A liquid-based sheet making apparatus

Abstract

The application relates to the technical field of liquid-based sheet making, and particularly discloses a liquid-based sheet making device. The liquid-based sheet making device comprises an execution main body, a control system and a bearing platform with a sedimentation position, the execution main body comprises a mixing mechanism, a centrifugal mechanism, a first conveying assembly and a second conveying assembly; the control system is used for controlling the execution main body to execute at least a first sheet making process and a second sheet making process; each sheet making process comprises steps of sequentially performing elution mixing, centrifugation and sedimentation on a sample, and the second sheet making process is started to be executed after the elution mixing step of the first sheet making process is completed and before the sedimentation step is completed; the first sheet making process is used for sheet making on a first sample, and the second sheet making process is used for sheet making on a second sample. The liquid-based sheet making device provided by the application can simultaneously execute two or more sheet making processes, so that the utilization rate and the sheet making efficiency of the liquid-based sheet making device can be effectively improved.

Description

Technical Field

[0001] This invention relates to the field of liquid-based tablet preparation technology, and more particularly to a liquid-based tablet preparation device. Background Technology

[0002] Currently, cervical cancer ranks second among female malignant tumors, after breast cancer, posing a serious threat to women's health. Cervical cancer screening is divided into three stages: early screening, colposcopy, and tissue biopsy. Early screening primarily uses cervical cytology and human papillomavirus (HPV) testing. Cervical cytology is a screening test mainly used to detect squamous cell carcinoma and precancerous lesions. In addition, cytology is also used to diagnose ovarian function. HPV testing cannot be used alone for cervical cancer screening. Its main limitation is that how to further evaluate numerous positive HPV test results is currently unclear. Furthermore, while HPV testing is more sensitive (true positive rate) than cytology testing, its specificity (true negative rate) is lower. Therefore, current cervical cancer screening primarily relies on cytology, supplemented by HPV testing.

[0003] Liquid-based cytology, developed in 1991, is currently one of the most advanced cervical cancer screening technologies internationally, initially applied to gynecological cytology examinations. Due to its advanced nature, liquid-based sample processing equipment has gradually been introduced to the market. However, existing liquid-based sample processing equipment typically operates in a single-cycle manner. This process cannot fully utilize the various functional components of the equipment, resulting in a high idle rate and low slide preparation efficiency. Summary of the Invention

[0004] The purpose of this invention is to provide a liquid-based film preparation device, which aims to at least solve the problems of high idle rate of functional components and low film preparation efficiency in existing liquid-based film preparation devices.

[0005] To achieve the above objectives, the technical solutions adopted in the embodiments of the present invention are as follows:

[0006] A liquid-based tablet preparation device, comprising:

[0007] The execution body includes a mixing mechanism, a centrifugation mechanism, a first conveying assembly, and a second conveying assembly;

[0008] The support platform has a settlement position;

[0009] A control system, wherein the control system is used to control the executing entity to execute at least a first film production process and a second film production process;

[0010] The first slide preparation process includes the following steps in sequence: first elution and mixing, first centrifugation, and first sedimentation.

[0011] The first elution and mixing step includes controlling the first delivery component to deliver the first sample to the mixing mechanism for elution and mixing;

[0012] The first centrifugation step includes controlling the first delivery assembly to deliver the first sample, which has been eluted and mixed, to the centrifugation mechanism for centrifugation.

[0013] The first sedimentation step includes controlling the second conveying component to place the sedimentation component at the sedimentation position, and controlling the first conveying component to convey the first sample after centrifugation to the sedimentation component for sedimentation treatment;

[0014] The second slide preparation process includes the following steps in sequence: second elution and mixing, second centrifugation, and second sedimentation.

[0015] The second elution and mixing step includes controlling the first delivery component to deliver the second sample to the mixing mechanism for elution and mixing;

[0016] The second centrifugation step includes controlling the first delivery assembly to deliver the eluted and mixed second sample to the centrifugation mechanism for centrifugation.

[0017] The second sedimentation step includes controlling the second conveying component to place the sedimentation component at the sedimentation position, and the first conveying component to convey the second sample after centrifugation to the sedimentation component for sedimentation treatment;

[0018] After the first elution and mixing step is completed and before the first sedimentation step is completed, the execution entity is controlled to start the second film preparation process.

[0019] In some embodiments, the control system is also used to control the execution entity to perform a third film production process;

[0020] The third slide preparation process includes sequentially performing a third elution and mixing, a third centrifugation, and a third sedimentation:

[0021] The third elution and mixing step includes controlling the first delivery component to deliver the third sample to the mixing mechanism for elution and mixing.

[0022] The third centrifugation step includes controlling the first delivery component to deliver the eluted and mixed third sample to the centrifugation mechanism for centrifugation.

[0023] The third sedimentation step includes controlling the second conveying component to place the sedimentation component at the sedimentation position, and the first conveying component to convey the third sample after centrifugation to the sedimentation component for sedimentation treatment;

[0024] The third film preparation process is initiated after the second elution and mixing step is completed and before the second sedimentation step is completed.

[0025] In some implementations, the second slide preparation process is initiated during the first centrifugation of the first sample;

[0026] And / or, the third slide preparation process is initiated during the second centrifugation process of the second sample.

[0027] In some embodiments, the execution body further includes a dyeing mechanism and a post-processing mechanism;

[0028] After controlling each film preparation process to perform a sedimentation step, the control system further includes controlling the staining mechanism to stain the sedimentation-treated samples and controlling the staining-treated samples to undergo post-processing in the post-processing mechanism; or, it further includes controlling the sedimentation-treated samples to undergo post-processing in the post-processing mechanism.

[0029] In some embodiments, the control system also controls the second conveying component to transfer the sedimentation-treated sample to the post-processing unit, or controls the second conveying component to transfer the staining-treated sample to the post-processing unit.

[0030] In some embodiments, the post-processing unit includes a first soaking unit and a second soaking unit, wherein the first soaking unit is used to hold an ethanol solution and the second soaking unit is used to hold a xylene solution; the sample obtained by the staining treatment is sequentially subjected to a first soaking treatment in the first soaking unit and the second soaking unit; or the sample obtained by the sedimentation treatment is subjected to a second soaking treatment in the first soaking unit.

[0031] In some embodiments, the execution body further includes a transport channel for transferring the sample treated with the first immersion treatment and / or the sample treated with the second immersion treatment to the outside of the liquid-based film preparation equipment.

[0032] In some embodiments, the executing entity further includes a loading mechanism movably mounted on the conveying channel for holding the samples treated with the first immersion treatment and / or the samples treated with the second immersion treatment, and for conveying them by the conveying channel.

[0033] In some embodiments, the carrier platform further has a cache bit, and the post-processing mechanism is located in the cache bit;

[0034] The orthographic projection of the conveying channel onto the upper surface of the bearing platform and the settling position are respectively located on opposite sides of the buffer position.

[0035] In some embodiments, the second transport assembly includes a second robotic arm assembly for transferring the settling assembly, slide basket, and sample-attached slides involved in each slide preparation process.

[0036] In some embodiments, the second robotic arm assembly includes a third gripper, a fourth gripper, and a fifth gripper, the third gripper being used to transfer sedimentation components involved in each slide preparation process, the fourth gripper being used to transfer slide baskets involved in each slide preparation process, and the fifth gripper being used to transfer slides with samples attached obtained in each slide preparation process.

[0037] In some embodiments, the settling assembly involved in each slide preparation process includes a base, a glass slide, a settling tube, and a seal, wherein the settling tube is movably connected to the base, the glass slide is movably inserted between the base and the settling tube, and the seal is used to seal the connection between the settling tube and the glass slide.

[0038] The execution body also includes a disassembly mechanism. The third gripper is used to transfer the sedimentation component after sedimentation treatment or the sedimentation component after staining treatment to the disassembly mechanism for disassembly to obtain a glass slide with the sample attached. The fifth gripper then transfers the disassembled glass slide with the sample attached to the post-processing mechanism.

[0039] In some embodiments, the first conveying assembly includes a first robotic arm assembly and a suction / discharge assembly;

[0040] The first robotic arm assembly is used to grasp and transfer sample containers, and to grasp and transfer centrifuge tubes;

[0041] The aspiration and expulsion assembly is used to add the eluted and mixed sample into a centrifuge tube containing a gradient separation solution, and to transfer the centrifuged sample to a sedimentation assembly placed in the sedimentation position.

