Temperature regulation compensation device of PCR instrument and PCR instrument

Abstract

The application discloses a temperature regulation compensation device of a PCR instrument and the PCR instrument, wherein the temperature regulation compensation device of the PCR instrument comprises a temperature control module, a hot cover, a control module and a first temperature sensor, wherein a first groove on the temperature control module and a second groove on the hot cover form an accommodation space for accommodating sample tubes, so that the sample tubes can adjust the temperature in the accommodation space; the control module is used for controlling the temperature of the temperature control module, and then the control module can control the temperature of the first column body, and can play a main heating purpose; the control module heats the second column body through a heating component, and since the second column body is in contact with the first column body, the second column body can also transfer its heat to the first column body, and can play an auxiliary heating purpose, so that the temperature compensation of the sample tubes can be realized, the temperature in the plurality of first column bodies is relatively uniform, and then the temperature uniformity of the plurality of sample tubes at the same time can be good, and finally the accuracy of the detection result of the sample tubes is good.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, specifically to a temperature control compensation device for a PCR instrument and a PCR instrument. Background Technology

[0002] A PCR instrument, or polymerase chain reaction analyzer, can be broadly categorized into two types based on its modular composition: one consisting solely of a temperature control device, and the other combining a temperature control device with an optical detection device. The primary function of the temperature control device is to provide a suitable temperature environment for the in vitro DNA replication of the sample. Specifically, the in vitro DNA replication process involves: at high temperature, DNA denaturation occurs, breaking down the double strands into single strands; at low temperature, the single-stranded DNA binds to primers in the sample tube according to the principle of complementary base pairing; and at the optimal reaction temperature of the polymerase, the polymerase completes the DNA replication. During this process, the temperature control device can precisely control the denaturation, annealing, and extension temperatures, ensuring the sample can complete in vitro DNA replication. Repeating these three reaction steps multiple times can significantly increase the amount of trace DNA. Precise temperature control of the sample is crucial during these three steps; otherwise, the research results will be biased, potentially leading to detection failure.

[0003] Existing PCR instruments often exhibit significant temperature differences among multiple samples during heating or cooling processes, resulting in poor temperature uniformity across these samples at any given time. Therefore, improving temperature uniformity among multiple samples during the testing process is a pressing issue that needs to be addressed. Summary of the Invention

[0004] The purpose of this invention is to provide a temperature control compensation device for a PCR instrument and a PCR instrument in general, which solves the problem of poor temperature uniformity.

[0005] To achieve the above objectives, the present invention provides a technical solution: A temperature control and compensation device for a PCR instrument, comprising: Temperature control module, the temperature control module includes a first column, and a first groove is formed inside the first column; A heating cap includes a second column, which is movably fitted onto the outer wall of a first column, and a second groove is formed inside the second column. The second groove and the first groove form a receiving space for accommodating a sample tube, so that the sample tube can be regulated in the receiving space. A light-transmitting hole is provided on the heating cap, and the light-transmitting hole is coaxially arranged with the second groove. The control module is used to control the temperature of the temperature control module, and the control module heats the second column through a heating component, the second column is in contact with the first column, and transfers heat to the first column; A first temperature sensor is used to detect the temperature inside the first column. The first temperature sensor is used to transmit the detection signal to the control module, and the control module controls the temperature of the temperature control module and / or the temperature of the second column. The sample tubes are multiple, and adjacent sample tubes are connected by rods, so that the multiple sample tubes are arranged in a matrix. An opening is opened on the side wall of the first column, and when the sample tube is placed in the first groove of the first column, the rod passes through the opening.

[0006] Optionally, the number of the second columns is less than or equal to the number of the first columns, the number of heating components is the same as the number of the second columns, and the heating components are disposed on the second columns for heating the second columns.

[0007] Optionally, the heating components are arranged in a matrix.

[0008] Optionally, the control module controls the heating components to heat in groups; or, The control module controls the heating components to heat up one by one.

[0009] Optionally, the control module controls the heating element to heat from one side to the other in sequence.

[0010] Optionally, the heating power of the heating element gradually increases from the inside to the outside.

[0011] Optionally, there are multiple first columns, and adjacent first columns are connected by a connecting part, the top of which is lower than the top of the first column.

