Method for using a plant growth regulator containing calcium chelate and application thereof

Abstract

The present application relates to a kind of plant growth regulator containing calcium cyclodextrin and its application, the effective active ingredient of the plant growth regulator protected by the present application is choline chloride and calcium cyclodextrin, wherein the weight ratio of choline chloride and calcium cyclodextrin is 9:1.Experiments show that the plant growth regulator protected by the present application has good synergistic effect for regulating ginger growth, in ginger three fork period (3 seedling period), according to the effective component dosage 112.5-300 g / hm2, even spraying, 1 time of application, can significantly reduce the plant height of ginger, increase ginger stem thickness, can increase branch and leaf number, significantly increase yield.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a method of using and application of a plant growth regulator containing calcium cyclohexane. The plant growth regulator is composed of choline chloride, calcium cyclohexane, and adjuvants. Background Technology

[0002] Choline chloride, with the molecular formula C5H4O 14 ClNO, with a molecular weight of 139.625, is mainly used as a feed additive. In the field of pesticides, it can be absorbed through the stems, leaves, and roots of plants and then quickly transported to the site of action. Its physiological effects can inhibit light absorption in C3 plants, promote root development, and allow photosynthetic products to accumulate in tubers and roots as much as possible, thereby increasing yield and improving quality.

[0003] Calcium cyclohexane was first used in Japan to prevent lodging in rice, and later in Europe and America for controlling excessive growth in lawns and fruit trees, as well as for preventing fire blight in fruit trees. Calcium cyclohexane is a calcium salt of cyclohexanecarboxylate, and the active ingredient is cyclohexane. When sprayed onto plants, calcium cyclohexane is rapidly absorbed by the leaf cells of the crop. Since gibberellins are synthesized in the leaves, it can directly target the plant, thus exhibiting high activity. Furthermore, calcium cyclohexane has a very short half-life; in soils rich in microorganisms, the half-life does not exceed 24 hours. Moreover, the final metabolic products of calcium cyclohexane are carbon dioxide and water, making it a low-toxicity, residue-free, and environmentally friendly product.

[0004] The applicant discovered through experiments that combining choline chloride, which has a different mechanism of action, with calcium cyclohexane has a good effect on increasing ginger yield and quality, and is safe for ginger without causing phytotoxicity. Summary of the Invention

[0005] The purpose of this invention is to provide a method and application of using a plant growth regulator containing calcium cyclohexane in regulating ginger growth, thereby further increasing ginger yield and improving the economic benefits of crops.

[0006] The technical solution of this invention is:

[0007] A method for using a plant growth regulator containing calcium cyclohexane and its application, characterized in that: the effective active ingredients of the plant growth regulator are choline chloride and calcium cyclohexane, wherein the weight ratio of choline chloride to calcium cyclohexane is 9:1.

[0008] Furthermore, the total weight of choline chloride and calcium cyclohexane in the plant growth regulator disclosed in this invention is 100-600 g / L, preferably 500 g / L.

[0009] Furthermore, the plant growth regulator disclosed in this invention is composed of choline chloride, calcium cyclamate, and adjuvants, and is formulated into a suspension. The suspension comprises the following components and contents: choline chloride 90-540 g / L, calcium cyclamate 10-60 g / L, synergist 10-80 g / L, dispersant 10-100 g / L, wetting agent 10-100 g / L, defoamer 1-20 g / L, thickener 2-20 g / L, antifreeze 0-100 g / L, preservative 0-10 g / L, pH adjuster 0-20 g / L, and water as the balance.

[0010] Furthermore, the dispersant synergist is selected from one of polyethoxy-modified silane and polyethoxy-modified fatty alcohol mixture or alkoxy-modified polytrisiloxane; the dispersant may be selected from one or more of polycarboxylate, lignin sulfonate, and alkyl naphthalene sulfonate; the wetting agent may be selected from one or more of alkyl sulfate, alkyl sulfonate, and naphthalene sulfonate; the antifreeze agent may be selected from one or more of ethylene glycol, propylene glycol, glycerin, urea, and inorganic salts such as sodium chloride; the thickener may be selected from one or more of silica, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; and the defoamer may be selected from one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, C8-10 fatty alcohols, hexanol, butanol, and octanol.