[0042] In some embodiments, the first robotic arm assembly includes a first gripper and a second gripper, the first gripper being used to grasp and transfer the sample container; the second gripper being used to grasp and transfer the centrifuge tube; the suction and discharge assembly includes a drive mechanism and a suction and discharge connector, the drive mechanism being used to drive the suction and discharge connector to suction and discharge the sample.

[0043] In some embodiments, the aspiration / dispensing connector includes a pipette tip and a pipette needle, the pipette tip being used to transfer the eluted and mixed sample into a centrifuge tube; the pipette needle being used to transfer the centrifuged sample into the sedimentation assembly for sedimentation.

[0044] In some implementations, each slide preparation process includes, before the elution and mixing step, controlling the first gripper to transfer a sample container containing the sample to the mixing mechanism; and between the elution and mixing step and the centrifugation step, controlling the aspiration assembly to transfer the eluted and mixed sample to a centrifuge tube and controlling the first gripper to transfer the centrifuge tube containing the sample to the centrifugation mechanism.

[0045] The first delivery assembly further includes a first drive arm, and the first gripper and the suction and discharge assembly are both installed on the first drive arm. When the first slide preparation process is in the centrifugation step, the first drive arm drives the first gripper to load the sample container containing the second sample into the mixing mechanism for the second elution and mixing treatment, or the first drive arm drives the first gripper to transfer the second sample after elution and mixing treatment into the centrifuge tube.

[0046] In some embodiments, the executing entity further includes a liquid path component, which is controlled by the control system to add gradient separation solution to the centrifuge tube before sample addition and to add buffer solution to the centrifuge tube after centrifugation; or to control the liquid path component to aspirate the supernatant generated by the centrifuge tube during centrifugation.

[0047] In some embodiments, a portion of the fluid circuit assembly is connected to the first delivery assembly.

[0048] In some embodiments, the executing entity further includes a scanning mechanism and a coding mechanism. The scanning mechanism is used to scan the sample container and / or centrifuge tube, and the coding mechanism is used to code the sedimentation components before they are sent to the sedimentation position in each slide preparation process, so that the information of the sedimentation components matches the information of the sample container and / or centrifuge tube.

[0049] In some implementations, each slide preparation process includes, after the elution and mixing step and before the centrifugation step, the sequential addition of a gradient separation solution and the sample obtained from the elution and mixing treatment to a centrifuge tube.

[0050] In some embodiments, the executing entity further includes a carrier device for loading centrifuge tubes, and the control system controls the carrier device to tilt the centrifuge tubes to a preset angle, and then adds gradient separation liquid and the sample obtained by the elution and mixing treatment to the centrifuge tubes.

[0051] In some implementations, before performing the next film preparation process, the method further includes controlling the first delivery assembly to remove the sample container from the mixing mechanism in the previous film preparation process.

[0052] In some implementations, the centrifugation step of each slide preparation process is repeated at least twice, and each centrifugation is followed by a step of removing the supernatant from the centrifuge tube.

[0053] In some embodiments, the centrifuge mechanism is located at the bottom of the support platform, which has a clearance through hole. In each film preparation process, a centrifuge tube is fed into the centrifuge mechanism through the clearance through hole for centrifugation, or the centrifuge tube is removed from the centrifuge mechanism through the clearance through hole.

[0054] Compared to existing technologies, the liquid-based slide preparation equipment provided in this invention includes an execution body and a control system. The execution body includes a mixing mechanism, a centrifugation mechanism, and a first conveying component. The control system controls the execution body to perform at least a first slide preparation process for a first sample and a second slide preparation process for a second sample. Each slide preparation process includes controlling the first conveying component to transfer the sample to the mixing mechanism for elution and mixing, controlling the first conveying component to convey the eluted and mixed sample to the centrifugation mechanism for centrifugation, and controlling the second conveying component to place the sedimentation component in the sedimentation position and controlling the first conveying component to transfer the centrifuged sample to the sedimentation component for sedimentation. The second slide preparation process is started after the first slide preparation process has completed the elution and mixing step and before the sedimentation step. This reduces the idle rate of each component in the execution body of the liquid-based slide preparation equipment, fully utilizes each component, thereby improving the utilization rate of the liquid-based slide preparation equipment and effectively increasing the efficiency of slide preparation. Attached Figure Description

[0055] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0056] Figure 1 This is a simplified schematic diagram of a liquid-based tableting apparatus provided in an embodiment of the present invention;

[0057] Figure 2 A simplified schematic diagram of the film preparation process of the liquid-based film preparation equipment provided in this embodiment of the invention;

[0058] Figure 3 A simplified structural diagram of the liquid circuit assembly of the liquid-based tablet fabrication equipment provided in an embodiment of the present invention;

[0059] Figure 4 A simplified schematic diagram of the execution subject provided in the embodiments of the present invention;

[0060] Figure 5 A simplified schematic diagram of the control relationship of the liquid-based tableting equipment provided in this embodiment of the invention;

[0061] Figure 6 A top view of the liquid-based tableting equipment provided in an embodiment of the present invention after removing the housing, the first conveying assembly, and the second conveying assembly;

[0062] Figure 7 for Figure 6 A magnified view of a portion of point A in the middle;

[0063] Figure 8 for Figure 6 A magnified view of a portion of point B in the middle;

[0064] Figure 9 A three-dimensional structural diagram of the liquid-based tableting equipment provided in an embodiment of the present invention after removing the housing, the first conveying assembly, and the second conveying assembly;

[0065] Figure 10 for Figure 9 A magnified view of a portion of point C in the middle;

[0066] Figure 11 This is a three-dimensional structural schematic diagram of the liquid-based tableting equipment provided in an embodiment of the present invention;

[0067] Figure 12 for Figure 11 A magnified view of a portion of point D in the middle;

[0068] Figure 13 A three-dimensional structural schematic diagram of the liquid-based film preparation equipment provided in an embodiment of the present invention from another perspective;

[0069] Figure 14 for Figure 13 A magnified view of a portion of point E in the middle;

[0070] Figure 15 This is a schematic diagram of the exploded structure of the settling component provided in an embodiment of the present invention.

[0071] Figure label:

[0072] 1. Liquid-based tablet preparation equipment; 100. Receiving cavity; 101. First discharge port; 102. Second discharge port; 103. Third discharge port;

[0073] 10. Implementing entity;

[0074] 11. Mixing mechanism;

[0075] 12. Centrifuge mechanism;

[0076] 13. First conveying assembly; 131. First robotic arm assembly; 1311. First gripper; 1312. Second gripper; 132. Suction / discharge assembly; 1321. Suction / discharge connector; 1322. Suction / discharge body; 133. First drive arm;

[0077] 14. Dyeing facilities;

[0078] 15. Post-treatment mechanism; 151. First soaking mechanism; 152. Second soaking mechanism;

[0079] 16. Second conveying assembly; 161. Second robotic arm assembly; 1611. Third gripper; 1612. Fourth gripper; 1613. Fifth gripper;

[0080] 17. Conveying channel; 171. Loading mechanism;

[0081] 18. Disassembly mechanism; 181. Scanning mechanism; 182. Coding mechanism; 183. Supporting device; 184. Lifting mechanism;

[0082] 19. Fluid circuit assembly; 191. First pipeline; 192. Second pipeline; 193. Third pipeline; 194. Fourth pipeline; 195. Fifth pipeline;

[0083] 20. Support platform; 21. Settlement position; 22. Buffer position; 23. Clearance through hole; 24. Recycling port;

[0084] 30. Control system;

[0085] 40. Splitting mechanism;

[0086] 50. Settling assembly; 51. Base; 52. Glass slide; 53. Settling tube; 54. Sealing element;

[0087] 60. Sample container; 70. Centrifuge tube; 80. Slide basket. Detailed Implementation

[0088] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0089] The structure of the liquid-based film-making device 1 and the film-making process of the liquid-based film-making device 1 provided in this embodiment are as follows: Figures 1 to 15 As shown.

