A biological agent, and a method of preparing and using the same

By using a compound microbial strain of Pediococcus cellarus, Candida tropicalis, and Bacillus amyloliquefaciens for anaerobic fermentation, the problem of conversion of chrysanthemum waste with high moisture content was solved, the yield of crude protein and flavonoids was increased, and the nutritional value of chrysanthemum waste was enhanced.

CN115747118BActive Publication Date: 2026-05-29ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
Filing Date
2022-12-08
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

How to improve the crude protein and flavonoid yield of chrysanthemum waste, especially for chrysanthemum waste with high moisture content, which is difficult to effectively convert with existing fermentation technology.

Method used

Anaerobic fermentation was carried out using a compound microbial strain of Pediococcus cellarus, Candida tropicalis, and Bacillus amyloliquefaciens. The ratio of chrysanthemum waste to carbon source was 7-9:1-3, the inoculum amount was 6-10%, the fermentation time was 6-10 days, and the fermentation was carried out at room temperature.

Benefits of technology

It significantly increased the crude protein and flavonoid content of chrysanthemum waste, provided abundant free amino acids, and improved fermentation efficiency and product quality.

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Abstract

The application belongs to the field of biology and discloses a kind of biological agent, and the substrate formed by chrysanthemum waste and carbon source is anaerobically fermented by using compound bacteria;The compound bacteria are Pediococcus acetii, Candida tropicalis and Bacillus amyloliquefaciens.The biological agent has higher fermentation efficiency on chrysanthemum waste with high moisture content, and can improve the yield of crude protein and flavonoids;Meanwhile, the preparation method and application of the agent are also provided.
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