[0011] Furthermore, this invention provides the application of plant growth regulators in regulating ginger growth.

[0012] The application mentioned refers to all or part of P1-P7:

[0013] P1. Increase ginger yield;

[0014] P2, promotes ginger root development;

[0015] P3, promotes plant photosynthesis and / or improves plant photosynthetic efficiency;

[0016] P4. Reduce plant height;

[0017] P5. Increase the thickness of ginger stems;

[0018] P6. Increase ginger-soluble sugars;

[0019] P7. Increase the number of ginger branches.

[0020] Furthermore, the method of use and application are characterized in that: the plant growth regulator is sprayed at the three-forked stage of ginger (seedling stage 3) according to the effective ingredient dosage of 112.5-300 grams / hectare.

[0021] Furthermore, the present invention provides a method for regulating ginger growth: spraying at the three-branch stage (3-seedling stage) of ginger, according to the effective ingredient dosage of 112.5-187.5 g / ha.

[0022] Compared with the prior art, the plant growth regulator composition of the present invention for regulating ginger growth has the following beneficial effects: (1) Compared with single agents, it has a good synergistic effect on promoting root growth of ginger; (2) The formulation is environmentally friendly, safe for the environment, and odorless, making it safe for producers, transporters, and users; (3) It is low in toxicity and highly effective, reducing the amount of pesticide residue on crops and having a short safety interval; (4) It improves ginger quality, increases ginger stem diameter, reduces plant height, enhances crop photosynthesis, increases the number of branches and leaves, and significantly increases yield. Detailed Implementation

[0023] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0024] Application Example 1

[0025] Dispersant, wetting agent, defoamer, thickener (optional), and antifreeze (optional) are mixed evenly by high-speed shearing. Choline chloride and calcium cyclohexane are added, and the mixture is ball-milled in a ball mill for 2-3 hours until all particles are below 5 μm in diameter. The remaining amount is made up with deionized water to obtain the suspension product described in this invention.

[0026] Example 1: 500 g / L choline chloride-calcium cyclohexane suspension

[0027] A 500 g / L choline chloride·calcium cyclate suspension was prepared by adding 459.18 g of 98% choline chloride technical grade, 52.63 g of 95% calcium cyclate technical grade, 30 g of alkoxy-modified polytrisiloxane, 40 g of alkyl naphthalene sulfonate, 25 g of EO-PO block copolymer, 30 g of alkyl glycoside, 30 g of fatty alcohol polyoxyethylene ether, 5 g of silicone defoamer, 15 g of polyvinyl alcohol, 40 g of propylene glycol, 8 g of sodium benzoate, and water to a final volume of 1000 g.

[0028] Example 2: 500 g / L choline chloride-calcium cyclohexane suspension

[0029] A 500 g / L choline chloride-calcium cyclate suspension was prepared by adding 459.18 g of 98% choline chloride technical grade, 52.63 g of 95% calcium cyclate technical grade, 30 g of a mixture of polyethoxylated modified silane and polyethoxylated modified fatty alcohol, 35 g of alkyl naphthalene sulfonate formaldehyde polymer, 30 g of lignin sulfonate, 25 g of alkylphenol polyoxyethylene ether, 40 g of trisiloxane polyoxyethylene ether, 10 g of fatty alcohol, 8 g of xanthate gum, 30 g of ethylene glycol, 8 g of sodium benzoate, and water to a final volume of 1000 g.

