaction process is:

[0061]

[0062] Where ζ is the production rate of each gas during the reaction process, in mol / s; Let p0 be the percentage of gas obtained by the i-th type of gas detection sensor, ρ be the liquid density (g / mL) in the liquid U-tube, g be the acceleration due to gravity (9.8 m / s²), h be the hydraulic pressure difference (m) between the two ends of the liquid U-tube, V0 be the volume of the original gas in the equipment environment used to detect the gas generation rate, r be the inner diameter of the liquid U-tube, R be the standard molar gas mass (8.314 J / mol·K), T be the current temperature during the gas measurement process in Kelvin (K), and Δ t This represents the total time (in seconds) from the start of the reaction to the time of detection.

[0063] Therefore, in this embodiment, the gas generation rate can be quickly and accurately calculated using the calculation model of the main control device and the real-time monitored data.

[0064] Reference Figure 2 As shown, Figure 2 This is a schematic diagram illustrating the structure of an embodiment of the device for detecting gas generation rate provided in this application. The gas washing device 20 includes a gas storage tank 201 and a power unit 202; the gas detection device 40 includes multiple gas molecule sensors 401 and a pressure sensing device 402. In one embodiment, the structure of the gas-generating reaction device is a fermentation tank structure, which can be understood as a bio-fermentation tank 50. The bio-fermentation tank 50 contains, but is not limited to, microorganisms and culture media, and generates gas through fermentation reaction of microorganisms and culture media. Further, the number of bio-fermentation tanks 50 is at least one, and in the case of multiple bio-fermentation tanks 50, the multiple bio-fermentation tanks 50 are connected in series through gas pipelines, and the inlet and outlet of the multiple bio-fermentation tanks 50 are equipped with solenoid valves. To illustrate the embodiments of this application in detail, this application embodiment uses one bio-fermentation tank 50 as an example. Further, a temperature detection device 30 is installed at the outlet of the bio-fermentation tank 50. The temperature detection device 30 can be understood as a temperature sensor, which can detect the current temperature of the generated gas in real time.

[0065] Furthermore, the gas storage tank 201, pressure sensing device 402, bio-fermentation tank 50, and multi-gas molecule sensor 401 are connected by gas pipelines, and multiple solenoid valves are installed at the inlet and outlet of the gas pipelines. The outlet of the gas storage tank 201 is connected to the inlet of the pressure sensing device 402; the outlet of the pressure sensing device 402 is connected to the inlet of the bio-fermentation tank 50; and the outlet of the bio-fermentation tank 50 is connected to the inlet of the multi-gas molecule sensor 401. In one embodiment, taking the bio-fermentation tank 50 as a reference, the solenoid valve between the air inlet of the bio-fermentation tank 50 and the air outlet of the pressure sensing device 402 is defined as the first solenoid valve 60; the two solenoid valves between the air outlet of the bio-fermentation tank 50 and the air inlet of the multi-gas molecule sensor 401 are defined as the second solenoid valve 70 and the third solenoid valve 80; the solenoid valve at the end of the air outlet of the bio-fermentation tank 50 is defined as the fourth solenoid valve 90; and the solenoid valve between the air outlet of the gas storage tank 201 and the air inlet of the pressure sensing device 402 is defined as the fifth solenoid valve 100. Therefore, it can be understood that the main control device 10 controls the opening or closing of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, the fourth solenoid valve 90, and the fifth solenoid valve 100, and controls the connection or disconnection between the gas storage tank 201, the pressure sensing device 402, the bio-fermentation tank 50, and the multi-gas molecule sensor 401.

[0066] It should be further noted that one form of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, the fourth solenoid valve 90, and the fifth solenoid valve 100 may be a DF2-3-B type double-way solenoid valve.