[0090] Please see Figure 1 , Figure 4 , Figure 5 as well as Figure 2 The liquid-based slide preparation equipment 1 includes an execution body 10, a support platform 20, and a control system 30. The execution body 10 includes a mixing mechanism 11, a centrifugation mechanism 12, a first conveying component 13, and a second conveying component 16. The support platform 20 has a settling position 21. The control system 30 controls the execution body 10 to perform at least a first slide preparation process and a second slide preparation process. The first slide preparation process includes sequentially performing a first elution mixing, a first centrifugation, and a first settling step. In the first slide preparation process, the first elution mixing step includes controlling the first conveying component 13 to convey a first sample to the mixing mechanism 11 for elution mixing; the first centrifugation step includes controlling the first conveying component 13 to convey the eluted and mixed first sample to the centrifugation mechanism 12 for centrifugation; the first settling step includes controlling the second conveying component 16 to place the settling component 50 at the settling position 21, and controlling the first conveying component 13 to convey the centrifuged first sample to the settling component 50 for settling. The second slide preparation process includes sequentially performing a second elution mixing, a second centrifugation, and a second settling step. The second film preparation process is initiated after the execution body 10 completes the first elution and mixing step and before the first sedimentation step. In the second film preparation process, the second elution and mixing step includes controlling the first conveying component 13 to convey the second sample to the mixing mechanism 11 for elution and mixing; the second centrifugation step includes controlling the first conveying component 13 to convey the eluted and mixed second sample to the centrifugation mechanism 12 for centrifugation; the second sedimentation step includes controlling the second conveying component 16 to place the sedimentation component 50 at the sedimentation position 21, and controlling the first conveying component 13 to convey the centrifuged second sample to the sedimentation component 50 for sedimentation. In this embodiment, the process is initiated at any point after the first elution and mixing step is completed and before the first sedimentation step is completed, allowing multiple components of the execution body 10 to work simultaneously, reducing the idle rate of the execution body 10, thereby effectively improving the utilization rate and efficiency of the liquid-based film preparation equipment 1. In some implementations, the second slide preparation process is initiated during the first centrifugation of the first sample, which can effectively simplify the structure of the first transport assembly 13, allowing the first transport assembly 13 to perform at least two slide preparation processes simultaneously without complex control and transport relationships.

[0091] Please see Figure 2 , Figure 4 and Figure 5In some embodiments, the control system 30 is also used to control the execution entity 10 to execute a third film preparation process, and the third film preparation process is started after the completion of the second elution and mixing step and before the completion of the second sedimentation step. Specifically, the third film preparation process includes the steps of performing a third elution and mixing, a third centrifugation, and a third sedimentation in sequence. In the third film preparation process, the third elution and mixing step includes controlling the first conveying component 13 to convey the third sample to the mixing mechanism 11 for elution and mixing; the third centrifugation step includes controlling the first conveying component 13 to convey the eluted and mixed third sample to the centrifugation mechanism 12 for centrifugation; the third sedimentation step includes controlling the second conveying component 16 to place the sedimentation component 50 at the sedimentation position 21, and controlling the first conveying component 13 to convey the centrifuged third sample to the sedimentation component 50 for sedimentation. In some embodiments, the third film preparation process is started during the second centrifugation of the second sample. When the third film preparation process is started during the centrifugation of the second sample, the control and conveying relationships of the first conveying component 13 can also be effectively simplified, that is, at least two film preparation processes can be performed simultaneously.

[0092] Please see Figure 13 , Figure 14 , Figure 7 and Figure 1 In some embodiments, the first delivery assembly 13 includes a first robotic arm assembly 131 and a suction / expulsion assembly 132, wherein the sample is contained in a sample container 60 for delivery into the liquid-based slide preparation apparatus 1. The first robotic arm assembly 131 is used to grasp and transfer the sample container 60, and simultaneously, the first robotic arm assembly 131 is used to grasp and transfer the centrifuge tube 70; the suction / expulsion assembly 132 is used to add the eluted and mixed sample to the centrifuge tube 70 containing the gradient separation liquid, and to transfer the centrifuged sample to the sedimentation assembly 50 placed in the sedimentation position 21.

[0093] Please see Figure 14 , Figure 1 and Figure 2 In some embodiments, the first robotic arm assembly 131 includes a first gripper 1311 and a second gripper 1312. The first gripper 1311 is used to grasp and transfer the sample container 60; the second gripper 1312 is used to grasp and transfer the centrifuge tube 70; the suction / explosion assembly 132 includes a drive mechanism (not shown in the figure), a suction / explosion body 1322, and a suction / explosion connector 1321. After the suction / explosion body 1322 is inserted into the suction / explosion connector 1321, the drive mechanism drives the suction / explosion connector 1321 to suction / explode the sample.

[0094] Please see Figure 14 , Figure 1 and Figure 2In some embodiments, the aspiration connector 1321 includes a pipette tip (not shown) and a pipette needle (not shown). The pipette tip is used to transfer the eluted and mixed sample into the centrifuge tube 70 to add the sample into the centrifuge tube 70; the pipette needle is used to transfer the centrifuged sample into the sedimentation assembly 50 for sedimentation.

[0095] In each slide preparation process, before the elution and mixing step, the first gripper 1311 is controlled to transfer the sample container 60 containing the sample to the mixing mechanism 11; between the elution and mixing step and the centrifugation step, the aspiration and dispensing assembly 132 is controlled to transfer the eluted and mixed sample into the centrifuge tube 70 and the first gripper 1311 is controlled to transfer the centrifuge tube 70 containing the sample into the centrifugation mechanism 12.

[0096] Please see Figure 14 In some embodiments, the first conveying assembly 13 further includes a first driving arm 133. A first gripper 1311 and a suction / exposure assembly 132 are both mounted on the first driving arm 133. When the first slide preparation process is in the centrifugation step, the first driving arm 133 drives the first gripper 1311 to load the sample container 60 containing the second sample into the mixing mechanism 11 for the second elution and mixing treatment, or the first driving arm 133 drives the first gripper 1311 to transfer the eluted and mixed second sample into the centrifuge tube 70. In some embodiments, there are two first driving arms 133. The first gripper 1311 and part of the suction / exposure assembly 132 are mounted on one first driving arm 133, while the remaining suction / exposure assembly 132 are mounted on the other first driving arm 133. This structural design allows the first conveying assembly 13 to simultaneously perform conveying along two different paths. In some embodiments, the first gripper 1311 and the second gripper 1312 are both mounted on the same first drive arm 133, while in other embodiments, each first drive arm 133 is respectively equipped with the first gripper 1311 and the second gripper 1312.

[0097] Please see Figure 4 , Figure 5 , Figure 12In some embodiments, the execution body 10 further includes a staining mechanism 14 and a post-processing mechanism 15. After the control system 30 controls each slide preparation process to execute the sedimentation step, it also includes steps of controlling the staining mechanism 14 to stain the sedimentation-treated sample and controlling the stained sample to undergo post-processing in the post-processing mechanism 15. In other embodiments, after the control system 30 controls each slide preparation process to execute the sedimentation step, it also includes steps of controlling the sedimentation-treated sample to undergo post-processing in the post-processing mechanism 15. In both embodiments, the former can obtain a stained sample, while the latter obtains only the sample that has settled on the slide 52. Therefore, after each slide preparation process reaches the sedimentation step, it is possible to obtain two types of samples to facilitate post-processing and analysis according to different needs.

[0098] Please see Figure 7 In some embodiments, the post-processing unit 15 includes a first soaking unit 151 and a second soaking unit 152. The first soaking unit 151 holds an ethanol solution, and the second soaking unit 152 holds a xylene solution. In some embodiments, if a stained sample is required, the stained sample is sequentially subjected to a first soaking treatment in the first soaking unit 151 and then in the second soaking unit 152. In other embodiments, if an unstained sample is required, the sample obtained after sedimentation treatment only needs to undergo a second soaking treatment in the first soaking unit 151. In some embodiments, when the first soaking unit 151 performs both the first and second soaking treatments, the ethanol solution in the first soaking unit 151 needs to be replaced to avoid cross-contamination. For example, when switching from the first soaking process to the second soaking process, the ethanol solution in the first soaking unit 151 should be replaced to avoid contamination of the sample in the second soaking process due to residual staining solution in the sample. When replacing the ethanol solution in the first soaking unit 151, the unit should be cleaned at least once with ethanol solution to minimize or even eliminate the residual amount of staining solution in the first soaking unit 151. Similarly, when switching from the second soaking process to the first soaking process, it is also necessary to replace the ethanol solution in the first soaking unit 151.

[0099] In some embodiments, the first soaking mechanism 151 has two or more first soaking tanks (not shown in the figure), some of which are used for the first soaking treatment, while the remaining second soaking tanks are used for the second soaking treatment. When the first soaking mechanism 151 has multiple first soaking tanks for both the first and second soaking treatments, cross-contamination will not occur in the first soaking mechanism 151. In this case, it is not necessary to replace the ethanol solution in the first soaking mechanism 151. Therefore, ethanol solution can be added to the first soaking tank after each soaking process has been repeated multiple times. In some embodiments, the first soaking mechanism 151 has a cover plate (not shown in the figure) covering the opening of the first soaking tank to prevent the evaporation and diffusion of the ethanol solution. In some embodiments, the second soaking mechanism 152 also has a cover plate (not shown in the figure) covering the opening of the second soaking mechanism 152 to prevent the evaporation and diffusion of the xylene solution.