[0030] Example 3: 300 g / L choline chloride-calcium cyclohexane suspension

[0031] A 300 g / L choline chloride·calcium cyclate suspension was prepared by adding 275.51 g of 98% choline chloride technical grade, 31.58 g of 95% calcium cyclate technical grade, 30 g of a mixture of polyethoxylated modified silane and polyethoxylated modified fatty alcohol, 50 g of alkyl naphthalene sulfonate formaldehyde polymer, 20 g of polyarylphenol polyoxyethylene ether, 30 g of alkylphenol polyoxyethylene ether, 10 g of fatty acid, 12 g of carboxymethyl cellulose, 45 g of glycerol, 8 g of sodium benzoate, and water to a final volume of 1000 g.

[0032] Application Example 2: Combined Indoor Toxicity Assay of Choline Chloride and Calcium Cyclocycline for Regulating Ginger Growth

[0033] 1. Experimental Objective

[0034] Choline chloride and calcium cyclamate were used as test agents, and ginger at the three-branch stage was used as the test subject. The combined effect of the two agents was determined by foliar spraying in the laboratory. The optimal ratio was screened to determine the formula, which provides a basis for formula screening for the rational mixing of the two agents and the development of effective agents for regulating ginger growth. The test results are reported as follows.

[0035] 2. Test conditions

[0036] 2.1 Test Target

[0037] The ginger variety is Lujiang No. 1, which has grown to the three-branch stage when grown in indoor pots.

[0038] 2.2 Cultivation Conditions

[0039] Ginger was cultivated using the pot method. The plastic pots were 400x500mm in size, with one ginger seedling transplanted into each pot. A two-sided shaping method was used. The cultivation soil was loam with an organic matter content of 1.83%, pH 7.1, available nitrogen 85.2 mg / kg, available phosphorus 38.3 mg / kg, and available potassium 83.0 mg / kg. Water and fertilizer management followed normal field standards. Cultivation conditions were achieved in a research-grade glass greenhouse, with the temperature controlled at 20-30℃, relative humidity at 65%-75%, and a light-to-dark ratio of 14:10. Watering was done quantitatively as needed.

[0040] 2.3 Instruments and Equipment

[0041] MATABI-BERRY5 sprayer, electronic balance, pipette, volumetric flask, beaker, plastic basin (450mm×350mm), artificial climate chamber, etc.

[0042] 3 Experimental Design

[0043] 3.1 Reagents

[0044] 3.1.1 Test reagents

[0045] 98% choline chloride raw material;

[0046] 95% cyclohexane calcium technical grade.

[0047] 3.1.2 Other reagents

[0048] Emulsifier: Tween 80, Tianjin Kaitong Chemical Reagent Co., Ltd.

[0049] 3.2 Test Treatment

[0050] 3.2.1 Preparation of the stock solution

[0051] The test reagent choline chloride was prepared into a stock solution of 2.5 × 10³ mg / L using distilled water and set aside. The stock solution was then diluted with an aqueous solution of 0.1% Tween 80. Calcium cyclamate is sparingly soluble in water and insoluble in most organic solvents. In the laboratory, it was first processed into a 10% suspension using a sand mill and then diluted to a stock solution of 2.5 × 10³ mg / L for later use.

[0052] 3.2.2 Dosage setting

[0053] Based on the individual activity of the two agents and the characteristics of the laboratory test, and on the basis of the preliminary test, the dosage of choline chloride and calcium cyclohexane were set as different combinations, as shown in Table 1, for a total of 35 groups.

[0054] Distilled water containing the same solvent and emulsifier but without any reagents was used as a blank control for in-house bioassays.

[0055] Table 1. Experimental drug dosage design

[0056]

[0057] 3.2.2 Experimental Repetition

[0058] Each treatment had 4 replicates, with 20 pots per replicate and 1 ginger plant per pot.

[0059] 3.3 Processing Method

[0060] The experiment involved two applications of the pesticide. One ginger plant was placed in each pot. The pesticide was applied when the ginger reached the three-branched stage, and a second application was given 12 days later. The pesticide was applied using a sprayer, with the spray applied evenly. A water treatment served as a control.