[0012] Optionally, the temperature control module has a first mounting slot at its bottom, which is used to place the first temperature sensor.

[0013] Optionally, a second temperature sensor is also included, which is located on top of the heat cover.

[0014] This invention provides a technical solution: A PCR instrument, characterized in that it includes the temperature control and compensation device of the PCR instrument described above.

[0015] Compared with the prior art, the beneficial effects of the present invention are: 1. The temperature regulation and compensation device of the PCR instrument of the present invention includes a temperature control module, a heating cap, a control module, and a first temperature sensor. A first groove on the temperature control module and a second groove on the heating cap form a receiving space for accommodating sample tubes, allowing the sample tubes to adjust their temperature within the receiving space. The control module controls the temperature of the temperature control module, thereby controlling the temperature of the first column, achieving the primary heating purpose. The control module heats at least a portion of the second column through a heating element. Since the second column is in contact with the first column, it can transfer its own heat to the first column, achieving auxiliary heating. This provides temperature compensation for the sample tubes. By using the first column of the temperature control module and the second column on the heating cap to jointly heat the sample tubes, the temperatures of multiple sample tubes tend to be consistent at the same time, thus solving the problem of temperature issues in existing PCR instruments. There is a significant temperature difference and poor temperature uniformity among multiple sample tubes to be tested. The first temperature sensor is used to detect the temperature inside the first column, i.e., the temperature of the sample tube. The first temperature sensor can then transmit the detection signal to the control module, which controls the temperature control module and / or the temperature of the second column. Specifically, during the heating process of the first column controlled by the control module, when the first temperature sensor senses that the internal temperature of some parts of the first column is low, that is, the temperature inside some parts of the first column has not reached the preset temperature, the control module can control the temperature of the corresponding second column. The second column then transmits the temperature to the first column, raising the temperature of the first column to the target temperature. This makes the temperature inside the multiple first columns more uniform, thus ensuring better temperature uniformity among multiple sample tubes at the same time, and ultimately improving the accuracy of the sample tube detection results.

[0016] 2. The temperature control compensation device of the PCR instrument of the present invention has a light-transmitting hole on the heat cover. The light-transmitting hole is coaxially arranged with the second groove. The purpose of the light-transmitting hole is to allow the excitation light to pass through and reach the sample tube, so as to excite the fluorescent material in the sample tube to generate emission light. The emission light is finally detected by the optical detection module to obtain the experimental results.

[0017] 3. The sample tubes of the temperature control compensation device of the PCR instrument of the present invention are connected by rods, so that multiple sample tubes are arranged in a matrix. The side wall of the first column has an opening. When the sample tube containing the sample to be tested is placed in the first groove of the first column during the PCR experiment, the rod passes through the opening. The rod and the opening can be used in conjunction so that when the heat cap is fastened to the temperature control module, the second column can be accurately fitted on the outer periphery of the first column.

[0018] 4. The temperature control compensation device of the PCR instrument of the present invention sets a second column on the hot cover and sets rods between sample tubes to connect multiple sample tubes into a whole. An opening is made on the side wall of the first column of the temperature control module. When the sample tube is placed in the first column, the second column on the hot cover can be easily connected or separated from the first column. The temperature of the first column and the second column is controlled by the control module, so that the temperature of multiple sample tubes can be made consistent at the same time, which improves the temperature uniformity among multiple sample tubes and also improves the accuracy of experimental results.

[0019] 5. The first column of the temperature control compensation device of the PCR instrument of the present invention is connected through a connecting part, and a weight reduction hole is provided between adjacent connecting parts. The weight reduction hole can reduce the weight of the entire temperature control module.

