A base editing tool

By combining the SunTag and PE2 systems, the connection between the GCN4 peptide and Cas9 nickase and reverse transcriptase was optimized, expanding the editing window and improving editing efficiency. This addresses the shortcomings of existing base editing systems in terms of editing efficiency and accuracy, achieving more efficient gene editing results.

CN115772523BActive Publication Date: 2026-07-03ZHUHAI SHU TONG MEDICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHUHAI SHU TONG MEDICAL TECH CO LTD
Filing Date
2021-08-10
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing base editing systems have shortcomings in terms of editing efficiency and accuracy. In particular, different PE systems have inconsistent editing efficiency for the same site, which affects the accuracy of gene editing.

Method used

By combining the SunTag system with the PE2 system to form the SunTag-PE2 system, the editing window is expanded and editing efficiency is improved by optimizing the connection between the GCN4 peptide and the Cas9 nickase and reverse transcriptase.

Benefits of technology

It significantly improves the accuracy and precision of base editing, enhances the selectivity of gene editing, and provides more options for gene editing.

✦ Generated by Eureka AI based on patent content.

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Abstract

This invention relates to a base editing tool. To improve the efficiency and accuracy of target base editing in the existing PE2 system, this invention provides a novel base editing tool comprising the SunTag system and the PE2 system. This invention, for the first time, utilizes the results of the SunTag and PE systems, employing the SunTag system and a more efficient version of PE2 to construct the SunTag-PE2 system. After optimization, the tool is compared with the original PE2 system on HEK293T cells. The proposed base editing tool further improves the accuracy and precision of the PE2 system, providing more options for gene editing. This technology will promote the rapid development of life science research, agricultural production, and biomedicine in my country and worldwide, and has significant application value.
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