[0061] 4. Test Methods

[0062] 4.1 Test Basis and Methods

[0063] The combined effects of foliar spraying and mixed formulations in the plant growth promotion / inhibition test were determined in accordance with the "Guidelines for Indoor Bioassay Tests of Pesticides: Plant Growth Regulators" (NY / T 2061.2-2011, NY / T 2061.5-2016).

[0064] 4.2 Chemical treatment

[0065] The experiment involved applying the pesticide once at the three-branching stage of ginger, followed by a second application 12 days later, for a total of two applications. A BERRY5 sprayer was used according to the experimental design, with a constant pressure of 0.3 MPa. Spraying was performed sequentially from low to high concentration, with a control group (no pesticide) as a blank. The optimal concentration was achieved when the pesticide was evenly applied to all leaves without dripping.

[0066] After treatment, the surface of the test material was allowed to air dry naturally before being transferred to an artificial climate chamber for incubation. The incubation chamber temperature was 25–30°C, the light intensity was 14:10, and the humidity was 65%–75%.

[0067] 5. Data Survey and Statistical Analysis

[0068] 5.1 Results, Survey Time and Methods

[0069] Twenty-one days after the second treatment, the absolute value (numerical measurement) survey method was used, selecting 10 pots with uniform growth in each replicate to investigate the plant height of ginger. Fifty days after the second treatment, the absolute value (numerical measurement) survey method was used, selecting 10 pots with uniform growth in each replicate to investigate the number of branches and yield per plant (removing the mother ginger and retaining only the new ginger).

[0070] 5.2 Data Statistical Analysis

[0071] Based on the measurement results, the inhibition rate of plant height, the increase rate of branch number, and the increase rate of single-plant tuber weight of ginger under different treatments were calculated.

[0072] The theoretical inhibition rate (growth rate) E0 (E0=X+YX*Y / 100) of each treatment combination was calculated using the Gowing method, and then compared with the measured inhibition rate (growth rate) (E) to evaluate the combined effect of the two treatments on ginger.

[0073] When the E-E0 value is greater than 10%, it indicates a synergistic effect; when the E-E0 value is less than -10%, it indicates an antagonistic effect; and when the E-E0 value is between -10% and 10%, it indicates an additive effect. The optimal ratio is determined based on factors such as actual efficacy, the characteristics of the two drugs, and the balance of the formulation.

[0074] In the formula, X represents the plant height inhibition rate (branch number growth rate and single-plant tuber weight growth rate) when the dosage of choline chloride is P; Y represents the plant height inhibition rate (branch number growth rate and single-plant tuber weight growth rate) when the dosage of calcium cyclamate is Q. The statistical results are shown in Table 2.

[0075] 6 Results and Analysis

[0076] Table 2. Indoor combined effect determination of choline chloride and calcium cyclophosphamide on ginger (Gowing method)

[0077]

[0078]

[0079] Table 3. Indoor combined effect determination of choline chloride and calcium cyclophosphamide on ginger (Gowing method)

[0080]

[0081]

[0082] As shown in Tables 2 and 3, the combined effects of the experimental agents, evaluated using the Gowing method, revealed that the E-E0 values ​​for each treatment ranged from -10% to 10%, indicating an additive effect. When the E-E0 value was greater than 10%, it indicated a synergistic effect. The E-E0 value for the branching rate increase in the treatment with 270 mg / L choline chloride + 30 mg / L calcium cyclamate (9:1 ratio) was 10.02, greater than 10%, indicating a synergistic effect. The E-E0 value for the single-plant tuber weight increase was 10.15, greater than 10%, also indicating a synergistic effect. This treatment significantly regulated excessive plant growth, increased branching rate, and increased single-plant tuber weight, demonstrating a certain synergistic effect.

[0083] Application Example 3: Field efficacy experiment of 500 g / L choline chloride·calcium cyclamate suspension (Example 1) in regulating ginger growth

[0084] 1. Experimental Objective

[0085] The efficacy of 500 g / L choline chloride-calcium cyclate suspension (Example 1) in regulating ginger growth was evaluated in the field. The experiment screened reasonable field application concentrations, timing and methods to provide a basis for the registration of this product.