[0020] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0021] Figure 1 This is an exploded view of the temperature control and compensation device of the PCR instrument of the present invention; Figure 2 This is a first-view structural schematic diagram of the temperature control module of the temperature regulation and compensation device for the PCR instrument of the present invention. Figure 3 This is a schematic diagram of the temperature control and compensation device of the PCR instrument of the present invention after installation. Figure 4 This is a first-view structural schematic diagram of the thermal cover of the temperature control compensation device for the PCR instrument of the present invention. Figure 5 This is a cross-sectional view of the temperature control and compensation device of the PCR instrument of the present invention after installation. Figure 6 This is a front view of the heat cover of the temperature control compensation device of the PCR instrument of the present invention. Figure 7 This is a schematic diagram of the thermal cover of the temperature control compensation device of the PCR instrument of the present invention from a second perspective. Figure 8 This is a schematic diagram of the structure of the temperature control compensation device of the PCR instrument of the present invention after the sample tubes are connected together; Figure 9 This is a schematic diagram of the sample tube being installed into the temperature control module of the temperature regulation compensation device of the PCR instrument of the present invention. Figure 10 This is a second-view structural schematic diagram of the temperature control module of the temperature regulation and compensation device of the PCR instrument of the present invention.

[0022] In the picture: 1. Temperature control module; 11. First column; 111. First groove; 112. Opening; 12. Weight reduction hole; 13. First mounting groove; 2. Heat cover; 21. Second column; 211. Second groove; 22. Light transmission hole; 23. Second mounting groove; 3. Sample tube; 31. Rod. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0025] In existing PCR instruments, when the DNA of the sample to be tested is replicated in vitro, at high temperature (around 95°C, also known as the denaturation temperature), the DNA denatures, and the double strands open into single strands. At low temperature (around 60°C, also known as the annealing temperature), the single strands of DNA bind to the primers in the sample according to the principle of complementary base pairing. At the optimal reaction temperature of polymerase (around 72°C, also known as the extension temperature), the polymerase enables the DNA to replicate. During this process, the temperature control module can control the denaturation temperature, annealing temperature, and extension temperature, so that the sample to be tested can complete the in vitro DNA replication. However, when the sample to be tested is heated or cooled, the temperature of the multiple wells on the temperature control block of the temperature control module varies, resulting in poor temperature uniformity. As a result, in actual operation, there are large temperature differences between multiple sample tubes, leading to poor temperature uniformity among multiple samples and affecting the experimental results.

[0026] Therefore, this embodiment provides a temperature control compensation device for a PCR instrument, which solves the problem of poor temperature uniformity among samples during PCR experiments.

[0027] Reference Figure 1As shown, this disclosure provides a temperature control and compensation device for a PCR instrument, including a temperature control module 1, a heating cover 2, a control module, and a first temperature sensor (not shown in the figure). Wherein, as... Figure 2 As shown, the temperature control module 1 includes a first column 11, and a first groove 111 is formed inside the first column 11; as Figure 3 and Figure 4 As shown, the heat cover 2 includes a second column 21, which is movably fitted onto the outer wall of the first column 11, and a second groove 211 is formed inside the second column 21, as shown. Figure 5 As shown, the first groove 111 and the second groove 211 form a receiving space for accommodating the sample tube 3, allowing the sample tube 3 to adjust its temperature within the receiving space. Specifically, the control module controls the temperature of the temperature control module 1, thereby controlling the temperature of the first column 11, which serves as the primary heating element. The control module heats at least part of the second column 21 through the heating element. Since the second column 21 is in contact with the first column 11, it can transfer its own heat to the first column 11, serving as an auxiliary heating element. This provides temperature compensation for the sample tube 3. By using the first column 11 of the temperature control module 1 and the second column 21 on the heating cap 2 to heat the sample tube 3, the temperature of multiple sample tubes 3 tends to be consistent at the same time, thus solving the problem of large temperature differences and poor temperature uniformity among multiple sample tubes in existing PCR instruments. The first temperature sensor is used to detect the temperature inside the first column 11, that is, to detect the temperature of the sample tube 3. The first temperature sensor can then transmit the detection signal to the control module, which controls the temperature control module and / or the temperature of the second column 21. That is, during the process of the first column 11 being heated by the control module, when the first temperature sensor senses that the internal temperature of some parts of the first column 11 is low, that is, the temperature inside some parts of the first column 11 has not reached the preset temperature (e.g., denaturation temperature), the control module can control the temperature of the corresponding second column 21. The second column 21 then transmits the temperature to the first column 11, raising the temperature of the first column 11 to the target temperature, thereby making the temperature inside the multiple first columns 11 more uniform. This results in better temperature uniformity of the multiple sample tubes 3 at the same time, ultimately improving the accuracy of the detection results of the sample tubes 3.