[0067] In one embodiment, the multiple gas molecule sensor 401 includes a gas concentration detection sensor, which can be understood as having a gas concentration detection sensor placed in series on the gas channel between the two ends of the multiple gas molecule sensor 401. Further, one end of the multiple gas molecule sensor 401 is connected to the internal environment of the device detecting the gas generation rate, and the other end is connected to the external atmospheric environment. Further, a one-way valve 110 is also installed at the end connected to the atmospheric environment, which can be understood as the multiple gas molecule sensor 401 having a unidirectional gas output direction, that is, gas can only exit from the inside of the device detecting the gas generation rate to the external atmospheric environment, and the external atmospheric environment cannot enter the inside of the device detecting the gas generation rate. Specifically, the multiple gas molecule sensor 401 identifies the types of gases generated by the device detecting the gas generation rate during the fermentation reaction and detects the proportion of different gases.

[0068] In one embodiment, the pressure sensing device 402 contains a preset liquid, which is a light liquid such as kerosene or vegetable oil. Further, the pressure sensing device 402 has a U-shaped tube structure, thus it can be understood as a hydraulic U-tube. Further, one end of the hydraulic U-tube is connected to the external atmospheric environment, i.e., it is in communication with the outside air; the other end of the hydraulic U-tube is connected to the internal environment of the device detecting the gas generation rate. Further, a liquid level sensor is also installed at the end of the hydraulic U-tube connected to the atmosphere. Specifically, the hydraulic U-tube obtains the liquid level difference between the end connected to the external atmospheric environment and the end connected to the internal environment through the liquid level sensor, and obtains the pressure change difference before and after gas generation through the liquid level difference at both ends. Further, the main control device calculates the total gas molar mass using a calculation formula combined with the pressure change difference, and detects the total gas molar mass generated during the fermentation reaction of the device detecting the gas generation rate.

[0069] In one embodiment, the main control device 10 can be understood as a microcontroller main control device, and one manifestation of the main control device 10 can be a microcontroller main control device based on Arduino Mega 2560. The main control device 10 includes a solenoid valve control port, a stepper motor control port, and a digital communication protocol port. Further, the main control device 10 controls the opening or closing of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, the fourth solenoid valve 90, and the fifth solenoid valve 100 through the solenoid valve control port. Further, the main control device 10 controls the stepper motor of the power device 202 to drive it through the stepper motor control port.

[0070] Furthermore, the main control device 10 acquires the current temperature of the gas generated during the gas measurement process, the type of gas generated, the proportion of different gases, and the pressure difference before and after gas generation through the digital communication protocol port, in order to calculate the generation rate of different gases. It should be noted that when the detected gas is hydrogen or carbon dioxide, and the inert gas is nitrogen, the proportion of each gas is converted into a digital signal by a gas concentration-digital signal transmitter based on the RS485 protocol.

[0071] Furthermore, the main control unit 10 updates the current gas generation rate in real time and plots a visualization curve based on recorded historical data. Alternatively, it can upload the data to other analysis centers for data analysis to meet other production requirements.

[0072] In one embodiment, the gas storage tank 201 stores inert gas and is configured as a piston-like structure, similar to a syringe structure. The inert gas refers to Group 18 elements on the periodic table (IUPAC new definition, i.e., the original Group 0).

[0073] Furthermore, the power unit 202 pushes the piston in the gas storage tank 201 to drive the inert gas to clean the gas pipeline and discharge the gas generated during fermentation. In other words, the main control unit 10 controls the power unit 202 to drive the inert gas in the gas storage tank 201 to clean the gas pipeline and discharge the gas generated during fermentation. (Refer to...) Figure 3 , Figure 3 This is a structural schematic diagram of the pre-reaction pipeline cleaning process provided in this application. Specifically, the main control device 10 controls the opening of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, and the fifth solenoid valve 100, and controls the closing of the fourth solenoid valve 90. At the same time, it controls the stepper motor of the power device 202 to push the piston of the gas storage tank 201 towards the gas outlet. At this time, the original gas in the internal environment of the device that detects the gas generation rate will be gradually replaced by inert gas. Among them, the device 120 is the push screw of the gas washing device 20.