[0100] Please see Figure 4 , Figure 5 , Figure 1 In some embodiments, the execution body 10 further includes a conveying channel 17, which is used to transfer samples that have undergone the first immersion treatment to the outside of the liquid-based slide preparation device 1, or to transfer samples that have undergone the second immersion treatment to the outside of the liquid-based slide preparation device 1, or to transfer samples that have undergone the first immersion treatment and samples that have undergone the second immersion treatment to the outside of the liquid-based slide preparation device 1 simultaneously. In some embodiments, the conveying channel 17 is located on the top of the support platform 20, that is, the conveying channel 17 is connected to the support platform 20 or there is a gap between the conveying channel 17 and the support platform 20. Regardless of whether the conveying channel 17 is connected to the support platform 20 or there is a gap, the orthographic projection of the conveying channel 17 onto the support platform 20 falls within the area enclosed by the support platform 20.

[0101] Please see Figure 1 , Figure 9In some embodiments, the executing entity 10 further includes a loading mechanism 171, which is movably mounted on the conveying channel 17 for holding samples that have undergone the first immersion treatment and conveying them via the conveying channel 17, or for holding samples that have undergone the second immersion treatment and conveying them via the conveying channel 17, or for simultaneously holding samples that have undergone the first and second immersion treatments and conveying them via the conveying channel 17. In some embodiments, the loading mechanism 171 contains an ethanol solution or a xylene solution. When the loading mechanism 171 is used to transport samples that have undergone the first immersion treatment, it contains a xylene solution; when the loading mechanism 171 is used to transport samples that have undergone the second immersion treatment, it contains an ethanol solution. In some embodiments, the loading mechanism 171 has two or more receiving slots (not shown in the figure), wherein some receiving slots are used to hold ethanol solution for transporting samples that have undergone the second immersion treatment, and the remaining receiving slots are used to hold xylene solution for transporting samples that have undergone the first immersion treatment. In some embodiments, the loading mechanism 171 is provided with a cover plate (not shown in the figure) which covers the opening end of the receiving tank to cover the ethanol solution or xylene solution and reduce the evaporation and diffusion of the ethanol solution or xylene solution.

[0102] Please see Figure 1 In some embodiments, the carrier platform 20 further includes a buffer position 22, where the post-processing mechanism 15 is located. The orthographic projection of the transport channel 17 onto the upper surface of the carrier platform 20 and the settling position 21 are respectively located on opposite sides of the buffer position 22. In some embodiments, the area enclosed by the orthographic projection of the transport channel 17 onto the upper surface of the carrier platform 20 has a gap with the buffer position 22, or they may be adjacent to each other without a gap. This structural design can effectively shorten the sample transfer distance, thereby effectively reducing the lateral dimension of the liquid-based slide preparation equipment 1.

[0103] Please see Figure 1 , Figure 12 In some embodiments, the liquid-based slide preparation apparatus 1 has a first outlet 101, a second outlet 102, and a third outlet 103. The first outlet 101 is connected to the conveying channel 17, and the second outlet 102 is used both for discharging material and for feeding a slide basket 80 into the liquid-based slide preparation apparatus 1. The first outlet 101 and the third outlet 103 can be connected to other devices to achieve automatic sample delivery, such as connecting the first outlet 101 to a DNA ploidy analysis device, and connecting the third outlet 103 to a sealing machine.

[0104] Please see Figure 12 , Figure 4 and Figure 5In some embodiments, the second conveying assembly 16, under the control of the control system 30, can transfer the sample obtained after sedimentation treatment to the post-processing mechanism 15; or, under the control of the control system 30, the second conveying assembly 16 can transfer the sample obtained after staining treatment to the post-processing mechanism 15; or, under the control of the control system 30, the second conveying assembly 16 can transfer the slide basket 80 to the post-processing mechanism 15; or transfer the post-processed sample to the loading mechanism 171, the second discharge port 102, and the third discharge port 103.

[0105] Please see Figure 12 , Figure 4 and Figure 5 In some embodiments, the second transport assembly 16 includes a second robotic arm assembly 161 for transferring the transfer settling assembly 50, the slide basket 80, and the slide 52 with the sample attached to each slide preparation process.

[0106] Please see Figure 12 , Figure 15 In some embodiments, the second robotic arm assembly 161 includes a third gripper 1611, a fourth gripper 1612, and a fifth gripper 1613, each gripper in the second robotic arm assembly 161 being driven by a power mechanism (not shown in the figure). The third gripper 1611 is used to transfer the settling assembly 50, the fourth gripper 1612 is used to transfer the slide basket 80, and the fifth gripper 1613 is used to transfer the slide 52 with the sample attached. In some embodiments, the settling assembly 50 involved in each slide preparation process includes a base 51, a slide 52, a settling tube 53, and a seal 54. The settling tube 53 is movably connected to the base 51, the slide 52 is movably inserted between the base 51 and the settling tube 53, and the seal 54 is used to seal the connection between the settling tube 53 and the slide 52. The third gripper 1611 first transfers the sedimentation assembly 50 placed in the liquid-based slide preparation equipment 1 to code the sedimentation assembly 50, thus encoding the glass slide 52 within it. Next, the coded sedimentation assembly 50 is transferred to the sedimentation position 21. Subsequently, the first conveying assembly 13 transfers the centrifuged sample to the sedimentation assembly 50 for sedimentation, causing the sample to adhere to the surface of the glass slide 52. The third gripper 1611 then transfers the sedimentation assembly 50 for disassembly, separating the glass slide 52 from the sedimentation tube 53, base 51, and seal 54. Finally, the fifth gripper 1613 transfers the disassembled glass slide 52 to the post-processing mechanism 15. In some embodiments, the third gripper 1611 transfers the stained sedimentation assembly 50 for disassembly.

[0107] Please see Figure 10 , Figure 5 , Figure 4 and Figure 12 In some embodiments, the execution body 10 further includes a disassembly mechanism 18, wherein a third gripper 1611 is used to transfer the sedimentation component 50 after sedimentation treatment or the sedimentation component 50 after staining treatment to the disassembly mechanism 18 for disassembly to obtain a glass slide 52 with the sample attached, and the glass slide 52 with the sample attached obtained by the disassembly by the fifth gripper 1613 is transferred to the post-processing mechanism 15. Specifically, the third gripper 1611 transfers the sedimentation component 50, after sedimentation treatment or after staining treatment, to the disassembly mechanism 18. The disassembly mechanism 18 includes a disassembly body (not shown in the figure) and a rotary drive (not shown in the figure). The disassembly body has a fixed position (not shown in the figure). After the sedimentation component 50 is fixed in the fixed position of the disassembly mechanism 18, the third gripper 1611 rotates to drive the sedimentation tube 53 to rotate, thereby separating the sedimentation tube 53 from the base 51. Subsequently, the rotary drive drives the disassembly body to rotate, causing the slide 52 to rotate from the horizontal direction to the vertical direction. The fifth gripper 1613 then grasps the vertically positioned slide 52 and transfers it to the post-processing mechanism 15. During the transfer of the slide 52 to the post-processing mechanism 15, the rotary drive drives the disassembly body to continue rotating, causing the base 51 to move from the disassembly mechanism 18... The component detaches from its fixed position, completing the entire disassembly process. The remaining settling components 50 are disassembled in the same manner.

[0108] Please see Figure 7 , Figure 10 , Figure 12 In some embodiments, before transferring the slide 52 to the post-processing mechanism 15, the slide basket 80 fed into the liquid-based slide preparation equipment 1 is first transferred to the post-processing mechanism 15 by the fourth gripper 1612, so that the slide 52 is inserted into the slide basket 80 to complete the immersion treatment. Then, the fourth gripper 1612 grabs the slide basket 80 to transfer the immersion-treated sample to the loading mechanism 171, the second discharge port 102, or the third discharge port 103. By transferring the slide 52 through the slide basket 80, a batch of slides 52 can be transferred at once, that is, multiple samples can be transferred at once. This not only effectively improves the efficiency of sample transportation but also reduces the energy consumption of sample transportation and simplifies the control process of sample transportation.