[0028] Among them, such as Figure 6 and Figure 7As shown, a light-transmitting hole 22 is provided on the heated cover 2, and the light-transmitting hole 22 is coaxially arranged with the second groove 211. The purpose of the light-transmitting hole 22 is to allow excitation light to pass through to the sample tube 3. The real-time fluorescence quantitative PCR instrument includes an optical detection module and a temperature control module 1. The optical detection module provides excitation light, which passes through the light-transmitting hole 22 on the heated cover 2 to reach the sample tube 3, thereby exciting the fluorescent material in the sample tube 3 to generate emission light. The emission light is finally detected by the optical detection module to obtain the experimental results.

[0029] In addition, such as Figure 8 As shown, there are multiple sample tubes 3, and adjacent sample tubes 3 are connected by rods 31, so that the multiple sample tubes 3 are arranged in a matrix. Figure 2 As shown, an opening 112 is formed in the side wall of the first column 11, as... Figure 9 As shown, during a PCR experiment, when the sample tube 3 containing the sample to be tested is placed into the first groove 111 of the first column 11, the rod 31 passes through the opening 112. The rod 31 and the opening 112 can be used together so that when the heat cap 2 is fastened to the temperature control module 1, the second column 21 can be accurately fitted onto the outer periphery of the first column 11, that is, as shown... Figure 5 As shown. This application connects multiple sample tubes 3 into a whole by setting a second column 21 on the heating cover 2 and setting a rod 31 between the sample tubes 3. An opening 112 is opened on the side wall of the first column 11 of the temperature control module 1. When the sample tube 3 is placed in the first column 11, the second column 21 on the heating cover 2 can be easily connected or separated from the first column 11. The temperature of the first column 11 and the second column 21 is controlled by the control module, so that the temperature of multiple sample tubes 3 can be made consistent at the same time, which improves the temperature uniformity among multiple sample tubes 3 and also improves the accuracy of experimental results. The whole device has a compact structure and is easy to operate.

[0030] It should be noted that when the first temperature sensor detects that the internal temperature of most or all of the first column 11 is low, the control module can control the temperature of the temperature control module 1 to raise the temperature of the first column 11 to the target temperature.

[0031] It should be noted that the depth of the opening 112 can be adjusted according to actual needs. The deeper the opening 112, the larger the contact area between the first column 11 and the second column 21, and the higher the efficiency of the auxiliary heating of the second column 21. The depth of the opening 112 refers to the distance between the top of the first column 11 and the bottom of the opening 112. The aforementioned hot cover 2 is controlled by an additional circuit system to maintain a constant temperature, so that when the hot cover 2 is fastened to the temperature control module 1, the hot cover 2 can cover the top of the sample tube 3 to prevent the sample to be tested in the sample tube 3 from evaporating or condensing backflow. After the experiment is over, the hot cover 2 is removed and the sample tube 3 is taken out from the first slot 111 of the temperature control module 1.

[0032] The aforementioned control module is equivalent to the hollow system of the temperature regulation and compensation device of the PCR instrument, and can control the operation of the entire system.

[0033] The materials of the first column 11 and the second column 21 mentioned above are thermally conductive materials, which can be thermally conductive metals or other thermally conductive materials.

[0034] In some embodiments, the number of second columns 21 is less than or equal to the number of first columns 11, and the number of heating elements is the same as the number of second columns 21. The heating elements are located on the second columns 21 and are used to heat the second columns 21. Since each second column 21 corresponds to one heating element, when the internal temperature of some first columns 11 does not reach the preset temperature, the corresponding second column 21 can be controlled by the control module to compensate for the heat of the first column 11. At this time, the corresponding heating element will heat up, causing the temperature of the second column 21 to rise.

[0035] The heating element can be either embedded inside the second column 21 or attached to the inner wall of the second column 21.

[0036] In some embodiments, there are multiple first columns 11, and adjacent first columns 11 are connected by a connecting part. The top of the connecting part is lower than the top of the first column, and a weight reduction hole is provided on the connecting part. The weight reduction hole 12 can reduce the weight of the entire temperature control module 1 and save raw material costs.

[0037] The top of the connecting part is lower than the top of the first column 11 in order to ensure that the second column 21 can be fitted onto the outer wall of the first column 11.