[0074] Furthermore, the internal volume of the gas storage tank 201 is a preset multiple of the internal volume of the device for detecting the gas generation rate. The preset multiple is set according to the actual situation. Typically, the internal volume of the gas storage tank 201 is more than twice the internal volume of the device for detecting the gas generation rate, with the aim of completely discharging the gas from the device for detecting the gas generation rate.

[0075] Furthermore, the power unit 202 includes a stepper motor, which is connected to the main control unit 10. This means that the main control unit 10 controls the power unit 202 through the stepper motor. The stepper motor can be a 28BYJ48 micro motor.

[0076] Specifically, the main control device 10 controls the power device 202 to drive the stepper motor. The power device 202 drives the piston in the gas storage tank 201 to continuously pressurize the gas at the outlet of the gas storage tank 201, thereby injecting the inert gas in the gas storage tank 201 into the device for detecting the gas generation rate. The inert gas cleans the residual gas before the reaction of the device for detecting the gas generation rate and discharges the generated gas after the reaction of the device for detecting the gas generation rate. That is, it cleans the gas pipeline and discharges the gas generated during the fermentation process.

[0077] Furthermore, the specific analysis of the hydraulic differential generated by the hydraulic U-tube is as follows: (Refer to...) Figure 4 , Figure 4 This is a schematic diagram of the hydraulic pressure difference generated during the detection process of the device for detecting gas generation rate provided in this application. Since the hydraulic pressure difference generation process occurs during the fermentation reaction in fermenter 50, the main control device 10 controls the first solenoid valve 60 to open and controls the second solenoid valve 70, the third solenoid valve 80, the fourth solenoid valve 90, and the fifth solenoid valve 100 to close. The third solenoid valve 80 and the fourth solenoid valve 90... Figure 4 Omitted in .

[0078] During the fermentation process, the gas generated by the microbial reaction pushes the liquid in the pressure sensing device 402 (liquid U-tube) upward, creating a hydraulic pressure difference h. At this time, the volume and pressure of the gas in the device that detects the gas generation rate change. The liquid level sensor 130, located at the end of the liquid U-tube that connects to the atmospheric environment, can read the hydraulic pressure difference between the two ends of the liquid U-tube. The liquid level sensor 130 can be an optical liquid level sensor, which means that the hydraulic pressure difference between the two ends of the liquid U-tube is determined by the optical liquid level sensor.

[0079] Furthermore, referring to Figure 5 As shown, Figure 5 This is a structural schematic diagram of the post-reaction pipeline cleaning process provided in this application. Specifically, the main control device 10 controls the opening of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, and the fifth solenoid valve 100, and controls the closing of the fourth solenoid valve 90, while simultaneously controlling the piston in the gas storage tank 201 to move towards the gas outlet.

[0080] The bioreactor 50 requires temperature during the fermentation process; therefore, a temperature control device needs to be connected to the bioreactor 50. The temperature control device provides the necessary temperature for the fermentation process in the bioreactor 50.

[0081] In one embodiment, the specific process for detecting the gas generation rate is as follows:

[0082] The gas storage tank 201 is filled with inert gas (such as helium, nitrogen, etc.), and the bio-fermentation tank 50 contains microorganisms. Before the microbial fermentation reaction begins, the original gas in the internal environment of the device for detecting the gas generation rate needs to be purged. Specifically, the main control device 10 controls the opening of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, and the fifth solenoid valve 100, and controls the closing of the fourth solenoid valve 90. At the same time, it controls the stepper motor of the power device 202 to push the piston of the gas storage tank 201 towards the gas outlet. At this time, the original gas in the internal environment of the device for detecting the gas generation rate will be gradually replaced by inert gas.

[0083] During this process, the main control device 10 continuously reads the types of gases generated by the various gas molecule sensors 401 and the proportion of different gases, such as... Figure 5 As shown, the multiple gas molecule sensors 401 include: a gas sensor 4011 for inert gas a, a gas sensor 4012 for other gases b, and a gas sensor 4013 for other gases c. Therefore, the gas proportion includes the proportion of inert gas. Thus, the inert gas proportion is compared with a preset gas proportion, which is set according to actual conditions.