[0109] Please see Figure 3 , Figure 4 , Figure 5 as well as Figure 14In some embodiments, the execution entity 10 further includes a liquid path assembly 19, which is controlled by the control system 30 to add gradient separation solution to the centrifuge tube 70 before sample addition, add buffer solution to the centrifuge tube 70 after centrifugation, or aspirate the supernatant generated in the centrifuge tube 70 during centrifugation. In some embodiments, the liquid path assembly 19 includes a first tube 191, a second tube 192, a third tube 193, a fourth tube 194, and a fifth tube 195. The first tube 191 is used to deliver the gradient separation solution to the centrifuge tube 70; the second tube 192 is used to deliver the buffer solution to the centrifuge tube 70 after centrifugation; the third tube 193 is used to deliver an ethanol solution; the fourth tube 194 is used to deliver a xylene solution; and the fifth tube 195 is used to aspirate the supernatant generated during centrifugation. In some embodiments, a portion of the liquid path assembly 19 is connected to the first drive arm 133, thereby enabling the first drive arm 133 to drive the liquid path assembly 19 to add or aspirate liquid. In some embodiments, a portion of the liquid path assembly 19 is connected to the first delivery assembly 13 to facilitate adding or aspirating liquid. For example, the first conduit 191 is connected to the first delivery assembly 13, and the first delivery assembly 13 moves the first conduit 191 to add gradient separation liquid to the centrifuge tube 70; the second conduit 192 is connected to the first delivery assembly 13, and the first delivery assembly 13 moves the second conduit 192 to add buffer solution to the centrifuge tube 70; the fifth conduit 195 is connected to the first delivery assembly 13, and the first delivery assembly 13 moves the fifth conduit 195 to aspirate the supernatant from the centrifuge tube 70.

[0110] Please see Figure 4 , Figure 5 and Figure 6 In some embodiments, the execution body 10 further includes a scanning mechanism 181, which is used to scan the sample container 60 containing the sample to obtain relevant information about the sample container 60, and at the same time to scan the centrifuge tube 70 before it is filled with gradient separation liquid to obtain relevant information about the centrifuge tube 70.

[0111] Please see Figure 1 , Figure 4 , Figure 5 , Figure 6 In some embodiments, the execution body 10 further includes a coding mechanism 182, which is used to code the sedimentation component 50 before it is sent into the sedimentation position 21, so that the information of the sedimentation component 50 matches the information of the sample container 60 and the centrifuge tube 70.

[0112] Please see Figure 3 , Figure 5 , Figure 10 , Figure 14 In some embodiments, each film preparation process, after the elution and mixing step is completed and before the centrifugation step, includes sequentially adding gradient separation liquid and the eluted and mixed sample to the centrifuge tube 70. That is, the control system 30 controls the first pipeline 191 in the liquid path assembly 19 to add gradient separation liquid to the centrifuge tube 70, and controls the aspiration and dispensing assembly 132 to remove the eluted and mixed sample, and inject the eluted and mixed sample into the centrifuge tube 70 containing the gradient separation liquid.

[0113] Please see Figure 10 , Figure 5 , Figure 6 In some embodiments, the execution body 10 further includes a support device 183 for loading centrifuge tubes 70. The control system 30 controls the support device 183 to tilt the centrifuge tubes 70 to a preset angle, and then adds gradient separation liquid and the sample obtained after elution and mixing to the centrifuge tubes 70. In some embodiments, when the support device 183 tilts the centrifuge tubes 70, the preset angle is 0° to 60°, that is, the angle between the central axis of the centrifuge tubes 70 and the vertical direction is 0° to 60°.

[0114] Please see Figure 1 , Figure 5 , Figure 8 In some embodiments, before performing the next film preparation process, the method further includes controlling the first delivery assembly 13 to remove the sample container 60 placed in the mixing mechanism 11 in the previous film preparation process.

[0115] Please see Figure 6 , Figure 9 , Figure 1 , Figure 4 , Figure 5 , Figure 14 In some embodiments, the centrifuge mechanism 12 is located at the bottom of the support platform 20, which has a clearance through-hole 23. During each slide preparation process, centrifuge tubes 70 are fed into the centrifuge mechanism 12 through the clearance through-hole 23 for centrifugation, or centrifuge tubes 70 are removed from the centrifuge mechanism 12 through the clearance through-hole 23. In some embodiments, the execution body 10 also includes a lifting mechanism 184, which is connected to the support platform 20 or the centrifuge mechanism 12 to transfer centrifuge tubes 70 into or remove centrifuge tubes 70 from the centrifuge mechanism 12. During operation, the second gripper 1312 transfers the centrifuge tubes 70 to the lifting mechanism 184, which then delivers the centrifuge tubes 70 to the centrifuge mechanism 12. After centrifugation, the lifting mechanism 184 removes the centrifuge tubes 70 from the centrifuge mechanism 12, and the suction and discharge assembly 132 aspirates the sample from the centrifuge tubes 70 and transfers it to the sedimentation assembly 50.

[0116] In some implementations, the centrifugation step of each slide preparation process is repeated at least twice, and each centrifugation is completed by removing the supernatant from the centrifuge tube 70. Through multiple centrifugation processes, a good centrifugation effect can be obtained.

[0117] Please see Figure 1 , Figure 6 , Figure 8 In some embodiments, the support platform 20 is provided with a recovery port 24 for recovering the aspiration / exposure connector 1321 after sample aspiration / exposure. In some embodiments, the liquid-based slide preparation apparatus 1 further includes a splitting mechanism 40 for splitting the aspiration / exposure body 1322 and the aspiration / exposure connector 1321, thereby causing the aspiration / exposure connector 1321 to detach from the aspiration / exposure body 1322 and fall into the recovery port 24. In some embodiments, the splitting mechanism 40 is connected to the support platform 20 and is located near the recovery port 24.

[0118] Please see Figure 1 , Figure 6 , Figure 9 , Figure 10 , Figure 11 , Figure 13 as well as Figure 4 In some embodiments, the liquid-based film preparation equipment includes a support frame (not shown in the figure) and a housing (not shown in the figure). The housing is mounted on the support frame, which supports the housing and together with the housing forms a receiving cavity 100. The execution body 10, the support platform 20, the control system 30, and the splitting mechanism 40 are all housed in the receiving cavity 100. The support platform 20 divides the receiving cavity 100 into an upper space and a lower space. The mixing mechanism 11, the first conveying assembly 13, the staining mechanism 14, the post-processing mechanism 15, and the second conveying assembly are also included. 16. The conveying channel 17, loading mechanism 171, disassembly mechanism 18, scanning mechanism 181, coding mechanism 182, carrying device 183, and splitting mechanism 40 are all housed in the upper space, while the centrifugation mechanism 12 is housed in the lower space. The lifting mechanism 184 uses lifting motion to transfer the centrifuge tube 70 containing the gradient separation liquid and sample in the upper space to the lower space and load it into the centrifugation mechanism 12, or the lifting mechanism 184 uses lifting motion to transfer the centrifuge tube 70 in the centrifugation mechanism 12 to the upper space.

[0119] Please see Figure 2 as well as Figure 1 , Figures 3 to 15 The basic working process of the liquid-based tablet preparation equipment 1 provided in this embodiment is as follows:

[0120] (I) First Production Process:

[0121] Centrifuge tube 70, suction and discharge connector 1321, and sample container 60 containing the first sample are added to the liquid-based slide preparation equipment 1.

[0122] The control system 30 controls the first gripper 1311 to grab the sample container 60 containing the first sample and transfer it to the barcode scanning mechanism 181 for scanning to obtain information about the sample container 60.

[0123] The control system 30 controls the first gripper 1311 to transfer the scanned sample container 60 to the mixing mechanism 11 for elution and mixing, so that the first sample contained in the sample container 60 is eluted and evenly dispersed.

[0124] While the mixing mechanism 11 is eluting and mixing the first sample, the control system 30 controls the second gripper 1312 to grab the centrifuge tube 70 and transfer it to the scanning mechanism 181 for scanning processing to obtain the information of the centrifuge tube 70.

[0125] The second gripper 1312 is controlled to transfer the centrifuge tube 70 into the carrier device 183;

[0126] The control bearing device 183 is tilted so that the angle between the central axis of the centrifuge tube 70 and the vertical direction is between 0° and 60°, and the control liquid circuit assembly 19 injects the gradient separation liquid into the centrifuge tube 70.

[0127] Control the aspiration and expulsion body 1322 to be inserted into the aspiration and expulsion connector 1321, and control the aspiration and expulsion assembly 132 to aspirate the first sample after elution and mixing, and then inject it into the centrifuge tube 70 containing the gradient separation liquid; control the aspiration and expulsion assembly 132 to move to the recovery port 24, and separate the aspiration and expulsion body 1322 and the aspiration and expulsion connector 1321, so that the aspiration and expulsion connector 1321 falls into the recovery port 24;

[0128] The control device 183 is restored to a vertical position, and the first gripper 1311 is controlled to transfer the centrifuge tube 70 containing the gradient separation liquid and the first sample to the centrifugation mechanism 12;

[0129] The centrifugation mechanism 12 is controlled to centrifuge the first sample loaded in the centrifuge tube 70, and the first gripper 1311 is controlled to remove the sample container 60 from the mixing mechanism 11.