[0038] In some embodiments, the heating components are arranged in a matrix, and the arrangement of the heating components is the same as that of the sample tube 3, which is more conducive to heating the sample tube 3 through the first column 11 and the second column 21.

[0039] Since the heating elements and the second column 21 are arranged in a matrix, the horizontal heating elements can be a row of heating elements, and the vertical heating elements can be a column of heating elements.

[0040] The control module controls the second column 21 to provide auxiliary heating to the sample tube 3 by controlling the heating component. There are multiple methods.

[0041] The first heating method: The control module controls the heating elements to heat one by one. Specifically, when the first heating element is powered on, the others are powered off; when the second heating element is powered on, the first and other heating elements are powered off; when the third heating element is powered on, the first, second, and other heating elements are powered off, and so on. Regardless of the number of heating elements, this on-off switching method ensures that each sample tube 3 can receive individual temperature compensation through its corresponding second column 21 without being affected by heat transfer interference from other second columns 21. This results in better auxiliary heating and improved temperature uniformity among the multiple sample tubes 3.

[0042] The second heating method: The control module controls the heating components to heat in groups. At this time, the control module controls several heating components to heat (several heating components form a group). When the first group of heating components is heating, the remaining heating components are de-energized. When the second group of heating components is energized, the first group and the other heating components are de-energized, and so on. Regardless of the number of groups of heating components, the above method of power on / off is used to achieve group heating. It should be noted that the number of heating components in a group can be 2, 3, 4, 5, 6, etc., which can improve the efficiency of auxiliary heating, complete the auxiliary heating of sample tube 3 in a shorter time, and increase the experimental speed.

[0043] The third heating method: The control module controls the heating components to heat sequentially from one side to the other. At this time, the control module controls one row of heating components to heat. That is, when the first row of heating components is powered on, the other heating components are powered off. When the second row of heating components is powered on, the first row and other heating components are powered off. When the third row of heating components is powered on, the first row, the second row and other heating components are powered off, and so on. No matter how many rows of heating components are set, the above-mentioned power on and off is used to realize the heating components heating row by row from one side to the other.

[0044] Alternatively, the control module controls a series of heating components to perform heating. The heating method here is the same as described above, so it will not be repeated here.

[0045] It should be noted that the above steps only show some possible working processes of the heating element, and the heating element may have other working processes.

[0046] For example, when the number of sample tubes 3 is 96, there can be 96 second columns 21 and 96 heating elements, arranged in a matrix. The sample tubes 3 and heating elements are then correspondingly positioned. The control module can use pulse width modulation (PWM) technology to control the heating elements and is designed to complete one auxiliary heating of each of the 96 sample tubes 3 within 1 or 2 seconds. This allows for individual auxiliary heating of each of the 96 sample tubes 3 to be completed in a very short time, approximating simultaneous auxiliary heating of all 96 sample tubes 3, further improving the temperature uniformity among the multiple sample tubes 3.

[0047] In some embodiments, the heating power of the heating element gradually increases from the inside to the outside. That is, the heating power of the heating element located on the periphery is higher than that of the heating element located on the inside.

[0048] In actual use, the temperature inside the outer first column 11 will be slightly lower than the temperature inside the inner first column 11, so the heating power of the outer first column 11 can be higher.

[0049] In some embodiments, such as Figure 10 As shown, the temperature control module 1 has a first mounting slot 13 at its bottom. The first mounting slot 13 is used to place the first temperature sensor, and multiple first mounting slots 13 can be provided to install multiple first temperature sensors to detect the temperature inside the first column 11. The first temperature sensor is electrically connected to the control module, and the first temperature sensor can transmit the detected signal to the control module, which then controls the temperature of the temperature control module 1 and / or the second column 21.

[0050] The temperature control module 1 also includes a first plate, a first column 11 located above the plate, and a first mounting groove 13 formed at the bottom of the first plate. The first plate and the first column 11 can be made of the same or different materials.

[0051] like Figure 7 As shown, in some embodiments, the temperature control and compensation device of the PCR instrument further includes a second temperature sensor (not shown in the figure). The second temperature sensor is located in the second mounting slot 23 on the top of the hot cover 2 and can detect the temperature of the hot cover 2. The second temperature sensor is electrically connected to the control module and can transmit the detected signal to the control module, which controls the temperature of the hot cover 2 itself to prevent the sample in the sample tube 3 from evaporating during the heating process.