[0084] If the proportion of inert gas is determined to be higher than the preset gas proportion, then the remaining raw gas in the device for detecting the gas generation rate meets the requirements. If the proportion of inert gas is determined to be lower than the preset gas proportion, then the remaining raw gas in the device for detecting the gas generation rate does not meet the requirements, and inert gas needs to be continuously injected into the internal environment of the device for detecting the gas generation rate until the proportion of inert gas is higher than the preset gas proportion.

[0085] Furthermore, if the proportion of inert gas is determined to be higher than the preset gas proportion, the main control device 10 controls the second solenoid valve 70, the third solenoid valve 80, the fourth solenoid valve 90, and the fifth solenoid valve 100 to close, and controls the first solenoid valve 60 to open, while recording this moment as the first time t0. During the reaction, the gas generated by the microbial reaction will push the liquid in the pressure sensing device 402 (liquid U-tube) to rise, generating a hydraulic pressure difference. At this time, the volume and pressure of the gas in the device that detects the gas generation rate change. The liquid level sensor 130, placed at the end of the liquid U-tube that connects to the atmospheric environment, can read the hydraulic pressure difference between the two ends of the liquid U-tube. The liquid level sensor 130 can be an optical liquid level sensor, which can be understood as: determining the hydraulic pressure difference between the two ends of the liquid U-tube through the optical liquid level sensor, thereby determining the pressure change difference before and after gas generation.

[0086] Furthermore, the total molar mass M of all gases in the device for detecting gas generation rate is calculated based on the hydraulic pressure difference between the two ends of the liquid U-tube. The total molar mass M is:

[0087]

[0088] Where p0 is atmospheric pressure, ρ is the liquid density (g / mL) in the liquid U-tube, g is the gravitational acceleration (9.8 m / s²), h is the hydraulic pressure difference (m) between the two ends of the liquid U-tube, V0 is the volume of the original gas in the environment inside the device for detecting the gas generation rate, r is the inner diameter of the liquid U-tube, R is the standard molar gas mass (8.314 J / mol·K), and T is the current temperature during the gas measurement process, in Kelvin (K).

[0089] Furthermore, the total gas molar mass is compared with the preset molar mass to obtain the comparison result, wherein the preset molar mass is set according to the actual situation.

[0090] If the comparison result shows that the total gas molar mass is greater than the preset molar mass, meaning the hydraulic pressure difference across the liquid U-tube has reached the required level, then the device for detecting the gas generation rate has collected enough gas for analysis, and this moment is recorded as the second time t1. If the comparison result shows that the total gas molar mass is less than or equal to the preset molar mass, meaning the hydraulic pressure difference across the liquid U-tube has not reached the required level, then the device for detecting the gas generation rate has not collected enough gas to meet the analytical requirements. Therefore, the reaction time needs to be extended until the total gas molar mass is greater than the preset molar mass.

[0091] Furthermore, if the total number of gas moles is determined to be greater than the preset number of moles, that is, the hydraulic pressure difference between the two ends of the liquid U-tube reaches the required hydraulic pressure difference, then the various generated gases in the reaction process are discharged. Therefore, it is necessary to re-inject inert gas into the device for detecting the gas generation rate to detect the production rate of various gases. Specifically, the main control device 10 controls the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, and the fifth solenoid valve 100 to open, and controls the fourth solenoid valve 90 to close. At the same time, it controls the stepper motor of the power device 202 to push the piston of the gas storage tank 201 towards the gas outlet. At this time, the gas generated in the reaction in the device for detecting the gas generation rate will be gradually replaced by inert gas.