[0130] When the centrifuge mechanism 12 completes the first centrifugation process, the control liquid path assembly 19 draws the supernatant in the centrifuge tube 70, and controls the centrifuge mechanism 12 to perform the second centrifugation process and controls the control liquid path assembly 19 to draw the supernatant generated in the centrifuge tube 70 after the second centrifugation process.

[0131] Control the first gripper 1311 to remove the centrifuge tube 70 from the centrifugation mechanism 12, and control the liquid circuit assembly 19 to add buffer solution to the centrifuge tube 70 after centrifugation;

[0132] The third gripper 1611 controls the settling component 50 to be transferred to the coding mechanism 182 for coding processing, and then the coded settling component 50 is transferred to the settling position 21.

[0133] The aspiration and dispensing body 1322 is connected to the aspiration and dispensing connector 1321, and the first sample in the buffer solution is aspirated and transferred to the sedimentation assembly 50 placed in the sedimentation position 21 for sedimentation treatment. The first sample is attached to the surface of the glass slide 52. The aspiration and dispensing assembly 132 is moved to the recovery port 24, and the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321 are separated, so that the aspiration and dispensing connector 1321 falls into the recovery port 24.

[0134] The staining mechanism 14 controls the staining process of the first sample obtained from sedimentation.

[0135] The third gripper 1611 is controlled to transfer the stained sedimentation assembly 50 to the disassembly mechanism 18 for disassembly, so that the sedimentation tube 53 is separated from the glass slide 52; at the same time, the fourth gripper 1612 is controlled to grab the glass slide basket 80 in the liquid-based slide preparation equipment 1 and transfer it to the first immersion mechanism 151 or the second immersion mechanism 152.

[0136] The fifth gripper 1613 controls the disassembled glass slide 52 to be sequentially transferred to the first immersion mechanism 151 and the second immersion mechanism 152 for the first immersion treatment;

[0137] The fourth gripper 1612 is controlled to grab the slide basket 80 in the second soaking mechanism 152 to transfer the sample after the first soaking treatment to the loading mechanism 171, and the sample is transferred to the first discharge port 101 by the conveying channel 17, or the sample after the first soaking treatment is transferred to the second discharge port 102 or the third discharge port 103.

[0138] (II) Second film preparation process: When the first film preparation process reaches any point between centrifugation and sedimentation, the second film preparation process is initiated. The second film preparation process is as follows:

[0139] Add centrifuge tube 70, suction and discharge connector 1321 and sample container 60 containing the second sample to liquid-based slide preparation equipment 1;

[0140] The control system 30 controls the first gripper 1311 to grab the sample container 60 containing the second sample and transfer it to the barcode scanning mechanism 181 for scanning to obtain information about the sample container 60.

[0141] The control system 30 controls the first gripper 1311 to transfer the scanned sample container 60 to the mixing mechanism 11 for elution and mixing, so that the second sample contained in the sample container 60 is eluted and evenly dispersed.

[0142] When the mixing mechanism 11 washes and mixes the second sample, the control system 30 controls the second gripper 1312 to grab the centrifuge tube 70 and transfer it to the scanning mechanism 181 for scanning processing to obtain the information of the centrifuge tube 70.

[0143] The second gripper 1312 is controlled to transfer the centrifuge tube 70 into the carrier device 183;

[0144] The control bearing device 183 is tilted so that the angle between the central axis of the centrifuge tube 70 and the vertical direction is between 0° and 60°, and the control liquid circuit assembly 19 injects the gradient separation liquid into the centrifuge tube 70.

[0145] Control the aspiration and expulsion body 1322 to be inserted into the aspiration and expulsion connector 1321, and control the aspiration and expulsion assembly 132 to aspirate the second sample after elution and mixing, and then inject it into the centrifuge tube 70 containing the gradient separation liquid; control the aspiration and expulsion assembly 132 to move to the recovery port 24, and separate the aspiration and expulsion body 1322 and the aspiration and expulsion connector 1321, so that the aspiration and expulsion connector 1321 falls into the recovery port 24;

[0146] The control device 183 is restored to a vertical position, and the first gripper 1311 is controlled to transfer the centrifuge tube 70 containing the gradient separation liquid and the second sample to the centrifugation mechanism 12;

[0147] The centrifugation mechanism 12 is controlled to centrifuge the second sample loaded in the centrifuge tube 70, while the first gripper 1311 is controlled to remove the sample container 60 used to hold the second sample from the mixing mechanism 11.

[0148] When the centrifuge mechanism 12 completes the first centrifugation process, the control liquid path assembly 19 draws the supernatant in the centrifuge tube 70, and controls the centrifuge mechanism 12 to perform the second centrifugation process and controls the control liquid path assembly 19 to draw the supernatant generated in the centrifuge tube 70 after the second centrifugation process.

[0149] Control the first gripper 1311 to remove the centrifuge tube 70 from the centrifugation mechanism 12, and control the liquid circuit assembly 19 to add buffer solution to the centrifuge tube 70 after centrifugation;

[0150] The third gripper 1611 controls the settling component 50 to be transferred to the coding mechanism 182 for coding processing, and then the coded settling component 50 is transferred to the settling position 21.

[0151] The aspiration and dispensing body 1322 is connected to the aspiration and dispensing connector 1321, and the second sample in the buffer solution is aspirated and transferred to the sedimentation assembly 50 placed in the sedimentation position 21 for sedimentation treatment, so that the second sample adheres to the glass slide 52; the aspiration and dispensing assembly 132 is moved to the recovery port 24, and the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321 are separated, so that the aspiration and dispensing connector 1321 falls into the recovery port 24;

[0152] The staining mechanism 14 controls the staining process of the second sample obtained from sedimentation.

[0153] The third gripper 1611 is controlled to transfer the stained sedimentation assembly 50 to the disassembly mechanism 18 for disassembly, so that the sedimentation tube 53 is separated from the glass slide 52; at the same time, the fourth gripper 1612 is controlled to grab the glass slide basket 80 in the liquid-based slide preparation equipment 1 and transfer it to the first immersion mechanism 151 or the second immersion mechanism 152.

[0154] The fifth gripper 1613 controls the disassembled glass slide 52 to be sequentially transferred to the first immersion mechanism 151 and the second immersion mechanism 152 for the first immersion treatment;

[0155] The fourth gripper 1612 is controlled to grab the slide basket 80 in the second soaking mechanism 152 to transfer the sample after the first soaking treatment to the loading mechanism 171, and the sample is transferred to the first discharge port 101 by the conveying channel 17, or the sample after the first soaking treatment is transferred to the second discharge port 102 or the third discharge port 103.

[0156] (iii) At any point between centrifugation and sedimentation in the second film preparation process, the third film preparation process is started. The third film preparation process is the same as the first and second film preparation processes. The difference is that the third film preparation process uses a third sample. The detailed process of the third film preparation process will not be elaborated here.

[0157] In some implementations, samples from the first, second, and third slide preparation processes do not require further staining after sedimentation treatment. Figure 2 In the diagram, the staining step is within the dashed box, indicating that the sample after sedimentation treatment is directly subjected to the post-processing step. Therefore, in each slide preparation process, after disassembling the sedimentation component 50 to obtain the glass slide 52, the fifth gripper 1613 transfers the glass slide 52 to the first immersion mechanism 151 for the second immersion treatment. After the second immersion treatment, the sample is transferred by the fourth gripper 1612 to the loading mechanism 171, and then transferred to the first discharge port 101 by the conveying channel 17.

[0158] It should be noted that in the technical solution of this invention, the terms "first sample," "second sample," and "third sample" do not necessarily indicate that they are different. They can be samples from the same batch, divided into first, second, and third samples for the convenience of liquid-based slide preparation equipment 1. Alternatively, they can indicate that the first, second, and third samples are different from each other or partially the same. When there are more samples, the slide preparation process described above can be followed to fully utilize the liquid-based slide preparation equipment 1 and reduce the idle rate of various components in the equipment. In addition, the samples involved include test cells, such as cervical exfoliated cells. After sample processing, the test cells eventually settle and adhere to the glass slide 52 to facilitate subsequent observation and analysis, thereby obtaining the state of the test cells in the sample.