[0052] The heat cover 2 also includes a second plate, a second column 21 located below the plate, and a second mounting groove 23 formed on the top of the second plate. The second plate and the second column 21 can be made of the same or different materials.

[0053] The working process of the temperature control and compensation device of the PCR instrument: First, the temperature control module 1, sample tubes 3, and heating cap 2 are installed. Then, the control module raises the temperature to the first target temperature (approximately 95°C) by controlling the temperature control module 1, causing DNA denaturation. At this time, the first temperature sensor detects the temperature inside the first column 11 and transmits the detected temperature signal to the control module. When the first temperature sensor detects that the internal temperature of some parts of the first column 11 has not reached the first target temperature, the control module can control the temperature of the corresponding second column 21. The second column 21 then transmits the temperature to the first column 11, raising the temperature of the first column 11 to the first target temperature, thereby making the temperature inside the multiple first columns 11 more uniform, and thus ensuring good temperature uniformity of multiple sample tubes 3 at the same time. Then, the control module lowers the temperature to the second target temperature (approximately 60°C) by controlling the temperature control module 1, causing the sample to be tested in the sample tube 3 to replicate DNA according to the principle of complementary base pairing, and repeats the above temperature control process to complete the replication and amplification of the sample to be tested.

[0054] In addition, this application also provides a PCR instrument, including the temperature control compensation device of the PCR instrument described above. Since it also has the same or similar technical effects as described above, it will not be described in detail here. For details, please refer to the description of the temperature control compensation device of the PCR instrument described above.

[0055] In the description of this invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description, and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0056] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0057] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A temperature control and compensation device for a PCR instrument, characterized in that, include: Temperature control module, the temperature control module includes a first column, and a first groove is formed inside the first column; A heating cap includes a second column, which is movably fitted onto the outer wall of a first column, and a second groove is formed inside the second column. The second groove and the first groove form a receiving space for accommodating a sample tube, so that the sample tube can be regulated in the receiving space. A light-transmitting hole is provided on the heating cap, and the light-transmitting hole is coaxially arranged with the second groove. The control module is used to control the temperature of the temperature control module, and the control module heats the second column through a heating component, the second column is in contact with the first column, and transfers heat to the first column; A first temperature sensor is used to detect the temperature inside the first column. The first temperature sensor is used to transmit the detection signal to the control module, and the control module controls the temperature of the temperature control module and / or the temperature of the second column. The sample tubes are multiple, and adjacent sample tubes are connected by rods, so that the multiple sample tubes are arranged in a matrix. An opening is opened on the side wall of the first column, and when the sample tube is placed in the first groove of the first column, the rod passes through the opening. The number of the second columns is less than or equal to the number of the first columns, the number of the heating components is the same as the number of the second columns, and the heating components are disposed on the second columns for heating the second columns, and the heating components are embedded inside the second columns.

2. The temperature control and compensation device for the PCR instrument according to claim 1, characterized in that, The heating components are arranged in a matrix.

3. The temperature control and compensation device for the PCR instrument according to claim 2, characterized in that, The control module controls the heating components to heat in groups; or... The control module controls the heating components to heat up one by one.

4. The temperature control and compensation device for the PCR instrument according to claim 2, characterized in that, The control module controls the heating component to heat from one side to the other in sequence.

5. The temperature control and compensation device for the PCR instrument according to any one of claims 2-4, characterized in that, The heating power of the heating element gradually increases from the inside to the outside.

6. The temperature control and compensation device for the PCR instrument according to claim 1, characterized in that, The first column has multiple components, and adjacent first columns are connected by a connecting part, the top of which is lower than the top of the first column.

7. The temperature control and compensation device for the PCR instrument according to claim 1, characterized in that, The temperature control module has a first mounting slot at its bottom, which is used to place the first temperature sensor.

8. The temperature control and compensation device for the PCR instrument according to claim 1, characterized in that, It also includes a second temperature sensor, which is located on top of the heat cover.

9. A PCR instrument, characterized in that, Includes the temperature control compensation device of the PCR instrument according to any one of claims 1-8.

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