[0092] During the re-injection of inert gas, the main control device 10 continuously reads the types of gases identified and generated by each gas concentration detection sensor in the multi-gas molecule sensor 401, as well as the proportion of different gases, and calculates the average gas proportion read by the i-th type of gas detection sensor during this process. Further combining the density of the light liquid in the hydraulic U-tube, the inner diameter of the hydraulic U-tube, the current temperature of the gas generated during the gas measurement process, and the internal volume of the equipment for detecting the gas generation rate, the generation rate of different gases is calculated using a gas production rate calculation model. In the gas production rate calculation model, the average rate of the i-th type of gas generated during the microbial reaction is:

[0093]

[0094] Where ζ is the production rate of each gas during the reaction process, in mol / s; Let p0 be the percentage of gas obtained by the i-th type of gas detection sensor, ρ be the liquid density (g / mL) in the liquid U-tube, g be the acceleration due to gravity (9.8 m / s²), h be the hydraulic pressure difference (m) between the two ends of the liquid U-tube, V0 be the volume of the original gas in the environment inside the device that detects the gas generation rate, r be the inner diameter of the liquid U-tube, R be the standard molar gas mass (8.314 J / mol·K), T be the current temperature during the gas measurement process in Kelvin (K), and Δ t Δ represents the total time elapsed from the start of the reaction to the time of detection. t = Second time t1 - First time t0.

[0095] Furthermore, the main control unit 10 updates the current gas generation rate in real time and plots a visualization curve based on recorded historical data. Alternatively, it can upload the data to other analysis centers for data analysis to meet other production requirements.

[0096] The device for detecting gas generation rate provided in this application improves the accuracy of gas generation rate detection by using a main control unit to precisely control the solenoid valves of the reaction device before, during, and after the reaction, and by using multiple gas molecule sensors to accurately read the generation rates of various gases during the reaction. Furthermore, the device for detecting gas generation rate provided in this application is simple to assemble and inexpensive, thus reducing the cost of gas generation rate detection.

[0097] Reference Figure 6 , Figure 6 This is a flowchart illustrating the method for detecting gas generation rate provided in this application. The gas generation rate detection method provided in this application is applied to the equipment for detecting gas generation rate, and includes:

[0098] Step S10: Control the gas scrubbing device to pressurize inert gas into the gas pipeline to clean the internal environment of the device for detecting the gas generation rate;

[0099] Step S20: When the rate of gas generation is measured, the solenoid valve is switched on and off and the gas detection device is controlled to identify the types of gases generated during fermentation, as well as to detect the proportion of different gases and the pressure difference before and after gas generation.

[0100] Step S30: Calculate the generation rate of different gases based on the gas proportions of different gases and the pressure difference before and after gas generation, combined with the current temperature of the generated gas.

[0101] It should be noted that the main control device is defined as the main control device 10. When detecting the production speed of various gases, it is necessary to send detection commands to the main control device 10.

[0102] It should be further noted that the main control device 10 is connected to the gas scrubbing device 20, multiple solenoid valves, temperature detection device 30, and gas detection device 40. The gas scrubbing device 20 includes a gas storage tank 201 and a power unit 202; the gas detection device 40 includes multiple gas molecule sensors 401 and a pressure sensing device 402.

[0103] The gas scrubbing device 20 and the gas detection device 40 are connected to the bio-fermentation tank 50 via gas pipelines. Multiple solenoid valves are installed at the inlet and outlet of the gas pipelines. The temperature detection device 30 is installed at the outlet of the bio-fermentation tank 50.

[0104] Furthermore, the gas storage tank 201, pressure sensing device 402, bio-fermentation tank 50 and multiple gas molecule sensors 401 are connected by gas pipelines, and multiple solenoid valves are installed at the gas inlet and outlet of the gas pipelines.