[0159] Example 1

[0160] Please see Figure 2 , combined Figure 1 , Figures 3 to 15 In this embodiment, a first sample, a second sample, and a third sample are involved. The first sample consists of eight samples, the second sample consists of eight samples, and the third sample also consists of eight samples. The first sample is produced according to the first production process, the second sample is produced according to the second production process, and the third sample is produced according to the third production process. The specific steps are as follows:

[0161] (1) The first gripper 1311 sequentially grips the sample container 60 containing the first sample, scans them respectively, and transfers them to the mixing mechanism 11 for elution and mixing. While the first sample is being eluted and mixed, the second gripper 1312 sequentially grips eight centrifuge tubes 70, scans them respectively, and transfers the centrifuge tubes 70 to the carrier device 183. The carrier device 183 is controlled to tilt the centrifuge tubes 70 so that the angle between the central axis of the centrifuge tubes 70 and the vertical direction is 45°. The first pipeline 191 in the liquid circuit assembly 19 is controlled to add gradient separation liquid to each centrifuge tube 70 in the tilted state.

[0162] (2) Control the insertion of the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321. The drive mechanism drives the aspiration and dispensing connector 1321 to pick up the first sample after elution and mixing and add it to the centrifuge tube 70 which is tilted. One first sample is added to each centrifuge tube 70. The drive support device 183 is restored to the vertical state. After each sample is added to the centrifuge tube 70, the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321 are separated to replace the new aspiration and dispensing connector 1321 to pick up another first sample. The separated aspiration and dispensing connector 1321 falls into the recovery port 24.

[0163] (3) The second gripper 1312 transfers the centrifuge tube 70 containing the first sample and gradient separation liquid to the lifting mechanism 184. The lifting mechanism 184 then transfers the centrifuge tube 70 containing the first sample and gradient separation liquid to the centrifuge mechanism 12. After placing it in a centrifugation balance manner, the centrifuge mechanism 12 is started to perform the first centrifugation treatment on the centrifuge tube 70 containing the first sample and gradient separation liquid.

[0164] (4) While the first sample is centrifuged for the first time, the first gripper 1311 is controlled to take out the sample container 60 containing the first sample from the mixing mechanism 11. After the first centrifugation of the first sample is completed, the fifth pipeline 195 in the liquid circuit assembly 19 is controlled to draw the supernatant from the centrifuge tube 70.

[0165] (5) Centrifuge the centrifuge tube 70 containing the first sample and gradient separation liquid for the second time. At the same time, the first gripper 1311 grabs the second sample, scans it, and transfers it to the mixing mechanism 11 for elution and mixing. While the second sample is being eluted and mixed, the second gripper 1312 grabs eight centrifuge tubes 70 in sequence, scans them, and transfers the centrifuge tubes 70 to the carrier device 183. The carrier device 183 is controlled to tilt the centrifuge tubes 70 so that the angle between the central axis of the centrifuge tubes 70 and the vertical direction is 45°. The first pipeline 191 in the liquid path assembly 19 is controlled to add gradient separation liquid to each centrifuge tube 70 in the tilted state.

[0166] (6) Control the insertion of the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321. The drive mechanism drives the aspiration and dispensing connector 1321 to pick up the second sample after elution and mixing, and add it to the centrifuge tube 70 which is tilted. One second sample is added to each centrifuge tube 70. The drive support device 183 is restored to the vertical state. After each sample is added to the centrifuge tube 70, the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321 are separated to replace the new aspiration and dispensing connector 1321 to pick up another second sample. The separated aspiration and dispensing connector 1321 falls into the recovery port 24.

[0167] (7) After the second centrifugation of the first sample is completed, the fifth tube 195 in the control liquid circuit assembly 19 aspirates the supernatant in the centrifuge tube 70, and the lifting mechanism 184 removes the centrifuge tube 70 containing the first sample and transfers it to the centrifuge tube 70 placement position. The second tube 192 in the liquid circuit assembly 19 adds buffer solution to the centrifuge tube 70 containing the first sample, and the first sample is transferred to the sedimentation assembly 50 placed in the sedimentation position 21 through the aspiration and expulsion assembly 132. At the same time, the second gripper 1312 transfers the centrifuge tube 70 containing the second sample and gradient separation liquid to the lifting mechanism 184. The lifting mechanism 184 transfers the centrifuge tube 70 containing the second sample and gradient separation liquid to the centrifugation mechanism 12. After placing it in the centrifugation equilibrium manner, the centrifugation mechanism 12 is started to perform the first centrifugation of the centrifuge tube 70 containing the second sample and gradient separation liquid.

[0168] (8) While the second sample is being centrifuged for the first time, the first gripper 1311 places the sample container 60 containing the second sample in the mixing mechanism 11; and the first sample is being settled in the sedimentation assembly 50; after the second sample is finished being centrifuged for the first time, the fifth pipeline 195 in the control liquid path assembly 19 draws the supernatant from the centrifuge tube 70.

[0169] (9) The second sample is centrifuged a second time. At the same time, the first gripper 1311 is controlled to grab the third sample, scan it, and transfer it to the mixing mechanism 11 for elution and mixing. While the third sample is being eluted and mixed, the second gripper 1312 grabs eight centrifuge tubes 70 in sequence, scans them, and transfers them to the carrier device 183. The carrier device 183 is controlled to tilt the centrifuge tubes 70 so that the angle between the central axis of the centrifuge tubes 70 and the vertical direction is 45°. The first pipeline 191 in the liquid path assembly 19 is controlled to add gradient separation liquid to each centrifuge tube 70 in the tilted state. During this time period, the staining mechanism 14 stains the first sample after sedimentation.

[0170] (10) Control the insertion of the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321. The drive mechanism drives the aspiration and dispensing connector 1321 to pick up the third sample after elution and mixing, and add it to the centrifuge tube 70 which is tilted. One third sample is added to each centrifuge tube 70. The drive support device 183 is restored to the vertical state. After each sample is added to the centrifuge tube 70, the aspiration and dispensing body 1322 and the aspiration and dispensing connector 1321 are separated to replace the new aspiration and dispensing connector 1321 to pick up another third sample. The separated aspiration and dispensing connector 1321 falls into the recovery port 24.

[0171] (11) After the second centrifugation of the second sample is completed, the first tube 191 in the control liquid path assembly 19 aspirates the supernatant in the centrifuge tube 70, and the lifting mechanism 184 removes the centrifuge tube 70 containing the second sample and transfers it to the centrifuge tube 70 placement position. The second tube 192 in the control liquid path assembly 19 adds buffer solution to the centrifuge tube 70 containing the second sample, and the second sample is transferred to the sedimentation assembly 50 placed in the sedimentation position 21 by the aspiration and expulsion assembly 132. At the same time, the second gripper 1312 transfers the centrifuge tube 70 containing the third sample and gradient separation liquid to the lifting mechanism 184. The lifting mechanism 184 transfers the centrifuge tube 70 containing the third sample and gradient separation liquid to the centrifugation mechanism 12. After placing it in the centrifugation equilibrium manner, the centrifugation mechanism 12 is started to perform the first centrifugation of the centrifuge tube 70 containing the third sample and gradient separation liquid.

[0172] (12) While the third sample is being centrifuged for the first time, the first gripper 1311 places the sample container 60 containing the third sample in the mixing mechanism 11; and the second sample is being settled in the sedimentation assembly 50; after the first centrifugation of the third sample is completed, the first pipeline 191 in the control liquid path assembly 19 draws the supernatant in the centrifuge tube 70; and the third gripper 1611 transfers the stained first sample for tube disassembly, the fourth gripper 1612 transfers the glass slide 52 obtained from tube disassembly to the post-processing mechanism 15 for the first soaking treatment, and the third gripper 1611 transfers the first sample that has undergone the first soaking treatment to the loading mechanism 171 or the second discharge port 102 or the third discharge port 103;

[0173] (13) The third sample is centrifuged a second time. After the second centrifugation is completed, the first tube 191 in the liquid path assembly 19 draws the supernatant obtained from the second centrifugation. The centrifuge tube 70 containing the third sample is taken out by the lifting mechanism 184. The second tube 192 in the liquid path assembly 19 adds buffer solution to the centrifuge tube 70 and then transfers it to the sedimentation assembly 50 placed in the sedimentation position 21. At the same time, the staining mechanism 14 stains the second sample.

[0174] (14) While the third sample was undergoing sedimentation treatment, the second sample was undergoing tube disassembly treatment and first soaking treatment.

[0175] (15) The third sample after sedimentation was stained, disassembled and subjected to the first soaking treatment.