[0086] 2 Materials and Methods

[0087] 2.1 Treatment of experimental reagents

[0088] Table 4 Treatment of test reagents

[0089]

[0090] Based on the dosage of 450L of pesticide solution per hectare and the formulation dosage, the pesticide was weighed and placed into centrifuge tubes, which were then labeled. Four tubes were used for each treatment. The dosages were 0.45mL, 0.6mL, 0.75mL, and 1.2mL of the experimental pesticide, and 0.6mL of 60% choline chloride aqueous solution and 2.25g of 5% calcium cyclamate effervescent granules as control pesticides. The pesticide was then poured into 900mL of water, mixed thoroughly, and sprayed onto the corresponding plots of plants.

[0091] 2.2 Experimental subjects and crops

[0092] Experimental subject: Regulated growth.

[0093] Experimental crop: ginger, specifically the Sichuan Zhugen ginger variety.

[0094] 2.3 Experimental site and cultivation conditions

[0095] Test location: Tongliang District, Chongqing.

[0096] 2.4 Processing Methods

[0097] Area of ​​the residential area: 20m2, with 2 rows of protective lines installed.

[0098] Number of repetitions: 4.

[0099] 2.5 Application Time and Method

[0100] The experiment involved one application of the pesticide, which was applied once at the three-forked (3-seedling) stage of ginger.

[0101] 2.6 Survey Methods

[0102] Three and seven days after application, a survey of phytotoxicity (negative impacts) was conducted. The effects of the pesticide on wild organisms and beneficial insects in the experimental area, as well as on other pests and diseases, were recorded. Ginger plants in each pesticide-treated area were visually inspected for symptoms of phytotoxicity such as wilting, leaf drop, deformity, yellowing, and discoloration.

[0103] At the time of ginger harvest (October 20), five ginger plants were randomly selected from each plot to record plant height, stem diameter, and number of branches, and the yield per plant and the yield of the plot were measured. After harvest, on October 21, the quality of ginger, including soluble protein, crude fiber, and soluble sugar, was measured.

[0104] Output: Weighed by platform scale

[0105] Coarse fiber: acid-washing method

[0106] Soluble sugars: anthrone method

[0107] Soluble proteins: Coomassie brilliant blue colorimetric method

[0108] The results are the average values ​​of each treatment. SPSS statistical software was used for analysis, and the yield results were statistically analyzed using Duncan's New Multiple Range Method (DMRT).

[0109] 2.7 Calculation and Analysis Methods

[0110]

[0111] 3 Results and Analysis

[0112] Table 5. Effects of 500 g / L choline chloride·calcium cyclamate suspension on ginger growth traits

[0113]

[0114] Note: The values ​​listed in the table are the averages of four replicates. Lowercase letters following the values ​​in the same column indicate the variance at the p-value. 0.05 Significance at the level, with uppercase letters indicating significance at point P. 0.01 Significance of differences at different levels. The same applies to the following table.

[0115] Table 6. Effects of 500 g / L choline chloride·calcium cyclamate suspension on ginger yield increase

[0116]

[0117] Table 7. Effects of 500 g / L choline chloride·calcium cyclamate suspension on ginger quality

[0118]

[0119] The experimental results showed that, under the experimental conditions, the effective ingredient dosage of the test agent 500 g / L choline chloride·calcium cyclate suspension at a dose of 112.5-300 g / ha, applied once at the three-forked (3 seedling) stage of ginger, had a regulatory effect on ginger growth. In the field, the main effects were increased stem diameter and number of branches, which could also significantly increase ginger yield and improve ginger quality. There were no adverse effects on other non-target organisms.

[0120] Application Example 4: Field efficacy experiment of 500 g / L choline chloride·calcium cyclamate suspension (Example 2) in regulating ginger growth

[0121] For specific experimental objectives, experimental basis, experimental reagents and dosages, investigation methods and calculation methods, please refer to Application Example 3.