[0105] The outlet of the gas storage tank 201 is connected to the inlet of the pressure sensing device 402; the outlet of the pressure sensing device 402 is connected to the inlet of the bio-fermentation tank 50; and the outlet of the bio-fermentation tank 50 is connected to the inlet of the multi-gas molecule sensor 401. In one embodiment, taking the bio-fermentation tank 50 as a reference, the solenoid valve between the inlet of the bio-fermentation tank 50 and the outlet of the pressure sensing device 402 is defined as the first solenoid valve 60; the two solenoid valves between the outlet of the bio-fermentation tank 50 and the inlet of the multi-gas molecule sensor 401 are defined as the second solenoid valve 70 and the third solenoid valve 80; the solenoid valve at the end of the outlet of the bio-fermentation tank 50 is defined as the fourth solenoid valve 90; and the solenoid valve between the outlet of the gas storage tank 201 and the inlet of the pressure sensing device 402 is defined as the fifth solenoid valve 100.

[0106] Specifically, the gas storage tank 201 is filled with inert gas, and the bio-fermentation tank 50 contains microorganisms. Before the microbial fermentation reaction begins, the original gas in the internal environment of the device for detecting the gas generation rate needs to be discharged. Specifically, after receiving the detection command, the main control device 10 controls the opening of the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, and the fifth solenoid valve 100, and controls the closing of the fourth solenoid valve 90. At the same time, the stepper motor of the power device 202 is controlled to push the piston of the gas storage tank 201 towards the gas outlet. At this time, the original gas in the internal environment of the device for detecting the gas generation rate will be gradually replaced by inert gas.

[0107] During this process, the main control device 10 continuously reads the types of gases generated by the multi-gas molecule sensor 401 and the proportion of different gases. Among the multi-gas molecule sensors 401 is an inert gas concentration detection sensor, so the gas proportion includes the inert gas proportion. Therefore, the inert gas proportion is compared with a preset gas proportion, which is set according to the actual situation.

[0108] If the proportion of inert gas is determined to be higher than the preset gas proportion, then the remaining raw gas in the device for detecting the gas generation rate meets the requirements. If the proportion of inert gas is determined to be lower than the preset gas proportion, then the remaining raw gas in the device for detecting the gas generation rate does not meet the requirements, and inert gas needs to be continuously injected into the internal environment of the device for detecting the gas generation rate until the proportion of inert gas is higher than the preset gas proportion.

[0109] Furthermore, if the proportion of inert gas is determined to be higher than the preset gas proportion, the main control device 10 begins measuring the gas generation rate. Specifically, the main control device 10 controls the second solenoid valve 70, the third solenoid valve 80, the fourth solenoid valve 90, and the fifth solenoid valve 100 to close, and controls the first solenoid valve 60 to open, while recording this moment as the first time t0. During the reaction, the gas generated by the microbial reaction will push the liquid in the pressure sensing device 402 (liquid U-tube) to rise, generating a hydraulic pressure difference. At this time, the volume and pressure of the gas in the device for detecting the gas generation rate change. The liquid level sensor 130, placed at the end of the liquid U-tube connected to the atmospheric environment, can read the hydraulic pressure difference between the two ends of the liquid U-tube. The liquid level sensor 130 can be an optical liquid level sensor, which means that the hydraulic pressure difference between the two ends of the liquid U-tube is determined by the optical liquid level sensor, thereby determining the pressure change difference before and after gas generation.

[0110] Furthermore, the total gas molar mass M of all gases in the device for detecting gas generation rate is calculated based on the hydraulic pressure difference between the two ends of the liquid U-tube.

[0111] Furthermore, the total gas molar mass is compared with the preset molar mass to obtain the comparison result, wherein the preset molar mass is set according to the actual situation.

[0112] If the comparison result shows that the total gas molar mass is greater than the preset molar mass, meaning the hydraulic pressure difference across the liquid U-tube has reached the required level, then the device for detecting the gas generation rate has collected enough gas for analysis, and this moment is recorded as the second time t1. If the comparison result shows that the total gas molar mass is less than or equal to the preset molar mass, meaning the hydraulic pressure difference across the liquid U-tube has not reached the required level, then the device for detecting the gas generation rate has not collected enough gas to meet the analytical requirements. Therefore, the reaction time needs to be extended until the total gas molar mass is greater than the preset molar mass.