[0176] The above are merely specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and these modifications or substitutions should all be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A liquid-based tablet preparation device, characterized in that, include: The execution body includes a mixing mechanism, a centrifugation mechanism, a post-processing mechanism, a first conveying assembly, a second conveying assembly, and a disassembly mechanism; The support platform has a settlement position; A control system, wherein the control system is used to control the executing entity to execute at least a first film production process, a second film production process, and a third film production process; Each slide preparation process includes at least the steps of elution and mixing, centrifugation, sedimentation, and post-processing in sequence; the elution and mixing step includes controlling the first conveying component to convey the sample to the mixing mechanism for elution and mixing; the centrifugation step includes controlling the first conveying component to convey the eluted and mixed sample to the centrifugation mechanism for centrifugation; the sedimentation step includes controlling the second conveying component to place the sedimentation component at the sedimentation position, and controlling the first conveying component to convey the centrifuged sample to the sedimentation component for sedimentation. The control system further includes a step of controlling the sedimentation-treated sample to undergo post-processing in the post-processing unit; or, the executing entity further includes a staining unit, and after controlling each slide preparation process to perform a sedimentation step, the control system further includes a step of controlling the staining unit to stain the sedimentation-treated sample and controlling the staining-treated sample to undergo post-processing in the post-processing unit. The second conveying assembly includes a second robotic arm assembly, which includes a third gripper and a fifth gripper. The control system further controls the third gripper to transfer the sedimentation assembly after sedimentation treatment or the sedimentation assembly after staining treatment to the disassembly mechanism for disassembly to obtain a glass slide with the sample attached, and controls the fifth gripper to transfer the disassembled glass slide with the sample attached to the post-processing mechanism. After the elution and mixing step is completed in the first film preparation process and before the sedimentation step is completed, the execution entity is controlled to start executing the second film preparation process. The third film preparation process is started after the second film preparation process has completed the elution and mixing step and before the sedimentation step is completed. The first conveying assembly includes a first robotic arm assembly and a suction / discharge assembly; The first robotic arm assembly is used to grasp and transfer sample containers, and to grasp and transfer centrifuge tubes; the first robotic arm assembly includes a first gripper, which is used to grasp and transfer the sample containers. The first delivery assembly further includes a first drive arm, and the first gripper and the suction and discharge assembly are both mounted on the first drive arm. When the first slide preparation process is in the centrifugation step, the first drive arm drives the first gripper to load the sample container containing the second sample in the second slide preparation process into the mixing mechanism for the second elution and mixing treatment, or the first drive arm drives the first gripper to transfer the second sample that has undergone elution and mixing treatment in the second slide preparation process into the centrifuge tube.

2. The liquid-based tablet preparation equipment as described in claim 1, characterized in that, The third film preparation process is initiated and executed during the centrifugation process of the second sample in the second film preparation process.

3. The liquid-based tablet preparation equipment as described in claim 1, characterized in that, The post-processing unit includes a first soaking unit and a second soaking unit. The first soaking unit is used to hold an ethanol solution, and the second soaking unit is used to hold a xylene solution. The sample obtained by the staining treatment is subjected to a first soaking treatment in the first soaking unit and the second soaking unit in sequence; or the sample obtained by the sedimentation treatment is subjected to a second soaking treatment in the first soaking unit.

4. The liquid-based tablet preparation equipment as described in claim 3, characterized in that, The execution body also includes a conveying channel for transferring samples treated with the first immersion treatment and / or samples treated with the second immersion treatment to the outside of the liquid-based film preparation equipment.

5. The liquid-based tablet preparation equipment as described in claim 4, characterized in that, The execution body further includes a loading mechanism, which is movably installed in the conveying channel for holding samples that have undergone the first soaking treatment and / or samples that have undergone the second soaking treatment, and for conveying them by the conveying channel.

6. The liquid-based tablet preparation equipment as described in claim 5, characterized in that, The carrier platform also has a cache slot, and the post-processing mechanism is located in the cache slot; The orthographic projection of the conveying channel onto the upper surface of the bearing platform and the settling position are respectively located on opposite sides of the buffer position.

7. The liquid-based tablet preparation equipment as described in claim 1, characterized in that, The second robotic arm assembly is used to transfer the settling components, slide baskets, and slides with attached samples involved in each slide preparation process.

8. The liquid-based tablet preparation equipment as described in claim 7, characterized in that, The second robotic arm assembly includes the third gripper, the fourth gripper, and the fifth gripper. The third gripper is used to transfer the settling components involved in each slide preparation process, the fourth gripper is used to transfer the slide basket involved in each slide preparation process, and the fifth gripper is used to transfer the slide with the sample attached obtained in each slide preparation process.

9. The liquid-based tablet preparation equipment as described in claim 8, characterized in that, Each slide preparation process involves a settling assembly including a base, a glass slide, a settling tube, and a seal. The settling tube is movably connected to the base, the glass slide is movably inserted between the base and the settling tube, and the seal is used to seal the connection between the settling tube and the glass slide.

10. The liquid-based tablet preparation equipment as described in claim 1 or 2, characterized in that, The aspiration and expulsion assembly is used to add the eluted and mixed sample into a centrifuge tube containing a gradient separation solution, and to transfer the centrifuged sample to a sedimentation assembly placed in the sedimentation position.

11. The liquid-based tablet preparation equipment as described in claim 10, characterized in that, The first robotic arm assembly further includes a second gripper for gripping and transferring the centrifuge tube; the suction and discharge assembly includes a drive mechanism and a suction and discharge connector, the drive mechanism for driving the suction and discharge connector to suction and discharge the sample.

12. The liquid-based tablet preparation equipment as described in claim 11, characterized in that, The aspiration and dispensing connector includes a pipette tip and a pipette needle. The pipette tip is used to transfer the eluted and mixed sample into a centrifuge tube. The pipette needle is used to transfer the centrifuged sample into the sedimentation assembly for sedimentation.

13. The liquid-based tablet preparation equipment as described in claim 12, characterized in that, In each slide preparation process, before the elution and mixing step, the first gripper is controlled to transfer the sample container containing the sample to the mixing mechanism; between the elution and mixing step and the centrifugation step, the aspiration and dispensing assembly is controlled to transfer the eluted and mixed sample to a centrifuge tube, and the first gripper is controlled to transfer the centrifuge tube containing the sample to the centrifugation mechanism.

14. The liquid-based tablet preparation equipment as described in claim 1 or 2, characterized in that, The execution entity also includes a liquid path component, and the control system controls the liquid path component to add gradient separation solution to the centrifuge tube before adding the sample and to add buffer solution to the centrifuge tube after centrifugation. Alternatively, the liquid circuit assembly can be controlled to draw the supernatant produced by the centrifuge tube during centrifugation.

15. The liquid-based tablet preparation equipment as described in claim 14, characterized in that, The liquid circuit assembly is partially connected to the first delivery assembly.

16. The liquid-based tablet preparation equipment as described in claim 1 or 2, characterized in that, The execution entity also includes a scanning mechanism and a coding mechanism. The scanning mechanism is used to scan the sample container and / or centrifuge tube; the coding mechanism is used to code the sedimentation components before they are sent to the sedimentation position in each slide preparation process, so that the information of the sedimentation components matches the information of the sample container and / or centrifuge tube.

17. The liquid-based tablet preparation equipment as described in claim 1 or 2, characterized in that, In each slide preparation process, after the elution and mixing step is completed and before the centrifugation step is performed, the gradient separation solution and the sample obtained by the elution and mixing process are sequentially added to the centrifuge tube.

18. The liquid-based tablet preparation equipment as described in claim 17, characterized in that, The execution body also includes a carrier device for loading centrifuge tubes. The control system controls the carrier device to tilt the centrifuge tubes to a preset angle, and then adds gradient separation liquid and the sample obtained by the elution and mixing treatment into the centrifuge tubes.

19. The liquid-based tablet preparation equipment as described in claim 1 or 2, characterized in that, Before executing the next film preparation process, the procedure also includes controlling the first delivery assembly to remove the sample container from the mixing mechanism in the previous film preparation process.

20. The liquid-based tablet preparation equipment as described in claim 1 or 2, characterized in that, Each centrifugation step in the slide preparation process is repeated at least twice, and each centrifugation is completed by removing the supernatant from the centrifuge tube.

21. The liquid-based tablet preparation equipment as described in claim 20, characterized in that, The centrifuge mechanism is located at the bottom of the support platform. The support platform is provided with a clearance through hole. In each film preparation process, a centrifuge tube is fed into the centrifuge mechanism through the clearance through hole for centrifugation, or the centrifuge tube is removed from the centrifuge mechanism through the clearance through hole.

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