[0122] 1. Experimental crop:

[0123] The ginger variety is white ginger;

[0124] Experimental location: Fengdu County, Chongqing

[0125] 2. Application time and frequency

[0126] Apply the herbicide once at the three-forked (3 seedlings) stage of ginger (June 21, 2021).

[0127] 3. Survey time and number of times

[0128] At harvest time (October 6), the height of ginger plants, stem diameter, and number of branches were investigated, and the yield per plant and the yield per plot were measured to calculate the yield increase rate.

[0129] The quality of ginger, including soluble protein content, soluble sugar content, and crude fiber, was determined on October 7th and 8th.

[0130] 4 Experimental Results

[0131] Table 8 Effects of 500 g / L choline chloride·calcium cyclamate suspension on ginger agronomic traits

[0132]

[0133] Table 9. Effects of 500 g / L choline chloride·calcium cyclamate suspension on ginger yield increase

[0134]

[0135] Table 10 Effect of 500 g / L choline chloride·calcium cyclamate suspension on ginger quality

[0136]

[0137] The experimental results showed that, under the experimental conditions, when the effective ingredient dosage of the 500 g / L choline chloride-calcium cyclate suspension was 112.5-300 g / ha (i.e., the dosage of the formulation was 15-40 ml / mu), the effect of spraying once at the three-forked (3 seedling) stage of ginger was significant, as evidenced by increased ginger plant height, stem diameter, number of branches per plant, yield per plant, and significantly increased plot yield.

[0138] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. An application of a plant growth regulator containing calcium cyclohexane for regulating ginger growth, characterized in that: The application described has all or some of the following functions: P1. Increase ginger yield; P2, promotes ginger root development; P3, promotes ginger photosynthesis and / or improves plant photosynthetic efficiency; P4. Reduce ginger plant height; P5. Increase the thickness of ginger stems; P6. Increase ginger-soluble sugars; P7. Increase the number of ginger branches; The plant growth regulator contains the following components and their contents: A 500 g / L choline chloride·calcium cyclate suspension was prepared by adding 459.18 g of 98% choline chloride technical grade, 52.63 g of 95% calcium cyclate technical grade, 30 g of alkoxy-modified polytrisiloxane, 40 g of alkyl naphthalene sulfonate, 25 g of EO-PO block copolymer, 30 g of alkyl glycoside, 30 g of fatty alcohol polyoxyethylene ether, 5 g of silicone defoamer, 15 g of polyvinyl alcohol, 40 g of propylene glycol, 8 g of sodium benzoate, and water to a final volume of 1000 g.

2. An application of a plant growth regulator containing calcium cyclohexane for regulating ginger growth, characterized in that: The application described has all or some of the following functions: P1. Increase ginger yield; P2, promotes ginger root development; P3, promotes ginger photosynthesis and / or improves plant photosynthetic efficiency; P4. Reduce ginger plant height; P5. Increase the thickness of ginger stems; P6. Increase ginger-soluble sugars; P7. Increase the number of ginger branches; The plant growth regulator contains the following components and their contents: A 500 g / L choline chloride·calcium cyclate suspension was prepared by adding 459.18 g of 98% choline chloride technical grade, 52.63 g of 95% calcium cyclate technical grade, 30 g of a mixture of polyethoxylated modified silane and polyethoxylated modified fatty alcohol, 35 g of alkyl naphthalene sulfonate formaldehyde polymer, 30 g of lignin sulfonate, 25 g of alkylphenol polyoxyethylene ether, 40 g of trisiloxane polyoxyethylene ether, 10 g of fatty alcohol, 8 g of xanthate gum, 30 g of ethylene glycol, 8 g of sodium benzoate, and water to a final volume of 1000 g.

3. The application according to claim 1 or 2, characterized in that: Apply the herbicide once during the three-branch stage of ginger, using a spray at a dosage of 112.5~300 grams of active ingredient per hectare.

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