[0113] Furthermore, if the total number of gas moles is determined to be greater than the preset number of moles, that is, the hydraulic pressure difference between the two ends of the liquid U-tube reaches the required hydraulic pressure difference, then the various generated gases in the reaction process are discharged. Therefore, it is necessary to re-inject inert gas into the device for detecting the gas generation rate to detect the production rate of various gases. Specifically, the main control device 10 controls the first solenoid valve 60, the second solenoid valve 70, the third solenoid valve 80, and the fifth solenoid valve 100 to open, and controls the fourth solenoid valve 90 to close. At the same time, it controls the stepper motor of the power device 202 to push the piston of the gas storage tank 201 towards the gas outlet. At this time, the gas generated in the reaction in the device for detecting the gas generation rate will be gradually replaced by inert gas.

[0114] During the re-injection of inert gas, the main control device 10 continuously reads the types of gases identified and generated by each gas concentration detection sensor in the multi-gas molecule sensor 401, as well as the proportion of different gases, and calculates the average gas proportion read by the i-th type of gas detection sensor during this process. Further combining the density of the light liquid in the hydraulic U-tube, the inner diameter of the hydraulic U-tube, the current temperature of the gas generated during the gas measurement process, and the internal volume of the equipment for detecting the gas generation rate, the generation rate of different gases is calculated using a gas production rate calculation model. In the gas production rate calculation model, the average rate of the i-th type of gas generated during the microbial reaction is:

[0115]

[0116] Where ζ is the production rate of each gas during the reaction process, in mol / s; Let p0 be the percentage of gas obtained by the i-th type of gas detection sensor, ρ be the liquid density (g / mL) in the liquid U-tube, g be the acceleration due to gravity (9.8 m / s²), h be the hydraulic pressure difference (m) between the two ends of the liquid U-tube, V0 be the volume of the original gas in the environment inside the device that detects the gas generation rate, r be the inner diameter of the liquid U-tube, R be the standard molar gas mass (8.314 J / mol·K), T be the current temperature during the gas measurement process in Kelvin (K), and Δ t Let Δ be the total time elapsed from the start of the reaction to the time of detection. t = Second time t1 - First time t0.

[0117] Furthermore, the main control unit 10 updates the current gas generation rate in real time and plots a visualization curve based on recorded historical data. Alternatively, it can upload the data to other analysis centers for data analysis to meet other production requirements.

[0118] The gas generation rate detection method provided in this application improves the accuracy of gas generation rate detection by using a main control device to precisely control the solenoid valves of the reaction device before, during, and after the reaction, and by using multiple gas molecule sensors to accurately read the generation rates of various gases during the reaction. Furthermore, the device for detecting gas generation rate provided in this application is simple to assemble and inexpensive, thus reducing the cost of gas generation rate detection.

[0119] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A device for detecting the rate of gas generation, characterized in that, It includes a main control unit, a gas scrubbing unit, a gas detection unit, and a temperature detection unit; The gas scrubbing device is used to clean gas pipelines and discharge generated gas. The temperature detection device is used to detect the current temperature of the generated gas; The gas detection device is used to identify the type of gas generated, and to detect the proportion of different gases and the pressure difference before and after gas generation. The main control device is used to control the gas scrubbing device, the gas detection device, and the temperature detection device. The main control device includes a gas production rate calculation model, which, within a measurement time, calculates the generation rate of different gases based on the gas percentage detected by the gas detection device, the pressure difference before and after gas generation, and the current temperature of the generated gas detected by the temperature detection device. This includes: The gas scrubbing device is controlled to inject inert gas into the gas pipeline. If the proportion of inert gas is higher than a preset gas proportion, it is determined that the remaining raw gas in the gas pipeline meets the requirements. If the gas generated by the microbial reaction enters the gas pipeline and the total molar mass of all gases in the gas pipeline is greater than a preset molar mass, it is determined that the gas pipeline has collected enough gas for analysis. The current temperature is used in the calculation of the total molar mass. The gas scrubbing device is then controlled to inject inert gas into the gas pipeline again. Based on the gas proportion detected by the gas detection device and the pressure difference before and after gas generation, combined with the current temperature of the generated gas detected by the temperature detection device, the generation rate of different gases is calculated. The gas scrubbing device includes a gas storage tank and a power unit; the gas storage tank stores inert gas; the main control unit controls the power unit to drive the inert gas in the gas storage tank to clean the gas pipeline and discharge the gas generated during the fermentation process. The gas detection device includes multiple gas molecule sensors and a pressure sensing device, and the reaction device for generating gas is a bio-fermentation tank; the gas outlet of the gas storage tank is connected to the gas inlet of the pressure sensing device; the gas outlet of the pressure sensing device is connected to the gas inlet of the bio-fermentation tank; and the gas outlet of the bio-fermentation tank is connected to the gas inlet of the multiple gas molecule sensors. The pressure sensing device includes a hydraulic U-tube and a liquid level sensor placed in the hydraulic U-tube and connected to the atmosphere at the end. The hydraulic U-tube contains a light liquid, with one end connected to the external atmospheric environment and the other end connected to the internal environment of the device for detecting the gas generation rate; the liquid level sensor detects the liquid level difference before and after gas generation to obtain the pressure change difference. The number of bio-fermentation tanks is one, which is connected to the gas scrubbing device and the gas detection device through a gas pipeline. Multiple solenoid valves are installed at the inlet and outlet of the gas pipeline. The gas scrubbing device and the pressure sensing device are located on the inlet side of the bio-fermentation tank. The multiple gas molecule sensors are located on the outlet side of the bio-fermentation tank.

2. The device for detecting gas generation rate according to claim 1, characterized in that, in, The multiple gas molecule sensors are used to identify the types of generated gases and detect the proportion of different gases. The multiple gas molecule sensors are placed in series on the gas pipeline, with one end connected to the internal environment of the device for detecting the gas generation rate and the other end connected to the external atmospheric environment. The pressure sensing device is used to detect the pressure difference before and after gas generation.

3. The apparatus for detecting gas generation rate according to any one of claims 1 to 2, characterized in that, The main control device obtains the current temperature of the gas generated during the gas measurement process, the type of gas generated, the proportion of different gases, and the pressure change difference before and after gas generation through a digital communication protocol port, so as to calculate the generation rate of different gases; the main control device includes a single-chip microcomputer main control device.

4. The device for detecting gas generation rate according to claim 1, characterized in that, The gas storage tank is configured with a piston-driven structure. The piston in the gas storage tank is driven by the power device to push the inert gas to clean the gas pipeline. The power device includes a stepper motor.

5. The device for detecting gas generation rate according to claim 4, characterized in that, The number of the bio-fermentation tanks is at least one; the inlet and outlet of the bio-fermentation tanks are equipped with solenoid valves; the temperature detection device is located at the outlet of the bio-fermentation tanks.

6. A method for detecting gas generation rate, based on the apparatus for detecting gas generation rate according to any one of claims 1 to 5, characterized in that, include: The gas scrubbing device is controlled to pressurize inert gas into the gas pipeline to clean the internal environment of the device for detecting the gas generation rate. When the rate of gas generation is measured, the solenoid valve is switched on and off and the gas detection device is controlled to identify the types of gases generated during fermentation, as well as to detect the proportion of different gases and the pressure difference before and after gas generation. The generation rate of different gases is calculated based on the proportion of different gases, the pressure difference before and after gas generation, and the current temperature of the generated gases.

7. The method for detecting gas generation rate according to claim 6, characterized in that, The calculation of the generation rate of different gases based on their proportions, pressure differences before and after gas generation, and the current temperature of the generated gases includes: Based on the proportion of different gases and the pressure difference before and after gas generation, combined with the density of the light liquid in the hydraulic U-tube, the inner diameter of the hydraulic U-tube, the current temperature, and the internal environmental volume of the device for detecting gas generation rate, the generation rate of different gases is calculated using a gas production rate calculation model.

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